mutH

Summary

Gene Symbol: mutH
Description: methyl-directed mismatch repair protein
Alias: ECK2827, JW2799, mutR, prv
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. ncbi Initiation of methyl-directed mismatch repair
    K G Au
    Department of Biochemistry, Duke University Medical Center, Durham, North Carolina 27710
    J Biol Chem 267:12142-8. 1992
  2. ncbi In vitro and in vivo studies of MutS, MutL and MutH mutants: correlation of mismatch repair and DNA recombination
    Murray S Junop
    Laboratory of Molecular Biology, NIDDK, National Institutes of Health, 9000 Rockville Pike, Bldg 5, Room B1 03, Bethesda, MD 20892, USA
    DNA Repair (Amst) 2:387-405. 2003
  3. pmc Site-specific protein modification to identify the MutL interface of MutH
    Grischa H Toedt
    Institut für Biochemie FB 08, Justus Liebig Universitat, Heinrich Buff Ring 58, D 35392 Giessen, Germany
    Nucleic Acids Res 31:819-25. 2003
  4. ncbi Cooperation and competition in mismatch repair: very short-patch repair and methyl-directed mismatch repair in Escherichia coli
    Ashok S Bhagwat
    Department of Chemistry, Wayne State University, Detroit, MI 48202, USA
    Mol Microbiol 44:1421-8. 2002
  5. ncbi Characterization of functional interactions among the Escherichia coli mismatch repair proteins using a bacterial two-hybrid assay
    C A Mansour
    Biology Department, Concordia University, Montreal, Que, Canada
    Mutat Res 485:331-8. 2001
  6. ncbi DNA mismatch repair
    Thomas A Kunkel
    Laboratory of Molecular Genetics and Laboratory of Structural Biology, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709, USA
    Annu Rev Biochem 74:681-710. 2005
  7. ncbi Mutational analysis of the MutH protein from Escherichia coli
    T Loh
    Department of Pharmacology and Molecular Toxicology, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA
    J Biol Chem 276:12113-9. 2001
  8. ncbi MutL-catalyzed ATP hydrolysis is required at a post-UvrD loading step in methyl-directed mismatch repair
    Adam B Robertson
    Department of Biology, University of North Carolina at Chapel Hill, NC 27599, USA
    J Biol Chem 281:19949-59. 2006
  9. pmc Negative regulation of mutS and mutH repair gene expression by the Hfq and RpoS global regulators of Escherichia coli K-12
    H C Tsui
    Department of Microbiology and Molecular Genetics, University of Texas Houston Medical School, 77030 1501, USA
    J Bacteriol 179:7476-87. 1997
  10. ncbi Single-step purifications of His6-MutH, His6-MutL and His6-MutS repair proteins of escherichia coli K-12
    G Feng
    University of Texas, Houston Medical School, USA
    Biotechniques 19:956-65. 1995

Research Grants

  1. A Study of Mutation Dynamics in Biological Systems
    Emmanuel Tannenbaum; Fiscal Year: 2005
  2. 7th Annual Midwest DNA Repair Symposium
    ASHOK BHAGWAT; Fiscal Year: 2005
  3. Improving Phenylbutyrate-based Anticancer Therapy
    ASHOK BHAGWAT; Fiscal Year: 2005
  4. Discovering New Human DNA Repair Genes by Bioinformatics
    ASHOK BHAGWAT; Fiscal Year: 2007

Scientific Experts

  • T A Kunkel
  • Juan Carlos Galan
  • ASHOK BHAGWAT
  • Mark J Schofield
  • Samir Acharya
  • Richard Kolodner
  • EMMANUEL D TANNENBAUM
  • Peter Friedhoff
  • Anna Pluciennik
  • Luis Giron-Monzon
  • Laura Manelyte
  • Jeffrey H Miller
  • Nina Costantino
  • Roger J Heinze
  • Claire G Cupples
  • Rebecka L Bourn
  • Yaroslava Y Polosina
  • Daniela K Jacquelín
  • Paul Modrich
  • Steven W Matson
  • Steven R Pattishall
  • Adam Robertson
  • Adam B Robertson
  • Esther S Hong
  • G Feng
  • Pauline Funchain
  • Changill Ban
  • Alba Guarné
  • Biljana Nikolic
  • Wei Yang
  • Donald L Court
  • Grischa H Toedt
  • Arnob Dutta
  • Jennifer Wyrzykowski
  • Xin tian Li
  • Murray S Junop
  • Kazuo Negishi
  • Evangelos Thomas
  • Tohru Ogawa
  • C A Mansour
  • V Burdett
  • T Loh
  • P Modrich
  • M Reddy
  • H Liu
  • H C Tsui
  • Paul M Rindler
  • Olga Lukianova
  • Vickers Burdett
  • Sven Geisler
  • Sarah L Elliot
  • Photini Pitsikas
  • Mike O'Donnell
  • Alexandra Solovyova
  • Bernard A Connolly
  • Sanjay I Bidichandani
  • Laura M Pollard
  • Justin Mui
  • José L Barra
  • Carlos E Argaraña
  • M C Hall
  • M E Winkler
  • Mariana A Martina
  • Erin A Gibbons
  • Claus Urbanke
  • C Ban
  • Annie Yeung
  • Malgorzata M Slupska
  • Rodolfo Ghirlando
  • Xiaojian Hu
  • Santiago Ramon-Maiques
  • Yoon Bo Shim
  • Robert Ahrends
  • Bernhard Spengler
  • Draga Simic
  • Erika M Wolff
  • Dieter Kirsch
  • Dragana Mitic
  • Suhman Chung
  • Deog Su Park
  • Jasna Stanojević
  • Jelena Knezevic-Vukcevic
  • Branka Vuković-Gacić
  • Kandala V R Chary
  • Basuthkar J Rao
  • Ravi Krishnan
  • Kathryn S E Cheah
  • Michael R Volkert
  • Rory M Watt
  • Wendy Clendenin

Detail Information

Publications53

  1. ncbi Initiation of methyl-directed mismatch repair
    K G Au
    Department of Biochemistry, Duke University Medical Center, Durham, North Carolina 27710
    J Biol Chem 267:12142-8. 1992
    Escherichia coli MutH possesses an extremely weak d(GATC) endonuclease that responds to the state of methylation of the sequence (Welsh, K. M., Lu, A.-L., Clark, S., and Modrich, P. (1987) J. Biol. Chem. 262, 15624-15629)...
  2. ncbi In vitro and in vivo studies of MutS, MutL and MutH mutants: correlation of mismatch repair and DNA recombination
    Murray S Junop
    Laboratory of Molecular Biology, NIDDK, National Institutes of Health, 9000 Rockville Pike, Bldg 5, Room B1 03, Bethesda, MD 20892, USA
    DNA Repair (Amst) 2:387-405. 2003
    We have used the recently determined crystal structures of Escherichia coli (E. coli) MutS, MutL and MutH to guide construction of 47 amino-acid substitutions in these proteins and analyzed their behavior in mismatch repair and ..
  3. pmc Site-specific protein modification to identify the MutL interface of MutH
    Grischa H Toedt
    Institut für Biochemie FB 08, Justus Liebig Universitat, Heinrich Buff Ring 58, D 35392 Giessen, Germany
    Nucleic Acids Res 31:819-25. 2003
    We have mapped the region for the protein interaction site of the Escherichia coli mismatch repair protein MutH for its activator protein MutL by a site-specific protein modification approach...
  4. ncbi Cooperation and competition in mismatch repair: very short-patch repair and methyl-directed mismatch repair in Escherichia coli
    Ashok S Bhagwat
    Department of Chemistry, Wayne State University, Detroit, MI 48202, USA
    Mol Microbiol 44:1421-8. 2002
    ..data on the endonuclease that initiates VSP repair, Vsr, suggest that this protein plays a role similar to MutH (also an endonuclease) in MMR...
  5. ncbi Characterization of functional interactions among the Escherichia coli mismatch repair proteins using a bacterial two-hybrid assay
    C A Mansour
    Biology Department, Concordia University, Montreal, Que, Canada
    Mutat Res 485:331-8. 2001
    ..also show that interaction between Vsr and MutL inhibits the ability of MutL to dimerize, to interact with MutS and MutH and to mediate a previously unknown interaction between MutS and MutH...
  6. ncbi DNA mismatch repair
    Thomas A Kunkel
    Laboratory of Molecular Genetics and Laboratory of Structural Biology, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709, USA
    Annu Rev Biochem 74:681-710. 2005
    ..Emphasis is on structure-function studies of MMR proteins, on how mismatches are recognized, on the process by which the newly replicated strand is identified, and on excision of the replication error...
  7. ncbi Mutational analysis of the MutH protein from Escherichia coli
    T Loh
    Department of Pharmacology and Molecular Toxicology, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA
    J Biol Chem 276:12113-9. 2001
    Site-directed mutagenesis was performed on several areas of MutH based on the similarity of MutH and PvuII structural models...
  8. ncbi MutL-catalyzed ATP hydrolysis is required at a post-UvrD loading step in methyl-directed mismatch repair
    Adam B Robertson
    Department of Biology, University of North Carolina at Chapel Hill, NC 27599, USA
    J Biol Chem 281:19949-59. 2006
    ..MutL interacts with and modulates the activities of MutS, MutH, UvrD, and perhaps other proteins...
  9. pmc Negative regulation of mutS and mutH repair gene expression by the Hfq and RpoS global regulators of Escherichia coli K-12
    H C Tsui
    Department of Microbiology and Molecular Genetics, University of Texas Houston Medical School, 77030 1501, USA
    J Bacteriol 179:7476-87. 1997
    The MutS, MutL, and MutH proteins play major roles in several DNA repair pathways. We previously reported that the cellular amounts of MutS and MutH decreased by as much as 10-fold in stationary-phase cultures...
  10. ncbi Single-step purifications of His6-MutH, His6-MutL and His6-MutS repair proteins of escherichia coli K-12
    G Feng
    University of Texas, Houston Medical School, USA
    Biotechniques 19:956-65. 1995
    The MutS, MutH and MutL proteins mediate methyl-directed-mismatch (MDM) repair in Escherichia coli and Salmonella typhimurium...
  11. ncbi Bidirectional excision in methyl-directed mismatch repair
    M Grilley
    Department of Biochemistry, Duke University Medical Center, Durham, North Carolina 27710
    J Biol Chem 268:11830-7. 1993
    ..The extent of excision is therefore controlled by one or more components of the repair system...
  12. ncbi Methyl-directed mismatch repair is bidirectional
    D L Cooper
    Department of Biochemistry, Duke University Medical Center, Durham, North Carolina 27710
    J Biol Chem 268:11823-9. 1993
    ..Repair of both heteroduplex orientations requires MutH, MutL, MutS, DNA helicase II, SSB, and DNA polymerase III holoenzyme, but the two substrates differ with respect to ..
  13. ncbi Isolation and characterization of the Escherichia coli mutH gene product
    K M Welsh
    Department of Biochemistry, Duke University Medical Center, Durham, North Carolina 27710
    J Biol Chem 262:15624-9. 1987
    The Escherichia coli mutH gene product has been isolated in near homogeneous form using an in vitro complementation assay for DNA mismatch correction (Lu, A.-L., Clark, S., and Modrich, P. (1983) Proc. Natl. Acad. Sci. U.S.A...
  14. pmc Depletion of the cellular amounts of the MutS and MutH methyl-directed mismatch repair proteins in stationary-phase Escherichia coli K-12 cells
    G Feng
    Department of Microbiology and Molecular Genetics, University of Texas Houston Medical School 77030 1501, USA
    J Bacteriol 178:2388-96. 1996
    The MutL, MutS, and MutH proteins mediate methyl-directed mismatch (MDM) repair and help to maintain chromosome stability in Escherichia coli...
  15. pmc Protein roadblocks and helix discontinuities are barriers to the initiation of mismatch repair
    Anna Pluciennik
    Department of Biochemistry and Howard Hughes Medical Institute, Box 3711, Duke University Medical Center, Durham, NC 27710, USA
    Proc Natl Acad Sci U S A 104:12709-13. 2007
    ..Initiation of repair involves the mismatch-, MutS-, and MutL-dependent activation of MutH endonuclease, which incises the unmethylated strand at the d(GATC) sequence, with the ensuing strand break serving ..
  16. ncbi Mapping protein-protein interactions between MutL and MutH by cross-linking
    Luis Giron-Monzon
    Institut für Biochemie FB 08, Justus Liebig Universitat Giessen, D 35392 Giessen, Germany
    J Biol Chem 279:49338-45. 2004
    Strand discrimination in Escherichia coli DNA mismatch repair requires the activation of the endonuclease MutH by MutL...
  17. pmc In vivo requirement for RecJ, ExoVII, ExoI, and ExoX in methyl-directed mismatch repair
    V Burdett
    Department of Biochemistry and Howard Hughes Medical Institute, Box 3711, Duke University Medical Center, Durham, NC 27710, USA
    Proc Natl Acad Sci U S A 98:6765-70. 2001
    ..and filamentation phenotypes associated with 2-aminopurine exposure are effectively suppressed by null mutations in mutH, mutL, mutS, or uvrD/mutU, which encode activities that act upstream of the four exonucleases in the mechanism for ..
  18. ncbi DNA mismatch correction in a defined system
    R S Lahue
    Department of Biochemistry, Duke University Medical Center, Durham, NC 27710
    Science 245:160-4. 1989
    ..The Escherichia coli methyl-directed reaction has been reconstituted in a purified system consisting of MutH, MutL, and MutS proteins, DNA helicase II, single-strand DNA binding protein, DNA polymerase III holoenzyme, ..
  19. ncbi An efficient method for the preparation of long heteroduplex DNA as substrate for mismatch repair by the Escherichia coli MutHLS system
    Evangelos Thomas
    Institut fur Biochemie, Justus Liebig Universitat, Giessen, Germany
    Biol Chem 383:1459-62. 2002
    ..The application of this procedure is demonstrated for the analysis of the Escherichia coli MutHLS system...
  20. pmc Structural and functional analysis of the MutS C-terminal tetramerization domain
    Laura Manelyte
    Institut fur Biochemie, Justus Liebig Universitat, Heinrich Buff Ring 58, D 35392 Giessen, Germany
    Nucleic Acids Res 34:5270-9. 2006
    ..comparable DNA binding affinity with the tetramer-forming MutS, but is impaired in mismatch-dependent activation of MutH. Our data support the hypothesis that tetramerization of MutS is important but not essential for MutS function in ..
  21. pmc Identification of factors influencing strand bias in oligonucleotide-mediated recombination in Escherichia coli
    Xin tian Li
    Department of Biochemistry, The University of Hong Kong, 3 F Laboratory Block, Faculty of Medicine Building, 21 Sassoon Road, Pokfulam, Hong Kong SAR, PR China
    Nucleic Acids Res 31:6674-87. 2003
    ..These results demonstrate for the first time that the interplay between DNA replication and MMR has a major effect on the efficiency and strand bias of Red/SSO-mediated recombination in E.coli...
  22. pmc Enhanced levels of lambda Red-mediated recombinants in mismatch repair mutants
    Nina Costantino
    Molecular Control and Genetics Section, Gene Regulation and Chromosome Biology Laboratory, Center for Cancer Research, National Cancer Institute, Building 539, PO Box B, Frederick, MD 21702 1201, USA
    Proc Natl Acad Sci U S A 100:15748-53. 2003
    ..Our results show that Beta is the only bacteriophage function required for this level of recombination and suggest that Beta directs the ssDNA to the replication fork as it passes the target sequence...
  23. pmc Detection of protein-DNA interaction with a DNA probe: distinction between single-strand and double-strand DNA-protein interaction
    Changill Ban
    Department of Chemistry and Center for Innovative Bio physio Sensor Technology, Pusan National University, Busan 609 735, Korea
    Nucleic Acids Res 32:e110. 2004
    ..between the DNA probes with mouse Purbeta (mPurbeta), an ss-DNA binding protein, and with Escherichia coli MutH, a ds-DNA binding protein...
  24. pmc Involvement of the beta clamp in methyl-directed mismatch repair in vitro
    Anna Pluciennik
    Department of Biochemistry, Duke University Medical Center, Durham, North Carolina 27710, USA
    J Biol Chem 284:32782-91. 2009
    ..The mismatch-, MutS-, and MutL-dependent activation of MutH is unaffected by the presence or orientation of loaded beta clamp on either 3' or 5' heteroduplexes...
  25. pmc Structure of the MutL C-terminal domain: a model of intact MutL and its roles in mismatch repair
    Alba Guarné
    Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, 9000 Rockville Pike, Bethesda, MD 20892, USA
    EMBO J 23:4134-45. 2004
    ..A model of how MutL utilizes its ATPase and DNA-binding activities to mediate mismatch-dependent activation of MutH endonuclease and UvrD helicase is proposed.
  26. ncbi Comparative study of the antimutagenic potential of Vitamin E in different E. coli strains
    Biljana Nikolic
    Faculty of Biology, Laboratory for Microbiology, University of Belgrade, Studentski trg 3 II, 11000 Beograd, Serbia and Montenegro
    Mutat Res 564:31-8. 2004
    ..In Test M, a repair-proficient strain and its mismatch repair-deficient counterpart (mutH), both carrying a plasmid with microsatellite sequences, are used to measure the level of microsatellite ..
  27. pmc Mutants with temperature-sensitive defects in the Escherichia coli mismatch repair system: sensitivity to mispairs generated in vivo
    Esther S Hong
    Department of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, CA 90095, USA
    J Bacteriol 187:840-6. 2005
    ..We detected and sequenced mutations that give rise to temperature-sensitive MutS, MutL, and MutH proteins...
  28. pmc Physical and functional interactions between Escherichia coli MutL and the Vsr repair endonuclease
    Roger J Heinze
    Institut fur Biochemie, Justus Liebig Universitat, D 35392 Giessen, Germany
    Nucleic Acids Res 37:4453-63. 2009
    ..similar to that described before for the interaction between MutL and the strand discrimination endonuclease MutH of the MMR system...
  29. pmc The Escherichia coli mismatch repair protein MutL recruits the Vsr and MutH endonucleases in response to DNA damage
    Yaroslava Y Polosina
    Department of Biochemistry and Microbiology, University of Victoria, Victoria, BC, Canada
    J Bacteriol 191:4041-3. 2009
    The activities of the Vsr and MutH endonucleases of Escherichia coli are stimulated by MutL. The interaction of MutL with each enzyme is enhanced in vivo by 2-aminopurine treatment and by inactivation of the mutY gene...
  30. pmc E. coli mismatch repair acts downstream of replication fork stalling to stabilize the expanded (GAA.TTC)(n) sequence
    Rebecka L Bourn
    Department of Biochemistry and Molecular Biology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA
    Mutat Res 661:71-7. 2009
    ..While MMR destabilizes the (CTG.CAG)(n) sequence, it is involved in stabilization of the (GAA.TTC)(n) sequence. The role of MMR in triplet repeat instability therefore depends on the repeat sequence and the orientation of replication...
  31. ncbi The DNA binding activity of MutL is required for methyl-directed mismatch repair in Escherichia coli
    Adam Robertson
    Department of Biology, Curriculum in Genetics and Molecular Biology, University of North Carolina, Chapel Hill, North Carolina 27599, USA
    J Biol Chem 281:8399-408. 2006
    ..In addition, there is a modest effect on stimulation of MutH-catalyzed nicking...
  32. ncbi Mutation rate is reduced by increased dosage of mutL gene in Escherichia coli K-12
    Juan Carlos Galan
    Servicio de Microbiologia, Hospital Universitario Ramón y Cajal Unidad Asociada al CSIC Resistencia a Antibióticos y Virulencia Bacteriana and CIBER ESP, Madrid, Spain
    FEMS Microbiol Lett 275:263-9. 2007
    ..Changes in E. coli mutation frequencies derived from the gene-copy effect of mutS, mutL, mutH, uvrD, mutT, mutY, mutM, mutA, dnaE, dnaQ, and rpoS are explored...
  33. ncbi Plasmid expression of mutS, -L and/or -H gene in Escherichia coli dam cells results in strains that display reduced mutation frequency
    Daniela K Jacquelín
    Centro de Investigaciones en Quimica Biologica de Cordoba, Departamento de Quimica Biologica, Facultad de Ciencias Quimicas, Universidad Nacional de Cordoba, Haya de la Torre y Medina Allende, Ciudad Universitaria, X5000HUA, Cordoba, Argentina
    Mutat Res 637:197-204. 2008
    ..non-directed mismatch repair system; therefore, assembly of MutSLH complex at a mismatched base pair can result in MutH-mediated cleavage of GATC sites in both DNA strands...
  34. pmc Coupling distant sites in DNA during DNA mismatch repair
    Richard D Kolodner
    Ludwig Institute for Cancer Research, Department of Medicine, University of California at San Diego School of Medicine, 9500 Gilman Drive, La Jolla, CA 92093 0669, USA
    Proc Natl Acad Sci U S A 104:12953-4. 2007
  35. pmc Antagonism of ultraviolet-light mutagenesis by the methyl-directed mismatch-repair system of Escherichia coli
    H Liu
    Department of Environmental and Molecular Toxicology, Oregon State University, Corvallis, Oregon 97331 7301, USA
    Genetics 154:503-12. 2000
    ..F' lacZ targets were mated from mut(+) donors into mutH, mutL, or mutS recipients, once cells were at substantial densities, to minimize spontaneous mutation prior to ..
  36. ncbi The barrier to recombination between Escherichia coli and Salmonella typhimurium is disrupted in mismatch-repair mutants
    C Rayssiguier
    Institut Jacques Monod, Paris, France
    Nature 342:396-401. 1989
    ..for DNA sequence homology in generalized genetic recombination is greatly relaxed in bacterial mutL, mutS and mutH mutants deficient in mismatch repair...
  37. pmc Nucleotide sequence of the Escherichia coli mutH gene
    R H Grafstrom
    Nucleic Acids Res 15:3073-84. 1987
    The complete nucleotide sequence of mutH gene from E. coli has been determined. Based on the deduced amino acid sequence, the MutH protein has a molecular weight of 25...
  38. pmc Selection for high mutation rates in chemostats
    E C Cox
    Genetics 77:169-84. 1974
    ..Fitness values vary between chemostats and are strongly correlated (P < 0.001) with fluctuations in population density...
  39. pmc Selection of a mutant of Escherichia coli which has high mutation rates
    R B Helling
    J Bacteriol 96:975-80. 1968
    ..This mutation (mut-2) and the Treffers' mutation (mutT1) mapped at approximately the same position to the right of leu. Specificity studies showed that the two mutations differ in rates of mutation produced...
  40. pmc Mutator gene of Escherichia coli B
    E C Siegel
    J Bacteriol 94:38-47. 1967
    ..No suppression was found. Recombinants were prepared containing mutS1 and the Treffers mutator gene of E. coli K-12. The effect of the mutator genes appears to be additive...
  41. ncbi Cloning of mutH and identification of the gene product
    R H Grafstrom
    Gene 22:245-53. 1983
    A specialized lambda transducing phage carrying the mutH gene and several deletion derivatives of this phage were characterized by restriction enzyme analysis...
  42. pmc Structural basis for MutH activation in E.coli mismatch repair and relationship of MutH to restriction endonucleases
    C Ban
    Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    EMBO J 17:1526-34. 1998
    MutS, MutL and MutH are the three essential proteins for initiation of methyl-directed DNA mismatch repair to correct mistakes made during DNA replication in Escherichia coli...
  43. ncbi The Escherichia coli MutL protein physically interacts with MutH and stimulates the MutH-associated endonuclease activity
    M C Hall
    Department of Biology, University of North Carolina, Chapel Hill, North Carolina 27599, USA
    J Biol Chem 274:1306-12. 1999
    All possible pairwise combinations of UvrD, MutL, MutS, and MutH were tested using the yeast two-hybrid system to identify potential interactions involving mismatch repair proteins...
  44. ncbi DNA mismatch repair: molecular mechanisms and biological function
    Mark J Schofield
    Genetics and Biochemistry Branch, National Institute of Diabetes, and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
    Annu Rev Microbiol 57:579-608. 2003
    ..In addition to its role in postreplication repair, the MMR machinery serves to police homologous recombination events and acts as a barrier to genetic exchange between species...
  45. pmc Characterization of the uup locus and its role in transposon excisions and tandem repeat deletions in Escherichia coli
    M Reddy
    Centre for Cellular and Molecular Biology, Hyderabad 500 007, India
    J Bacteriol 182:1978-86. 2000
    ..It is suggested that the differential response of mini-Tn10 and Tn10 to the second category of mutations is related to the presence, respectively, of perfect and of imperfect terminal inverted repeats in them...
  46. ncbi Interaction of Escherichia coli MutS and MutL at a DNA mismatch
    M J Schofield
    Genetics and Biochemistry Branch, NIDDKD, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 276:28291-9. 2001
    ..The rate and extent of mismatch-provoked cleavage at hemimethylated GATC sites by MutH in the presence of MutS, MutL, and ATP are the same whether the mismatch and GATC sites are in cis or in trans...
  47. ncbi The datA locus predominantly contributes to the initiator titration mechanism in the control of replication initiation in Escherichia coli
    Tohru Ogawa
    Division of Biological Science, Graduate School of Science, Nagoya University, Chikusa ku, Nagoya 464 8602, Japan
    Mol Microbiol 44:1367-75. 2002
    ..These DnaA boxes may act as cores for the cooperative binding of DnaA to the entire datA region...
  48. pmc Saturation of DNA mismatch repair and error catastrophe by a base analogue in Escherichia coli
    Kazuo Negishi
    Gene Research Center, Okayama University, Okayama 700 8530, Japan
    Genetics 161:1363-71. 2002
    ..At a low dose, dP is much more mutagenic in mismatch-repair-defective mutH, mutL, and mutS strains than in a wild-type strain...
  49. pmc The Escherichia coli methyl-directed mismatch repair system repairs base pairs containing oxidative lesions
    Jennifer Wyrzykowski
    Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA
    J Bacteriol 185:1701-4. 2003
    ..on the finding that overexpression of the MutM oxidative repair protein, which repairs 8-oxoG, can suppress the mutH-dependent increase in transversion mutations...
  50. ncbi Overexpression and purification of isotopically labeled Escherichia coli MutH for NMR studies
    Arnob Dutta
    Department of Chemical Sciences, Tata Institute of Fundamental Research, Homi Bhabha Road, Colaba, Mumbai 400 005, India
    Protein Expr Purif 29:252-8. 2003
    b>MutH is one of the enzymes involved in the methyl directed -GATC-based DNA repair system...
  51. ncbi The coordinated functions of the E. coli MutS and MutL proteins in mismatch repair
    Samir Acharya
    Genetics and Molecular Biology Program, Kimmel Cancer Center BLSB 933, 233 S 10th Street, Philadelphia, PA 19107, USA
    Mol Cell 12:233-46. 2003
    ..Interaction of the MutS-MutL sliding clamp complex with MutH triggers ATP binding by MutL that enhances the endonuclease activity of MutH...
  52. ncbi Equilibrium distribution of mutators in the single fitness peak model
    Emmanuel Tannenbaum
    Harvard University, Cambridge, Massachusetts 02138, USA
    Phys Rev Lett 91:138105. 2003
    ..The repair catastrophe therefore occurs when the repair error probability exceeds the fraction of deleterious mutations. Our model also gives a quantitative estimate for the equilibrium fraction of mutators in Escherichia coli...

Research Grants4

  1. A Study of Mutation Dynamics in Biological Systems
    Emmanuel Tannenbaum; Fiscal Year: 2005
    ..This study will require the use of genomic and proteomic databases, as well as predictive methods from computational biology, in order to determine the function of the various evolving components of the biological networks under study. ..
  2. 7th Annual Midwest DNA Repair Symposium
    ASHOK BHAGWAT; Fiscal Year: 2005
    ..This Symposium will provide excellent opportunity to those scientists who are unable to attend conferences on either U.S. coast because of the distance or the cost, to participate in a major conference. ..
  3. Improving Phenylbutyrate-based Anticancer Therapy
    ASHOK BHAGWAT; Fiscal Year: 2005
    ..This work will create the tools necessary for the monitoring of tumor cells in live animals when they are treated with PB as part of combination chemotherapy. ..
  4. Discovering New Human DNA Repair Genes by Bioinformatics
    ASHOK BHAGWAT; Fiscal Year: 2007
    ..This integrated prediction-validation approach should be superior to a purely bioinformatics or a purely biochemical approach and may serve as a paradigm for searching biochemical functions in genomes of all organisms. ..