mutM

Summary

Gene Symbol: mutM
Description: formamidopyrimidine/5-formyluracil/ 5-hydroxymethyluracil DNA glycosylase
Alias: ECK3625, JW3610, fpg
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. ncbi Recognition and conversion of single- and double-stranded oligonucleotide substrates by 8-oxoguanine-DNA glycosylase from Escherichia coli
    A A Ishchenko
    Novosibirsk Institute of Bioorganic Chemistry, Russian Academy of Sciences, Russia
    Biochemistry (Mosc) 62:204-11. 1997
  2. ncbi Excision of 5,6-dihydroxy-5,6-dihydrothymine, 5,6-dihydrothymine, and 5-hydroxycytosine from defined sequence oligonucleotides by Escherichia coli endonuclease III and Fpg proteins: kinetic and mechanistic aspects
    C D'Ham
    Laboratoire Lesions des Acides Nucleiques, Service de Chimie Inorganique et Biologique, Departement de Recherche Fondamentale sur la Matiere Condensee, CEA Grenoble, France
    Biochemistry 38:3335-44. 1999
  3. ncbi Radiation-induced DNA base damage detected in individual aerobic and hypoxic cells with endonuclease III and formamidopyrimidine-glycosylase
    J P Banath
    Medical Biophysics Department, British Columbia Cancer Research Centre, Vancouver, Canada
    Radiat Res 151:550-8. 1999
  4. ncbi Characterization of a novel 8-oxoguanine-DNA glycosylase activity in Escherichia coli and identification of the enzyme as endonuclease VIII
    T K Hazra
    Sealy Center for Molecular Science and Department of Human Biological Chemistry and Genetics, University of Texas Medical Branch, Galveston, Texas 77555, USA
    J Biol Chem 275:27762-7. 2000
  5. ncbi Use of crosslinking for revealing the DNA phosphate groups forming specific contacts with the E. coli Fpg protein
    Svetlana Kuznetsova
    Laboratory of Nucleic Acids Chemistry, Department of Chemistry, Moscow State University, Moscow 119899, Russia
    Biochimie 85:511-9. 2003
  6. ncbi In vivo transcription of nrdAB operon and of grxA and fpg genes is triggered in Escherichia coli lacking both thioredoxin and glutaredoxin 1 or thioredoxin and glutathione, respectively
    R Gallardo-Madueno
    Departamento de Bioquimica y Biologia Molecular, Universidad de Cordoba, 14071 Cordoba, Espana
    J Biol Chem 273:18382-8. 1998
  7. ncbi Activity of Escherichia coli DNA-glycosylases on DNA damaged by methylating and ethylating agents and influence of 3-substituted adenine derivatives
    B Tudek
    Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw
    Mutat Res 407:169-76. 1998
  8. doi Increased resistance to oxidative DNA damage of trabecular meshwork cells by E. coli FPG gene transfection
    M Foresta
    Molecular Mutagenesis and DNA Repair Unit, Istituto Nazionale Ricerca Cancro, Genoa, Italy
    Free Radic Res 45:751-8. 2011
  9. ncbi Accelerated repair and reduced mutagenicity of oxidative DNA damage in human bladder cells expressing the E. coli FPG protein
    Monica Ropolo
    Department of Translational Oncology, Experimental Oncology B Laboratory, Istituto Nazionale Ricerca Cancro, Largo Rosanna Benzi n 10, 16132 Genova, Italy
    Int J Cancer 118:1628-34. 2006
  10. ncbi Role of the N-terminal proline residue in the catalytic activities of the Escherichia coli Fpg protein
    O M Sidorkina
    Groupe Reparation des lesions Radio et Chimio Induites, UMR 8532 Centre National de la Recherche Scientifique, Institut Gustave Roussy, 39, rue Camille Desmoulins, 94805 Villejuif Cedex, France
    J Biol Chem 275:9924-9. 2000

Research Grants

Scientific Experts

  • Svetlana Kuznetsova
  • Prasad Reddy
  • G K Kuipers
  • Martine E Lomax
  • Yun Song Lee
  • Susan S Wallace
  • Robert G Fowler
  • B Castaing
  • Michelle L Hamm
  • Dmitry O Zharkov
  • Rou Jia Sung
  • Arthur P Grollman
  • Vladimir V Koval
  • J Laval
  • Georgy A Nevinsky
  • Viktoriya S Sidorenko
  • Alexander A Ishchenko
  • Nikita A Kuznetsov
  • Kun Song
  • Olga S Fedorova
  • O M Sidorkina
  • Kenneth T Douglas
  • Elzbieta Speina
  • Anthony W Francis
  • Rémy Le Meur
  • A A Ishchenko
  • Michael Zhang
  • Gregory L Verdine
  • Yan Qi
  • A C T Silva-Júnior
  • Elena I Zaika
  • Sheila S David
  • M Foresta
  • Rotem Gilboa
  • C M Gifford
  • Conceição A S A Minetti
  • Damian Gawel
  • Murat K Saparbaev
  • Nirmala Krishnamurthy
  • Ella Rotman
  • Carlos de Los Santos
  • Carlos Simmerling
  • Monica Ropolo
  • Maria Rogacheva
  • Michael B Harbut
  • Robert A Rieger
  • P W Chang
  • Garry W Buchko
  • Maria V Rogacheva
  • M V Taraneneko
  • Claudia Alessandra Fortes Aiub
  • Rebecca A Perlow-Poehnelt
  • Jacques Laval
  • Barbara Tudek
  • Anton B Guliaev
  • Jessica L Kosa
  • Suse Broyde
  • R S Lloyd
  • Jennifer Wyrzykowski
  • Eileen Matz
  • Kelly M Kroeger
  • Martin G Marinus
  • Tanja Schwerdtle
  • Maria Taranenko
  • M V Taranenko
  • G A Nevinsky
  • Franck Coste
  • Céline Landon
  • Françoise Culard
  • Stephane Goffinont
  • Virginie Nadan
  • S V Kuznetsov
  • Martine Guerin
  • Karine Loth
  • Erik J Spek
  • Joon Myong Song
  • J Jurado
  • I Felzenszwalb
  • L M B O Asad
  • N R Asad
  • G Frosina
  • A Izzotti
  • M Longobardi
  • C Cartiglia
  • S C Sacca
  • O V Lavrukhin
  • M H David-Cordonnier
  • C V Privezentzev
  • T K Hazra
  • K Asagoshi

Detail Information

Publications94

  1. ncbi Recognition and conversion of single- and double-stranded oligonucleotide substrates by 8-oxoguanine-DNA glycosylase from Escherichia coli
    A A Ishchenko
    Novosibirsk Institute of Bioorganic Chemistry, Russian Academy of Sciences, Russia
    Biochemistry (Mosc) 62:204-11. 1997
    ..These parameters consistently depended on the position of 8-oxoG in single-stranded or double-stranded substrates. A possible mechanism responsible for substrate recognition by 8-oxoguanine-DNA glycosylase is described...
  2. ncbi Excision of 5,6-dihydroxy-5,6-dihydrothymine, 5,6-dihydrothymine, and 5-hydroxycytosine from defined sequence oligonucleotides by Escherichia coli endonuclease III and Fpg proteins: kinetic and mechanistic aspects
    C D'Ham
    Laboratoire Lesions des Acides Nucleiques, Service de Chimie Inorganique et Biologique, Departement de Recherche Fondamentale sur la Matiere Condensee, CEA Grenoble, France
    Biochemistry 38:3335-44. 1999
    ..mechanism of two Escherichia coli repair enzymes: endonuclease III and formamidopyrimidine DNA glycosylase (Fpg). Three techniques of analysis were employed...
  3. ncbi Radiation-induced DNA base damage detected in individual aerobic and hypoxic cells with endonuclease III and formamidopyrimidine-glycosylase
    J P Banath
    Medical Biophysics Department, British Columbia Cancer Research Centre, Vancouver, Canada
    Radiat Res 151:550-8. 1999
    ..0, and (2) the presence of direct DNA strand breaks (>2000-4000 per cell) prevents accurate detection of base damage measured as enzyme-sensitive sites with the alkaline comet method...
  4. ncbi Characterization of a novel 8-oxoguanine-DNA glycosylase activity in Escherichia coli and identification of the enzyme as endonuclease VIII
    T K Hazra
    Sealy Center for Molecular Science and Department of Human Biological Chemistry and Genetics, University of Texas Medical Branch, Galveston, Texas 77555, USA
    J Biol Chem 275:27762-7. 2000
    ..The major G*-DNA glycosylase (OGG), namely, OGG1 in eukaryotes, or MutM in Escherichia coli, excises G* when paired in DNA with C, G, and T, but not A, presumably because removal of G* ..
  5. ncbi Use of crosslinking for revealing the DNA phosphate groups forming specific contacts with the E. coli Fpg protein
    Svetlana Kuznetsova
    Laboratory of Nucleic Acids Chemistry, Department of Chemistry, Moscow State University, Moscow 119899, Russia
    Biochimie 85:511-9. 2003
    ..contacts between DNA phosphate groups and positively charged nucleophilic amino acids from the Escherichia coli Fpg protein play a significant role in DNA-Fpg protein interaction...
  6. ncbi In vivo transcription of nrdAB operon and of grxA and fpg genes is triggered in Escherichia coli lacking both thioredoxin and glutaredoxin 1 or thioredoxin and glutathione, respectively
    R Gallardo-Madueno
    Departamento de Bioquimica y Biologia Molecular, Universidad de Cordoba, 14071 Cordoba, Espana
    J Biol Chem 273:18382-8. 1998
    ..Like grxA expression, the basal level of fpg mRNA, encoding formamidopyrimidine-DNA glycosylase, was increased (about 4-fold) in a trxA gshA double mutant ..
  7. ncbi Activity of Escherichia coli DNA-glycosylases on DNA damaged by methylating and ethylating agents and influence of 3-substituted adenine derivatives
    B Tudek
    Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw
    Mutat Res 407:169-76. 1998
    ..broad substrate specificity including 3- and 7-alkylated purines and the formamidopyrimidine(Fapy)-DNA-glycosylase (Fpg protein) repairing imidazole ring-opened 7-methylguanine...
  8. doi Increased resistance to oxidative DNA damage of trabecular meshwork cells by E. coli FPG gene transfection
    M Foresta
    Molecular Mutagenesis and DNA Repair Unit, Istituto Nazionale Ricerca Cancro, Genoa, Italy
    Free Radic Res 45:751-8. 2011
    ..This situation occurs for Escherichia coli formamidopyrimidine DNA glycosylase (FPG), a major DNA glycosylase that repairs oxidatively damaged DNA...
  9. ncbi Accelerated repair and reduced mutagenicity of oxidative DNA damage in human bladder cells expressing the E. coli FPG protein
    Monica Ropolo
    Department of Translational Oncology, Experimental Oncology B Laboratory, Istituto Nazionale Ricerca Cancro, Largo Rosanna Benzi n 10, 16132 Genova, Italy
    Int J Cancer 118:1628-34. 2006
    ..The formamidopyrimidine DNA glycosylase (FPG) protein from E. coli is endowed with a potent 8-oxoG glycolytic activity coupled with a beta,delta-AP lyase...
  10. ncbi Role of the N-terminal proline residue in the catalytic activities of the Escherichia coli Fpg protein
    O M Sidorkina
    Groupe Reparation des lesions Radio et Chimio Induites, UMR 8532 Centre National de la Recherche Scientifique, Institut Gustave Roussy, 39, rue Camille Desmoulins, 94805 Villejuif Cedex, France
    J Biol Chem 275:9924-9. 2000
    The Escherichia coli Fpg protein is a DNA glycosylase/AP lyase. It removes, in DNA, oxidized purine residues, including the highly mutagenic C8-oxo-guanine (8-oxoG)...
  11. doi The nucleoid-associated protein HU enhances 8-oxoguanine base excision by the formamidopyrimidine-DNA glycosylase
    Rémy Le Meur
    Centre de Biophysique Moleculaire UPR4301, CNRS, rue Charles Sadron, 45 071 Orléans Cedex 02, France Graduate School of Santé, Sciences Biologiques et Chimie du Vivant ED 549, University of Orleans, Orleans, France
    Biochem J 471:13-23. 2015
    ..kinetics and DNA-binding experiments demonstrate that HU enhances the 8-oxoguanine-DNA glycosylase activity of Fpg (formamidopyrimidine-DNA glycosylase) by facilitating the release of the enzyme from its final DNA product (one ..
  12. ncbi The ring fragmentation product of thymidine C5-hydrate when present in DNA is repaired by the Escherichia coli Fpg and Nth proteins
    J Jurado
    Groupe Reparation des lesions Radio et Chimio Induites, UMR 1772 CNRS, Institut Gustave Roussy, Villejuif, France
    Biochemistry 37:7757-63. 1998
    ..substrate for two purified enzymes from Escherichia coli involved in the repair of oxidized bases: the Nth and the Fpg proteins. The Fpg protein removes the alphaRT lesion more efficiently than the Nth protein...
  13. pmc mutM, a second mutator locus in Escherichia coli that generates G.C----T.A transversions
    M Cabrera
    Department of Biology, University of California, Los Angeles 90024
    J Bacteriol 170:5405-7. 1988
    ..C----T.A transversions among base substitutions. The locus, mutM, mapped near the cysE locus, which is at 81 min on the genetic map.
  14. ncbi Proteomic approach to identification of proteins reactive for abasic sites in DNA
    Robert A Rieger
    Department of Pharmacology, Stony Brook University, Stony Brook, NY 11794, USA
    Mol Cell Proteomics 5:858-67. 2006
    ..We report identification of seven proteins from Escherichia coli (AroF, DnaK, MutM, PolA, TnaA, TufA, and UvrA) and two proteins from bakers' yeast (ARC1 and Ygl245wp) reactive for AP sites in this ..
  15. ncbi Inhibition of DNA repair glycosylases by base analogs and tryptophan pyrolysate, Trp-P-1
    Elzbieta Speina
    Department of Molecular Biology, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warszawa, Poland
    Acta Biochim Pol 52:167-78. 2005
    ..coli Fpg protein (formamidopyrimidine -DNA glycosylase)...
  16. ncbi Substrate discrimination by formamidopyrimidine-DNA glycosylase: distinguishing interactions within the active site
    Rebecca A Perlow-Poehnelt
    Department of Biology, New York University, 100 Washington Square East, Room 1009M, New York, New York 10003, USA
    Biochemistry 43:16092-105. 2004
    ..excision repair pathway, which is initiated by DNA glycosylases such as the formamidopyrimidine-DNA glycosylase (Fpg) in Escherichia coli...
  17. ncbi High resolution characterization of formamidopyrimidine-DNA glycosylase interaction with its substrate by chemical cross-linking and mass spectrometry using substrate analogs
    Maria Rogacheva
    Laboratory of Nucleic Acids Chemistry, Department of Chemistry, Moscow State University, Moscow 119992, Russia
    J Biol Chem 281:32353-65. 2006
    Escherichia coli formamidopyrimidine-DNA glycosylase (Fpg) and human 8-oxoguanine-DNA glycosylase (hOgg1) initiate the base excision repair pathway for 7,8-dihydro-8-oxoguanine (8-oxoG) residues present in DNA...
  18. ncbi AlkA protein is the third Escherichia coli DNA repair protein excising a ring fragmentation product of thymine
    C V Privezentzev
    Groupe Reparation des lesions Radio et Chimio Induites, UMR 8532 CNRS, Institut Gustave Roussy, 94805 Villejuif Cedex, France
    Biochemistry 39:14263-8. 2000
    ..It was shown that alphaRT is excised by Escherichia coli Fpg and Nth proteins. Here we report that when present in DNA, alphaRT is, in addition, a substrate for the E...
  19. ncbi Thermodynamic, kinetic, and structural basis for recognition and repair of 8-oxoguanine in DNA by Fpg protein from Escherichia coli
    Alexander A Ishchenko
    Novosibirsk Institute of Bioorganic Chemistry, Siberian Division of the Russian Academy of Sciences, Novosibirsk 630090, Russia
    Biochemistry 41:7540-8. 2002
    ..unit of 8-oxoguanine-containing DNA to its total affinity for Escherichia coli 8-oxoguanine DNA glycosylase (Fpg protein)...
  20. ncbi Probing the requirements for recognition and catalysis in Fpg and MutY with nonpolar adenine isosteres
    Anthony W Francis
    Department of Chemistry, University of Utah, 315 South 1400 East, Salt Lake City, Utah, 84112, USA
    J Am Chem Soc 125:16235-42. 2003
    The Escherichia coli DNA repair enzymes Fpg and MutY are involved in the prevention of mutations resulting from 7,8-dihydro-8-oxo-2'-deoxyguanosine (OG) in DNA...
  21. pmc Role of lysine-57 in the catalytic activities of Escherichia coli formamidopyrimidine-DNA glycosylase (Fpg protein)
    O M Sidorkina
    Groupe Reparation des lesions Radio et Chimio Induites, UMR 1772 CNRS, Institut Gustave Roussy, 94805 Villejuif Cedex, France
    Nucleic Acids Res 26:5351-7. 1998
    The Escherichia coli Fpg protein is involved in the repair of oxidized residues. We examined, by targeted mutagenesis, the effect of the conserved lysine residue at position 57 upon the various catalytic activities of the Fpg protein...
  22. pmc Molecular simulations reveal a common binding mode for glycosylase binding of oxidatively damaged DNA lesions
    Kun Song
    Department of Chemistry, Stony Brook University, Stony Brook, New York 11794 3400, USA
    J Am Chem Soc 129:14536-7. 2007
    ..In Escherichia coli, both lesions are recognized and excised by the DNA glycosylase Fpg. X-ray crystallographic studies have shown that FapydG and 8OG adopt different conformations in the active site of ..
  23. ncbi Properties and biological functions of the NTH and FPG proteins of Escherichia coli: two DNA glycosylases that repair oxidative damage in DNA
    S Boiteux
    LA 147 CNRS, U140 INSERM, Institut Gustave Roussy, Villejuif, France
    J Photochem Photobiol B 19:87-96. 1993
    ..step in this DNA repair pathway is catalysed either by the NTH protein which excises oxidized pyrimidines or by the FPG protein which excises oxidized purines...
  24. pmc Location of the fpg gene on the Escherichia coli chromosome
    S H Coleman
    Department of Biochemistry, Oxford University, UK
    Nucleic Acids Res 19:3999. 1991
  25. pmc Mutator gene of Escherichia coli B
    E C Siegel
    J Bacteriol 94:38-47. 1967
    ..No suppression was found. Recombinants were prepared containing mutS1 and the Treffers mutator gene of E. coli K-12. The effect of the mutator genes appears to be additive...
  26. pmc Sequence-dependent structural variation in DNA undergoing intrahelical inspection by the DNA glycosylase MutM
    Rou Jia Sung
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138, USA
    J Biol Chem 287:18044-54. 2012
    b>MutM, a bacterial DNA-glycosylase, plays a critical role in maintaining genome integrity by catalyzing glycosidic bond cleavage of 8-oxoguanine (oxoG) lesions to initiate base excision DNA repair...
  27. doi The role of Fpg protein in UVC-induced DNA lesions
    A C T Silva-Júnior
    Departamento de Biofisica e Biometria, Instituto de Biologia Roberto Alcantara Gomes, Universidade do Estado do Rio de Janeiro, Rio de Janeiro, RJ, Brazil
    Redox Rep 17:95-100. 2012
    ..The 8-oxoG could be mediated by singlet oxygen ((1)O(2)). The Fpg protein repaired this lesion when it was linked to C (cytosine), whereas the MutY protein repaired 8-oxoG when it ..
  28. pmc Formamidopyrimidine-DNA glycosylase of Escherichia coli: cloning and sequencing of the fpg structural gene and overproduction of the protein
    S Boiteux
    UA 147 CNRS, Institut Gustave Roussy, Villejuif, France
    EMBO J 6:3177-83. 1987
    ..After subcloning a 1.4-kb fragment which contained the Fapy-DNA glycosylase gene (fpg+) was inserted in the plasmids pUC18 and pUC19 yielding pFPG50 and pFPG60 respectively...
  29. ncbi The role of nucleotide excision repair of Escherichia coli in repair of spontaneous and gamma-radiation-induced DNA damage in the lacZalpha gene
    G K Kuipers
    Department of Radiation Oncology, Section Radiobiology, Faculty of Medicine, Vrije Universiteit, Van der Boechorststraat 7, 1081 BT, Amsterdam, The Netherlands
    Mutat Res 460:117-25. 2000
    ..Formamidopyrimidine-DNA glycosylase (Fpg), a repair enzyme which is part of BER, is the most important enzyme to repair 8oxoG...
  30. ncbi New substrates for old enzymes. 5-Hydroxy-2'-deoxycytidine and 5-hydroxy-2'-deoxyuridine are substrates for Escherichia coli endonuclease III and formamidopyrimidine DNA N-glycosylase, while 5-hydroxy-2'-deoxyuridine is a substrate for uracil DNA N-glycos
    Z Hatahet
    Department of Microbiology and Molecular Genetics, University of Vermont, Burlington 05405
    J Biol Chem 269:18814-20. 1994
    ..5-OHdC and 5-OHdU are substrates for Escherichia coli endonuclease III and formamidopyrimidine DNA N-glycosylase (FPG). 5-OHdU is also a substrate for uracil DNA N-glycosylase...
  31. pmc Delta-elimination in the repair of AP (apurinic/apyrimidinic) sites in DNA
    V Bailly
    Department of Biochemistry, Faculty of Sciences, University of Liege, Belgium
    Biochem J 261:707-13. 1989
    ..These repair pathways, involving a delta-elimination step, may be operational not only in E. coli repairing its DNA containing a formamido-pyrimidine lesion, but also in mammalian cells repairing their nuclear DNA containing AP sites...
  32. pmc Structural and biochemical analysis of DNA helix invasion by the bacterial 8-oxoguanine DNA glycosylase MutM
    Rou Jia Sung
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138, USA
    J Biol Chem 288:10012-23. 2013
    b>MutM is a bacterial DNA glycosylase that serves as the first line of defense against the highly mutagenic 8-oxoguanine (oxoG) lesion, catalyzing glycosidic bond cleavage of oxoG to initiate base excision DNA repair...
  33. pmc Mechanism of DNA strand nicking at apurinic/apyrimidinic sites by Escherichia coli [formamidopyrimidine]DNA glycosylase
    V Bailly
    Laboratoire de Biochimie, Faculte des Sciences, Universite de Liege, Belgium
    Biochem J 262:581-9. 1989
    ..The enzyme is without action on a 3'-terminal base-free deoxyribose or on a 3'-terminal base-free unsaturated sugar produced by a beta-elimination reaction nicking the DNA strand 3' to an apurinic or apyrimidinic site...
  34. ncbi Overexpression and rapid purification of Escherichia coli formamidopyrimidine-DNA glycosylase
    Prasad Reddy
    Chemical Science and Technology Laboratory, National Institute of Standards and Technology, Gaithersburg, MD, USA
    Protein Expr Purif 34:126-33. 2004
    Formamidopyrimidine DNA glycosylase (Fpg) is a DNA glycosylase with an associated AP lyase activity. As a DNA repair enzyme, Fpg excises several modified bases from DNA associated with exposure to oxidizing agents such as free radicals...
  35. pmc Pre-steady-state kinetics shows differences in processing of various DNA lesions by Escherichia coli formamidopyrimidine-DNA glycosylase
    Vladimir V Koval
    Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, Novosibirsk 630090, Russia
    Nucleic Acids Res 32:926-35. 2004
    Formamidopyrimidine-DNA-glycosylase (Fpg protein, MutM) catalyses excision of 8-oxoguanine (8-oxoG) and other oxidatively damaged purines from DNA in a glycosylase/apurinic/apyrimidinic-lyase reaction...
  36. ncbi Chloroethylnitrosourea-derived ethano cytosine and adenine adducts are substrates for Escherichia coli glycosylases excising analogous etheno adducts
    Anton B Guliaev
    Life Sciences Division, Lawrence Berkeley National Laboratory, University of California, Berkeley, CA 94720, USA
    DNA Repair (Amst) 3:1311-21. 2004
    ..However, also as shown by MD, the stacking interaction between the EC base and Phe 30 in the Mug active site is reduced as compared to the epsilonC base, which could account for the lower EC activity observed in this study...
  37. ncbi RecN and RecG are required for Escherichia coli survival of Bleomycin-induced damage
    Jessica L Kosa
    Biological Engineering Division, Department of Chemistry, Massachusetts Institute of Technology, Rm 56 689, 77 Massachusetts Avenue, Cambridge 02139, USA
    Mutat Res 554:149-57. 2004
    ..Escherichia coli recA cells were far more sensitive than were uvrA, dam-3, and mutM mutY strains, underscoring the importance of RecA to survival...
  38. ncbi Arsenite and its biomethylated metabolites interfere with the formation and repair of stable BPDE-induced DNA adducts in human cells and impair XPAzf and Fpg
    Tanja Schwerdtle
    Institute of Food Chemistry and Toxicology, University of Karlsruhe, Postfach 6980, D 76128, Karlsruhe, Germany
    DNA Repair (Amst) 2:1449-63. 2003
    ..with the zinc finger domain of the human XPA protein (XPAzf) and the Escherichia coli zinc finger protein Fpg, involved in NER and BER, respectively, have been investigated...
  39. ncbi Chemical rearrangement and repair pathways of 1,N6-ethenoadenine
    Elzbieta Speina
    Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawinskiego 5A, 02 106 Warsaw, Poland
    Mutat Res 531:205-17. 2003
    ..paired with thymine and cytosine is efficiently excised by Escherichia coli formamidopirymidine DNA N-glycosylase (Fpg), and thymine glycol DNA N-glycosylases from E. coli (Nth) and Saccharomyces cerevisiae (Ntg2)...
  40. ncbi Participation of BER and NER pathways in the repair of DNA lesions induced at low N-nitrosodiethylamine concentrations
    Claudia Alessandra Fortes Aiub
    Departamento de Biofisica e Biometria, Instituto de Biologia Roberto Alcantara Gomes, Universidade do Estado do Rio de Janeiro, Av 28 de Setembro, 87 fds, 4 andar, 20551 030 Rio de Janeiro, Brazil
    Toxicol Lett 154:133-42. 2004
    ..through cell survival, in different single and double Escherichia coli DNA repair mutants (uvrA, uvrB, uvrC, fpg, nth, xthA, fpg/nth, uvrA/fpg, fpg/xthA, mutY, and fpg/mutY), using pre-incubation periods of 90 min...
  41. ncbi [New non-hydrolyzable substrate analogs for 8-oxoguanine-DNA glycosylases]
    M V Taraneneko
    Mol Biol (Mosk) 38:858-68. 2004
    ..The Escherichia coli formamidopyrimidine-DNA glycosylase (Fpg) and human 8-oxoguanine-DNA glycosylase (hOGG1) are DNA base excision repair enzymes that catalyze the removal of 7,..
  42. ncbi Cross-linking of Escherichia coli formamidopyrymidine-DNA glycosylase to DNA duplexes containing photoactivatable phenyl(trifluoromethyl)diazirine groups
    M V Taranenko
    Chemistry Department, Moscow State University, Moscow, Russia
    Nucleosides Nucleotides Nucleic Acids 22:1505-7. 2003
    New reactive analogs of substrates for DNA repair enzyme E. coli Fpg protein containing the residues of 8-oxoguanine and photoactivatable phenyl(trifluoromethyl)diazirine groups were synthesized...
  43. ncbi 8-OxoA inhibits the incision of an AP site by the DNA glycosylases Fpg, Nth and the AP endonuclease HAP1
    Martine E Lomax
    Medical Research Council, Radiation and Genome Stability Unit, Harwell, Didcot, Oxfordshire, OX11 0RD, United Kingdom
    Radiat Res 163:79-84. 2005
    ..The influence 8-oxoA has on the incision of the AP site by the E. coli glycosylases Fpg and Nth protein and the human AP endonuclease HAP1 was assessed...
  44. pmc Role of accessory DNA polymerases in DNA replication in Escherichia coli: analysis of the dnaX36 mutator mutant
    Damian Gawel
    Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709, USA
    J Bacteriol 190:1730-42. 2008
    ..The present results, including lack of interaction of dnaX36 with mutM, mutY, and recA defects, support our assumption that dnaX36-mediated mutations originate as errors of replication ..
  45. ncbi Enzymatic properties of Escherichia coli and human 7,8-dihydro-8-oxoguanine DNA glycosylases
    K Asagoshi
    Department of Mathematical and Life Sciences, Graduate School of Science, Hiroshima University, Higashi Hiroshima 739 8526, Japan
    Nucleic Acids Symp Ser . 2000
    ..The repair activity of Fpg and hOGG1 proteins were compared using oligonucleotide substrates containing me-Fapy and 8-oxoG.
  46. ncbi Solution-state NMR investigation of DNA binding interactions in Escherichia coli formamidopyrimidine-DNA glycosylase (Fpg): a dynamic description of the DNA/protein interface
    Garry W Buchko
    Biological Sciences Division, Battelle, Pacific Northwest National Laboratory, P O Box 999, Richland, WA 99352, USA
    DNA Repair (Amst) 4:327-39. 2005
    Formamidopyrimidine-DNA glycosylase (Fpg) is a base excision repair (BER) protein that removes oxidative DNA lesions...
  47. doi Stable isotope-labeling of DNA repair proteins, and their purification and characterization
    Prasad T Reddy
    Biochemical Science Division, National Institute of Standards and Technology, Gaithersburg, MD, USA
    Protein Expr Purif 78:94-101. 2011
    ..e., (15)N-labeled Escherichia coli formamidopyrimidine DNA glycosylase (Fpg) and (15)N-labeled human 8-oxoguanine-DNA glycosylase (hOGG1)...
  48. doi Real-time studies of conformational dynamics of the repair enzyme E. coli formamidopyrimidine-DNA glycosylase and its DNA complexes during catalytic cycle
    Vladimir V Koval
    Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, Lavrentyev Ave 8, Novosibirsk 630090, Russia
    Mutat Res 685:3-10. 2010
    b>Fpg protein from Escherichia coli belongs to the class of DNA glycosylases/abasic site lyases excising several oxidatively damaged purines in the base excision repair pathway...
  49. ncbi Two sequential phosphates 3' adjacent to the 8-oxoguanosine are crucial for lesion excision by E. coli Fpg protein and human 8-oxoguanine-DNA glycosylase
    Maria V Rogacheva
    Laboratory of Nucleic Acids Chemistry, Department of Chemistry, Moscow State University, Moscow 119899, Russia
    Biochimie 87:1079-88. 2005
    Escherichia coli formamidopyrimidine-DNA glycosylase (Fpg) and human 8-oxoguanine-DNA glycosylase (hOGG1) are base excision repair enzymes involved in the 8-oxoguanine (oxoG) repair pathway...
  50. ncbi Increased sensitivity to sparsely ionizing radiation due to excessive base excision in clustered DNA damage sites in Escherichia coli
    P W Chang
    Laboratory of Radiation Biology, Graduate School of Science, Kyoto University, Japan
    Int J Radiat Biol 81:115-23. 2005
    ..In order to clarify the cellular processing and repair mechanisms for radiation-induced clustered DNA damage, we examined the correlation between the levels of DNA glycosylases and the sensitivity to ionizing radiation in Escherichia coli...
  51. doi Correlated cleavage of damaged DNA by bacterial and human 8-oxoguanine-DNA glycosylases
    Viktoriya S Sidorenko
    SB RAS Institute of Chemical Biology and Fundamental Medicine, Novosibirsk 630090, Russia
    Biochemistry 47:8970-6. 2008
    ..We have used a recently developed correlated cleavage assay to investigate whether this mechanism operates for Fpg and OGG1, two structurally unrelated DNA glycosylases that excise an important oxidative lesion, 7,8-dihydro-8-..
  52. pmc Modulation of the turnover of formamidopyrimidine DNA glycosylase
    Michael B Harbut
    Center for Research on Occupational and Environmental Toxicology, Oregon Health and Science University, 3181 SW Sam Jackson Park Road, L606, Portland, Oregon 97239 3098, USA
    Biochemistry 45:7341-6. 2006
    ..conducted comparing the active site regions of T4-Pdg and the Escherichia coli formamidopyrimidine DNA glycosylase (Fpg). These analyses revealed that His71 in Fpg might perform a similar function to His16 in T4-Pdg...
  53. doi Ionic strength and magnesium affect the specificity of Escherichia coli and human 8-oxoguanine-DNA glycosylases
    Viktoriya S Sidorenko
    SB RAS Institute of Chemical Biology and Fundamental Medicine, Novosibirsk, Russia
    FEBS J 275:3747-60. 2008
    ..A key element of this system is 8-oxoguanine-DNA glycosylase (Fpg in bacteria, OGG1 in eukaryotes), which must excise 8-oxoG from 8-oxoG:C pairs but not from 8-oxoG:A...
  54. ncbi Computational analysis of the mode of binding of 8-oxoguanine to formamidopyrimidine-DNA glycosylase
    Kun Song
    Department of Chemistry, Stony Brook University, Stony Brook, New York 11794 3400, USA
    Biochemistry 45:10886-94. 2006
    ..In bacteria, 8OG is excised by formamidopyrimidine glycosylase (Fpg) as the initial step in base excision repair...
  55. pmc MutM, a protein that prevents G.C----T.A transversions, is formamidopyrimidine-DNA glycosylase
    M L Michaels
    Molecular Biology Institute, University of California, Los Angeles 90024
    Nucleic Acids Res 19:3629-32. 1991
    We have cloned chromosomal DNA bordering an insert that inactivates mutM. Sequencing of this clone has revealed that the insertion element is located between the promoter and structural gene for formamidopyrimidine-DNA glycosylase (Fapy-..
  56. ncbi Pre-steady-state kinetic study of substrate specificity of Escherichia coli formamidopyrimidine--DNA glycosylase
    Nikita A Kuznetsov
    Institute of Chemical Biology and Fundamental Medicine, Novosibirsk State University, Novosibirsk 630090, Russia
    Biochemistry 46:424-35. 2007
    Formamidopyrimidine-DNA glycosylase (Fpg) is responsible for removal of 8-oxoguanine (8-oxoG) and other oxidized purine lesions from DNA and can also excise some oxidatively modified pyrimidines [such as dihydrouracil (DHU)]...
  57. pmc A continuous hyperchromicity assay to characterize the kinetics and thermodynamics of DNA lesion recognition and base excision
    Conceição A S A Minetti
    Department of Chemistry and Chemical Biology, Rutgers, The State University of New Jersey, 610 Taylor Road, Piscataway, NJ 08854, USA
    Proc Natl Acad Sci U S A 105:70-5. 2008
    ..We apply the assay to the bifunctional formamidopyrimidine glycosylase (Fpg) repair enzyme (E) that recognizes an 8-oxodG lesion within a 13-mer duplex substrate (S)...
  58. pmc The mutT defect does not elevate chromosomal fragmentation in Escherichia coli because of the surprisingly low levels of MutM/MutY-recognized DNA modifications
    Ella Rotman
    Department of Microbiology, University of Illinois at Urbana Champaign, IL 61801 3709, USA
    J Bacteriol 189:6976-88. 2007
    ..Overproduction of the MutM and MutY DNA glycosylases, both acting on DNA containing 8-oxo-G, still yields no lethality in mutT recBC double ..
  59. pmc Unusual structural features of hydantoin lesions translate into efficient recognition by Escherichia coli Fpg
    Nirmala Krishnamurthy
    Department of Chemistry, University of Utah, 315 South, 1400 East, Salt Lake City, Utah 84112, USA
    Biochemistry 46:9355-65. 2007
    ..shown that duplexes containing Gh and Sp are substrates for the base excision repair glycosylase Escherichia coli Fpg (EcFpg)...
  60. pmc Selection of a mutant of Escherichia coli which has high mutation rates
    R B Helling
    J Bacteriol 96:975-80. 1968
    ..This mutation (mut-2) and the Treffers' mutation (mutT1) mapped at approximately the same position to the right of leu. Specificity studies showed that the two mutations differ in rates of mutation produced...
  61. ncbi Photochemical cross-linking of Escherichia coli Fpg protein to DNA duplexes containing phenyl(trifluoromethyl)diazirine groups
    Maria Taranenko
    Laboratory of Nucleic Acids Chemistry, Department of Chemistry, Moscow State University, Moscow, Russia
    Eur J Biochem 270:2945-9. 2003
    Formamidopyrimidine-DNA glycosylase (Fpg protein) of Escherichia coli is a DNA repair enzyme that excises oxidized purine bases, most notably the mutagenic 7-hydro-8-oxoguanine, from damaged DNA...
  62. pmc AP site structural determinants for Fpg specific recognition
    B Castaing
    Centre de Biophysique Moleculaire, UPR 4301 affiliated to the University of Orleans, CNRS, rue Charles Sadron, 45071 Orleans Cedex 2, France
    Nucleic Acids Res 27:608-15. 1999
    The binding of Escherichia coli and Lactococcus lactis Fapy-DNA glyosylase (Fpg) proteins to DNA containing either cyclic or non-cyclic abasic (AP) site analogs was investigated by electrophoretic mobility shift assay (EMSA) and by ..
  63. ncbi Effect of single mutations on the structural dynamics of a DNA repair enzyme, the Escherichia coli formamidopyrimidine-DNA glycosylase--a fluorescence study using tryptophan residues as reporter groups
    S V Kuznetsov
    Museum national d histoire naturelle, Laboratoire de Photobiologie INSERM U 312, Paris, France
    Eur J Biochem 253:413-20. 1998
    The effects on the structure dynamics of the Escherichia coli wild-type formamidopyrimidine-DNA glycosylase (Fpg) protein of the single mutations Lys57-->Gly (FpgK57G), Pro2-->Gly (FpgP2G) and Pro2-->Glu (FpgP2E) were studied by ..
  64. ncbi Near ultraviolet radiation (UVA and UVB) causes a formamidopyrimidine glycosylase-dependent increase in G to T transversions
    C M Palmer
    Department of Biology, McMaster University, Hamilton, Ontario, Canada
    Photochem Photobiol 65:543-9. 1997
    ..We have recently shown that Escherichia coli strains deficient in Fapy glycosylase (fpg) are hypersensitive to the lethal effects of UVA but not far-UV (UVC), suggesting lesions recognized by this enzyme ..
  65. ncbi DNA base damage induced by ionizing radiation recognized by Escherichia coli UvrABC nuclease but not Nth or Fpg proteins
    T Roldan-Arjona
    Imperial Cancer Research Fund, Clare Hall Laboratories, South Mimms Hertfordshire, United Kingdom
    Mol Carcinog 16:188-96. 1996
    ..DNA glycosylases of Escherichia coli, endonuclease III (Nth) and formamidopyrimidine-DNA glycosylase (Fpg), which have associated AP lyase activities...
  66. pmc Cleavage and binding of a DNA fragment containing a single 8-oxoguanine by wild type and mutant FPG proteins
    B Castaing
    Laboratoire de Biologie Structurale du CNRS, Gif sur Yvette, France
    Nucleic Acids Res 21:2899-905. 1993
    ..single 7,8-dihydro-8-oxoguanine (8-OxoG) residue was used to study the enzymatic and DNA binding properties of the Fpg protein from E. coli...
  67. ncbi Ultraviolet-B-induced damage to Escherichia coli Fpg protein
    O M Sidorkina
    Groupe Reparation des lesions Radio et Chimio Induites, Institut Gustave Roussy, Villejuif, France
    Photochem Photobiol 69:658-63. 1999
    ..of UVB light (290 < or = lambda < or = 320 nm) on the structure and enzymatic activities of Escherichia coli Fpg protein (2,6-diamino-4-hydroxy-5N-methylformamidopyrimidine-DNA glycosylase), a DNA repair enzyme containing a zinc ..
  68. pmc Physical association of the 2,6-diamino-4-hydroxy-5N-formamidopyrimidine-DNA glycosylase of Escherichia coli and an activity nicking DNA at apurinic/apyrimidinic sites
    T R O'Connor
    Unité de Recherche Associee 158, Centre National de la Recherche Scientifique, Institut Gustave Roussy, Villejuif, France
    Proc Natl Acad Sci U S A 86:5222-6. 1989
    ..2,6-diamino-4-hydroxy-5N-formamidopyrimidine (Fapy)-DNA glycosylase of Escherichia coli, which is coded for by the fpg gene, excises purine bases with ring-opened imidazoles...
  69. ncbi Isolation of a formamidopyrimidine-DNA glycosylase (fpg) mutant of Escherichia coli K12
    S Boiteux
    UA 147 CNRS, U 140 INSERM, Institut Gustave Roussy, Villejuif, France
    Mol Gen Genet 215:300-5. 1989
    The fpg+ gene of Escherichia coli coding for formamidopyrimidine-DNA glycosylase was previously cloned on a multicopy plasmid...
  70. pmc 8-oxoguanine (8-hydroxyguanine) DNA glycosylase and its substrate specificity
    J Tchou
    Biology Division, National Cancer Center Research Institute, Tokyo, Japan
    Proc Natl Acad Sci U S A 88:4690-4. 1991
    Substrate specificities of FPG protein (also known as formamidopyrimidine DNA glycosylase) and 8-hydroxyguanine endonuclease were compared by using defined duplex oligodeoxynucleotides containing single residues of 8-oxo-7,8-dihydro-2'-..
  71. pmc DNA containing a chemically reduced apurinic site is a high affinity ligand for the E. coli formamidopyrimidine-DNA glycosylase
    B Castaing
    Centre de Genetique Moleculaire, Universite Pierre et Marie Curie, Gif Yvette, France
    Nucleic Acids Res 20:389-94. 1992
    The E. coli Formamidopyrimidine-DNA Glycosylase (FPG protein), a monomeric DNA repair enzyme of 30.2 kDa, was purified to homogeneity in large quantities...
  72. ncbi Substrate specificity of the Escherichia coli Fpg protein (formamidopyrimidine-DNA glycosylase): excision of purine lesions in DNA produced by ionizing radiation or photosensitization
    S Boiteux
    URA 158 CNRS, U 140 INSERM, Institut Gustave Roussy, Villejuif, France
    Biochemistry 31:106-10. 1992
    We have investigated the excision of a variety of modified bases from DNA by the Escherichia coli Fpg protein (formamidopyrimidine-DNA glycosylase) [Boiteux, S., O'Connor, T. R., Lederer, F., Gouyette, A., & Laval, J. (1990) J. Biol...
  73. pmc Escherichia coli Fpg protein and UvrABC endonuclease repair DNA damage induced by methylene blue plus visible light in vivo and in vitro
    H Czeczot
    Groupe Réparation des lésions radio chimioinduites, UA158 CNRS, U140 INSERM, Institut Gustave Roussy, Villejuif, France
    J Bacteriol 173:3419-24. 1991
    ..for transformation efficiency in Escherichia coli mutants defective in either formamidopyrimidine-DNA glycosylase (Fpg protein) and/or UvrABC endonuclease...
  74. ncbi Homogeneous Escherichia coli FPG protein. A DNA glycosylase which excises imidazole ring-opened purines and nicks DNA at apurinic/apyrimidinic sites
    S Boiteux
    UA147 Centre National de la Recherche Scientifique, Institut Gustave Roussy, Villejuif, France
    J Biol Chem 265:3916-22. 1990
    ..Fapy) residues in DNA is performed by a Fapy-DNA glycosylase activity which is encoded for by the fpg gene in Escherichia coli...
  75. ncbi Excision of 5'-terminal deoxyribose phosphate from damaged DNA is catalyzed by the Fpg protein of Escherichia coli
    R J Graves
    Groupe Reparation des lesions Radio et Chimio Induites, Centre National de la Recherche Scientifique URA147, Institut Gustave Roussy, Villejuif, France
    J Biol Chem 267:14429-35. 1992
    Homogeneous Fpg protein of Escherichia coli has DNA glycosylase activity which excises some purine bases with damaged imidazole rings, and an activity excising deoxyribose (dR) from DNA at abasic (AP) sites leaving a gap bordered by 5'- ..
  76. pmc Nitric oxide-induced homologous recombination in Escherichia coli is promoted by DNA glycosylases
    Erik J Spek
    Division of Biological Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    J Bacteriol 184:3501-7. 2002
    ..cells can be protected from NO* toxicity by inactivation of the uracil (Ung) or formamidopyrimidine (Fpg) DNA glycosylases but not by inactivation of a 3-methyladenine (AlkA) DNA glycosylase...
  77. ncbi The enigma of endonuclease VIII
    Susan S Wallace
    Department of Microbiology and Molecular Genetics, Markey Center for Molecular Genetics, University of Vermont, 95 Carrigan Drive, Stafford Hall, Burlington, VT 05405 0068, USA
    DNA Repair (Amst) 2:441-53. 2003
  78. ncbi Cross-linking of 2-deoxyribonolactone and its beta-elimination product by base excision repair enzymes
    Kelly M Kroeger
    Department of Chemistry, Johns Hopkins University, 3400 North Charles Street, Baltimore, Maryland 21218, USA
    Biochemistry 42:2449-55. 2003
    ..Two of the enzymes, FPG and NEIL1 known to cleave normal abasic sites (1) by effecting beta,delta-elimination form cross-links to the ..
  79. pmc The Escherichia coli methyl-directed mismatch repair system repairs base pairs containing oxidative lesions
    Jennifer Wyrzykowski
    Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA
    J Bacteriol 185:1701-4. 2003
    ..from MMR recognition of base pairs containing 8-oxoguanine (8-oxoG) based on the finding that overexpression of the MutM oxidative repair protein, which repairs 8-oxoG, can suppress the mutH-dependent increase in transversion mutations...
  80. ncbi Escherichia coli MutY and Fpg utilize a processive mechanism for target location
    Anthony W Francis
    Department of Chemistry, University of Utah, 315 S 1400 E, Salt Lake City, Utah 84112, USA
    Biochemistry 42:801-10. 2003
    MutY and formamidopyrimidine-DNA-glycosylase (Fpg) are base-excision repair (BER) enzymes involved in the 8-oxoguanine repair pathway in Escherichia coli...
  81. ncbi Bistranded oxidized purine damage clusters: induced in DNA by long-wavelength ultraviolet (290-400 nm) radiation?
    Joon Myong Song
    Biology Department, Brookhaven National Laboratory, Upton, New York 11973 5000, USA
    Biochemistry 41:8683-8. 2002
    ..the induction of bistranded clustered oxidized purine and abasic clusters, as recognized by Escherichia coli Fpg protein and E. coli Nfo protein (endonuclease IV), respectively, as well as double-strand breaks...
  82. pmc The genes encoding formamidopyrimidine and MutY DNA glycosylases in Escherichia coli are transcribed as part of complex operons
    C M Gifford
    Department of Microbiology and Molecular Genetics, The Markey Center for Molecular Genetics, The University of Vermont, Burlington, Vermont 05405 0068, USA
    J Bacteriol 181:4223-36. 1999
    Escherichia coli formamidopyrimidine (Fpg) DNA glycosylase and MutY DNA glycosylase are base excision repair proteins that work together to protect cells from the mutagenic effects of the commonly oxidized guanine product 7,8-dihydro-8-..
  83. pmc Multiprobe RNase protection assay analysis of mRNA levels for the Escherichia coli oxidative DNA glycosylase genes under conditions of oxidative stress
    C M Gifford
    Department of Microbiology and Molecular Genetics, The Markey Center for Molecular Genetics, The University of Vermont, Burlington, Vermont 05405 0068, USA
    J Bacteriol 182:5416-24. 2000
    Escherichia coli formamidopyrimidine DNA glycosylase (Fpg), MutY DNA glycosylase, endonuclease VIII, and endonuclease III are oxidative base excision repair DNA glycosylases that remove oxidized bases from DNA, or an incorrect base ..
  84. ncbi Clustered DNA damage, influence on damage excision by XRS5 nuclear extracts and Escherichia coli Nth and Fpg proteins
    M H David-Cordonnier
    Medical Research Council, Radiation and Genome Stability Unit, Harwell, Didcot, Oxon, OX11 0RD, United Kingdom
    J Biol Chem 275:11865-73. 2000
    ..Escherichia coli Nth and Fpg and nuclear extracts from XRS5, a Chinese hamster ovary Ku-deficient cell line, have been used to study the ..
  85. ncbi Single-stranded oligodeoxyribonucleotides are substrates of Fpg protein from Escherichia coli
    A A Ishchenko
    Novosibirsk Institute of Bioorganic Chemistry, Siberian Division of the Russian Academy of Sciences, Russian Federation
    IUBMB Life 48:613-8. 1999
    The interaction of Escherichia coli Fpg protein, which catalyzes excision of several damaged purine bases including 8-oxoguanine (oxoG) from DNA with a set of single- (ss) and double-stranded (ds) 23-mer oligodeoxyribonucleotides (ODNs) ..
  86. ncbi Structural requirements of double and single stranded DNA substrates and inhibitors, including a photoaffinity label, of Fpg protein from Escherichia coli
    A A Ishchenko
    Novosibirsk Institute of Bioorganic Chemistry, Siberian Division of the Russian Academy of Sciences, Novosibirsk
    J Biomol Struct Dyn 17:301-10. 1999
    b>Fpg protein (formamidopyrimidine or 8-oxoguanine DNA glycosylase) from E. coli catalyzes excision of several damaged purine bases, including 8-oxoguanine and 2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine from DNA...
  87. ncbi Involvement of phylogenetically conserved acidic amino acid residues in catalysis by an oxidative DNA damage enzyme formamidopyrimidine glycosylase
    O V Lavrukhin
    Sealy Center for Molecular Science, Department of Human Biological Chemistry and Genetics, University of Texas Medical Branch, Galveston, Texas 77555 1071, USA
    Biochemistry 39:15266-71. 2000
    Formamidopyrimidine glycosylase (Fpg) is an important bacterial base excision repair enzyme, which initiates removal of damaged purines such as the highly mutagenic 8-oxoguanine...
  88. ncbi Structure of formamidopyrimidine-DNA glycosylase covalently complexed to DNA
    Rotem Gilboa
    Department of Inorganic Chemistry and the Laboratory for Structural Chemistry and Biology, The Hebrew University of Jerusalem, Jerusalem 91904, Israel
    J Biol Chem 277:19811-6. 2002
    Formamidopyrimidine-DNA glycosylase (Fpg) is a DNA repair enzyme that excises oxidized purines from damaged DNA...
  89. ncbi Substrate specificity of Fpg (MutM) and hOGG1, two repair glycosylases
    Michelle L Hamm
    Department of Chemistry, University of Richmond, Gottwald B 100, Richmond, Virginia 23173, USA
    J Am Chem Soc 129:7724-5. 2007
  90. ncbi A gene coding for 3-deoxy-D-manno-octulosonic-acid transferase in Escherichia coli. Identification, mapping, cloning, and sequencing
    T Clementz
    Department of Biochemistry, Merck Sharp and Dohme Research Laboratories, Rahway, New Jersey 07065
    J Biol Chem 266:9687-96. 1991
    ..The results demonstrate that overexpression of this single gene product greatly stimulates the incorporation of two stereochemically distinct KDO residues during lipopolysaccharide biosynthesis in extracts of E. coli...
  91. ncbi Substrate discrimination by formamidopyrimidine-DNA glycosylase: a mutational analysis
    Elena I Zaika
    Laboratory of Chemical Biology, Department of Pharmacological Sciences, State University of New York at Stony Brook, Stony Brook, New York 11794, USA
    J Biol Chem 279:4849-61. 2004
    Formamidopyrimidine-DNA glycosylase (Fpg) is a primary participant in the repair of 8-oxoguanine, an abundant oxidative DNA lesion...
  92. ncbi Spectroscopic studies of zinc(II)- and cobalt(II)-associated Escherichia coli formamidopyrimidine-DNA glycosylase: extended X-ray absorption fine structure evidence for a metal-binding domain
    G W Buchko
    Department of Microbiology and Molecular Genetics, University of Vermont, Burlington, Vermont 05405, USA
    Biochemistry 39:12441-9. 2000
    Formamidopyrimidine-DNA glycosylase (Fpg) is a 30.2 kDa protein that plays an important role in the base excision repair of oxidatively damaged DNA in Escherichia coli...
  93. ncbi Interactions among the Escherichia coli mutT, mutM, and mutY damage prevention pathways
    Robert G Fowler
    Department of Biological Sciences, San Jose State University, San Jose, CA 95192, USA
    DNA Repair (Amst) 2:159-73. 2003
    We have investigated in detail the interactions between the Escherichia coli mutT, mutM, and mutY error-prevention systems...
  94. ncbi Recognition of damaged DNA by Escherichia coli Fpg protein: insights from structural and kinetic data
    Dmitry O Zharkov
    Novosibirsk Institute of Chemical Biology and Fundamental Medicine, Siberian Division of Russian Academy of Sciences SB RAS, Lavrentieva Avenue 8, Novosibirsk 630090, Russia
    Mutat Res 531:141-56. 2003
    Formamidopyrimidine-DNA glycosylase (Fpg) excises oxidized purines from damaged DNA...

Research Grants1

  1. Pathway of Spontaneous Mutagenesis in Escherichia coli
    Robert Fowler; Fiscal Year: 2004
    ..Humans possess a number of these specialized DNA polymerases whose activities may be responsible for a significant portion of the human mutational load. ..