wza

Summary

Gene Symbol: wza
Description: colanic acid export protein; outer membrane auxillary lipoprotein
Alias: ECK2056, JW2047
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. ncbi Crystallization and preliminary X-ray diffraction analysis of Wza outer-membrane lipoprotein from Escherichia coli serotype O9a:K30
    Konstantinos Beis
    Centre for Biomolecular Sciences, University of St Andrews, North Haugh, St Andrews, Fife KY16 9ST, Scotland
    Acta Crystallogr D Biol Crystallogr 60:558-60. 2004
  2. pmc The 3D structure of a periplasm-spanning platform required for assembly of group 1 capsular polysaccharides in Escherichia coli
    Richard F Collins
    Faculty of Life Science, University of Manchester, Manchester M60 1QD, United Kingdom
    Proc Natl Acad Sci U S A 104:2390-5. 2007
  3. ncbi Gene products required for surface expression of the capsular form of the group 1 K antigen in Escherichia coli (O9a:K30)
    J Drummelsmith
    Department of Microbiology, University of Guelph, Ontario, Canada
    Mol Microbiol 31:1321-32. 1999
  4. pmc Translocation of group 1 capsular polysaccharide to the surface of Escherichia coli requires a multimeric complex in the outer membrane
    J Drummelsmith
    Department of Microbiology, University of Guelph, Guelph, Ontario, Canada N1G 2W1
    EMBO J 19:57-66. 2000
  5. ncbi Translocation of group 1 capsular polysaccharide in Escherichia coli serotype K30. Structural and functional analysis of the outer membrane lipoprotein Wza
    Jutta Nesper
    Department of Microbiology, University of Guelph, Guelph, Ontario N1G2W1, Canada
    J Biol Chem 278:49763-72. 2003
  6. ncbi Three-dimensional structure of Wza, the protein required for translocation of group 1 capsular polysaccharide across the outer membrane of Escherichia coli
    Konstantinos Beis
    Centre for Biomolecular Sciences, University of St Andrews, North Haugh, St Andrews, Fife KY16 9ST, Scotland, United Kingdom
    J Biol Chem 279:28227-32. 2004
  7. pmc PELDOR spectroscopy distance fingerprinting of the octameric outer-membrane protein Wza from Escherichia coli
    Gregor Hagelueken
    Centre for Biomolecular Sciences, The University of St Andrews, Fife KY16 9RH, UK
    Angew Chem Int Ed Engl 48:2904-6. 2009
  8. pmc Organization of the Escherichia coli K-12 gene cluster responsible for production of the extracellular polysaccharide colanic acid
    G Stevenson
    Department of Microbiology, University of Sydney, New South Wales, Australia
    J Bacteriol 178:4885-93. 1996
  9. pmc Structure-function relationships of the outer membrane translocon Wza investigated by cryo-electron microscopy and mutagenesis
    Robert C Ford
    Manchester Interdisciplinary Biocentre, Faculty of Life Sciences, University of Manchester, Manchester M1 7DN, UK
    J Struct Biol 166:172-82. 2009
  10. ncbi Wza: a new structural paradigm for outer membrane secretory proteins?
    Richard F Collins
    Faculty of Engineering and Physical Sciences, Manchester Interdisciplinary Biocentre, The University of Manchester, Manchester, M1 7DN, UK
    Trends Microbiol 15:96-100. 2007

Scientific Experts

  • Konstantinos Beis
  • Robert C Ford
  • C Whitfield
  • Richard F Collins
  • James H Naismith
  • Changjiang Dong
  • Jutta Nesper
  • J Drummelsmith
  • Gregor Hagelueken
  • Bradley R Clarke
  • Bong Hyun Sung
  • Anne N Reid
  • M Wehland
  • W John Ingledew
  • Hassane ElMkami
  • Biljana Petrovic-Stojanovska
  • Hexian Huang
  • Olav Schiemann
  • W Ebel
  • Jeremy P Derrick
  • Catherine H Botting
  • Catherine McDonnell
  • Byung Jo Yu
  • Ju Young Lee
  • Frederick R Blattner
  • Jun Hyoung Lee
  • Mi Sun Kim
  • Sun Chang Kim
  • Choong Hoon Lee
  • Anne L Brunkan-Lamontagne
  • I T Paulsen
  • Anne Paiment
  • George Harauz
  • Chris M D Hill
  • G Stevenson
  • V Stout
  • F Bernhard
  • J E Trempy
  • M H Saier
  • A M Beness
  • K Andrianopoulos
  • M Hobbs
  • P R Reeves

Detail Information

Publications19

  1. ncbi Crystallization and preliminary X-ray diffraction analysis of Wza outer-membrane lipoprotein from Escherichia coli serotype O9a:K30
    Konstantinos Beis
    Centre for Biomolecular Sciences, University of St Andrews, North Haugh, St Andrews, Fife KY16 9ST, Scotland
    Acta Crystallogr D Biol Crystallogr 60:558-60. 2004
    A novel integral membrane lipoprotein, Wza, from Escherichia coli serotype O9a:K30 has been purified and crystallized. Wza is required for the surface expression of the serotype K30 group 1 capsular polysaccharide of E...
  2. pmc The 3D structure of a periplasm-spanning platform required for assembly of group 1 capsular polysaccharides in Escherichia coli
    Richard F Collins
    Faculty of Life Science, University of Manchester, Manchester M60 1QD, United Kingdom
    Proc Natl Acad Sci U S A 104:2390-5. 2007
    ..Group 1 CPS export requires oligomers of the outer membrane protein, Wza, for translocation across the outer membrane...
  3. ncbi Gene products required for surface expression of the capsular form of the group 1 K antigen in Escherichia coli (O9a:K30)
    J Drummelsmith
    Department of Microbiology, University of Guelph, Ontario, Canada
    Mol Microbiol 31:1321-32. 1999
    ..Three additional genes, wza, wzb and wzc, encode homologues to proteins that are encoded by gene clusters involved in expression of a variety ..
  4. pmc Translocation of group 1 capsular polysaccharide to the surface of Escherichia coli requires a multimeric complex in the outer membrane
    J Drummelsmith
    Department of Microbiology, University of Guelph, Guelph, Ontario, Canada N1G 2W1
    EMBO J 19:57-66. 2000
    Surface expression of the group 1 K30 capsular polysaccharide of Escherichia coli strain E69 (O9a:K30) requires Wza(K30), a member of the outer membrane auxiliary (OMA) protein family...
  5. ncbi Translocation of group 1 capsular polysaccharide in Escherichia coli serotype K30. Structural and functional analysis of the outer membrane lipoprotein Wza
    Jutta Nesper
    Department of Microbiology, University of Guelph, Guelph, Ontario N1G2W1, Canada
    J Biol Chem 278:49763-72. 2003
    ..The Wza protein was shown previously to be required for the formation of the prototype group 1 capsule structure on the ..
  6. ncbi Three-dimensional structure of Wza, the protein required for translocation of group 1 capsular polysaccharide across the outer membrane of Escherichia coli
    Konstantinos Beis
    Centre for Biomolecular Sciences, University of St Andrews, North Haugh, St Andrews, Fife KY16 9ST, Scotland, United Kingdom
    J Biol Chem 279:28227-32. 2004
    b>Wza is a highly conserved multimeric outer membrane protein complex required for the surface expression of the serotype K30 group 1 capsular polysaccharide in Escherichia coli...
  7. pmc PELDOR spectroscopy distance fingerprinting of the octameric outer-membrane protein Wza from Escherichia coli
    Gregor Hagelueken
    Centre for Biomolecular Sciences, The University of St Andrews, Fife KY16 9RH, UK
    Angew Chem Int Ed Engl 48:2904-6. 2009
    ..electron-electron double resonance spectroscopy (PELDOR) is applied to the octameric membrane protein complex Wza of E. coli...
  8. pmc Organization of the Escherichia coli K-12 gene cluster responsible for production of the extracellular polysaccharide colanic acid
    G Stevenson
    Department of Microbiology, University of Sydney, New South Wales, Australia
    J Bacteriol 178:4885-93. 1996
    ..Since homologs of these genes are found in other extracellular polysaccharide gene clusters, they may have a common function, such as export of polysaccharide from the cell...
  9. pmc Structure-function relationships of the outer membrane translocon Wza investigated by cryo-electron microscopy and mutagenesis
    Robert C Ford
    Manchester Interdisciplinary Biocentre, Faculty of Life Sciences, University of Manchester, Manchester M1 7DN, UK
    J Struct Biol 166:172-82. 2009
    The outer membrane protein Wza, from Escherichia coli K30, forms an octameric complex that is essential for capsular polysaccharide export...
  10. ncbi Wza: a new structural paradigm for outer membrane secretory proteins?
    Richard F Collins
    Faculty of Engineering and Physical Sciences, Manchester Interdisciplinary Biocentre, The University of Manchester, Manchester, M1 7DN, UK
    Trends Microbiol 15:96-100. 2007
    ..coli, the passage of the group 1 capsular polysaccharide is mediated by an integral outer membrane protein, Wza. The crystal structure of Wza, determined recently, reveals a novel transmembrane alpha-helical barrel and a large ..
  11. pmc Wza the translocon for E. coli capsular polysaccharides defines a new class of membrane protein
    Changjiang Dong
    Centre for Biomolecular Sciences, The University of St Andrews, Fife KY16 9RH, UK
    Nature 444:226-9. 2006
    ..Here we describe the 2.26 A resolution structure of the 340 kDa octamer of Wza, an integral outer membrane lipoprotein, which is essential for group 1 capsule export in Escherichia coli...
  12. pmc Two-step purification of outer membrane proteins
    Konstantinos Beis
    Centre for Biomolecular Sciences, University of St Andrews, St Andrews, Fife KY16 9ST, UK
    Int J Biol Macromol 39:10-4. 2006
    ..We have tested this protocol for the purification of Wza and Osmoporin C (OmpC) proteins...
  13. pmc functional analysis of conserved gene products involved in assembly of Escherichia coli capsules and exopolysaccharides: evidence for molecular recognition between Wza and Wzc for colanic acid biosynthesis
    Anne N Reid
    Department of Molecular and Cellular Biology, New Science Complex, University of Guelph, Guelph, Ontario, Canada N1G 2W1
    J Bacteriol 187:5470-81. 2005
    ..In this biosynthesis pathway, Wza, Wzb, and Wzc homologues are required for surface expression of wild-type CPS or EPS...
  14. ncbi The RcsAB box. Characterization of a new operator essential for the regulation of exopolysaccharide biosynthesis in enteric bacteria
    M Wehland
    Freie Universitat Berlin, Institut fur Kristallographie, Takustrasse 6, D 14195 Berlin, Germany
    J Biol Chem 275:7013-20. 2000
    ..We describe RcsAB binding sites in the wza promoter of the operon for colanic acid biosynthesis in Escherichia coli K-12, in the galF promoter of the operon ..
  15. pmc Escherichia coli RcsA, a positive activator of colanic acid capsular polysaccharide synthesis, functions To activate its own expression
    W Ebel
    Department of Microbiology, Oregon State University, Corvallis, Oregon 97331 3804, USA
    J Bacteriol 181:577-84. 1999
    ..coli cps promoter and the Erwinia amylovora ams promoter and previously shown to be the RcsA-RcsB binding site, was identified in the rcsA promoter region and shown to be required for high-level expression of rcsA...
  16. ncbi Computer-based analyses of the protein constituents of transport systems catalysing export of complex carbohydrates in bacteria
    I T Paulsen
    Department of Biology, University of California at San Diego, La Jolla 92093 0116, USA
    Microbiology 143:2685-99. 1997
    ..Each family is characterized phylogenetically...
  17. pmc Identification of the promoter region for the colanic acid polysaccharide biosynthetic genes in Escherichia coli K-12
    V Stout
    Department of Microbiology and Molecular and Cellular Biology Graduate Program, Arizona State University, Tempe, Arizona 85287 2701, USA
    J Bacteriol 178:4273-80. 1996
    ....
  18. ncbi Biosynthesis and assembly of capsular polysaccharides in Escherichia coli
    Chris Whitfield
    Department of Molecular and Cellular Biology, University of Guelph, Guelph, Ontario N1G 2W1, Canada
    Annu Rev Biochem 75:39-68. 2006
    ..These systems have an impact on both a general understanding of membrane trafficking in bacteria and on bacterial pathogenesis...
  19. pmc Development of a biofilm production-deficient Escherichia coli strain as a host for biotechnological applications
    Bong Hyun Sung
    Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 373 1 Guseong dong Yuseong gu, Daejeon 305 701, Korea
    Appl Environ Microbiol 72:3336-42. 2006
    ..involved in curli biosynthesis and assembly, Delta(csgG-csgC); colanic acid biosynthesis and assembly, Delta(wcaL-wza); and type I pilus biosynthesis, Delta(fimB-fimH). E...