nuoH

Summary

Gene Symbol: nuoH
Description: NADH:ubiquinone oxidoreductase, membrane subunit H
Alias: ECK2276, JW2277
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. pmc Real-time electron transfer in respiratory complex I
    Marina L Verkhovskaya
    Helsinki Bioenergetics Group, Structural Biology and Biophysics Program, Institute of Biotechnology, University of Helsinki, 00014 Helsinki, Finland
    Proc Natl Acad Sci U S A 105:3763-7. 2008
  2. doi Conserved lysine residues of the membrane subunit NuoM are involved in energy conversion by the proton-pumping NADH:ubiquinone oxidoreductase (Complex I)
    Liliya Euro
    Helsinki Bioenergetics Group, Institute of Biotechnology, PO Box 65 Viikinkaari 1 FIN 00014 University of Helsinki, Finland
    Biochim Biophys Acta 1777:1166-72. 2008
  3. doi Modeling of human pathogenic mutations in Escherichia coli complex I reveals a sensitive region in the fourth inside loop of NuoH
    Pilvi Maliniemi
    Department of Medical Biochemistry and Molecular Biology, Institute of Biomedicine, University of Oulu, Finland
    Mitochondrion 9:394-401. 2009
  4. doi Disruption of individual nuo-genes leads to the formation of partially assembled NADH:ubiquinone oxidoreductase (complex I) in Escherichia coli
    Heiko Erhardt
    Albert Ludwigs Universitat, Freiburg, Institut für Organische Chemie und Biochemie and Spemann Graduate School of Biology and Medicine, Albertstr 21, 79104 Freiburg i Br, Germany
    Biochim Biophys Acta 1817:863-71. 2012
  5. ncbi Characterization of the NuoM (ND4) subunit in Escherichia coli NDH-1: conserved charged residues essential for energy-coupled activities
    Jesus Torres-Bacete
    Division of Biochemistry, Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, California 92037, USA
    J Biol Chem 282:36914-22. 2007
  6. ncbi A pair of membrane-embedded acidic residues in the NuoK subunit of Escherichia coli NDH-1, a counterpart of the ND4L subunit of the mitochondrial complex I, are required for high ubiquinone reductase activity
    Marko Kervinen
    Department of Medical Biochemistry and Molecular Biology, University of Oulu, Oulu, Finland
    Biochemistry 43:773-81. 2004
  7. doi LHON/MELAS overlap mutation in ND1 subunit of mitochondrial complex I affects ubiquinone binding as revealed by modeling in Escherichia coli NDH-1
    Jukka Pätsi
    Department of Medical Biochemistry and Molecular Biology, University of Oulu, Finland
    Biochim Biophys Acta 1817:312-8. 2012
  8. ncbi The gene locus of the proton-translocating NADH: ubiquinone oxidoreductase in Escherichia coli. Organization of the 14 genes and relationship between the derived proteins and subunits of mitochondrial complex I
    U Weidner
    Institut fur Biochemie, Heinrich Heine Universitat Dusseldorf, Federal Republic of Germany
    J Mol Biol 233:109-22. 1993
  9. ncbi Characterization of the membrane domain subunit NuoJ (ND6) of the NADH-quinone oxidoreductase from Escherichia coli by chromosomal DNA manipulation
    Mou Chieh Kao
    Division of Biochemistry, Department of Molecular and Experimental Medicine, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA
    Biochemistry 44:3562-71. 2005
  10. ncbi Inhibition by capsaicin of NADH-quinone oxidoreductases is correlated with the presence of energy-coupling site 1 in various organisms
    T Yagi
    Department of Molecular and Experimental Medicine, Research Institute of Scripps Clinic, La Jolla, California 92037
    Arch Biochem Biophys 281:305-11. 1990

Scientific Experts

  • T Friedrich
  • Liliya Euro
  • J Steuber
  • Takao Yagi
  • TOMOKO NONE OHNISHI
  • G Unden
  • M I Verkhovsky
  • Thomas Pohl
  • Leonid A Sazanov
  • Marko Kervinen
  • Eiko Nakamaru-Ogiso
  • Akemi Matsuno-Yagi
  • Mou Chieh Kao
  • Pilvi Maliniemi
  • Jukka Pätsi
  • Bilal Amarneh
  • Peter J Holt
  • Ilmo E Hassinen
  • Daniel Schneider
  • Jesus Torres-Bacete
  • Marina L Verkhovskaya
  • David J Morgan
  • Mareike Uhlmann
  • Stefan Stolpe
  • Marten Wikstrom
  • Heiko Erhardt
  • Bettina Bottcher
  • Julia Walter
  • Prem Kumar Sinha
  • Markus Kohlstädt
  • S Bungert
  • Katerina Dörner
  • Daria Esterházy
  • Anja C Gemperli
  • Ekaterina A Baranova
  • Liliya Sinegina
  • Steven B Vik
  • Aygun A Mamedova
  • M Mohiuddin Kabir
  • Moshe Finel
  • J Bongaerts
  • Xing Gong
  • Micaela Hesterberg
  • V Guenebaut
  • Dierk Scheide
  • Pamela David
  • Vera Muders
  • Johanna Uusimaa
  • Thomas Nystrom
  • Stefan Steimle
  • Salla Pakanen
  • Reetta Hinttala
  • Kari Majamaa
  • V Spehr
  • P Hellwig
  • M Braun
  • D Scheide
  • H Leif
  • H Weiss
  • Norma Castro-Guerrero
  • Volker Spehr
  • Martin S King
  • B Wackwitz
  • Y T Yang
  • Petra Hellwig
  • Judy Hirst
  • Gregory Yakovlev
  • Annette Berger
  • Ruth Hielscher
  • Nikolai Belevich
  • Christiane Schaffitzel
  • Miriam Kaufenstein
  • A Schlitt
  • Claude Jakob
  • H J Falk-Krzesinski
  • Jessica De Leon-Rangel
  • Hideto Miyoshi
  • Salvatore Di Bernardo
  • Kazuyuki Shimizu
  • Q H Tran
  • U Weidner
  • Joe Carroll
  • Linda Yu
  • Chang An Yu
  • Tong Xie
  • Marc Baumann
  • M W Calhoun
  • A J Wolfe
  • T Satoh
  • A V Bogachev

Detail Information

Publications69

  1. pmc Real-time electron transfer in respiratory complex I
    Marina L Verkhovskaya
    Helsinki Bioenergetics Group, Structural Biology and Biophysics Program, Institute of Biotechnology, University of Helsinki, 00014 Helsinki, Finland
    Proc Natl Acad Sci U S A 105:3763-7. 2008
    ..Possible consequences of these findings for the proton translocation mechanism are discussed...
  2. doi Conserved lysine residues of the membrane subunit NuoM are involved in energy conversion by the proton-pumping NADH:ubiquinone oxidoreductase (Complex I)
    Liliya Euro
    Helsinki Bioenergetics Group, Institute of Biotechnology, PO Box 65 Viikinkaari 1 FIN 00014 University of Helsinki, Finland
    Biochim Biophys Acta 1777:1166-72. 2008
    ..Remarkably, the subunits NuoL and NuoH in the membrane domain also appear to contain conserved lysine residues in transmembrane helices, which may give a ..
  3. doi Modeling of human pathogenic mutations in Escherichia coli complex I reveals a sensitive region in the fourth inside loop of NuoH
    Pilvi Maliniemi
    Department of Medical Biochemistry and Molecular Biology, Institute of Biomedicine, University of Oulu, Finland
    Mitochondrion 9:394-401. 2009
    ..matrix-side loop of the ND1 subunit by mutating homologous amino acids and surrounding conserved residues of the NuoH subunit of Escherichia coli NDH-1...
  4. doi Disruption of individual nuo-genes leads to the formation of partially assembled NADH:ubiquinone oxidoreductase (complex I) in Escherichia coli
    Heiko Erhardt
    Albert Ludwigs Universitat, Freiburg, Institut für Organische Chemie und Biochemie and Spemann Graduate School of Biology and Medicine, Albertstr 21, 79104 Freiburg i Br, Germany
    Biochim Biophys Acta 1817:863-71. 2012
    ..The inactive population is missing cluster N2 and is tightly associated with the inducible lysine decarboxylase. This article is part of a Special Issue entitled: Biogenesis/Assembly of Respiratory Enzyme Complexes...
  5. ncbi Characterization of the NuoM (ND4) subunit in Escherichia coli NDH-1: conserved charged residues essential for energy-coupled activities
    Jesus Torres-Bacete
    Division of Biochemistry, Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, California 92037, USA
    J Biol Chem 282:36914-22. 2007
    ..The data suggest that these His are not involved in the catalytic Q-binding. Functional roles of NuoM and advantages of NDH-1 research as a model for mitochondrial complex I study have been discussed...
  6. ncbi A pair of membrane-embedded acidic residues in the NuoK subunit of Escherichia coli NDH-1, a counterpart of the ND4L subunit of the mitochondrial complex I, are required for high ubiquinone reductase activity
    Marko Kervinen
    Department of Medical Biochemistry and Molecular Biology, University of Oulu, Oulu, Finland
    Biochemistry 43:773-81. 2004
    ....
  7. doi LHON/MELAS overlap mutation in ND1 subunit of mitochondrial complex I affects ubiquinone binding as revealed by modeling in Escherichia coli NDH-1
    Jukka Pätsi
    Department of Medical Biochemistry and Molecular Biology, University of Oulu, Finland
    Biochim Biophys Acta 1817:312-8. 2012
    ..E24K substitution in ND1 with LHON/MELAS phenotype was modeled here in a homologous position (NuoH-E36K) in the Escherichia coli enzyme and it almost totally abolished complex I activity...
  8. ncbi The gene locus of the proton-translocating NADH: ubiquinone oxidoreductase in Escherichia coli. Organization of the 14 genes and relationship between the derived proteins and subunits of mitochondrial complex I
    U Weidner
    Institut fur Biochemie, Heinrich Heine Universitat Dusseldorf, Federal Republic of Germany
    J Mol Biol 233:109-22. 1993
    ..To some extent, the gene order correlates with the topological arrangement of the encoded subunits. The conception of modular evolution of NADH: ubiquinone oxidoreductase is further supported by the arrangement of the nuo-genes...
  9. ncbi Characterization of the membrane domain subunit NuoJ (ND6) of the NADH-quinone oxidoreductase from Escherichia coli by chromosomal DNA manipulation
    Mou Chieh Kao
    Division of Biochemistry, Department of Molecular and Experimental Medicine, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA
    Biochemistry 44:3562-71. 2005
    ..Together with the results on mutations related to human diseases, possible functional roles of the NuoJ subunit have been discussed...
  10. ncbi Inhibition by capsaicin of NADH-quinone oxidoreductases is correlated with the presence of energy-coupling site 1 in various organisms
    T Yagi
    Department of Molecular and Experimental Medicine, Research Institute of Scripps Clinic, La Jolla, California 92037
    Arch Biochem Biophys 281:305-11. 1990
    ..The mechanism by which capsaicin inhibits the energy-transducing NADH-quinone oxidoreductase is discussed...
  11. ncbi EPR signals assigned to Fe/S cluster N1c of the Escherichia coli NADH:ubiquinone oxidoreductase (complex I) derive from cluster N1a
    Mareike Uhlmann
    Albert Ludwigs Universitat Freiburg, Institut fur Organische Chemie und Biochemie, Albertstrasse 21, Chemiehochhaus, D 79104 Freiburg im Breisgau, Germany
    Biochemistry 44:1653-8. 2005
    ..Thus, there is no third binuclear iron-sulfur "N1c" in the E. coli complex I but an additional tetranuclear cluster that may be coined N7...
  12. ncbi Metabolic regulation analysis of icd-gene knockout Escherichia coli based on 2D electrophoresis with MALDI-TOF mass spectrometry and enzyme activity measurements
    M Mohiuddin Kabir
    Department of Biochemical Engineering and Science, Kyushu Institute of Technology, Iizuka 820 8502, Fukuoka, Japan
    Appl Microbiol Biotechnol 65:84-96. 2004
    ..activity significantly affected the respiratory system and electron transport chain, as evidenced by the significant downregulation of proteins encoded by the genes nuoE, nuoH, cydA and cyoA in icd mutant E. coli compared to the parent.
  13. ncbi Substrate-induced conformational change in bacterial complex I
    Aygun A Mamedova
    Medical Research Council Dunn Human Nutrition Unit, Wellcome Trust Medical Research Council Building, Cambridge CB2 2XY, United Kingdom
    J Biol Chem 279:23830-6. 2004
    ..The enzyme retains its L-shape in the presence of NADH, but exhibits a significantly more open or expanded structure both in the peripheral arm and, unexpectedly, in the membrane domain also...
  14. ncbi The Escherichia coli NADH:ubiquinone oxidoreductase (complex I) is a primary proton pump but may be capable of secondary sodium antiport
    Stefan Stolpe
    Institut fur Organische Chemie und Biochemie, Albert Ludwigs Universitat Freiburg, Albertstrasse 21, D 79104 Freiburg, Germany
    J Biol Chem 279:18377-83. 2004
    ..coli complex I is a primary electrogenic proton pump. However, the magnitude of the pH gradient depended on the sodium concentration. The capability of complex I for secondary Na(+)/H(+) antiport is discussed...
  15. ncbi The gross structure of the respiratory complex I: a Lego System
    Thorsten Friedrich
    Institut fur Organische Chemie und Biochemie, Albert Ludwigs Universitat Freiburg, Albertstr 21, D 79104 Freiburg, Germany
    Biochim Biophys Acta 1608:1-9. 2004
    ..This model reflects the evolution of complex I from pre-existing modules for electron transfer and proton translocation...
  16. ncbi The location of NuoL and NuoM subunits in the membrane domain of the Escherichia coli complex I: implications for the mechanism of proton pumping
    Peter J Holt
    Medical Research Council Dunn Human Nutrition Unit, Wellcome Trust MRC Building, Hills Road, Cambridge CB2 2XY, United Kingdom
    J Biol Chem 278:43114-20. 2003
    ..or Triton X-100 led to further disruption of the membrane domain into fragments containing NuoL/M/N, NuoA/K/N, and NuoH/J subunits...
  17. ncbi The C-terminally truncated NuoL subunit (ND5 homologue) of the Na+-dependent complex I from Escherichia coli transports Na+
    Julia Steuber
    Mikrobiologisches Institut der Eidgenössischen Technischen Hochschule, ETH Zentrum, Schmelzbergstrasse 7, CH 8092 Zurich, Switzerland
    J Biol Chem 278:26817-22. 2003
    ..This Na+ uptake was prevented by EIPA (5-(N-ethyl-N-isopropyl)-amiloride), which acts as inhibitor against Na+/H+ antiporters...
  18. ncbi The ubiquinone-binding site in NADH:ubiquinone oxidoreductase from Escherichia coli
    Xing Gong
    Department of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater, Oklahoma 74078, USA
    J Biol Chem 278:25731-7. 2003
    ..Using the PHDhtm hydropathy plot, the labeled peptide is located in the transmembrane helix 4 toward the periplasmic side of the membrane...
  19. ncbi Mutagenesis of subunit N of the Escherichia coli complex I. Identification of the initiation codon and the sensitivity of mutants to decylubiquinone
    Bilal Amarneh
    Department of Biological Sciences, Southern Methodist University, Dallas, Texas 75275 0376, USA
    Biochemistry 42:4800-8. 2003
    ..These mutants also showed enhanced inhibition by decylubiquinone, indicating that subunit N interacts with quinones. The mutation associated with LHON, G391S, had little effect on these functions...
  20. ncbi The proton-translocating NADH-quinone oxidoreductase in the respiratory chain: the secret unlocked
    Takao Yagi
    Department of Molecular and Experimental Medicine, MEM 256, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA
    Biochemistry 42:2266-74. 2003
  21. ncbi Interaction of purified NDH-1 from Escherichia coli with ubiquinone analogues
    Pamela David
    Helsinki Bioenergetics Group, Biotechnology Institute, Biocenter 2, University of Helsinki, Helsinki, Finland
    Biochim Biophys Acta 1553:268-78. 2002
    ..Both ubiquinone-2 and decylubiquinone are good acceptors for this enzyme, while affinity of NDH-1 for ubiquinone-1 is clearly lower than for the other two, particularly in the purified state...
  22. ncbi A novel, enzymatically active conformation of the Escherichia coli NADH:ubiquinone oxidoreductase (complex I)
    Bettina Bottcher
    Structural Biology and Biocomputing Programme, European Molecular Biology Laboratory, Meyerhofstrasse 1, D 69117 Heidelberg, Germany
    J Biol Chem 277:17970-7. 2002
    ..Only the horseshoe-shaped complex I exhibits enzyme activity in detergent solution, which is abolished by the addition of salt. Therefore, it is proposed that this structure is the native conformation of the complex in the membrane...
  23. ncbi Activation of isolated NADH:ubiquinone reductase I (complex I) from Escherichia coli by detergent and phospholipids. Recovery of ubiquinone reductase activity and changes in EPR signals of iron-sulfur clusters
    Liliya Sinegina
    Helsinki Bioenergetics Group, Institute of Biotechnology, PO Box 65 Viikinkaari 1 FIN 00014 University of Helsinki, Finland
    Biochemistry 44:8500-6. 2005
    ..895, 1.904, 2.05, which corresponds to the parameters reported for the N2 cluster. This data indicates conformational rearrangements of catalytic importance in complex I upon binding of phospholipids...
  24. ncbi Characterization of the membrane domain subunit NuoK (ND4L) of the NADH-quinone oxidoreductase from Escherichia coli
    Mou Chieh Kao
    Division of Biochemistry, Department of Molecular and Experimental Medicine, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA
    Biochemistry 44:9545-54. 2005
    ..Possible roles of these arginine residues and other conserved residues in the NuoK subunit for NDH-1 function were discussed...
  25. ncbi Projection structure of the membrane domain of Escherichia coli respiratory complex I at 8 A resolution
    Ekaterina A Baranova
    Medical Research Council Dunn Human Nutrition Unit, Wellcome Trust MRC Building, Hills Road, Cambridge CB2 2XY, UK
    J Mol Biol 366:140-54. 2007
    ....
  26. ncbi Respiratory complex I: mechanistic and structural insights provided by the crystal structure of the hydrophilic domain
    Leonid A Sazanov
    Medical Research Council Dunn Human Nutrition Unit, Wellcome Trust MRC Building, Hills Road, Cambridge CB2 2XY, UK sazanov mrc dunn cam ac uk
    Biochemistry 46:2275-88. 2007
    ..In this review, novel mechanistic implications of the structure are discussed, and the effects of many known mutations of complex I subunits are interpreted in a structural context...
  27. ncbi Lambda Red-mediated mutagenesis and efficient large scale affinity purification of the Escherichia coli NADH:ubiquinone oxidoreductase (complex I)
    Thomas Pohl
    Institut fur Organische Chemie und Biochemie, Albert Ludwigs Universitat, Albertstrasse 21, Spemann Graduate School of Biology and Medicine, D 79104 Freiburg i Br, Germany
    Biochemistry 46:10694-702. 2007
    ..After reconstitution in proteoliposomes it couples the electron transfer with proton translocation in an inhibitor sensitive manner, thus meeting all prerequisites for structural and functional studies...
  28. doi Electrostatic interactions between FeS clusters in NADH:ubiquinone oxidoreductase (Complex I) from Escherichia coli
    Liliya Euro
    Helsinki Bioenergetics Group, Institute of Biotechnology, P O Box 65, Viikinkaari 1 00014 University of Helsinki, Helsinki, Finland
    Biochemistry 47:3185-93. 2008
    ..The observed redox titration curves are discussed in terms of intrinsic electrostatic interactions between FeS centers in complex I. A model showing shifts of E m due to the electrostatic interaction between the centers is presented...
  29. doi Production of reactive oxygen species by complex I (NADH:ubiquinone oxidoreductase) from Escherichia coli and comparison to the enzyme from mitochondria
    Daria Esterházy
    Medical Research Council Dunn Human Nutrition Unit, Wellcome Trust MRC Building, Hills Road, Cambridge, CB2 0XY, United Kingdom
    Biochemistry 47:3964-71. 2008
    ..In contrast, bovine complex I produces 95% superoxide. The results are consistent with (but do not prove) a specific role for cluster N1a in determining the outcome of O2 reduction; possible reaction mechanisms are discussed...
  30. doi Assembly of the Escherichia coli NADH:ubiquinone oxidoreductase (complex I)
    Daniel Schneider
    Institut fur Organische Chemie und Biochemie, Albert Ludwigs Universitat Freiburg, Albertstr 21, 79104 Freiburg, Germany
    Biochim Biophys Acta 1777:735-9. 2008
    ..It is discussed whether this fragment represents an assembly intermediate. In addition, a membrane-bound fragment exhibiting NADH/ferricyanide oxidoreductase activity and containing the iron-sulfur cluster N2 was detected in one mutant...
  31. doi Three-dimensional structure of respiratory complex I from Escherichia coli in ice in the presence of nucleotides
    David J Morgan
    Medical Research Council, Dunn Human Nutrition Unit, Wellcome Trust MRC Building, Hills Road, Cambridge CB2 2XY, UK
    Biochim Biophys Acta 1777:711-8. 2008
    ..The model of the entire bacterial complex I could be built from the crystal structures of subcomplexes using the EM envelope described here...
  32. doi Nucleotide-induced conformational changes in the Escherichia coli NADH:ubiquinone oxidoreductase (complex I)
    Thomas Pohl
    Institut fur Organische Chemie und Biochemie, Albert Ludwigs Universitat, Albertstrasse 21, D 79104 Freiburg, Germany
    Biochem Soc Trans 36:971-5. 2008
    ..EPR spectroscopy of surface mutants of the complex containing a covalently bound spin label at distinct positions demonstrates NADH-dependent conformational changes in both arms of the complex...
  33. doi The role of the invariant glutamate 95 in the catalytic site of Complex I from Escherichia coli
    Liliya Euro
    Helsinki Bioenergetics Group, Institute of Biotechnology, University of Helsinki, Finland
    Biochim Biophys Acta 1787:68-73. 2009
    ....
  34. doi High affinity cation-binding sites in Complex I from Escherichia coli
    Liliya Euro
    Helsinki Bioenergetics Group, Institute of Biotechnology, PO Box 65 Viikinkaari 1 00014 University of Helsinki, Finland
    Biochim Biophys Acta 1787:1024-8. 2009
    ..K(+) and La(3+) do not occupy the same site. Possible localization of these metal-binding sites and their implication in catalysis are discussed...
  35. ncbi Comparison of the inhibitory action of synthetic capsaicin analogues with various NADH-ubiquinone oxidoreductases
    T Satoh
    Department of Agricultural Chemistry, Kyoto University, Japan
    Biochim Biophys Acta 1273:21-30. 1996
    ..1990) Arch. Biochem. Biophys. 281, 305-311). It is noteworthy that several synthetic capsaicins discriminated between NDH-1 and NDH-2 much better than natural capsaicin...
  36. ncbi Purification of NADH-ferricyanide dehydrogenase and NADH-quinone reductase from Escherichia coli membranes and their roles in the respiratory chain
    M Hayashi
    Laboratory of Membrane Biochemistry, Faculty of Pharmaceutical Sciences, Chiba University, Japan
    Biochim Biophys Acta 977:62-9. 1989
    ..The FAD-containing NQR was very similar to that purified by Jaworowski et al. (Biochemistry (1981) 20, 2041-2047), and reduced Q1 without generating delta psi...
  37. ncbi NADH-ubiquinone oxidoreductases of the Escherichia coli aerobic respiratory chain
    K Matsushita
    Roche Institute of Molecular Biology, Roche Research Center, Nutley, New Jersey 07110
    Biochemistry 26:7732-7. 1987
    ....
  38. ncbi Liposomal cyclosporine. Comparison of drug and lipid carrier pharmacokinetics and biodistribution
    E Choice
    Department of Pharmacology, University of British Columbia, Vancouver, Canada
    Transplantation 60:1006-11. 1995
    ....
  39. ncbi Transcriptional regulation of the proton translocating NADH dehydrogenase genes (nuoA-N) of Escherichia coli by electron acceptors, electron donors and gene regulators
    J Bongaerts
    Institut für Mikrobiologie und Weinforschung, Universitat Mainz, Germany
    Mol Microbiol 16:521-34. 1995
    ..A physiological role for the transcriptional stimulation by O2 and nitrate is suggested...
  40. ncbi Isolation and characterization of the proton-translocating NADH: ubiquinone oxidoreductase from Escherichia coli
    H Leif
    Institut fur Biochemie, Heinrich Heine Universitat Dusseldorf, Germany
    Eur J Biochem 230:538-48. 1995
    ..This subunit arrangement coincidences to some extent with the order of the genes on the nuo operon. A topological model of the E. coli complex I is proposed...
  41. pmc Transcriptional control of the nuo operon which encodes the energy-conserving NADH dehydrogenase of Salmonella typhimurium
    C D Archer
    Department of Microbiology, University of Alabama at Birmingham 35294, USA
    J Bacteriol 177:2335-42. 1995
    ..Mutations in the global regulatory genes arcA, oxrA (fnr), crp, cya, and katF were tested for effects on expression of the nuo operon. However, none of the mutations tested had a large effect on expression of type I NADH dehydrogenase...
  42. ncbi The energetics of bacterial growth: a reassessment
    O M Neijssel
    Department of Microbiology, E C Slater Institute, BioCentrum Amsterdam, University of Amsterdam, The Netherlands
    Mol Microbiol 13:172-82. 1994
    ..The different strains indeed show different growth efficiencies. The physiological significance of energetically less-efficient branches of the respiratory chain is discussed...
  43. ncbi Escherichia coli NADH dehydrogenase I, a minimal form of the mitochondrial complex I
    H Leif
    Institute fur Biochemie, Heinrich Heine Universitat Dusseldorf, Germany
    Biochem Soc Trans 21:998-1001. 1993
  44. ncbi Attempts to define distinct parts of NADH:ubiquinone oxidoreductase (complex I)
    T Friedrich
    Heinrich Heine Universitat Dusseldorf, Institut fur Biochemie, Germany
    J Bioenerg Biomembr 25:331-7. 1993
    ..This assumption is further supported by the conserved order of bacterial complex I genes, which correlates with the topological arrangement of the corresponding subunits in the two parts of complex I...
  45. ncbi Studies on the proton-translocating NADH:ubiquinone oxidoreductases of mitochondria and Escherichia coli using the inhibitor 1,10-phenanthroline
    M Finel
    Helsinki Bioenergetics Group, Department of Medical Chemistry, University of Helsinki, Finland
    FEBS Lett 339:142-6. 1994
    ..EPR spectroscopy of membranous E. coli NDH1 shows that two slow- and one fast-relaxing Fe-S clusters become detectable upon reduction by NADH in the presence of OP. However, none of them resembles the mitochondrial cluster 2...
  46. pmc Escherichia coli mutants lacking NADH dehydrogenase I have a competitive disadvantage in stationary phase
    M M Zambrano
    Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts 02115
    J Bacteriol 175:5642-7. 1993
    ..This is the first identification of genes encoding subunits of NADH dehydrogenase I in E. coli. The significance of the inability of these mutant strains to compete in stationary-phase cultures is discussed...
  47. pmc Demonstration of separate genetic loci encoding distinct membrane-bound respiratory NADH dehydrogenases in Escherichia coli
    M W Calhoun
    School of Chemical Sciences, University of Illinois, Urbana 61801
    J Bacteriol 175:3013-9. 1993
    ..The enzyme encoded by this locus probably translocates protons across the inner membrane, contributing to the proton motive force...
  48. pmc Energetic efficiency of Escherichia coli: effects of mutations in components of the aerobic respiratory chain
    M W Calhoun
    School of Chemical Sciences, University of Illinois, Urbana
    J Bacteriol 175:3020-5. 1993
    ....
  49. ncbi Complex I: a chimaera of a redox and conformation-driven proton pump?
    T Friedrich
    Institut fur Biochemie, Universitat Dusseldorf, Germany
    J Bioenerg Biomembr 33:169-77. 2001
    ..This implies that complex I contains two energy-coupling sites. The NADH dehydrogenase module seems to be involved in electron transfer and not in proton translocation...
  50. ncbi The NADH:ubiquinone oxidoreductase (complex I) from Escherichia coli
    T Friedrich
    Institut fur Biochemie, Universitat Dusseldorf, Universitatsstr 1, D 40225 Dusseldorf, Germany
    Biochim Biophys Acta 1364:134-46. 1998
  51. ncbi FT-IR spectroscopic characterization of NADH:ubiquinone oxidoreductase (complex I) from Escherichia coli: oxidation of FeS cluster N2 is coupled with the protonation of an aspartate or glutamate side chain
    P Hellwig
    Institut fur Biophysik, Johann Wolfgang Goethe Universität Theodor Stern Kai 7, Haus 74, 60590 Frankfurt M, Germany
    Biochemistry 39:10884-91. 2000
    ..Part of these signals are attributed to the reorganization of protonated/deprotonated Asp or Glu side chains. On the basis of these data we discuss the role of N2 for proton translocation of complex I...
  52. ncbi The respiratory complex I of bacteria, archaea and eukarya and its module common with membrane-bound multisubunit hydrogenases
    T Friedrich
    Institut fur Biochemie, Heinrich Heine Universitat Dusseldorf, Germany
    FEBS Lett 479:1-5. 2000
    ..Six of them are also present in a family of membrane-bound multisubunit [NiFe] hydrogenases. It is discussed that they build a module for electron transfer coupled to proton translocation...
  53. ncbi Na+ translocation by complex I (NADH:quinone oxidoreductase) of Escherichia coli
    J Steuber
    Mikrobiologisches Institut der Eidgenössischen Technischen Hochschule, ETH Zentrum, Schmelzbergstr 7, CH 8092 Zurich, Switzerland
    Mol Microbiol 35:428-34. 2000
    ..With an E. coli mutant deficient in complex I, the Na+ transport activity was low (1-3 nmol mg-1 min-1), and rotenone was without effect...
  54. ncbi Growth phase-dependent regulation of nuoA-N expression in Escherichia coli K-12 by the Fis protein: upstream binding sites and bioenergetic significance
    B Wackwitz
    Institut für Mikrobiologie und Weinforschung, Universitat Mainz, Germany
    Mol Gen Genet 262:876-83. 1999
    ..This ensures higher ATP yields under conditions where large amounts of ATP are required...
  55. ncbi Overexpression of the Escherichia coli nuo-operon and isolation of the overproduced NADH:ubiquinone oxidoreductase (complex I)
    V Spehr
    Institut fur Biochemie, Heinrich Heine Universitat, Dusseldorf, Germany
    Biochemistry 38:16261-7. 1999
    ..Due to its stability over a wide pH range and at very high salt concentrations, this preparation is well suited for structural investigations...
  56. ncbi One-step purification of the NADH dehydrogenase fragment of the Escherichia coli complex I by means of Strep-tag affinity chromatography
    S Bungert
    Heinrich Heine Universitat Dusseldorf, Institut fur Biochemie, Universitatsstr 1, D 40225, Dusseldorf, Germany
    FEBS Lett 460:207-11. 1999
    ..This was achieved by fusing the Strep-tag II peptide to the C-terminus of NuoF or NuoG. Fusion of this peptide to the N-terminus of either NuoE or NuoF disturbed the assembly of the NADH dehydrogenase fragment...
  57. ncbi Effect of inactivation of nuo and ackA-pta on redistribution of metabolic fluxes in Escherichia coli
    Y T Yang
    Departments of Bioengineering and Chemical Engineering, Rice University, PO Box 1892, MS 142, Houston, Texas 77251 1892, USA
    Biotechnol Bioeng 65:291-7. 1999
    ..Mutations in both ackA-pta and nuo are required to significantly reduce the flux through the PFL pathway...
  58. ncbi Iron-sulfur clusters/semiquinones in complex I
    T Ohnishi
    Johnson Research Foundation, Department of Biochemistry and Biophysics, and the University of Pennsylvania, Philadelphia, PA 19104, USA
    Biochim Biophys Acta 1364:186-206. 1998
    ..A brief introduction of EPR technique was also described in Appendix A of this mini-review...
  59. ncbi Consistent structure between bacterial and mitochondrial NADH:ubiquinone oxidoreductase (complex I)
    V Guenebaut
    Structural Biology and Biocomputing Programme, European Molecular Biology Laboratory, Heidelberg, Germany
    J Mol Biol 276:105-12. 1998
    ....
  60. pmc Genetic analysis of the nuo locus, which encodes the proton-translocating NADH dehydrogenase in Escherichia coli
    H J Falk-Krzesinski
    Department of Microbiology and Immunology, Loyola University Chicago Stritch School of Medicine, Maywood, Illinois 60153, USA
    J Bacteriol 180:1174-84. 1998
    ..In particular, we present evidence that NuoG, a peripheral subunit, is essential for complex I function and that it plays a role in the regulation of nuo expression and/or the assembly of complex I...
  61. ncbi Characterization of the overproduced NADH dehydrogenase fragment of the NADH:ubiquinone oxidoreductase (complex I) from Escherichia coli
    M Braun
    Institut fur Biochemie, Heinrich Heine Universitat, Dusseldorf, Germany
    Biochemistry 37:1861-7. 1998
    ..The preparation fulfills all prerequisites for crystallization of the fragment...
  62. ncbi Alternative respiratory pathways of Escherichia coli: energetics and transcriptional regulation in response to electron acceptors
    G Unden
    Institut für Mikrobiologie und Weinforschung, Universitat Mainz, Germany
    Biochim Biophys Acta 1320:217-34. 1997
    ..Reductive activation could be achieved by cellular reductants in the absence of O2. In addition, O2 may cause destruction and loss of the FeS cluster. It is not known whether this process is required for regulation of FNR function...
  63. ncbi Requirement for the proton-pumping NADH dehydrogenase I of Escherichia coli in respiration of NADH to fumarate and its bioenergetic implications
    Q H Tran
    Institut für Mikrobiologie und Weinforschung, Universitat Mainz, Germany
    Eur J Biochem 244:155-60. 1997
    ..NADH-->dimethylsulfoxide respiration is also dependent on NADH dehydrogenase I. The consequences for energy conservation by anaerobic respiration with NADH as a donor are discussed...
  64. pmc H+/e- stoichiometry for NADH dehydrogenase I and dimethyl sulfoxide reductase in anaerobically grown Escherichia coli cells
    A V Bogachev
    Department of Bioenergetics, A N Belozersky Institute of Physico Chemical Biology, Moscow State University, Russia
    J Bacteriol 178:6233-7. 1996
    ..These data suggest that (i) the H+/e- stoichiometry for E. coli NDH-I is at least 1.5 and (ii) the DMSO reductase does not generate a proton motive force...
  65. ncbi Construction of a deletion strain and expression vector for the Escherichia coli NADH:ubiquinone oxidoreductase (Complex I)
    Bilal Amarneh
    Department of Biological Sciences, Southern Methodist University, Dallas, TX 75275 0376, USA
    Biochim Biophys Acta 1757:1557-60. 2006
    ..A chromosomal deletion of all nuo genes has been achieved by homologous recombination. A vector that encodes all of the nuo genes has been constructed, and it expresses a functional enzyme...
  66. pmc Mutations in NADH:ubiquinone oxidoreductase of Escherichia coli affect growth on mixed amino acids
    B M Prüss
    Department of Microbiology and Immunology, Loyola University Chicago, Maywood, Illinois 60153
    J Bacteriol 176:2143-50. 1994
    ..We propose that cells defective for NADH dehydrogenase I exhibit all these phenotypes, because large NADH/NAD+ ratios inhibit certain tricarboxylic acid cycle enzymes, e.g., citrate synthase and malate dehydrogenase...
  67. pmc Critical roles of subunit NuoH (ND1) in the assembly of peripheral subunits with the membrane domain of Escherichia coli NDH-1
    Prem Kumar Sinha
    Division of Biochemistry, Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, California 92037, USA
    J Biol Chem 284:9814-23. 2009
    ..b>NuoH is a counterpart of ND1, which is one of seven mitochondrially encoded hydrophobic subunits, and is considered to ..
  68. ncbi Transport of Na(+) and K (+) by an antiporter-related subunit from the Escherichia coli NADH dehydrogenase I produced in Saccharomyces cerevisiae
    Anja C Gemperli
    Department of Biochemistry, University of Zurich, Winterthurerstrasse 190, 8057, Zurich, Switzerland
    Arch Microbiol 188:509-21. 2007
    ..The cation selectivity and function of the NuoL subunit as a transporter module of the NADH dehydrogenase complex is discussed...

Research Grants5

  1. Development of therapies to retard Parkinson's disease
    Takao Yagi; Fiscal Year: 2003
    ..2) Effects of MPTP on transgenic mice expressing the Ndil enzyme in the dopamine-producing tissues. ..
  2. Development of therapies to retard Parkinson's disease
    Takao Yagi; Fiscal Year: 2008
    ..2) Suppression of PD's disease like symptoms in rodent models by the Ndi1 expression. ..
  3. MOLECULAR REMEDY OF MITOCHONDRIAL DEFECTS
    Takao Yagi; Fiscal Year: 2009
    ..2) Construction of animal models for human diseases caused by complex I defects. (3) Protection by the Ndi1 enzyme against tissue degradation caused by complex I deficiencies. ..
  4. NADH-UBIQUINONE REDUCTASE OF PARACOCCUS DENITRIFICANS
    Takao Yagi; Fiscal Year: 2011
    ..In order to develop therapies for diseases caused by deficiencies of this enzyme, it is a prerequisite to investigate why and how defects occur. ..
  5. PROTON & ELECTRON TRANSFER & ENERGY COUPLING IN SITE I
    TOMOKO NONE OHNISHI; Fiscal Year: 2010
    ..Using this new strategy, we found that both cluster N2 and flavin can generate superoxide. We will solidify this exciting finding and study its relevance to complex I function under physiological and pathological conditions. ..