nth

Summary

Gene Symbol: nth
Description: DNA glycosylase and apyrimidinic (AP) lyase (endonuclease III)
Alias: ECK1629, JW1625
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. ncbi Substrate specificity of the Escherichia coli endonuclease III: excision of thymine- and cytosine-derived lesions in DNA produced by radiation-generated free radicals
    M Dizdaroglu
    Chemical Science and Technology Laboratory, National Institute of Standards and Technology, Gaithersburg, Maryland 20899
    Biochemistry 32:12105-11. 1993
  2. ncbi New substrates for old enzymes. 5-Hydroxy-2'-deoxycytidine and 5-hydroxy-2'-deoxyuridine are substrates for Escherichia coli endonuclease III and formamidopyrimidine DNA N-glycosylase, while 5-hydroxy-2'-deoxyuridine is a substrate for uracil DNA N-glycos
    Z Hatahet
    Department of Microbiology and Molecular Genetics, University of Vermont, Burlington 05405
    J Biol Chem 269:18814-20. 1994
  3. ncbi Differential specificity of human and Escherichia coli endonuclease III and VIII homologues for oxidative base lesions
    Atsushi Katafuchi
    Department of Mathematical and Life Sciences, Graduate School of Science, Hiroshima University, Higashi Hiroshima 739 8526, Japan
    J Biol Chem 279:14464-71. 2004
  4. ncbi Novel substrates of Escherichia coli nth protein and its kinetics for excision of modified bases from DNA damaged by free radicals
    M Dizdaroglu
    Chemical Science and Technology Laboratory, National Institute of Standards and Technology, Gaithersburg, Maryland 20899 8311, USA
    Biochemistry 39:5586-92. 2000
  5. pmc Stereoselective excision of thymine glycol from oxidatively damaged DNA
    Holly Miller
    Laboratory of Chemical Biology, State University of New York, Stony Brook, NY, USA
    Nucleic Acids Res 32:338-45. 2004
  6. ncbi Inhibition of DNA repair glycosylases by base analogs and tryptophan pyrolysate, Trp-P-1
    Elzbieta Speina
    Department of Molecular Biology, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warszawa, Poland
    Acta Biochim Pol 52:167-78. 2005
  7. ncbi Stereochemical studies of the beta-elimination reactions at aldehydic abasic sites in DNA: endonuclease III from Escherichia coli, sodium hydroxide, and Lys-Trp-Lys
    A Mazumder
    Department of Chemistry and Biochemistry, University of Maryland, College Park 20742
    Biochemistry 30:1119-26. 1991
  8. ncbi Endonuclease III is an iron-sulfur protein
    R P Cunningham
    Department of Biological Sciences, State University of New York, Albany 12222
    Biochemistry 28:4450-5. 1989
  9. ncbi Mechanism of action of Escherichia coli endonuclease III
    Y W Kow
    Department of Microbiology and Immunology, New York Medical College, Valhalla 10595
    Biochemistry 26:8200-6. 1987
  10. pmc Importance of thiols in the repair mechanisms of DNA containing AP (apurinic or apyrimidinic) sites
    V Bailly
    Department of Biochemistry, Faculty of Sciences, University of Liege, Belgium
    Nucleic Acids Res 16:9489-96. 1988

Scientific Experts

  • Keizo Tano
  • Martine E Lomax
  • H Miller
  • Miral Dizdaroglu
  • C Janion
  • M Takao
  • Dee R Denver
  • J R Wagner
  • Amie K Boal
  • Jacqueline K Barton
  • Tetsuya Suzuki
  • Elzbieta Speina
  • S S Wallace
  • Shigenori Iwai
  • Hiroshi Ide
  • Atsushi Katafuchi
  • Christine A Romano
  • Anna Sikora
  • J Laval
  • Pamela A Sontz
  • C M Gifford
  • Guoqiang Tan
  • Naoyuki Matsumoto
  • Hideyoshi Harashima
  • Hiroyuki Kamiya
  • Kazuo Yamamoto
  • Yusuke Doi
  • Eylon Yavin
  • Takashi Watanabe
  • Carissa J Wiederholt
  • P W Chang
  • Claudia Alessandra Fortes Aiub
  • Marc M Greenberg
  • Barbara Tudek
  • R P Cunningham
  • A A Purmal
  • Anton B Guliaev
  • V Lhiaubet-Vallet
  • Kelly M Kroeger
  • Z Hatahet
  • Damian Mielecki
  • Michal Wrzesinski
  • M H David-Cordonnier
  • Elzbieta Grzesiuk
  • Jadwiga Nieminuszczy
  • C V Privezentzev
  • Aleksandra Chojnacka
  • Jeffrey A Gralnick
  • Ryosuke Hayashi
  • Masayuki Himoto
  • Jianxin Lu
  • Sayuri Morita
  • Fuki Hagiwara
  • Isao Kuraoka
  • Huangen Ding
  • Jacob P Bitoun
  • Katsuo Katayanagi
  • Dianne K Newman
  • Hao Huang
  • Joseph C Genereux
  • M Saparbaev
  • J P Banath
  • C D'Ham
  • M M Greenberg
  • D M Serafini
  • Yoshie Fujiwara
  • J Jurado
  • Kenichi Hitomi
  • L Harrison
  • John A Tainer
  • Yu Lin Jiang
  • K Takatori
  • Jacques Laval
  • Maria Anna Graziewicz
  • Jarosław M Cieśla
  • Kazuhiro Haraguchi
  • S Yonei
  • Olga A Lukianova
  • Valerie L O'Shea
  • Jennifer N Patro
  • Sheila S David
  • Jeffrey O Blaisdell
  • Jeffrey P Bond
  • Susan S Wallace
  • Zygmunt Kazimierczuk
  • Q M Zhang
  • A Tachibana
  • Luis Felipe Ribeiro Pinto
  • Toshiaki Nakano
  • Bo Hang

Detail Information

Publications67

  1. ncbi Substrate specificity of the Escherichia coli endonuclease III: excision of thymine- and cytosine-derived lesions in DNA produced by radiation-generated free radicals
    M Dizdaroglu
    Chemical Science and Technology Laboratory, National Institute of Standards and Technology, Gaithersburg, Maryland 20899
    Biochemistry 32:12105-11. 1993
    ..It is the first investigation of the substrate specificity of this repair enzyme in the context of a large number of pyrimidine- and purine-derived lesions in DNA...
  2. ncbi New substrates for old enzymes. 5-Hydroxy-2'-deoxycytidine and 5-hydroxy-2'-deoxyuridine are substrates for Escherichia coli endonuclease III and formamidopyrimidine DNA N-glycosylase, while 5-hydroxy-2'-deoxyuridine is a substrate for uracil DNA N-glycos
    Z Hatahet
    Department of Microbiology and Molecular Genetics, University of Vermont, Burlington 05405
    J Biol Chem 269:18814-20. 1994
    ..Analysis of crude extracts obtained from wild type and endonuclease III deletion mutants of E. coli correlated well with data obtained with the purified enzymes...
  3. ncbi Differential specificity of human and Escherichia coli endonuclease III and VIII homologues for oxidative base lesions
    Atsushi Katafuchi
    Department of Mathematical and Life Sciences, Graduate School of Science, Hiroshima University, Higashi Hiroshima 739 8526, Japan
    J Biol Chem 279:14464-71. 2004
    ..However, detailed analysis of the cellular activity suggests that hNEIL1 has a significant role in the repair of 5S-Tg in human cells...
  4. ncbi Novel substrates of Escherichia coli nth protein and its kinetics for excision of modified bases from DNA damaged by free radicals
    M Dizdaroglu
    Chemical Science and Technology Laboratory, National Institute of Standards and Technology, Gaithersburg, Maryland 20899 8311, USA
    Biochemistry 39:5586-92. 2000
    Escherichia coli Nth protein (endonuclease III) is a DNA glycosylase with a broad substrate specificity for pyrimidine derivatives. We discovered novel substrates of E...
  5. pmc Stereoselective excision of thymine glycol from oxidatively damaged DNA
    Holly Miller
    Laboratory of Chemical Biology, State University of New York, Stony Brook, NY, USA
    Nucleic Acids Res 32:338-45. 2004
    ..When DNA glycosylases occur as complementary pairs, failure of one or both enzymes to excise their cognate Tg stereoisomer from oxidatively damaged DNA could have deleterious consequences for the cell...
  6. ncbi Inhibition of DNA repair glycosylases by base analogs and tryptophan pyrolysate, Trp-P-1
    Elzbieta Speina
    Department of Molecular Biology, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warszawa, Poland
    Acta Biochim Pol 52:167-78. 2005
    ..on the activity of base excision repair enzymes: Escherichia coli and human DNA glycosylases of oxidized (Fpg, Nth) and alkylated bases (TagA, AlkA, and ANPG), and for bacterial AP endonuclease (Xth protein)...
  7. ncbi Stereochemical studies of the beta-elimination reactions at aldehydic abasic sites in DNA: endonuclease III from Escherichia coli, sodium hydroxide, and Lys-Trp-Lys
    A Mazumder
    Department of Chemistry and Biochemistry, University of Maryland, College Park 20742
    Biochemistry 30:1119-26. 1991
    ....
  8. ncbi Endonuclease III is an iron-sulfur protein
    R P Cunningham
    Department of Biological Sciences, State University of New York, Albany 12222
    Biochemistry 28:4450-5. 1989
    ..94 type EPR signal characteristic of a [4Fe-4S]1+ cluster. These studies show that endonuclease III is unique in being both a DNA repair enzyme and an iron-sulfur protein. The function of the 4Fe-4S cluster remains to be established...
  9. ncbi Mechanism of action of Escherichia coli endonuclease III
    Y W Kow
    Department of Microbiology and Immunology, New York Medical College, Valhalla 10595
    Biochemistry 26:8200-6. 1987
    ....
  10. pmc Importance of thiols in the repair mechanisms of DNA containing AP (apurinic or apyrimidinic) sites
    V Bailly
    Department of Biochemistry, Faculty of Sciences, University of Liege, Belgium
    Nucleic Acids Res 16:9489-96. 1988
    ..In living cells, thiols might influence the pathways followed by the repair processes of AP site-containing DNA...
  11. ncbi Chemical rearrangement and repair pathways of 1,N6-ethenoadenine
    Elzbieta Speina
    Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawinskiego 5A, 02 106 Warsaw, Poland
    Mutat Res 531:205-17. 2003
    ..coli (Nth) and Saccharomyces cerevisiae (Ntg2)...
  12. ncbi In vitro studies on DNA-photosensitization by different drug stereoisomers
    V Lhiaubet-Vallet
    Departamento de Quimica, Instituto de Tecnología Química UPV CSIC Universidad Politécnica de Valencia, Camino de Vera s n, E 46071 Valencia, Spain
    Toxicol In Vitro 17:651-6. 2003
    ..In the case of carprofen the (S) isomer appears to be somewhat less active than its (R) enantiomer. However, due to the small differences found, the possible stereoselectivity has to be confirmed by future studies...
  13. ncbi An evolutionary analysis of the helix-hairpin-helix superfamily of DNA repair glycosylases
    Dee R Denver
    Department of Biology, Indiana University, USA
    Mol Biol Evol 20:1603-11. 2003
    ..glycosylase; MpgII: N-methylpurine glycosylase II; MutY/Mig: A/G-specific adenine glycosylase/mismatch glycosylase; Nth: endonuclease III; OggI: 8-oxoguanine glycosylase I; and OggII: 8-oxoguanine glycosylase II) are identified through ..
  14. pmc Mutants of the base excision repair glycosylase, endonuclease III: DNA charge transport as a first step in lesion detection
    Christine A Romano
    Division of Chemistry and Chemical Engineering, California Institute of Technolog, Pasadena, California 91125, USA
    Biochemistry 50:6133-45. 2011
    ..These results demonstrate a link between the ability of the repair protein to carry out DNA CT and its ability to relocalize near lesions, thus pointing to DNA CT as a key first step in the detection of base damage in the genome...
  15. ncbi Chloroethylnitrosourea-derived ethano cytosine and adenine adducts are substrates for Escherichia coli glycosylases excising analogous etheno adducts
    Anton B Guliaev
    Life Sciences Division, Lawrence Berkeley National Laboratory, University of California, Berkeley, CA 94720, USA
    DNA Repair (Amst) 3:1311-21. 2004
    ..However, also as shown by MD, the stacking interaction between the EC base and Phe 30 in the Mug active site is reduced as compared to the epsilonC base, which could account for the lower EC activity observed in this study...
  16. ncbi Participation of BER and NER pathways in the repair of DNA lesions induced at low N-nitrosodiethylamine concentrations
    Claudia Alessandra Fortes Aiub
    Departamento de Biofisica e Biometria, Instituto de Biologia Roberto Alcantara Gomes, Universidade do Estado do Rio de Janeiro, Av 28 de Setembro, 87 fds, 4 andar, 20551 030 Rio de Janeiro, Brazil
    Toxicol Lett 154:133-42. 2004
    ..through cell survival, in different single and double Escherichia coli DNA repair mutants (uvrA, uvrB, uvrC, fpg, nth, xthA, fpg/nth, uvrA/fpg, fpg/xthA, mutY, and fpg/mutY), using pre-incubation periods of 90 min...
  17. ncbi 8-OxoA inhibits the incision of an AP site by the DNA glycosylases Fpg, Nth and the AP endonuclease HAP1
    Martine E Lomax
    Medical Research Council, Radiation and Genome Stability Unit, Harwell, Didcot, Oxfordshire, OX11 0RD, United Kingdom
    Radiat Res 163:79-84. 2005
    ..The influence 8-oxoA has on the incision of the AP site by the E. coli glycosylases Fpg and Nth protein and the human AP endonuclease HAP1 was assessed...
  18. ncbi E. coli BW535, a triple mutant for the DNA repair genes xth, nth, and nfo, chronically induces the SOS response
    Celina Janion
    Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland
    Environ Mol Mutagen 41:237-42. 2003
    A strong chronic induction of the SOS response system occurs in E. coli BW535, a strain defective in nth, nfo and xth genes, and hence severely deficient in the repair of abasic sites in DNA...
  19. ncbi DNA-bound redox activity of DNA repair glycosylases containing [4Fe-4S] clusters
    Amie K Boal
    Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, California 91125, USA
    Biochemistry 44:8397-407. 2005
    ..This redox activation furthermore establishes a functional role for the ubiquitous [4Fe-4S] clusters in DNA repair enzymes that involves redox chemistry and provides a means to consider DNA-mediated signaling within the cell...
  20. pmc Excision of formamidopyrimidine lesions by endonucleases III and VIII is not a major DNA repair pathway in Escherichia coli
    Carissa J Wiederholt
    Department of Chemistry, Johns Hopkins University 3400 North Charles Street, Baltimore, MD 21218, USA
    Nucleic Acids Res 33:3331-8. 2005
    ..O. Blaisdell, Z. Hatahet and S. S. Wallace (1999) J. Bacteriol., 181, 6396-6402], these results also suggest that Endo III and Endo VIII do not protect E.coli against possible mutations attributable to formamidopyrimidine lesions...
  21. ncbi Increased sensitivity to sparsely ionizing radiation due to excessive base excision in clustered DNA damage sites in Escherichia coli
    P W Chang
    Laboratory of Radiation Biology, Graduate School of Science, Kyoto University, Japan
    Int J Radiat Biol 81:115-23. 2005
    ..In order to clarify the cellular processing and repair mechanisms for radiation-induced clustered DNA damage, we examined the correlation between the levels of DNA glycosylases and the sensitivity to ionizing radiation in Escherichia coli...
  22. ncbi Engineering functional changes in Escherichia coli endonuclease III based on phylogenetic and structural analyses
    Takashi Watanabe
    Department of Microbiology and Molecular Genetics, The University of Vermont, Burlington, Vermont 05405, USA
    J Biol Chem 280:34378-84. 2005
    ..These three amino acids are highly conserved among Nth orthologs, although not among homologous glycosylases, such as MutY, that have different base specificities and no ..
  23. pmc Synthesis and characterization of oligonucleotides containing 2'-fluorinated thymidine glycol as inhibitors of the endonuclease III reaction
    Yusuke Doi
    Division of Chemistry, Graduate School of Engineering Science, Osaka University, 1 3 Machikaneyama, Toyonaka, Osaka 560 8531, Japan
    Nucleic Acids Res 34:1540-51. 2006
    ..The 5S isomer was found to form an enzyme-DNA complex, but the incision was inhibited probably by the fluorine-induced stabilization of the glycosidic bond...
  24. pmc DNA repair glycosylases with a [4Fe-4S] cluster: a redox cofactor for DNA-mediated charge transport?
    Amie K Boal
    Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, CA 91125, USA
    J Inorg Biochem 101:1913-21. 2007
    ....
  25. ncbi Base excision repair enzyme endonuclease III suppresses mutagenesis caused by 8-hydroxy-dGTP
    Tetsuya Suzuki
    Faculty of Pharmaceutical Sciences, Hokkaido University, Kita 12, Nishi 6, Kita ku, Sapporo 060 0812, Japan
    DNA Repair (Amst) 7:88-94. 2008
    ..8-OH-dGTP) and 2-hydroxy-dATP were introduced into Escherichia coli strains deficient in endonucleases III (Nth) and VIII (Nei) and MutY, and mutations in the chromosomal rpoB gene were analyzed...
  26. ncbi Base excision repair system suppresses mutagenesis caused by 8-hydroxy-dGTP in Escherichia coli
    Tetsuya Suzuki
    Faculty of Pharmaceutical Sciences, Hokkaido University, Kita 12, Nishi 6, Kita ku, Sapporo 060 0812, Japan
    Nucleic Acids Symp Ser (Oxf) . 2007
    ..pool, 8-hydroxy-dGTP (8-OH-dGTP) was introduced into Escherichia coli strains deficient in endonucleases III (Nth) and VIII (Nei), and MutY, and mutations in the chromosomal rpoB gene were analyzed...
  27. doi Human Nei-like protein NEIL3 has AP lyase activity specific for single-stranded DNA and confers oxidative stress resistance in Escherichia coli mutant
    Masashi Takao
    Department of Molecular Genetics, Institute of Development, Aging and Cancer, Tohoku University, Aoba ku, Sendai, Japan
    Genes Cells 14:261-70. 2009
    ..Surprisingly, NEIL3 partially rescues an E. coli nth nei mutant from hydrogen peroxide sensitivity...
  28. pmc IscA/SufA paralogues are required for the [4Fe-4S] cluster assembly in enzymes of multiple physiological pathways in Escherichia coli under aerobic growth conditions
    Guoqiang Tan
    Department of Biological Sciences, Louisiana State University, Baton Rouge, LA 70803, U S A
    Biochem J 420:463-72. 2009
    ..coli under aerobic conditions...
  29. pmc Redox signaling between DNA repair proteins for efficient lesion detection
    Amie K Boal
    Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, CA 91125, USA
    Proc Natl Acad Sci U S A 106:15237-42. 2009
    ..These results illustrate how repair proteins might efficiently locate DNA lesions and point to a biological role for DNA-mediated CT within the cell...
  30. doi Fluorescence detection of the endonuclease III reaction using modified oligonucleotides
    Naoyuki Matsumoto
    Division of Chemistry, Graduate School of Engineering Science, Osaka University, Toyonaka, Osaka 560 8531, Japan
    Nucleic Acids Symp Ser (Oxf) . 2009
    ..The substrate specificity was shown using Escherichia coli and human enzymes...
  31. doi Lethal and mutagenic properties of MMS-generated DNA lesions in Escherichia coli cells deficient in BER and AlkB-directed DNA repair
    Anna Sikora
    Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawinskiego 5A, 02 106 Warszawa, Poland
    Mutagenesis 25:139-47. 2010
    ..In the xth nth nfo strain additionally mutated in alkB gene, all these effects were extreme and led to 'error catastrophe', ..
  32. ncbi Excision of oxidative cytosine modifications from gamma-irradiated DNA by Escherichia coli endonuclease III and human whole-cell extracts
    J R Wagner
    Centre de recherche en gérontologie et gériatrie, Hôpital d Youville, Sherbrooke, Quebec, Canada
    Anal Biochem 233:76-86. 1996
    ..In identical samples, the amount of product 2 was reduced by 45.0 +/- 2.6% (225 from 500 fmol per microgram of DNA) and that of product 4 by 7.0 +/- 3.1% (42 from 600 fmol per microgram of DNA) as measured by HPLC/EC analysis...
  33. ncbi Atomic structure of the DNA repair [4Fe-4S] enzyme endonuclease III
    C F Kuo
    Department of Molecular Biology, Scripps Research Institute, La Jolla, CA 92037
    Science 258:434-40. 1992
    ..Overall, the structure reveals an unusual fold and a new biological function for [4Fe-4S] clusters and provides a structural basis for studying recognition of damaged DNA and the N-glycosylase and apurinic/apyrimidinic-lyase mechanisms...
  34. pmc Escherichia coli endonuclease III is not an endonuclease but a beta-elimination catalyst
    V Bailly
    Biochem J 242:565-72. 1987
    ..The cleavage might be the result of a beta-elimination analogous to the one produced by an alkaline pH or Lys-Trp-Lys. Thus it would seem that E. coli 'endonuclease III' is, after all, not an endonuclease...
  35. pmc The mechanisms of action of E. coli endonuclease III and T4 UV endonuclease (endonuclease V) at AP sites
    J Kim
    Department of Biochemistry, University of California, Berkeley 94720
    Nucleic Acids Res 16:1135-41. 1988
    ..Whereas these enzymes use a lyase-like rather than a hydrolytic mechanism, they nonetheless catalyze phosphodiester bond cleavage. We suggest that the term endonuclease can be properly applied to them...
  36. ncbi Purification and characterization of Escherichia coli endonuclease III from the cloned nth gene
    H Asahara
    Department of Biological Sciences, State University of New York, Albany 12222
    Biochemistry 28:4444-9. 1989
    The gene which codes for endonuclease III of Escherichia coli has been sequenced. The nth gene was previously subcloned and defined as the gene which led to overproduction of endonuclease III when present on a multicopy plasmid and which ..
  37. pmc Endonuclease III (nth) mutants of Escherichia coli
    R P Cunningham
    Proc Natl Acad Sci U S A 82:474-8. 1985
    ..An insertion mutation was produced by cloning the kanamycin-resistance gene of Tn5 into the plasmid copy of the nth gene...
  38. pmc Genetic mapping of nth, a gene affecting endonuclease III (thymine glycol-DNA glycosylase) in Escherichia coli K-12
    B Weiss
    J Bacteriol 162:607-10. 1985
    The nth gene of Escherichia coli affects the production of endonuclease III, a glycosylase-endonuclease that attacks DNA damaged by oxidizing agents or by ionizing radiation. An nth insertion mutant and a deletion mutant were studied...
  39. pmc Novel DNA binding motifs in the DNA repair enzyme endonuclease III crystal structure
    M M Thayer
    Scripps Research Institute, Department of Molecular Biology MB4, La Jolla, CA 92037, USA
    EMBO J 14:4108-20. 1995
    ..The identification of HhH and FCL sequence patterns in other DNA binding proteins suggests that these motifs may be a recurrent structural theme for DNA binding proteins...
  40. ncbi Structure and function of Escherichia coli endonuclease III
    R P Cunningham
    Department of Biological Sciences, State University of New York, Albany 12222
    Ann N Y Acad Sci 726:215-22. 1994
  41. ncbi Properties and biological functions of the NTH and FPG proteins of Escherichia coli: two DNA glycosylases that repair oxidative damage in DNA
    S Boiteux
    LA 147 CNRS, U140 INSERM, Institut Gustave Roussy, Villejuif, France
    J Photochem Photobiol B 19:87-96. 1993
    ..The first step in this DNA repair pathway is catalysed either by the NTH protein which excises oxidized pyrimidines or by the FPG protein which excises oxidized purines...
  42. ncbi Role of DNA repair enzymes in the cellular resistance to oxidative stress
    J Laval
    Groupe de Réparation des lésions radio et chimio induites, URA 147 CNRS, Institut Gustave Roussy, Villejuif, France
    Pathol Biol (Paris) 44:14-24. 1996
    ..Efficient DNA repair mechanisms remove these oxidized bases. In Escherichia coli cells, endonuclease III (NTH protein) and endonuclease VIII (NEI protein) excise many oxidized pyrimidines, whereas the FPG protein (..
  43. ncbi DNA base damage induced by ionizing radiation recognized by Escherichia coli UvrABC nuclease but not Nth or Fpg proteins
    T Roldan-Arjona
    Imperial Cancer Research Fund, Clare Hall Laboratories, South Mimms Hertfordshire, United Kingdom
    Mol Carcinog 16:188-96. 1996
    ..major known lesions are repaired by two well-characterized DNA glycosylases of Escherichia coli, endonuclease III (Nth) and formamidopyrimidine-DNA glycosylase (Fpg), which have associated AP lyase activities...
  44. ncbi Cross-linking of 2-deoxyribonolactone and its beta-elimination product by base excision repair enzymes
    Kelly M Kroeger
    Department of Chemistry, Johns Hopkins University, 3400 North Charles Street, Baltimore, Maryland 21218, USA
    Biochemistry 42:2449-55. 2003
    ....
  45. pmc Characterization of endonuclease III (nth) and endonuclease VIII (nei) mutants of Escherichia coli K-12
    Y Saito
    Biological Institute, Graduate School of Science, Tohoku University, Sendai, Japan
    J Bacteriol 179:3783-5. 1997
    The nth and nei genes of Escherichia coli affect the production of endonuclease III and endonuclease VIII, respectively, glycosylases/apurinic lyases that attack DNA damaged by oxidizing agents...
  46. pmc Multiply damaged sites in DNA: interactions with Escherichia coli endonucleases III and VIII
    L Harrison
    Department of Microbiology and Molecular Genetics, Markey Center for Molecular Genetics, University of Vermont, Burlington, VT 05405, USA
    Nucleic Acids Res 26:932-41. 1998
    ....
  47. ncbi Enzymatic processing of uracil glycol, a major oxidative product of DNA cytosine
    A A Purmal
    Department of Microbiology and Molecular Genetics, The Markey Center for Molecular Genetics, University of Vermont, Burlington, Vermont 05405, USA
    J Biol Chem 273:10026-35. 1998
    ..The steady state kinetic parameters indicated that Ug was a better substrate for endo III and formamidopyrimidine DNA glycosylase than Tg; for endonuclease VIII, however, Tg was a better substrate...
  48. ncbi The ring fragmentation product of thymidine C5-hydrate when present in DNA is repaired by the Escherichia coli Fpg and Nth proteins
    J Jurado
    Groupe Reparation des lesions Radio et Chimio Induites, UMR 1772 CNRS, Institut Gustave Roussy, Villejuif, France
    Biochemistry 37:7757-63. 1998
    ..alphaRT is a substrate for two purified enzymes from Escherichia coli involved in the repair of oxidized bases: the Nth and the Fpg proteins. The Fpg protein removes the alphaRT lesion more efficiently than the Nth protein...
  49. ncbi Excision of 5,6-dihydroxy-5,6-dihydrothymine, 5,6-dihydrothymine, and 5-hydroxycytosine from defined sequence oligonucleotides by Escherichia coli endonuclease III and Fpg proteins: kinetic and mechanistic aspects
    C D'Ham
    Laboratoire Lesions des Acides Nucleiques, Service de Chimie Inorganique et Biologique, Departement de Recherche Fondamentale sur la Matiere Condensee, CEA Grenoble, France
    Biochemistry 38:3335-44. 1999
    ....
  50. ncbi Radiation-induced DNA base damage detected in individual aerobic and hypoxic cells with endonuclease III and formamidopyrimidine-glycosylase
    J P Banath
    Medical Biophysics Department, British Columbia Cancer Research Centre, Vancouver, Canada
    Radiat Res 151:550-8. 1999
    ..0, and (2) the presence of direct DNA strand breaks (>2000-4000 per cell) prevents accurate detection of base damage measured as enzyme-sensitive sites with the alkaline comet method...
  51. ncbi Endonuclease III and endonuclease IV protect Escherichia coli from the lethal and mutagenic effects of near-UV irradiation
    D M Serafini
    Department of Biology, McMaster University, Hamilton, ON, Canada
    Can J Microbiol 45:632-7. 1999
    ..isogenic strains deficient in one or more of exonuclease III (xthA), endonuclease IV (nfo), and endonuclease III (nth) were exposed to increasing levels of far-UV and near-UV...
  52. pmc The genes encoding endonuclease VIII and endonuclease III in Escherichia coli are transcribed as the terminal genes in operons
    C M Gifford
    Department of Microbiology and Molecular Genetics, The Markey Center for Molecular Genetics, The University of Vermont, Stafford Hall, Burlington, VT 05405 0068, USA
    Nucleic Acids Res 28:762-9. 2000
    ..The genes encoding these proteins, nei and nth, are both co-transcribed as the terminal genes in operons...
  53. ncbi Clustered DNA damage, influence on damage excision by XRS5 nuclear extracts and Escherichia coli Nth and Fpg proteins
    M H David-Cordonnier
    Medical Research Council, Radiation and Genome Stability Unit, Harwell, Didcot, Oxon, OX11 0RD, United Kingdom
    J Biol Chem 275:11865-73. 2000
    ..Escherichia coli Nth and Fpg and nuclear extracts from XRS5, a Chinese hamster ovary Ku-deficient cell line, have been used to study the ..
  54. pmc Multiprobe RNase protection assay analysis of mRNA levels for the Escherichia coli oxidative DNA glycosylase genes under conditions of oxidative stress
    C M Gifford
    Department of Microbiology and Molecular Genetics, The Markey Center for Molecular Genetics, The University of Vermont, Burlington, Vermont 05405 0068, USA
    J Bacteriol 182:5416-24. 2000
    ..The genes fpg, mutY, nei, and nth encode Fpg, MutY, endonuclease VIII, and endonuclease III, respectively...
  55. ncbi AlkA protein is the third Escherichia coli DNA repair protein excising a ring fragmentation product of thymine
    C V Privezentzev
    Groupe Reparation des lesions Radio et Chimio Induites, UMR 8532 CNRS, Institut Gustave Roussy, 94805 Villejuif Cedex, France
    Biochemistry 39:14263-8. 2000
    ..It was shown that alphaRT is excised by Escherichia coli Fpg and Nth proteins. Here we report that when present in DNA, alphaRT is, in addition, a substrate for the E...
  56. ncbi Increased base change mutations at G:C pairs in Escherichia coli deficient in endonuclease III and VIII
    K Tano
    Research Reactor Institute, Kyoto University, Noda, Kumatori, Sennan gun, Osaka 590 0454, Japan
    J Radiat Res 42:409-13. 2001
    ..G:C to T:A and to C:G transversions dominated in both mutants. These results suggest that endo III and endo VIII are involved in the repair of oxidative lesions of guanine...
  57. ncbi Crystallization and crystallographic characterization of the iron-sulfur-containing DNA-repair enzyme endonuclease III from Escherichia coli
    C F Kuo
    Department of Molecular Biology, Scripps Research Institute, La Jolla, CA 92037
    J Mol Biol 227:347-51. 1992
    ..The elucidation of endonuclease III structure will facilitate not only the understanding of glycosylase and lyase mechanisms but also the structure and function of this new class of iron-sulfur proteins...