rplK

Summary

Gene Symbol: rplK
Description: 50S ribosomal subunit protein L11
Alias: ECK3974, JW3946, relC
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. ncbi A detailed view of a ribosomal active site: the structure of the L11-RNA complex
    B T Wimberly
    Department of Biochemistry, University of Utah School of Medicine, Salt Lake City 84132, USA
    Cell 97:491-502. 1999
  2. ncbi The binding site for ribosomal protein complex L8 within 23 s ribosomal RNA of Escherichia coli
    A A Beauclerk
    J Biol Chem 259:6559-63. 1984
  3. pmc Cooperative assembly of proteins in the ribosomal GTPase centre demonstrated by their interactions with mutant 23S rRNAs
    G Rosendahl
    Department of Molecular Biology, Odense University, Denmark
    Nucleic Acids Res 23:2396-403. 1995
  4. ncbi Ribosomal proteins L11 and L10.(L12)4 and the antibiotic thiostrepton interact with overlapping regions of the 23 S rRNA backbone in the ribosomal GTPase centre
    G Rosendahl
    Department of Molecular Biology, Odense University, Denmark
    J Mol Biol 234:1013-20. 1993
  5. ncbi Localization of L11 protein on the ribosome and elucidation of its involvement in EF-G-dependent translocation
    R K Agrawal
    Wadsworth Center, Empire State Plaza, Albany, NY 12201 0509, USA
    J Mol Biol 311:777-87. 2001
  6. ncbi Characterization of the binding sites of protein L11 and the L10.(L12)4 pentameric complex in the GTPase domain of 23 S ribosomal RNA from Escherichia coli
    J Egebjerg
    Biostructural Chemistry, Chemistry Institute, Aarhus University, Denmark
    J Mol Biol 213:275-88. 1990
  7. ncbi Incorporation of aminoacyl-tRNA into the ribosome as seen by cryo-electron microscopy
    Mikel Valle
    Howard Hughes Medical Institute, Health Research, Inc at the Wadsworth Center, Empire State Plaza, Albany, New York 12201 0509, USA
    Nat Struct Biol 10:899-906. 2003
  8. ncbi Interaction of thiostrepton and elongation factor-G with the ribosomal protein L11-binding domain
    William S Bowen
    Division of Biological Sciences, The University of Montana, Missoula, Montana 59812, USA
    J Biol Chem 280:2934-43. 2005
  9. ncbi Interaction of the G' domain of elongation factor G and the C-terminal domain of ribosomal protein L7/L12 during translocation as revealed by cryo-EM
    Partha P Datta
    Division of Molecular Medicine, Wadsworth Center, New York State Department of Health, Empire State Plaza, P O Box 509, Albany, New York 12201, USA
    Mol Cell 20:723-31. 2005
  10. pmc Recognition of aminoacyl-tRNA: a common molecular mechanism revealed by cryo-EM
    Wen Li
    Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032, USA
    EMBO J 27:3322-31. 2008

Scientific Experts

  • R Todorova
  • RAJENDRA AGRAWAL
  • L B Blyn
  • D Shcherbakov
  • Natalya Van Dyke
  • Emanuel J Murgola
  • Lamine Bouakaz
  • Wen Li
  • Mans Ehrenberg
  • Joachim Frank
  • James R Iben
  • Hasan Demirci
  • Suparna Sanyal
  • Rose Marie Jenvert
  • Alberto García-Marcos
  • Yuhong Wang
  • David E Draper
  • Hanae Sato
  • Corina Maeder
  • William S Bowen
  • Partha P Datta
  • Mikel Valle
  • Thomas M Wendrich
  • X Yang
  • F Mueller
  • Rachel Green
  • Albert E Dahlberg
  • Julie L Brunelle
  • Xabier Agirrezabala
  • B T Wimberly
  • Gerwald Jogl
  • Steven T Gregory
  • Rodrigo F Ortiz-Meoz
  • Jianlin Lei
  • Miguel Remacha
  • Angel R Ortiz
  • Esther Guarinos
  • Juan P G Ballesta
  • Yale E Goldman
  • Antonio Morreale
  • Stanislas V Kirillov
  • Barry S Cooperman
  • Elisa Briones
  • Dongli Pan
  • Haiou Qin
  • Graham T Dempsey
  • Rama D Kudaravalli
  • Lovisa Holmberg Schiavone
  • Koichi Ito
  • G Rosendahl
  • Yoshikazu Nakamura
  • Graeme L Conn
  • Elli Bouakaz
  • Walter E Hill
  • J Stephen Lodmell
  • Li Qi
  • Manjuli R Sharma
  • Poul Nissen
  • Stephen C Harvey
  • Scott M Stagg
  • Rikke C Nielsen
  • Jayati Sengupta
  • Andrey Zavialov
  • Knud H Nierhaus
  • Gregor Blaha
  • Mohamed A Marahiel
  • Daniel N Wilson
  • Wenbing Xu
  • E E Ishiguro
  • M van Heel
  • E J Murgola
  • R Matadeen
  • I Sommer
  • M Stoldt
  • M Görlach
  • P Baranov
  • R Brimacombe
  • J Wöhnert
  • J P McCutcheon
  • V Ramakrishnan
  • R Guymon
  • S W White
  • S Douthwaite
  • A Vanet
  • A L Arkov
  • W Xu
  • J A Plumbridge
  • J H Alix
  • M F Guerin
  • W L Downing

Detail Information

Publications67

  1. ncbi A detailed view of a ribosomal active site: the structure of the L11-RNA complex
    B T Wimberly
    Department of Biochemistry, University of Utah School of Medicine, Salt Lake City 84132, USA
    Cell 97:491-502. 1999
    ..These antibiotics are proposed to bind in this cleft, locking the putative switch and interfering with the function of elongation factors...
  2. ncbi The binding site for ribosomal protein complex L8 within 23 s ribosomal RNA of Escherichia coli
    A A Beauclerk
    J Biol Chem 259:6559-63. 1984
    ..Previously, protein L11 had been shown to protect residues 1052-1112 of 23 S rRNA. It is concluded that the binding sites for the L8 protein complex and for protein L11 are immediately adjacent within 23 S rRNA of E. coli...
  3. pmc Cooperative assembly of proteins in the ribosomal GTPase centre demonstrated by their interactions with mutant 23S rRNAs
    G Rosendahl
    Department of Molecular Biology, Odense University, Denmark
    Nucleic Acids Res 23:2396-403. 1995
    ..Surprisingly, none of the mutations prevents incorporation of L11 into ribosomes in vivo, indicating that other, as yet unidentified, factors are involved in the cooperative assembly process...
  4. ncbi Ribosomal proteins L11 and L10.(L12)4 and the antibiotic thiostrepton interact with overlapping regions of the 23 S rRNA backbone in the ribosomal GTPase centre
    G Rosendahl
    Department of Molecular Biology, Odense University, Denmark
    J Mol Biol 234:1013-20. 1993
    ..L12)4 and other proteins within the ribosome. The antibiotics thiostrepton and micrococcin inhibit the catalytic functions of this region by slotting in between the accessible loops and interacting with nucleotides there...
  5. ncbi Localization of L11 protein on the ribosome and elucidation of its involvement in EF-G-dependent translocation
    R K Agrawal
    Wadsworth Center, Empire State Plaza, Albany, NY 12201 0509, USA
    J Mol Biol 311:777-87. 2001
    ..The results provide a new insight into the mechanism of EF-G-dependent translocation...
  6. ncbi Characterization of the binding sites of protein L11 and the L10.(L12)4 pentameric complex in the GTPase domain of 23 S ribosomal RNA from Escherichia coli
    J Egebjerg
    Biostructural Chemistry, Chemistry Institute, Aarhus University, Denmark
    J Mol Biol 213:275-88. 1990
    ..It also provides a structural rationale for the mutually co-operative binding of protein L11 with the antibiotics thiostrepton and micrococcin, and with the L10.(L12)4 protein complex...
  7. ncbi Incorporation of aminoacyl-tRNA into the ribosome as seen by cryo-electron microscopy
    Mikel Valle
    Howard Hughes Medical Institute, Health Research, Inc at the Wadsworth Center, Empire State Plaza, Albany, New York 12201 0509, USA
    Nat Struct Biol 10:899-906. 2003
    ..From these new findings we propose a mechanism that can explain the sequence of events during the decoding of mRNA on the ribosome...
  8. ncbi Interaction of thiostrepton and elongation factor-G with the ribosomal protein L11-binding domain
    William S Bowen
    Division of Biological Sciences, The University of Montana, Missoula, Montana 59812, USA
    J Biol Chem 280:2934-43. 2005
    ..The implications for recent models of thiostrepton activity and factor interactions are discussed...
  9. ncbi Interaction of the G' domain of elongation factor G and the C-terminal domain of ribosomal protein L7/L12 during translocation as revealed by cryo-EM
    Partha P Datta
    Division of Molecular Medicine, Wadsworth Center, New York State Department of Health, Empire State Plaza, P O Box 509, Albany, New York 12201, USA
    Mol Cell 20:723-31. 2005
    ....
  10. pmc Recognition of aminoacyl-tRNA: a common molecular mechanism revealed by cryo-EM
    Wen Li
    Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032, USA
    EMBO J 27:3322-31. 2008
    ....
  11. ncbi The flexible N-terminal domain of ribosomal protein L11 from Escherichia coli is necessary for the activation of stringent factor
    Rose Marie Jenvert
    School of Life Sciences, Södertörns högskola, S 141 04 Huddinge, Sweden
    J Mol Biol 365:764-72. 2007
    ..This suggests that the N-terminal domain can activate stringent factor in trans. It is also shown that this activation is dependent on unacylated tRNA...
  12. pmc Characterization of promoter-cloning plasmids: analysis of operon structure in the rif region of Escherichia coli and isolation of an enhanced internal promoter mutant
    G An
    J Bacteriol 144:904-16. 1980
    ..Analysis of a variety of plasmids in which tet is fused to various promoters yielded the following results: (i) rplK and rplA, genes for ribosomal protein L11 and L1, respectively, were cotranscribed from a common promoter located ..
  13. ncbi Purification and primary structure determination of the N-terminal blocked protein, L11, from Escherichia coli ribosomes
    M J Dognin
    Eur J Biochem 112:131-51. 1980
    ..The protein contains 141 amino acid residues and has a molecular weight of 14874. Secondary structure predictions of the protein are given, and its sequence is compared with those of other E. coli ribosomal proteins...
  14. pmc Multiple-site trimethylation of ribosomal protein L11 by the PrmA methyltransferase
    Hasan Demirci
    Department of Molecular Biology, Cell Biology and Biochemistry, Brown University, Providence, RI 02912, USA
    Structure 16:1059-66. 2008
    ....
  15. pmc Transcriptional and post-transcriptional control of ribosomal protein and ribonucleic acid polymerase genes
    R Little
    J Bacteriol 147:25-35. 1981
    ..Furthermore, the observed reduction in the translation efficiency of beta subunit messenger RNA may be related to an inhibitory effect caused by accumulation of RNA polymerase assembly intermediates...
  16. doi Specific interactions of the L10(L12)4 ribosomal protein complex with mRNA, rRNA, and L11
    James R Iben
    Department of Biophysics, Johns Hopkins University, Baltimore, Maryland 21218, USA
    Biochemistry 47:2721-31. 2008
    ....
  17. ncbi In vivo synthesis of a polycistronic messenger RNA for the ribosomal proteins L11, L1, L10 and L7/12 in Escherichia coli
    R Bruckner
    Mol Gen Genet 183:277-82. 1981
    ..Smaller distinct mRNA species could also be detected by this technique. They originate from intercistronic transcription termination and re-initiation as well as from processing of the larger polycistronic mRNA...
  18. pmc In vivo assembling of bacterial ribosomal protein L11 into yeast ribosomes makes the particles sensitive to the prokaryotic specific antibiotic thiostrepton
    Alberto García-Marcos
    Centro de Biologia Molecular Severo Ochoa, Universidad Autónoma de Madrid and Consejo Superior de Investigaciones Científicas, Cantoblanco, Madrid 28049, Spain
    Nucleic Acids Res 35:7109-17. 2007
    ..Molecular dynamic simulations using modelled complexes support the correct assembly of bacterial L11 into the yeast ribosome and confirm its direct implication of its CTD in the binding of thiostrepton to ribosomes...
  19. ncbi Single-molecule structural dynamics of EF-G--ribosome interaction during translocation
    Yuhong Wang
    Pennsylvania Muscle Institute, School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104 6083, USA
    Biochemistry 46:10767-75. 2007
    ..6 state results from the conformational lability of unlocked ribosomes formed during translocation. An idling state, possibly pertinent to regulation of protein synthesis, is detected in some ribosomes in the poly(Phe) system...
  20. ncbi Mutations at three sites in the Escherichia coli 23S ribosomal RNA binding region for protein L11 cause UGA-specific suppression and conditional lethality
    E J Murgola
    Department of Molecular Genetics, University of Texas M D Anderson Cancer Center, Houston 77030, USA
    Nucleic Acids Symp Ser . 1995
    ....
  21. ncbi The role of ribosomal protein L11 in class I release factor-mediated translation termination and translational accuracy
    Lamine Bouakaz
    Institute of Cell and Molecular Biology, Uppsala University, Sweden
    J Biol Chem 281:4548-56. 2006
    ....
  22. pmc The RNA-binding domain of ribosomal protein L11 recognizes an rRNA tertiary structure stabilized by both thiostrepton and magnesium ion
    L B Blyn
    Ibis Therapeutics, A Division of Isis Pharmaceuticals, 2292 Faraday Avenue, Carlsbad, CA 92008, USA
    Nucleic Acids Res 28:1778-84. 2000
    ..Experiments using these assays with both an Escherichia coli rRNA fragment and a thermostable variant of that RNA show that Mg(2+), L11 and thiostrepton all induce the RNA to fold to an essentially identical tertiary structure...
  23. ncbi The 3D arrangement of the 23 S and 5 S rRNA in the Escherichia coli 50 S ribosomal subunit based on a cryo-electron microscopic reconstruction at 7.5 A resolution
    F Mueller
    Max Planck Institut fur Molekulare Genetik, Ihnestrasse 73, Berlin, 14195, Germany
    J Mol Biol 298:35-59. 2000
    ....
  24. pmc Stability of the 'L12 stalk' in ribosomes from mesophilic and (hyper)thermophilic Archaea and Bacteria
    D Shcherbakov
    Biocenter, Division of Medical Biochemistry, Innsbruck Medical University, Fritz Pregl Strasse 3, 6020, Innsbruck, Austria
    Nucleic Acids Res 34:5800-14. 2006
    ..A cooperative effect of binding to 23S rRNA of protein L11 and the L10/L12(4) complex from mesophilic and thermophilic Archaea was shown to be temperature-dependent...
  25. pmc Involvement of the N terminus of ribosomal protein L11 in regulation of the RelA protein of Escherichia coli
    X Yang
    Department of Biochemistry and Microbiology, University of Victoria, British Columbia, Canada
    J Bacteriol 183:6532-7. 2001
    Amino acid-deprived rplK (previously known as relC) mutants of Escherichia coli cannot activate (p)ppGpp synthetase I (RelA) and consequently exhibit relaxed phenotypes...
  26. ncbi Limitation of ribosomal protein L11 availability in vivo affects translation termination
    Natalya Van Dyke
    Department of Molecular Genetics, Box 11, The University of Texas M D Anderson Cancer Center, 1515 Holcombe Boulevard, Houston, TX 77030 4009, USA
    J Mol Biol 319:329-39. 2002
    ....
  27. ncbi Dissection of the mechanism for the stringent factor RelA
    Thomas M Wendrich
    Fachbereich Chemie Biochemie, Philipps Universitat Marburg, Hans Meerwein Strasse, D 35032, Marburg, Germany
    Mol Cell 10:779-88. 2002
    ....
  28. ncbi Site of functional interaction of release factor 1 with the ribosome
    Natalya Van Dyke
    Department of Molecular Genetics, The University of Texas M D Anderson Cancer Center, Box 11, 1515 Holcombe Boulevard, Houston 77030 4009, USA
    J Mol Biol 330:9-13. 2003
    ..Our results show for the first time that it is precisely the N-terminal part of ribosomal protein L11 that is required for the functional interaction of release factor 1 with the ribosome in the cell...
  29. ncbi Specific binding of ribosome recycling factor (RRF) with the Escherichia coli ribosomes by BIACORE
    Roumiana T Todorova
    Institute of Biophysics, Bulgarian Academy of Sciences, 1113 Sofia, Bulgaria
    Mol Biol Rep 30:113-9. 2003
    ..We can consider an electrostatic character of the interactions with most probable candidate 16S and 23S r RNA at the interface of 30S and 50S ribosomal subunits...
  30. pmc Optimization of a ribosomal structural domain by natural selection
    Corina Maeder
    Program in Molecular and Computational Biophysics and Department of Chemistry, Johns Hopkins University, Baltimore, Maryland 21218, USA
    Biochemistry 45:6635-43. 2006
    ....
  31. ncbi Ribosomal protein L11 mutations in two functional domains equally affect release factors 1 and 2 activity
    Hanae Sato
    Department of Basic Medical Sciences, Institute of Medical Science, University of Tokyo, 4 6 1 Shirokanedai, Minato ku, Tokyo 108 8639, Japan
    Mol Microbiol 60:108-20. 2006
    ..These L11 mutations were located on the surface of two domains of L11, and interpreted to affect the interaction between L11 and rRNA or the RFs thereby leading to the altered translation termination...
  32. pmc Sequence and transcriptional pattern of the essential Escherichia coli secE-nusG operon
    W L Downing
    Department of Biochemistry, University of British Columbia, Vancouver, Canada
    J Bacteriol 172:1621-7. 1990
    ..transcripts initiated at the PEG promoter and terminated at the Rho-independent terminator in the region of the rplK promoter...
  33. pmc Lambda transducing bacteriophage carrying deletions of the argCBH-rpoBC region of the Escherichia coli chromosome
    T Linn
    J Bacteriol 140:479-89. 1979
    ..We thus extend and confirm knowledge of the organization of this part of the chromosome. The new phages are useful tools for studying the genes for the bacterial transcription and translation machinery...
  34. ncbi A new technique for selection of sensitive and auxotrophic mutants of E. coli: isolation of a strain sensitive to an excess of one-carbon metabolites
    A Danchin
    Mol Gen Genet 150:293-9. 1977
    ..It carries two mutations, smg A1 (near metA and arg H), probably identical to relC, and smgB (between asn and ilv), probably part of the E. coli membrane ATPase.
  35. pmc Kasugamycin-dependent mutants of Escherichia coli
    E R Dabbs
    J Bacteriol 136:994-1001. 1978
    ..Three showed changes in protein S9. Each of two mutants had changes in two proteins, S18 and L11. Three of these mutants additionally had protein S18 occurring in a partly altered, partly unaltered form...
  36. pmc Nucleotide sequence of the ribosomal protein gene cluster adjacent to the gene for RNA polymerase subunit beta in Escherichia coli
    L E Post
    Proc Natl Acad Sci U S A 76:1697-701. 1979
    ..The sequence covers the genes for ribosomal proteins L11 (rplK), LI (rplA), L10 (rplJ), and L7/L12 ((rplL), and the 5' end of the gene for the beta subunit of RNA polymerase (..
  37. ncbi In vivo analysis of overlapping transcription units in the rplKAJLrpoBC ribosomal protein-RNA polymerase gene cluster of Escherichia coli
    K L Steward
    Department of Microbiology and Immunology, Faculty of Medicine, University of Western Ontario, London, Canada
    J Mol Biol 218:23-31. 1991
    ..The results show that transcription initiated at the upstream rplKp promoter (located just before rplK) does not terminate before the rplJp promoter (located upstream from rplJ), but instead reads through into the ..
  38. ncbi Recognition of the highly conserved GTPase center of 23 S ribosomal RNA by ribosomal protein L11 and the antibiotic thiostrepton
    P C Ryan
    Department of Chemistry, Johns Hopkins University, Baltimore, MD 21218
    J Mol Biol 221:1257-68. 1991
    ..The results are discussed in terms of a model in which conformational flexibility of the GTPase center RNA is functionally important during the ribosome elongation cycle...
  39. ncbi Escherichia coli RNA polymerase binding and initiation of transcription on fragments of lambda rifd 18 DNA containing promoters for lambda genes and for rrnB, tufB, rplC,A, rplJ,L, and rpoB,C genes
    W E Taylor
    Gene 6:331-65. 1979
    ..lambda and for Escherichia coli ribosomal RNA (rrnB), elongation factor Tu (tufB), ribosomal proteins L11 (rplK), L1 (rplA), L10 (rplJ), and L7/L12 (rplL), and RNA polymerase subunits beta (rpoB) and beta' (rpoC) were studied ..
  40. ncbi Transcription products from the rplKAJL-rpoBC gene cluster
    W L Downing
    Department of Biochemistry, University of British Columbia, Vancouver, Canada
    J Mol Biol 194:609-20. 1987
    ..These results indicate that the two major promoters, PL11 and PL10, are both utilized to drive the interrelated transcriptional expression of this ribosomal protein-RNA polymerase gene cluster...
  41. ncbi Structural properties of ribosomal protein L11 from Escherichia coli
    T Choli
    Max Planck Institut fur Molekulare Genetik, Berlin, FRG
    Biochem Int 19:1323-38. 1989
    ..It appears probable that the central region of L11, in which the protease cleavages occur, plays an important part in structural and functional aspects...
  42. ncbi Subunit association defects in Escherichia coli ribosome mutants lacking proteins S20 and L11
    F Gotz
    Max Planck Institut fur Molekulare Genetik, Abteilung Wittmann, Berlin Dahlem
    Eur J Biochem 183:19-24. 1989
    ....
  43. pmc Translational regulation of the L11 ribosomal protein operon of Escherichia coli: mutations that define the target site for repression by L1
    M S Thomas
    Nucleic Acids Res 15:3085-96. 1987
    ..These results are also consistent with the previously proposed model for the secondary structure of the L11 leader mRNA...
  44. ncbi Cloning of DNA of the rpoBC operon from the chromosome of Escherichia coli K12
    A Newman
    Mol Gen Genet 177:527-33. 1980
    ..As in lambdarifd 18 itself, this promoter is shared with rplJ but not with rplKA. The properties of the latter phage also show that the dominant rifampicin-resistance characteristic of lambdarifd 18 results from more than one mutation...
  45. pmc Insertions of transposon Tn5 into ribosomal protein PNA polymerase operons
    I Hui
    J Bacteriol 152:1022-32. 1982
    ..The results confirmed the organization of these genes into two transcription units as follows: PL11, rplK (L11), rplA (L1), PL10, rplJ (L10), rplL (L12), rpoB (beta). . ...
  46. pmc Relative activities of the transcriptional regulatory sites in the rplKAJLrpoBC gene cluster of Escherichia coli
    G Ralling
    J Bacteriol 158:279-85. 1984
    ..We find that the promoters preceding rplK (rplKp) and rplJ (rplJp) are indeed the major promoters of this gene cluster...
  47. ncbi Ribosomal protein methylation in Escherichia coli: the gene prmA, encoding the ribosomal protein L11 methyltransferase, is dispensable
    A Vanet
    Institut de Biologie Physico Chimique, URA1139 CNRS, Paris, France
    Mol Microbiol 14:947-58. 1994
    ..However, a strain carrying an in vitro-constructed null mutation of the prmA gene, transferred to the E. coli chromosome by homologous recombination, was perfectly viable...