phoR

Summary

Gene Symbol: phoR
Description: sensory histidine kinase in two-component regulatory system with PhoB
Alias: ECK0394, JW0390, R1pho, nmpB, phoR1
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. ncbi Regulation of the phosphate regulon of Escherichia coli: properties of phoR deletion mutants and subcellular localization of PhoR protein
    M Yamada
    Department of Experimental Chemotherapy, Osaka University, Japan
    Mol Gen Genet 220:366-72. 1990
  2. ncbi Topology of the PhoR protein of Escherichia coli and functional analysis of internal deletion mutants
    M Scholten
    Department of Molecular Cell Biology, University of Utrecht, The Netherlands
    Mol Microbiol 8:269-75. 1993
  3. pmc The PhoU protein from Escherichia coli interacts with PhoR, PstB, and metals to form a phosphate-signaling complex at the membrane
    Stewart G Gardner
    Department of Microbiology and Molecular Biology, Brigham Young University, Provo, Utah, USA
    J Bacteriol 196:1741-52. 2014
  4. pmc Helix bundle loops determine whether histidine kinases autophosphorylate in cis or in trans
    Orr Ashenberg
    Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    J Mol Biol 425:1198-209. 2013
  5. doi The phosphotransferase protein EIIA(Ntr) modulates the phosphate starvation response through interaction with histidine kinase PhoR in Escherichia coli
    Denise Lüttmann
    Department of General Microbiology, Institute of Microbiology and Genetics, Georg August University, Grisebachstrasse 8, 37077 Gottingen, Germany
    Mol Microbiol 86:96-110. 2012
  6. ncbi Transcriptome analysis of phosphate starvation response in Escherichia coli
    Jong Hwan Baek
    Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering BK21 Program and BioProcess Engineering Research Center, Korea Advanced Institute of Science and Technology, Daejeon 305 701, Korea
    J Microbiol Biotechnol 17:244-52. 2007
  7. ncbi Transcript and protein level analyses of the interactions among PhoB, PhoR, PhoU and CreC in response to phosphate starvation in Escherichia coli
    Jong Hwan Baek
    FEMS Microbiol Lett 277:254-9. 2007
  8. pmc Constitutive activation of the Escherichia coli Pho regulon upregulates rpoS translation in an Hfq-dependent fashion
    Natividad Ruiz
    Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544, USA
    J Bacteriol 185:5984-92. 2003
  9. pmc Genetic and biochemical studies of phosphatase activity of PhoR
    Daniel O Carmany
    Microbiology and Molecular Biology Department, Brigham Young University, Provo, UT 84602 5253, USA
    J Bacteriol 185:1112-5. 2003
  10. ncbi Investigation of in vivo cross-talk between key two-component systems of Escherichia coli
    Daniel T Verhamme
    Swammerdam Institute for Life Sciences, University of Amsterdam, Nieuwe Achtergracht 166, 1018 WV Amsterdam, The Netherlands
    Microbiology 148:69-78. 2002

Detail Information

Publications24

  1. ncbi Regulation of the phosphate regulon of Escherichia coli: properties of phoR deletion mutants and subcellular localization of PhoR protein
    M Yamada
    Department of Experimental Chemotherapy, Osaka University, Japan
    Mol Gen Genet 220:366-72. 1990
    The phoR gene is a bifunctional regulatory gene for the phosphate regulon of Escherichia coli...
  2. ncbi Topology of the PhoR protein of Escherichia coli and functional analysis of internal deletion mutants
    M Scholten
    Department of Molecular Cell Biology, University of Utrecht, The Netherlands
    Mol Microbiol 8:269-75. 1993
    The PhoR protein of Escherichia coli K-12 belongs to a family of structurally related sensor-kinases that regulate responses to environmental stimuli...
  3. pmc The PhoU protein from Escherichia coli interacts with PhoR, PstB, and metals to form a phosphate-signaling complex at the membrane
    Stewart G Gardner
    Department of Microbiology and Molecular Biology, Brigham Young University, Provo, Utah, USA
    J Bacteriol 196:1741-52. 2014
    ..along with the high-affinity, Pi-specific ATP-binding cassette transporter PstSCAB and the two-component proteins PhoR and PhoB, is absolutely required for Pi signaling in Escherichia coli...
  4. pmc Helix bundle loops determine whether histidine kinases autophosphorylate in cis or in trans
    Orr Ashenberg
    Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    J Mol Biol 425:1198-209. 2013
    ..model by replacing the loop from Escherichia coli EnvZ, which autophosphorylates in trans, with the loop from three PhoR orthologs that autophosphorylate in cis...
  5. doi The phosphotransferase protein EIIA(Ntr) modulates the phosphate starvation response through interaction with histidine kinase PhoR in Escherichia coli
    Denise Lüttmann
    Department of General Microbiology, Institute of Microbiology and Genetics, Georg August University, Grisebachstrasse 8, 37077 Gottingen, Germany
    Mol Microbiol 86:96-110. 2012
    ..The pho regulon, which comprises more than 30 genes, is activated by the two-component system PhoR/PhoB under conditions of phosphate starvation...
  6. ncbi Transcriptome analysis of phosphate starvation response in Escherichia coli
    Jong Hwan Baek
    Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering BK21 Program and BioProcess Engineering Research Center, Korea Advanced Institute of Science and Technology, Daejeon 305 701, Korea
    J Microbiol Biotechnol 17:244-52. 2007
    Escherichia coli has a PhoR-PhoB two-component regulatory system to detect and respond to the changes of environmental phosphate concentration. For the E...
  7. ncbi Transcript and protein level analyses of the interactions among PhoB, PhoR, PhoU and CreC in response to phosphate starvation in Escherichia coli
    Jong Hwan Baek
    FEMS Microbiol Lett 277:254-9. 2007
    The responses to phosphate starvation by PhoR-PhoB two-component regulatory system are also affected by other regulatory systems in Escherichia coli...
  8. pmc Constitutive activation of the Escherichia coli Pho regulon upregulates rpoS translation in an Hfq-dependent fashion
    Natividad Ruiz
    Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544, USA
    J Bacteriol 185:5984-92. 2003
    ..This upregulation of rpoS translation is Hfq dependent, suggesting the involvement of a small regulatory RNA (sRNA). The transcription of this yet-to-be-identified sRNA is regulated by the PhoBR two-component system...
  9. pmc Genetic and biochemical studies of phosphatase activity of PhoR
    Daniel O Carmany
    Microbiology and Molecular Biology Department, Brigham Young University, Provo, UT 84602 5253, USA
    J Bacteriol 185:1112-5. 2003
    In Escherichia coli, PhoR is the histidine kinase of the phosphate regulon. It has been postulated that PhoR may function as a phospho-PhoB phosphatase...
  10. ncbi Investigation of in vivo cross-talk between key two-component systems of Escherichia coli
    Daniel T Verhamme
    Swammerdam Institute for Life Sciences, University of Amsterdam, Nieuwe Achtergracht 166, 1018 WV Amsterdam, The Netherlands
    Microbiology 148:69-78. 2002
    ..reporting Arc activation), upon activation of the Pho system, appeared to be independent of signalling through PhoR. Cross-talk towards NtrC did occur, however, in a ntrB deletion strain, upon joint activation of Pho, Ntr and Uhp...
  11. pmc Autoamplification of a two-component regulatory system results in "learning" behavior
    S M Hoffer
    Department of Molecular Microbiology and Institute for Biomembranes, Utrecht University, 3584 CH Utrecht, The Netherlands
    J Bacteriol 183:4914-7. 2001
    ....
  12. pmc Use of new methods for construction of tightly regulated arabinose and rhamnose promoter fusions in studies of the Escherichia coli phosphate regulon
    A Haldimann
    Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907, USA
    J Bacteriol 180:1277-86. 1998
    ..synthesis is under autogenous control, including response regulator PhoB, its partner, histidine sensor kinase PhoR, all four components of the Pi-specific transport (Pst) system (PstA, PstB, PstC, and PstS), and a protein of ..
  13. ncbi Gene regulation by phosphate in enteric bacteria
    B L Wanner
    Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907
    J Cell Biochem 51:47-54. 1993
    ..Pi control of these genes requires the sensor PhoR, the response regulator PhoB, the binding protein-dependent Pi-specific transporter Pst, and the accessory protein ..
  14. ncbi Regulation of the pho regulon of Escherichia coli K-12. Cloning of the regulatory genes phoB and phoR and identification of their gene products
    J Tommassen
    J Mol Biol 157:265-74. 1982
  15. pmc Mutants affected in alkaline phosphatase, expression: evidence for multiple positive regulators of the phosphate regulon in Escherichia coli
    B L Wanner
    Genetics 96:353-66. 1980
    ..coli is believed to be subject to both positive control by the phoB gene product and negative control by the phoR gene product. We have isolated a large number of PhoA- mutants in the phoR- genetic background...
  16. pmc Outer membrane protein e of Escherichia coli K-12 is co-regulated with alkaline phosphatase
    J Tommassen
    J Bacteriol 143:151-7. 1980
    ..Alternatively, individual phoS, phoT, and phoR mutants as well as pit pst double mutants, all of which are known to produce alkaline phosphatase constitutively, ..
  17. ncbi Regulation of the pho regulon in Escherichia coli K-12. Genetic and physiological regulation of the positive regulatory gene phoB
    H Shinagawa
    J Mol Biol 168:477-88. 1983
    ..Expression of both genes was induced by phosphate starvation. Both genes were constitutively expressed in phoR, phoS, phoT and phoU mutants and were not expressed in a phoR-phoM double mutant...
  18. pmc Inorganic phosphate transport in Escherichia coli: involvement of two genes which play a role in alkaline phosphatase regulation
    G R Willsky
    J Bacteriol 113:529-39. 1973
    ..It is suggested that the phoS and phoT genes are primarily involved in P(i) transport and, as a result of this function, play a role in the regulation of alkaline phosphatase synthesis...
  19. ncbi Nucleotide sequence of the phoR gene, a regulatory gene for the phosphate regulon of Escherichia coli
    K Makino
    J Mol Biol 192:549-56. 1986
    Genes in the phosphate regulon of Escherichia coli are positively regulated by the products of the phoB and phoR genes with limited phosphate, and negatively regulated by the product of the phoR gene with excess phosphate...
  20. pmc Arg-220 of the PstA protein is required for phosphate transport through the phosphate-specific transport system in Escherichia coli but not for alkaline phosphatase repression
    G B Cox
    Department of Biochemistry, John Curtin School of Medical Research, Australian National University, Canberra City
    J Bacteriol 170:2283-6. 1988
    ..Neither the Arg-170----Gln nor the Glu-173----Gln mutation affected phosphate transport. The results are discussed in relation to a proposed structure of the PstA protein...
  21. pmc The phoBR operon in Escherichia coli K-12
    B L Wanner
    Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907
    J Bacteriol 169:5569-74. 1987
    The phoB and phoR genes encode a transcription activator and a sensory protein of the phosphate regulon, respectively. It is shown here that they were transcribed as an operon in which the phoB gene was promoter proximal...
  22. ncbi Signal transduction in the phosphate regulon of Escherichia coli involves phosphotransfer between PhoR and PhoB proteins
    K Makino
    Department of Experimental Chemotherapy, Osaka University, Japan
    J Mol Biol 210:551-9. 1989
    ..b>PhoR protein activates PhoB when phosphate is limiting and inactivates it when phosphate is in excess...
  23. ncbi Regulation of the phosphate regulon of Escherichia coli K-12: regulation and role of the regulatory gene phoR
    K Makino
    J Mol Biol 184:231-40. 1985
    The phoR gene product functions as a negative regulator with excess of phosphate and as a positive regulator with limited phosphate for the phosphate-starvation-inducible pho regulon of Escherichia coli...