yidC

Summary

Gene Symbol: yidC
Description: membrane protein insertase
Alias: ECK3698, JW3683
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. ncbi Phylogenetic and structural analyses of the oxa1 family of protein translocases
    M R Yen
    Department of Biology, University of California at San Diego, La Jolla, CA 92093 0116, USA
    FEMS Microbiol Lett 204:223-31. 2001
  2. ncbi Reconstitution of phospholipid flippase activity from E. coli inner membrane: a test of the protein translocon as a candidate flippase
    William E Watkins
    Department of Biochemistry, University of Wisconsin Madison, 53706 1569, USA
    Biol Chem 383:1435-40. 2002
  3. ncbi YidC, a newly defined evolutionarily conserved protein, mediates membrane protein assembly in bacteria
    Minyong Chen
    Department of Chemistry, The Ohio State University, Columbus, OH 43210 1185, USA
    Biol Chem 383:1565-72. 2002
  4. ncbi Versatility of inner membrane protein biogenesis in Escherichia coli
    Linda Fröderberg
    Department of Biochemistry and Biophysics, Arrhenius Laboratories, Stockholm University, SE 106 91 Stockholm, Sweden
    Mol Microbiol 47:1015-27. 2003
  5. pmc Secretion defects that activate the phage shock response of Escherichia coli
    Susan E Jones
    Department of Biological Sciences, Imperial College London, South Kensington Campus, London SW7 2AZ, United Kingdom
    J Bacteriol 185:6707-11. 2003
  6. ncbi The Sec-independent function of Escherichia coli YidC is evolutionary-conserved and essential
    Edwin van Bloois
    Department of Molecular Microbiology, Institute of Molecular Cell Biology, Vrije Universiteit, De Boelelaan 1087, 1081 HV Amsterdam, The Netherlands
    J Biol Chem 280:12996-3003. 2005
  7. ncbi Direct interaction of YidC with the Sec-independent Pf3 coat protein during its membrane protein insertion
    Minyong Chen
    Department of Chemistry, Ohio State Biochemistry Program, and Protein Research Group, The Ohio State University, Columbus, Ohio 43210, USA
    J Biol Chem 277:7670-5. 2002
  8. ncbi Chloroplast YidC homolog Albino3 can functionally complement the bacterial YidC depletion strain and promote membrane insertion of both bacterial and chloroplast thylakoid proteins
    Fenglei Jiang
    Department of Chemistry, Molecular Cellular Developmental Biology Program and Protein Research Group, The Ohio State University, Columbus, Ohio 43210, USA
    J Biol Chem 277:19281-8. 2002
  9. ncbi SecDFyajC forms a heterotetrameric complex with YidC
    Nico Nouwen
    Department of Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, 9751 NN Haren, The Netherlands
    Mol Microbiol 44:1397-405. 2002
  10. ncbi YidC and SecY mediate membrane insertion of a Type I transmembrane domain
    Edith N G Houben
    Department of Microbiology, Institute of Molecular Biological Sciences, Vrije Universiteit, De Boelelaan 1087, 1081 HV Amsterdam, The Netherlands
    J Biol Chem 277:35880-6. 2002

Research Grants

Scientific Experts

  • Joen Luirink
  • Nico Nouwen
  • Jan Willem de Gier
  • Andreas Kuhn
  • Rosemary Stuart
  • Hans-Georg Koch
  • P N Danese
  • R E Dalbey
  • Arnold J M Driessen
  • Minyong Chen
  • Liang Yi
  • Martin van der Laan
  • Stefan Kol
  • Edwin van Bloois
  • Edith N G Houben
  • Lu Zhu
  • Fenglei Jiang
  • Zhong Yu
  • Claire E Price
  • Sandra J Facey
  • Samuel Wagner
  • Bauke Oudega
  • Shushi Nagamori
  • Kun Xie
  • Marcia B Goldberg
  • Linda Fröderberg
  • Uwe Gerken
  • Malene L Urbanus
  • J C Samuelson
  • Gregory Koningstein
  • Anabela de Sousa Borges
  • Zaoping Li
  • M van der Laan
  • H Ronald Kaback
  • Nil Celebi
  • Dirk Jan Scheffers
  • Dana Boyd
  • M L Urbanus
  • B Oudega
  • Corinne M ten Hagen-Jongman
  • J Brunner
  • Andrew N Gray
  • P A Scotti
  • Jeanine de Keyzer
  • Jijun Yuan
  • Peng Wang
  • Josef Brunner
  • Ranjana Pathania
  • Sophie Winterfeld
  • Rebecca Kohler
  • Gerda Bär
  • Ian Collinson
  • Yuxia Dong
  • Stephanie Ravaud
  • Daniel Martinez Molina
  • Mirko Lotz
  • David C Oliver
  • Louise Baars
  • Eli O van der Sluis
  • David J F du Plessis
  • E N Houben
  • Nathalie Pradel
  • Justyna Serek
  • A Saaf
  • Lauren D Brandon
  • Susan E Jones
  • Kostas Hatzixanthis
  • Amanda Raine
  • James C Samuelson
  • Annemieke van Dalen
  • William E Watkins
  • Martin Reindl
  • Stephen H White
  • Abdul Wasey
  • Christian Klenner
  • M R Yen
  • K Beck
  • Daniela De Biase
  • A J Driessen
  • M Chen
  • Hironori Niki
  • Gregory M Cook
  • Shirin Sharafi
  • Peter van Ulsen
  • Angela Tramonti
  • F Jiang
  • Joost Teixeira De Mattos
  • Josephine M Henderson-Frost
  • Shirin Shirafi
  • Martijn Bekker

Detail Information

Publications85

  1. ncbi Phylogenetic and structural analyses of the oxa1 family of protein translocases
    M R Yen
    Department of Biology, University of California at San Diego, La Jolla, CA 92093 0116, USA
    FEMS Microbiol Lett 204:223-31. 2001
    ..Multiple sequence alignments reveal conserved residues and provide the basis for structural and phylogenetic analyses that serve to characterize the Oxa1 family...
  2. ncbi Reconstitution of phospholipid flippase activity from E. coli inner membrane: a test of the protein translocon as a candidate flippase
    William E Watkins
    Department of Biochemistry, University of Wisconsin Madison, 53706 1569, USA
    Biol Chem 383:1435-40. 2002
    ..To test this possibility, we used E. coli cells depleted of SecYE or YidC to assay flipping in proteoliposomes reconstituted from detergent extracts of their inner membranes...
  3. ncbi YidC, a newly defined evolutionarily conserved protein, mediates membrane protein assembly in bacteria
    Minyong Chen
    Department of Chemistry, The Ohio State University, Columbus, OH 43210 1185, USA
    Biol Chem 383:1565-72. 2002
    ..Recently, a new membrane component called YidC was discovered that mediates the insertion of proteins into membranes in bacteria...
  4. ncbi Versatility of inner membrane protein biogenesis in Escherichia coli
    Linda Fröderberg
    Department of Biochemistry and Biophysics, Arrhenius Laboratories, Stockholm University, SE 106 91 Stockholm, Sweden
    Mol Microbiol 47:1015-27. 2003
    ..covering most IMP structures possible has been studied in a variety of signal recognition particle (SRP), Sec and YidC mutant strains. We show that the assembly of the complete set of model IMPs is assisted (i.e...
  5. pmc Secretion defects that activate the phage shock response of Escherichia coli
    Susan E Jones
    Department of Biological Sciences, Imperial College London, South Kensington Campus, London SW7 2AZ, United Kingdom
    J Bacteriol 185:6707-11. 2003
    ..Here we show that defects in yidC and sec secretion induce psp but that defects in tat and srp have no effect...
  6. ncbi The Sec-independent function of Escherichia coli YidC is evolutionary-conserved and essential
    Edwin van Bloois
    Department of Molecular Microbiology, Institute of Molecular Cell Biology, Vrije Universiteit, De Boelelaan 1087, 1081 HV Amsterdam, The Netherlands
    J Biol Chem 280:12996-3003. 2005
    b>YidC plays a role in the integration and assembly of many (if not all) Escherichia coli inner membrane proteins...
  7. ncbi Direct interaction of YidC with the Sec-independent Pf3 coat protein during its membrane protein insertion
    Minyong Chen
    Department of Chemistry, Ohio State Biochemistry Program, and Protein Research Group, The Ohio State University, Columbus, Ohio 43210, USA
    J Biol Chem 277:7670-5. 2002
    b>YidC is a newly defined translocase component that mediates the insertion of proteins into the membrane bilayer. How YidC functions in the insertion process is not known...
  8. ncbi Chloroplast YidC homolog Albino3 can functionally complement the bacterial YidC depletion strain and promote membrane insertion of both bacterial and chloroplast thylakoid proteins
    Fenglei Jiang
    Department of Chemistry, Molecular Cellular Developmental Biology Program and Protein Research Group, The Ohio State University, Columbus, Ohio 43210, USA
    J Biol Chem 277:19281-8. 2002
    A new component of the bacterial translocation machinery, YidC, has been identified that specializes in the integration of membrane proteins...
  9. ncbi SecDFyajC forms a heterotetrameric complex with YidC
    Nico Nouwen
    Department of Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, 9751 NN Haren, The Netherlands
    Mol Microbiol 44:1397-405. 2002
    ..SecD, SecF, and YajC form another heterotrimeric complex that can associate with the SecYEG complex. YidC is an essential membrane protein that plays a role in the integration of newly synthesized membrane proteins, and ..
  10. ncbi YidC and SecY mediate membrane insertion of a Type I transmembrane domain
    Edith N G Houben
    Department of Microbiology, Institute of Molecular Biological Sciences, Vrije Universiteit, De Boelelaan 1087, 1081 HV Amsterdam, The Netherlands
    J Biol Chem 277:35880-6. 2002
    b>YidC has been identified recently as an evolutionary conserved factor that is involved in the integration of inner membrane proteins (IMPs) in Escherichia coli...
  11. ncbi Insertion of proteins into the inner membrane of mitochondria: the role of the Oxa1 complex
    Rosemary Stuart
    Department of Biology, Marquette University, 530 N 15th Street, Milwaukee, WI 53233, USA
    Biochim Biophys Acta 1592:79-87. 2002
    ..Oxa1 is a member of the conserved Oxa1/YidC/Alb3 protein family found throughout prokaryotes throughout eukaryotes (where it is found in mitochondria and ..
  12. ncbi Oxa1/Alb3/YidC system for insertion of membrane proteins in mitochondria, chloroplasts and bacteria (review)
    Liang Yi
    Department of Chemistry, The Ohio State University, Columbus, Ohio 43210, USA
    Mol Membr Biol 22:101-11. 2005
    ..In this pathway, the Oxa1/Alb3/YidC proteins are believed to function as membrane insertases that play an important role in the membrane protein ..
  13. ncbi The sensor protein KdpD inserts into the Escherichia coli membrane independent of the Sec translocase and YidC
    Sandra J Facey
    Institute of Microbiology and Molecular Biology, University of Hohenheim, Stuttgart, Germany
    Eur J Biochem 270:1724-34. 2003
    ..The results show that neither the Sec translocase nor the YidC protein are required for membrane insertion of the second loop of KdpD...
  14. ncbi Targeting, insertion, and localization of Escherichia coli YidC
    Malene L Urbanus
    Department of Microbiology, Institute for Molecular Biological Sciences, De Boelelaan 1087, 1081 HV Amsterdam, The Netherlands
    J Biol Chem 277:12718-23. 2002
    b>YidC was recently shown to play an important role in the assembly of inner membrane proteins (IMPs) both in conjunction with and separate from the Sec-translocon...
  15. pmc A conserved function of YidC in the biogenesis of respiratory chain complexes
    M van der Laan
    Department of Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Kerklaan 30, 9751 NN Haren, The Netherlands
    Proc Natl Acad Sci U S A 100:5801-6. 2003
    The Escherichia coli inner membrane protein (IMP) YidC is involved in the membrane integration of IMPs both in concert with and independently from the Sec translocase...
  16. ncbi YidC is strictly required for membrane insertion of subunits a and c of the F(1)F(0)ATP synthase and SecE of the SecYEG translocase
    Liang Yi
    Department of Chemistry, The Ohio State University, Columbus, Ohio 43210, USA
    Biochemistry 42:10537-44. 2003
    b>YidC was previously discovered to play a critical role for the insertion of the Sec-independent M13 procoat and Pf3 coat phage proteins into the Escherichia coli inner membrane...
  17. pmc F1F0 ATP synthase subunit c is a substrate of the novel YidC pathway for membrane protein biogenesis
    Martin van der Laan
    Dept of Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Kerklaan 30, 9751 NN Haren, Netherlands
    J Cell Biol 165:213-22. 2004
    The Escherichia coli YidC protein belongs to the Oxa1 family of membrane proteins that have been suggested to facilitate the insertion and assembly of membrane proteins either in cooperation with the Sec translocase or as a separate ..
  18. ncbi The Alb3/Oxa1/YidC protein family: membrane-localized chaperones facilitating membrane protein insertion?
    Andreas Kuhn
    Institute of Microbiology and Molecular Biology, University of Hohenheim, 70599 Stuttgart, Germany
    Trends Cell Biol 13:510-6. 2003
    The recently identified Alb3/Oxa1/YidC family constitutes a novel class of proteins that function in promoting membrane insertion in chloroplasts, mitochondria and bacteria...
  19. ncbi SecYEG proteoliposomes catalyze the Deltaphi-dependent membrane insertion of FtsQ
    Martin van der Laan
    Department of Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Kerklaan 30, 9751 NN Haren, The Netherlands
    J Biol Chem 279:1659-64. 2004
    ..potential are essential for the translocation of the large periplasmic domain of FtsQ, whereas co-reconstituted YidC has an inhibitory effect...
  20. pmc Role of YidC in folding of polytopic membrane proteins
    Shushi Nagamori
    5 748 MacDonald Research Laboratories, Rm 6720, P O Box 951662, Howard Hughes Medical Institute, University of California, Los Angeles, Los Angeles, CA 90095 1662, USA
    J Cell Biol 165:53-62. 2004
    b>YidC of Echerichia coli, a member of the conserved Alb3/Oxa1/YidC family, is postulated to be important for biogenesis of membrane proteins...
  21. pmc Escherichia coli YidC is a membrane insertase for Sec-independent proteins
    Justyna Serek
    Institute of Microbiology and Molecular Biology, University of Hohenheim, Stuttgart, Germany
    EMBO J 23:294-301. 2004
    b>YidC is a recently discovered bacterial membrane protein that is related to the mitochondrial Oxa1p and the Alb3 protein of chloroplasts...
  22. ncbi Function of YidC for the insertion of M13 procoat protein in Escherichia coli: translocation of mutants that show differences in their membrane potential dependence and Sec requirement
    J C Samuelson
    Department of Chemistry, Ohio State University, Columbus, Ohio 43210, USA
    J Biol Chem 276:34847-52. 2001
    ..M13 Procoat protein in bacteria requires the membrane electrochemical potential and the integral membrane protein YidC. We show here that YidC is involved in the translocation but not in the targeting of the Procoat protein, because ..
  23. ncbi YidC/Oxa1p/Alb3: evolutionarily conserved mediators of membrane protein assembly
    J Luirink
    Department of Microbiology, BioCentrum Amsterdam, The Netherlands
    FEBS Lett 501:1-5. 2001
    ..This factor is designated YidC in Escherichia coli, and is localized in the inner membrane...
  24. doi Mechanisms of YidC-mediated insertion and assembly of multimeric membrane protein complexes
    Stefan Kol
    Department of Molecular Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, the Zernike Institute of Advanced Materials, The Netherlands
    J Biol Chem 283:31269-73. 2008
    The YidC protein fulfills a dual and essential role in the assembly of inner membrane proteins in Escherichia coli...
  25. ncbi Membrane topology of the 60-kDa Oxa1p homologue from Escherichia coli
    A Saaf
    Department of Biochemistry, Stockholm University, S 106 91 Stockholm, Sweden
    J Biol Chem 273:30415-8. 1998
    ..In contrast to partial N-terminal fusion protein constructs, the full-length protein folds into a protease-resistant conformation, suggesting that important folding determinants are present in the C-terminal part of the molecule...
  26. ncbi Biogenesis of inner membrane proteins in Escherichia coli
    J W de Gier
    Department of Biochemistry, Stockholm University, 106 91 Stockholm, Sweden
    Mol Microbiol 40:314-22. 2001
    ..Here, we review and discuss recent advances in our understanding of the biogenesis of inner membrane proteins in E. coli...
  27. ncbi YidC mediates membrane protein insertion in bacteria
    J C Samuelson
    Department of Chemistry, The Ohio State University, Columbus 43210, USA
    Nature 406:637-41. 2000
    ..Here we show that membrane insertion of two Sec-independent proteins requires YidC. YidC is essential for E. coli viability and homologues are present in mitochondria and chloroplasts...
  28. pmc YidC, the Escherichia coli homologue of mitochondrial Oxa1p, is a component of the Sec translocase
    P A Scotti
    Department of Microbiology, Institute of Molecular Biological Sciences, BioCentrum Amsterdam, De Boelelaan 1087, 1081 HV Amsterdam, The Netherlands
    EMBO J 19:542-9. 2000
    ..signal anchor sequence contacts both lipids and a novel 60 kDa translocase-associated component that we identify as YidC. YidC is homologous to Saccharomyces cerevisiae Oxa1p, which has been shown to function in a novel export pathway ..
  29. pmc YidC, an assembly site for polytopic Escherichia coli membrane proteins located in immediate proximity to the SecYE translocon and lipids
    K Beck
    Institut fur Biochemie und Molekularbiologie, Universitat Freiburg, Hermann Herderstrasse 7, D 79104 Freiburg, Germany
    EMBO Rep 2:709-14. 2001
    Like its mitochondrial homolog Oxa1p, the inner membrane protein YidC of Escherichia coli is involved in the integration of membrane proteins. We have analyzed individual insertion steps of the polytopic E...
  30. doi YidC is involved in the biogenesis of anaerobic respiratory complexes in the inner membrane of Escherichia coli
    Claire E Price
    Department of Molecular Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Kerklaan 30, NL 9751 NN Haren, The Netherlands
    J Biol Chem 283:26921-7. 2008
    b>YidC of Escherichia coli belongs to the evolutionarily conserved Oxa1/Alb3/YidC family. Members of this family have all been implicated in membrane protein biogenesis of aerobic respiratory and energy-transducing proteins...
  31. ncbi Sec/SRP requirements and energetics of membrane insertion of subunits a, b, and c of the Escherichia coli F1F0 ATP synthase
    Liang Yi
    Department of Chemistry, The Ohio State University, Columbus, Ohio 43210, USA
    J Biol Chem 279:39260-7. 2004
    Previously, the role of YidC in the membrane protein biogenesis of the F(0) sector of the Escherichia coli F(1)F(0) ATP synthase was investigated...
  32. doi Biogenesis of MalF and the MalFGK(2) maltose transport complex in Escherichia coli requires YidC
    Samuel Wagner
    Center for Biomembrane Research, Department of Biochemistry and Biophysics, Stockholm University, Stockholm, Sweden
    J Biol Chem 283:17881-90. 2008
    ..MalF is targeted via the SRP pathway to the Sec/YidC insertion site...
  33. ncbi The mechanosensitive channel protein MscL is targeted by the SRP to the novel YidC membrane insertion pathway of Escherichia coli
    Sandra J Facey
    Institute of Microbiology, University of Hohenheim, 70599 Stuttgart, Germany
    J Mol Biol 365:995-1004. 2007
    ..However, depletion of the membrane insertase YidC inhibits translocation of the protein across the membrane...
  34. pmc Reconstitution of Sec-dependent membrane protein insertion: nascent FtsQ interacts with YidC in a SecYEG-dependent manner
    M van der Laan
    Department of Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Kerklaan 30, 9751 NN Haren The Netherlands
    EMBO Rep 2:519-23. 2001
    The inner membrane protein YidC is associated with the preprotein translocase of Escherichia coli and contacts transmembrane segments of nascent inner membrane proteins during membrane insertion...
  35. pmc Sec-dependent membrane protein insertion: sequential interaction of nascent FtsQ with SecY and YidC
    M L Urbanus
    Department of Microbiology, Institute of Molecular Biological Sciences, De Boelelaan 1087, 1081 HV Amsterdam, The Netherlands
    EMBO Rep 2:524-9. 2001
    Recent studies identified YidC as a novel membrane factor that may play a key role in membrane insertion of inner membrane proteins (IMPs), both in conjunction with the Sec-translocase and as a separate entity...
  36. pmc Conserved negative charges in the transmembrane segments of subunit K of the NADH:ubiquinone oxidoreductase determine its dependence on YidC for membrane insertion
    Claire E Price
    Department of Molecular Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, the Zernike Institute for Advanced Materials, University of Groningen, Kerklaan 30, NL 9751 NN Haren, The Netherlands
    J Biol Chem 285:3575-81. 2010
    All members of the Oxa1/Alb3/YidC family have been implicated in the biogenesis of respiratory and energy transducing proteins. In Escherichia coli, YidC functions together with and independently of the Sec system...
  37. ncbi Projection structure of yidC: a conserved mediator of membrane protein assembly
    Mirko Lotz
    Max Planck Institute of Biophysics, Max von Laue Strasse 3, D 60438 Frankfurt am Main, Germany
    J Mol Biol 375:901-7. 2008
    ..In Escherichia coli, yidC is required for membrane insertion, acting in both a Sec-dependent and a Sec-independent manner...
  38. pmc The ribosome and YidC. New insights into the biogenesis of Escherichia coli inner membrane proteins
    Jan Willem L de Gier
    Department of Biochemistry and Biophysics, Arrhenius Laboratories, Stockholm University, SE 106 91 Stockholm, Sweden
    EMBO Rep 4:939-43. 2003
    ..Lateral transport seems to be assisted by the IMP YidC. In this article, we discuss recent observations that point to a key role for the ribosome in IMP targeting and to ..
  39. ncbi Nascent Lep inserts into the Escherichia coli inner membrane in the vicinity of YidC, SecY and SecA
    E N Houben
    Department of Microbiology, Institute of Molecular Biological Sciences, BioCentrum Amsterdam, The Netherlands
    FEBS Lett 476:229-33. 2000
    ..Lep 100-mer was in the vicinity of SecA and SecY, whereas the first transmembrane domain H1 was in the vicinity of YidC. These results suggested that YidC, together with the Sec translocase, functions in the assembly of Lep...
  40. ncbi Defining the regions of Escherichia coli YidC that contribute to activity
    Fenglei Jiang
    Department of Chemistry, Ohio State University, Columbus, Ohio 43210, USA
    J Biol Chem 278:48965-72. 2003
    The YidC/Oxa1/Alb3 family of proteins catalyzes membrane protein insertion in bacteria, mitochondria, and chloroplasts...
  41. ncbi Targeting and insertion of heterologous membrane proteins in E. coli
    Amanda Raine
    Department of Pharmaceutical Biosciences, Uppsala University, BMC, Box 591, 75124, Uppsala, Sweden
    Biochimie 85:659-68. 2003
    ..Furthermore, we demonstrate that nascent chains of Bop and MC(4)R first interact with SecY and then with YidC as they move through the translocon...
  42. pmc The Escherichia coli membrane protein insertase YidC assists in the biogenesis of penicillin binding proteins
    Anabela de Sousa Borges
    Molecular Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute and Zernike Institute for Advanced Materials, University of Groningen, Groningen, The Netherlands
    J Bacteriol 197:1444-50. 2015
    ..The Sec translocon and the YidC insertase are responsible for the insertion of the majority of proteins into the cytoplasmic membrane...
  43. pmc Activators of the glutamate-dependent acid resistance system alleviate deleterious effects of YidC depletion in Escherichia coli
    Zhong Yu
    Department of Molecular Microbiology, Institute of Molecular Cell Biology, VU University Amsterdam, Amsterdam 1081 HV, The Netherlands
    J Bacteriol 193:1308-16. 2011
    The function of the essential inner membrane protein (IMP) YidC in Escherichia coli has been studied for a limited number of model IMPs and primarily using targeted approaches...
  44. pmc Identification of YidC residues that define interactions with the Sec Apparatus
    Zaoping Li
    Division of Infectious Diseases, Department of Medicine, Massachusetts General Hospital, Cambridge, Massachusetts, USA
    J Bacteriol 196:367-77. 2014
    ..the membrane via the Sec apparatus with the assistance of the widely conserved essential membrane protein insertase YidC. After threading into the SecYEG translocon, transmembrane segments of nascent proteins are thought to exit the ..
  45. pmc Charge composition features of model single-span membrane proteins that determine selection of YidC and SecYEG translocase pathways in Escherichia coli
    Lu Zhu
    Department of Chemistry and Biochemistry, Ohio State University, Columbus, Ohio 43210, USA
    J Biol Chem 288:7704-16. 2013
    ..of single-span model membrane proteins based upon leader peptidase that determines whether the proteins insert by a YidC/Sec-independent, YidC-only, or YidC/Sec mechanism...
  46. doi Both YidC and SecYEG are required for translocation of the periplasmic loops 1 and 2 of the multispanning membrane protein TatC
    Lu Zhu
    Department of Chemistry and Biochemistry, The Ohio State University, Columbus, OH 43210, USA
    J Mol Biol 424:354-67. 2012
    ..While translocation of loops 1 and 2 is strictly dependent on the Sec translocase and the YidC insertase, translocation of loop 3 does not depend on the translocase or insertase...
  47. pmc Unbalanced charge distribution as a determinant for dependence of a subset of Escherichia coli membrane proteins on the membrane insertase YidC
    Andrew N Gray
    Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts, USA
    MBio 2:. 2011
    ..To function properly, they must be inserted into the membrane and folded correctly. YidC, an essential protein in Escherichia coli with homologues in other bacteria, Archaea, mitochondria, and ..
  48. doi Chemical genomics in Escherichia coli identifies an inhibitor of bacterial lipoprotein targeting
    Ranjana Pathania
    Department of Biochemistry and Biomedical Sciences, McMaster University, Hamilton, Ontario, Canada
    Nat Chem Biol 5:849-56. 2009
    ..We show that MAC13243 inhibits the function of the LolA protein and represents a new chemical probe of lipoprotein targeting in bacteria with promise as an antibacterial lead with Gram-negative selectivity...
  49. doi The crystal structure of the periplasmic domain of the Escherichia coli membrane protein insertase YidC contains a substrate binding cleft
    Stephanie Ravaud
    Biochemie Zentrum der Universitat Heidelberg, Im Neuenheimer Feld 328, Heidelberg, Germany
    J Biol Chem 283:9350-8. 2008
    ..b>YidC is an essential membrane protein involved in the insertion of inner membrane proteins together with the Sec ..
  50. doi Detection of cross-links between FtsH, YidC, HflK/C suggests a linked role for these proteins in quality control upon insertion of bacterial inner membrane proteins
    Edwin van Bloois
    Department of Molecular Microbiology, Institute of Molecular Cell Biology, Vrije Universiteit, De Boelelaan 1087, 1081 HV Amsterdam, The Netherlands
    FEBS Lett 582:1419-24. 2008
    ..Here, we demonstrate that YidC and FtsH are adjacent to a nascent, truncated membrane protein using in vitro photo cross-linking...
  51. ncbi Targeting and assembly of periplasmic and outer-membrane proteins in Escherichia coli
    P N Danese
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138, USA
    Annu Rev Genet 32:59-94. 1998
    ..We also discuss the various signal transduction systems that monitor extracytoplasmic protein folding and targeting, and we consider how these signal transduction systems may ultimately control these processes...
  52. doi Initial binding process of the membrane insertase YidC with its substrate Pf3 coat protein is reversible
    Uwe Gerken
    Institute of Microbiology, University of Hohenheim, Garbenstrasse 30, D 70599 Stuttgart, Germany
    Biochemistry 47:6052-8. 2008
    The binding of the inner membrane insertase YidC from Escherichia coli to its substrate, the Pf3 coat protein, was examined in vitro by fluorescence spectroscopy...
  53. doi Expression and purification of the recombinant membrane protein YidC: a case study for increased stability and solubility
    Daniel Martinez Molina
    Division of Biophysics, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden
    Protein Expr Purif 62:49-52. 2008
    b>YidC is an inner membrane protein from Escherichia coli and is an essential component in insertion, translocation and assembly of membrane proteins in the membranes...
  54. pmc The SecDFyajC domain of preprotein translocase controls preprotein movement by regulating SecA membrane cycling
    F Duong
    Dartmouth Medical School, Department of Biochemistry, Hanover, NH 03755, USA
    EMBO J 16:4871-9. 1997
    ..These findings establish a functional relationship between SecA membrane insertion and preprotein translocation and show that SecDFyajC controls SecA membrane cycling to regulate the movement of the translocating preprotein...
  55. pmc SecD and SecF facilitate protein export in Escherichia coli
    J A Pogliano
    Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, MA 02115
    EMBO J 13:554-61. 1994
    ..Our results provide additional support for the notion that an early step in protein export is cold-sensitive...
  56. pmc The conserved third transmembrane segment of YidC contacts nascent Escherichia coli inner membrane proteins
    Zhong Yu
    Department of Molecular Microbiology, Institute of Molecular Cell Biology, VU University, De Boelelaan 1085, 1081 HV Amsterdam, The Netherlands
    J Biol Chem 283:34635-42. 2008
    Escherichia coli YidC is a polytopic inner membrane protein that plays an essential and versatile role in the biogenesis of inner membrane proteins...
  57. doi Substrate-induced conformational change of the Escherichia coli membrane insertase YidC
    Sophie Winterfeld
    Institute of Microbiology, University of Hohenheim, Garbenstrasse 30, D 70599 Stuttgart, Germany
    Biochemistry 48:6684-91. 2009
    The membrane insertase YidC from Escherichia coli reversibly binds its substrate Pf3 coat protein...
  58. pmc Functional overlap but lack of complete cross-complementation of Streptococcus mutans and Escherichia coli YidC orthologs
    Yuxia Dong
    Department of Chemistry, The Ohio State University, Columbus, Ohio 43210, USA
    J Bacteriol 190:2458-69. 2008
    Oxa/YidC/Alb family proteins are chaperones involved in membrane protein insertion and assembly. Streptococcus mutans has two YidC paralogs...
  59. pmc SecF stabilizes SecD and SecY, components of the protein translocation machinery of the Escherichia coli cytoplasmic membrane
    K Sagara
    Institute of Molecular and Cellular Biosciences, University of Tokyo, Japan
    J Bacteriol 176:4111-6. 1994
    ..It is suggested that SecF interacts with both SecD and SecY. SecE-SecY interaction has been demonstrated. It is likely, therefore, that all Sec proteins in the cytoplasmic membrane interact with each other...
  60. pmc Global change of gene expression and cell physiology in YidC-depleted Escherichia coli
    Peng Wang
    The Ohio State University, Department of Chemistry, 100 West 18th Avenue, Columbus, OH 43210, USA
    J Bacteriol 192:2193-209. 2010
    b>YidC depletion affects membrane protein insertion and leads to a defect in the growth of the Escherichia coli cell...
  61. pmc Genetic and molecular characterization of the Escherichia coli secD operon and its products
    K J Pogliano
    Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts 02115
    J Bacteriol 176:804-14. 1994
    ..TnphoA fusions to SecD and SecF have also been recombined onto the chromosome and used to determine the level of these proteins within the cell. Our results indicate that there are fewer than 30 SecD and SecF molecules per cell...
  62. pmc secD, a new gene involved in protein export in Escherichia coli
    C Gardel
    J Bacteriol 169:1286-90. 1987
    ..A plasmid carrying secD+ was identified and used to show that the mutations are recessive. The secD gene may code for a component of the cellular export machinery...
  63. ncbi Physical mapping and nucleotide sequence of the rnpA gene that encodes the protein component of ribonuclease P in Escherichia coli
    F G Hansen
    Gene 38:85-93. 1985
    ..Comparison with previously published data on the transcription of rpmH suggests that the rnpA gene is the second gene in the rpmH operon...
  64. pmc Early encounters of a nascent membrane protein: specificity and timing of contacts inside and outside the ribosome
    Edith N G Houben
    Department of Molecular Microbiology, Institute of Molecular Cell Biology, Vrije Universiteit, 1081 HV Amsterdam, Netherlands
    J Cell Biol 170:27-35. 2005
    ..particle (SRP) started to interact with the nascent IMP and to target the ribosome-nascent chain complex to the Sec-YidC complex in the inner membrane when maximally half of the transmembrane domain (TM) was exposed from the ribosomal ..
  65. ncbi A subset of bacterial inner membrane proteins integrated by the twin-arginine translocase
    Kostas Hatzixanthis
    Centre for Metalloprotein Spectroscopy and Biology, School of Biological Sciences, University of East Anglia, Norwich, NR4 7TJ, UK
    Mol Microbiol 49:1377-90. 2003
    ..membrane proteins are assembled by a co-translational process directed by SRP/FtsY, the SecYEG translocase, and YidC. In this work we define a novel class of integral membrane proteins assembled by a Tat-dependent mechanism...
  66. ncbi Features of transmembrane segments that promote the lateral release from the translocase into the lipid phase
    Kun Xie
    Department of Chemistry, The Ohio State University, 100 W 18th Avenue, Columbus, Ohio 43210, USA
    Biochemistry 46:15153-61. 2007
    ..reverse signal-anchor, and stop-transfer sequences in bacterial membrane proteins that use either the YidC or SecYEG translocases for membrane insertion...
  67. ncbi YidC-dependent translocation of green fluorescence protein fused to the FliP cleavable signal peptide
    Nathalie Pradel
    Laboratoire de Chimie Bacterienne, UPR9043, IBSM, CNRS, 31, Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
    Biochimie 87:191-6. 2005
    ..In addition, we showed for the first time the involvement of YidC in protein translocation across the inner membrane.
  68. ncbi Flexibility in targeting and insertion during bacterial membrane protein biogenesis
    Edwin van Bloois
    Department of Molecular Microbiology, Institute of Molecular Cell Biology, Vrije Universiteit, De Boelelaan 1085, 1081 HV Amsterdam, The Netherlands
    Biochem Biophys Res Commun 362:727-33. 2007
    ..assisted by targeting and insertion factors such as the signal recognition particle (SRP), the Sec-translocon and YidC with translocation of (large) periplasmic domains energized by SecA and the proton motive force (pmf)...
  69. ncbi Consequences of membrane protein overexpression in Escherichia coli
    Samuel Wagner
    Department of Biochemistry and Biophysics, Center for Biomembrane Research, Stockholm University, SE 106 91, Stockholm, Sweden
    Mol Cell Proteomics 6:1527-50. 2007
    ..Therefore, we analyzed the consequences of overexpression of three different membrane proteins (YidC, YedZ, and LepI) fused to green fluorescent protein (GFP) in the bacterium Escherichia coli and compared this with ..
  70. ncbi Subunit a of cytochrome o oxidase requires both YidC and SecYEG for membrane insertion
    David J F du Plessis
    Department of Molecular Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute and the Materials Science Center Plus, University of Groningen, 9751 NN Haren, The Netherlands
    J Biol Chem 281:12248-52. 2006
    The Escherichia coli YidC protein belongs to the Oxa1 family of membrane proteins that facilitate the insertion of membrane proteins. Depletion of YidC in E...
  71. ncbi Stepwise evolution of the Sec machinery in Proteobacteria
    Eli O van der Sluis
    Department of Molecular Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, 9751 NN Haren, The Netherlands
    Trends Microbiol 14:105-8. 2006
    ..The Sec machinery of Escherichia coli and other Enterobacteriales represents the end product of this stepwise evolution...
  72. ncbi Membrane biogenesis of subunit II of cytochrome bo oxidase: contrasting requirements for insertion of N-terminal and C-terminal domains
    Nil Celebi
    Department of Chemistry, The Ohio State University, Columbus, OH 43210, USA
    J Mol Biol 357:1428-36. 2006
    The membrane assembly of the respiratory complexes requires the membrane insertases Oxa1 in mitochondria and YidC in bacteria. Oxa1 is responsible for the insertion of the mitochondrial cytochrome c oxidase subunit II (CoxII)...
  73. ncbi Components required for membrane assembly of newly synthesized K+ channel KcsA
    Annemieke van Dalen
    Department of Biochemistry of Membranes, Centre for Biomembranes and Lipid Enzymology, Institute of Biomembranes, Utrecht University, Utrecht, The Netherlands
    FEBS Lett 511:51-8. 2002
    ..In the absence of YidC or at reduced SecYEG levels, KcsA assembly occurs with lower efficiency...
  74. ncbi Conditional lethal mutations separate the M13 procoat and Pf3 coat functions of YidC: different YIDC structural requirements for membrane protein insertion
    Minyong Chen
    Department of Chemistry and Biochemistry Program, The Ohio State University, Columbus, Ohio 43210, USA
    J Biol Chem 278:23295-300. 2003
    Conditional lethal YidC mutants have been isolated to decipher the role of YidC in the assembly of Sec-dependent and Sec-independent membrane proteins...
  75. pmc The two membrane segments of leader peptidase partition one by one into the lipid bilayer via a Sec/YidC interface
    Edith N G Houben
    Department of Molecular Microbiology, Institute of Molecular Cell Biology, Vrije Universiteit, De Boelelaan 1087, 1081 HV Amsterdam, The Netherlands
    EMBO Rep 5:970-5. 2004
    ..Using photo-crosslinking, the first TM (H1) was shown to insert at a Sec-translocon/YidC interface in a fixed orientation...
  76. doi YidC and Oxa1 form dimeric insertion pores on the translating ribosome
    Rebecca Kohler
    Institute of Molecular Biology and Biophysics, ETH Zurich, Zurich, Switzerland
    Mol Cell 34:344-53. 2009
    The YidC/Oxa1/Alb3 family of membrane proteins facilitates the insertion and assembly of membrane proteins in bacteria, mitochondria, and chloroplasts...
  77. pmc YidC family members are involved in the membrane insertion, lateral integration, folding, and assembly of membrane proteins
    Ross E Dalbey
    Department of Chemistry, The Ohio State University, Columbus, OH 43210, USA
    J Cell Biol 166:769-74. 2004
    Members of the YidC family exist in all three domains of life, where they control the assembly of a large variety of membrane protein complexes that function as transporters, energy devices, or sensor proteins...
  78. ncbi IcsA, a polarly localized autotransporter with an atypical signal peptide, uses the Sec apparatus for secretion, although the Sec apparatus is circumferentially distributed
    Lauren D Brandon
    Division of Infectious Diseases, Massachusetts General Hospital, Cambridge, MA 02139, USA
    Mol Microbiol 50:45-60. 2003
    ..Our data suggest that YidC is not required for IcsA secretion. Furthermore, we show that polar localization of IcsA is independent of SecA...
  79. doi Protein transport across and into cell membranes in bacteria and archaea
    Jijun Yuan
    Department of Chemistry, The Ohio State University, 100 West 18th Avenue, Columbus, OH 43210, USA
    Cell Mol Life Sci 67:179-99. 2010
    In the three domains of life, the Sec, YidC/Oxa1, and Tat translocases play important roles in protein translocation across membranes and membrane protein insertion...
  80. ncbi Biogenesis of inner membrane proteins in Escherichia coli
    Joen Luirink
    Department of Microbiology, Institute of Molecular Cell Biology, Vrije Universiteit, 1081 HV Amsterdam, The Netherlands
    Annu Rev Microbiol 59:329-55. 2005
    ..Recently, YidC has been identified as a factor that assists in the integration, folding, and assembly of IMPs both in association ..
  81. doi The charge distribution in the cytoplasmic loop of subunit C of the F1F0 ATPase is a determinant for YidC targeting
    Stefan Kol
    Department of Molecular Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute and the Zernike Institute of Advanced Materials, University of Groningen, Haren, The Netherlands
    J Biol Chem 283:9871-7. 2008
    b>YidC is a member of the Oxa1 family of proteins that facilitates the membrane insertion of a subset of inner membrane proteins in Escherichia coli...
  82. ncbi Crystal structure of the major periplasmic domain of the bacterial membrane protein assembly facilitator YidC
    David C Oliver
    Department of Molecular Biology and Biochemistry, Simon Fraser University, Burnaby, British Columbia V5A 1S6, Canada
    J Biol Chem 283:5208-16. 2008
    The essential bacterial membrane protein YidC facilitates insertion and assembly of proteins destined for integration into the inner membrane. It has homologues in both mitochondria and chloroplasts...
  83. ncbi YidC--an evolutionary conserved device for the assembly of energy-transducing membrane protein complexes
    Martin van der Laan
    Institut fur Biochemie und Molekularbiologie, Universitat Freiburg, Hermann Herder Strasse 7, D 79104 Freiburg, Germany
    Curr Opin Microbiol 8:182-7. 2005
    Members of the YidC/Oxa1/Alb3 membrane protein family are multifunctional mediators of membrane protein integration, folding and assembly into large complexes...
  84. ncbi YidC-mediated membrane insertion of assembly mutants of subunit c of the F1F0 ATPase
    Stefan Kol
    Department of Molecular Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute and the Materials Science Center Plus, University of Groningen, 9751 NN Haren, The Netherlands
    J Biol Chem 281:29762-8. 2006
    b>YidC is a member of the OxaI family of membrane proteins that has been implicated in the membrane insertion of inner membrane proteins in Escherichia coli...

Research Grants3

  1. Protein Transport Across Cell Membranes Gordon Conference 2005
    Ross Dalbey; Fiscal Year: 2005
    ..abstract_text> ..
  2. Molecular analysis of the dimeric F1/F0-ATP synthase
    Rosemary Stuart; Fiscal Year: 2006
    ....
  3. Inner Membrane Protein Assembly in Bacteria
    Ross Dalbey; Fiscal Year: 2009
    ..These proteins require a novel protein called YidC, which was discovered in the year 2000...