recA

Summary

Gene Symbol: recA
Description: DNA recombination and repair protein; ssDNA-dependent ATPase; synaptase; ssDNA and dsDNA binding protein; ATP-dependent homologous DNA strand exchanger; recombinase A; LexA autocleavage cofactor
Alias: ECK2694, JW2669, lexB, recH, rnmB, srf, tif, umuB, umuR, zab
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. ncbi An interaction between a specified surface of the C-terminal domain of RecA protein and double-stranded DNA for homologous pairing
    H Aihara
    The Institute of Physical and Chemical Research RIKEN, Wako Shi, Japan
    J Mol Biol 274:213-21. 1997
  2. pmc The function of the secondary DNA-binding site of RecA protein during DNA strand exchange
    A V Mazin
    Division of Biological Sciences, Section of Microbiology, University of California, Davis 95616 8665, USA
    EMBO J 17:1161-8. 1998
  3. ncbi The mutant RecA proteins, RecAR243Q and RecAK245N, exhibit defective DNA binding in homologous pairing
    H Kurumizaka
    Cellular and Molecular Biology Laboratory, The Institute of Physical and Chemical Research RIKEN, Saitama, Wako Shi, 351 0198, Japan
    Arch Biochem Biophys 365:83-91. 1999
  4. ncbi A novel, 11 nucleotide variant of chi, chi*: one of a class of sequences defining the Escherichia coli recombination hotspot chi
    D A Arnold
    Sections of Microbiology and of Molecular and Cellular Biology, University of California at Davis, CA 95616, USA
    J Mol Biol 300:469-79. 2000
  5. ncbi On the role of ATP hydrolysis in RecA protein-mediated DNA strand exchange. I. Bypassing a short heterologous insert in one DNA substrate
    J I Kim
    Department of Biochemistry, College of Agricultural and Life Sciences, University of Wisconsin, Madison 53706
    J Biol Chem 267:16438-43. 1992
  6. pmc Overproduction of the Escherichia coli recA protein without stimulation of its proteolytic activity
    B E Uhlin
    J Bacteriol 148:386-90. 1981
  7. ncbi On the role of ATP hydrolysis in RecA protein-mediated DNA strand exchange. III. Unidirectional branch migration and extensive hybrid DNA formation
    S K Jain
    Department of Biochemistry, College of Agriculture and Life Sciences, University of Wisconsin, Madison 53706
    J Biol Chem 269:20653-61. 1994
  8. ncbi Saturation mutagenesis of the E. coli RecA loop L2 homologous DNA pairing region reveals residues essential for recombination and recombinational repair
    K Hortnagel
    Genetics and Biochemistry Branch, NIDDK, National Institutes of Health, Bethesda, MD, 20892 1810, USA
    J Mol Biol 286:1097-106. 1999
  9. ncbi Recombinational DNA repair in bacteria and the RecA protein
    M M Cox
    Department of Biochemistry, University of Wisconsin Madison 53706, USA
    Prog Nucleic Acid Res Mol Biol 63:311-66. 1999
  10. pmc Two distinct modes of RecA action are required for DNA polymerase V-catalyzed translesion synthesis
    Phuong Pham
    Department of Biological Sciences, University of Southern California, Los Angeles, CA 90089 1340, USA
    Proc Natl Acad Sci U S A 99:11061-6. 2002

Research Grants

Scientific Experts

Detail Information

Publications144 found, 100 shown here

  1. ncbi An interaction between a specified surface of the C-terminal domain of RecA protein and double-stranded DNA for homologous pairing
    H Aihara
    The Institute of Physical and Chemical Research RIKEN, Wako Shi, Japan
    J Mol Biol 274:213-21. 1997
    b>RecA protein and its homologs catalyze homologous pairing of dsDNA and ssDNA, a critical reaction in homologous genetic recombination in various organisms from a virus, microbes to higher eukaryotes...
  2. pmc The function of the secondary DNA-binding site of RecA protein during DNA strand exchange
    A V Mazin
    Division of Biological Sciences, Section of Microbiology, University of California, Davis 95616 8665, USA
    EMBO J 17:1161-8. 1998
    b>RecA protein features two distinct DNA-binding sites. During DNA strand exchange, the primary site binds to single-stranded DNA (ssDNA), forming the helical RecA nucleoprotein filament...
  3. ncbi The mutant RecA proteins, RecAR243Q and RecAK245N, exhibit defective DNA binding in homologous pairing
    H Kurumizaka
    Cellular and Molecular Biology Laboratory, The Institute of Physical and Chemical Research RIKEN, Saitama, Wako Shi, 351 0198, Japan
    Arch Biochem Biophys 365:83-91. 1999
    In homologous pairing, the RecA protein sequentially binds to single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA), aligning the two DNA molecules within the helical nucleoprotein filament...
  4. ncbi A novel, 11 nucleotide variant of chi, chi*: one of a class of sequences defining the Escherichia coli recombination hotspot chi
    D A Arnold
    Sections of Microbiology and of Molecular and Cellular Biology, University of California at Davis, CA 95616, USA
    J Mol Biol 300:469-79. 2000
    ..This is manifest by both a greater modification of nuclease activity and a higher stimulation of RecA protein-mediated joint molecule formation at chi* than at chi...
  5. ncbi On the role of ATP hydrolysis in RecA protein-mediated DNA strand exchange. I. Bypassing a short heterologous insert in one DNA substrate
    J I Kim
    Department of Biochemistry, College of Agricultural and Life Sciences, University of Wisconsin, Madison 53706
    J Biol Chem 267:16438-43. 1992
    b>RecA protein promotes a substantial DNA strand exchange reaction in the presence of adenosine 5'-O-3-(thio)triphosphate (ATP gamma S) (Menetski, J.P., Bear, D.G., and Kowalczykowski, S.C. (1990) Proc. Natl. Acad. Sci. U.S.A...
  6. pmc Overproduction of the Escherichia coli recA protein without stimulation of its proteolytic activity
    B E Uhlin
    J Bacteriol 148:386-90. 1981
    Plasmid pBEU14, which carries the Escherichia coli recA+ gene and which can be amplified by manipulation of growth temperature, was constructed...
  7. ncbi On the role of ATP hydrolysis in RecA protein-mediated DNA strand exchange. III. Unidirectional branch migration and extensive hybrid DNA formation
    S K Jain
    Department of Biochemistry, College of Agriculture and Life Sciences, University of Wisconsin, Madison 53706
    J Biol Chem 269:20653-61. 1994
    We have identified two functions for RecA-mediated ATP hydrolysis during DNA strand exchange. First, ATP hydrolysis renders RecA protein-mediated DNA strand exchange unidirectional (5' to 3' with respect to the single-stranded DNA)...
  8. ncbi Saturation mutagenesis of the E. coli RecA loop L2 homologous DNA pairing region reveals residues essential for recombination and recombinational repair
    K Hortnagel
    Genetics and Biochemistry Branch, NIDDK, National Institutes of Health, Bethesda, MD, 20892 1810, USA
    J Mol Biol 286:1097-106. 1999
    The disordered mobile loop L2 of the Escherichia coli RecA protein is known to play a central role in DNA binding and pairing...
  9. ncbi Recombinational DNA repair in bacteria and the RecA protein
    M M Cox
    Department of Biochemistry, University of Wisconsin Madison 53706, USA
    Prog Nucleic Acid Res Mol Biol 63:311-66. 1999
    ..5) DNA excision repair and other repair systems eliminate lesions left behind in double-stranded DNA. The RecA protein plays a central role in the recombination phase of the process...
  10. pmc Two distinct modes of RecA action are required for DNA polymerase V-catalyzed translesion synthesis
    Phuong Pham
    Department of Biological Sciences, University of Southern California, Los Angeles, CA 90089 1340, USA
    Proc Natl Acad Sci U S A 99:11061-6. 2002
    SOS mutagenesis in Escherichia coli requires DNA polymerase V (pol V) and RecA protein to copy damaged DNA templates...
  11. ncbi Escherichia coli RecX inhibits RecA recombinase and coprotease activities in vitro and in vivo
    Elizabeth A Stohl
    Department of Microbiology and Immunology, Feinberg School of Medicine, Northwestern University, Chicago, Illinois 60611, USA
    J Biol Chem 278:2278-85. 2003
    In Escherichia coli the RecA protein plays a pivotal role in homologous recombination, DNA repair, and SOS repair and mutagenesis. A gene designated recX (or oraA) is present directly downstream of recA in E...
  12. ncbi ATP-mediated conformational changes in the RecA filament
    Margaret S VanLoock
    Department of Biochemistry and Molecular Genetics, University of Virginia Health Sciences, Charlottesville, VA 22908, USA
    Structure 11:187-96. 2003
    The crystal structure of the E. coli RecA protein was solved more than 10 years ago, but it has provided limited insight into the mechanism of homologous genetic recombination...
  13. pmc Cleavage of bacteriophage lambda cI repressor involves the RecA C-terminal domain
    Vitold E Galkin
    Department of Biochemistry and Molecular Genetics, University of Virginia, Charlottesville, VA 22908 0733, USA
    J Mol Biol 385:779-87. 2009
    ..of these genes occurs when the LexA repressor cleaves itself, a reaction catalyzed by an active, extended RecA filament formed on DNA...
  14. pmc The active form of DNA polymerase V is UmuD'(2)C-RecA-ATP
    Qingfei Jiang
    Department of Biological Sciences, University of Southern California, University Park, Los Angeles, California 90089 2910, USA
    Nature 460:359-63. 2009
    DNA-damage-induced SOS mutations arise when Escherichia coli DNA polymerase (pol) V, activated by a RecA nucleoprotein filament (RecA*), catalyses translesion DNA synthesis...
  15. ncbi Nucleotide sequence of a recA operator mutation. LexA/operator-repressor binding/inducible repair
    B E Uhlin
    Mol Gen Genet 185:251-4. 1982
  16. pmc DNA double-strand breaks caused by replication arrest
    B Michel
    Laboratoire de Genetique Microbienne, Institut National de la Recherche Agronomique, Jouy en Josas, France
    EMBO J 16:430-8. 1997
    ..In wild-type E. coli, linear DNA was detected in the absence of RecBC or of both RecA and RecD. This reveals the existence of a significant amount of spontaneous DSBs...
  17. pmc Inhibition of Escherichia coli RecA coprotease activities by DinI
    T Yasuda
    Institute for Virus Research, Kyoto University, Kyoto 606 8507, Japan
    EMBO J 17:3207-16. 1998
    ..The initial step, self-cleavage of the LexA repressor, is promoted by the RecA protein which is activated upon binding to single-stranded DNA...
  18. ncbi RuvABC-dependent double-strand breaks in dnaBts mutants require recA
    M Seigneur
    Laboratoire de Genetique Microbienne, Institut National de la Recherche Agronomique, 78352 Jouy en Josas Cedex, France
    Mol Microbiol 38:565-74. 2000
    ..that, in the rep mutant, RuvABC-mediated breakage occurs in the absence of the homologous recombination protein RecA. In contrast, in dnaBts mutants, most of the RuvABC-mediated breakage depends on the presence of RecA, which ..
  19. pmc Solution structure of DinI provides insight into its mode of RecA inactivation
    B E Ramirez
    Laboratory of Chemical Physics, National Institutes of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892 0520, USA
    Protein Sci 9:2161-9. 2000
    The Escherichia coli RecA protein triggers both DNA repair and mutagenesis in a process known as the SOS response. The 81-residue E. coli protein DinI inhibits activity of RecA in vivo...
  20. ncbi RecA protein filaments disassemble in the 5' to 3' direction on single-stranded DNA
    J M Bork
    Department of Biochemistry, University of Wisconsin, Madison, Wisconsin 53706 1544, USA
    J Biol Chem 276:45740-3. 2001
    b>RecA protein forms filaments on both single- and double-stranded DNA. Several studies confirm that filament extension occurs in the 5' to 3' direction on single-stranded DNA...
  21. ncbi DNA damage-induced replication fork regression and processing in Escherichia coli
    Justin Courcelle
    Department of Biological Sciences, Box GY, Mississippi State University, Mississippi State, MS 39762, USA
    Science 299:1064-7. 2003
    ..After ultraviolet (UV)-induced DNA damage in Escherichia coli, replication recovery requires RecA and several other recF pathway proteins...
  22. ncbi The DinI protein stabilizes RecA protein filaments
    Shelley L Lusetti
    Department of Biochemistry, University of Wisconsin Madison, Madison, Wisconsin 53706 1544, USA
    J Biol Chem 279:30037-46. 2004
    When DinI is present at concentrations that are stoichiometric with those of RecA or somewhat greater, DinI has a substantial stabilizing effect on RecA filaments bound to DNA...
  23. pmc Role of the Escherichia coli RecQ DNA helicase in SOS signaling and genome stabilization at stalled replication forks
    Takashi Hishida
    Research Institute for Microbial Diseases, Osaka University, Osaka 565 0871, Japan
    Genes Dev 18:1886-97. 2004
    ..Cells that carry dnaE486, a mutation in the DNA polymerase III alpha-catalytic subunit, induce an RecA-dependent SOS response and become highly filamented at the semirestrictive temperature (38 degrees C)...
  24. ncbi Crystal structures of Escherichia coli RecA in a compressed helical filament
    Xu Xing
    Department of Molecular and Cellular Biochemistry, Ohio State University College of Medicine and Public Health, 371 Hamilton Hall, 1645 Neil Avenue, Columbus, OH 43210, USA
    J Mol Biol 342:1471-85. 2004
    The X-ray crystal structure of uncomplexed Escherichia coli RecA protein has been determined in three new crystal forms at resolutions of 1.9 A, 2.0 A, and 2.6 A...
  25. pmc MutS inhibits RecA-mediated strand exchange with platinated DNA substrates
    Melissa A Calmann
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, 364 Plantation Street, Worcester, MA 01605, USA
    Proc Natl Acad Sci U S A 101:14174-9. 2004
    ..We used RecA-mediated strand exchange between homologous phiX174 molecules, one that was platinated and the other that was ..
  26. ncbi Measurement of SOS expression in individual Escherichia coli K-12 cells using fluorescence microscopy
    Jesse D McCool
    Department of Microbiology, Morrill Science Center IV N203, University of Massachusetts at Amherst, Amherst, MA 01003, USA
    Mol Microbiol 53:1343-57. 2004
    ..It is shown that the fluorescence in sulAp-gfp cells is regulated by RecA and LexA. This system was then used to distinguish between the two models for several mutants...
  27. pmc The RecF protein antagonizes RecX function via direct interaction
    Shelley L Lusetti
    Department of Biochemistry, University of Wisconsin, Madison, Madison, Wisconsin 53706, USA
    Mol Cell 21:41-50. 2006
    The RecX protein inhibits RecA filament extension, leading to net filament disassembly. The RecF protein physically interacts with the RecX protein and protects RecA from the inhibitory effects of RecX...
  28. pmc UvrD limits the number and intensities of RecA-green fluorescent protein structures in Escherichia coli K-12
    Richard C Centore
    Department of Microbiology, Morrill Science Center IV N203, University of Massachusetts at Amherst, Amherst, MA 01003, USA
    J Bacteriol 189:2915-20. 2007
    b>RecA is important for recombination, DNA repair, and SOS induction. In Escherichia coli, RecBCD, RecFOR, and RecJQ prepare DNA substrates onto which RecA binds...
  29. pmc New mutations in and around the L2 disordered loop of the RecA protein modulate recombination and/or coprotease activity
    F Larminat
    Laboratoire de Pharmacologie et de Toxicologie Fondamentales, Centre National de la Recherche Scientifique, Toulouse, France
    J Bacteriol 174:6264-9. 1992
    The RecA protein plays a key role in Escherichia coli recombination and DNA repair. We have created new recA mutants with mutations in the vicinity of the recA430 mutation (Gly-204----Ser) which is known to affect RecA coprotease activity...
  30. ncbi A partially deficient mutant, recA1730, that fails to form normal nucleoprotein filaments
    M Dutreix
    Groupe d Etude Mutagénèse et Cancérogénèse, Laboratoire d Enzymologie, CNRS, Gif sur Yvette, France
    Mol Gen Genet 232:489-97. 1992
    ..ssDNA equally well on nitrocellulose filters; this activity was specifically inhibited by a monoclonal anti-RecA antibody...
  31. ncbi The structure of the E. coli recA protein monomer and polymer
    R M Story
    Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06511
    Nature 355:318-25. 1992
    The crystal structure of the recA protein from Escherichia coli at 2.3-A resolution reveals a major domain that binds ADP and probably single- and double-stranded DNA...
  32. ncbi Structure of the recA protein-ADP complex
    R M Story
    Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06511
    Nature 355:374-6. 1992
    The recA protein catalyses the ATP-driven homologous pairing and strand exchange of DNA molecules...
  33. pmc New recA mutations that dissociate the various RecA protein activities in Escherichia coli provide evidence for an additional role for RecA protein in UV mutagenesis
    M Dutreix
    Groupe d Etude Mutagénèse et Cancérogénèse, Enzymologie, Centre National de la Recherche Scientifique, Gif sur Yvette, France
    J Bacteriol 171:2415-23. 1989
    To isolate strains with new recA mutations that differentially affect RecA protein functions, we mutagenized in vitro the recA gene carried by plasmid mini-F and then introduced the mini-F-recA plasmid into a delta recA host that was ..
  34. ncbi General mechanism for RecA protein binding to duplex DNA
    B F Pugh
    Department of Biochemistry, College of Agriculture and Life Sciences, University of Wisconsin Madison 53706
    J Mol Biol 203:479-93. 1988
    b>RecA protein binding to duplex DNA occurs by a multi-step process...
  35. pmc Characteristics of some multiply recombination-deficient strains of Escherichia coli
    N S Willetts
    J Bacteriol 100:231-9. 1969
    ..The following genotypes were constructed and verified: recC22 recB21 recA(+), recC22 recB21 recA13, recC22 recB(+)recA13, and recC(+)recB21 recA13...
  36. pmc Genetic analysis of the RecE pathway of genetic recombination in Escherichia coli K-12
    J R Gillen
    J Bacteriol 145:521-32. 1981
    ..In addition, strain construction and mutagenesis revealed the dependence of the RecE pathway on recA+ and on recF+. Dependence on a fourth gene was shown by a mutation (rec-77) which does not map near the other genes...
  37. ncbi Functional domains of Escherichia coli recA protein deduced from the mutational sites in the gene
    H Kawashima
    Mol Gen Genet 193:288-92. 1984
    The sites of recA mutations of Escherichia coli, recA441 (tif-1), recA1, recA430 (lexB30) and recA44, were determined by analyses of the nucleotide sequences...
  38. pmc Biochemistry of homologous recombination in Escherichia coli
    S C Kowalczykowski
    Division of Biological Sciences, University of California, Davis 95616 8665
    Microbiol Rev 58:401-65. 1994
    ..At least 25 gene products are involved in promoting genetic exchange. At present, this includes the RecA, RecBCD (exonuclease V), RecE (exonuclease VIII), RecF, RecG, RecJ, RecN, RecOR, RecQ, RecT, RuvAB, RuvC, SbcCD, ..
  39. pmc Biochemical interaction of the Escherichia coli RecF, RecO, and RecR proteins with RecA protein and single-stranded DNA binding protein
    K Umezu
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115
    Proc Natl Acad Sci U S A 90:3875-9. 1993
    The Escherichia coli RecF, RecO, and RecR proteins were analyzed for their effect on RecA-mediated pairing of single-stranded circular DNA and homologous linear duplex DNA substrates...
  40. ncbi RecA protein filaments: end-dependent dissociation from ssDNA and stabilization by RecO and RecR proteins
    Q Shan
    Department of Biochemistry, University of Wisconsin Madison 53706, USA
    J Mol Biol 265:519-40. 1997
    b>RecA protein filaments formed on circular (ssDNA) in the presence of ssDNA binding protein (SSB) are generally stable as long as ATP is regenerated...
  41. pmc recF and recR are required for the resumption of replication at DNA replication forks in Escherichia coli
    J Courcelle
    Department of Biological Sciences, Stanford University, CA 94305 5020, USA
    Proc Natl Acad Sci U S A 94:3714-9. 1997
    ..Current biochemical and genetic data on the conditions under which recF-mediated recombination occurs suggest that the recombinational intermediate also may mimic the structure of a disrupted replication fork...
  42. ncbi RecA protein: structure, function, and role in recombinational DNA repair
    A I Roca
    Department of Biochemistry, College of Agriculture and Life Sciences, University of Wisconsin, Madison 53706, USA
    Prog Nucleic Acid Res Mol Biol 56:129-223. 1997
  43. ncbi DNA strand exchange proteins: a biochemical and physical comparison
    P R Bianco
    Section of Microbiology and of Molecular and Cellular Biology, USC School of Medicine, 1441 Eastlake Ave, Los Angeles, CA 90033, USA
    Front Biosci 3:D570-603. 1998
    ..In Escherichia coli, the RecA protein is essential to recombination, and biochemical analysis demonstrates that it is responsible for the crucial ..
  44. ncbi Essential monomer-monomer contacts define the minimal length for the N-terminus of RecA protein
    E N Zaitsev
    Mol Microbiol 29:1317-8. 1998
  45. pmc Recombinational repair of DNA damage in Escherichia coli and bacteriophage lambda
    A Kuzminov
    Institute of Molecular Biology, University of Oregon, Eugene, Oregon 97403, USA
    Microbiol Mol Biol Rev 63:751-813, table of contents. 1999
    ..Putting our knowledge about recombinational repair in the broader context of DNA replication will guide future experimentation...
  46. ncbi Specific amino acid changes enhance the anti-recombination activity of the UmuD'C complex
    S Sommer
    Institut Curie, Bat 110, Centre Universitaire, F 91405 Orsay, France
    Mol Microbiol 35:1443-53. 2000
    ..the UmuD'C complex sensitizes recA+ bacteria to DNA damage, whereas it has no effect on bacteria expressing a RecA [UmuR] protein that overcomes recombination inhibition...
  47. ncbi Identification of additional genes belonging to the LexA regulon in Escherichia coli
    A R Fernandez de Henestrosa
    Section on DNA Replication, Repair and Mutagenesis, Building 6, Room 1A13, National Institute of Child Health and Human Development, National Institutes of Health, 9000 Rockville Pike, Bethesda, MD 20892 2725, USA
    Mol Microbiol 35:1560-72. 2000
    ..These experiments have allowed us to identify seven new LexA-regulated genes, thus bringing the present number of genes in the E. coli LexA regulon to 31. The potential function of each newly identified LexA-regulated gene is discussed...
  48. pmc Physical interactions between DinI and RecA nucleoprotein filament for the regulation of SOS mutagenesis
    T Yasuda
    Institute for Virus Research, Kyoto University, Japan
    EMBO J 20:1192-202. 2001
    ..and resulted in the inhibition of LexA and UmuD processing, reactions that are normally dependent on activated RecA in a complex with single-stranded (ss)DNA...
  49. pmc RecA protein promotes the regression of stalled replication forks in vitro
    M E Robu
    Department of Biochemistry, University of Wisconsin, Madison, WI 53706 1544, USA
    Proc Natl Acad Sci U S A 98:8211-8. 2001
    ..designed to mimic one of the known structures of a fork stalled at a leading-strand lesion, we show here that RecA protein of Escherichia coli will promote a fork regression reaction in vitro...
  50. pmc The RecOR proteins modulate RecA protein function at 5' ends of single-stranded DNA
    J M Bork
    Department of Biochemistry, University of Wisconsin Madison, Madison, WI 53706 1544, USA
    EMBO J 20:7313-22. 2001
    ..The RecOR-facilitated binding of RecA protein to single-stranded DNA (ssDNA) that is bound by single-stranded DNA-binding protein (SSB) is much faster if ..
  51. ncbi Replication restart in UV-irradiated Escherichia coli involving pols II, III, V, PriA, RecA and RecFOR proteins
    Savithri Rangarajan
    Department of Biological Sciences and Chemistry, Hedco Molecular Biology Laboratories, University of Southern California, Los Angeles, CA 90089 1340, USA
    Mol Microbiol 43:617-28. 2002
    ..we have analysed the time dependence of replication restart in strains carrying a combination of mutations in lexA, recA, polB (pol II), umuDC (pol V), priA, dnaC, recF, recO or recR...
  52. ncbi The bacterial RecA protein and the recombinational DNA repair of stalled replication forks
    Shelley L Lusetti
    Department of Biochemistry, University of Wisconsin Madison, 433 Babcock Drive, Madison, Wisconsin 53706 1544, USA
    Annu Rev Biochem 71:71-100. 2002
    ..The RecA protein plays a central role in these repair pathways, and its biochemistry must be considered in this context...
  53. pmc RecX protein abrogates ATP hydrolysis and strand exchange promoted by RecA: insights into negative regulation of homologous recombination
    R Venkatesh
    Department of Biochemistry, Indian Institute of Science, Bangalore 560012, India
    Proc Natl Acad Sci U S A 99:12091-6. 2002
    In many eubacteria, coexpression of recX with recA is essential for attenuation of the deleterious effects of recA overexpression; however, the molecular mechanism has remained enigmatic...
  54. ncbi recX, a new SOS gene that is co-transcribed with the recA gene in Escherichia coli
    Vincent Pages
    UPR9003, CNRS Cancerogenese et Mutagenese Moleculaire et Structurale, ESBS, Blvd Sebastien Brant, 67400, Strasbourg, France
    DNA Repair (Amst) 2:273-84. 2003
    recX is a small open reading frame located downstream of recA that is conserved in many bacteria. In Escherichia coli, the recX gene (also named oraA) is a 501 bp open reading frame that encodes a predicted basic protein...
  55. ncbi Magnesium ion-dependent activation of the RecA protein involves the C terminus
    Shelley L Lusetti
    Department of Biochemistry, University of Wisconsin, 433 Babcock Drive, Madison, WI 53706, USA
    J Biol Chem 278:16381-8. 2003
    Optimal conditions for RecA protein-mediated DNA strand exchange include 6-8 mm Mg(2+) in excess of that required to form complexes with ATP...
  56. pmc An NMR study on the interaction of Escherichia coli DinI with RecA-ssDNA complexes
    Masatoshi Yoshimasu
    Cellular and Molecular Biology Laboratory, RIKEN, 2 1 Hirosawa, Wako Shi, Saitama 351 0198, Japan
    Nucleic Acids Res 31:1735-43. 2003
    ..that include DNA damage-induced replication arrest, and is positively regulated by the co- protease activity of RecA. Escherichia coli DinI, a LexA-regulated SOS gene product, shuts off the initiation of the SOS response when ..
  57. ncbi Biochemical characterization of a mutant RecA protein altered in DNA-binding loop 1
    Julie K Mirshad
    Department of Chemistry, University of California, Davis, California 95616, USA
    Biochemistry 42:5945-54. 2003
    The double substitution of Glu156 with Leu and Gly157 with Val in the Escherichia coli RecA protein results in a severely reduced level of recombination and constitutive coprotease behavior...
  58. ncbi RecFOR proteins load RecA protein onto gapped DNA to accelerate DNA strand exchange: a universal step of recombinational repair
    Katsumi Morimatsu
    Section of Microbiology, Center for Genetics and Development, University of California, Davis, 95616, USA
    Mol Cell 11:1337-47. 2003
    ..Here, we show that the concerted action of the RecFOR complex directs the loading of RecA protein specifically onto gapped DNA that is coated with single-stranded DNA binding (SSB) protein, thereby ..
  59. ncbi The bacterial RecA protein as a motor protein
    Michael M Cox
    Department of Biochemistry, University of Wisconsin Madison, Madison, Wisconsin 53706 1544, USA
    Annu Rev Microbiol 57:551-77. 2003
    The bacterial RecA protein plays a central role in the repair of stalled replication forks, double-strand break repair, general recombination, induction of the SOS response, and SOS mutagenesis...
  60. ncbi RecA-dependent recovery of arrested DNA replication forks
    Justin Courcelle
    Department of Biological Sciences, Box GY, Mississippi State University, Mississippi State, Mississippi 39762, USA
    Annu Rev Genet 37:611-46. 2003
    ..The RecA protein of Escherichia coli is essential for survival in this situation: It maintains the integrity of the arrested ..
  61. ncbi Molecular design and functional organization of the RecA protein
    Dharia A McGrew
    Department of Biochemistry and Molecular Pharmacology, Aaron Lazare Research Building, University of Massachusetts Medical School, 364 Plantation Street, Worcester, MA 01605 2324, USA
    Crit Rev Biochem Mol Biol 38:385-432. 2003
    The bacterial RecA protein participates in a remarkably diverse set of functions, all of which are involved in the maintenance of genomic integrity...
  62. ncbi RecG helicase promotes DNA double-strand break repair
    Tom R Meddows
    Institute of Genetics, University of Nottingham, Queen s Medical Centre, Nottingham NG7 2UH, UK
    Mol Microbiol 52:119-32. 2004
    ..In Escherichia coli, repair relies on the RecBCD and RecA proteins, the combined ability of which to initiate recombination and form joint-molecule intermediates is well ..
  63. ncbi What is the structure of the RecA-DNA filament?
    X Yu
    Department of Biochemistry, University of Virginia Health Sciences Center, Charlottesville VA 22908 0733, USA
    Curr Protein Pept Sci 5:73-9. 2004
    The bacterial RecA protein has been a model system for understanding how a protein can catalyze homologous genetic recombination. RecA-like proteins have now been characterized from many organisms, from bacteriophage to humans...
  64. ncbi DNA double-strand breaks induce deletion of CTG.CAG repeats in an orientation-dependent manner in Escherichia coli
    Micheal L Hebert
    Institute of Biosciences and Technology, Center for Genome Research, Texas A and M University System Health Science Center, Texas Medical Center, 2121 W Holcombe Blavd, Houston, TX 77030 3303, USA
    J Mol Biol 336:655-72. 2004
    ..CCG repeats were linearized in vitro near the center of the repeats and were transformed into parental, RecA-dependent homologous recombination-deficient, or RecBC exonuclease-deficient Escherichia coli...
  65. ncbi Spontaneous and cisplatin-induced recombination in Escherichia coli
    Anetta Nowosielska
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, 364 Plantation Street, LRB823 Worcester, MA 01655, USA
    DNA Repair (Amst) 3:719-28. 2004
    ..Lac+ formation for both spontaneous and cisplatin-induced recombination requires the products of the recA, recBC, ruvA, ruvB, ruvC, priA and polA genes...
  66. ncbi The biochemical requirements of DNA polymerase V-mediated translesion synthesis revisited
    Shingo Fujii
    UPR 9003 du CNRS, Cancerogenese et Mutagenese Moleculaire et Structurale, 67400 Strasbourg, France
    J Mol Biol 341:405-17. 2004
    ..In addition, RecA protein and the replication processivity factor, the beta-clamp, were genetically found to be essential co-factors ..
  67. ncbi A RecA filament capping mechanism for RecX protein
    Julia C Drees
    Department of Biochemistry, University of Wisconsin Madison, Madison, WI 53706, USA
    Mol Cell 15:789-98. 2004
    The RecX protein is a potent inhibitor of RecA protein activities. RecX functions by specifically blocking the extension of RecA filaments. In vitro, this leads to a net disassembly of RecA protein from circular single-stranded DNA...
  68. ncbi Genetic recombination destabilizes (CTG)n.(CAG)n repeats in E. coli
    Vera I Hashem
    Laboratory of DNA Structure and Mutagenesis, Center for Genome Research, Institute of Biosciences and Technology, Texas A and M University, 2121 West Holcombe Blvd, Houston 77030 3303, USA
    Mutat Res 554:95-109. 2004
    ..Using a genetic assay for repeat deletion in E. coli, the effect of mutations in the recA, recB, and lexA genes on the rate of deletion of (CTG)n.(CAG)n repeats of varying lengths were examined...
  69. ncbi Inhibition of RecA protein by the Escherichia coli RecX protein: modulation by the RecA C terminus and filament functional state
    Julia C Drees
    Department of Biochemistry, University of Wisconsin Madison, 433 Babcock Dr, Madison, WI 53706 1544, USA
    J Biol Chem 279:52991-7. 2004
    The RecX protein is a potent inhibitor of RecA activities. We identified several factors that affect RecX-RecA interaction. The interaction is enhanced by the RecA C terminus and by significant concentrations of free Mg(2+) ion...
  70. pmc UvrD helicase, unlike Rep helicase, dismantles RecA nucleoprotein filaments in Escherichia coli
    Xavier Veaute
    CEA, DSV, DRR, UMR217 CNRS CEA, Fontenay aux Roses, France
    EMBO J 24:180-9. 2005
    ..In addition to RecFOR, we provide evidence that RecA contributes to toxicity in the rep uvrD mutant...
  71. ncbi Cellular response to horizontally transferred DNA in Escherichia coli is tuned by DNA repair systems
    Stéphane Delmas
    INSERM U571, Faculté de Médecine Necker Enfants Malades Université Paris V, 156 rue Vaugirard, 75730 Paris Cedex 15, France
    DNA Repair (Amst) 4:221-9. 2005
    ..and frequency of recombination, suggest that the level of SOS induction precisely reflects the difficulty of RecA protein to initiate a productive strand exchange process...
  72. ncbi Crystal structures of Escherichia coli RecA in complex with MgADP and MnAMP-PNP
    Xu Xing
    Department of Molecular and Cellular Biochemistry, Ohio State University College of Medicine and Public Health, 371 Hamilton Hall, 1645 Neil Avenue, Columbus, Ohio 43210, USA
    Biochemistry 43:16142-52. 2004
    b>RecA catalyzes the DNA pairing and strand-exchange steps of homologous recombination, an important mechanism for repair of double-stranded DNA breaks. The binding of RecA to DNA is modulated by adenosine nucleotides...
  73. ncbi DNA polymerase V and RecA protein, a minimal mutasome
    Katharina Schlacher
    Department of Biological Sciences, University of Southern California, Los Angeles, CA 90089, USA
    Mol Cell 17:561-72. 2005
    ..TLS requires DNA polymerase V (UmuD'2C) and RecA. Here, we show that pol V and RecA interact by two distinct mechanisms...
  74. pmc Roles of the Escherichia coli RecA protein and the global SOS response in effecting DNA polymerase selection in vivo
    Robert W Maul
    Department of Biochemistry, School of Medicine and Biomedical Sciences, State University of New York at Buffalo, 14214, USA
    J Bacteriol 187:7607-18. 2005
    ..In contrast, chronically activated RecA protein, expressed from the recA730 allele, is lethal to the dnaN159 strain, and this lethality is suppressed by ..
  75. ncbi Inhibition of RecA protein function by the RdgC protein from Escherichia coli
    Julia C Drees
    Department of Biochemistry, University of Wisconsin, Madison, Wisconsin 53706 1544, USA
    J Biol Chem 281:4708-17. 2006
    The Escherichia coli RdgC protein is a potential negative regulator of RecA function. RdgC inhibits RecA protein-promoted DNA strand exchange, ATPase activity, and RecA-dependent LexA cleavage...
  76. ncbi RecA acts in trans to allow replication of damaged DNA by DNA polymerase V
    Katharina Schlacher
    Department of Biological Sciences and Chemistry, University of Southern California, University Park, Los Angeles, California 90089 2910, USA
    Nature 442:883-7. 2006
    The DNA polymerase V (pol V) and RecA proteins are essential components of a mutagenic translesion synthesis pathway in Escherichia coli designed to cope with DNA damage...
  77. ncbi Direct observation of individual RecA filaments assembling on single DNA molecules
    Roberto Galletto
    Section of Microbiology, Center for Genetics and Development, Davis, California 95616, USA
    Nature 443:875-8. 2006
    Escherichia coli RecA is essential for the repair of DNA double-strand breaks by homologous recombination. Repair requires the formation of a RecA nucleoprotein filament...
  78. pmc RecA-independent recombination is efficient but limited by exonucleases
    Bethany E Dutra
    Rosenstiel Basic Medical Sciences Research Center and Department of Biology, Brandeis University, Waltham, MA 02454 9110, USA
    Proc Natl Acad Sci U S A 104:216-21. 2007
    Genetic recombination in bacteria is facilitated by the RecA strand transfer protein and strongly depends on the homology between interacting sequences...
  79. pmc Spontaneous DNA breakage in single living Escherichia coli cells
    Jeanine M Pennington
    Interdepartmental Program in Cell and Molecular Biology and Department of Molecular and Human Genetics, Houston, Texas 77030 3411, USA
    Nat Genet 39:797-802. 2007
    ..Finally, FACS demonstrated two main cell fates after spontaneous DNA damage: viability with or without resumption of proliferation...
  80. ncbi XthA (Exonuclease III) regulates loading of RecA onto DNA substrates in log phase Escherichia coli cells
    Richard C Centore
    Molecular and Cellular Biology Graduate Program, Morrill Science Center, University of Massachusetts at Amherst, Amherst, MA 01003, USA
    Mol Microbiol 67:88-101. 2008
    ..In Escherichia coli, the effects of several 3'-5' exonucleases on RecA loading were studied by assaying RecA-GFP foci formation...
  81. pmc UmuD and RecA directly modulate the mutagenic potential of the Y family DNA polymerase DinB
    Veronica G Godoy
    Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Mol Cell 28:1058-70. 2007
    ..We show that the DNA damage-inducible proteins UmuD(2) and RecA act in concert to modulate this mutagenic activity...
  82. doi Mechanism of homologous recombination from the RecA-ssDNA/dsDNA structures
    Zhucheng Chen
    Structural Biology Program, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    Nature 453:489-4. 2008
    The RecA family of ATPases mediates homologous recombination, a reaction essential for maintaining genomic integrity and for generating genetic diversity...
  83. pmc Structural basis for inhibition of homologous recombination by the RecX protein
    Stefania Ragone
    Department of Biochemistry, University of Cambridge, Cambridge, UK
    EMBO J 27:2259-69. 2008
    The RecA/RAD51 nucleoprotein filament is central to the reaction of homologous recombination (HR). Filament activity must be tightly regulated in vivo as unrestrained HR can cause genomic instability...
  84. pmc Reconstitution of initial steps of dsDNA break repair by the RecF pathway of E. coli
    Naofumi Handa
    Department of Microbiology, University of California at Davis, Davis, Calfironia 95616, USA
    Genes Dev 23:1234-45. 2009
    ..reconstitute in vitro a seven-protein reaction that recapitulates early steps of dsDNA break repair using purified RecA, RecF, RecO, RecR, RecQ, RecJ, and SSB proteins, components of the RecF system...
  85. pmc Directed evolution of ionizing radiation resistance in Escherichia coli
    Dennis R Harris
    Department of Biochemistry, University of Wisconsin Madison, 433 Babcock Drive, Madison, WI 53575, USA
    J Bacteriol 191:5240-52. 2009
    ..Several mutations in the recA gene and a deletion of the e14 prophage both demonstrably contribute to and partially explain the new phenotype...
  86. pmc SSB protein diffusion on single-stranded DNA stimulates RecA filament formation
    Rahul Roy
    Center for Biophysics and Computational Biology, University of Illinois, Urbana Champaign, Illinois 61801, USA
    Nature 461:1092-7. 2009
    ..Diffusional migration of SSB helps in the local displacement of SSB by an elongating RecA filament...
  87. pmc Two modes of binding of DinI to RecA filament provide a new insight into the regulation of SOS response by DinI protein
    Vitold E Galkin
    Department of Biochemistry and Molecular Genetics, University of Virginia, Jordan Hall 6007, 1340 Jefferson Park Avenue, Charlottesville, VA 22908, USA
    J Mol Biol 408:815-24. 2011
    b>RecA protein plays a principal role in bacterial SOS response to DNA damage. The induction of the SOS response is well understood and involves the cleavage of the LexA repressor catalyzed by the RecA nucleoprotein filament...
  88. ncbi Assembly and disassembly of RecA protein filaments occur at opposite filament ends. Relationship to DNA strand exchange
    J E Lindsley
    Department of Biochemistry, College of Agricultural and Life Sciences, University of Wisconsin, Madison 53706
    J Biol Chem 265:9043-54. 1990
    b>RecA protein primarily associates with and dissociates from opposite ends of nucleoprotein filaments formed on linear duplex DNA...
  89. pmc Genetic location of certain mutations conferring recombination deficiency in Escherichia coli
    N S Willetts
    J Bacteriol 97:244-9. 1969
    ..All four mutations map at similar distances from cysC and pheA and, despite the different degrees of apparent dominance, all may lie in the recA gene.
  90. ncbi A comparative study of global stress gene regulation in response to overexpression of recombinant proteins in Escherichia coli
    R T Gill
    Department of Chemical Engineering, University of Maryland, College Park, Maryland 20742, USA
    Metab Eng 2:178-89. 2000
    ..increased upregulation of heat shock (ftsH, clpP, lon, ompT, degP, groEL, aceA, ibpA), SOS/DNA damage (recA, lon, IS5 transposase), stationary phase (rpoS, aceA), and bacteriophage life cycle (ftsH, recA) genes...
  91. ncbi The DinI and RecX proteins are competing modulators of RecA function
    Shelley L Lusetti
    Department of Biochemistry, University of Wisconsin Madison, 433 Babcock Drive, Madison, WI 53706 1544, USA
    J Biol Chem 279:55073-9. 2004
    The DinI and RecX proteins of Escherichia coli both modulate the function of RecA protein, but have very different effects. DinI protein stabilizes RecA filaments, preventing disassembly but permitting assembly...
  92. ncbi Localization of RecA in Escherichia coli K-12 using RecA-GFP
    Nicholas Renzette
    Molecular and Cellular Biology Graduate Program, University of Massachusetts, Amherst, MA 01003, USA
    Mol Microbiol 57:1074-85. 2005
    b>RecA is important in recombination, DNA repair and repair of replication forks. It functions through the production of a protein-DNA filament...
  93. pmc RecA-mediated SOS induction requires an extended filament conformation but no ATP hydrolysis
    Marielle C Gruenig
    Department of Biochemistry, 433 Babcock Drive, University of Wisconsin, Madison, WI 53706, USA
    Mol Microbiol 69:1165-79. 2008
    The Escherichia coli SOS response to DNA damage is modulated by the RecA protein, a recombinase that forms an extended filament on single-stranded DNA and hydrolyzes ATP...
  94. ncbi Three-strand exchange by the Escherichia coli RecA protein using ITP as a nucleotide cofactor: mechanistic parallels with the ATP-dependent reaction of the RecA protein from Streptococcus pneumoniae
    Francine S Katz
    Department of Biochemistry, The Johns Hopkins University, Bloomberg School of Public Health, Baltimore, Maryland 21205
    J Biol Chem 278:35889-96. 2003
    The RecA protein from Escherichia coli promotes an ATP-dependent three-strand exchange reaction between a circular single-stranded DNA (ssDNA) and a homologous linear double-stranded (dsDNA)...
  95. pmc The RecB nuclease domain binds to RecA-DNA filaments: implications for filament loading
    Debora Lucarelli
    Cancer Research UK Clare Hall Laboratories, The London Research Institute, Herts, U K
    J Mol Biol 391:269-74. 2009
    The E. coli RecBCD enzyme facilitates the loading of RecA onto single-stranded DNA produced by the combined helicase/nuclease activity of RecBCD. The nuclease domain of RecB protein, RecB(nuc), has been previously shown to bind RecA...
  96. ncbi Enhancement of recA protein-promoted DNA strand exchange activity by volume-occupying agents
    P E Lavery
    Department of Cell, Molecular, and Structural Biology, Northwestern University Medical School, Chicago, Illinois 60611
    J Biol Chem 267:9307-14. 1992
    ..the effect of inert macromolecules such as polyvinyl alcohol and polyethylene glycol on the in vitro activity of recA protein...
  97. pmc Unusual stability of recombination intermediates made by Escherichia coli RecA protein
    B Muller
    Imperial Cancer Research Fund, Clare Hall Laboratories, South Mimms, Herts, London, UK
    EMBO J 11:2685-93. 1992
    The structure and stability of recombination intermediates made by RecA protein have been investigated following deproteinization...
  98. pmc The synapsis event in the homologous pairing of DNAs: RecA recognizes and pairs less than one helical repeat of DNA
    P Hsieh
    Genetics and Biochemistry Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892
    Proc Natl Acad Sci U S A 89:6492-6. 1992
    A key step in homologous recombination is the alignment and pairing of homologous DNAs. The Escherichia coli RecA protein initiates pairing by binding to single-strand DNA, forming a helical nucleoprotein filament...
  99. pmc Constitutive and UV-mediated activation of RecA protein: combined effects of recA441 and recF143 mutations and of addition of nucleosides and adenine
    M Sassanfar
    Section of Biochemistry, Molecular and Cell Biology, Cornell University, Ithaca, New York 14853
    J Bacteriol 173:5869-75. 1991
    The recF143 mutant of Escherichia coli is deficient in certain functions that also require the RecA protein: cell survival after DNA damage, some pathways of genetic recombination, and induction of SOS genes and temperate bacteriophage ..
  100. ncbi Inhibition of recA protein promoted ATP hydrolysis. 1. ATP gamma S and ADP are antagonistic inhibitors
    J W Lee
    Department of Biochemistry, College of Agricultural and Life Sciences, University of Wisconsin Madison 53706
    Biochemistry 29:7666-76. 1990
    ADP and adenosine 5'-O-(3-thiotriphosphate) (ATP gamma S) inhibit recA protein promoted ATP hydrolysis by fundamentally different mechanisms. In both cases, at least two modes of inhibition are observed...

Research Grants3

  1. Purchase of Transmission Electron Microscope (TEM)
    Michael Cox; Fiscal Year: 2003
    ..In addition, its operation will be integrated with that of the two other electron microscopes on campus (of more recent vintage), which are located in the veterinary and medical schools. ..
  2. Bacterial Proteins Involved in DNA Repair
    Michael Cox; Fiscal Year: 2007
    ..The work is currently focused on the processes that regulate the function of the RecA protein. The first specific aim constitutes the major portion of the effort...
  3. Double strand DNA break repair in D. radiodurans
    Michael Cox; Fiscal Year: 2008
    ..Nascent work on a few Deinococcus proteins, such as the Dr RecA protein, have already generated surprises that speak to the potential of an investigation of Deinococcus DNA repair ..