cid

Summary

Gene Symbol: cid
Description: centromere identifier
Alias: BcDNA:RE21270, CENH3, CENP-A, CENP-A/CID, CENP-A/Cid, CENPA, CG13329, CID, CID/CENP-A, CenH3, CenH3[CID], CenH3[Cid], Cenp-A, CenpA, CenpA/CID, Cid, Cid[Mel], Dmel\CG13329, cenH3, cenpA, CG13329 gene product from transcript CG13329-RA, CG13329-PA, centromere identifier protein, centromere protein A, centromeric histone H3, cid-PA
Species: fruit fly

Top Publications

  1. ncbi Incorporation of Drosophila CID/CENP-A and CENP-C into centromeres during early embryonic anaphase
    Melina Schuh
    Bayreuth Center for Molecular Biosciences BZMB, Department of Genetics, University of Bayreuth, 95440 Bayreuth, Germany
    Curr Biol 17:237-43. 2007
  2. pmc Drosophila Mis12 complex acts as a single functional unit essential for anaphase chromosome movement and a robust spindle assembly checkpoint
    Zsolt Venkei
    Department of Genetics, University of Cambridge, Cambridge CB2 3EH, United Kingdom
    Genetics 187:131-40. 2011
  3. pmc Spindle checkpoint-independent inhibition of mitotic chromosome segregation by Drosophila Mps1
    Friederike Althoff
    Institute of Molecular Life Sciences, University of Zurich, 8057 Zurich, Switzerland
    Mol Biol Cell 23:2275-91. 2012
  4. ncbi Regulation of meiotic cohesion and chromosome core morphogenesis during pachytene in Drosophila oocytes
    Radhika S Khetani
    Department of Biological Sciences, Dartmouth College, Hanover, NH 03755, USA
    J Cell Sci 120:3123-37. 2007
  5. ncbi The Drosophila maternal gene sésame is required for sperm chromatin remodeling at fertilization
    B Loppin
    Centre de Genetique Moleculaire et Cellulaire, CNRS UMR 5534, Universite Claude Bernard Lyon, Villeurbanne, France
    Chromosoma 110:430-40. 2001
  6. ncbi Depletion of Drad21/Scc1 in Drosophila cells leads to instability of the cohesin complex and disruption of mitotic progression
    Sharron Vass
    Wellcome Trust Centre for Cell Biology, Institute of Cell and Molecular Biology, University of Edinburgh, Michael Swann Building, King s Buildings, Mayfield Road, Edinburgh EH9 3JR, Scotland, United Kingdom
    Curr Biol 13:208-18. 2003
  7. ncbi The spindle-associated transmembrane protein Axs identifies a membranous structure ensheathing the meiotic spindle
    Joseph Kramer
    Stowers Institute for Medical Research, Kansas City, MO 64110, USA
    Nat Cell Biol 5:261-3. 2003
  8. ncbi Condensin-dependent localisation of topoisomerase II to an axial chromosomal structure is required for sister chromatid resolution during mitosis
    Paula A Coelho
    Instituto de Biologia Molecular e Celular, Universidade do Porto, Rua do Campo Alegre 823, 4150 180 Porto, Portugal
    J Cell Sci 116:4763-76. 2003
  9. ncbi Different spindle checkpoint proteins monitor microtubule attachment and tension at kinetochores in Drosophila cells
    Elsa Logarinho
    Instituto de Biologia Molecular e Celular, Universidade do Porto, Rua do Campo Alegre 823, 4150 180 Porto, Portugal
    J Cell Sci 117:1757-71. 2004
  10. pmc Cell cycle-dependent dynamics and regulation of mitotic kinesins in Drosophila S2 cells
    Gohta Goshima
    The Howard Hughes Medical Institute and the Department of Cellular and Molecular Pharmacology, University of California San Francisco, San Francisco, CA 94107, USA
    Mol Biol Cell 16:3896-907. 2005

Scientific Experts

Detail Information

Publications108 found, 100 shown here

  1. ncbi Incorporation of Drosophila CID/CENP-A and CENP-C into centromeres during early embryonic anaphase
    Melina Schuh
    Bayreuth Center for Molecular Biosciences BZMB, Department of Genetics, University of Bayreuth, 95440 Bayreuth, Germany
    Curr Biol 17:237-43. 2007
    ..Thus, our findings uncover a strikingly dynamic behavior of centromere components in anaphase...
  2. pmc Drosophila Mis12 complex acts as a single functional unit essential for anaphase chromosome movement and a robust spindle assembly checkpoint
    Zsolt Venkei
    Department of Genetics, University of Cambridge, Cambridge CB2 3EH, United Kingdom
    Genetics 187:131-40. 2011
    ..Importantly our results indicate that core structural elements of the inner plate of the kinetochore have a greater contribution to faithful chromosome segregation in anaphase than in earlier stages of mitosis...
  3. pmc Spindle checkpoint-independent inhibition of mitotic chromosome segregation by Drosophila Mps1
    Friederike Althoff
    Institute of Molecular Life Sciences, University of Zurich, 8057 Zurich, Switzerland
    Mol Biol Cell 23:2275-91. 2012
    ..These findings further expand Mps1's significance for faithful mitotic chromosome segregation and emphasize the importance of its careful regulation...
  4. ncbi Regulation of meiotic cohesion and chromosome core morphogenesis during pachytene in Drosophila oocytes
    Radhika S Khetani
    Department of Biological Sciences, Dartmouth College, Hanover, NH 03755, USA
    J Cell Sci 120:3123-37. 2007
    ..We present a model for how chromosome cores are assembled during Drosophila meiosis and the role of ORD in meiotic cohesion, chromosome core maintenance and homologous recombination...
  5. ncbi The Drosophila maternal gene sésame is required for sperm chromatin remodeling at fertilization
    B Loppin
    Centre de Genetique Moleculaire et Cellulaire, CNRS UMR 5534, Universite Claude Bernard Lyon, Villeurbanne, France
    Chromosoma 110:430-40. 2001
    ..The abnormal chromatin organization of the male pronucleus does not prevent the formation of a male pronuclear envelope, which breaks down and reassembles in synchrony with maternally derived nuclei present in the same cytoplasm...
  6. ncbi Depletion of Drad21/Scc1 in Drosophila cells leads to instability of the cohesin complex and disruption of mitotic progression
    Sharron Vass
    Wellcome Trust Centre for Cell Biology, Institute of Cell and Molecular Biology, University of Edinburgh, Michael Swann Building, King s Buildings, Mayfield Road, Edinburgh EH9 3JR, Scotland, United Kingdom
    Curr Biol 13:208-18. 2003
    ..In this study, we examine the consequences of depleting Drad21 and SA, two non-SMC subunits of the cohesin complex, by dsRNA-mediated interference in Drosophila cultured cells...
  7. ncbi The spindle-associated transmembrane protein Axs identifies a membranous structure ensheathing the meiotic spindle
    Joseph Kramer
    Stowers Institute for Medical Research, Kansas City, MO 64110, USA
    Nat Cell Biol 5:261-3. 2003
    ..In both meiotic and mitotic cells, Axs is recruited to the microtubules of assembling spindles. We propose that Axs and the sheath represent novel mediators of meiotic spindle assembly and chromosome segregation...
  8. ncbi Condensin-dependent localisation of topoisomerase II to an axial chromosomal structure is required for sister chromatid resolution during mitosis
    Paula A Coelho
    Instituto de Biologia Molecular e Celular, Universidade do Porto, Rua do Campo Alegre 823, 4150 180 Porto, Portugal
    J Cell Sci 116:4763-76. 2003
    ..Our results suggest that condensin is required so that an axial chromatid structure can be organised where topoisomerase II can effectively promote sister chromatid resolution...
  9. ncbi Different spindle checkpoint proteins monitor microtubule attachment and tension at kinetochores in Drosophila cells
    Elsa Logarinho
    Instituto de Biologia Molecular e Celular, Universidade do Porto, Rua do Campo Alegre 823, 4150 180 Porto, Portugal
    J Cell Sci 117:1757-71. 2004
    ....
  10. pmc Cell cycle-dependent dynamics and regulation of mitotic kinesins in Drosophila S2 cells
    Gohta Goshima
    The Howard Hughes Medical Institute and the Department of Cellular and Molecular Pharmacology, University of California San Francisco, San Francisco, CA 94107, USA
    Mol Biol Cell 16:3896-907. 2005
    ..These results reveal a diverse spectrum of regulatory mechanisms for controlling the localization and function of five mitotic kinesins at different stages of the cell cycle...
  11. pmc Live imaging of Drosophila brain neuroblasts reveals a role for Lis1/dynactin in spindle assembly and mitotic checkpoint control
    Karsten H Siller
    Howard Hughes Medical Institute, Eugene, OR 97403, USA
    Mol Biol Cell 16:5127-40. 2005
    ..We conclude that Lis1/dynactin act together to regulate multiple, independent functions in mitotic cells, including spindle formation and cell cycle checkpoint release...
  12. ncbi Identification of two proteins required for conjunction and regular segregation of achiasmate homologs in Drosophila male meiosis
    Sharon E Thomas
    Department of Biochemistry, Cellular and Molecular Biology, University of Tennessee, Knoxville, Tennessee 37996, USA
    Cell 123:555-68. 2005
    ..SNM and MNM do not colocalize with SMC1, suggesting that the homolog conjunction mechanism is independent of cohesin...
  13. pmc Chaperone-mediated assembly of centromeric chromatin in vitro
    Takehito Furuyama
    Fred Hutchinson Cancer Research Center and Howard Hughes Medical Institute, 1100 Fairview Avenue North, Seattle, WA 98109, USA
    Proc Natl Acad Sci U S A 103:6172-7. 2006
    ..centromeres are universally marked by the presence of a centromeric histone variant, centromeric histone 3 (CenH3), which replaces canonical histone H3 in centromeric nucleosomes...
  14. ncbi Antagonistic activities of Klp10A and Orbit regulate spindle length, bipolarity and function in vivo
    Joseph E Laycock
    Cancer Research UK Cell Cycle Genetics Group, University of Cambridge, Department of Genetics
    J Cell Sci 119:2354-61. 2006
    ..We conclude that Klp10A exclusively antagonises Orbit in the regulation of bipolar spindle formation and maintenance...
  15. pmc Drosophila CENP-A mutations cause a BubR1-dependent early mitotic delay without normal localization of kinetochore components
    Michael D Blower
    Department of Molecular and Cell Biology, University of California Berkeley, Berkeley, California, USA
    PLoS Genet 2:e110. 2006
    ..Here we show that cells in Drosophila embryos homozygous for null mutations in CENP-A (CID) display an early mitotic delay...
  16. pmc Mad2-independent spindle assembly checkpoint activation and controlled metaphase-anaphase transition in Drosophila S2 cells
    Bernardo Orr
    Instituto de Biologia Molecular e Celular, 4150 180 Porto, Portugal
    Mol Biol Cell 18:850-63. 2007
    ....
  17. pmc Kinesin-5-dependent poleward flux and spindle length control in Drosophila embryo mitosis
    Ingrid Brust-Mascher
    Department of Molecular and Cellular Biology, University of California, Davis, CA 95616, USA
    Mol Biol Cell 20:1749-62. 2009
    ..Finally, KLP61F activity contributes to normal chromosome congression, kinetochore spacing, and anaphase A rates. Thus, a KLP61F-driven sliding filament mechanism contributes to multiple aspects of mitosis in this system...
  18. pmc Heterochromatic deposition of centromeric histone H3-like proteins
    S Henikoff
    Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue North, Seattle, WA 98109 1024, USA
    Proc Natl Acad Sci U S A 97:716-21. 2000
    ..We describe a Drosophila H3-like protein, Cid (for centromere identifier) that localizes exclusively to fly centromeres...
  19. pmc Adaptive evolution of Cid, a centromere-specific histone in Drosophila
    H S Malik
    Howard Hughes Medical Institute, Seattle, Washington 98109, USA
    Genetics 157:1293-8. 2001
    ..Histone H3-like proteins, including Cid in Drosophila melanogaster, are a unique chromatin component of centromeres...
  20. pmc The hinge and chromo shadow domain impart distinct targeting of HP1-like proteins
    J F Smothers
    Howard Hughes Medical Institute, Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109 1024, USA
    Mol Cell Biol 21:2555-69. 2001
    ..We propose that these targeting features allow different HP1 complexes to be distinctly sequestered in organisms that harbor multiple HP1-like proteins...
  21. pmc Centromeres are specialized replication domains in heterochromatin
    K Ahmad
    Howard Hughes Medical Institute, and Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA
    J Cell Biol 153:101-10. 2001
    ..These domains do not appear to assemble conventional H3-containing nucleosomes, and deposition of the Cid centromeric H3-like variant proceeds by a replication-independent pathway...
  22. pmc Essential roles of Drosophila inner centromere protein (INCENP) and aurora B in histone H3 phosphorylation, metaphase chromosome alignment, kinetochore disjunction, and chromosome segregation
    R R Adams
    Wellcome Center for Cell Biology, Institute for Cell and Molecular Biology, University of Edinburgh, Mayfield Road, Edinburgh EH9 3JR, Scotland, United Kingdom
    J Cell Biol 153:865-80. 2001
    ..These experiments reveal that INCENP is required for aurora B kinase function and confirm that the chromosomal passengers have essential roles in mitosis...
  23. pmc The role of Drosophila CID in kinetochore formation, cell-cycle progression and heterochromatin interactions
    M D Blower
    Molecular and Cell Biology Laboratories, The Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, California 92037, USA
    Nat Cell Biol 3:730-9. 2001
    ..Here we show that CID, the Drosophila homologue of the CENP-A centromere-specific H3-like proteins, colocalizes with molecular-..
  24. pmc Centromere identity in Drosophila is not determined in vivo by replication timing
    B Sullivan
    Molecular and Cell Biology Laboratory, The Salk Institute for Biological Studies, La Jolla, CA 92037, USA
    J Cell Biol 154:683-90. 2001
    ..Two different nucleotide incorporation methods were used to evaluate replication timing of chromatin containing CID, a Drosophila homologue of CENP-A...
  25. pmc Conserved organization of centromeric chromatin in flies and humans
    Michael D Blower
    Molecular and Cell Biology Laboratory, The Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, CA 92037, USA
    Dev Cell 2:319-30. 2002
    ..We show that Drosophila CID and human CENP-A appear at metaphase as a three-dimensional structure that lacks histone H3...
  26. ncbi Centromeres and variant histones: what, where, when and why?
    M Mitchell Smith
    Department of Microbiology and University of Virginia Cancer Center, Jordan Building, Room 7223, University of Virginia, 1300 Jefferson Park Avenue, Charlottesville, VA 22908, USA
    Curr Opin Cell Biol 14:279-85. 2002
    ..Finally, a new report provides a glimpse into the potential regulation of CENP-A through specific post-translational phosphorylation, suggesting a broad level of control through histone tail modifications...
  27. ncbi The histone variant H3.3 marks active chromatin by replication-independent nucleosome assembly
    Kami Ahmad
    Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue North, Seattle, WA 98109, USA
    Mol Cell 9:1191-200. 2002
    ..RI substitution of H3.3 provides a mechanism for the immediate activation of genes that are silenced by histone modification. Inheritance of newly deposited nucleosomes may then mark sites as active loci...
  28. pmc Histone H3 variants specify modes of chromatin assembly
    Kami Ahmad
    Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue North, A1 162, Seattle, WA 98109, USA
    Proc Natl Acad Sci U S A 99:16477-84. 2002
    ..H3 packages the bulk genome, H3.3 marks active chromatin and may be essential for gene regulation, and Cid is the characteristic structural component of centromeric chromatin...
  29. ncbi The dynamics of homologous chromosome pairing during male Drosophila meiosis
    Julio Vazquez
    Department of Biochemistry and Biophysics, University of California, San Francisco, 94143, USA
    Curr Biol 12:1473-83. 2002
    ..Due to limitations in available cytological techniques, the early stages of homologous chromosome pairing in male Drosophila have not been observed, and the mechanisms involved are poorly understood...
  30. pmc Centromere targeting element within the histone fold domain of Cid
    Danielle Vermaak
    Howard Hughes Medical Institute, Basic Sciences Division, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA
    Mol Cell Biol 22:7553-61. 2002
    ..Drosophila sp. centromeric nucleosomes contain the Cid H3-like protein...
  31. pmc Microtubule flux and sliding in mitotic spindles of Drosophila embryos
    Ingrid Brust-Mascher
    Center for Genetics and Development and Section of Molecular and Cellular Biology, University of California, Davis, California 95616, USA
    Mol Biol Cell 13:3967-75. 2002
    ....
  32. ncbi Two putative acetyltransferases, san and deco, are required for establishing sister chromatid cohesion in Drosophila
    Byron C Williams
    Department of Molecular Biology and Genetics, 427 Biotechnology Building, Cornell University, Ithaca, NY 14853, USA
    Curr Biol 13:2025-36. 2003
    ..However, many aspects of the mechanisms that establish and maintain cohesion during mitosis remain unclear...
  33. ncbi Two mitotic kinesins cooperate to drive sister chromatid separation during anaphase
    Gregory C Rogers
    Department of Physiology and Biophysics, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    Nature 427:364-70. 2004
    ..The other, KLP10A, is required to depolymerize microtubules at their pole-associated minus ends, thereby moving chromatids by means of poleward flux...
  34. pmc The cohesion protein ORD is required for homologue bias during meiotic recombination
    Hayley A Webber
    Dept of Biological Sciences, Dartmouth College, 6044 Gilman, Hanover, NH 03755, USA
    J Cell Biol 164:819-29. 2004
    ..We conclude that ORD activity suppresses sister chromatid exchange and stimulates inter-homologue crossovers, thereby promoting homologue bias during meiotic recombination in Drosophila...
  35. ncbi Homologous pairing and chromosome dynamics in meiosis and mitosis
    Bruce D McKee
    Department of Biochemistry and Cellular and Molecular Biology and Genome Sciences and Technology Program, University of Tennessee, Knoxville, M407 Walters Life Sciences Building, Knoxville, TN 37996 0840, USA
    Biochim Biophys Acta 1677:165-80. 2004
    ..I cite evidence that may point to a relationship between matrix attachment sites and homologous pairing sites...
  36. pmc Centromeric chromatin exhibits a histone modification pattern that is distinct from both euchromatin and heterochromatin
    Beth A Sullivan
    Molecular and Cell Biology Laboratory, The Salk Institute, 10010 N Torrey Pines Road, La Jolla, California 92037, USA
    Nat Struct Mol Biol 11:1076-83. 2004
    ..We speculate that this distinct modification pattern contributes to the unique domain organization and three-dimensional structure of centromeric regions, and/or to the epigenetic information that determines centromere identity...
  37. ncbi The mitotic arrest in response to hypoxia and of polar bodies during early embryogenesis requires Drosophila Mps1
    Matthias G Fischer
    BZMB, Department of Genetics, University of Bayreuth, 94440 Bayreuth, Germany
    Curr Biol 14:2019-24. 2004
    ..Finally, we show that Mps1 and the mitotic spindle checkpoint are responsible for the developmental cell cycle arrest of the three haploid products of female meiosis that are not used as the female pronucleus...
  38. ncbi The Drosophila melanogaster condensin subunit Cap-G interacts with the centromere-specific histone H3 variant CID
    Hubert Jäger
    Lehrstuhl fur Genetik, University of Bayreuth, 95440 Bayreuth, Germany
    Chromosoma 113:350-61. 2005
    ..We chose a genetic approach to identify interactors of the Drosophila CENP-A homolog CID. Overexpression of cid in the proliferating eye imaginal disk results in a rough eye phenotype, which is dependent ..
  39. pmc Genetic interactions of separase regulatory subunits reveal the diverged Drosophila Cenp-C homolog
    Sebastian Heeger
    Department of Genetics, BZMB, University of Bayreuth, 95440 Bayreuth, Germany
    Genes Dev 19:2041-53. 2005
    ....
  40. ncbi Maternal expression of the checkpoint protein BubR1 is required for synchrony of syncytial nuclear divisions and polar body arrest in Drosophila melanogaster
    Daniel Pérez-Mongiovi
    Instituto de Biologia Molecular e Celular, Universidade do Porto, Rua do Campo Alegre 823, 4150 180 Porto, Portugal
    Development 132:4509-20. 2005
    ..Our results suggest that BubR1 is required for normal synchrony and progression of syncytial nuclei through mitosis and to maintain the mitotic arrest of the polar body chromosomes after completion of meiosis...
  41. pmc The condensin I subunit Barren/CAP-H is essential for the structural integrity of centromeric heterochromatin during mitosis
    Raquel A Oliveira
    Instituto de Biologia Molecular e Celular, Universidade do Porto, Portugal
    Mol Cell Biol 25:8971-84. 2005
    ..Taken together, our data suggest that the condensin I complex is required not only to promote sister chromatid resolution but also to maintain the structural integrity of centromeric heterochromatin during mitosis...
  42. pmc The conserved kinase NHK-1 is essential for mitotic progression and unifying acentrosomal meiotic spindles in Drosophila melanogaster
    C Fiona Cullen
    Wellcome Trust Centre for Cell Biology, Institute of Cell Biology, School of Biological Sciences, The University of Edinburgh, Edinburgh EH9 3JR, Scotland, UK
    J Cell Biol 171:593-602. 2005
    ..NHK-1 itself is phosphorylated in mitosis and female meiosis, suggesting that this kinase is part of the regulatory system coordinating progression of mitosis and meiosis...
  43. ncbi Chromatin assembly: a basic recipe with various flavours
    Sophie E Polo
    Laboratory of Nuclear Dynamics and Genome Plasticity, UMR 218 CNRS Institut Curie, 26 Rue d Ulm, 75248 Paris Cedex 5, France
    Curr Opin Genet Dev 16:104-11. 2006
    ..New data highlight the importance of histone dimers as deposition entities for de novo nucleosome assembly and identify dedicated machineries involved in histone variant deposition...
  44. ncbi The emergence of regulated histone proteolysis
    Akash Gunjan
    Florida State University College of Medicine, Department of Biomedical Sciences, 1115 West Call Street, Tallahassee, FL 32309 4300, USA
    Curr Opin Genet Dev 16:112-8. 2006
    ..Recent discoveries have highlighted the importance of regulated histone proteolysis in preventing both the accumulation of excess histones and the mis-incorporation of histone variants at inappropriate loci...
  45. pmc Mislocalization of the Drosophila centromere-specific histone CID promotes formation of functional ectopic kinetochores
    Patrick Heun
    Department of Genome Biology, Lawrence Berkeley National Lab, One Cyclotron Road, Berkeley, California 94720, USA
    Dev Cell 10:303-15. 2006
    The centromere-specific histone variant CENP-A (CID in Drosophila) is a structural and functional foundation for kinetochore formation and chromosome segregation...
  46. pmc The ultrastructure of the kinetochore and kinetochore fiber in Drosophila somatic cells
    Helder Maiato
    Institute for Molecular and Cell Biology, Rua do Campo Alegre 823, 4150 180, Porto, Portugal
    Chromosoma 115:469-80. 2006
    ..Our structural observations provide important data for assessing the results of RNAi studies of mitosis, as well as for the development of mathematical modelling and computer simulation studies in Drosophila and related organisms...
  47. pmc Proteolysis restricts localization of CID, the centromere-specific histone H3 variant of Drosophila, to centromeres
    Olga Moreno-Moreno
    Institute of Molecular Biology of Barcelona IBMB, CSIC, Parc Cientific de Barcelona, 08028 Barcelona, Spain
    Nucleic Acids Res 34:6247-55. 2006
    ..in Drosophila Kc cells, we show that proteasome-mediated degradation restricts localization of Drosophila CENP-A (CID) to centromeres by eliminating mislocalized CID as well as by regulating available CID levels...
  48. ncbi Condensin I binds chromatin early in prophase and displays a highly dynamic association with Drosophila mitotic chromosomes
    Raquel A Oliveira
    Instituto de Biologia Molecular e Celular, Universidade do Porto, Rua do Campo Alegre 823, 4150 180 Porto, Portugal
    Chromosoma 116:259-74. 2007
    ..Furthermore, the rapid turnover of Barren-EGFP indicates that the mechanism by which condensin I promotes mitotic chromosome organization is inconsistent with a static scaffold model...
  49. pmc Spatial organization of a ubiquitous eukaryotic kinetochore protein network in Drosophila chromosomes
    Ralf B Schittenhelm
    Department of Genetics, University of Bayreuth, Universitaetsstr 30, 95447 Bayreuth, Germany
    Chromosoma 116:385-402. 2007
    ..Thereby, Cenp-A/Cid, Cenp-C, Mis12 and the Ndc80 complex were mapped along the inter sister kinetochore axis with a resolution below 10 ..
  50. pmc Poleward tubulin flux in spindles: regulation and function in mitotic cells
    Daniel W Buster
    Department of Physiology and Biophysics, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Mol Biol Cell 18:3094-104. 2007
    ....
  51. pmc Spindly, a novel protein essential for silencing the spindle assembly checkpoint, recruits dynein to the kinetochore
    Eric R Griffis
    Howard Hughes Medical Institute, University of California, San Francisco, CA 94158, USA
    J Cell Biol 177:1005-15. 2007
    ..However, dynein's nonkinetochore functions are unaffected by Spindly depletion. Our findings indicate that Spindly is a novel regulator of mitotic dynein, functioning specifically to target dynein to kinetochores...
  52. pmc The multiple roles of mps1 in Drosophila female meiosis
    William D Gilliland
    Stowers Institute for Medical Research, Kansas City, Missouri, United States of America
    PLoS Genet 3:e113. 2007
    ..These filaments polymerize during early germinal vesicle breakdown, perhaps as a means of storing excess outer kinetochore kinases during early embryonic development...
  53. ncbi Rapid effects of acute anoxia on spindle kinetochore interactions activate the mitotic spindle checkpoint
    Rahul Pandey
    Department of Genetics, BZMB, University of Bayreuth, 95440 Bayreuth, Germany
    J Cell Sci 120:2807-18. 2007
    ..Although involving adenosine triphosphate (ATP)-consuming kinases, the spindle checkpoint can therefore be activated by spindle damage in response to acute anoxia and protect against aneuploidies...
  54. pmc Tetrameric structure of centromeric nucleosomes in interphase Drosophila cells
    Yamini Dalal
    Fred Hutchinson Cancer Research Center, Seattle, Washington, United States of America
    PLoS Biol 5:e218. 2007
    ..segregation of chromosomes at mitosis, are epigenetically maintained by a centromere-specific histone H3 variant (CenH3). However, the mechanistic basis for centromere maintenance is unknown...
  55. pmc Structure, dynamics, and evolution of centromeric nucleosomes
    Yamini Dalal
    Basic Sciences Division and Howard Hughes Medical Institute, Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue North, Seattle, WA 98109, USA
    Proc Natl Acad Sci U S A 104:15974-81. 2007
    ..which differs from the conventional nucleosome by the presence of a centromere-specific histone variant (CenH3) in place of canonical H3...
  56. pmc Functional links between Drosophila Nipped-B and cohesin in somatic and meiotic cells
    Maria Gause
    Edward A Doisy Department of Biochemistry and Molecular Biology, Saint Louis University School of Medicine, Saint Louis, MO 63104, USA
    Chromosoma 117:51-66. 2008
    ..These results support the idea that direct regulation of cohesin function underlies the diverse functions of Nipped-B and its orthologs...
  57. pmc Cell-type-specific TEV protease cleavage reveals cohesin functions in Drosophila neurons
    Andrea Pauli
    Department of Biochemistry, University of Oxford, Oxford OX1 3QU, UK
    Dev Cell 14:239-51. 2008
    ..These data demonstrate essential roles for cohesin in nondividing cells and also introduce a powerful tool by which to investigate protein function in metazoa...
  58. pmc Differential requirements of a mitotic acetyltransferase in somatic and germ line cells
    Ana Pimenta-Marques
    Instituto Gulbenkian de Ciencia, Rua da Quinta Grande, N 6, 2781 901 Oeiras, Portugal
    Dev Biol 323:197-206. 2008
    ..Surprisingly, during oogenesis we cannot detect mitotic defects in germ line cells mutant for san. We hypothesize the female germ line stem cells have differential requirements for mitotic sister chromatid cohesion...
  59. pmc Sgt1, a co-chaperone of Hsp90 stabilizes Polo and is required for centrosome organization
    Torcato Martins
    IBMC Instituto de Biologia Molecular e Celular, Universidade do Porto, Porto, Portugal
    EMBO J 28:234-47. 2009
    ..Taken together, these findings suggest that Sgt1 is involved in the stabilization of Polo allowing normal centrosome maturation, entry and progression though mitosis...
  60. pmc Intrakinetochore stretch is associated with changes in kinetochore phosphorylation and spindle assembly checkpoint activity
    Thomas J Maresca
    Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    J Cell Biol 184:373-81. 2009
    ..This intrakinetochore stretch is largely independent of a 30-fold variation in centromere stretch. Furthermore, loss of intrakinetochore stretch is associated with enhancement of 3F3/2 phosphorylation and SAC activation...
  61. pmc Synchronizing chromosome segregation by flux-dependent force equalization at kinetochores
    Irina Matos
    Instituto de Biologia Molecular e Celular, Universidade do Porto, Porto, Portugal
    J Cell Biol 186:11-26. 2009
    ..We propose that flux-dependent equalization of kinetochore tensions ensures a timely and uniform maturation of kinetochore-microtubule interfaces necessary for error-free and coordinated segregation of chromosomes in anaphase...
  62. pmc Intrakinetochore localization and essential functional domains of Drosophila Spc105
    Ralf B Schittenhelm
    Institute of Zoology, University of Zurich, Zurich, Switzerland
    EMBO J 28:2374-86. 2009
    ..Spc105 seems to provide a platform within the outer kinetochore allowing independent assembly of various kinetochore components...
  63. pmc Centromeric nucleosomes induce positive DNA supercoils
    Takehito Furuyama
    Howard Hughes Medical Institute, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA
    Cell 138:104-13. 2009
    ..Centromere identity and inheritance require the assembly of nucleosomes containing the CenH3 histone variant in place of canonical H3...
  64. pmc The Drosophila HP1 homolog Rhino is required for transposon silencing and piRNA production by dual-strand clusters
    Carla Klattenhoff
    University of Massachusetts Medical School, Worcester, MA 01605, USA
    Cell 138:1137-49. 2009
    ..Rhino thus appears to promote transcription of dual-strand clusters, leading to production of piRNAs that drive the ping-pong amplification cycle...
  65. pmc Transcriptional and developmental functions of the H3.3 histone variant in Drosophila
    Akiko Sakai
    Department of BCMP, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA
    Curr Biol 19:1816-20. 2009
    ..Our results imply that the H3.3 variant plays an essential role in chromatin transitions in the male germline...
  66. pmc Cohesin cleavage and Cdk inhibition trigger formation of daughter nuclei
    Raquel A Oliveira
    Department of Biochemistry, University of Oxford, South Parks Road, Oxford, OX1 3QU, UK
    Nat Cell Biol 12:185-92. 2010
    ..We conclude that only two key events, opening of cohesin rings and downregulation of Cdk1, are sufficient to drive proper segregation of chromosomes in anaphase...
  67. pmc BubR1- and Polo-coated DNA tethers facilitate poleward segregation of acentric chromatids
    Anne Royou
    Department of Molecular, Cell, and Developmental Biology, University of California, Santa Cruz, Santa Cruz, CA 95064, USA
    Cell 140:235-45. 2010
    ..We propose that BubR1 and Polo facilitate the accurate segregation of acentric chromatids by maintaining the integrity of the tethers that connect acentric chromosomes to their centric partners...
  68. pmc SOLO: a meiotic protein required for centromere cohesion, coorientation, and SMC1 localization in Drosophila melanogaster
    Rihui Yan
    Department of Biochemistry, Cellular, and Molecular Biology, University of Tennessee, Knoxville, TN 37996, USA
    J Cell Biol 188:335-49. 2010
    ..The solo mutant phenotypes and the localization patterns of SOLO and SMC1 indicate that they function together to maintain sister chromatid cohesion in Drosophila meiosis...
  69. pmc Coupling between microtubule sliding, plus-end growth and spindle length revealed by kinesin-8 depletion
    Haifeng Wang
    Department of Molecular and Cell Biology, One Shields Avenue, University of California Davis, Davis, California 95616, USA
    Cytoskeleton (Hoboken) 67:715-28. 2010
    ....
  70. doi Drosophila CENP-C is essential for centromere identity
    Bernardo Orr
    Instituto de Biologia Molecular e Celular, Universidade do Porto, Rua do Campo Alegre 823, 4150 180 Porto, Portugal
    Chromosoma 120:83-96. 2011
    ..specialized chromosomal domains that direct mitotic kinetochore assembly and are defined by the presence of CENP-A (CID in Drosophila) and CENP-C...
  71. doi Detrimental incorporation of excess Cenp-A/Cid and Cenp-C into Drosophila centromeres is prevented by limiting amounts of the bridging factor Cal1
    Ralf B Schittenhelm
    Institute of Molecular Life Sciences, University of Zurich, CH 8057 Zurich, Switzerland
    J Cell Sci 123:3768-79. 2010
    ..eukaryotes depends critically on the faithful incorporation of a centromere-specific histone H3 variant encoded by CENPA in humans and cid in Drosophila...
  72. pmc A single mutation results in diploid gamete formation and parthenogenesis in a Drosophila yemanuclein-alpha meiosis I defective mutant
    Régis E Meyer
    Institut de Genetique Humaine IGH, Unité Propre de Recherche 1142, Centre National de la Recherche Scientifique CNRS, 141 rue de la Cardonille, 34396 Montpellier Cedex 5, France
    BMC Genet 11:104. 2010
    ..Therefore the underlying mechanisms must have been evolutionary linked and there is a need for evidence that could support such a model...
  73. doi Specialization of a Drosophila capping protein essential for the protection of sperm telomeres
    Raphaelle Dubruille
    Centre de Genetique Moleculaire et Cellulaire, UMR5534, France
    Curr Biol 20:2090-9. 2010
    ..We previously showed that the hiphop gene was duplicated before the radiation of the melanogaster subgroup of species, giving birth to K81, a unique paternal effect gene specifically expressed in the male germline...
  74. pmc Assembly of Drosophila centromeric chromatin proteins during mitosis
    Barbara G Mellone
    Department of Molecular and Cell Biology, University of Connecticut, Storrs, Connecticut, United States of America
    PLoS Genet 7:e1002068. 2011
    ..We analyzed the cell-cycle timing of centromeric chromatin assembly in Drosophila, which contains the H3 variant CID (CENP-A in humans), as well as CENP-C and CAL1, which are required for CID localization...
  75. doi The F box protein partner of paired regulates stability of Drosophila centromeric histone H3, CenH3(CID)
    Olga Moreno-Moreno
    Institute of Molecular Biology of Barcelona, CSIC, Barcelona, Spain
    Curr Biol 21:1488-93. 2011
    Centromere identity and function is determined by the specific localization of CenH3 (reviewed in [1-7])...
  76. doi A pathway for synapsis initiation during zygotene in Drosophila oocytes
    Nikhila S Tanneti
    Waksman Institute and Department of Genetics, Rutgers, The State University of New Jersey, Piscataway, NJ 08854 8020, USA
    Curr Biol 21:1852-7. 2011
    ..Furthermore, the early euchromatic SC initiation events at mid-zygotene may be required for DSBs to be repaired as crossovers...
  77. doi Drosophila CENH3 is sufficient for centromere formation
    María José Mendiburo
    Max Planck Institute of Immunobiology, Stubeweg 51, 79108 Freiburg, Germany
    Science 334:686-90. 2011
    b>CENH3 is a centromere-specific histone H3 variant essential for kinetochore assembly. Despite its central role in centromere function, there has been no conclusive evidence supporting CENH3 as sufficient to determine centromere identity...
  78. pmc Assembly of Drosophila centromeric nucleosomes requires CID dimerization
    Weiguo Zhang
    Department of Genome Dynamics, Lawrence Berkeley National Laboratory, University of California, Berkeley, Berkeley, CA 94720, USA
    Mol Cell 45:263-9. 2012
    ..The composition and organization of centromeric nucleosomes containing the essential histone H3 variant CENP-A (CID in Drosophila) is a fundamental, unresolved issue...
  79. pmc Transgenerational propagation and quantitative maintenance of paternal centromeres depends on Cid/Cenp-A presence in Drosophila sperm
    Nitika Raychaudhuri
    Institute of Molecular Life Sciences IMLS, University of Zurich, Zurich, Switzerland
    PLoS Biol 10:e1001434. 2012
    ..In proliferating cells, a centromere-specific histone H3 variant (CenH3), named Cid in Drosophila and Cenp-A in humans, is a crucial component of the epigenetic centromere mark...
  80. pmc Genome-wide analysis reveals a cell cycle-dependent mechanism controlling centromere propagation
    Sylvia Erhardt
    Department of Genome Dynamics, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA
    J Cell Biol 183:805-18. 2008
    ..factors required for centromere propagation using genome-wide RNA interference screening for defects in centromere protein A (CENP-A; centromere identifier [CID]) localization in Drosophila melanogaster...
  81. pmc Concerted action of Aurora B, Polo and NHK-1 kinases in centromere-specific histone 2A phosphorylation
    Amy L Brittle
    The Wellcome Trust Centre for Cell Biology and Institute of Cell Biology, School of Biological Sciences, The University of Edinburgh, Edinburgh, UK
    Exp Cell Res 313:2780-5. 2007
    ..Epistasis analysis indicated that Polo functions upstream of the H2A kinase NHK-1 but parallel to Aurora B. Therefore, multiple mitotic kinases work together to specify the spatial and temporal pattern of H2A T119 phosphorylation...
  82. pmc Single-epitope recognition imaging of native chromatin
    Hongda Wang
    Biodesign Institute, Arizona State University, Tempe, AZ 85287, USA
    Epigenetics Chromatin 1:10. 2008
    ..Recently we applied AFM to native Drosophila chromatin containing the centromere-specific histone 3 (CenH3), showing that it is greatly enriched in smaller particles...
  83. pmc Cis-by-Trans regulatory divergence causes the asymmetric lethal effects of an ancestral hybrid incompatibility gene
    Shamoni Maheshwari
    Department of Molecular Biology and Genetics, Cornell University, Ithaca, New York, United States of America
    PLoS Genet 8:e1002597. 2012
    ..We present a model whereby hybrid lethality occurs by the interaction between evolutionarily ancestral and derived alleles...
  84. pmc Spatiotemporal dynamics of Spc105 regulates the assembly of the Drosophila kinetochore
    Zsolt Venkei
    Department of Genetics, University of Cambridge, Cambridge CB2 3EH, UK
    Open Biol 2:110032. 2012
    ..Our study thus indicates that physical accessibility of kinetochore components plays a crucial role in the regulation of Drosophila kinetochore assembly and leads us to a model in which Spc105 is a licensing factor for its onset...
  85. ncbi Paternal chromosome incorporation into the zygote nucleus is controlled by maternal haploid in Drosophila
    B Loppin
    Centre de Genetique Moleculaire et Cellulaire, CNRS UMR 5534, Universite Claude Bernard Lyon 1, 43, Boulevard du 11 Novembre 1918, Villeurbanne Cedex, 69622, France
    Dev Biol 231:383-96. 2001
    ..The mh phenotype is highly reminiscent of the early developmental defects observed in eggs fertilized by ms(3)K81 mutant males and in eggs produced in incompatible crosses of Drosophila harboring the endosymbiont bacteria Wolbachia...
  86. ncbi Genome-wide variation in the human and fruitfly: a comparison
    C F Aquadro
    Department of Molecular Biology and Genetics, Biotechnology Building, Cornell University, Ithaca, New York 14853, USA
    Curr Opin Genet Dev 11:627-34. 2001
    ..Both species also show lower levels of variation on average in non-African compared to African populations, reflecting a similar evolutionary history and perhaps both natural selection and founder effects in new environments...
  87. ncbi Drosophila melanogaster and D. simulans rescue strains produce fit offspring, despite divergent centromere-specific histone alleles
    A Sainz
    Department of Biological Sciences, University of Notre Dame, Notre Dame, IN 46556, USA
    Heredity (Edinb) 91:28-35. 2003
    ..In Drosophila the centromere-specific histone, Cid (centromere identifier), shows extensive sequence divergence between D. melanogaster and the D...
  88. ncbi RNAi analysis reveals an unexpected role for topoisomerase II in chromosome arm congression to a metaphase plate
    Chih Jui Chang
    Wellcome Trust Centre for Cell Biology, Institute for Cell and Molecular Biology, Kings Buildings, University of Edinburgh, Mayfield Road, Edinburgh EH9 3JR, Scotland, UK
    J Cell Sci 116:4715-26. 2003
    ..This is not kinetochore-based movement, as the centromere of the affected chromosome is located on the plate. This observation raises the possibility that further unexpected functions for Topo II may remain to be discovered...
  89. pmc Australin: a chromosomal passenger protein required specifically for Drosophila melanogaster male meiosis
    Shan Gao
    Department of Zoology, University of Oxford, OX1 3PS Oxford, England, UK
    J Cell Biol 180:521-35. 2008
    ....
  90. pmc Augmin: a protein complex required for centrosome-independent microtubule generation within the spindle
    Gohta Goshima
    Institute for Advanced Research, Nagoya University, Chikusa ku, Nagoya 464 8601, Japan
    J Cell Biol 181:421-9. 2008
    ..Our results suggest that an important mitotic function for gamma-tubulin may lie within the spindle, where augmin and gamma-tubulin function cooperatively to amplify the number of microtubules...
  91. doi Drosophila mars is required for organizing kinetochore microtubules during mitosis
    Ching Po Yang
    Department of Life Science, Tunghai University, Taichung, Taiwan, ROC
    Exp Cell Res 314:3209-20. 2008
    ..In summary, this study has extended our previous study of Mars in cell cycle regulation and provided further evidence showing that Mars is required for the assembly of kinetochore microtubules...
  92. doi Chromator is required for proper microtubule spindle formation and mitosis in Drosophila
    Yun Ding
    Department of Biochemistry, Biophysics, and Molecular Biology, 3156 Molecular Biology Building, Iowa State University, Ames, IA 50011, USA
    Dev Biol 334:253-63. 2009
    ....
  93. pmc DNA replication times the cell cycle and contributes to the mid-blastula transition in Drosophila embryos
    Mark L McCleland
    Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, CA 94143, USA
    J Cell Biol 187:7-14. 2009
    ..We conclude that normal S phase and replication checkpoint activities are important timers of the undisturbed cell cycle before, but not after, the MBT...
  94. pmc ISWI is a RanGTP-dependent MAP required for chromosome segregation
    Hideki Yokoyama
    European Molecular Biology Laboratory, Heidelberg 69117, Germany
    J Cell Biol 187:813-29. 2009
    ..Our results demonstrate that distinctly from its role in spindle assembly, RanGTP maintains spindle MTs in anaphase through the local activation of ISWI and that this is essential for proper chromosome segregation...
  95. ncbi A distal heterochromatic block displays centromeric activity when detached from a natural centromere
    J S Platero
    Howard Hughes Medical Institute, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA
    Mol Cell 4:995-1004. 1999
    ..Our findings suggest that centromere competence is innate to satellite-containing blocks of heterochromatin, challenging models for centromere identity in which competence is an acquired characteristic...
  96. ncbi Domain organization at the centromere and neocentromere
    K H Choo
    The Murdoch Childrens Research Institute, Royal Children s Hospital, Melbourne, Victoria, Australia
    Dev Cell 1:165-77. 2001
    ..Studies in different organisms point to a model of conserved pattern of organization for these domains...
  97. ncbi Speciation and centromere evolution
    P M Borodin
    Science 294:2478-80. 2001
  98. pmc MAST/Orbit has a role in microtubule-kinetochore attachment and is essential for chromosome alignment and maintenance of spindle bipolarity
    Helder Maiato
    Instituto de Ciencias Biomedicas de Abel Salazar, Instituto de Biologia Molecular e Celular, Universidade do Porto, 4150 180, Portugal
    J Cell Biol 157:749-60. 2002
    ..Together, these results strongly support the conclusion that MAST/Orbit is required for microtubules to form functional attachments to kinetochores and to maintain spindle bipolarity...
  99. ncbi Chromatin as a eukaryotic template of genetic information
    Giacomo Cavalli
    Institut de Génétique Humaine CNRS, 141 rue de la Cardonille, 34396 Montpellier Cedex 5, France
    Curr Opin Cell Biol 14:269-78. 2002
    ....
  100. ncbi The dynamic localisation of the Drosophila APC/C: evidence for the existence of multiple complexes that perform distinct functions and are differentially localised
    Jun Yong Huang
    Wellcome Trust Cancer Research UK Institute and Department of Genetics, University of Cambridge, UK
    J Cell Sci 115:2847-56. 2002
    ..Taken together, our results raise the intriguing possibility that there could be multiple forms of the APC/C that are differentially localised and perform distinct functions...
  101. pmc DNA hypermethylation in Drosophila melanogaster causes irregular chromosome condensation and dysregulation of epigenetic histone modifications
    Frank Weissmann
    Research Group Epigenetics, Deutsches Krebsforschungszentrum, 69120 Heidelberg, Germany
    Mol Cell Biol 23:2577-86. 2003
    ..Our results thus uncover the molecular consequences of genomic hypermethylation and demonstrate a mutual interaction between DNA methylation and histone methylation...

Research Grants7

  1. Epigenetic Regulation of Gene Expression During Early Mouse Embryogenesis
    M Smith; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  2. Mechanism of Meiotic Pairing in Drosophila
    BRUCE MCKEE; Fiscal Year: 2007
    ..3) What is the molecular basis for homologous pairing in early prophase. 4) How are homologous pairing and conjunction related? We will use predominant genetic and cell biological methods to address these questions. ..
  3. The Role of MYST histone acetyltransferases in genome stability
    M Smith; Fiscal Year: 2008
    ..These experiments will greatly expand our understanding of Hbo1 function in regulating DNA replication and cell proliferation. ..
  4. ALIGNMENT BASED METHODS FOR MOLECULAR SEQUENCE ANALYSIS
    Steven Henikoff; Fiscal Year: 2001
    ..This effort could yield new eukaryotic family members of great importance for understanding epigenetic phenomena, some of which, such as parental imprinting, are relevant to human disease. ..
  5. Maintenance of blocks-based tools for functional genomic
    Steven Henikoff; Fiscal Year: 2006
    ..These various components will be packaged to facilitate updates and local implementations. [unreadable] [unreadable]..
  6. HISTONE GENE EXPRESSION IN YEAST
    M Smith; Fiscal Year: 2007
    ..To understand the rules and mechanisms for these assembly patterns, we will engineer mutations in specific cis- and trans-acting factors to test alternative models of Htz1 recruitment. ..
  7. COMPARATIVE MOLECULAR POPULATION GENETICS OF DROSOPHILA
    Charles F Aquadro; Fiscal Year: 2010
    ..Our results lay the foundation for understanding the frequency and molecular basis of adaptation in Drosophila, providing a benchmark against which to test our assumptions about adaptive evolution in other genomes. ..