recF

Summary

Gene Symbol: recF
Description: gap repair protein
Alias: ECK3692, JW3677, uvrF
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. ncbi Genetic analysis of UV mutagenesis of the Escherichia coli glyU gene
    Z Ciesla
    Mol Gen Genet 207:1-8. 1987
  2. pmc his operons of Escherichia coli and Salmonella typhimurium are regulated by DNA supercoiling
    K E Rudd
    Proc Natl Acad Sci U S A 84:517-21. 1987
  3. ncbi Involvement of recA and recF in the induced precise excision of Tn10 in Escherichia coli
    A Chan
    CEFYBO, CONICET, Buenos Aires, Argentina
    Mutat Res 381:111-5. 1997
  4. ncbi RecFOR proteins load RecA protein onto gapped DNA to accelerate DNA strand exchange: a universal step of recombinational repair
    Katsumi Morimatsu
    Section of Microbiology, Center for Genetics and Development, University of California, Davis, 95616, USA
    Mol Cell 11:1337-47. 2003
  5. ncbi Requirement for RecFOR-mediated recombination in priA mutant
    Gianfranco Grompone
    Génétique Microbienne, Institut National de la Recherche Agronomique, Domaine de Vilvert 78350 Jouy en Josas, France
    Mol Microbiol 52:551-62. 2004
  6. ncbi PriA mediates DNA replication pathway choice at recombination intermediates
    Liewei Xu
    Molecular Biology Program, Memorial Sloan Kettering Cancer Center, New York, NY 10021, USA
    Mol Cell 11:817-26. 2003
  7. pmc recF and recR are required for the resumption of replication at DNA replication forks in Escherichia coli
    J Courcelle
    Department of Biological Sciences, Stanford University, CA 94305 5020, USA
    Proc Natl Acad Sci U S A 94:3714-9. 1997
  8. ncbi Spontaneous and cisplatin-induced recombination in Escherichia coli
    Anetta Nowosielska
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, 364 Plantation Street, LRB823 Worcester, MA 01655, USA
    DNA Repair (Amst) 3:719-28. 2004
  9. pmc Biochemistry of homologous recombination in Escherichia coli
    S C Kowalczykowski
    Division of Biological Sciences, University of California, Davis 95616 8665
    Microbiol Rev 58:401-65. 1994
  10. ncbi Replication restart: a pathway for (CTG).(CAG) repeat deletion in Escherichia coli
    Seung Hwan Kim
    Laboratory of DNA Structure and Mutagenesis, Center for Genome Research, Institute of Biosciences and Technology, Texas A and M University System Health Science Center, 2121 West Holcombe Blvd, Houston, TX 77030 3303, USA
    Mutat Res 595:5-22. 2006

Scientific Experts

  • Rajesh Sharan
  • Marc Bichara
  • Xavier Veaute
  • J Courcelle
  • Hideo Ikeda
  • Kin Hoe Chow
  • Naofumi Handa
  • Qais Al-Hadid
  • Katherine R Ona
  • Kevin Hiom
  • V V Sukhodelets
  • E Adam
  • Anetta Nowosielska
  • Bénédicte Michel
  • B L Webb
  • B Michel
  • Christian J Rudolph
  • Roxane Lestini
  • Daniel B Magner
  • Laura M Pollard
  • Elena Lopez
  • Ivana Ivancić-Bacće
  • Bryony T I Payne
  • Michael M Cox
  • M M Cox
  • Seung Hwan Kim
  • Quan Lin
  • Shingo Fujii
  • A J Clark
  • Shelley L Lusetti
  • Ksenija Zahradka
  • Dmitry M Baitin
  • R B Inman
  • Erika Salaj-Smic
  • Krunoslav Brcic-Kostic
  • Micheal L Hebert
  • Ivana Ivancic-Bace
  • M G Marinus
  • Davor Zahradka
  • S J Sandler
  • Mirjana Petranovic
  • John M Essigmann
  • Gianfranco Grompone
  • Jessica L Kosa
  • Takeharu Yamanaka
  • M Seigneur
  • Katsumi Morimatsu
  • Enrique Viguera
  • Aline V Grigorian
  • Liewei Xu
  • S P Hegde
  • Savithri Rangarajan
  • S Dusko Ehrlich
  • G A Cromie
  • V V Sukhodolets
  • J M Bork
  • S D Ehrlich
  • M V Madiraju
  • M Penel-Colin
  • Amy L Upton
  • Robert G Lloyd
  • V Bidnenko
  • James R Lupski
  • Marina Elez
  • Paul M Rindler
  • Ivan Matic
  • Jennifer A Lee
  • Sanjay I Bidichandani
  • Jeanine M Pennington
  • Yogesh K Chutake
  • Jesus Blazquez
  • Susan M Rosenberg
  • Matthew D Blankschien
  • Małgorzata J Pytlos
  • Gordana Cogelja-Cajo
  • Sanela Simic
  • Stephen A Smith
  • Yasuko Rikihisa
  • Damir Dermic
  • Elizabeth A Stohl
  • David J Sherratt
  • Michael D Hobbs
  • Sergio R Filipe
  • Robert P Fuchs
  • Peter McGlynn
  • Asako Isogawa
  • Maja Buljubasic
  • Ignacija Vlasic
  • Sindhu Chitteni-Pattu
  • Ingeborg C van Knippenberg

Detail Information

Publications84

  1. ncbi Genetic analysis of UV mutagenesis of the Escherichia coli glyU gene
    Z Ciesla
    Mol Gen Genet 207:1-8. 1987
    ..When carried by M13 phage mp9, glyU is subject to induced UV mutagenesis which is dependent on the umuC+ and recF+ genes. When carried by M13 phage mp8, glyU is not subject to induced UV mutagenesis...
  2. pmc his operons of Escherichia coli and Salmonella typhimurium are regulated by DNA supercoiling
    K E Rudd
    Proc Natl Acad Sci U S A 84:517-21. 1987
    ..Several lines of evidence, including high transduction linkage to recF, suggest that hisU1820 is an allele of gyrB. Finally, well-characterized gyrA and gyrB alleles of E...
  3. ncbi Involvement of recA and recF in the induced precise excision of Tn10 in Escherichia coli
    A Chan
    CEFYBO, CONICET, Buenos Aires, Argentina
    Mutat Res 381:111-5. 1997
    ..The effect of a null recF mutation on the excision of Tn10 in a uvrD- mutant was also investigated and found to abolish the increased ..
  4. ncbi RecFOR proteins load RecA protein onto gapped DNA to accelerate DNA strand exchange: a universal step of recombinational repair
    Katsumi Morimatsu
    Section of Microbiology, Center for Genetics and Development, University of California, Davis, 95616, USA
    Mol Cell 11:1337-47. 2003
    Genetic evidence suggests that the RecF, RecO, and RecR (RecFOR) proteins participate in a common step of DNA recombination and repair, yet the biochemical event requiring collaboration of all three proteins is unknown...
  5. ncbi Requirement for RecFOR-mediated recombination in priA mutant
    Gianfranco Grompone
    Génétique Microbienne, Institut National de la Recherche Agronomique, Domaine de Vilvert 78350 Jouy en Josas, France
    Mol Microbiol 52:551-62. 2004
    ..Models for the formation of single strand DNA gaps consequently to a replication restart defect and for gap processing are proposed...
  6. ncbi PriA mediates DNA replication pathway choice at recombination intermediates
    Liewei Xu
    Molecular Biology Program, Memorial Sloan Kettering Cancer Center, New York, NY 10021, USA
    Mol Cell 11:817-26. 2003
    ..this recombination-directed DNA replication reaction, and that this stimulation is modulated by the presence of RecF, suggesting that the latter protein may also act as a regulator of the pathway of resolution of the recombination ..
  7. pmc recF and recR are required for the resumption of replication at DNA replication forks in Escherichia coli
    J Courcelle
    Department of Biological Sciences, Stanford University, CA 94305 5020, USA
    Proc Natl Acad Sci U S A 94:3714-9. 1997
    Escherichia coli containing a mutation in recF are hypersensitive to UV. However, they exhibit normal levels of conjugational or transductional recombination unless the major pathway (recBC) is defective...
  8. ncbi Spontaneous and cisplatin-induced recombination in Escherichia coli
    Anetta Nowosielska
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, 364 Plantation Street, LRB823 Worcester, MA 01655, USA
    DNA Repair (Amst) 3:719-28. 2004
    ..Inactivation of the recF, recO, recR and recJ genes decreased cisplatin-induced, but not spontaneous, recombination...
  9. pmc Biochemistry of homologous recombination in Escherichia coli
    S C Kowalczykowski
    Division of Biological Sciences, University of California, Davis 95616 8665
    Microbiol Rev 58:401-65. 1994
    ..At present, this includes the RecA, RecBCD (exonuclease V), RecE (exonuclease VIII), RecF, RecG, RecJ, RecN, RecOR, RecQ, RecT, RuvAB, RuvC, SbcCD, and SSB proteins, as well as DNA polymerase I, DNA gyrase,..
  10. ncbi Replication restart: a pathway for (CTG).(CAG) repeat deletion in Escherichia coli
    Seung Hwan Kim
    Laboratory of DNA Structure and Mutagenesis, Center for Genome Research, Institute of Biosciences and Technology, Texas A and M University System Health Science Center, 2121 West Holcombe Blvd, Houston, TX 77030 3303, USA
    Mutat Res 595:5-22. 2006
    ..Mutations in priB and priC resulted in small reductions in deletion rates. In a recF strain, rates were decreased when (CAG)n comprised the leading template strand, but rates were increased when (CTG)..
  11. pmc Genetic analysis of the RecE pathway of genetic recombination in Escherichia coli K-12
    J R Gillen
    J Bacteriol 145:521-32. 1981
    ..In addition, strain construction and mutagenesis revealed the dependence of the RecE pathway on recA+ and on recF+. Dependence on a fourth gene was shown by a mutation (rec-77) which does not map near the other genes...
  12. doi DNA repair: common approaches to fixing double-strand breaks
    Kevin Hiom
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 0QH, UK
    Curr Biol 19:R523-5. 2009
    In bacteria, the RecF pathway plays an important role in the repair of DNA breaks and gaps. Reconstitution of this reaction in vitro has revealed similarities with double-strand break repair in eukaryotes.
  13. ncbi Replication restart in UV-irradiated Escherichia coli involving pols II, III, V, PriA, RecA and RecFOR proteins
    Savithri Rangarajan
    Department of Biological Sciences and Chemistry, Hedco Molecular Biology Laboratories, University of Southern California, Los Angeles, CA 90089 1340, USA
    Mol Microbiol 43:617-28. 2002
    ..restart in strains carrying a combination of mutations in lexA, recA, polB (pol II), umuDC (pol V), priA, dnaC, recF, recO or recR...
  14. ncbi Resolution of holliday junctions by RuvABC prevents dimer formation in rep mutants and UV-irradiated cells
    B Michel
    Génétique Microbienne, INRA, 78352 Jouy en Josas Cedex, France
    Mol Microbiol 37:180-91. 2000
    ..We conclude that the inviability arising from a high frequency of dimer formation in rep or UV-irradiated cells is only observed in the absence of known enzymes that resolve Holliday junctions...
  15. ncbi ATP hydrolysis and DNA binding by the Escherichia coli RecF protein
    B L Webb
    Department of Biochemistry, University of Wisconsin Madison, Madison, Wisconsin 53706, USA
    J Biol Chem 274:15367-74. 1999
    The Escherichia coli RecF protein possesses a weak ATP hydrolytic activity. ATP hydrolysis leads to RecF dissociation from double-stranded (ds)DNA...
  16. ncbi An interaction between the Escherichia coli RecF and RecR proteins dependent on ATP and double-stranded DNA
    B L Webb
    Department of Biochemistry, University of Wisconsin Madison 53706, USA
    J Biol Chem 270:31397-404. 1995
    The DNA binding and ATPase activities of RecF protein are modulated by RecR protein...
  17. pmc UvrD helicase, unlike Rep helicase, dismantles RecA nucleoprotein filaments in Escherichia coli
    Xavier Veaute
    CEA, DSV, DRR, UMR217 CNRS CEA, Fontenay aux Roses, France
    EMBO J 24:180-9. 2005
    ..We reported earlier that mutations in recF, recO or recR genes suppress the lethality of uvrD rep, and proposed that an essential activity common to UvrD and ..
  18. pmc Roles of PriA protein and double-strand DNA break repair functions in UV-induced restriction alleviation in Escherichia coli
    Ivana Ivancić-Bacće
    Department of Molecular Biology, Faculty of Science, University of Zagreb, Croatia
    Genetics 174:2137-49. 2006
    ..On the basis of our results, we propose that unmethylated target sequences are generated at the D-loop by the strand exchange of two hemi-methylated duplex DNAs and subsequent initiation of DNA replication...
  19. doi RecA433 cells are defective in recF-mediated processing of disrupted replication forks but retain recBCD-mediated functions
    Qais Al-Hadid
    Department of Biology, Portland State University, Portland, OR 97207, USA
    Mutat Res 645:19-26. 2008
    ..The cellular functions carried out by RecA require either recF pathway proteins or recBC pathway proteins to initiate RecA-loading onto the appropriate DNA substrates...
  20. pmc Deficiency of RecA-dependent RecFOR and RecBCD pathways causes increased instability of the (GAA*TTC)n sequence when GAA is the lagging strand template
    Laura M Pollard
    Department of Biochemistry and Molecular Biology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA
    Nucleic Acids Res 35:6884-94. 2007
    ..Our data implicate defective processing of stalled replication forks as a mechanism for genetic instability of the (GAA*TTC)n sequence...
  21. pmc UvrD and UvrD252 counteract RecQ, RecJ, and RecFOR in a rep mutant of Escherichia coli
    Roxane Lestini
    CNRS, Centre de Genetique Moleculaire, UPR 2167, Gif sur Yvette F 91198, Universite Paris Sud, Orsay F 91405, France
    J Bacteriol 190:5995-6001. 2008
    ..lethality of rep and uvrD inactivation is suppressed in the absence of the recombination presynaptic proteins RecF, RecO, or RecR, it was proposed that UvrD is essential in the rep mutant to counteract a deleterious RecFOR-..
  22. pmc Interactions of RecF protein with RecO, RecR, and single-stranded DNA binding proteins reveal roles for the RecF-RecO-RecR complex in DNA repair and recombination
    S P Hegde
    Department of Microbiology, University of Texas Health Center at Tyler 75710, USA
    Proc Natl Acad Sci U S A 93:14468-73. 1996
    The products of the recF, recO, and recR genes are thought to interact and assist RecA in the utilization of single-stranded DNA precomplexed with single-stranded DNA binding protein (Ssb) during synapsis...
  23. pmc Preferential binding of Escherichia coli RecF protein to gapped DNA in the presence of adenosine (gamma-thio) triphosphate
    S P Hegde
    Department of Microbiology, University of Texas Health Center at Tyler 75710, USA
    J Bacteriol 178:184-90. 1996
    Escherichia coli RecF protein binds, but does not hydrolyze, ATP...
  24. ncbi RecN and RecG are required for Escherichia coli survival of Bleomycin-induced damage
    Jessica L Kosa
    Biological Engineering Division, Department of Chemistry, Massachusetts Institute of Technology, Rm 56 689, 77 Massachusetts Avenue, Cambridge 02139, USA
    Mutat Res 554:149-57. 2004
    ..and recN, which lack proteins required for double strand break (DSB) repair, were highly sensitive to BLM, while recF cells were not...
  25. ncbi [Homologous recombination and chromosomal rearrangements in Escherichia coli strains carrying a heterozygous tandem duplication]
    V V Sukhodolets
    State Research Institute of Genetics and Selection of Industrial Microorganisms, Moscow, 113545 Russia
    Genetika 37:591-601. 2001
    ..recombination between homologous DNA repeats was very high in all strains and did not decrease when the RecBCD and RecF recombinational pathways were simultaneously damaged in strains with the recB sbcBC recQ (or recF) genotype...
  26. pmc Nucleotide excision repair is a predominant mechanism for processing nitrofurazone-induced DNA damage in Escherichia coli
    Katherine R Ona
    Department of Biology, Portland State University, Portland, OR 97201, USA
    J Bacteriol 191:4959-65. 2009
    ..In addition, survival also depended on both the RecF and RecBCD pathways...
  27. pmc Escherichia coli cells with increased levels of DnaA and deficient in recombinational repair have decreased viability
    Aline V Grigorian
    Department of Biology, San Diego State University, California 92182 4614, USA
    J Bacteriol 185:630-44. 2003
    The dnaA operon of Escherichia coli contains the genes dnaA, dnaN, and recF encoding DnaA, beta clamp of DNA polymerase III holoenzyme, and RecF...
  28. pmc Reconstitution of initial steps of dsDNA break repair by the RecF pathway of E. coli
    Naofumi Handa
    Department of Microbiology, University of California at Davis, Davis, Calfironia 95616, USA
    Genes Dev 23:1234-45. 2009
    The RecF pathway of Escherichia coli is important for recombinational repair of DNA breaks and gaps...
  29. doi Contribution of RecFOR machinery of homologous recombination to cell survival after loss of a restriction-modification gene complex
    Naofumi Handa
    Laboratory of Social Genome Sciences, Department of Medical Genome Sciences, Graduate School of Frontier Sciences, University of Tokyo, 4 6 1 Shirokanedai, Minato ku, Tokyo 108 8639, Japan
    Microbiology 155:2320-32. 2009
    ..A recF mutation reduced survival in an otherwise rec-positive background and, more severely, in a recBC sbcBC background...
  30. pmc Accumulation of large non-circular forms of the chromosome in recombination-defective mutants of Escherichia coli
    Naofumi Handa
    Division of Molecular Biology, Institute of Medical Science, University of Tokyo, Shirokanedai, Tokyo 108 8639 Japan
    BMC Mol Biol 4:5. 2003
    ..However, recent experiments suggest that such breakage may occur frequently during "normal" growth in various organisms - from bacteria through vertebrates, possibly through arrest of a replication fork at some endogenous DNA damage...
  31. doi Preferential post-replication repair of DNA lesions situated on the leading strand of plasmids in Escherichia coli
    Marc Bichara
    Université Strasbourg 1, Institut Gillbert Laustrait, CNRS UMR 7175 Boulevard Sebastien Brant, BP 10413, F 67412 Illkirch cedex, France
    Mol Microbiol 71:305-14. 2009
    In Escherichia coli, RecF-dependent post-replication repair (PRR) permits cells to tolerate the potentially lethal effects of blocking lesions at the replication fork...
  32. ncbi Lethality of bypass polymerases in Escherichia coli cells with a defective clamp loader complex of DNA polymerase III
    Enrique Viguera
    Génétique Microbienne, Institut National de la Recherche Agronomique, 78350 Jouy en Josas, France
    Mol Microbiol 50:193-204. 2003
    ....
  33. doi Maintaining replication fork integrity in UV-irradiated Escherichia coli cells
    Christian J Rudolph
    Institute of Genetics, University of Nottingham, Queen s Medical Centre, Nottingham, United Kingdom
    DNA Repair (Amst) 7:1589-602. 2008
    ..Instead, nascent strands may be protected by the simple expedient of promoting restart. We also report that the efficiency of fork reactivation is not affected in polB mutants...
  34. pmc RecFOR proteins are essential for Pol V-mediated translesion synthesis and mutagenesis
    Shingo Fujii
    Genome Instability and Carcinogenesis, CNRS, FRE 2931, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
    EMBO J 25:5754-63. 2006
    ..A positive contribution of RecF protein to Pol V lesion bypass is demonstrated...
  35. ncbi Illegitimate recombination mediated by double-strand break and end-joining in Escherichia coli
    Hideo Ikeda
    Institute of Molecular Genetics, Medinet Inc, Tamagawadai 2 2 8, Setagaya Ku, Tokyo 158 0096, Japan
    Adv Biophys 38:3-20. 2004
    ..RecE and RecT enhances the frequencies of spontaneous and UV-induced illegitimate recombination and that the RecJ, RecF, RecO, and RecR functions are required for this RecE-mediated illegitimate recombination...
  36. ncbi Illegitimate recombination mediated by double-strand break and end-joining in Escherichia coli
    Hideo Ikeda
    Institute of Molecular Genetics, Medinet Inc, Tamagawadai 2 2 8, Setagaya Ku, Tokyo 158 0096, Japan
    Adv Biophys 38:3-20. 2004
    ..RecE and RecT enhances the frequencies of spontaneous and UV-induced illegitimate recombination and that the RecJ, RecF, RecO, and RecR functions are required for this RecE-mediated illegitimate recombination...
  37. pmc RecQ promotes toxic recombination in cells lacking recombination intermediate-removal proteins
    Daniel B Magner
    Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX 77030, USA
    Mol Cell 26:273-86. 2007
    ..Death and segregation failure require RecA and RecF strand exchange proteins...
  38. pmc The TAO-Gen algorithm for identifying gene interaction networks with application to SOS repair in E. coli
    Takeharu Yamanaka
    Laboratory of Computational Biology and Risk Analysis, National Institute of Environmental Health Sciences, National Institutes of Health DHHS, 111 Alexander Drive, Research Triangle Park, NC 27709, USA
    Environ Health Perspect 112:1614-21. 2004
    ..The method is used to evaluate interactions between genes in the SOS signaling pathway in Escherichia coli using gene expression data where each gene in the network is over-expressed using plasmids inserts...
  39. pmc Effects of recJ, recQ, and recFOR mutations on recombination in nuclease-deficient recB recD double mutants of Escherichia coli
    Ivana Ivancic-Bace
    Department of Molecular Biology, Ruder Boskovic Institute, Bijenicka 54, HR 10002 Zagreb, Croatia
    J Bacteriol 187:1350-6. 2005
    The two main recombination pathways in Escherichia coli (RecBCD and RecF) have different recombination machineries that act independently in the initiation of recombination...
  40. ncbi Recs preventing wrecks
    Justin Courcelle
    Portland State University, Department of Biology, P O Box 751, Portland, OR 97207 0751, USA
    Mutat Res 577:217-27. 2005
    ..When this process is prematurely disrupted by DNA damage, several recF pathway gene products play critical roles processing the arrested replication fork, allowing it to resume and ..
  41. pmc The RecF protein antagonizes RecX function via direct interaction
    Shelley L Lusetti
    Department of Biochemistry, University of Wisconsin, Madison, Madison, Wisconsin 53706, USA
    Mol Cell 21:41-50. 2006
    The RecX protein inhibits RecA filament extension, leading to net filament disassembly. The RecF protein physically interacts with the RecX protein and protects RecA from the inhibitory effects of RecX...
  42. ncbi Antibiotic-mediated recombination: ciprofloxacin stimulates SOS-independent recombination of divergent sequences in Escherichia coli
    Elena Lopez
    Departamento de Biotecnologia Microbiana, Centro Nacional de Biotecnologia CSIC, Campus UAM Cantoblanco, 28049 Madrid, Spain
    Mol Microbiol 64:83-93. 2007
    ....
  43. pmc Analysis of involvement of the RecF pathway in p44 recombination in Anaplasma phagocytophilum and in Escherichia coli by using a plasmid carrying the p44 expression and p44 donor loci
    Quan Lin
    Department of Veterinary Biosciences, College of Veterinary Medicine, The Ohio State University, 1925 Coffey Road, Columbus, OH 43210, USA
    Infect Immun 74:2052-62. 2006
    ..Homologs of genes for the RecA-dependent RecF pathway, but not the RecBCD or RecE pathways, of recombination were detected in the A. phagocytophilum genome...
  44. ncbi Critical role of RecA and RecF proteins in strand break rejoining and maintenance of fidelity of rejoining following gamma-radiation-induced damage to pMTa4 DNA in E. coli
    R N Sharan
    Department of Radiation Biology and Medical Genetics, Graduate School of Medicine, Osaka University, Osaka, Japan
    Int J Radiat Biol 83:89-97. 2007
    This study was undertaken to understand the roles of RecA and RecF proteins in strand break rejoining and maintenance of fidelity of the process following exposure of E. coli to gamma-radiation in vivo.
  45. pmc Homologous recombination prevents methylation-induced toxicity in Escherichia coli
    Anetta Nowosielska
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, 364 Plantation Street, Worcester, MA 01605, USA
    Nucleic Acids Res 34:2258-68. 2006
    ..We find that recombination-deficient cell lines, such as recBCD recF, and ruvC recG, are as sensitive to the cytotoxic effects of MMS and MNNG as the most base excision repair (BER)-..
  46. pmc Replication fork blockage by transcription factor-DNA complexes in Escherichia coli
    Bryony T I Payne
    School of Medical Sciences, Institute of Medical Sciences, University of Aberdeen, Foresterhill, Aberdeen AB25 2ZD, UK
    Nucleic Acids Res 34:5194-202. 2006
    ..However, neither RuvABC nor RecF were needed for normal cell growth in the face of such complexes...
  47. pmc Properties of a mutant recA-encoded protein reveal a possible role for Escherichia coli recF-encoded protein in genetic recombination
    M V Madiraju
    Department of Molecular Biology, University of California, Berkeley 94720
    Proc Natl Acad Sci U S A 85:6592-6. 1988
    ..DNA binding protein is the property that allows recA803 to suppress partially the deficiency in repair caused by recF mutations in the uvrA6 background...
  48. pmc Participation of recombination proteins in rescue of arrested replication forks in UV-irradiated Escherichia coli need not involve recombination
    J Courcelle
    Department of Biological Sciences, Mississippi State University, P O Box GY, Mississippi State, MS 39762 5759, USA
    Proc Natl Acad Sci U S A 98:8196-202. 2001
    ..Following UV irradiation, the recovery of DNA replication requires uvrA and uvrC, as well as recA, recF, and recR...
  49. pmc Genetic analysis of regulation of the RecF pathway of recombination in Escherichia coli K-12
    S T Lovett
    J Bacteriol 153:1471-8. 1983
    Genetic evidence is provided supporting the hypothesis that one or more genes of the RecF pathway of recombination other than recA are controlled by the lexA repressor...
  50. ncbi Role of the recF gene of Escherichia coli K-12 in lambda recombination
    M E Armengod
    Mol Gen Genet 181:497-504. 1981
    ..phage, which are unable to replicate, general recombination between phage and prophage depends on the bacterial recF gene. It has been shown that in E...
  51. ncbi Genetic and physical mapping of recF in Escherichia coli K-12
    L W Ream
    Mol Gen Genet 180:115-21. 1980
    Two factor transductional crosses place recF at approximately 82 min on the E. coli chromosome; recF is highly cotransducible with dnaA and gyrB (cou)...
  52. pmc DNA sequence and transcription of the region upstream of the E. coli gyrB gene
    T Adachi
    Nucleic Acids Res 12:6389-95. 1984
    We have determined the sequence of a 1498 base-pair region in E. coli that extends from within dnaN through recF and into the gyrB gene. An open reading frame of 1071 base pairs has been identified with the recF structural gene...
  53. pmc DNA sequence of the E. coli gyrB gene: application of a new sequencing strategy
    T Adachi
    Nucleic Acids Res 15:771-84. 1987
    ..A plausible terminator of gyrB transcription is located just beyond the structural gene...
  54. ncbi Discoordinate gene expression in the dnaA-dnaN operon of Escherichia coli
    A Quinones
    Wissenschaftsbereich Genetik, Martin Luther Universitat, Halle Saale, German Democratic Republic
    Mol Gen Genet 213:118-24. 1988
    ..These results are consistent with the hypothesis that although dnaA and dnaN are organized in an operon considerable discoordinate transcription can occur, thus uncoupling dnaN and dnaA regulation, when needed...
  55. ncbi Cloning and deletion mapping of the recF dnaN region of the Escherichia coli chromosome
    L W Ream
    Plasmid 10:101-10. 1983
    By cloning a 3.6-kb EcoRI fragment of the Escherichia coli chromosome with pBR322 we located more precisely recF relative to dnaN. By deletion mapping we localized functional recF to a 1...
  56. pmc Purification and preliminary characterization of the Escherichia coli K-12 recF protein
    T J Griffin
    Division of Cellular and Molecular Biology, Dana Farber Cancer Institute, Boston, Massachusetts
    J Bacteriol 172:6291-9. 1990
    The recF gene of Escherichia coli is known to encode an Mr-40,000 protein that is involved in DNA recombinationa nd postreplication DNA repair...
  57. ncbi Use of recA803, a partial suppressor of recF, to analyze the effects of the mutant Ssb (single-stranded DNA-binding) proteins in vivo and in vitro
    M V Madiraju
    Department of Molecular and Cell Biology, University of California, Berkeley 94720
    Mol Gen Genet 224:129-35. 1990
    ..that the ssb-1 and ssb-113 mutants exert some of their effects by interfering with the normal function of wild-type RecF protein...
  58. ncbi Cloning and preliminary characterization of srf-2020 and srf-801, the recF partial suppressor mutations which map in recA of Escherichia coli K-12
    T C Wang
    Department of Molecular Biology, Chang Gung Medical College, Kwei San, Tao Yuan, Taiwan
    Biochimie 73:335-40. 1991
    We have located the single nucleotide changes suffered in recA sequence of 2 recF partial suppressor mutations: srf-2020 at codon 121 and srf-801 at codon 257. srf-2020 changes codon 121 from threonine (ACC) to isoleucine (ATC)...
  59. ncbi Positive and negative regulatory elements in the dnaA-dnaN-recF operon of Escherichia coli
    I Perez-Roger
    Instituto de Investigaciones Citologicas, Valencia, Spain
    Biochimie 73:329-34. 1991
    The recF gene of E coli lies within a cluster of genes which play essential roles in DNA replication; the gene order is dnaA dnaN recF gyrB...
  60. pmc Effect of RecF protein on reactions catalyzed by RecA protein
    M V Madiraju
    Department of Molecular and Cell Biology, Barker GPBB ASU, University of California, Berkeley 94720
    Nucleic Acids Res 19:6295-300. 1991
    b>RecF protein is one of at least three single strand DNA (ssDNA) binding proteins which act in recombination and repair in Escherichia coli...
  61. pmc Constitutive and UV-mediated activation of RecA protein: combined effects of recA441 and recF143 mutations and of addition of nucleosides and adenine
    M Sassanfar
    Section of Biochemistry, Molecular and Cell Biology, Cornell University, Ithaca, New York 14853
    J Bacteriol 173:5869-75. 1991
    ..To characterize the role of RecF in SOS induction and RecA activation, we determined the effects of the recF143 mutation on the rate of RecA-..
  62. pmc Sequence and complementation analysis of recF genes from Escherichia coli, Salmonella typhimurium, Pseudomonas putida and Bacillus subtilis: evidence for an essential phosphate binding loop
    S J Sandler
    Department of Molecular and Cell Biology, University of California, Berkeley 94720
    Nucleic Acids Res 20:839-45. 1992
    We have compared the recF genes from Escherichia coli K-12, Salmonella typhimurium, Pseudomonas putida, and Bacillus subtilis at the DNA and amino acid sequence levels...
  63. ncbi Homologous genetic recombination: the pieces begin to fall into place
    A J Clark
    Department of Molecular and Cell Biology, University of California, Berkeley 94720 3202
    Crit Rev Microbiol 20:125-42. 1994
    ..This in turn led to the discovery of several genes, including recF, recO, and recR, whose role in recombination would not otherwise have been detected...
  64. pmc recD sbcB sbcD mutants are deficient in recombinational repair of UV lesions by RecBC
    M Seigneur
    Génétique Microbienne, Institut National de la Recherche Agronomique, 78352 Jouy en Josas Cedex, France
    J Bacteriol 181:6220-1. 1999
    In recD sbcB sbcD mutants, repair of UV-irradiated DNA is strongly RecF dependent, indicating that RecBC is inactive...
  65. ncbi sbcB sbcC null mutations allow RecF-mediated repair of arrested replication forks in rep recBC mutants
    V Bidnenko
    Génétique Microbienne, Institut National de la Recherche Agronomique, 78352 Jouy en Josas Cedex, France
    Mol Microbiol 33:846-57. 1999
    ..that the combination of sbcB sbcCD null mutations restores the viability to rep recBC mutants by activation of the RecF pathway of recombination...
  66. ncbi Gene conversion in the Escherichia coli RecF pathway: a successive half crossing-over model
    K Yamamoto
    Department of Bacteriology, Medical School, University of Tokyo, Japan
    Mol Gen Genet 234:1-13. 1992
    ..to analyze mechanisms of gene conversion and homologous recombination in an Escherichia coli strain with an active RecF pathway (recBC sbcBC), we first established in cells of this strain a plasmid carrying two mutant neo genes, each ..
  67. ncbi Cytochrome c biogenesis is involved in the transposon Tn5-mediated bleomycin resistance and the associated fitness effect in Escherichia coli
    E Adam
    Abteilung Mikrobiologie, Biozentrum der Universitat Basel, Switzerland
    Mol Microbiol 28:15-24. 1998
    ..Inactivation of potent DNA oxidants confers a better fitness to the bacterium carrying the transposon, suggesting a symbiotic relationship between host and transposon...
  68. ncbi Overlapping functions for recF and priA in cell viability and UV-inducible SOS expression are distinguished by dnaC809 in Escherichia coli K-12
    S J Sandler
    Department of Molecular and Cell Biology, University of California, Berkeley 94720 3202, USA
    Mol Microbiol 19:871-80. 1996
    The recF and priA genes have roles in DNA repair and homologous recombination. Mutations in these genes also cause decreases in cell viability and alterations in UV-inducible sulAp-lacZ (SOS) expression...
  69. pmc Molecular analysis of the recF gene of Escherichia coli
    M A Blanar
    Proc Natl Acad Sci U S A 81:4622-6. 1984
    We analyzed the nucleotide sequence of a 1.325-kilobase region of wild-type Escherichia coli containing a functional recF gene and six Tn3 mutations that inactivate recF...
  70. ncbi An improved vector system for constructing transcriptional lacZ fusions: analysis of regulation of the dnaA, dnaN, recF and gyrB genes of Escherichia coli
    F Macian
    Instituto de Investigaciones Citologicas, Fundacion Valenciana de Investigaciones Biomedicas, Valencia, Spain
    Gene 145:17-24. 1994
    ..We use this system to compare the transcriptional activities of the promoters present in the dnaA-dnaN-recF-gyrB cluster. The order of strength of these promoters is gyrB > dnaA > recF > dnaN...
  71. pmc Mutational analysis of sequences in the recF gene of Escherichia coli K-12 that affect expression
    S J Sandler
    Department of Molecular and Cell Biology, University of California, Berkeley 94720
    J Bacteriol 176:4011-6. 1994
    The level of translation of recF-lacZ fusions is reduced 20-fold by nucleotides 49 to 146 of recF...
  72. ncbi Protein interactions in genetic recombination in Escherichia coli. Interactions involving RecO and RecR overcome the inhibition of RecA by single-stranded DNA-binding protein
    K Umezu
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115
    J Biol Chem 269:30005-13. 1994
    ..To elucidate the mechanism that underlies this process, we examined protein-protein interactions between RecA, RecF, RecO, RecR, and SSB, and characterized the structure and activity of the ssDNA complexes formed with different ..
  73. pmc The RecF pathway of homologous recombination can mediate the initiation of DNA damage-inducible replication of the Escherichia coli chromosome
    T Asai
    Department of Cell Biology, University of New Mexico School of Medicine, Albuquerque 87131
    J Bacteriol 176:7113-4. 1994
    ..These results indicate that the RecF pathway of homologous recombination can also catalyze iSDR initiation.
  74. ncbi Altered SOS induction associated with mutations in recF, recO and recR
    M C Whitby
    Department of Genetics, University of Nottingham, Queens Medical Centre, UK
    Mol Gen Genet 246:174-9. 1995
    ..Induction of the SOS response is dependent on RecA and also on the product of recF. We show that normal induction also requires the products of recO and recR...
  75. ncbi Overlapping arrangement of the recF and dnaN operons of Escherichia coli; positive and negative control sequences
    M E Armengod
    Gene 43:183-96. 1986
    The recF gene of Escherichia coli controls one of the recombination pathways and UV sensitivity, but its precise function and expression pattern are still largely unknown...
  76. pmc sbcB15 And DeltasbcB mutations activate two types of recf recombination pathways in Escherichia coli
    Ksenija Zahradka
    Division of Molecular Biology, Ruder Boskovic Institute, Bijenicka 54, P O Box 180, 10002 Zagreb, Croatia
    J Bacteriol 188:7562-71. 2006
    ..This phenotype can be corrected by suppressor mutations in sbcB and sbcC(D) genes, which activate an alternative RecF pathway of recombination...
  77. pmc Distinguishing characteristics of hyperrecombinogenic RecA protein from Pseudomonas aeruginosa acting in Escherichia coli
    Dmitry M Baitin
    Division of Molecular and Radiation Biophysics, Petersburg Nuclear Physics Institute, Russian Academy of Sciences, Gatchina St Petersburg 188300, Russia
    J Bacteriol 188:5812-20. 2006
    ..Expression of RecA(Pa) also partially suppresses the effects of recF, recO, and recR mutations...
  78. ncbi Recombinational DNA repair: the RecF and RecR proteins limit the extension of RecA filaments beyond single-strand DNA gaps
    B L Webb
    Department of Biochemistry, University of Wisconsin at Madison, 53706, USA
    Cell 91:347-56. 1997
    In the presence of both the RecF and RecR proteins, RecA filament extension from a single strand gap into adjoining duplex DNA is attenuated. RecR protein alone has no effect, and RecF protein alone has a reduced activity...
  79. pmc Biochemical interaction of the Escherichia coli RecF, RecO, and RecR proteins with RecA protein and single-stranded DNA binding protein
    K Umezu
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115
    Proc Natl Acad Sci U S A 90:3875-9. 1993
    The Escherichia coli RecF, RecO, and RecR proteins were analyzed for their effect on RecA-mediated pairing of single-stranded circular DNA and homologous linear duplex DNA substrates...
  80. pmc The RecOR proteins modulate RecA protein function at 5' ends of single-stranded DNA
    J M Bork
    Department of Biochemistry, University of Wisconsin Madison, Madison, WI 53706 1544, USA
    EMBO J 20:7313-22. 2001
    The Escherichia coli RecF, RecO and RecR pro teins have previously been implicated in bacterial recombinational DNA repair at DNA gaps...
  81. ncbi Recombinational repair of chromosomal DNA double-strand breaks generated by a restriction endonuclease
    G A Cromie
    Institute of Cell and Molecular Biology, University of Edinburgh, Kings Buildings, Edinburgh EH9 3JR, UK
    Mol Microbiol 41:873-83. 2001
    ....
  82. ncbi rec genes and homologous recombination proteins in Escherichia coli
    A J Clark
    Department of Molecular and Cell Biology, Barker GPBB ASU, University of California, Berkeley 94720
    Biochimie 73:523-32. 1991
    ..The genes are usually grouped into three pathways named RecBCD, RecE and RecF for prominent genes which function in each...
  83. ncbi RecO acts with RecF and RecR to protect and maintain replication forks blocked by UV-induced DNA damage in Escherichia coli
    Kin Hoe Chow
    Department of Biological Science, Mississippi State University, Mississippi State, Mississippi 39762, USA
    J Biol Chem 279:3492-6. 2004
    In Escherichia coli, recF and recR are required to stabilize and maintain replication forks arrested by UV-induced DNA damage...
  84. ncbi Roles of double-strand breaks, nicks, and gaps in stimulating deletions of CTG.CAG repeats by intramolecular DNA repair
    Micheal L Hebert
    Center for Genome Research, Institute of Biosciences and Technology, Texas A and M University System Health Science Center, 2121 W Holcombe Blvd, Houston, TX 77030 3303, USA
    J Mol Biol 353:961-79. 2005
    ..Double-strand break-induced instabilities were partially stabilized by a mutation in recF. Gaps of 30 nt formed a distinct 30 nt deletion product, whereas single strand nicks and gaps of 15 nt did not ..