rpoS

Summary

Gene Symbol: rpoS
Description: RNA polymerase, sigma S (sigma 38) factor
Alias: ECK2736, JW5437, abrD, appR, csi2, dpeB, katF, nur, otsX, sigS
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. ncbi Expression of Escherichia coli pyruvate oxidase (PoxB) depends on the sigma factor encoded by the rpoS(katF) gene
    Y Y Chang
    Department of Microbiology, University of Illinois, Urbana 61801
    Mol Microbiol 11:1019-28. 1994
  2. pmc Sequences in the -35 region of Escherichia coli rpoS-dependent genes promote transcription by E sigma S
    A Wise
    Section of Microbiology, University of California, Davis 95616, USA
    J Bacteriol 178:2785-93. 1996
  3. ncbi Negative regulation by RpoS: a case of sigma factor competition
    A Farewell
    Department of Microbiology, Lund University, Sweden
    Mol Microbiol 29:1039-51. 1998
  4. pmc Regulation of RpoS proteolysis in Escherichia coli: the response regulator RssB is a recognition factor that interacts with the turnover element in RpoS
    G Becker
    Institute of Plant Physiology and Microbiology, Free University of Berlin, 14195 Berlin, Germany
    Proc Natl Acad Sci U S A 96:6439-44. 1999
  5. ncbi What makes an Escherichia coli promoter sigma(S) dependent? Role of the -13/-14 nucleotide promoter positions and region 2.5 of sigma(S)
    G Becker
    Institut für Biologie Mikrobiologie, Freie Universitat Berlin, Königin Luise Str 12 16, 14195 Berlin, Germany
    Mol Microbiol 39:1153-65. 2001
  6. ncbi Promoter recognition and discrimination by EsigmaS RNA polymerase
    T Gaal
    Department of Bacteriology, University of Wisconsin, Madison WI 53706, USA
    Mol Microbiol 42:939-54. 2001
  7. pmc An N-terminally truncated RpoS (sigma(S)) protein in Escherichia coli is active in vivo and exhibits normal environmental regulation even in the absence of rpoS transcriptional and translational control signals
    K Rajkumari
    Centre for Cellular and Molecular Biology, Hyderabad 500 007, India
    J Bacteriol 184:3167-75. 2002
  8. pmc Signal transduction and regulatory mechanisms involved in control of the sigma(S) (RpoS) subunit of RNA polymerase
    Regine Hengge-Aronis
    Institut fur Biologie, Mikrobiologie, Freie Universitat Berlin, 14195 Berlin, Germany
    Microbiol Mol Biol Rev 66:373-95, table of contents. 2002
  9. ncbi Regulation and mode of action of the second small RNA activator of RpoS translation, RprA
    Nadim Majdalani
    Laboratory of Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892 4264, USA
    Mol Microbiol 46:813-26. 2002
  10. ncbi Interactions of the non-coding RNA DsrA and RpoS mRNA with the 30 S ribosomal subunit
    David J Worhunsky
    Department of Chemistry and Program in Biological Chemistry, Bates College, Lewiston, Maine 04240, USA
    J Biol Chem 278:15815-24. 2003

Research Grants

Scientific Experts

Detail Information

Publications111 found, 100 shown here

  1. ncbi Expression of Escherichia coli pyruvate oxidase (PoxB) depends on the sigma factor encoded by the rpoS(katF) gene
    Y Y Chang
    Department of Microbiology, University of Illinois, Urbana 61801
    Mol Microbiol 11:1019-28. 1994
    ..We report that PoxB activity is dependent on a functional rpoS(katF) gene which encodes a sigma factor required to transcribe a number of stationary-phase-induced genes...
  2. pmc Sequences in the -35 region of Escherichia coli rpoS-dependent genes promote transcription by E sigma S
    A Wise
    Section of Microbiology, University of California, Davis 95616, USA
    J Bacteriol 178:2785-93. 1996
    ..Our data suggest that DNA sequences in the -35 region function as part of a discriminator mechanism to shift transcription between E sigma D and E sigma S...
  3. ncbi Negative regulation by RpoS: a case of sigma factor competition
    A Farewell
    Department of Microbiology, Lund University, Sweden
    Mol Microbiol 29:1039-51. 1998
    A mutation in the Escherichia coli gene encoding the stationary phase-inducible sigma factor (sigmaS, RpoS) not only abolishes transcription of some genes in stationary phase, but also causes superinduction of other stationary phase-..
  4. pmc Regulation of RpoS proteolysis in Escherichia coli: the response regulator RssB is a recognition factor that interacts with the turnover element in RpoS
    G Becker
    Institute of Plant Physiology and Microbiology, Free University of Berlin, 14195 Berlin, Germany
    Proc Natl Acad Sci U S A 96:6439-44. 1999
    The degradation of the RpoS (sigmaS) subunit of RNA polymerase in Escherichia coli is a prime example of regulated proteolysis in prokaryotes...
  5. ncbi What makes an Escherichia coli promoter sigma(S) dependent? Role of the -13/-14 nucleotide promoter positions and region 2.5 of sigma(S)
    G Becker
    Institut für Biologie Mikrobiologie, Freie Universitat Berlin, Königin Luise Str 12 16, 14195 Berlin, Germany
    Mol Microbiol 39:1153-65. 2001
    ..5 in sigmaS in transcription initiation. Moreover, we propose a consensus sequence for a sigmaS-selective promoter and discuss its emergence and functional properties from an evolutionary point of view...
  6. ncbi Promoter recognition and discrimination by EsigmaS RNA polymerase
    T Gaal
    Department of Bacteriology, University of Wisconsin, Madison WI 53706, USA
    Mol Microbiol 42:939-54. 2001
    ..DNA recognition by EsigmaS versus Esigma70 thus presents an alternative solution to the problem of promoter selectivity...
  7. pmc An N-terminally truncated RpoS (sigma(S)) protein in Escherichia coli is active in vivo and exhibits normal environmental regulation even in the absence of rpoS transcriptional and translational control signals
    K Rajkumari
    Centre for Cellular and Molecular Biology, Hyderabad 500 007, India
    J Bacteriol 184:3167-75. 2002
    b>RpoS (sigma(S)) in Escherichia coli is a stationary-phase-specific primary sigma factor of RNA polymerase which is 330 amino acids long and belongs to the eubacterial sigma(70) family of proteins. Conserved domain 1...
  8. pmc Signal transduction and regulatory mechanisms involved in control of the sigma(S) (RpoS) subunit of RNA polymerase
    Regine Hengge-Aronis
    Institut fur Biologie, Mikrobiologie, Freie Universitat Berlin, 14195 Berlin, Germany
    Microbiol Mol Biol Rev 66:373-95, table of contents. 2002
    The sigma(S) (RpoS) subunit of RNA polymerase is the master regulator of the general stress response in Escherichia coli and related bacteria...
  9. ncbi Regulation and mode of action of the second small RNA activator of RpoS translation, RprA
    Nadim Majdalani
    Laboratory of Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892 4264, USA
    Mol Microbiol 46:813-26. 2002
    Translation of the stationary phase sigma factor RpoS is stimulated by at least two small RNAs, DsrA and RprA. DsrA disrupts an inhibitory secondary structure in the rpoS leader mRNA by pairing with the upstream RNA...
  10. ncbi Interactions of the non-coding RNA DsrA and RpoS mRNA with the 30 S ribosomal subunit
    David J Worhunsky
    Department of Chemistry and Program in Biological Chemistry, Bates College, Lewiston, Maine 04240, USA
    J Biol Chem 278:15815-24. 2003
    Expression of sigma(s), the gene product of rpoS, is controlled translationally in response to many environmental stresses...
  11. pmc In vitro properties of RpoS (sigma(S)) mutants of Escherichia coli with postulated N-terminal subregion 1.1 or C-terminal region 4 deleted
    J Gowrishankar
    Laboratory of Bacterial Genetics, Centre for DNA Fingerprinting and Diagnostics, Hyderabad 500 076, India
    J Bacteriol 185:2673-9. 2003
    ..1 of sigma(70), substitutions in which have previously been shown to rectify the anomalous electrophoretic migration of sigma(70) (V. Gopal and D. Chatterji, Eur. J. Biochem. 244:614-618, 1997)...
  12. ncbi Small non-coding RNAs, co-ordinators of adaptation processes in Escherichia coli: the RpoS paradigm
    F Repoila
    UMR960 INRA ENVT, Laboratoire de Microbiologie Moléculaire, 23 Chemin des Capelles, 31076 Toulouse Cedex, France
    Mol Microbiol 48:855-61. 2003
    ..This suggests that they play a role in cell adaptation. In this review, we use the regulation of RpoS (sigma38) translation as a paradigm of sRNA-mediated response to environmental conditions; rpoS is currently the ..
  13. pmc Constitutive activation of the Escherichia coli Pho regulon upregulates rpoS translation in an Hfq-dependent fashion
    Natividad Ruiz
    Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544, USA
    J Bacteriol 185:5984-92. 2003
    Regulation of the sigma factor RpoS occurs at the levels of transcription, translation, and protein stability activity, and it determines whether Escherichia coli turns on or off the stationary-phase response...
  14. ncbi What is driving the acquisition of mutS and rpoS polymorphisms in Escherichia coli?
    Thomas Ferenci
    School of Molecular and Microbial Biosciences G08, University of Sydney, 2006, NSW, Australia
    Trends Microbiol 11:457-61. 2003
    Pathogenic and commensal Escherichia coli isolates frequently contain defective alleles of the mutS and rpoS genes, located in a highly polymorphic segment of the chromosome...
  15. ncbi Escherichia coli rpoS gene has an internal secondary translation initiation region
    Pochi Ramalingam Subbarayan
    Department of Molecular Genetics, National Institute of Genetics, Mishima 411 8540, Shizuoka ken, Japan
    Biochem Biophys Res Commun 313:294-9. 2004
    Sigma S (sigma(s)) encoded by rpoS in Escherichia coli is a stationary phase specific sigma subunit of the RNA polymerase holoenzyme. Widespread among the E. coli K12 strains is an amber mutation that prematurely terminates sigma(s)...
  16. ncbi Escherichia coli evolution during stationary phase
    Erik R Zinser
    Department of Civil and Environmental Engineering, Massachusetts Institute of Technology, 400 Main Street, Cambridge, MA 02139, USA
    Res Microbiol 155:328-36. 2004
    ..Genetic analyses of a single survivor of starvation identified four adaptive mutations(1). Investigation of these mutations has revealed insights into the molecular and physiological bases of evolution during prolonged starvation stress...
  17. pmc A regulatory trade-off as a source of strain variation in the species Escherichia coli
    Thea King
    School of Molecular and Microbial Biosciences G08, The University of Sydney, Sydney, NSW 2006, Australia
    J Bacteriol 186:5614-20. 2004
    ..we report on isolates of Escherichia coli with distinct ratios of sigma factors (RpoD, sigmaD, or sigma70 and RpoS or sigmaS) that affect transcription initiated by RNA polymerase. Both laboratory E...
  18. pmc A stop codon-dependent internal secondary translation initiation region in Escherichia coli rpoS
    Pochi Ramalingam Subbarayan
    Department of Medicine D8 4, University of Miami School of Medicine, 1550 NW 10th Avenue, Fox 431A, FL 33136, USA
    RNA 10:1359-65. 2004
    Sigma S (sigmaS) encoded by rpoS is a stationary phase-specific sigma subunit of the Escherichia coli RNA polymerase holoenzyme. In many E...
  19. pmc SigmaS-dependent gene expression at the onset of stationary phase in Escherichia coli: function of sigmaS-dependent genes and identification of their promoter sequences
    Stephan Lacour
    Swiss Federal Institute of Environmental Technology EAWAG, Dubendorf, Switzerland
    J Bacteriol 186:7186-95. 2004
    The sigma(S) subunit of RNA polymerase, the product of the rpoS gene, controls the expression of genes responding to starvation and cellular stresses...
  20. pmc Escherichia coli Hfq has distinct interaction surfaces for DsrA, rpoS and poly(A) RNAs
    Peter J Mikulecky
    Department of Chemistry, Indiana University, 800 E Kirkwood Avenue, Bloomington, Indiana 47405, USA
    Nat Struct Mol Biol 11:1206-14. 2004
    ..We tested the mutants' DsrA-dependent activation of rpoS, and their ability to stabilize DsrA ncRNA against degradation in vivo...
  21. pmc RpoS-regulated genes of Escherichia coli identified by random lacZ fusion mutagenesis
    Somalinga R V Vijayakumar
    Department of Biology, McMaster University, 1280 Main St West, Hamilton, Ontario, Canada, L8S 4K1
    J Bacteriol 186:8499-507. 2004
    b>RpoS is a conserved alternative sigma factor that regulates the expression of many stress response genes in Escherichia coli. The RpoS regulon is large but has not yet been completely characterized...
  22. pmc Genome-wide analysis of the general stress response network in Escherichia coli: sigmaS-dependent genes, promoters, and sigma factor selectivity
    Harald Weber
    Institut fur Biologie, Mikrobiologie, Freie Universitat Berlin, Königin Luise Str 12 16a, 14195 Berlin, Germany
    J Bacteriol 187:1591-603. 2005
    The sigmaS (or RpoS) subunit of RNA polymerase is the master regulator of the general stress response in Escherichia coli...
  23. ncbi Maintaining a healthy SPANC balance through regulatory and mutational adaptation
    Thomas Ferenci
    School of Molecular and Microbial Biosciences, The University of Sydney, NSW 2006, Australia
    Mol Microbiol 57:1-8. 2005
    ..Porin-mediated outer membrane permeability and the RpoS-controlled general stress response both involve a trade-off between self-preservation and nutritional competence, ..
  24. ncbi Polyamine Modulon in Escherichia coli: genes involved in the stimulation of cell growth by polyamines
    Kazuei Igarashi
    Graduate School of Pharmaceutical Sciences, Chiba University, 1 8 1 Inohana, Chiba 260 8675
    J Biochem 139:11-6. 2006
    ..protein (OppA), which is important for nutrient supply, adenylate cyclase (Cya), RNA polymerase sigma(38) subunit (RpoS), transcription factor of iron transport operon (FecI), and transcription factor of growth-related genes including ..
  25. pmc Genotype-by-environment interactions influencing the emergence of rpoS mutations in Escherichia coli populations
    Thea King
    School of Molecular and Microbial Biosciences, University of Sydney, Sydney, New South Wales 2006, Australia
    Genetics 172:2071-9. 2006
    Polymorphisms in rpoS are common in Escherichia coli. rpoS status influences a trade-off between nutrition and stress resistance and hence fitness across different environments...
  26. ncbi Effect of rpoS gene knockout on the metabolism of Escherichia coli during exponential growth phase and early stationary phase based on gene expressions, enzyme activities and intracellular metabolite concentrations
    Mahbuba Rahman
    Department of Bioscience and Bioinformatics, Kyushu Institute of Technology, Iizuka, Fukuoka 820 8502, Japan
    Biotechnol Bioeng 94:585-95. 2006
    The RNA polymerase sigma factor, encoded by rpoS gene, controls the expression of a large number of genes in Escherichia coli under stress conditions...
  27. pmc Modulating RssB activity: IraP, a novel regulator of sigma(S) stability in Escherichia coli
    Alexandre Bougdour
    Laboratory of Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Genes Dev 20:884-97. 2006
    ..Our results reveal a novel mechanism of regulation of sigma(S) stability through the regulation of RssB activity and identify IraP as a member of a new class of regulators, the anti-adaptor proteins...
  28. ncbi Interactions of ribosomal protein S1 with DsrA and rpoS mRNA
    Rositsa I Koleva
    Center for Molecular Medicine, Maine Medical Center Research Institute, Scarborough, ME 04704, USA
    Biochem Biophys Res Commun 348:662-8. 2006
    Ribosomal protein S1 is shown to interact with the non-coding RNA DsrA and with rpoS mRNA. DsrA is a non-coding RNA that is important in controlling expression of the rpoS gene product in Escherichia coli...
  29. pmc Spectroscopic observation of RNA chaperone activities of Hfq in post-transcriptional regulation by a small non-coding RNA
    Veronique Arluison
    Institut de Biologie Physico Chimique, CNRS UPR 9073 conventionnee avec l université Paris 7, 13 rue P et M Curie, 75005 Paris, France
    Nucleic Acids Res 35:999-1006. 2007
    ..transfer assay to probe how Hfq modulates the interaction between a sRNA, DsrA, and its regulatory target mRNA, rpoS. The relevant RNA fragments were labelled so that changes in intra- and intermolecular RNA structures can be ..
  30. ncbi The molecular basis of selective promoter activation by the sigmaS subunit of RNA polymerase
    Athanasios Typas
    Institut fur Biologie, Mikrobiologie, Freie Universitat Berlin, Königin Luise Str 12 16, 14195 Berlin, Germany
    Mol Microbiol 63:1296-306. 2007
    ..how the principle sigma factor in stationary phase and in stressful conditions in Escherichia coli, sigmaS (RpoS), can specifically target its large regulon in vivo, although it is known to recognize the same core promoter ..
  31. ncbi New insights into the role of sigma factor RpoS as revealed in escherichia coli strains lacking the phosphoenolpyruvate:carbohydrate phosphotransferase system
    Noemí Flores
    Departamento de Ingeniería Celular y Biocatálisis, Instituto de Biotecnologia UNAM, Cuernavaca, Mexico
    J Mol Microbiol Biotechnol 14:176-92. 2008
    It has been demonstrated that about 10% of the Escherichia coli genes are under direct or indirect control of RpoS. Therefore, Weber et al...
  32. ncbi RpoS regulation of gene expression during exponential growth of Escherichia coli K12
    Tao Dong
    Department of Biology, McMaster University, Life Sciences Building, Rm 433, 1280 Main Street West, Hamilton, ON, Canada, L8S 4K1
    Mol Genet Genomics 279:267-77. 2008
    b>RpoS is a major regulator of genes required for adaptation to stationary phase in E. coli...
  33. pmc Translational activation by the noncoding RNA DsrA involves alternative RNase III processing in the rpoS 5'-leader
    Armin Resch
    Max F Perutz Laboratories, Department of Microbiology and Immunobiology, University Departments at the Vienna Biocenter, A 1030 Vienna, Austria
    RNA 14:454-9. 2008
    The intricate regulation of the Escherichia coli rpoS gene, which encodes the stationary phase sigma-factor sigmaS, includes translational activation by the noncoding RNA DsrA...
  34. ncbi Multiple pathways for regulation of sigmaS (RpoS) stability in Escherichia coli via the action of multiple anti-adaptors
    Alexandre Bougdour
    Laboratory of Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Mol Microbiol 68:298-313. 2008
    ..Our results reveal that multiple anti-adaptor proteins allow the regulation of sigmaS stability through the regulation of RssB activity under a variety of stress conditions...
  35. pmc Fur-dependent detoxification of organic acids by rpoS mutants during prolonged incubation under aerobic, phosphate starvation conditions
    Mélanie L Guillemet
    Laboratoire de Chimie Bacterienne, Centre National de la Recherche Scientifique, 13009 Marseille, France
    J Bacteriol 190:5567-75. 2008
    ..We show in this work that rpoS-null mutants incubated in the absence of any amino acid survived during prolonged incubation under aerobic, P(i) ..
  36. pmc The rpoS mRNA leader recruits Hfq to facilitate annealing with DsrA sRNA
    Toby J Soper
    Program in Cellular, Molecular, Developmental Biology and Biophysics, Johns Hopkins University, Baltimore, Maryland 21218 2685, USA
    RNA 14:1907-17. 2008
    ..DsrA sRNA stimulates translation of the RpoS stress response factor in Escherichia coli by base-pairing with the 5' leader of the rpoS mRNA and opening a stem-..
  37. pmc Interplay of cellular cAMP levels, {sigma}S activity and oxidative stress resistance in Escherichia coli
    Evelyn Barth
    Department Chemie, Biotechnologie, Technische Universitat Munchen, Lichtenbergstrasse 4, 85747 Garching, Germany
    Microbiology 155:1680-9. 2009
    ..Downregulation of CRP in turn causes the derepression of rpoS, encoding the alternative sigma factor sigma(S), which activates the general stress response in E. coli...
  38. doi Proteolysis of sigmaS (RpoS) and the general stress response in Escherichia coli
    Regine Hengge
    Institut fur Biologie, Mikrobiologie, Freie Universitat Berlin, Königin Luise Str 12 16, 14195 Berlin, Germany
    Res Microbiol 160:667-76. 2009
    The sigma(S) (RpoS) subunit of RNA polymerase is the master regulator of the general stress response in Escherichia coli. Regulation of sigma(S) occurs at the levels of transcription, translation, proteolysis and protein activity...
  39. pmc Nucleotide sequence of katF of Escherichia coli suggests KatF protein is a novel sigma transcription factor
    M R Mulvey
    Department of Microbiology, University of Manitoba, Winnipeg, Canada
    Nucleic Acids Res 17:9979-91. 1989
    The katF gene of Escherichia coli has been sequenced revealing a 1086 base pair open reading frame from which the sequence of a 362 amino acid protein has been deduced...
  40. pmc A trans-acting RNA as a control switch in Escherichia coli: DsrA modulates function by forming alternative structures
    R A Lease
    Molecular Genetics Program, Wadsworth Center, New York State Department of Health, and School of Public Health, State University of New York at Albany, P O Box 22002, Albany, NY 12201 2002, USA
    Proc Natl Acad Sci U S A 97:9919-24. 2000
    ..regulatory RNA of Escherichia coli that acts in trans by RNA-RNA interactions with two different mRNAs, hns and rpoS. DsrA has opposite effects on these transcriptional regulators...
  41. pmc Regulation of sigma factor competition by the alarmone ppGpp
    Miki Jishage
    Department of Cell and Molecular Biology Microbiology, Goteborg University, 405 30 Göteberg, Sweden
    Genes Dev 16:1260-70. 2002
    ..Thus, the stringent response encompasses a mechanism that alters the relative competitiveness of sigma factors in accordance with cellular demands during physiological stress...
  42. ncbi A comparative study of variation in codon 33 of the rpoS gene in Escherichia coli K12 stocks: implications for the synthesis of sigma(s)
    P R Subbarayan
    Department of Medicine, Division of Hematology and Oncology D8 4, University of Miami School of Medicine, 1550 NW 10th Avenue, Fox 431A, Miami, FL 33136, USA
    Mol Genet Genomics 270:533-8. 2004
    The Escherichia coli rpoS gene encodes an RNA polymerase sigma factor (sigma S or sigma(S)) required for the expression of stationary-phase genes. In the first published rpoS sequence from E. coli K-12 codon 33 is given as CAG...
  43. ncbi Microarray analysis of RpoS-mediated gene expression in Escherichia coli K-12
    C L Patten
    Department of Biology, McMaster University, Hamilton, Ontario, L8S 4K1, Canada
    Mol Genet Genomics 272:580-91. 2004
    The alternative sigma factor RpoS controls the expression of many stationary-phase genes in Escherichia coli and other bacteria...
  44. pmc A DNA damage response in Escherichia coli involving the alternative sigma factor, RpoS
    Houra Merrikh
    Department of Biology and Rosenstiel Basic Medical Sciences Center, Brandeis University, Waltham, MA 02254 9110, USA
    Proc Natl Acad Sci U S A 106:611-6. 2009
    ..are consistent with the function of IraD to promote accumulation of the alternative transcription sigma factor, RpoS, by binding to the adaptor RssB protein that targets RpoS for degradation...
  45. pmc Growth phase and (p)ppGpp control of IraD, a regulator of RpoS stability, in Escherichia coli
    Houra Merrikh
    Department of Biology and Rosenstiel Basic Medical Sciences Research Center, Brandeis University, Waltham, Massachusetts 02454 9110, USA
    J Bacteriol 191:7436-46. 2009
    The antiadaptor protein IraD inhibits the proteolysis of the alternative sigma factor, RpoS, which promotes the synthesis of >100 genes during the general stress response and during stationary phase...
  46. ncbi A comparative study of global stress gene regulation in response to overexpression of recombinant proteins in Escherichia coli
    R T Gill
    Department of Chemical Engineering, University of Maryland, College Park, Maryland 20742, USA
    Metab Eng 2:178-89. 2000
    ..ftsH, clpP, lon, ompT, degP, groEL, aceA, ibpA), SOS/DNA damage (recA, lon, IS5 transposase), stationary phase (rpoS, aceA), and bacteriophage life cycle (ftsH, recA) genes...
  47. pmc rpoS mutations and loss of general stress resistance in Escherichia coli populations as a consequence of conflict between competing stress responses
    Lucinda Notley-McRobb
    Department of Microbiology, University of Sydney, Sydney, New South Wales 2006, Australia
    J Bacteriol 184:806-11. 2002
    The general stress resistance of Escherichia coli is controlled by the RpoS sigma factor (phi(S)), but mutations in rpoS are surprisingly common in natural and laboratory populations...
  48. pmc Signal transduction cascade for regulation of RpoS: temperature regulation of DsrA
    F Repoila
    Laboratory of Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892 4255, USA
    J Bacteriol 183:4012-23. 2001
    Many environmental parameters modulate the amount of the RpoS sigma factor in Escherichia coli. Temperature control of RpoS depends on the untranslated RNA DsrA. DsrA activates RpoS translation by pairing with the leader of the mRNA...
  49. pmc In vivo expression from the RpoS-dependent P1 promoter of the osmotically regulated proU operon in Escherichia coli and Salmonella enterica serovar Typhimurium: activation by rho and hns mutations and by cold stress
    K Rajkumari
    Centre for Cellular and Molecular Biology, Hyderabad 500 007, India
    J Bacteriol 183:6543-50. 2001
    ....
  50. pmc Sigma 32-dependent promoter activity in vivo: sequence determinants of the groE promoter
    Yang Wang
    Department of Biochemistry, Case Western Reserve University, Cleveland, Ohio 44106 4935, USA
    J Bacteriol 185:5800-6. 2003
    ..At each of the specified base pairs, substitutions were found which reduced promoter activity by greater than 75%. Activity was also dependent upon the number of base pairs separating the two regions...
  51. ncbi Positive selection for loss of RpoS function in Escherichia coli
    Guozhu Chen
    Department of Biology, McMaster University, Hamilton, Ont, Canada L8S 4K1
    Mutat Res 554:193-203. 2004
    Though RpoS, an alternative sigma factor, is required for survival and adaptation of Escherichia coli under stress conditions, many strains have acquired independent mutations in the rpoS gene...
  52. ncbi Differential ability of sigma(s) and sigma70 of Escherichia coli to utilize promoters containing half or full UP-element sites
    Athanasios Typas
    Institut für Biologie Mikrobiologie, Freie Universitat Berlin, Königin Luise Str 12 16, 14195 Berlin, Germany
    Mol Microbiol 55:250-60. 2005
    ..Finally, experiments using single amino acid substitutions of sigma(s) indicate that the foundation for this preference lies in an inability of sigma(s) to interact with the a subunit C-terminal domain...
  53. ncbi Electrochemical evaluation of cellular physiological status under stress in Escherichia coli with the rpoS-lacZ reporter gene
    Hisakage Funabashi
    Department of Biological Information, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Tokyo, Japan
    Biotechnol Bioeng 90:509-15. 2005
    ..A reporter plasmid was constructed by inserting the beta-galactosidase gene (lacZ) under the control of the rpoS promoter, and then used to transform E. coli cells...
  54. pmc Escherichia coli starvation diets: essential nutrients weigh in distinctly
    Celeste N Peterson
    Department of Molecular Biology, Princeton University, NJ 08544, USA
    J Bacteriol 187:7549-53. 2005
  55. pmc A two-component phosphotransfer network involving ArcB, ArcA, and RssB coordinates synthesis and proteolysis of sigmaS (RpoS) in E. coli
    Franziska Mika
    Institut für Biologie Mikrobiologie, Freie Universitat Berlin, Germany
    Genes Dev 19:2770-81. 2005
    The general stress sigma factor sigma(S) (RpoS) in Escherichia coli is controlled at the levels of transcription, translation, and proteolysis...
  56. pmc Limited role for the DsrA and RprA regulatory RNAs in rpoS regulation in Salmonella enterica
    Amy M Jones
    Department of Microbiology, Immunology and Cell Biology, West Virginia University Health Sciences Center, Morgantown, WV 26506, USA
    J Bacteriol 188:5077-88. 2006
    b>RpoS, the sigma factor of enteric bacteria that responds to stress and stationary phase, is subject to complex regulation acting at multiple levels, including transcription, translation, and proteolysis...
  57. pmc General stress response signalling: unwrapping transcription complexes by DNA relaxation via the sigma38 C-terminal domain
    Yi Xin Huo
    Department of Chemistry and Biochemistry and the Molecular Biology Institute, University of California, PO Box 951569, Los Angeles, CA 90095, USA
    Mol Microbiol 70:369-78. 2008
    ..The general pathways typically require the master stress regulator sigma38 (rpoS)...
  58. doi Involvement of sigmaS accumulation in repression of the flhDC operon in acidic phospholipid-deficient mutants of Escherichia coli
    Junji Uchiyama
    Department of Biochemistry and Molecular Biology, Graduate School of Science and Engineering, Saitama University, 255 Shimo ohkubo, Saitama, Saitama 338 8570, Japan
    Microbiology 156:1650-60. 2010
    ..cells exhibited a sharp increase in sigma(S) levels that can only be partially attributed to the slight increase in rpoS transcription; the largest part of the effect is due to a post-transcriptional accumulation of sigma(S)...
  59. pmc Role of rpoS in the development of cell envelope resilience and pressure resistance in stationary-phase Escherichia coli
    Duangkamol Charoenwong
    Department of Food and Nutritional Sciences, The University of Reading, P O Box 226, Whiteknights, Reading RG6 6AP, United Kingdom
    Appl Environ Microbiol 77:5220-9. 2011
    This work investigated the role of rpoS in the development of increased cell envelope resilience and enhanced pressure resistance in stationary-phase cells of Escherichia coli...
  60. pmc Role of rpoS in Escherichia coli O157:H7 strain H32 biofilm development and survival
    Jessica R Sheldon
    Department of Biology, Lakehead University, Thunder Bay, Ontario, Canada
    Appl Environ Microbiol 78:8331-9. 2012
    The protein RpoS is responsible for mediating cell survival during the stationary phase by conferring cell resistance to various stressors and has been linked to biofilm formation...
  61. pmc Regulation of katF and katE in Escherichia coli K-12 by weak acids
    H E Schellhorn
    Department of Biology, McMaster University, Hamilton, Ontario, Canada
    J Bacteriol 174:4769-76. 1992
    ..transcriptional and translational lacZ fusions to the katE (structural gene for the HPII hydroperoxidase) and katF (putative sigma factor required for katE expression) genes of Escherichia coli were isolated, and the regulation of ..
  62. ncbi Are appR and katF the same Escherichia coli gene encoding a new sigma transcription initiation factor?
    E Touati
    Service de Biochimie des Proteines, CEN Saclay, Gif sur Yvette, France
    Res Microbiol 142:29-36. 1991
    The phenotype of Escherichia coli appR pleiotropic mutants has been compared with that of mutants in the katF gene, which lies in the same region and controls expression of catalase HPII (katE) and exonuclease III (xth)...
  63. ncbi The role of the 'gearbox' in the transcription of essential genes
    M Vicente
    Centro de Investigaciones Biologicas, Consejo Superior de Investigaciones Cientificas, Madrid, Spain
    Mol Microbiol 5:2085-91. 1991
    ..This suggests the existence of specific sigma factors for its transcription. RpoS (KatF) is a likely candidate for being one of these sigma factors...
  64. ncbi Cloning and physical characterization of katE and katF required for catalase HPII expression in Escherichia coli
    M R Mulvey
    Department of Microbiology, University of Manitoba, Winnipeg, Canada
    Gene 73:337-45. 1988
    Two genes, katE and katF, affecting the synthesis of catalase HPII in Escherichia coli, have been cloned...
  65. pmc Control of sensitivity to inactivation by H2O2 and broad-spectrum near-UV radiation by the Escherichia coli katF locus
    L J Sammartano
    J Bacteriol 168:13-21. 1986
    Mutations in the Escherichia coli katF gene (hydroperoxidase II) result in sensitivity to inactivation by H2O2 and broad-spectrum near-UV (NUV; 300 to 400 nm) radiation...
  66. ncbi The cellular concentration of the sigma S subunit of RNA polymerase in Escherichia coli is controlled at the levels of transcription, translation, and protein stability
    R Lange
    Department of Biology, University of Konstanz, Germany
    Genes Dev 8:1600-12. 1994
    The second vegetative sigma factor sigma S (encoded by the rpoS gene) is the master regulator in a complex regulatory network that governs the expression of many stationary phase-induced and osmotically regulated genes in Escherichia coli...
  67. ncbi The nlpD gene is located in an operon with rpoS on the Escherichia coli chromosome and encodes a novel lipoprotein with a potential function in cell wall formation
    R Lange
    Department of Biology, University of Konstanz, Germany
    Mol Microbiol 13:733-43. 1994
    b>rpoS is the structural gene for sigma s, which is a second vegetative sigma subunit of RNA polymerase in Escherichia coli and is involved in the expression of many stationary phase-induced genes...
  68. ncbi Structure of the 5' upstream region and the regulation of the rpoS gene of Escherichia coli
    Y Takayanagi
    Institute of Molecular and Cellular Biosciences, University of Tokyo, Japan
    Mol Gen Genet 243:525-31. 1994
    The nucleotide sequence of the 5' upstream region of the Escherichia coli rpoS gene was determined and analyzed...
  69. pmc Regulation of RNA polymerase sigma subunit synthesis in Escherichia coli: intracellular levels of four species of sigma subunit under various growth conditions
    M Jishage
    Department of Molecular Genetics, National Institute of Genetics, Mishima, Japan
    J Bacteriol 178:5447-51. 1996
    ..levels of two principal sigma subunits, sigma 70 (sigma D, the rpoD gene product) and sigma 38 (sigma S, the rpoS gene product), and of two minor sigma subunits, sigma 54 (sigma N, the rpoN gene product) and sigma 28 (sigma F, ..
  70. ncbi The sigma S level in starving Escherichia coli cells increases solely as a result of its increased stability, despite decreased synthesis
    H I Zgurskaya
    Department of Microbiology and Immunology, School of Medicine, Stanford University, California 94305, USA
    Mol Microbiol 24:643-51. 1997
    ..These increases occur despite reduced transcription of the sigma S-encoding gene, rpoS, and translation of rpoS mRNA, and result solely from a large increase in the stability of the sigma protein...
  71. pmc The LysR homolog LrhA promotes RpoS degradation by modulating activity of the response regulator sprE
    K E Gibson
    Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544, USA
    J Bacteriol 181:563-71. 1999
    ..We found that LrhA, a LysR homolog, functions as a regulatory component in the RpoS-dependent growth phase repression of ompF...
  72. pmc rpoS function is essential for bgl silencing caused by C-terminally truncated H-NS in Escherichia coli
    T Ohta
    Laboratory of Molecular Microbiology, School of Agriculture, Nagoya University, Chikusa ku, Nagoya 464 8601, Japan
    J Bacteriol 181:6278-83. 1999
    ..Genetic analysis revealed that the two insertions are within the rpoS gene and in front of the lrhA gene, respectively...
  73. pmc Global adaptations resulting from high population densities in Escherichia coli cultures
    X Liu
    Department of Microbiology, University of Sydney, Sydney, New South Wales, 2006, Australia
    J Bacteriol 182:4158-64. 2000
    ..The 10-fold-elevated level of trehalose at higher densities pointed to the increased role of the RpoS sigma factor, which controls trehalose synthesis genes as well as the general stress response...
  74. pmc Differential spectrum of mutations that activate the Escherichia coli bgl operon in an rpoS genetic background
    Sudha Moorthy
    Department of Molecular Reproduction, Development, and Genetics, Indian Institute of Science, Bangalore 560012, India
    J Bacteriol 184:4033-8. 2002
    ..The rpoS-encoded sigmaS, the stationary-phase sigma factor, is involved in both physiological as well as genetic changes ..
  75. ncbi Stationary phase gene regulation: what makes an Escherichia coli promoter sigmaS-selective?
    Regine Hengge-Aronis
    Institute of Biology, Microbiology, Freie Universitat Berlin, Königin Luise Strasse12 16, Berlin, Germany
    Curr Opin Microbiol 5:591-5. 2002
    ..These results throw novel light on the details of transcription initiation, as well as on the co-evolution of sigma factors and their cognate promoter sequences...
  76. pmc Sequential recognition of two distinct sites in sigma(S) by the proteolytic targeting factor RssB and ClpX
    Andrea Stüdemann
    Institut für Biologie Mikrobiologie, Freie Universitat Berlin, D 14195 Berlin, Germany
    EMBO J 22:4111-20. 2003
    sigma(S) (RpoS), the master regulator of the general stress response in Escherichia coli, is a model system for regulated proteolysis in bacteria...
  77. pmc A role for mechanosensitive channels in survival of stationary phase: regulation of channel expression by RpoS
    Neil R Stokes
    Department of Molecular and Cell Biology, Institute of Medical Sciences, University of Aberdeen, Aberdeen AB25 2ZD, Scotland, United Kingdom
    Proc Natl Acad Sci U S A 100:15959-64. 2003
    ..Reduced levels of MS proteins and reduced expression of mscL- and mscS-LacZ fusions in an rpoS mutant strain suggested that the RNA polymerase holoenzyme containing sigmaS is responsible, at least in part, for ..
  78. ncbi The multifactorial influences of RpoS, Mlc and cAMP on ptsG expression under glucose-limited and anaerobic conditions
    Shona Seeto
    School of Molecular and Microbial Biosciences, G08, University of Sydney, Sydney, NSW 2006, Australia
    Res Microbiol 155:211-5. 2004
    ..b>RpoS contributed to repression of ptsG in slow-growing bacteria but not under glucose-excess conditions...
  79. ncbi Controlled induction of the RpoS regulon in Escherichia coli, using an RpoS-expressing plasmid
    Guozhu Chen
    Department of Biology, McMaster University, 1280 Main Street West, Hamilton, ON L8S 4K1, Canada
    Can J Microbiol 49:733-40. 2003
    b>RpoS, an alternative sigma factor produced by many gram-negative bacteria, primarily controls genes that are expressed in stationary phase in response to nutrient deprivation...
  80. ncbi A differential effect of sigmaS on the expression of the PHO regulon genes of Escherichia coli
    Natalia Pasternak Taschner
    Departamento de Microbiologia, Instituto de Ciencias Biomedicas, Universidade de Sao Paulo, Av Professor Lineu Prestes, 1374, São Paulo SP CEP 05508 900, Brazil
    Microbiology 150:2985-92. 2004
    ..A mutation in the gene that encodes sigma(S), rpoS, significantly increases the level of alkaline phosphatase activity and the overproduction of sigma(S) inhibits it...
  81. pmc The TAO-Gen algorithm for identifying gene interaction networks with application to SOS repair in E. coli
    Takeharu Yamanaka
    Laboratory of Computational Biology and Risk Analysis, National Institute of Environmental Health Sciences, National Institutes of Health DHHS, 111 Alexander Drive, Research Triangle Park, NC 27709, USA
    Environ Health Perspect 112:1614-21. 2004
    ..The method is used to evaluate interactions between genes in the SOS signaling pathway in Escherichia coli using gene expression data where each gene in the network is over-expressed using plasmids inserts...
  82. ncbi ppGpp: a global regulator in Escherichia coli
    Lisa U Magnusson
    Department of Cell and Molecular Biology Microbiology, Goteborg University, Box 462, 405 30 Goteborg, Sweden
    Trends Microbiol 13:236-42. 2005
    ..In addition, new insights into the mechanism by which ppGpp affects gene expression have been achieved owing to in vitro studies of ppGpp function, complemented by structural studies of the ppGpp-RNA polymerase complex...
  83. ncbi The RpoS-mediated regulation of isocitrate dehydrogenase gene expression in Escherichia coli
    Ii Lae Jung
    Department of Radiation Biology, Environmental Radiation Research Group, Korea Atomic Energy Research Institute, P O Box 105, Yusong, Taejon 305 600, Korea
    Curr Microbiol 52:21-6. 2006
    ..Genetic inactivation of an rpoS gene, whose product is an alternative sigma factor, induced the icd gene expression approximately 4...
  84. ncbi Poly(A)-polymerase I links transcription with mRNA degradation via sigmaS proteolysis
    Jorge M Santos
    Instituto de Tecnologia Química e Biológica ITQB, Universidade Nova de Lisboa, Apt 127, 2781 901 Oeiras, Portugal
    Mol Microbiol 60:177-88. 2006
    ..Transcription of the Escherichia coli bolA gene is mediated by the RpoS/sigmaS transcription factor in response to environmental signals...
  85. ncbi [Stationary phase in Escherichia coli]
    Jesús Ramírez Santos
    Departamento de Biologia Molecular y Biotecnologia, Instituto de Investigaciones Biomedicas, Universidad Nacional Autonoma de Mexico
    Rev Latinoam Microbiol 47:92-101. 2005
    ..survival become transcribed in a manner dependent on the stationary phase-specific subunit of RNA polymerase (RpoS or sigma(s))...
  86. doi Nucleoid-associated proteins and bacterial physiology
    Charles J Dorman
    Department of Microbiology, School of Genetics and Microbiology, Trinity College, Dublin 2, Ireland
    Adv Appl Microbiol 67:47-64. 2009
    ....
  87. pmc The response regulator SprE (RssB) modulates polyadenylation and mRNA stability in Escherichia coli
    Valerie J Carabetta
    Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA
    J Bacteriol 191:6812-21. 2009
    In Escherichia coli, the adaptor protein SprE (RssB) controls the stability of the alternate sigma factor RpoS (sigma(38) and sigma(S))...
  88. pmc Transcription analysis of central metabolism genes in Escherichia coli. Possible roles of sigma38 in their expression, as a response to carbon limitation
    Leticia Olvera
    Departamento de Ingeniería Celular y Biocatálisis, Instituto de Biotecnologia, Universidad Nacional Autonoma de Mexico, Cuernavaca Morelos, Mexico
    PLoS ONE 4:e7466. 2009
    ..The products of the latter genes are involved in the production of AcetylCoA. Inactivation of rpoS that codes for the RNA polymerase sigma(38) subunit, reduces further (50%) growth of PB11, indicating that sigma(38)..
  89. ncbi Promoter selectivity control of Escherichia coli RNA polymerase by ionic strength: differential recognition of osmoregulated promoters by E sigma D and E sigma S holoenzymes
    Q Ding
    Department of Molecular Genetics, National Institute of Genetics, Shizuoka, Japan
    Mol Microbiol 16:649-56. 1995
    ....
  90. pmc Induction of the Escherichia coli aidB gene under oxygen-limiting conditions requires a functional rpoS (katF) gene
    M R Volkert
    Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, Worcester 01655
    J Bacteriol 176:7638-45. 1994
    ..Genetic and phenotypic analysis of the abrD1 mutation demonstrates that it is an allele of rpoS. Thus, aidB is a member of the family of genes that are transcribed by a sigma S-directed RNA polymerase holoenzyme...
  91. pmc Control of cell division in Escherichia coli: regulation of transcription of ftsQA involves both rpoS and SdiA-mediated autoinduction
    D M Sitnikov
    Center for Macromolecular Design, Texas A and M University, College Station 77843 2128, USA
    Proc Natl Acad Sci U S A 93:336-41. 1996
    ..coli, are regulated by promoters P1 and P2, located upstream of the ftsQ gene. The P1 promoter is rpoS-stimulated and the second, P2, is regulated by a member of the LuxR subfamily of transcriptional activators, SdiA, ..
  92. pmc The OxyS regulatory RNA represses rpoS translation and binds the Hfq (HF-I) protein
    A Zhang
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
    EMBO J 17:6061-8. 1998
    ..Among the OxyS targets is the rpoS-encoded sigma(s) subunit of RNA polymerase...
  93. pmc Positioning of sigma(S), the stationary phase sigma factor, in Escherichia coli RNA polymerase-promoter open complexes
    F Colland
    Institut Pasteur, Unité de Physico Chimie des Macromolécules Biologiques URA 1773 du CNRS, 75724 Paris Cedex 15, France
    EMBO J 18:4049-59. 1999
    ..2 reacts only weakly with the -35 promoter element. This provides a structural basis for the minor role of the -35 sequence in sigma(S)-dependent promoter recognition...
  94. ncbi Homocysteine thiolactone is a positive effector of sigma(S) levels in Escherichia coli
    H Goodrich-Blair
    Department of Bacteriology, University of Wisconsin Madison, Madison, WI, USA
    FEMS Microbiol Lett 185:117-21. 2000
    ....
  95. pmc Role of CspC and CspE in regulation of expression of RpoS and UspA, the stress response proteins in Escherichia coli
    S Phadtare
    Department of Biochemistry, Robert Wood Johnson Medical School, Piscataway, New Jersey 08854, USA
    J Bacteriol 183:1205-14. 2001
    ..b>RpoS is a regulatory element for osmY and dps...
  96. ncbi The cellular level of the recognition factor RssB is rate-limiting for sigmaS proteolysis: implications for RssB regulation and signal transduction in sigmaS turnover in Escherichia coli
    Mihaela Pruteanu
    Institut für Biologie Mikrobiologie, Freie Universtät Berlin, Germany
    Mol Microbiol 45:1701-13. 2002
    ..e. phosphorylated RssB...
  97. pmc The growth advantage in stationary-phase phenotype conferred by rpoS mutations is dependent on the pH and nutrient environment
    Michael J Farrell
    Molecular and Computation Biology Program, Department of Biological Sciences, University of Southern California, Los Angeles, California 90089 1340, USA
    J Bacteriol 185:7044-52. 2003
    ..A common early adaptation to this culture environment is a mutant rpoS allele, such as rpoS819, that results in attenuated RpoS activity...
  98. ncbi The DNA binding protein H-NS binds to and alters the stability of RNA in vitro and in vivo
    Cristin C Brescia
    Department of Microbiology and Immunology, Medical College of Ohio, 3055 Arlington Avenue, Toledo, OH 43614 5806, USA
    J Mol Biol 339:505-14. 2004
    ..One such gene, rpoS, specifies a sigma factor, RpoS...
  99. ncbi Identification of mutator genes and mutational pathways in Escherichia coli using a multicopy cloning approach
    Hanjing Yang
    Department of Microbiology, Immunology and Molecular Genetics, and the Molecular Biology Institute, 1602 Molecular Sciences Building, 405 Hilgard Avenue, University of California, Los Angeles, CA 90095, USA
    Mol Microbiol 53:283-95. 2004
    ..effects under overexpression conditions: error-prone polymerase IV (DinB), DNA methylase (Dam) and sigma S factor (RpoS)...
  100. pmc RpoS proteolysis is regulated by a mechanism that does not require the SprE (RssB) response regulator phosphorylation site
    Celeste N Peterson
    Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA
    J Bacteriol 186:7403-10. 2004
    In Escherichia coli the response regulator SprE (RssB) facilitates degradation of the sigma factor RpoS by delivering it to the ClpXP protease...
  101. pmc DNA looping-mediated repression by histone-like protein H-NS: specific requirement of Esigma70 as a cofactor for looping
    Minsang Shin
    Genome Research Center for Enteropathogenic Bacteria and Research Institute of Vibrio Infection, Department of Microbiology, Chonnam National University Medical School, Kwangju 501 746, South Korea
    Genes Dev 19:2388-98. 2005
    ..Expression of this class of genes by Esigma38 in the stationary phase is not due to its promoter specificity but to the architecture of the promoter . Esigma38 complex...

Research Grants1

  1. Molecular mechanisms of X. nematophila-nematode interaction
    Heidi Goodrich Blair; Fiscal Year: 2007
    ..nematophila during colonization, further define a colonization-specific regulon, and expand our knowledge of the role of a ubiquitous transcription factor in the natural ecology of a bacterium. [unreadable] [unreadable]..