nhaA

Summary

Gene Symbol: nhaA
Description: sodium-proton antiporter
Alias: ECK0020, JW0018, ant, antA
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. ncbi Model structure of the Na+/H+ exchanger 1 (NHE1): functional and clinical implications
    Meytal Landau
    Department of Biochemistry, George S Wise Faculty of Life Sciences, Tel Aviv University, 69978, Tel Aviv, Israel
    J Biol Chem 282:37854-63. 2007
  2. pmc Na+-induced transcription of nhaA, which encodes an Na+/H+ antiporter in Escherichia coli, is positively regulated by nhaR and affected by hns
    N Dover
    Division of Microbial and Molecular Ecology, Institute of Life Sciences, Hebrew University of Jerusalem, Israel
    J Bacteriol 178:6508-17. 1996
  3. ncbi Expression of sodium/proton antiporter NhaA at various pH values in Escherichia coli
    T Shijuku
    Graduate School of Pharmaceutical Sciences, Chiba University, 1 33, Yayoi cho, Inage Ku, Chiba 263 8522, Japan
    Biochim Biophys Acta 1506:212-7. 2001
  4. ncbi Chimeric Na(+)/H(+) antiporters constructed from NhaA of Helicobacter pylori and Escherichia coli: implications for domains of NhaA for pH sensing
    H Inoue
    Department of Biological Sciences, Graduate School of Science, Osaka University, Toyonaka, Osaka 560 0043, Japan
    J Biochem 129:569-76. 2001
  5. ncbi Mutation E252C increases drastically the Km value for Na+ and causes an alkaline shift of the pH dependence of NhaA Na+/H+ antiporter of Escherichia coli
    Tzvi Tzubery
    Alexander Silberman Institute of Life Sciences, Hebrew University of Jerusalem, 91904 Jerusalem, Israel
    J Biol Chem 279:3265-72. 2004
  6. ncbi The monoclonal antibody 1F6 identifies a pH-dependent conformational change in the hydrophilic NH(2) terminus of NhaA Na(+)/H(+) antiporter of Escherichia coli
    M Venturi
    Max Planck Institut fur Biophysik, Abteilung Molekulare Membranbiologie, Heinrich Hoffmann Strasse 7, D 60528 Frankfurt Main, Germany
    J Biol Chem 275:4734-42. 2000
  7. ncbi Na+ translocation by complex I (NADH:quinone oxidoreductase) of Escherichia coli
    J Steuber
    Mikrobiologisches Institut der Eidgenössischen Technischen Hochschule, ETH Zentrum, Schmelzbergstr 7, CH 8092 Zurich, Switzerland
    Mol Microbiol 35:428-34. 2000
  8. pmc Locating ligand binding and activation of a single antiporter
    Alexej Kedrov
    BIOTEC, University of Technology, Tatzberg 47, 01062 Dresden, Germany
    EMBO Rep 6:668-74. 2005
  9. pmc Alkaline pH homeostasis in bacteria: new insights
    Etana Padan
    Alexander Silberman Institute of Life Sciences, Hebrew University, Jerusalem 91904, Israel
    Biochim Biophys Acta 1717:67-88. 2005
  10. ncbi A point mutation (G338S) and its suppressor mutations affect both the pH response of the NhaA-Na+/H+ antiporter as well as the growth phenotype of Escherichia coli
    A Rimon
    Division of Microbial and Molecular Ecology, The Hebrew University of Jerusalem, 91904 Jerusalem, Israel
    J Biol Chem 273:26470-6. 1998

Research Grants

  1. Time Domain EPR Spectrometer
    ALBERT BETH; Fiscal Year: 2005
  2. Vanderbilt Summer Research Experience for Undergraduates
    ALBERT BETH; Fiscal Year: 2006
  3. Spectroscopic Studies of EGF-Receptor Interactions
    ALBERT BETH; Fiscal Year: 2006
  4. EPR STUDIES OF MEMBRANE PROTEINS
    ALBERT BETH; Fiscal Year: 2007

Scientific Experts

  • Etana Padan
  • K A Williams
  • Elena Olkhova
  • P Dibrov
  • A Rothman
  • M L Verkhovskaya
  • M Venturi
  • T Noumi
  • ALBERT BETH
  • Abraham Rimon
  • Alexej Kedrov
  • Katia Herz
  • Lena Kozachkov
  • A Rimon
  • Christine Ziegler
  • Tzvi Tzubery
  • Daniel J Muller
  • Y Gerchman
  • Carola Hunte
  • Werner Kuhlbrandt
  • Matthias Appel
  • Livnat Galili
  • Manish Dwivedi
  • Hartmut Michel
  • Assaf Friedler
  • Chiara Lee
  • H Inoue
  • Emanuela Screpanti
  • Florian Cymer
  • N Dover
  • Michal Maes
  • E Džafić
  • Isaiah T Arkin
  • Gunnar Jeschke
  • Meytal Landau
  • Minghui Xiang
  • R J Rowbury
  • D E Otzen
  • S Schuldiner
  • Harald Janovjak
  • O Carmel
  • D Zuber
  • Daniel Hilger
  • Y Olami
  • Shahar Sukenik
  • H Kobayashi
  • E Pinner
  • Mark S P Sansom
  • Povilas Uzdavinys
  • Oliver Beckstein
  • David L Dotson
  • So Iwata
  • Christoph von Ballmoos
  • David Drew
  • Shoko Yashiro
  • Alexander D Cameron
  • H Kanazawa
  • Gunnar von Heijne
  • A W Ravna
  • Lena Kozachkov-Magrisso
  • C A Wiebe
  • T Shijuku
  • A Majernik
  • J Steuber
  • H Saito
  • C Hunte
  • T Kakegawa
  • Dilem Hizlan
  • E Screpanti
  • O Klein
  • W Mäntele
  • Kutti R Vinothkumar
  • Hella Baumann
  • T Tsuchiya
  • Sabina Muend
  • Estelle R Bennett
  • Rajini Rao
  • Nir Ben-Tal
  • Yibing Shan
  • Michael P Eastwood
  • P Sehgal
  • Ravenna Flitman-Tene
  • Brent A Gregersen
  • L W Nesgaard
  • John L Klepeis
  • Huafeng Xu
  • Mingye Feng
  • Morten Ø Jensen
  • Istvan Kolossvary
  • David E Shaw

Detail Information

Publications91

  1. ncbi Model structure of the Na+/H+ exchanger 1 (NHE1): functional and clinical implications
    Meytal Landau
    Department of Biochemistry, George S Wise Faculty of Life Sciences, Tel Aviv University, 69978, Tel Aviv, Israel
    J Biol Chem 282:37854-63. 2007
    ..We also suggest a hypothetical alternating-access mechanism that involves conformational changes...
  2. pmc Na+-induced transcription of nhaA, which encodes an Na+/H+ antiporter in Escherichia coli, is positively regulated by nhaR and affected by hns
    N Dover
    Division of Microbial and Molecular Ecology, Institute of Life Sciences, Hebrew University of Jerusalem, Israel
    J Bacteriol 178:6508-17. 1996
    b>nhaA encodes an Na+/H+ antiporter in Escherichia coli which is essential for adaptation to high salinity and alkaline pH in the presence of Na+. We used Northern (RNA) analysis to measure directly the cellular levels of nhaA mRNA...
  3. ncbi Expression of sodium/proton antiporter NhaA at various pH values in Escherichia coli
    T Shijuku
    Graduate School of Pharmaceutical Sciences, Chiba University, 1 33, Yayoi cho, Inage Ku, Chiba 263 8522, Japan
    Biochim Biophys Acta 1506:212-7. 2001
    It was reported that NhaA, one of sodium/proton antiporters in Escherichia coli, was expressed at alkaline pH [J. Biol. Chem. 266 (1991) 21753]...
  4. ncbi Chimeric Na(+)/H(+) antiporters constructed from NhaA of Helicobacter pylori and Escherichia coli: implications for domains of NhaA for pH sensing
    H Inoue
    Department of Biological Sciences, Graduate School of Science, Osaka University, Toyonaka, Osaka 560 0043, Japan
    J Biochem 129:569-76. 2001
    In order to delineate regions which play a role in the regulation of Na(+)/H(+) antiporter NhaA activity by pH, we analyzed the antiporter activities of various chimeric mutants constructed from specific portions of NhaA from Escherichia ..
  5. ncbi Mutation E252C increases drastically the Km value for Na+ and causes an alkaline shift of the pH dependence of NhaA Na+/H+ antiporter of Escherichia coli
    Tzvi Tzubery
    Alexander Silberman Institute of Life Sciences, Hebrew University of Jerusalem, 91904 Jerusalem, Israel
    J Biol Chem 279:3265-72. 2004
    A single Cys replacement of Glu at position 252 (E252C) in loop VIII-IX of NhaA increases drastically the Km for Na(+) (50-fold) of the Na(+)/H(+) antiporter activity of NhaA and shifts the pH dependence of NhaA activity, by one pH unit, ..
  6. ncbi The monoclonal antibody 1F6 identifies a pH-dependent conformational change in the hydrophilic NH(2) terminus of NhaA Na(+)/H(+) antiporter of Escherichia coli
    M Venturi
    Max Planck Institut fur Biophysik, Abteilung Molekulare Membranbiologie, Heinrich Hoffmann Strasse 7, D 60528 Frankfurt Main, Germany
    J Biol Chem 275:4734-42. 2000
    One of the most interesting properties of the NhaA Na(+)/H(+) antiporter of Escherichia coli is the strong regulation of its activity by pH...
  7. ncbi Na+ translocation by complex I (NADH:quinone oxidoreductase) of Escherichia coli
    J Steuber
    Mikrobiologisches Institut der Eidgenössischen Technischen Hochschule, ETH Zentrum, Schmelzbergstr 7, CH 8092 Zurich, Switzerland
    Mol Microbiol 35:428-34. 2000
    ..Our study object was the Escherichia coli mutant EP432, which lacks the Na+/H+ antiporter genes nhaA and nhaB and is therefore unable to grow on LB medium at elevated Na+ concentrations...
  8. pmc Locating ligand binding and activation of a single antiporter
    Alexej Kedrov
    BIOTEC, University of Technology, Tatzberg 47, 01062 Dresden, Germany
    EMBO Rep 6:668-74. 2005
    Single-molecule force spectroscopy was applied to unfold individual Na(+)/H(+) antiporters NhaA from membrane patches...
  9. pmc Alkaline pH homeostasis in bacteria: new insights
    Etana Padan
    Alexander Silberman Institute of Life Sciences, Hebrew University, Jerusalem 91904, Israel
    Biochim Biophys Acta 1717:67-88. 2005
    ..New insights into the pH-dependent Na(+)/H(+) antiporter NhaA that plays an important role in Escherichia coli have recently emerged from the determination of the structure of ..
  10. ncbi A point mutation (G338S) and its suppressor mutations affect both the pH response of the NhaA-Na+/H+ antiporter as well as the growth phenotype of Escherichia coli
    A Rimon
    Division of Microbial and Molecular Ecology, The Hebrew University of Jerusalem, 91904 Jerusalem, Israel
    J Biol Chem 273:26470-6. 1998
    pH controls the activity of the NhaA Na+/H+ antiporter of Escherichia coli. In the present work we show that replacement of glycine 338 of NhaA with serine (G338S) alleviates the pH control of the antiporter...
  11. pmc The Na+-specific interaction between the LysR-type regulator, NhaR, and the nhaA gene encoding the Na+/H+ antiporter of Escherichia coli
    O Carmel
    Division of Microbial and Molecular Ecology, The Institute of Life Sciences, The Hebrew University of Jerusalem, 91904 Jerusalem, Israel
    EMBO J 16:5922-9. 1997
    ..purified NhaR and a highly purified His-tagged NhaR derivative to identify the cis-regulatory sequences of nhaA recognized by NhaR and to study the specific effect of Na+ on this interaction...
  12. ncbi Crucial steps in the structure determination of the Na+/H+ antiporter NhaA in its native conformation
    Emanuela Screpanti
    Department of Molecular Membrane Biology, Max Planck Institute of Biophysics, Max von Laue Str 3, D 60438 Frankfurt, Germany
    J Mol Biol 362:192-202. 2006
    ..Recently, the first structure of this family of secondary transporters, namely of NhaA from Escherichia coli, revealed a novel fold and elucidated the molecular basis for the mechanism of transport and ..
  13. ncbi Exposure of Escherichia coli to acid habituation conditions sensitizes it to alkaline stress
    R J Rowbury
    Department of Biology Darwin, University College London, UK
    Lett Appl Microbiol 22:57-61. 1996
    ..5 plus DNA gyrase inhibitors and was markedly reduced by himA, himD, hns, ompC and nhaA lesions. A tonB deletion mutant showed alkali sensitivity at pHo 7.0...
  14. pmc Physiological role of the chaA gene in sodium and calcium circulations at a high pH in Escherichia coli
    T Ohyama
    Faculty of Pharmaceutical Sciences, Chiba University, Japan
    J Bacteriol 176:4311-5. 1994
    ..The resultant mutant, TO112, deficient in both nhaA and chaA, was unable to grow at pH 8.5 in medium containing 0...
  15. pmc The Ec-NhaA antiporter switches from antagonistic to synergistic antiport upon a single point mutation
    Manish Dwivedi
    Department of Biological Chemistry, Alexander Silberman Institute of Life Sciences, Givat Ram, Jerusalem 91904, Israel
    Sci Rep 6:23339. 2016
    The Na(+), Li(+)/H(+) antiporter of Escherichia coli (Ec-NhaA) maintains pH, Na(+) homeostasis in enterobacteria...
  16. pmc Transmembrane segment II of NhaA Na+/H+ antiporter lines the cation passage, and Asp65 is critical for pH activation of the antiporter
    Katia Herz
    Department of Biological Chemistry, Alexander Silberman Institute of Life Sciences, Hebrew University, 91904 Jerusalem, Israel
    J Biol Chem 285:2211-20. 2010
    The crystal structure of Escherichia coli NhaA determined at pH 4 has provided insights into the mechanism of activity of a pH-regulated Na+/H+ antiporter. However, because NhaA is activated at physiological pH (pH 5.5-8...
  17. pmc Sodium chloride induces an NhaA/NhaR-independent acid sensitivity at neutral external pH in Escherichia coli
    R J Rowbury
    Department of Biology Darwin, University College London, United Kingdom
    Appl Environ Microbiol 60:1630-4. 1994
    ..Sensitization was not prevented by deletion of the nhaA, nhaR, or nhaB gene...
  18. ncbi Sequencing of the gene ant which affects the Na+/H+ antiporter activity in Escherichia coli
    R Karpel
    Department of Molecular and Microbial Ecology, Hadassah Medical School, Hebrew University, Jerusalem, Israel
    J Biol Chem 263:10408-14. 1988
    ..58 kilobase pairs) which contains the ant gene. Thus, transformants of all plasmid constructs containing this segment exhibit Antup phenotype, i.e...
  19. doi Conformations of NhaA, the Na/H exchanger from Escherichia coli, in the pH-activated and ion-translocating states
    Matthias Appel
    Department of Structural Biology, Max Planck Institute of Biophysics, Frankfurt am Main, Germany
    J Mol Biol 386:351-65. 2009
    b>NhaA, the main sodium-proton exchanger in the inner membrane of Escherichia coli, regulates the cytosolic concentrations of H and Na. It is inactive at acidic pH, becomes active between pH 6 and pH 7, and reaches maximum activity at pH 8...
  20. doi Beta-sheet-dependent dimerization is essential for the stability of NhaA Na+/H+ antiporter
    Katia Herz
    Department of Biological Chemistry, Alexander Silberman Institute of Life Sciences, Hebrew University of Jerusalem, 91904 Jerusalem, Israel
    J Biol Chem 284:6337-47. 2009
    A structural model of the NhaA dimer showed that a beta-hairpin of each monomer combines to form a beta-sheet at the periplasmic side of the membrane...
  21. pmc Crystal structure of the sodium-proton antiporter NhaA dimer and new mechanistic insights
    Chiara Lee
    Division of Molecular Biosciences, Imperial College London, London SW7 2AZ, England, UK
    J Gen Physiol 144:529-44. 2014
    ..Here, we report a crystal form of the prototypical sodium-proton antiporter NhaA from Escherichia coli in which the protein is seen as a dimer...
  22. pmc Cotranslational folding of membrane proteins probed by arrest-peptide-mediated force measurements
    Florian Cymer
    Center for Biomembrane Research, Department of Biochemistry and Biophysics, Stockholm University, SE 106 91 Stockholm, Sweden
    Proc Natl Acad Sci U S A 110:14640-5. 2013
    ..Our findings pinpoint the earliest steps of tertiary structure formation and open up possibilities to study the cotranslational folding of polytopic membrane proteins. ..
  23. pmc Revealing the ligand binding site of NhaA Na+/H+ antiporter and its pH dependence
    Michal Maes
    Institute of Chemistry, Alexander Silberman Institute of Life Sciences, Faculty of Sciences, Hebrew University of Jerusalem, Safra Campus, Givat Ram, Jerusalem 91904, Israel
    J Biol Chem 287:38150-7. 2012
    ..In most cases, their activity is tightly pH-regulated. NhaA, the main antiporter of Escherichia coli, has homologues in all biological kingdoms...
  24. ncbi Unraveling functional and structural interactions between transmembrane domains IV and XI of NhaA Na+/H+ antiporter of Escherichia coli
    Livnat Galili
    Alexander Silberman Institute of Life Sciences, Hebrew University of Jerusalem, Jerusalem 91904, Israel
    J Biol Chem 279:23104-13. 2004
    A functionally important, interface domain between transmembrane segments (TMSs) IV and XI of the NhaA Na+/H+ antiporter of Escherichia coli has been unraveled...
  25. ncbi Monoclonal antibodies for the structural analysis of the Na+/H+ antiporter NhaA from Escherichia coli
    Miro Venturi
    Structural Biology Section, Vaccine Research Center National Institutes of Health, Bethesda, MD 20892, USA
    Biochim Biophys Acta 1610:46-50. 2003
    ..In this review, we focus on the methods used to generate monoclonal antibodies against the NhaA antiporter from Escherichia coli as a paradigm of secondary transporters...
  26. pmc Site-directed tryptophan fluorescence reveals two essential conformational changes in the Na+/H+ antiporter NhaA
    Lena Kozachkov
    Department of Biological Chemistry, Alexander Silberman Institute of Life Sciences, Hebrew University of Jerusalem, Jerusalem 91904, Israel
    Proc Natl Acad Sci U S A 108:15769-74. 2011
    b>NhaA, a Na(+)/H(+) antiporter critical for pH and Na(+) homeostasis in Escherichia coli, as well as other enterobacteria and possibly Homo sapiens, was modified for fluorescence spectroscopy by constructing a functional Trp-less NhaA ..
  27. ncbi NhaA of Escherichia coli, as a model of a pH-regulated Na+/H+antiporter
    E Padan
    Division of Microbial and Molecular Ecology, Alexander Silberman Institute of Life Sciences, Hebrew University of Jerusalem, 91904 Jerusalem, Israel
    Biochim Biophys Acta 1658:2-13. 2004
    ..The pH regulation of NhaA, the Escherichia coli Na(+)/H(+) antiporter (EcNhaA), as of other, both eukaryotic and prokaryotic Na(+)/H(+) ..
  28. ncbi 2-Aminoperimidine, a specific inhibitor of bacterial NhaA Na(+)/H(+) antiporters
    Pavel Dibrov
    Department of Microbiology, University of Manitoba, Winnipeg, Man, Canada R3T 2N2
    FEBS Lett 579:373-8. 2005
    ..Most prokaryotic antiporters, including the major NhaA family of enterobacteria, are resistant to these compounds...
  29. pmc Assessing oligomerization of membrane proteins by four-pulse DEER: pH-dependent dimerization of NhaA Na+/H+ antiporter of E. coli
    Daniel Hilger
    Ludwig Maximilians Universitat Munchen, Department Biologie I, Germany
    Biophys J 89:1328-38. 2005
    The pH dependence of the structure of the main Na(+)/H(+) antiporter NhaA of Escherichia coli is studied by continuous-wave (CW) and pulse electron paramagnetic resonance (EPR) techniques on singly spin-labeled mutants...
  30. ncbi Proximity of cytoplasmic and periplasmic loops in NhaA Na+/H+ antiporter of Escherichia coli as determined by site-directed thiol cross-linking
    A Rimon
    Alexander Silberman Institute of Life Sciences, Hebrew University of Jerusalem, Israel
    Biochemistry 41:14897-905. 2002
    The unique trypsin cleavable site of NhaA, the Na(+)/H(+) antiporter of Escherichia coli, was exploited to detect a change in mobility of cross-linked products of NhaA by polyacrylamide gel electrophoresis...
  31. ncbi Structure of a Na+/H+ antiporter and insights into mechanism of action and regulation by pH
    Carola Hunte
    Department of Molecular Membrane Biology, Max Planck Institute of Biophysics, Max von Laue Str 3, D 60438 Frankfurt, Germany
    Nature 435:1197-202. 2005
    ..Their activity is tightly controlled by pH. Here we present the crystal structure of pH-downregulated NhaA, the main antiporter of Escherichia coli and many enterobacteria...
  32. ncbi Kinetics of charge translocation in the passive downhill uptake mode of the Na+/H+ antiporter NhaA of Escherichia coli
    D Zuber
    Max Planck Institut fur Biophysik, Max von Laue Strasse 3, D 60438 Frankfurt Main, Germany
    Biochim Biophys Acta 1709:240-50. 2005
    The Na+/H+ antiporter NhaA is the main Na+ extrusion system in E. coli...
  33. ncbi Trans membrane domain IV is involved in ion transport activity and pH regulation of the NhaA-Na(+)/H(+) antiporter of Escherichia coli
    Livnat Galili
    Alexander Silberman Institute of Life Sciences, Hebrew University of Jerusalem, 91904 Jerusalem, Israel
    Biochemistry 41:609-17. 2002
    We have previously shown that the activity of NhaA is regulated by pH and found mutations that affect dramatically the pH dependence of the rate but not the K(m) (for Na(+) and Li(+)) of NhaA...
  34. ncbi Observing folding pathways and kinetics of a single sodium-proton antiporter from Escherichia coli
    Alexej Kedrov
    Center of Biotechnology, University of Technology, 01307 Dresden, Germany
    J Mol Biol 355:2-8. 2006
    ..established single-molecule force spectroscopy to observe directly the stepwise folding of the Na+/H+ antiporter NhaA from Escherichia coli in vitro...
  35. doi NhaA crystal structure: functional-structural insights
    Etana Padan
    Department of Biological Chemistry, Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, 91904 Jerusalem, Israel
    J Exp Biol 212:1593-603. 2009
    ..Many Na(+)/H(+) antiporters are tightly regulated by pH. Escherichia coli NhaA Na(+)/H(+) antiporter, a prototype pH-regulated antiporter, exchanges 2 H(+) for 1 Na(+) (or Li(+))...
  36. pmc Multiconformation continuum electrostatics analysis of the NhaA Na+/H+ antiporter of Escherichia coli with functional implications
    Elena Olkhova
    Department of Molecular Membrane Biology, Max Planck Institute of Biophysics, Max von Laue Strasse 3, D 60438 Frankfurt am Main, Germany
    Proc Natl Acad Sci U S A 103:2629-34. 2006
    ..continuum electrostatics method to investigate the protonation states of residues in the sodium proton exchanger NhaA from Escherichia coli, the structure of which has been determined recently by x-ray crystallography...
  37. doi Combined computational and biochemical study reveals the importance of electrostatic interactions between the "pH sensor" and the cation binding site of the sodium/proton antiporter NhaA of Escherichia coli
    Elena Olkhova
    Department of Molecular Membrane Biology, Max Planck Institute of Biophysics, Frankfurt am Main, Germany
    Proteins 76:548-59. 2009
    ..The crystal structure of NhaA has provided a basis to explore the mechanism of ion exchange and its unique regulation by pH...
  38. ncbi Characterizing folding, structure, molecular interactions and ligand gated activation of single sodium/proton antiporters
    Alexej Kedrov
    BioTechnological Center, University of Technology, Tatzberg 49, 01307, Dresden, Germany
    Naunyn Schmiedebergs Arch Pharmacol 372:400-12. 2006
    Using the example of sodium/proton antiporter from Escherichia coli NhaA, we review the capabilities of single-molecule atomic force microscopy and force spectroscopy to observe structural and functional insights of a membrane protein, ..
  39. ncbi Functional and structural interactions of the transmembrane domain X of NhaA, Na+/H+ antiporter of Escherichia coli, at physiological pH
    Lena Kozachkov
    Alexander Silberman Institute of Life Sciences, Hebrew University of Jerusalem, Jerusalem 91904, Israel
    Biochemistry 46:2419-30. 2007
    The 3D structure of Escherichia coli NhaA, determined at pH 4, provided the first structural insights into the mechanism of antiport and pH regulation of a Na+/H+ antiporter. However, because NhaA is activated at physiological pH (pH 7...
  40. pmc The influence of protonation states on the dynamics of the NhaA antiporter from Escherichia coli
    Elena Olkhova
    Department of Molecular Membrane Biology, Max Planck Institute of Biophysics, D 60438 Frankfurt am Main, Germany
    Biophys J 92:3784-91. 2007
    The crystal structure of NhaA Na(+)/H(+) antiporter of Escherichia coli has provided a basis to explore the mechanism of Na(+) and H(+) exchange and its regulation by pH...
  41. ncbi Oligomerization of NhaA, the Na+/H+ antiporter of Escherichia coli in the membrane and its functional and structural consequences
    Y Gerchman
    Division of Microbial and Molecular Ecology, Institute of Life Sciences, The Hebrew University of Jerusalem, 91904 Jerusalem, Israel
    Biochemistry 40:3403-12. 2001
    Recently, a two-dimensional crystal structure of NhaA, the Na+/H+ antiporter of Escherichia coli has been obtained [Williams, K. A., Kaufer, U. G., Padan, E., Schuldiner, S. and Kühlbrandt, W. (1999) EMBO J., 18, 3558-3563]...
  42. ncbi Alternative membrane protein conformations in alcohols
    D E Otzen
    Department of Life Sciences, Aalborg University, Sohngaardsholmsvej 49, DK 9000 Aalborg, Denmark aau dk
    Biochemistry 46:4348-59. 2007
    ..protein stability and solubility, using the two detergent-solubilized alpha-helical membrane proteins DsbB and NhaA. Both proteins precipitate extensively at intermediate concentrations of alcohols, forming states with extensive (..
  43. ncbi Monomers of the NhaA Na+/H+ antiporter of Escherichia coli are fully functional yet dimers are beneficial under extreme stress conditions at alkaline pH in the presence of Na+ or Li+
    Abraham Rimon
    Department of Biological Chemistry, Alexander Silberman Institute of Life Sciences, Hebrew University of Jerusalem, 91904 Jerusalem, Israel
    J Biol Chem 282:26810-21. 2007
    b>NhaA, the Na(+)/H(+) antiporter of Escherichia coli, exists in the native membrane as a homodimer of which two monomers have been suggested to be attached by a beta-hairpin at the periplasmic side of the membrane...
  44. ncbi Mechanism of Na+/H+ antiporting
    Isaiah T Arkin
    D E Shaw Research, New York, NY 10036, USA
    Science 317:799-803. 2007
    Na+/H+ antiporters are central to cellular salt and pH homeostasis. The structure of Escherichia coli NhaA was recently determined, but its mechanisms of transport and pH regulation remain elusive...
  45. doi Flexibility and dynamics of NhaA Na+/H+-antiporter of Escherichia coli studied by Fourier transform infrared spectroscopy
    E Džafić
    Institut fur Biophysik, Johann Wolfgang Goethe Universitat, Max von Laue Strasse 1, D 60438 Frankfurt am Main, Germany
    Spectrochim Acta A Mol Biomol Spectrosc 72:102-9. 2009
    b>NhaA (41,355Da) is a Na(+)/H(+) antiporter of Escherichia coli which plays a central role in regulation of intracellular pH, cellular Na(+) content, and cell volume [E. Padan, S. Schuldiner, J. Exp. Biol. 196 (1994) 443]...
  46. pmc A human Na+/H+ antiporter sharing evolutionary origins with bacterial NhaA may be a candidate gene for essential hypertension
    Minghui Xiang
    Department of Physiology, Johns Hopkins University School of Medicine, 725 North Wolfe Street, Baltimore, MD 21205, USA
    Proc Natl Acad Sci U S A 104:18677-81. 2007
    ..The NHA (sodium hydrogen antiporter) family members share significant sequence similarity with Escherichia coli NhaA, including a conserved double aspartate motif in predicted transmembrane 5...
  47. ncbi Examining the dynamic energy landscape of an antiporter upon inhibitor binding
    Alexej Kedrov
    Biotechnology Center, University of Technology, 01307 Dresden, Germany
    J Mol Biol 375:1258-66. 2008
    ..single-molecule force spectroscopy to detect and locate interactions within the functional Na(+)/H(+) antiporter NhaA from Escherichia coli...
  48. pmc Structure-based functional study reveals multiple roles of transmembrane segment IX and loop VIII-IX in NhaA Na+/H+ antiporter of Escherichia coli at physiological pH
    Tzvi Tzubery
    Department of Biological Chemistry, Alexander Silberman Institute of Life Sciences, Hebrew University of Jerusalem, 91904 Jerusalem, Israel
    J Biol Chem 283:15975-87. 2008
    The three-dimensional crystal structure of Escherichia coli NhaA determined at pH 4 provided the first structural insights into the mechanism of antiport and pH regulation of a Na(+)/H(+) antiporter...
  49. doi Epitope mapping of conformational monoclonal antibodies specific to NhaA Na+/H+ antiporter: structural and functional implications
    Abraham Rimon
    Department of Biochemistry, Alexander Silberman Institute of Life Sciences, Hebrew University of Jerusalem, 91904 Jerusalem, Israel
    J Mol Biol 379:471-81. 2008
    The recently determined crystal structure of NhaA, the Na(+)/H(+) antiporter of Escherichia coli, showed that the previously constructed series of NhaA-alkaline phosphatase (PhoA) fusions correctly predicted the topology of NhaA's 12 ..
  50. doi The enlightening encounter between structure and function in the NhaA Na+-H+ antiporter
    Etana Padan
    Department of Biological Chemistry, Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, 91904 Jerusalem, Israel
    Trends Biochem Sci 33:435-43. 2008
    ..Many Na(+)-H(+) antiporters are tightly regulated by pH. Escherichia coli NhaA, a prototype pH-regulated antiporter, exchanges 2H(+) for 1Na(+) (or Li(+))...
  51. ncbi MH1, a second-site revertant of an Escherichia coli mutant lacking Na+/H+ antiporters (delta nhaA delta nhaB), regains Na+ resistance and a capacity to excrete Na+ in a delta microH(+)-independent fashion
    M Harel-Bronstein
    Division of Microbial and Molecular Ecology, Hebrew University of Jerusalem, Israel
    J Biol Chem 270:3816-22. 1995
    The Escherichia coli mutant delta nhaA delta nhaB (EP432), which lacks the two specific Na+/H+ antiporter genes, is incapable of efficiently excreting Na+...
  52. ncbi Salt tolerance in plants and microorganisms: toxicity targets and defense responses
    R Serrano
    Instituto de Biologia Molecular y Celular de Plantas, Universidad Politecnica de Valencia CSIC, Spain
    Int Rev Cytol 165:1-52. 1996
    ..In Escherichia coli, the Na(+)-H+ antiporter encoded by the nhaA gene is essential for salt tolerance...
  53. ncbi K+-dependent Na+ transport driven by respiration in Escherichia coli cells and membrane vesicles
    M L Verkhovskaya
    Helsinki Bioenergetics Group, Institute of Biomedical Sciences, Department of Medical Chemistry, University of Helsinki, Finland
    Biochim Biophys Acta 1273:207-16. 1996
    ..coli mutant from which the genes encoding the two known electrogenic Na+/nH+ antiporters nhaA and nhaB were deleted transported Na+ as well as did vesicles from wild-type cells...
  54. ncbi Proton-sodium stoichiometry of NhaA, an electrogenic antiporter from Escherichia coli
    D Taglicht
    Division of Microbial and Molecular Ecology, Alexander Silberman Institute of Life Sciences, Hebrew University of Jerusalem, Israel
    J Biol Chem 268:5382-7. 1993
    The H+:Na+ exchange stoichiometry of NhaA, a sodium-proton antiporter coded by the nhaA gene of Escherichia coli, has been determined using purified NhaA protein reconstituted into sodium-loaded proteoliposomes...
  55. pmc Histidine-226 is part of the pH sensor of NhaA, a Na+/H+ antiporter in Escherichia coli
    Y Gerchman
    Division of Microbial and Molecular Ecology, Hebrew University of Jerusalem, Israel
    Proc Natl Acad Sci U S A 90:1212-6. 1993
    The nhaA gene of Escherichia coli, which encodes a pH-activated Na+/H+ antiporter, has been modified; six of its eight histidine codons were mutated to arginine codons by site-directed mutagenesis, yielding the mutations H254R-H257R (a ..
  56. ncbi Calcium transport mediated by NhaA, a Na+/H+ antiporter from Escherichia coli
    P A Dibrov
    Division of Microbial and Molecular Ecology, Silberman Institute of Life Sciences, Hebrew University of Jerusalem, Givat Ram, Israel
    FEBS Lett 336:530-4. 1993
    In everted membrane vesicles of E. coli strain EP432/pGM42, which has only one Na+/H+ antiporter (NhaA), external CaCl2 inhibits dissipation of the respiration-dependent delta pH in response to the addition of NaCl at pH 7...
  57. ncbi Mechanism of Na+/H+ exchange by Escherichia coli NhaA in reconstituted proteoliposomes
    P A Dibrov
    Division of Microbial and Molecular Ecology, Silberman Institute of Life Sciences, Hebrew University of Jerusalem, Givat Ram, Israel
    FEBS Lett 336:525-9. 1993
    Purified NhaA, a Na+/H+ antiporter from Escherichia coli, reconstituted into proteoliposomes was used to study partial reactions catalyzed by this protein...
  58. pmc A single amino acid substitution (Glu134-->Ala) in NhaR1 increases the inducibility by Na+ of the product of nhaA, a Na+/H+ antiporter gene in Escherichia coli
    O Carmel
    Division of Microbial and Molecular Ecology, Hebrew University of Jerusalem, Israel
    EMBO J 13:1981-9. 1994
    ..This was demonstrated by sequence analysis showing that the mutant contains a wild-type nhaA but nhaR1 instead of nhaR and by the finding that nhaR1 cloned in a plasmid confers the NhaAup phenotype...
  59. ncbi Na+/H+ antiporters, molecular devices that couple the Na+ and H+ circulation in cells
    E Padan
    Division of Microbial and Molecular Ecology, Hebrew University of Jerusalem, Israel
    J Bioenerg Biomembr 25:647-69. 1993
    ..A novel signal transduction to Na+ involving nhaR, a positive regulator, controls the expression of nhaA in E. coli. A "pH sensor" regulates the activity of Na+/H+ antiporters, both in eukaryotes and prokaryotes...
  60. ncbi Kinetic properties of NhaB, a Na+/H+ antiporter from Escherichia coli
    E Pinner
    Division of Microbial and Molecular Ecology, Alexander Silberman Institute of Life Sciences, Hebrew University of Jerusalem, Israel
    J Biol Chem 269:26274-9. 1994
    ..The significance of the existence of two antiporters with different stoichiometries, NhaA and NhaB, active in the same cell, is discussed.
  61. ncbi Topological analysis of NhaA, a Na+/H+ antiporter from Escherichia coli
    A Rothman
    Alexander Silberman Institute of Life Sciences, Hebrew University of Jerusalem, 91904 Jerusalem, Israel
    J Biol Chem 271:32288-92. 1996
    Analysis of the hydropathic profile of the amino acid sequence of NhaA, a Na+/H+ antiporter from Escherichia coli has previously suggested the existence of 11 putative transmembrane segments (Taglicht, D., Padan, E., and Schuldiner, S...
  62. ncbi Molecular physiology of the Na+/H+ antiporter in Escherichia coli
    E Padan
    Department of Molecular and Microbial Ecology, Hebrew University of Jerusalem, Israel
    J Exp Biol 196:443-56. 1994
    ..In Escherichia coli, two Na+/H+ antiporter genes, nhaA and nhaB, have been cloned...
  63. ncbi The NhaB Na+/H+ antiporter is essential for intracellular pH regulation under alkaline conditions in Escherichia coli
    T Shimamoto
    Department of Microbiology, Faculty of Pharmaceutical Sciences, Okayama University
    J Biochem 116:285-90. 1994
    ..coli. Introduction of nhaA gene into the mutant cells increased Na+/H+ antiporter activity, but did not restore the defective growth and ..
  64. ncbi Essential aspartic acid residues, Asp-133, Asp-163 and Asp-164, in the transmembrane helices of a Na+/H+ antiporter (NhaA) from Escherichia coli
    H Inoue
    Department of Biotechnology, Faculty of Engineering, Okayama University, Japan
    FEBS Lett 363:264-8. 1995
    ..Four Asp residues were located in the putative transmembrane helices of the Escherichia coli Na+/H+ antiporter, NhaA. We replaced each of these Asp residues by Asn in plasmid encoded nhaA and expressed these constructs in an E...
  65. ncbi Replacements of histidine 226 of NhaA-Na+/H+ antiporter of Escherichia coli. Cysteine (H226C) or serine (H226S) retain both normal activity and pH sensitivity, aspartate (H226D) shifts the pH profile toward basic pH, and alanine (H226A) inactivates the ca
    A Rimon
    Division of Microbial and Molecular Ecology, Hebrew University of Jerusalem, Israel
    J Biol Chem 270:26813-7. 1995
    We have previously shown that replacement of His-226 in the NhaA Na+/H+ antiporter of Escherichia coli to Arg (H226R) shifts the pH profile of the antiporter toward acidic pH and as a result of delta nhaA delta nhaB strain bearing this ..
  66. ncbi Deletion of ant in Escherichia coli reveals its function in adaptation to high salinity and an alternative Na+/H+ antiporter system(s)
    E Padan
    Department of Microbial and Molecular Ecology, Life Science Institute, Jerusalem, Israel
    J Biol Chem 264:20297-302. 1989
    We have deleted the chromosomal ant gene from Escherichia coli by substitution with the kan gene, which encodes kanamycin resistance. The delta ant strains obtained cannot adapt to high sodium concentrations (700 mM, pH 6...
  67. ncbi Expression of a sodium proton antiporter (NhaA) in Escherichia coli is induced by Na+ and Li+ ions
    R Karpel
    Division of Microbial and Molecular Ecology, Hadassah Medical School, Hebrew University of Jerusalem, Israel
    J Biol Chem 266:21753-9. 1991
    Regulation of expression of nhaA, the gene which encodes a Na+/H+ antiporter in Escherichia coli has been studied...
  68. pmc Characterization and mapping of a major Na+/H+ antiporter gene of Escherichia coli
    P Thelen
    Department of Molecular Biology and Microbiology, Tufts University Medical School, Boston, Massachusetts 02111
    J Bacteriol 173:6553-7. 1991
    ..An isogenic nhaA (formerly antA) deletion strain, however, is not significantly different from wild type in this respect...
  69. ncbi Overproduction and purification of a functional Na+/H+ antiporter coded by nhaA (ant) from Escherichia coli
    D Taglicht
    Institute of Life Sciences, Hebrew University, Jerusalem, Israel
    J Biol Chem 266:11289-94. 1991
    A Na+/H+ antiporter coded by the nhaA (ant) gene of Escherichia coli has been overproduced and purified...
  70. pmc Escherichia coli is able to grow with negligible sodium ion extrusion activity at alkaline pH
    T Ohyama
    Faculty of Pharmaceutical Sciences, Chiba University, Japan
    J Bacteriol 174:7743-9. 1992
    The Escherichia coli mutant NM81, which is deficient in the nhaA gene for the sodium/proton antiporter, still has a sodium ion extrusion activity because of a second antiporter encoded by nhaB (E. Padan, N. Maisler, D. Taglicht, R...
  71. ncbi Histidine 225, a residue of the NhaA-Na+/H+ antiporter of Escherichia coli is exposed and faces the cell exterior
    Y Olami
    Division of Microbial and Molecular Ecology, The Hebrew University of Jerusalem, 91904 Jerusalem, Israel
    J Biol Chem 272:1761-8. 1997
    Cysteine residues were found nonessential in the mechanism of the NhaA antiporter activity of Escherichia coli...
  72. pmc Screening of environmental DNA libraries for the presence of genes conferring Na(+)(Li(+))/H(+) antiporter activity on Escherichia coli: characterization of the recovered genes and the corresponding gene products
    A Majernik
    Institute of Animal Biochemistry and Genetics, Slovak Academy of Sciences, 90028 Ivanka pri Dunaji, Slovak Republic
    J Bacteriol 183:6645-53. 2001
    ..bp) derived from pAM1 contained a gene (1,185 bp) which encodes a novel Na(+)/H(+) antiporter belonging to the NhaA family. The insert of pAM3 harbored the DNA region of E. coli K-12 containing nhaA, nhaR, and gef...
  73. pmc Functional role of polar amino acid residues in Na+/H+ exchangers
    C A Wiebe
    Department of Biochemistry, Faculty of Medicine, University of Alberta, 347 Medical Science Building, Edmonton, Alberta, Canada T6G 2H7
    Biochem J 357:1-10. 2001
    ..Histidine residues have been shown to be important in the function of the E. coli Na(+)/H(+) exchanger NhaA and in the yeast Na(+)/H(+) exchanger sod2. In E...
  74. ncbi NhaR, a protein homologous to a family of bacterial regulatory proteins (LysR), regulates nhaA, the sodium proton antiporter gene in Escherichia coli
    O Rahav-Manor
    Division of Microbial and Molecular Ecology, Hadassah Medical School, Hebrew University of Jerusalem, Israel
    J Biol Chem 267:10433-8. 1992
    ..C. (1988) Proc. Natl. Acad. Sci. U. S. A. 85, 6602-6606). In this work we show that nhaR is a regulator of nhaA, a gene encoding a Na+/H+ antiporter in Escherichia coli...
  75. ncbi Na(+)/H(+) antiporters
    E Padan
    Department of Microbial and Molecular Ecology, Institute of Life Sciences, Hebrew University of Jerusalem, Israel
    Biochim Biophys Acta 1505:144-57. 2001
    ..Structure and function studies of a purified antiporter protein have as yet been conducted mainly with NhaA, the key Na(+)/H(+) antiporter of Escherichia coli...
  76. pmc Transcription of nhaA, the main Na(+)/H(+) antiporter of Escherichia coli, is regulated by Na(+) and growth phase
    N Dover
    Division of Microbial and Molecular Ecology, Institute of Life Sciences, The Hebrew University of Jerusalem, 91904 Jerusalem, Israel
    J Bacteriol 183:644-53. 2001
    The transcription of nhaA, encoding the main Na(+)/H(+) antiporter of Escherichia coli, is induced by Na(+), regulated by NhaR, and affected by H-NS...
  77. ncbi Three-dimensional structure of the ion-coupled transport protein NhaA
    K A Williams
    Department of Structural Biology, Max Planck Institute of Biophysics, Frankfurt Main, Germany
    Nature 403:112-5. 2000
    ..b>NhaA is a Na+/H+ antiporter of relative molecular mass 42,000, which is found in the inner membrane of Escherichia coli, ..
  78. pmc Projection structure of NhaA, a secondary transporter from Escherichia coli, at 4.0 A resolution
    K A Williams
    Max Planck Institute of Biophysics, Department of Structural Biology, Heinrich Hoffmann Strasse 7, D 60528 Frankfurt am Main, Germany
    EMBO J 18:3558-63. 1999
    Electron cryomicroscopy of frozen-hydrated two-dimensional crystals of NhaA, a Na+/H+ antiporter from Escherichia coli predicted to have 12 transmembrane alpha-helices, has facilitated the calculation of a projection map of NhaA at 4...
  79. ncbi The molecular mechanism of regulation of the NhaA Na+/H+ antiporter of Escherichia coli, a key transporter in the adaptation to Na+ and H+
    E Padan
    Hebrew University of Jerusalem, Department of Microbial and Molecular Ecology, Jerusalem, Israel
    Novartis Found Symp 221:183-96; discussion 196-9. 1999
    The NhaA Na+/H+ antiporter is the main system responsible for adaptation to Na+ and alkaline pH (in the presence of Na+) in Escherichia coli and many other enteric bacteria. It is under intricate control...
  80. ncbi pH-dependent growth retardation of Escherichia coli caused by overproduction of Na+/H+ antiporter
    H Inoue
    Dept Biotechnology, Faculty of Engineering Sciences, Okayama University, Japan
    Biol Pharm Bull 21:1128-33. 1998
    The overproduction of Na+/H+ antiporter NhaA in Escherichia coli caused growth retardation. Quantities and the activity of the antiporter were studied for their causative roles in terms of this retardation...
  81. ncbi The Na+ and K+ transport deficiency of an E. coli mutant lacking the NhaA and NhaB proteins is apparent and caused by impaired osmoregulation
    M L Verkhovskaya
    Department of Medical Chemistry, Institute of Biomedical Sciences, University of Helsinki, Finland
    FEBS Lett 439:271-4. 1998
    Cells of the E. coli mutant EP432, which lacks the two Na+/H+ antiporters, NhaA and NhaB, have been reported to have an impaired sodium transport activity (Harel-Bronstein et al. (1995) J. Biol. Chem. 270, 3816-3822)...
  82. ncbi pH dependence of the function of sodium ion extrusion systems in Escherichia coli
    T Sakuma
    Faculty of Pharmaceutical Sciences, Chiba University, 1 33, Yayoi cho, Inage Ku, Chiba 263, Japan
    Biochim Biophys Acta 1363:231-7. 1998
    Escherichia coli has three systems for sodium ion extrusion, NhaA, NhaB and ChaA...
  83. ncbi Probing the conformation of NhaA, a Na+/H+ antiporter from Escherichia coli, with trypsin
    A Rothman
    Alexander Silberman Institute of Life Sciences, Hebrew University of Jerusalem, Israel
    Biochemistry 36:14572-6. 1997
    One of the most striking features of NhaA, an Escherichia coli Na+/H+ antiporter, is its extreme sensitivity to pH. The activity of NhaA increases 2000-fold between pH 6.5 and 8.5...
  84. ncbi Identification and characterization of functional residues in a Na+/H+ antiporter (NhaA) from Escherichia coli by random mutagenesis
    T Noumi
    Department of Biotechnology, Faculty of Engineering Sciences, Okayama University
    J Biochem 121:661-70. 1997
    Forty-one mutants were isolated by means of random PCR mutagenesis of the Escherichia coli Na+/H+ antiporter (nhaA), which could not support the growth of a nhaAnhaB mutant (HIT delta AB-) on plates containing 0.15 M LiCl (pH 7.5) or 0...
  85. ncbi High level production of functional antibody Fab fragments in an oxidizing bacterial cytoplasm
    Miro Venturi
    Max Planck Institut fur Biophysik, Abteilung für Molekulare Membranbiologie, Heinrich Hoffmann Str 7, Frankfurt am Main, D 60528, Germany
    J Mol Biol 315:1-8. 2002
    ..pFAB1 we tested the cytoplasmic expression of two Fab fragments, which recognize the integral membrane protein NhaA, a bacterial Na(+)/H(+) antiporter. These antibodies differ in terms of DNA sequence and stability...
  86. ncbi Production and characterization of monoclonal antibodies directed against native epitopes of NhaA, the Na+/H+ antiporter of Escherichia coli
    E Padan
    Division of Microbial and Molecular Ecology, The Hebrew University of Jerusalem, Israel
    FEBS Lett 441:53-8. 1998
    ..In an attempt to raise monoclonal antibodies against the NhaA Na+/H+ antiporter of Escherichia coli we encountered difficulties in the screening procedure, which is based on the ..
  87. ncbi Physiological role of nhaB, a specific Na+/H+ antiporter in Escherichia coli
    E Pinner
    Division of Microbial and Molecular Ecology, Hebrew University of Jerusalem, Israel
    J Biol Chem 268:1729-34. 1993
    ..267, 11064-11068). In order to elucidate the role of nhaB in Na+ and H+ ions physiology and its interaction with nhaA, we generated mutants in which the chromosomal gene has been inactivated by insertion/deletion...
  88. ncbi A pH-dependent conformational change of NhaA Na(+)/H(+) antiporter of Escherichia coli involves loop VIII-IX, plays a role in the pH response of the protein, and is maintained by the pure protein in dodecyl maltoside
    Y Gerchman
    Division of Microbial and Molecular Ecology, Institute of Life Sciences, Hebrew University of Jerusalem, 91904 Jerusalem, Israel
    J Biol Chem 274:24617-24. 1999
    Digestion with trypsin of purified His-tagged NhaA in a solution of dodecyl maltoside yields two fragments at alkaline pH but only one fragment at acidic pH...
  89. ncbi Controlled unfolding and refolding of a single sodium-proton antiporter using atomic force microscopy
    Alexej Kedrov
    BIOTEC, University of Technology, Dresden, Germany
    J Mol Biol 340:1143-52. 2004
    Single-molecule force-spectroscopy was employed to unfold and refold single sodium-proton antiporters (NhaA) of Escherichia coli from membrane patches...
  90. ncbi Regulatory components, including integration host factor, CysB and H-NS, that influence pH responses in Escherichia coli
    R J Rowbury
    Department of Biology Darwin, University College London, UK
    Lett Appl Microbiol 24:319-28. 1997
    ..DNA gyrase; tolerance is reduced by NaCl but not affected by Fe3+, Fe2+, glucose/cAMP or by lrp, him, fur, hns or nhaA/B lesions. Alkali tolerance (habituation), induced at pH0 8.5-9...
  91. ncbi Molecular model of the Escherichia coli Na1/H1 antiporter NhaA
    A W Ravna
    Department of Pharmacology, Institute of Medical Biology, University of Tromsø, N 9037 Tromsø, Norway
    Receptors Channels 7:319-28. 2001
    ..electron density projection map of the ion-coupled membrane protein Escherichia coli Na+/H+ antiporter (NhaA) was recently published...

Research Grants4

  1. Time Domain EPR Spectrometer
    ALBERT BETH; Fiscal Year: 2005
    ..Equitable utilization of the equipment will be assured by an internal oversight committee and day to- day operation will be handled by an experienced EPR spectroscopist. ..
  2. Vanderbilt Summer Research Experience for Undergraduates
    ALBERT BETH; Fiscal Year: 2006
    ..Participants will be asked to annually update their career paths on a Program web site so that long-term impact of this training experience can be evaluated. ..
  3. Spectroscopic Studies of EGF-Receptor Interactions
    ALBERT BETH; Fiscal Year: 2006
    ..abstract_text> ..
  4. EPR STUDIES OF MEMBRANE PROTEINS
    ALBERT BETH; Fiscal Year: 2007
    ..2? To help accomplish these goals, new molecular probes,[unreadable] spectroscopic methods, and data analysis methods will be developed which[unreadable] will be of utility in the biomedical research community.[unreadable]..