rnhB

Summary

Gene Symbol: rnhB
Description: ribonuclease HII, degrades RNA of DNA-RNA hybrids
Alias: ECK0182, JW0178
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. pmc Excision of misincorporated ribonucleotides in DNA by RNase H (type 2) and FEN-1 in cell-free extracts
    Bjorn Rydberg
    Life Sciences Division, Lawrence Berkeley National Laboratory, 1 Cyclotron Road, Berkeley, CA 94720, USA
    Proc Natl Acad Sci U S A 99:16654-9. 2002
  2. pmc Isolation and characterization of a second RNase H (RNase HII) of Escherichia coli K-12 encoded by the rnhB gene
    M Itaya
    Department of Molecular Biology, Mitsubishi Kasei Institute of Life Sciences, Tokyo, Japan
    Proc Natl Acad Sci U S A 87:8587-91. 1990
  3. pmc Sequence analysis of the Escherichia coli dnaE gene
    H G Tomasiewicz
    Department of Biochemistry, Biophysics and Genetics, University of Colorado Health Sciences Center, Denver 80262
    J Bacteriol 169:5735-44. 1987
  4. ncbi Identification of the genes encoding Mn2+-dependent RNase HII and Mg2+-dependent RNase HIII from Bacillus subtilis: classification of RNases H into three families
    N Ohtani
    Department of Material and Life Science, Graduate School of Engineering, Osaka University, Japan
    Biochemistry 38:605-18. 1999
  5. ncbi Characterization of ribonuclease HII from Escherichia coli overproduced in a soluble form
    N Ohtani
    Department of Material and Life Science, Graduate School of Engineering, Osaka University, Yamadaoka, Suita, Osaka 565 0871, Japan
    J Biochem 127:895-9. 2000
  6. pmc LuxArray, a high-density, genomewide transcription analysis of Escherichia coli using bioluminescent reporter strains
    T K Van Dyk
    Central Research and Development Department, DuPont Company, Wilmington, Delaware 19880 0173, USA
    J Bacteriol 183:5496-505. 2001
  7. ncbi The pH-dependence of the Escherichia coli RNase HII-catalysed reaction suggests that an active site carboxylate group participates directly in catalysis
    James A Bastock
    Centre for Chemical Biology, Department of Chemistry, University of Sheffield, Sheffield, S3 7HF, UK
    J Mol Biol 368:421-33. 2007
  8. doi Ribonuclease H: molecular diversities, substrate binding domains, and catalytic mechanism of the prokaryotic enzymes
    Takashi Tadokoro
    Department of Material and Life Science, Osaka University, Suita, Osaka, Japan
    FEBS J 276:1482-93. 2009

Scientific Experts

  • Tina K Van Dyk
  • Takashi Tadokoro
  • N Ohtani
  • James A Bastock
  • Bjorn Rydberg
  • Shigenori Kanaya
  • M Morikawa
  • S Kanaya
  • M Haruki
  • Michelle Webb
  • Jane A Grasby
  • John Game
  • M Itaya
  • A Muroya
  • R J Crouch
  • H G Tomasiewicz
  • C S McHenry

Detail Information

Publications8

  1. pmc Excision of misincorporated ribonucleotides in DNA by RNase H (type 2) and FEN-1 in cell-free extracts
    Bjorn Rydberg
    Life Sciences Division, Lawrence Berkeley National Laboratory, 1 Cyclotron Road, Berkeley, CA 94720, USA
    Proc Natl Acad Sci U S A 99:16654-9. 2002
    ..We knocked out the corresponding rnhb gene in E. coli and show that extracts from this strain lack the nicking activity...
  2. pmc Isolation and characterization of a second RNase H (RNase HII) of Escherichia coli K-12 encoded by the rnhB gene
    M Itaya
    Department of Molecular Biology, Mitsubishi Kasei Institute of Life Sciences, Tokyo, Japan
    Proc Natl Acad Sci U S A 87:8587-91. 1990
    ..The rnhB gene, which encodes RNase HII, was distinct from rnhA by its map position (4.5 min on E...
  3. pmc Sequence analysis of the Escherichia coli dnaE gene
    H G Tomasiewicz
    Department of Biochemistry, Biophysics and Genetics, University of Colorado Health Sciences Center, Denver 80262
    J Bacteriol 169:5735-44. 1987
    ..11) with a molecular weight of 23,228. In the accompanying paper (D.N. Crowell, W.S. Reznikoff, and C.R.H. Raetz, J. Bacteriol. 169:5727-5734, 1987), the sequence of the upstream region that contains lpxA and lpxB is reported...
  4. ncbi Identification of the genes encoding Mn2+-dependent RNase HII and Mg2+-dependent RNase HIII from Bacillus subtilis: classification of RNases H into three families
    N Ohtani
    Department of Material and Life Science, Graduate School of Engineering, Osaka University, Japan
    Biochemistry 38:605-18. 1999
    ..In contrast, the rnh (rnhB) gene product (RNase HII) showed a preference for Mn2+, as did E...
  5. ncbi Characterization of ribonuclease HII from Escherichia coli overproduced in a soluble form
    N Ohtani
    Department of Material and Life Science, Graduate School of Engineering, Osaka University, Yamadaoka, Suita, Osaka 565 0871, Japan
    J Biochem 127:895-9. 2000
    ....
  6. pmc LuxArray, a high-density, genomewide transcription analysis of Escherichia coli using bioluminescent reporter strains
    T K Van Dyk
    Central Research and Development Department, DuPont Company, Wilmington, Delaware 19880 0173, USA
    J Bacteriol 183:5496-505. 2001
    ..Thus, the promoters of ycgH, intG, rihC, and a putative operon consisting of lpxA, lpxB, rnhB, and dnaE were not generally DNA damage responsive and represent a more specific response to nalidixic acid...
  7. ncbi The pH-dependence of the Escherichia coli RNase HII-catalysed reaction suggests that an active site carboxylate group participates directly in catalysis
    James A Bastock
    Centre for Chemical Biology, Department of Chemistry, University of Sheffield, Sheffield, S3 7HF, UK
    J Mol Biol 368:421-33. 2007
    ....
  8. doi Ribonuclease H: molecular diversities, substrate binding domains, and catalytic mechanism of the prokaryotic enzymes
    Takashi Tadokoro
    Department of Material and Life Science, Osaka University, Suita, Osaka, Japan
    FEBS J 276:1482-93. 2009
    ..We review the results of these studies...