Drs

Summary

Gene Symbol: Drs
Description: Drosomycin
Alias: BcDNA:LP03851, CG10810, Crp, DIM 19, DIM 21, DRO, DROM, DROS, DRS, Dmel\CG10810, Drm, Drom, Dros, Droso, IM21, dmy1, drm, drom, dros, drs, drsm, CG10810 gene product from transcript CG10810-RA, CG10810-PA, Cysteine-rich-protein, Drosomycin-B, Drs-PA, cysteine-rich peptide, drosomcycin, drosomyci, drosomycin, drosomycin precursor, drosomycine, drosomyocin, drosomysin, drosophila immune induced molecule 19, immune induced molecule 21
Species: fruit fly

Top Publications

  1. ncbi The dorsoventral regulatory gene cassette spätzle/Toll/cactus controls the potent antifungal response in Drosophila adults
    B Lemaitre
    Institut de Biologie Moléculaire at Cellulaire, UPR 9022 du Centre National de la Recherche Scientifique, Strasbourg, France
    Cell 86:973-83. 1996
  2. ncbi Two proteases defining a melanization cascade in the immune system of Drosophila
    Huaping Tang
    Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, Connecticut 06520, USA
    J Biol Chem 281:28097-104. 2006
  3. pmc The immune response attenuates growth and nutrient storage in Drosophila by reducing insulin signaling
    Justin R DiAngelo
    The Institute for Diabetes, Obesity and Metabolism, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
    Proc Natl Acad Sci U S A 106:20853-8. 2009
  4. ncbi Binding of the Drosophila cytokine Spätzle to Toll is direct and establishes signaling
    Alexander N R Weber
    Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Cambridge CB2 1GA, United Kingdom
    Nat Immunol 4:794-800. 2003
  5. ncbi Expression and evolution of the Drosophila attacin/diptericin gene family
    M Hedengren
    Umea Centre for Molecular Pathogenesis, Umea University, Umea, S 901 87, Sweden
    Biochem Biophys Res Commun 279:574-81. 2000
  6. pmc The phytopathogenic bacteria Erwinia carotovora infects Drosophila and activates an immune response
    A Basset
    Centre de Genetique Moleculaire, Centre National de la Recherche Scientifique, F 91198 Gif sur Yvette Cedex, France
    Proc Natl Acad Sci U S A 97:3376-81. 2000
  7. ncbi dUbc9 negatively regulates the Toll-NF-kappa B pathways in larval hematopoiesis and drosomycin activation in Drosophila
    Hsiling Chiu
    Department of Functional Genomics, Novartis Institutes for BioMedical Research, 100 Technology Square Bldg 601 Rm 6404, Cambridge, MA 02139, USA
    Dev Biol 288:60-72. 2005
  8. pmc Discerning the complexity of community interactions using a Drosophila model of polymicrobial infections
    Christopher D Sibley
    Department of Microbiology and Infectious Diseases, University of Calgary, Calgary, Alberta, Canada
    PLoS Pathog 4:e1000184. 2008
  9. ncbi Interactions between the cellular and humoral immune responses in Drosophila
    M Elrod-Erickson
    Whitehead Institute, Cambridge, MA 02142, USA
    Curr Biol 10:781-4. 2000
  10. pmc The Drosophila caspase Dredd is required to resist gram-negative bacterial infection
    F Leulier
    Centre de Genetique Moleculaire, Centre National de la Recherche Scientifique, France
    EMBO Rep 1:353-8. 2000

Research Grants

  1. The Role of Peroxiredoxins in Immunity and Aging
    SVETLANA RADYUK; Fiscal Year: 2006
  2. Using Drosphila Macrophages to Study Innate Immunity
    David Schneider; Fiscal Year: 2007
  3. REGULATION OF IMMUNE RESPONSE AND CELL GROWTH
    DEBORAH KIMBRELL; Fiscal Year: 2001

Patents

  1. CHIMERIC GENE CODING FOR DROSOMICINE, VECTOR CONTAINING IT AND PRODUCTION OF TRANSGENIC PLANTS RESISTANT TO DISEASES

Scientific Experts

Detail Information

Publications142 found, 100 shown here

  1. ncbi The dorsoventral regulatory gene cassette spätzle/Toll/cactus controls the potent antifungal response in Drosophila adults
    B Lemaitre
    Institut de Biologie Moléculaire at Cellulaire, UPR 9022 du Centre National de la Recherche Scientifique, Strasbourg, France
    Cell 86:973-83. 1996
    ..for dorsal) and the extracellular Toll ligand, spätzle, control expression of the antifungal peptide gene drosomycin in adults...
  2. ncbi Two proteases defining a melanization cascade in the immune system of Drosophila
    Huaping Tang
    Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, Connecticut 06520, USA
    J Biol Chem 281:28097-104. 2006
    ..We have also shown that the melanization reaction activated by MP1 and MP2 plays an important role in augmenting the effectiveness of other immune reactions, thereby promoting resistance of Drosophila to microbial infection...
  3. pmc The immune response attenuates growth and nutrient storage in Drosophila by reducing insulin signaling
    Justin R DiAngelo
    The Institute for Diabetes, Obesity and Metabolism, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
    Proc Natl Acad Sci U S A 106:20853-8. 2009
    ..These data suggest that communication between these two regulatory systems evolved as a means to divert energy in times of need from organismal growth to the acute requirement of combating infection...
  4. ncbi Binding of the Drosophila cytokine Spätzle to Toll is direct and establishes signaling
    Alexander N R Weber
    Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Cambridge CB2 1GA, United Kingdom
    Nat Immunol 4:794-800. 2003
    ..These results show that, in contrast to the human Toll-like receptors, Drosophila Toll requires only an endogenous protein ligand for activation and signaling...
  5. ncbi Expression and evolution of the Drosophila attacin/diptericin gene family
    M Hedengren
    Umea Centre for Molecular Pathogenesis, Umea University, Umea, S 901 87, Sweden
    Biochem Biophys Res Commun 279:574-81. 2000
    ..This induction is reduced in imd mutants, and unexpectedly also in Tl(-) mutants. The 18w mutation particularly affects the induction of AttC, which may be a useful marker for 18w signaling...
  6. pmc The phytopathogenic bacteria Erwinia carotovora infects Drosophila and activates an immune response
    A Basset
    Centre de Genetique Moleculaire, Centre National de la Recherche Scientifique, F 91198 Gif sur Yvette Cedex, France
    Proc Natl Acad Sci U S A 97:3376-81. 2000
    ....
  7. ncbi dUbc9 negatively regulates the Toll-NF-kappa B pathways in larval hematopoiesis and drosomycin activation in Drosophila
    Hsiling Chiu
    Department of Functional Genomics, Novartis Institutes for BioMedical Research, 100 Technology Square Bldg 601 Rm 6404, Cambridge, MA 02139, USA
    Dev Biol 288:60-72. 2005
    ..In the larval fat body, dUbc9 negatively regulates the expression of the antifungal peptide gene drosomycin, which is constitutively expressed in dUbc9 mutants in the absence of immune challenge...
  8. pmc Discerning the complexity of community interactions using a Drosophila model of polymicrobial infections
    Christopher D Sibley
    Department of Microbiology and Infectious Diseases, University of Calgary, Calgary, Alberta, Canada
    PLoS Pathog 4:e1000184. 2008
    ..aeruginosa...
  9. ncbi Interactions between the cellular and humoral immune responses in Drosophila
    M Elrod-Erickson
    Whitehead Institute, Cambridge, MA 02142, USA
    Curr Biol 10:781-4. 2000
    ....
  10. pmc The Drosophila caspase Dredd is required to resist gram-negative bacterial infection
    F Leulier
    Centre de Genetique Moleculaire, Centre National de la Recherche Scientifique, France
    EMBO Rep 1:353-8. 2000
    ....
  11. ncbi Upregulation of genes belonging to the drosomycin family in diapausing adults of Drosophila triauraria
    S Daibo
    Graduate School of Environmental Earth Science, Hokkaido University, Sapporo, Hokkaido 060 0810, Japan
    Gene 278:177-84. 2001
    ..hybridization have similarity to genes encoding antifungal peptides of Drosophila melanogaster, members of the drosomycin family (drosomycin, CG10812, CG10813, CG10815 and CG11520)...
  12. pmc Nitric oxide contributes to induction of innate immune responses to gram-negative bacteria in Drosophila
    Edan Foley
    Department of Biochemistry and Biophysics, University of California San Francisco, San Francisco, California 94143, USA
    Genes Dev 17:115-25. 2003
    ..We propose that NO mediates an early step of the signal transduction pathway, inducing the innate immune response upon natural infection with gram-negative bacteria...
  13. pmc Uptake of the necrotic serpin in Drosophila melanogaster via the lipophorin receptor-1
    Sandra Fausia Soukup
    Functional Genomics Unit, CIC bioGUNE, Derio, Spain
    PLoS Genet 5:e1000532. 2009
    ..The scavenging of serpin/proteinase complexes may be a critical step in the regulation of proteolytic cascades...
  14. ncbi Activation of Drosophila Toll during fungal infection by a blood serine protease
    Petros Ligoxygakis
    Institut de Biologie Moleculaire et Cellulaire, UPR 9022 du CNRS, 15 rue R Descartes, F67084 Strasbourg Cedex, France
    Science 297:114-6. 2002
    ..We show that ethylmethane sulfonate-induced mutations in the persephone gene, which encodes a previously unknown serine protease, block induction of the Toll pathway by fungi and resistance to this type of infection...
  15. ncbi Immunity regulatory DNAs share common organizational features in Drosophila
    Kate Senger
    Department of Molecular and Cellular Biology, Division of Genetics and Development, University of California, Berkeley, Berkeley, CA 94720, USA
    Mol Cell 13:19-32. 2004
    ..Aspects of this "regulatory code" are essential for the immune response. These results suggest that immunity regulatory DNAs contain constrained organizational features, which may be a general property of eukaryotic enhancers...
  16. pmc Circadian regulation in the ability of Drosophila to combat pathogenic infections
    Jung Eun Lee
    Graduate Program in Biochemistry, Center for Advanced Biotechnology and Medicine, Rutgers University, 679 Hoes Lane, Piscataway, New Jersey 08854, USA
    Curr Biol 18:195-9. 2008
    ..Our findings suggest that medical intervention strategies incorporating chronobiological considerations could enhance the innate immune response, boosting the efficacy of combating pathogenic infections...
  17. ncbi Signaling role of hemocytes in Drosophila JAK/STAT-dependent response to septic injury
    Herve Agaisse
    Department of Genetics, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA
    Dev Cell 5:441-50. 2003
    ....
  18. pmc Prophenoloxidase activation is not required for survival to microbial infections in Drosophila
    Vincent Leclerc
    UPR9022 du CNRS, IBMC, 15 rue Descartes, 67084 Strasbourg, France
    EMBO Rep 7:231-5. 2006
    ..This raises the question with regard to the precise function of phenoloxidase activation in defence, if any...
  19. pmc Drosophila melanogaster mounts a unique immune response to the Rhabdovirus sigma virus
    C W Tsai
    Department of Entomology, The Ohio State University OARDC, Wooster, Ohio 44691, USA
    Appl Environ Microbiol 74:3251-6. 2008
    ..SIGMAV infection did not induce PGRP-SA and the AMP genes Drosomycin-B, Metchnikowin, and Defensin that are upregulated in DCV and/or DXV infections...
  20. pmc Peptidoglycan recognition protein-SD provides versatility of receptor formation in Drosophila immunity
    Lihui Wang
    Genetics Unit, Department of Biochemistry, South Parks Road, University of Oxford, Oxford OX1 3QU, United Kingdom
    Proc Natl Acad Sci U S A 105:11881-6. 2008
    ..These results indicate that the interplay between the binding affinities of different PGRPs provides sufficient flexibility for the recognition of the highly diverse Gram-positive PG...
  21. ncbi Insect immunity. Septic injury of Drosophila induces the synthesis of a potent antifungal peptide with sequence homology to plant antifungal peptides
    P Fehlbaum
    Institut de Biologie Moleculaire et Cellulaire, UPR 9022 Centre National de la Recherche Scientifique Réponse Immunitaire et Développement chez les Insectes, Strasbourg, France
    J Biol Chem 269:33159-63. 1994
    ..This novel inducible peptide, which we propose to name drosomycin, shows a significant homology with a family of 5-kDa cysteine-rich plant antifungal peptides recently isolated ..
  22. pmc Constitutive expression of a single antimicrobial peptide can restore wild-type resistance to infection in immunodeficient Drosophila mutants
    Phoebe Tzou
    Centre de Genetique Moleculaire, Centre National de la Recherche Scientifique, 91198 Gif sur Yvette, France
    Proc Natl Acad Sci U S A 99:2152-7. 2002
    ....
  23. pmc Critical evaluation of the role of the Toll-like receptor 18-Wheeler in the host defense of Drosophila
    Petros Ligoxygakis
    Institut de Biologie Moléculaire and Cellulaire, Strasbourg Cedex, France
    EMBO Rep 3:666-73. 2002
    ..18-Wheeler does not qualify as a pattern recognition receptor of Gram-negative bacteria...
  24. ncbi Splice-activated UAS hairpin vector gives complete RNAi knockout of single or double target transcripts in Drosophila melanogaster
    Jean Marc Reichhart
    UPR 9022 C N R S, Institut de Biologie Moleculaire et Cellulaire, Strasbourg, France
    Genesis 34:160-4. 2002
  25. pmc Activation of the innate immunity in Drosophila by endogenous chromosomal DNA that escaped apoptotic degradation
    Naomi Mukae
    Department of Genetics, Osaka University Medical School, Osaka 565 0871, Japan
    Genes Dev 16:2662-71. 2002
    ..These results indicated that CAD and DNase II work independently to degrade chromosomal DNA during apoptosis, and if the DNA is left undigested, it can activate the innate immunity in Drosophila...
  26. pmc The evolution of antifungal peptides in Drosophila
    Francis M Jiggins
    Institute of Evolutionary Biology, School of Biological Sciences, University of Edinburgh, Ashworth Lab, King s Buildings, West Mains Road, Edinburgh EH9 3JT, Scotland
    Genetics 171:1847-59. 2005
    ..the selection pressures acting on Drosophila antifungal peptides (drosomycins) from both the divergence of drosomycin genes within and between five species of Drosophila and polymorphism data from Drosophila simulans and D...
  27. ncbi Drosophila immunity: a large-scale in vivo RNAi screen identifies five serine proteases required for Toll activation
    Zakaria Kambris
    Centre de Genetique Moleculaire, CNRS, Gif sur Yvette, France
    Curr Biol 16:808-13. 2006
    ..These results demonstrate the existence of a common cascade of SPs upstream of Spz, integrating signals sent by various secreted recognition molecules via more specialized SPs...
  28. pmc Functional analysis of Toll-related genes in Drosophila
    Yoshimasa Yagi
    Division of Biological Science, Graduate School of Science, Nagoya University, Furo cho, Chikusa ku, Nagoya 464 8602, Japan
    Dev Growth Differ 52:771-83. 2010
    ..Overall our results suggest that some of the Toll-related proteins, such as 18W, Toll-7 and Toll-8, may have redundant functions in regulating developmental processes...
  29. pmc Lack of an antibacterial response defect in Drosophila Toll-9 mutant
    Karine Narbonne-Reveau
    Institut de Biologie du Developpement de Marseille Luminy, CNRS UMR 6216 Aix Marseille II University, Campus de Luminy, Marseille, France
    PLoS ONE 6:e17470. 2011
    ..Our results suggest that Toll-9 is neither required to maintain a basal anti-microbial response nor to mount an efficient immune response to bacterial infection...
  30. pmc Analysis of the Drosophila host defense in domino mutant larvae, which are devoid of hemocytes
    A Braun
    Unité Propre de Recherche 9022, Réponse Immunitaire et Développement chez les Insectes, Institut de Biologie Moléculaire et Cellulaire 15, rue René Descartes, F 67084 Strasbourg CEDEX, France
    Proc Natl Acad Sci U S A 95:14337-42. 1998
    ..By working in synergy, they provide Drosophila a highly effective defense against injury and/or infection...
  31. pmc A mosaic analysis in Drosophila fat body cells of the control of antimicrobial peptide genes by the Rel proteins Dorsal and DIF
    P Manfruelli
    Institut de Biologie Moleculaire et Cellulaire, UPR 9022 du Centre National de la Recherche Scientifique, 15, rue René Descartes, F 67084 Strasbourg CEDEX, France
    EMBO J 18:3380-91. 1999
    Expression of the gene encoding the antifungal peptide Drosomycin in Drosophila adults is controlled by the Toll signaling pathway...
  32. ncbi Constitutive activation of toll-mediated antifungal defense in serpin-deficient Drosophila
    E A Levashina
    UPR 9022 CNRS, Institut de Biologie Moleculaire et Cellulaire, 15 rue Rene Descartes, Strasbourg 67084, France
    Science 285:1917-9. 1999
    ..blood serine protease inhibitor, Spn43Ac, was shown to lead to constitutive expression of the antifungal peptide drosomycin, and this effect was mediated by the spaetzle and Toll gene products...
  33. ncbi Relish, a central factor in the control of humoral but not cellular immunity in Drosophila
    M Hedengren
    Umeå Center for Molecular Pathogenesis, Umea University, Sweden
    Mol Cell 4:827-37. 1999
    ..Our results illustrate the importance of the humoral response in Drosophila immunity and demonstrate that Relish plays a key role in this response...
  34. ncbi Gram-negative bacteria-binding protein, a pattern recognition receptor for lipopolysaccharide and beta-1,3-glucan that mediates the signaling for the induction of innate immune genes in Drosophila melanogaster cells
    Y S Kim
    Laboratory of Immunology, BK21 Center for Medical Science and Medical Research Center, Yonsei University College of Medicine, 134 Shinchon dong, CPO Box 8044, Seoul, South Korea
    J Biol Chem 275:32721-7. 2000
    ....
  35. ncbi Role of Drosophila IKK gamma in a toll-independent antibacterial immune response
    S Rutschmann
    Institut de Biologie Moleculaire et Cellulaire, UPR 9022 du CNRS, 15, rue R Descartes, F67084 Strasbourg, France
    Nat Immunol 1:342-7. 2000
    ..Thus, in contrast to the vertebrate inflammatory response, IKK gamma is required for the activation of only one immune signaling pathway in Drosophila...
  36. ncbi Drosophila immune deficiency (IMD) is a death domain protein that activates antibacterial defense and can promote apoptosis
    P Georgel
    Institut de Biologie Moleculaire et Cellulaire, UPR 9022 du CNRS, Strasbourg, France
    Dev Cell 1:503-14. 2001
    ..We also show that imd is involved in the apoptotic response to UV irradiation. These data raise the possibility that antibacterial response and apoptosis share common control elements in Drosophila...
  37. ncbi Drosophila MyD88 is required for the response to fungal and Gram-positive bacterial infections
    Servane Tauszig-Delamasure
    UPR9022 du Centre National de la Recherche Scientifique, Institut de Biologie Moleculaire et Cellulaire, 15, rue René Descartes, 67000 Strasbourg, France
    Nat Immunol 3:91-7. 2002
    ..Overexpression of DmMyD88 was sufficient to induce expression of the antifungal peptide Drosomycin, and induction of Drosomycin was markedly reduced in DmMyD88-mutant flies...
  38. ncbi The Drosophila immune response against Gram-negative bacteria is mediated by a peptidoglycan recognition protein
    Marie Gottar
    UPR 9022 du Centre National de la Recherche Scientifique, Institut de Biologie Moleculaire et Cellulaire, 15 rue Rene Descartes, F67084 Strasbourg, Cedex, France
    Nature 416:640-4. 2002
    ..The data on PGRP-SA with respect to the response to Gram-positive infections, together with the present report, indicate that the PGRP family has a principal role in sensing microbial infections in Drosophila...
  39. pmc Directed expression of the HIV-1 accessory protein Vpu in Drosophila fat-body cells inhibits Toll-dependent immune responses
    François Leulier
    Centre de Génétique Moléculaire Centre National de la Recherche Scientifique, Batiment 26, Avenue de la Terrasse, 91198 Gif sur Yvette, France
    EMBO Rep 4:976-81. 2003
    ....
  40. pmc Silencing of Toll pathway components by direct injection of double-stranded RNA into Drosophila adult flies
    Akira Goto
    UPR 9022 du Centre National de la Recherche Scientifique, Institut de Biologie Moleculaire et Cellulaire, 15 rue Rene Descartes, F 67084 Strasbourg CEDEX, France
    Nucleic Acids Res 31:6619-23. 2003
    ....
  41. ncbi The immune response of Drosophila
    Jules A Hoffmann
    Institut de Biologie Moleculaire et Cellulaire du CNRS, 67084 Strasbourg Cedex, France
    Nature 426:33-8. 2003
    ..Recent progress in research on Drosophila immune defence provides evidence for similarities and differences between Drosophila immune responses and mammalian innate immunity...
  42. ncbi A serpin regulates dorsal-ventral axis formation in the Drosophila embryo
    Petros Ligoxygakis
    Genetics Unit, Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK
    Curr Biol 13:2097-102. 2003
    ..Since this serpin has been recently shown to restrain an immune reaction in the blood of Drosophila, it demonstrates that proteolysis can be regulated by the same serpin in different biological contexts...
  43. pmc The Toll pathway is important for an antiviral response in Drosophila
    Robert A Zambon
    Center for Biosystems Research, University of Maryland Biotechnology Institute, College Park, MD 20742, USA
    Proc Natl Acad Sci U S A 102:7257-62. 2005
    ..Additionally, our results demonstrate the validity of using a genetic approach to identify genes and pathways used in viral innate immune responses in Drosophila...
  44. ncbi PGRP-LC and PGRP-LE have essential yet distinct functions in the drosophila immune response to monomeric DAP-type peptidoglycan
    Takashi Kaneko
    Division of Infectious Disease, Department of Medicine, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
    Nat Immunol 7:715-23. 2006
    ..These data demonstrate that like mammals, drosophila use both extracellular and intracellular receptors, which have conserved signaling mechanisms, for innate immune recognition...
  45. doi Drosophila Serpin-28D regulates hemolymph phenoloxidase activity and adult pigmentation
    Christoph Scherfer
    Centre de Genetique Moleculaire, CNRS, 91198 Gif sur Yvette, France
    Dev Biol 323:189-96. 2008
    ..This study further highlights the complexity of the proPO cascade that can be differentially regulated in different tissues during development...
  46. doi The DExD/H-box helicase Dicer-2 mediates the induction of antiviral activity in drosophila
    Safia Deddouche
    Unité Propre de Recherche 9022, Centre National de la Recherche Scientifique, Institut de Biologie Moleculaire et Cellulaire, 67084 Strasbourg, France
    Nat Immunol 9:1425-32. 2008
    ..We propose that this family represents an evolutionary conserved set of sensors that detect viral nucleic acids and direct antiviral responses...
  47. pmc Solution structure of drosomycin, the first inducible antifungal protein from insects
    C Landon
    Centre de Biophysique Moléculaire UPR 4301 CNRS, Orleans, France
    Protein Sci 6:1878-84. 1997
    b>Drosomycin is the first antifungal protein characterized recently among the broad family of inducible peptides and proteins produced by insects to respond to bacterial or septic injuries...
  48. ncbi Two distinct pathways can control expression of the gene encoding the Drosophila antimicrobial peptide metchnikowin
    E A Levashina
    Réponse Immunitaire et Développement chez les Insectes, UPR 9022 du CNRS, Institut de Biologie Moleculaire et Cellulaire, 15 rue Rene Descartes, Strasbourg, 67000, France
    J Mol Biol 278:515-27. 1998
    ..5 kb of metchnikowin gene upstream sequences indicates that this fragment is able to confer full immune inducibility and tissue specificity of expression on the transgene...
  49. pmc The antibacterial arm of the drosophila innate immune response requires an IkappaB kinase
    Y Lu
    Molecular Biology Program, Sloan Kettering Institute, New York, New York 10021, USA
    Genes Dev 15:104-10. 2001
    ..The ird5 gene encodes a Drosophila homolog of mammalian IkappaB kinases (IKKs). The ird5 phenotype and sequence suggest that the gene is specifically required for the activation of Relish, a Drosophila NF-kappaB family member...
  50. pmc Drosophila MyD88 is an adapter in the Toll signaling pathway
    T Horng
    Howard Hughes Medical Institute and Section of Immunobiology, Yale University School of Medicine, New Haven, CT 06520, USA
    Proc Natl Acad Sci U S A 98:12654-8. 2001
    ..Expression of dMyD88 in S2 cells strongly induced activity of a Drosomycin reporter gene, whereas a dominant-negative version of dMyD88 potently inhibited Toll-mediated signaling...
  51. ncbi The Drosophila immune defense against gram-negative infection requires the death protein dFADD
    Silvia Naitza
    Institut de Biologie Moleculaire et Cellulaire, UPR 9022 du CNRS, 15 rue Rene Descartes, 67084 Cedex, Strasbourg, France
    Immunity 17:575-81. 2002
    ..By genetic analysis we show that dFADD acts downstream of IMD in the pathway that controls inducibility of the antibacterial peptide genes...
  52. pmc DmMyD88 controls dorsoventral patterning of the Drosophila embryo
    Zakaria Kambris
    Institut de Biologie Moleculaire et Cellulaire, UPR 9022 du CNRS, 15, rue René Descartes, 67084 Strasbourg Cedex, France
    EMBO Rep 4:64-9. 2003
    ..The DmMyD88 mutation blocks the ventralizing activity of a gain-of-function Toll mutation. These results show that DmMyD88 encodes an essential component of the Toll pathway in dorsoventral pattern formation...
  53. ncbi The Drosophila immune system detects bacteria through specific peptidoglycan recognition
    François Leulier
    Centre de Génétique Moléculaire du CNRS, F 91198 Gif sur Yvette, France
    Nat Immunol 4:478-84. 2003
    ..Thus, the ability of Drosophila to discriminate between Gram-positive and Gram-negative bacteria relies on the recognition of specific forms of peptidoglycan...
  54. ncbi In vivo RNA interference analysis reveals an unexpected role for GNBP1 in the defense against Gram-positive bacterial infection in Drosophila adults
    Sebastien Pili-Floury
    Centre de Genetique Moleculaire, CNRS, F 91198 Gif sur Yvette Cedex, France
    J Biol Chem 279:12848-53. 2004
    ..to Gram-positive bacterial infection and reduces the induction of the antifungal peptide encoding gene Drosomycin after infection by Gram-positive bacteria but not after fungal infection...
  55. pmc A Drosophila pattern recognition receptor contains a peptidoglycan docking groove and unusual L,D-carboxypeptidase activity
    Chung I Chang
    Howard Hughes Medical Institute and Department of Biochemistry, University of Texas Southwestern Medical Center, Dallas, Texas, USA
    PLoS Biol 2:E277. 2004
    ....
  56. ncbi Function of the drosophila pattern-recognition receptor PGRP-SD in the detection of Gram-positive bacteria
    Vincent Bischoff
    Institut de Biologie Moleculaire et Cellulaire, CNRS UPR 9022, 15 rue Rene Descartes, Universite Louis Pasteur, 67084 Strasbourg Cedex, France
    Nat Immunol 5:1175-80. 2004
    ..These data indicate that PGRP-SD can function as a receptor for Gram-positive bacteria and shows partial redundancy with the PGRP-SA-GNBP1 complex...
  57. ncbi Eater, a transmembrane protein mediating phagocytosis of bacterial pathogens in Drosophila
    Christine Kocks
    Laboratory of Developmental Immunology, Department of Pediatrics, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts 02114, USA
    Cell 123:335-46. 2005
    ..Our results suggest that Eater is a major phagocytic receptor for a broad range of bacterial pathogens in Drosophila and provide a powerful model to address the role of phagocytosis in vivo...
  58. ncbi Functional divergence of six isoforms of antifungal peptide Drosomycin in Drosophila melanogaster
    Wan Ying Yang
    Department of Sericulture Science, College of Animal Science, South China Agricultural University, 510642, Guangzhou, PR China
    Gene 379:26-32. 2006
    b>Drosomycin (Drs) gene encodes a 44-residue inducible antifungal peptide, Drosomycin, in Drosophila melanogaster...
  59. ncbi Expression and regulation of Spätzle-processing enzyme in Drosophila
    Shai Mulinari
    Department of Experimental Medical Science and Lund Strategic Research Center for Stem Cell Biology and Cell Therapy, Lund University, BMC B13, Klinikgatan 26, 22184 Lund, Sweden
    FEBS Lett 580:5406-10. 2006
    ....
  60. pmc Sensing of Gram-positive bacteria in Drosophila: GNBP1 is needed to process and present peptidoglycan to PGRP-SA
    Lihui Wang
    Genetics Unit, Department of Biochemistry, University of Oxford, Oxford, UK
    EMBO J 25:5005-14. 2006
    ..We propose a model whereby GNBP1 presents a processed form of PG for sensing by PGRP-SA and that a tripartite interaction between these proteins and PG is essential for downstream signaling...
  61. ncbi Realized immune response is enhanced in long-lived puc and chico mutants but is unaffected by dietary restriction
    Sergiy Libert
    Program in Cell and Molecular Biology, Baylor College of Medicine, One Baylor Plaza, N820, Houston TX, 77030, United States
    Mol Immunol 45:810-7. 2008
    ..Such upregulation is not observed during DR, suggesting the presence of a mechanism that suppresses immune activity in diet-restricted animals...
  62. doi Innate immune homeostasis by the homeobox gene caudal and commensal-gut mutualism in Drosophila
    Ji Hwan Ryu
    Division of Molecular Life Science, Ewha Woman s University and National Creative Research Initiative Center for Symbiosystem, Seoul 120 750, South Korea
    Science 319:777-82. 2008
    ..These results reveal that a specific genetic deficiency within a host can profoundly influence the gut commensal microbial community and host physiology...
  63. pmc Drosophila melanogaster as a model host to dissect the immunopathogenesis of zygomycosis
    Georgios Chamilos
    Department of Infectious Diseases, University of Texas M D Anderson Cancer Center, Houston, TX 77030, USA
    Proc Natl Acad Sci U S A 105:9367-72. 2008
    ..exhibited increased susceptibility to Zygomycetes, whereas constitutive overexpression of the antifungal peptide Drosomycin in transgenic flies partially restored resistance to zygomycosis. D...
  64. doi Identification of a cis-regulatory element required for 20-hydroxyecdysone enhancement of antimicrobial peptide gene expression in Drosophila melanogaster
    Z Zhang
    Department of Entomology, College of Agriculture, University of Kentucky, Lexington, KY, USA
    Insect Mol Biol 18:595-605. 2009
    ..also enhanced peptidoglycan-induced expression of four other antimicrobial peptide (AMP) genes--drosomycin, attacin-A, metchnikowin and cecropin A1...
  65. pmc Long-range activation of systemic immunity through peptidoglycan diffusion in Drosophila
    Mathilde Gendrin
    Global Health Institute, EPFL, Lausanne, Switzerland
    PLoS Pathog 5:e1000694. 2009
    ..Genital infection is thus an intriguing new model for studying the systemic immune response to local epithelial infections and a potential route of entry for naturally occurring pathogens of Drosophila...
  66. doi Pellino enhances innate immunity in Drosophila
    Amirhossein Haghayeghi
    Department of Biology, McGill University, 1205 Dr Penfield Avenue, Montreal, Que, Canada H3A 1B1
    Mech Dev 127:301-7. 2010
    ..Here we show that Pellino, a highly conserved protein interacting with activated Pelle/IRAK, acts as a positive regulator of innate immunity in Drosophila...
  67. pmc Chronic activation of the epithelial immune system of the fruit fly's salivary glands has a negative effect on organismal growth and induces a peculiar set of target genes
    Ahmed Abdelsadik
    Christian Albrechts University of Kiel, Zoophysiology, Kiel, Germany
    BMC Genomics 11:265. 2010
    ..Our major goal was to elucidate if the fly's salivary glands are able to mount an immune response and what the major characteristics of this immune response are...
  68. pmc Heterodimers of NF-kappaB transcription factors DIF and Relish regulate antimicrobial peptide genes in Drosophila
    Takahiro Tanji
    Department of Cell Biology, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Proc Natl Acad Sci U S A 107:14715-20. 2010
    ..The DIF and Relish complex is detectable in whole animal extracts, suggesting that this heterodimer may function in vivo to increase the spectrum and level of antimicrobial peptide production in response to different infections...
  69. pmc Comparative pathology of bacteria in the genus Providencia to a natural host, Drosophila melanogaster
    Madeline R Galac
    Field of Genetics and Development, 3125 Comstock Hall, Cornell University, Ithaca, NY 14850, USA
    Microbes Infect 13:673-83. 2011
    ..D. melanogaster and Providencia provide a powerful system for studying general host-pathogen interactions, and for understanding how the well-studied immune model host D. melanogaster interacts with its natural bacterial pathogens...
  70. pmc Drosophila immunity: analysis of PGRP-SB1 expression, enzymatic activity and function
    Anna Zaidman-Rémy
    Centre de Génétique Moléculaire du CNRS, Gif sur Yvette, France
    PLoS ONE 6:e17231. 2011
    ..Possible immune functions of PGRP-SB1 are discussed...
  71. pmc A recessive mutation, immune deficiency (imd), defines two distinct control pathways in the Drosophila host defense
    B Lemaitre
    Institut de Biologie Moleculaire et Cellulaire, Unité Propre de Recherche 9022 du Centre National de la Recherche Scientifique, Strasbourg, France
    Proc Natl Acad Sci U S A 92:9465-9. 1995
    ..We also report that, in contrast to the antibacterial peptides, the antifungal peptide drosomycin remains inducible in a homozygous imd mutant background...
  72. pmc Drosophila host defense: differential induction of antimicrobial peptide genes after infection by various classes of microorganisms
    B Lemaitre
    Institut de Biologie Moleculaire et Cellulaire, Unité Propre de Recherche 9022 du Centre National de la Recherche Scientifique, 15 rue Rene Descartes, 67084 Strasbourg Cedex, France
    Proc Natl Acad Sci U S A 94:14614-9. 1997
    ..This response is mediated through the selective activation of the Toll pathway...
  73. pmc A drosomycin-GFP reporter transgene reveals a local immune response in Drosophila that is not dependent on the Toll pathway
    D Ferrandon
    Institut de Biologie Moleculaire et Cellulaire, UPR 9022 du Centre National de la Recherche Scientifique, 15, rue René Descartes, F67084 Strasbourg, France
    EMBO J 17:1217-27. 1998
    ..One of these peptides, drosomycin, is active primarily against fungi...
  74. pmc Differential display of peptides induced during the immune response of Drosophila: a matrix-assisted laser desorption ionization time-of-flight mass spectrometry study
    S Uttenweiler-Joseph
    Institut de Biologie Moleculaire et Cellulaire, Unité Propre de Recherche 9022 du Centre National de la Recherche Scientifique, 15, rue René Descartes, 67084 Strasbourg Cedex, France
    Proc Natl Acad Sci U S A 95:11342-7. 1998
    ..Finally, molecular cloning and Northern blot analyses revealed that one of the DIMs is produced as a prepropeptide and is inducible on a bacterial challenge...
  75. pmc Toll receptor-mediated Drosophila immune response requires Dif, an NF-kappaB factor
    X Meng
    Program in Molecular Medicine, Department of Cell Biology, and Department of Biochemistry and Molecular Biology, University of Massachusetts Medical School, Worcester, Massachusetts 01605 USA
    Genes Dev 13:792-7. 1999
    ..The results show that the presence of Dif without Dorsal is sufficient to mediate the induction of drosomycin and defensin...
  76. ncbi Phylogenetic perspectives in innate immunity
    J A Hoffmann
    Institute of Molecular and Cellular Biology, CNRS, Strasbourg, 67084, France
    Science 284:1313-8. 1999
    ..In addition to its role in the early phase of defense, innate immunity in mammals appears to play a key role in stimulating the subsequent, clonal response of adaptive immunity...
  77. ncbi Interaction and specificity of Rel-related proteins in regulating Drosophila immunity gene expression
    Z S Han
    Program in Molecular Medicine, Department of Biochemistry and Molecular Biology, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
    J Biol Chem 274:21355-61. 1999
    ..Furthermore, the drosomycin and defensin expression is best induced by the Relish/Dif and the Relish/Dorsal heterodimers, respectively, ..
  78. ncbi Drosophila Toll is activated by Gram-positive bacteria through a circulating peptidoglycan recognition protein
    T Michel
    Institut de Biologie Moleculaire et Cellulaire, UPR 9022 du CNRS, 15 rue Rene Descartes, 67084 Strasbourg Cedex, France
    Nature 414:756-9. 2001
    ..Interestingly, seml does not affect Toll activation by fungal infection, indicating the existence of a distinct recognition system for fungi to activate the Toll pathway...
  79. ncbi Requirement for a peptidoglycan recognition protein (PGRP) in Relish activation and antibacterial immune responses in Drosophila
    Kwang Min Choe
    Molecular Biology Program, Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center, 1275 York Avenue, New York, NY 10021, USA
    Science 296:359-62. 2002
    ....
  80. pmc Overexpression of a pattern-recognition receptor, peptidoglycan-recognition protein-LE, activates imd/relish-mediated antibacterial defense and the prophenoloxidase cascade in Drosophila larvae
    Aya Takehana
    Graduate School of Pharmaceutical Sciences, Tohoku University, Sendai 980 8578, Japan
    Proc Natl Acad Sci U S A 99:13705-10. 2002
    ..Therefore, PGRP-LE acts as a pattern-recognition receptor to the diaminopimelic acid-type peptidoglycan and activates both the proteolytic cascade and intracellular signaling in Drosophila immunity...
  81. pmc The homeobox gene Caudal regulates constitutive local expression of antimicrobial peptide genes in Drosophila epithelia
    Ji Hwan Ryu
    Division of Molecular Life Science and Center for Cell Signaling Research, Ewha Womans University, Seoul, South Korea
    Mol Cell Biol 24:172-85. 2004
    ..modulator that is responsible for the constitutive local expression of antimicrobial peptides cecropin and drosomycin in a tissue-specific manner...
  82. ncbi Dual activation of the Drosophila toll pathway by two pattern recognition receptors
    Vanessa Gobert
    Unité Propre de Recherche 9022 du CNRS, Institut de Biologie Moleculaire et Cellulaire, 15 rue Rene Descartes, F67084 Strasbourg Cedex, France
    Science 302:2126-30. 2003
    ..The potential for a combination of distinct proteins to mediate detection of infectious nonself in the fly will refine the concept of pattern recognition in insects...
  83. ncbi Differential activation of the NF-kappaB-like factors Relish and Dif in Drosophila melanogaster by fungi and Gram-positive bacteria
    Marika Hedengren-Olcott
    Department of Zoology, Oregon State University, 220 Nash Hall, Corvallis, OR 97331, USA
    J Biol Chem 279:21121-7. 2004
    ....
  84. pmc Multimerization and interaction of Toll and Spätzle in Drosophila
    Xiaodi Hu
    Program in Molecular Medicin, University of Massachusetts Medical School, 373 Plantation Street, Worcester, MA 01605, USA
    Proc Natl Acad Sci U S A 101:9369-74. 2004
    ..a complex with Toll in transgenic fly extracts and stimulates the expression of a Toll-dependent immunity gene, drosomycin, in adult flies...
  85. pmc Functional evolution of the vertebrate Myb gene family: B-Myb, but neither A-Myb nor c-Myb, complements Drosophila Myb in hemocytes
    Colin J Davidson
    Department of Pathology, Stanford University School of Medicine, Stanford, California 94305, USA
    Genetics 169:215-29. 2005
    ..Finally, these experiments demonstrate the utility of genetic complementation in Drosophila to explore the functional evolution of duplicated genes in vertebrates...
  86. pmc Requirements of peptidoglycan structure that allow detection by the Drosophila Toll pathway
    Sergio R Filipe
    Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK
    EMBO Rep 6:327-33. 2005
    ..We suggest a model whereby GNBP1 is involved in the hydrolysis of Gram-positive peptidoglycan producing new glycan reducing ends, which are subsequently detected by PGRP-SA...
  87. pmc WntD is a feedback inhibitor of Dorsal/NF-kappaB in Drosophila development and immunity
    Michael D Gordon
    Department of Developmental Biology, Howard Hughes Medical Institute, Beckman Center and
    Nature 437:746-9. 2005
    ..Furthermore, the wntD mutant phenotype is suppressed by loss of zygotic dorsal. These results describe the first secreted feedback antagonist of Toll signalling, and demonstrate a novel Wnt activity in the fly...
  88. ncbi A Spätzle-processing enzyme required for toll signaling activation in Drosophila innate immunity
    In Hwan Jang
    Division of Molecular Life Science, Ewha Womans University, Seoul, 120 750, South Korea
    Dev Cell 10:45-55. 2006
    ....
  89. pmc Downregulation of the Drosophila immune response by peptidoglycan-recognition proteins SC1 and SC2
    Vincent Bischoff
    Institut de Biologie Moleculaire et Cellulaire, UPR 9022 du CNRS, Strasbourg, France
    PLoS Pathog 2:e14. 2006
    ..We further show that a strict control of IMD-pathway activation is essential to prevent bacteria-induced developmental defects and larval death...
  90. ncbi Natural selection drives extremely rapid evolution in antiviral RNAi genes
    Darren J Obbard
    Institute of Evolutionary Biology, University of Edinburgh, Kings Buildings, West Mains Road, Edinburgh EH9 3JT, United Kingdom
    Curr Biol 16:580-5. 2006
    ..This is a signature of host-pathogen arms races and implies that the ancient battle between RNA viruses and host antiviral RNAi genes is active and significant in shaping RNAi function...
  91. ncbi Trade-offs between longevity and pathogen resistance in Drosophila melanogaster are mediated by NFkappaB signaling
    Sergiy Libert
    Huffington Center on Aging, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA
    Aging Cell 5:533-43. 2006
    ..These phenotypes are dependent on the NFkappaB-related transcriptional factor, Relish, and they establish a mechanistic basis for a link between immunity, inflammation, and longevity...
  92. pmc Drosophila eiger mutants are sensitive to extracellular pathogens
    David S Schneider
    Department of Microbiology and Immunology, Stanford University, Stanford, California, United States of America
    PLoS Pathog 3:e41. 2007
    ..We propose that eiger activates the cellular immune response of the fly to aid clearance of extracellular pathogens. Intracellular pathogens, which can already defeat professional phagocytes, are unaffected by eiger...
  93. pmc Toll and IMD pathways synergistically activate an innate immune response in Drosophila melanogaster
    Takahiro Tanji
    Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Mol Cell Biol 27:4578-88. 2007
    ..ligand) together caused synergistic activation of representative target genes of the two pathways, including Drosomycin, Diptericin, and AttacinA...
  94. pmc Down-regulation of NF-kappaB target genes by the AP-1 and STAT complex during the innate immune response in Drosophila
    Lark Kyun Kim
    Department of Biochemistry, National Creative Research Initiative Center for Genome Regulation, Yonsei University, Seoul, Korea
    PLoS Biol 5:e238. 2007
    ..We conclude that an inhibitory effect of AP-1 and STAT on NF-kappaB is required for properly balanced immune responses and appears to be evolutionarily conserved...
  95. doi Infection-induced proteolysis of PGRP-LC controls the IMD activation and melanization cascades in Drosophila
    Rebecca L Schmidt
    Department of Biochemistry and Molecular Biology, Mayo Clinic College of Medicine, 200 First St SW, Rochester, MN 55905 USA
    FASEB J 22:918-29. 2008
    ....
  96. ncbi Pirk is a negative regulator of the Drosophila Imd pathway
    Anni Kleino
    Institute of Medical Technology, University of Tampere, Finland
    J Immunol 180:5413-22. 2008
    ..Furthermore, pirk-overexpressing flies were more susceptible to Gram-negative bacterial infection than wild-type flies. We conclude that Pirk is a negative regulator of the Imd pathway...
  97. pmc Sensing of 'danger signals' and pathogen-associated molecular patterns defines binary signaling pathways 'upstream' of Toll
    Laure El Chamy
    Institut de Biologie Moleculaire et Cellulaire, Centre National de la Recherche Scientifique Unité Propre de Recherche 9022, Universite Louis Pasteur, Strasbourg Cedex, France
    Nat Immunol 9:1165-70. 2008
    ..Hence, Persephone defines a parallel proteolytic cascade activated by 'danger signals' such as abnormal proteolytic activities...
  98. pmc Antiviral immunity in Drosophila requires systemic RNA interference spread
    Maria Carla Saleh
    Department of Microbiology and Immunology, University of California, San Francisco 94122 2280, USA
    Nature 458:346-50. 2009
    ..Thus, similar to protein-based immunity in vertebrates, the antiviral RNAi response in flies also relies on the systemic spread of a virus-specific immunity signal...
  99. pmc Elimination of plasmatocytes by targeted apoptosis reveals their role in multiple aspects of the Drosophila immune response
    Bernard Charroux
    Institut de Biologie du Developpement de Marseille Luminy, Unite Mixte de Recherche 6216, Centre National de la Recherche Scientifique, Université de la Méditerrannée Aix Marseille II, 13288 Marseille Cedex 9, France
    Proc Natl Acad Sci U S A 106:9797-802. 2009
    ..Finally, we show that plasmatocytes are involved in immune surveillance during pupal development, because they prevent bacterial infection that causes pupal lethality...
  100. pmc Genetic screen identifies serpin5 as a regulator of the toll pathway and CHMP2B toxicity associated with frontotemporal dementia
    S Tariq Ahmad
    Gladstone Institute of Neurological Disease, San Francisco, CA 94158, USA
    Proc Natl Acad Sci U S A 106:12168-73. 2009
    ..This fly model will be useful to further dissect genetic pathways that are potentially relevant to the pathogenesis and treatment of FTD...
  101. pmc A single modular serine protease integrates signals from pattern-recognition receptors upstream of the Drosophila Toll pathway
    Nicolas Buchon
    Global Health Institute, Ecole Polytechnique Federale de Lausanne, 1015 Lausanne, Switzerland
    Proc Natl Acad Sci U S A 106:12442-7. 2009
    ..It also reveals the conserved role of modular serine proteases in the activation of insect immune reactions...

Research Grants3

  1. The Role of Peroxiredoxins in Immunity and Aging
    SVETLANA RADYUK; Fiscal Year: 2006
    ..This will set the stage for a more thorough investigation of the molecular underpinnings of innate immunity during aging. ..
  2. Using Drosphila Macrophages to Study Innate Immunity
    David Schneider; Fiscal Year: 2007
    ..These experiments will provide tools to analyze a collection of Drosophila mutants isolated based upon a phenotype that is likely to involve the cellular immune response. ..
  3. REGULATION OF IMMUNE RESPONSE AND CELL GROWTH
    DEBORAH KIMBRELL; Fiscal Year: 2001
    ..abstract_text> ..

Patents1

  1. CHIMERIC GENE CODING FOR DROSOMICINE, VECTOR CONTAINING IT AND PRODUCTION OF TRANSGENIC PLANTS RESISTANT TO DISEASES
    Patent Number: WO9902717-A1; Date:1999-01-21