rseP

Summary

Gene Symbol: rseP
Description: inner membrane zinc RIP metalloprotease; RpoE activator, by degrading RseA; cleaved signal peptide endoprotease
Alias: ECK0175, JW0171, ecfE, yaeL
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. pmc Roles of the membrane-reentrant β-hairpin-like loop of RseP protease in selective substrate cleavage
    Koichiro Akiyama
    Institute for Virus Research, Kyoto University, Kyoto, Japan
    elife 4:. 2015
  2. doi PDZ domains of RseP are not essential for sequential cleavage of RseA or stress-induced σ(E) activation in vivo
    Yohei Hizukuri
    Institute for Virus Research, Kyoto University, Kyoto 606 8507, Japan
    Mol Microbiol 86:1232-45. 2012
  3. pmc Modulating substrate choice: the SspB adaptor delivers a regulator of the extracytoplasmic-stress response to the AAA+ protease ClpXP for degradation
    Julia M Flynn
    Department of Biology and Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Genes Dev 18:2292-301. 2004
  4. pmc Post-liberation cleavage of signal peptides is catalyzed by the site-2 protease (S2P) in bacteria
    Akira Saito
    Institute for Virus Research, Kyoto University, Kyoto 606 8507, Japan
    Proc Natl Acad Sci U S A 108:13740-5. 2011
  5. pmc Cleavage of RseA by RseP requires a carboxyl-terminal hydrophobic amino acid following DegS cleavage
    Xiaochun Li
    Ministry of Education Protein Science Laboratory and Center for Structural Biology, Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, China
    Proc Natl Acad Sci U S A 106:14837-42. 2009
  6. pmc Regulation of ribonuclease E activity by the L4 ribosomal protein of Escherichia coli
    Dharam Singh
    Institute of Molecular Biology, Academia Sinica, Taipei 11529, Taiwan
    Proc Natl Acad Sci U S A 106:864-9. 2009
  7. pmc A pair of circularly permutated PDZ domains control RseP, the S2P family intramembrane protease of Escherichia coli
    Kenji Inaba
    Medical Institute of Bioregulation, Kyushu University, Fukuoka 812 8582, Japan
    J Biol Chem 283:35042-52. 2008
  8. doi Substrate recognition and binding by RseP, an Escherichia coli intramembrane protease
    Kayo Koide
    Institute for Virus Research, Kyoto University, Kawara chu, Shogoin, Kyoto 606 8507, Japan
    J Biol Chem 283:9562-70. 2008
  9. pmc Visualizing the proteome of Escherichia coli: an efficient and versatile method for labeling chromosomal coding DNA sequences (CDSs) with fluorescent protein genes
    Rory M Watt
    Open Laboratory of Chemical Biology, The Institute of Molecular Technology for Drug Discovery and Synthesis, Department of Chemistry, The University of Hong Kong, Hong Kong SAR, China
    Nucleic Acids Res 35:e37. 2007
  10. ncbi Environment of the active site region of RseP, an Escherichia coli regulated intramembrane proteolysis protease, assessed by site-directed cysteine alkylation
    Kayo Koide
    Institute for Virus Research, Kyoto University, Kyoto 606 8507, Japan
    J Biol Chem 282:4553-60. 2007

Scientific Experts

  • Kazue Kanehara
  • Eugene Kolker
  • Yoshinori Akiyama
  • Yohei Hizukuri
  • Koreaki Ito
  • Kayo Koide
  • Benjamin M Alba
  • Koichiro Akiyama
  • Hiroyuki Mori
  • Chantal Bohn
  • Akira Saito
  • Carol A Gross
  • Xiaochun Li
  • Dharam Singh
  • Kenji Inaba
  • Rory M Watt
  • Véronique Douchin
  • Philippe Bouloc
  • Julia M Flynn
  • Irina L Grigorova
  • Terukazu Nogi
  • Shinya Mizuno
  • David Drew
  • C Dartigalongue
  • Osamu Nishimura
  • Shinobu Chiba
  • Ei ichi Matsuo
  • M S Brown
  • Boyuan Wang
  • Jiawei Wang
  • Olga V Averina
  • Vladimir R Kaberdin
  • Lihui Feng
  • Pei Hsun Lin
  • Ssu Jean Chang
  • Hui Kang
  • Yang Qi
  • Sue Lin-Chao
  • Yigong Shi
  • Shuji Akiyama
  • Mamoru Suzuki
  • D Z Rudner
  • Ken ichi Maegawa
  • Kathryn S E Cheah
  • Jing Wang
  • Jian Dong Huang
  • Meikid Leong
  • Depei Liu
  • Hsiang Fu Kung
  • Antoine Danchin
  • Saki Maegawa
  • Justine Collier
  • Hong Ji Zhong
  • Virgil Rhodius
  • Rachna Chaba
  • Robert T Sauer
  • Igor Levchenko
  • Tania A Baker
  • Christophe Herman
  • Chen Ni Chin
  • Gunnar von Heijne
  • Christina Onufryk
  • Jennifer A Leeds
  • Dan Sjöstrand
  • Johan Nilsson
  • Thomas Urbig
  • Jan Willem de Gier
  • Chi Zen Lu
  • S Raina
  • H Loferer
  • R B Rawson
  • J Ye
  • J L Goldstein
  • R Losick
  • P Fawcett
  • T Icho
  • C P Sparrow
  • C R Raetz

Detail Information

Publications27

  1. pmc Roles of the membrane-reentrant β-hairpin-like loop of RseP protease in selective substrate cleavage
    Koichiro Akiyama
    Institute for Virus Research, Kyoto University, Kyoto, Japan
    elife 4:. 2015
    Molecular mechanisms underlying substrate recognition and cleavage by Escherichia coli RseP, which belongs to S2P family of intramembrane-cleaving proteases, remain unclear...
  2. doi PDZ domains of RseP are not essential for sequential cleavage of RseA or stress-induced σ(E) activation in vivo
    Yohei Hizukuri
    Institute for Virus Research, Kyoto University, Kyoto 606 8507, Japan
    Mol Microbiol 86:1232-45. 2012
    ..Stress cues activate DegS to cleave RseA; a second cleavage by RseP releases RseA from the membrane, enabling its rapid degradation...
  3. pmc Modulating substrate choice: the SspB adaptor delivers a regulator of the extracytoplasmic-stress response to the AAA+ protease ClpXP for degradation
    Julia M Flynn
    Department of Biology and Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Genes Dev 18:2292-301. 2004
    ..Thus, these experiments elucidate the final steps in induction of the extracytoplasmic stress response and reveal that SspB delivers a broader spectrum of substrates to ClpXP than has been recognized...
  4. pmc Post-liberation cleavage of signal peptides is catalyzed by the site-2 protease (S2P) in bacteria
    Akira Saito
    Institute for Virus Research, Kyoto University, Kyoto 606 8507, Japan
    Proc Natl Acad Sci U S A 108:13740-5. 2011
    ..Here we show that Escherichia coli RseP, an site-2 protease (S2P) family I-CLiP, introduces a cleavage into SPs after their signal peptidase-mediated ..
  5. pmc Cleavage of RseA by RseP requires a carboxyl-terminal hydrophobic amino acid following DegS cleavage
    Xiaochun Li
    Ministry of Education Protein Science Laboratory and Center for Structural Biology, Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, China
    Proc Natl Acad Sci U S A 106:14837-42. 2009
    ..reconstituted sequential, in vitro cleavages of the Escherichia coli transmembrane protein RseA by DegS (S1P) and RseP (S2P)...
  6. pmc Regulation of ribonuclease E activity by the L4 ribosomal protein of Escherichia coli
    Dharam Singh
    Institute of Molecular Biology, Academia Sinica, Taipei 11529, Taiwan
    Proc Natl Acad Sci U S A 106:864-9. 2009
    ..revealed changes in the abundance of 65 mRNAs encoding the stress response proteins HslO, Lon, CstA, YjiY, and YaeL, as well as proteins involved in carbohydrate and amino acid metabolism and transport, transcription/translation, ..
  7. pmc A pair of circularly permutated PDZ domains control RseP, the S2P family intramembrane protease of Escherichia coli
    Kenji Inaba
    Medical Institute of Bioregulation, Kyushu University, Fukuoka 812 8582, Japan
    J Biol Chem 283:35042-52. 2008
    ..coli is activated through sequential cleavages of the anti-sigma(E) protein, RseA, by membrane proteases DegS and RseP. Without the first cleavage by DegS, RseP is unable to cleave full-length RseA...
  8. doi Substrate recognition and binding by RseP, an Escherichia coli intramembrane protease
    Kayo Koide
    Institute for Virus Research, Kyoto University, Kawara chu, Shogoin, Kyoto 606 8507, Japan
    J Biol Chem 283:9562-70. 2008
    Escherichia coli RseP belongs to the S2P family of intramembrane cleaving proteases...
  9. pmc Visualizing the proteome of Escherichia coli: an efficient and versatile method for labeling chromosomal coding DNA sequences (CDSs) with fluorescent protein genes
    Rory M Watt
    Open Laboratory of Chemical Biology, The Institute of Molecular Technology for Drug Discovery and Synthesis, Department of Chemistry, The University of Hong Kong, Hong Kong SAR, China
    Nucleic Acids Res 35:e37. 2007
    ..Our results show that E. coli has an organized and dynamic proteome, and demonstrate that this approach is applicable for tagging and (co-) localizing CDSs on a genome-wide scale...
  10. ncbi Environment of the active site region of RseP, an Escherichia coli regulated intramembrane proteolysis protease, assessed by site-directed cysteine alkylation
    Kayo Koide
    Institute for Virus Research, Kyoto University, Kyoto 606 8507, Japan
    J Biol Chem 282:4553-60. 2007
    ..Here we studied the environment surrounding the active site region of RseP, an Escherichia coli S2P ortholog involved in the sigma(E) pathway of extracytoplasmic stress responses...
  11. ncbi Down-regulation of porins by a small RNA bypasses the essentiality of the regulated intramembrane proteolysis protease RseP in Escherichia coli
    Véronique Douchin
    Signalisation et Réseaux de Régulations Bactériens, Institut de Genetique et Microbiologie, CNRS UMR8621 IFR115, Centre Scientifique d Orsay, Universite Paris Sud, 91405 Orsay Cedex, France
    J Biol Chem 281:12253-9. 2006
    ..SigmaE is released in response to stress through the successive RseA cleavage by DegS and the RIP protease RseP. SigmaE and proteases that free it from RseA are essential...
  12. pmc Fine-tuning of the Escherichia coli sigmaE envelope stress response relies on multiple mechanisms to inhibit signal-independent proteolysis of the transmembrane anti-sigma factor, RseA
    Irina L Grigorova
    Graduate Group in Biophysics, University of California, San Francisco, San Francisco, California 94143, USA
    Genes Dev 18:2686-97. 2004
    ..the membrane localized DegS protease cleaves RseA in its periplasmic domain, the membrane-embedded protease RseP (YaeL) cleaves RseA near the inner membrane, and the released cytoplasmic RseA fragment is further degraded...
  13. pmc RseP (YaeL), an Escherichia coli RIP protease, cleaves transmembrane sequences
    Yoshinori Akiyama
    Institute for Virus Research, Kyoto University, Kyoto, Japan
    EMBO J 23:4434-42. 2004
    Escherichia coli RseP (formerly YaeL) is believed to function as a 'regulated intramembrane proteolysis' (RIP) protease that introduces the second cleavage into anti-sigma(E) protein RseA at a position within or close to the transmembrane ..
  14. ncbi Molecular cloning and sequencing of the gene for CDP-diglyceride synthetase of Escherichia coli
    T Icho
    J Biol Chem 260:12078-83. 1985
    ..P., and Raetz, C.R.H., J. Biol. Chem. 260, 12084-12091). Finally, the molecular cloning work reported herein allows the exact placement of the cds gene on the E. coli genetic map...
  15. ncbi Regulation of the Escherichia coli sigma-dependent envelope stress response
    Benjamin M Alba
    Department of Biochemistry and Biophysics, University of California at San Francisco, San Francisco, CA 94143 2200, USA
    Mol Microbiol 52:613-9. 2004
    ..sigma(E) antisigma factor; RseB, a periplasmic protein that binds to the periplasmic face of RseA; and the DegS and YaeL proteases...
  16. ncbi Dispensable PDZ domain of Escherichia coli YaeL essential protease
    Chantal Bohn
    Laboratoire des Réseaux de Régulations et Biogénèse des Enveloppes Bactériennes, Institut de Genetique et Microbiologie, CNRS UMR 8621, Centre Scientifique d Orsay, Universite Paris Sud, Batiment 400, 91405 Orsay Cedex, France
    Mol Microbiol 52:427-35. 2004
    ..stress activates DegS-dependent cleavage of RseA, rendering RseA sensitive to further degradation by the YaeL protease...
  17. pmc YaeL proteolysis of RseA is controlled by the PDZ domain of YaeL and a Gln-rich region of RseA
    Kazue Kanehara
    Institute for Virus Research, Kyoto University, Kyoto 606 8507, Japan
    EMBO J 22:6389-98. 2003
    ..of RseA by two successive proteolytic events: DegS-catalyzed first cleavage at a periplasmic site followed by YaeL-mediated second proteolysis at an intramembrane region...
  18. ncbi [RIP(regulated intramembrane proteolysis): from bacteria to higher organism]
    Kazue Kanehara
    Tanpakushitsu Kakusan Koso 48:836-41. 2003
  19. pmc DegS and YaeL participate sequentially in the cleavage of RseA to activate the sigma(E)-dependent extracytoplasmic stress response
    Benjamin M Alba
    Department of Biochemistry and Biophysics, University of California at San Francisco, San Francisco, California 94143, USA
    Genes Dev 16:2156-68. 2002
    ..events controlled by the inner membrane-anchored protease DegS and the membrane-embedded metalloprotease YaeL, an ortholog of mammalian Site-2 protease (S2P)...
  20. pmc YaeL (EcfE) activates the sigma(E) pathway of stress response through a site-2 cleavage of anti-sigma(E), RseA
    Kazue Kanehara
    Institute for Virus Research, Kyoto University, Kyoto 606 8507, Japan
    Genes Dev 16:2147-55. 2002
    Escherichia coli YaeL (EcfE) is a homolog of human site-2 protease (S2P), a membrane-bound zinc metalloprotease involved in regulated intramembrane proteolysis...
  21. pmc Rapid topology mapping of Escherichia coli inner-membrane proteins by prediction and PhoA/GFP fusion analysis
    David Drew
    Department of Biochemistry and Biophysics, Stockholm University, SE 106 91 Stockholm, Sweden
    Proc Natl Acad Sci U S A 99:2690-5. 2002
    ..We derive new topology models for 12 inner-membrane proteins: MarC, PstA, TatC, YaeL, YcbM, YddQ, YdgE, YedZ, YgjV, YiaB, YigG, and YnfA...
  22. ncbi Characterization of the yaeL gene product and its S2P-protease motifs in Escherichia coli
    K Kanehara
    Institute for Virus Research, Kyoto University, Sakyo ku, Kyoto 606 8507, Japan
    Gene 281:71-9. 2001
    An Escherichia coli open reading frame, yaeL, encodes a predicted homolog of human site-2 protease (S2P), a putative membrane-bound zinc metalloproteinase involved in the proteolytic activation of regulatory factors for sterol ..
  23. pmc EcfE, a new essential inner membrane protease: its role in the regulation of heat shock response in Escherichia coli
    C Dartigalongue
    Departement de Biochimie Medicale, Centre Medical Universitaire, 1 rue Michel Servet 1211 Genève 4, Switzerland
    EMBO J 20:5908-18. 2001
    ..This protease is anchored in the inner membrane and the gene encoding it has been named ecfE, since it is transcribed by Esigma(E) polymerase...
  24. ncbi Regulated intramembrane proteolysis: a control mechanism conserved from bacteria to humans
    M S Brown
    Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas 75390 9046, USA
    Cell 100:391-8. 2000
  25. pmc A family of membrane-embedded metalloproteases involved in regulated proteolysis of membrane-associated transcription factors
    D Z Rudner
    Department of Molecular Biology, Harvard University, 16 Divinity Avenue, Cambridge, MA 02138, USA
    Proc Natl Acad Sci U S A 96:14765-70. 1999
    ..Thus, the pathways that govern the activation of the prokaryotic transcription factor pro-sigma(K) and the mammalian transcription factor SREBP not only are analogous but also use processing enzymes with strikingly homologous features...
  26. pmc Identification and functional analysis of 'hypothetical' genes expressed in Haemophilus influenzae
    Eugene Kolker
    BIATECH, 19310 North Creek Parkway, Suite 115, Bothell, WA 98011, USA
    Nucleic Acids Res 32:2353-61. 2004
    ....