recR

Summary

Gene Symbol: recR
Description: gap repair protein
Alias: ECK0466, JW0461
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. ncbi Identification of the recR locus of Escherichia coli K-12 and analysis of its role in recombination and DNA repair
    A A Mahdi
    Department of Genetics, University of Nottingham, Queens Medical Centre, UK
    Mol Gen Genet 216:503-10. 1989
  2. ncbi Spontaneous and cisplatin-induced recombination in Escherichia coli
    Anetta Nowosielska
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, 364 Plantation Street, LRB823 Worcester, MA 01655, USA
    DNA Repair (Amst) 3:719-28. 2004
  3. ncbi RecFOR proteins load RecA protein onto gapped DNA to accelerate DNA strand exchange: a universal step of recombinational repair
    Katsumi Morimatsu
    Section of Microbiology, Center for Genetics and Development, University of California, Davis, 95616, USA
    Mol Cell 11:1337-47. 2003
  4. pmc Imaging and energetics of single SSB-ssDNA molecules reveal intramolecular condensation and insight into RecOR function
    Jason C Bell
    Graduate Group in Biochemistry and Molecular Biology, University of California, Davis, Davis, United States
    elife 4:e08646. 2015
  5. ncbi DNA strand exchange proteins: a biochemical and physical comparison
    P R Bianco
    Section of Microbiology and of Molecular and Cellular Biology, USC School of Medicine, 1441 Eastlake Ave, Los Angeles, CA 90033, USA
    Front Biosci 3:D570-603. 1998
  6. pmc Interactions of RecF protein with RecO, RecR, and single-stranded DNA binding proteins reveal roles for the RecF-RecO-RecR complex in DNA repair and recombination
    S P Hegde
    Department of Microbiology, University of Texas Health Center at Tyler 75710, USA
    Proc Natl Acad Sci U S A 93:14468-73. 1996
  7. pmc Distinguishing characteristics of hyperrecombinogenic RecA protein from Pseudomonas aeruginosa acting in Escherichia coli
    Dmitry M Baitin
    Division of Molecular and Radiation Biophysics, Petersburg Nuclear Physics Institute, Russian Academy of Sciences, Gatchina St Petersburg 188300, Russia
    J Bacteriol 188:5812-20. 2006
  8. ncbi RecO acts with RecF and RecR to protect and maintain replication forks blocked by UV-induced DNA damage in Escherichia coli
    Kin Hoe Chow
    Department of Biological Science, Mississippi State University, Mississippi State, Mississippi 39762, USA
    J Biol Chem 279:3492-6. 2004
  9. ncbi Requirement for RecFOR-mediated recombination in priA mutant
    Gianfranco Grompone
    Génétique Microbienne, Institut National de la Recherche Agronomique, Domaine de Vilvert 78350 Jouy en Josas, France
    Mol Microbiol 52:551-62. 2004
  10. pmc The recR locus of Escherichia coli K-12: molecular cloning, DNA sequencing and identification of the gene product
    A A Mahdi
    Department of Genetics, University of Nottingham, Queens Medical Centre, UK
    Nucleic Acids Res 17:6781-94. 1989

Scientific Experts

  • Xavier Veaute
  • J Courcelle
  • Hideo Ikeda
  • Kin Hoe Chow
  • Naofumi Handa
  • Anetta Nowosielska
  • Stephen C Kowalczykowski
  • Michael D Hobbs
  • Jason C Bell
  • Michael M Cox
  • Bénédicte Michel
  • Christian J Rudolph
  • Roxane Lestini
  • Dmitry M Baitin
  • Shelley L Lusetti
  • Ivana Ivancić-Bacće
  • Shingo Fujii
  • Krunoslav Brcic-Kostic
  • B L Webb
  • Erika Salaj-Smic
  • Robert W Maul
  • Ivana Ivancic-Bace
  • R B Inman
  • M G Marinus
  • M M Cox
  • J M Bork
  • Gianfranco Grompone
  • Katsumi Morimatsu
  • Liewei Xu
  • Enrique Viguera
  • Bian Liu
  • Kouya Shiraishi
  • Noriko Kantake
  • Savithri Rangarajan
  • G A Cromie
  • B Michel
  • Robert G Lloyd
  • Amy L Upton
  • Akiko Sakai
  • Irina V Bakhlanova
  • P R Bianco
  • Ignacija Vlasic
  • Gordana Cogelja-Cajo
  • Robert P Fuchs
  • H Steven Seifert
  • S C Kowalczykowski
  • Yury V Kil
  • Elizabeth A Stohl
  • Ross B Inman
  • Stephen A Smith
  • Bevin P Engelward
  • Asako Isogawa
  • Vladislav A Lanzov
  • Sindhu Chitteni-Pattu
  • Mark D Sutton
  • K Umezu
  • S Dusko Ehrlich
  • Zoran Zdraveski
  • Q Shan
  • John M Essigmann
  • Melissa A Calmann
  • A J Clark
  • Nicolas Sanchez
  • Davor Zahradka
  • Mirjana Petranovic
  • Karine Germain
  • Kenneth J Marians
  • Dusko S Ehrlich
  • Roger Woodgate
  • Murty V V M Madiraju
  • Katsuhiro Hanada
  • Yoichiro Iwakura
  • Tomohiko Sugiyama
  • Myron F Goodman
  • S P Hegde
  • D R Leach
  • M Penel-Colin
  • G D Recchia
  • S D Ehrlich
  • M C Whitby
  • D J Sherratt
  • R B Tracy
  • R G Lloyd
  • R D Kolodner
  • M A Atkinson
  • M V Madiraju
  • X H Li
  • M Rajagopalan
  • M H Qin
  • S D Lauder

Detail Information

Publications43

  1. ncbi Identification of the recR locus of Escherichia coli K-12 and analysis of its role in recombination and DNA repair
    A A Mahdi
    Department of Genetics, University of Nottingham, Queens Medical Centre, UK
    Mol Gen Genet 216:503-10. 1989
    A new recombination gene called recR has been identified and located near dnaZ at minute 11 on the current linkage map of Escherichia coli...
  2. ncbi Spontaneous and cisplatin-induced recombination in Escherichia coli
    Anetta Nowosielska
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, 364 Plantation Street, LRB823 Worcester, MA 01655, USA
    DNA Repair (Amst) 3:719-28. 2004
    ..Inactivation of the recF, recO, recR and recJ genes decreased cisplatin-induced, but not spontaneous, recombination...
  3. ncbi RecFOR proteins load RecA protein onto gapped DNA to accelerate DNA strand exchange: a universal step of recombinational repair
    Katsumi Morimatsu
    Section of Microbiology, Center for Genetics and Development, University of California, Davis, 95616, USA
    Mol Cell 11:1337-47. 2003
    Genetic evidence suggests that the RecF, RecO, and RecR (RecFOR) proteins participate in a common step of DNA recombination and repair, yet the biochemical event requiring collaboration of all three proteins is unknown...
  4. pmc Imaging and energetics of single SSB-ssDNA molecules reveal intramolecular condensation and insight into RecOR function
    Jason C Bell
    Graduate Group in Biochemistry and Molecular Biology, University of California, Davis, Davis, United States
    elife 4:e08646. 2015
    ....
  5. ncbi DNA strand exchange proteins: a biochemical and physical comparison
    P R Bianco
    Section of Microbiology and of Molecular and Cellular Biology, USC School of Medicine, 1441 Eastlake Ave, Los Angeles, CA 90033, USA
    Front Biosci 3:D570-603. 1998
    ..coli, UvsX protein from Bacteriophage T4, and RAD51 protein from Saccharomyces cerevisiae...
  6. pmc Interactions of RecF protein with RecO, RecR, and single-stranded DNA binding proteins reveal roles for the RecF-RecO-RecR complex in DNA repair and recombination
    S P Hegde
    Department of Microbiology, University of Texas Health Center at Tyler 75710, USA
    Proc Natl Acad Sci U S A 93:14468-73. 1996
    The products of the recF, recO, and recR genes are thought to interact and assist RecA in the utilization of single-stranded DNA precomplexed with single-stranded DNA binding protein (Ssb) during synapsis...
  7. pmc Distinguishing characteristics of hyperrecombinogenic RecA protein from Pseudomonas aeruginosa acting in Escherichia coli
    Dmitry M Baitin
    Division of Molecular and Radiation Biophysics, Petersburg Nuclear Physics Institute, Russian Academy of Sciences, Gatchina St Petersburg 188300, Russia
    J Bacteriol 188:5812-20. 2006
    ..Expression of RecA(Pa) also partially suppresses the effects of recF, recO, and recR mutations...
  8. ncbi RecO acts with RecF and RecR to protect and maintain replication forks blocked by UV-induced DNA damage in Escherichia coli
    Kin Hoe Chow
    Department of Biological Science, Mississippi State University, Mississippi State, Mississippi 39762, USA
    J Biol Chem 279:3492-6. 2004
    In Escherichia coli, recF and recR are required to stabilize and maintain replication forks arrested by UV-induced DNA damage...
  9. ncbi Requirement for RecFOR-mediated recombination in priA mutant
    Gianfranco Grompone
    Génétique Microbienne, Institut National de la Recherche Agronomique, Domaine de Vilvert 78350 Jouy en Josas, France
    Mol Microbiol 52:551-62. 2004
    ..Models for the formation of single strand DNA gaps consequently to a replication restart defect and for gap processing are proposed...
  10. pmc The recR locus of Escherichia coli K-12: molecular cloning, DNA sequencing and identification of the gene product
    A A Mahdi
    Department of Genetics, University of Nottingham, Queens Medical Centre, UK
    Nucleic Acids Res 17:6781-94. 1989
    The recR gene of Escherichia coli, which is associated with recBC-independent mechanisms of recombination and DNA repair, has been located between dnaZX and htpG on a 6...
  11. ncbi Illegitimate recombination mediated by double-strand break and end-joining in Escherichia coli
    Hideo Ikeda
    Institute of Molecular Genetics, Medinet Inc, Tamagawadai 2 2 8, Setagaya Ku, Tokyo 158 0096, Japan
    Adv Biophys 38:3-20. 2004
    ..the frequencies of spontaneous and UV-induced illegitimate recombination and that the RecJ, RecF, RecO, and RecR functions are required for this RecE-mediated illegitimate recombination...
  12. ncbi Illegitimate recombination mediated by double-strand break and end-joining in Escherichia coli
    Hideo Ikeda
    Institute of Molecular Genetics, Medinet Inc, Tamagawadai 2 2 8, Setagaya Ku, Tokyo 158 0096, Japan
    Adv Biophys 38:3-20. 2004
    ..the frequencies of spontaneous and UV-induced illegitimate recombination and that the RecJ, RecF, RecO, and RecR functions are required for this RecE-mediated illegitimate recombination...
  13. pmc UvrD helicase, unlike Rep helicase, dismantles RecA nucleoprotein filaments in Escherichia coli
    Xavier Veaute
    CEA, DSV, DRR, UMR217 CNRS CEA, Fontenay aux Roses, France
    EMBO J 24:180-9. 2005
    ..We reported earlier that mutations in recF, recO or recR genes suppress the lethality of uvrD rep, and proposed that an essential activity common to UvrD and Rep is either ..
  14. pmc Effects of recJ, recQ, and recFOR mutations on recombination in nuclease-deficient recB recD double mutants of Escherichia coli
    Ivana Ivancic-Bace
    Department of Molecular Biology, Ruder Boskovic Institute, Bijenicka 54, HR 10002 Zagreb, Croatia
    J Bacteriol 187:1350-6. 2005
    ..We propose that the helicase and RecA loading activities belong to the RecBCD recombination machinery, while the RecJ-mediated 5'-->3' exonuclease is an element of the RecF recombination machinery...
  15. pmc Roles of the Escherichia coli RecA protein and the global SOS response in effecting DNA polymerase selection in vivo
    Robert W Maul
    Department of Biochemistry, School of Medicine and Biomedical Sciences, State University of New York at Buffalo, 14214, USA
    J Bacteriol 187:7607-18. 2005
    ....
  16. pmc The RecF protein antagonizes RecX function via direct interaction
    Shelley L Lusetti
    Department of Biochemistry, University of Wisconsin, Madison, Madison, Wisconsin 53706, USA
    Mol Cell 21:41-50. 2006
    ..Thus, a significant positive contribution of RecF to RecA filament assembly is to antagonize the effects of the negative modulator RecX, specifically during the extension phase...
  17. pmc Homologous recombination prevents methylation-induced toxicity in Escherichia coli
    Anetta Nowosielska
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, 364 Plantation Street, Worcester, MA 01605, USA
    Nucleic Acids Res 34:2258-68. 2006
    ....
  18. ncbi Altered SOS induction associated with mutations in recF, recO and recR
    M C Whitby
    Department of Genetics, University of Nottingham, Queens Medical Centre, UK
    Mol Gen Genet 246:174-9. 1995
    ..We show that normal induction also requires the products of recO and recR. SOS induction was monitored using a sfiA-lacZ fusion strain...
  19. pmc Roles of PriA protein and double-strand DNA break repair functions in UV-induced restriction alleviation in Escherichia coli
    Ivana Ivancić-Bacće
    Department of Molecular Biology, Faculty of Science, University of Zagreb, Croatia
    Genetics 174:2137-49. 2006
    ..On the basis of our results, we propose that unmethylated target sequences are generated at the D-loop by the strand exchange of two hemi-methylated duplex DNAs and subsequent initiation of DNA replication...
  20. pmc RecFOR proteins are essential for Pol V-mediated translesion synthesis and mutagenesis
    Shingo Fujii
    Genome Instability and Carcinogenesis, CNRS, FRE 2931, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
    EMBO J 25:5754-63. 2006
    ..Mutations in recF, recO or recR gene abolish induced mutagenesis in E. coli.
  21. ncbi SSB protein limits RecOR binding onto single-stranded DNA
    Michael D Hobbs
    Department of Biochemistry, University of Wisconsin, Madison, Wisconsin 53706 1544, USA
    J Biol Chem 282:11058-67. 2007
    The RecO and RecR proteins form a complex that promotes the nucleation of RecA protein filaments onto SSB protein-coated single-stranded DNA (ssDNA)...
  22. pmc UvrD and UvrD252 counteract RecQ, RecJ, and RecFOR in a rep mutant of Escherichia coli
    Roxane Lestini
    CNRS, Centre de Genetique Moleculaire, UPR 2167, Gif sur Yvette F 91198, Universite Paris Sud, Orsay F 91405, France
    J Bacteriol 190:5995-6001. 2008
    ..of rep and uvrD inactivation is suppressed in the absence of the recombination presynaptic proteins RecF, RecO, or RecR, it was proposed that UvrD is essential in the rep mutant to counteract a deleterious RecFOR-dependent RecA binding...
  23. doi Maintaining replication fork integrity in UV-irradiated Escherichia coli cells
    Christian J Rudolph
    Institute of Genetics, University of Nottingham, Queen s Medical Centre, Nottingham, United Kingdom
    DNA Repair (Amst) 7:1589-602. 2008
    ..Instead, nascent strands may be protected by the simple expedient of promoting restart. We also report that the efficiency of fork reactivation is not affected in polB mutants...
  24. pmc Reconstitution of initial steps of dsDNA break repair by the RecF pathway of E. coli
    Naofumi Handa
    Department of Microbiology, University of California at Davis, Davis, Calfironia 95616, USA
    Genes Dev 23:1234-45. 2009
    ..a seven-protein reaction that recapitulates early steps of dsDNA break repair using purified RecA, RecF, RecO, RecR, RecQ, RecJ, and SSB proteins, components of the RecF system...
  25. pmc Sequence and expression of the Escherichia coli recR locus
    T Yeung
    Department of Microbiology, University of Texas, Austin 78712
    J Bacteriol 172:6042-7. 1990
    The Escherichia coli RecR protein participates in a recombinational DNA repair process...
  26. pmc Participation of recombination proteins in rescue of arrested replication forks in UV-irradiated Escherichia coli need not involve recombination
    J Courcelle
    Department of Biological Sciences, Mississippi State University, P O Box GY, Mississippi State, MS 39762 5759, USA
    Proc Natl Acad Sci U S A 98:8196-202. 2001
    ..Following UV irradiation, the recovery of DNA replication requires uvrA and uvrC, as well as recA, recF, and recR. The rec genes appear to be required to protect and maintain replication forks that are arrested at DNA lesions, ..
  27. pmc Biochemistry of homologous recombination in Escherichia coli
    S C Kowalczykowski
    Division of Biological Sciences, University of California, Davis 95616 8665
    Microbiol Rev 58:401-65. 1994
    ..This review focuses on the biochemical mechanisms underlying these steps, with particular emphases on the activities of the proteins involved and on the integration of these activities into likely biochemical pathways for recombination...
  28. ncbi Homologous genetic recombination: the pieces begin to fall into place
    A J Clark
    Department of Molecular and Cell Biology, University of California, Berkeley 94720 3202
    Crit Rev Microbiol 20:125-42. 1994
    ..This in turn led to the discovery of several genes, including recF, recO, and recR, whose role in recombination would not otherwise have been detected...
  29. pmc Biochemical interaction of the Escherichia coli RecF, RecO, and RecR proteins with RecA protein and single-stranded DNA binding protein
    K Umezu
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115
    Proc Natl Acad Sci U S A 90:3875-9. 1993
    The Escherichia coli RecF, RecO, and RecR proteins were analyzed for their effect on RecA-mediated pairing of single-stranded circular DNA and homologous linear duplex DNA substrates...
  30. ncbi An interaction between the Escherichia coli RecF and RecR proteins dependent on ATP and double-stranded DNA
    B L Webb
    Department of Biochemistry, University of Wisconsin Madison 53706, USA
    J Biol Chem 270:31397-404. 1995
    The DNA binding and ATPase activities of RecF protein are modulated by RecR protein...
  31. ncbi RecA protein filaments: end-dependent dissociation from ssDNA and stabilization by RecO and RecR proteins
    Q Shan
    Department of Biochemistry, University of Wisconsin Madison 53706, USA
    J Mol Biol 265:519-40. 1997
    ..3 or when dATP replaces ATP. Disassembly is not observed even with ATP when both the RecO and RecR proteins are present in the initial reaction mixture...
  32. pmc recF and recR are required for the resumption of replication at DNA replication forks in Escherichia coli
    J Courcelle
    Department of Biological Sciences, Stanford University, CA 94305 5020, USA
    Proc Natl Acad Sci U S A 94:3714-9. 1997
    ..Here, we show that when replication is disrupted, at least two genes in the recF pathway, recF and recR, are required for the resumption of replication at DNA replication forks, and that in their absence, localized ..
  33. ncbi Recombinational DNA repair: the RecF and RecR proteins limit the extension of RecA filaments beyond single-strand DNA gaps
    B L Webb
    Department of Biochemistry, University of Wisconsin at Madison, 53706, USA
    Cell 91:347-56. 1997
    In the presence of both the RecF and RecR proteins, RecA filament extension from a single strand gap into adjoining duplex DNA is attenuated. RecR protein alone has no effect, and RecF protein alone has a reduced activity...
  34. ncbi Resolution of holliday junctions by RuvABC prevents dimer formation in rep mutants and UV-irradiated cells
    B Michel
    Génétique Microbienne, INRA, 78352 Jouy en Josas Cedex, France
    Mol Microbiol 37:180-91. 2000
    ..We conclude that the inviability arising from a high frequency of dimer formation in rep or UV-irradiated cells is only observed in the absence of known enzymes that resolve Holliday junctions...
  35. ncbi Lethality of bypass polymerases in Escherichia coli cells with a defective clamp loader complex of DNA polymerase III
    Enrique Viguera
    Génétique Microbienne, Institut National de la Recherche Agronomique, 78350 Jouy en Josas, France
    Mol Microbiol 50:193-204. 2003
    ....
  36. ncbi Recombinational repair of chromosomal DNA double-strand breaks generated by a restriction endonuclease
    G A Cromie
    Institute of Cell and Molecular Biology, University of Edinburgh, Kings Buildings, Edinburgh EH9 3JR, UK
    Mol Microbiol 41:873-83. 2001
    ....
  37. pmc The RecOR proteins modulate RecA protein function at 5' ends of single-stranded DNA
    J M Bork
    Department of Biochemistry, University of Wisconsin Madison, Madison, WI 53706 1544, USA
    EMBO J 20:7313-22. 2001
    The Escherichia coli RecF, RecO and RecR pro teins have previously been implicated in bacterial recombinational DNA repair at DNA gaps...
  38. ncbi Replication restart in UV-irradiated Escherichia coli involving pols II, III, V, PriA, RecA and RecFOR proteins
    Savithri Rangarajan
    Department of Biological Sciences and Chemistry, Hedco Molecular Biology Laboratories, University of Southern California, Los Angeles, CA 90089 1340, USA
    Mol Microbiol 43:617-28. 2002
    ..strains carrying a combination of mutations in lexA, recA, polB (pol II), umuDC (pol V), priA, dnaC, recF, recO or recR. We find that both pol II and the origin-independent primosome-assembling function of PriA are essential for the ..
  39. pmc Roles of RecJ, RecO, and RecR in RecET-mediated illegitimate recombination in Escherichia coli
    Kouya Shiraishi
    Center for Basic Research, The Kitasato Institute, Shirokane 5 9 1, Minato ku, Tokyo 108 8642, Japan
    J Bacteriol 184:4715-21. 2002
    ..by coexpression of RecE and RecT in the wild type, but the enhanced recombination was reduced by recJ, recO, or recR mutation...
  40. pmc Escherichia coli RecO protein anneals ssDNA complexed with its cognate ssDNA-binding protein: A common step in genetic recombination
    Noriko Kantake
    Sections of Microbiology and Molecular and Cellular Biology, Genetics Graduate Group, University of California, Davis 95616, USA
    Proc Natl Acad Sci U S A 99:15327-32. 2002
    ..Optimal activity for RecO protein occurs after formation of a 1:1 complex with SSB protein. RecR protein, which is known to stimulate RecO protein to facilitate SSB protein displacement by RecA protein, inhibits ..
  41. ncbi PriA mediates DNA replication pathway choice at recombination intermediates
    Liewei Xu
    Molecular Biology Program, Memorial Sloan Kettering Cancer Center, New York, NY 10021, USA
    Mol Cell 11:817-26. 2003
    ..Furthermore, we find that RecO and RecR significantly stimulate this recombination-directed DNA replication reaction, and that this stimulation is ..
  42. pmc Accumulation of large non-circular forms of the chromosome in recombination-defective mutants of Escherichia coli
    Naofumi Handa
    Division of Molecular Biology, Institute of Medical Science, University of Tokyo, Shirokanedai, Tokyo 108 8639 Japan
    BMC Mol Biol 4:5. 2003
    ..However, recent experiments suggest that such breakage may occur frequently during "normal" growth in various organisms - from bacteria through vertebrates, possibly through arrest of a replication fork at some endogenous DNA damage...
  43. ncbi Protein interactions in genetic recombination in Escherichia coli. Interactions involving RecO and RecR overcome the inhibition of RecA by single-stranded DNA-binding protein
    K Umezu
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115
    J Biol Chem 269:30005-13. 1994
    ..However, RecO and RecR can act together as accessory factors for RecA to overcome this inhibition by SSB (Umezu, K., Chi, N.-W...