murA

Summary

Gene Symbol: murA
Description: UDP-N-acetylglucosamine 1-carboxyvinyltransferase
Alias: ECK3178, JW3156, mrbA, murZ
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. pmc Cloning and sequencing of Escherichia coli murZ and purification of its product, a UDP-N-acetylglucosamine enolpyruvyl transferase
    J L Marquardt
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115
    J Bacteriol 174:5748-52. 1992
  2. ncbi UDP-N-acetylmuramic acid (UDP-MurNAc) is a potent inhibitor of MurA (enolpyruvyl-UDP-GlcNAc synthase)
    Shehadeh Mizyed
    Department of Chemistry, Antimicrobial Research Centre, McMaster University, 1280 Main Street West, Hamilton, Ontario L8S 4M1, Canada
    Biochemistry 44:4011-7. 2005
  3. ncbi Kinetics, stoichiometry, and identification of the reactive thiolate in the inactivation of UDP-GlcNAc enolpyruvoyl transferase by the antibiotic fosfomycin
    J L Marquardt
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115
    Biochemistry 33:10646-51. 1994
  4. pmc Conditional lethal amber mutations in essential Escherichia coli genes
    Christopher D Herring
    Laboratory of Genetics, University of Wisconsin Madison, 445 Henry Mall, Madison, WI 53706, USA
    J Bacteriol 186:2673-81. 2004
  5. pmc MurA (MurZ), the enzyme that catalyzes the first committed step in peptidoglycan biosynthesis, is essential in Escherichia coli
    E D Brown
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Bacteriol 177:4194-7. 1995
  6. pmc Inhibitory mechanism of the Qβ lysis protein A2
    C A Reed
    Department of Biochemistry and Biophysics, Texas A and M University, 2128 TAMU, College Station, TX 77843 2128, USA
    Mol Microbiol 86:836-44. 2012
  7. pmc Structural basis for the interaction of the fluorescence probe 8-anilino-1-naphthalene sulfonate (ANS) with the antibiotic target MurA
    E Schonbrunn
    Department of Biochemistry and Molecular Biology, Colorado State University, Fort Collins, CO 80523, USA
    Proc Natl Acad Sci U S A 97:6345-9. 2000
  8. ncbi Characterization of a Cys115 to Asp substitution in the Escherichia coli cell wall biosynthetic enzyme UDP-GlcNAc enolpyruvyl transferase (MurA) that confers resistance to inactivation by the antibiotic fosfomycin
    D H Kim
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Biochemistry 35:4923-8. 1996
  9. pmc Cloning and identification of the Escherichia coli murB DNA sequence, which encodes UDP-N-acetylenolpyruvoylglucosamine reductase
    M J Pucci
    Department of Microbiology, Bristol Myers Squibb Company, Wallingford, Connecticut 06492 7660
    J Bacteriol 174:1690-3. 1992
  10. doi Evidence of kinetic control of ligand binding and staged product release in MurA (enolpyruvyl UDP-GlcNAc synthase)-catalyzed reactions
    Sean G Jackson
    Department of Biochemistry and Biomedical Sciences, McMaster University, 1280 Main Street West, Hamilton, Ontario L8S 4M1, Canada
    Biochemistry 48:11715-23. 2009

Scientific Experts

  • T Skarzynski
  • H Howard Xu
  • T Horii
  • Maria D F S Barbosa
  • E Z Baum
  • E Schonbrunn
  • Richard D'Ari
  • Youjun Yang
  • C A Reed
  • Ranjana Pathania
  • Sean G Jackson
  • Shan Goh
  • Hélène Barreteau
  • Anke Steinbach
  • Colin J Dunsmore
  • Danièle Joseleau-Petit
  • Paul J Berti
  • Christian D Klein
  • Anke Bachelier
  • Guy Singh
  • Peter J Petersen
  • Beth A Rasmussen
  • Tarek S Mansour
  • Pornpen Labthavikul
  • Shehadeh Mizyed
  • Gerardo D Francisco
  • Christopher D Herring
  • C T Walsh
  • Ahmed El Zoeiby
  • D H Kim
  • Joseph A DeVito
  • R Young
  • C Langlais
  • V Kuznetsov
  • E D Brown
  • Liam Good
  • Giuseppe Melacini
  • Paul Chindemi
  • Courtney Barker
  • David A Gerritsma
  • Jem Stach
  • Eric D Brown
  • Nobutaka Nakashima
  • Emilia Awuah
  • Jaroslaw M Boberek
  • Fuzhong Zhang
  • Murray S Junop
  • Rahul Das
  • Julie Lebert
  • Soumaya Zlitni
  • Fred A Capretta
  • Simon E V Phillips
  • Peter J F Henderson
  • Andreja Kovac
  • Didier Blanot
  • Matej Sova
  • Axel J Scheidig
  • Stanislav Gobec
  • Keith Miller
  • James A Garnett
  • Katy L Blake
  • William J Stubbings
  • Colin W G Fishwick
  • Joseph De Los Angeles
  • Audrey Boniface
  • Deborah J Palestrant
  • Jennifer A Leeds
  • Simon G Patching
  • Ian Chopra
  • Jean Claude Liebart
  • Juan A Ayala
  • Adam Gilbert
  • J L Marquardt
  • Kristine Svenson
  • Anatoly Severin
  • David Keeney
  • Ralf Mayer
  • Jay S Shumsky
  • Kristina M K Kutterer
  • David M Shlaes
  • William Hu
  • Amedeo A Failli
  • Girija Krishnamurthy
  • Rajiv Chopra
  • Bartosz Byczynski
  • Donald W Hughes
  • Anna Oddone
  • Frederick R Blattner
  • Zhong Li
  • Yang I Lin

Detail Information

Publications31

  1. pmc Cloning and sequencing of Escherichia coli murZ and purification of its product, a UDP-N-acetylglucosamine enolpyruvyl transferase
    J L Marquardt
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115
    J Bacteriol 174:5748-52. 1992
    The Escherichia coli gene murZ, encoding the enzyme UDP-N-acetylglucosamine enolpyruvyl transferase, has been cloned and sequenced. Identified by screening an E...
  2. ncbi UDP-N-acetylmuramic acid (UDP-MurNAc) is a potent inhibitor of MurA (enolpyruvyl-UDP-GlcNAc synthase)
    Shehadeh Mizyed
    Department of Chemistry, Antimicrobial Research Centre, McMaster University, 1280 Main Street West, Hamilton, Ontario L8S 4M1, Canada
    Biochemistry 44:4011-7. 2005
    Purified recombinant MurA (enolpyruvyl-UDP-GlcNAc synthase) overexpressed in Escherichia coli had significant amounts of UDP-MurNAc (UDP-N-acetylmuramic acid) bound after purification...
  3. ncbi Kinetics, stoichiometry, and identification of the reactive thiolate in the inactivation of UDP-GlcNAc enolpyruvoyl transferase by the antibiotic fosfomycin
    J L Marquardt
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115
    Biochemistry 33:10646-51. 1994
    ..Time-dependent inactivation of MurZ by fosfomycin was found to be greatly accelerated by the presence of cosubstrate UDP-GlcNAc but could also be ..
  4. pmc Conditional lethal amber mutations in essential Escherichia coli genes
    Christopher D Herring
    Laboratory of Genetics, University of Wisconsin Madison, 445 Henry Mall, Madison, WI 53706, USA
    J Bacteriol 186:2673-81. 2004
    ..We demonstrate the method with the genes frr, gcpE, lpxC, map, murA, ppa, and rpsA. We were unable to isolate an amber mutation in ftsZ...
  5. pmc MurA (MurZ), the enzyme that catalyzes the first committed step in peptidoglycan biosynthesis, is essential in Escherichia coli
    E D Brown
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Bacteriol 177:4194-7. 1995
    The Escherichia coli gene murZ was recently shown to encode UDP-N-acetylglucosamine enolpyruvyl transferase, which catalyzes the first committed step of peptidoglycan biosynthesis (J. L. Marquardt, D. A. Siegele, R. Kolter, and C. T...
  6. pmc Inhibitory mechanism of the Qβ lysis protein A2
    C A Reed
    Department of Biochemistry and Biophysics, Texas A and M University, 2128 TAMU, College Station, TX 77843 2128, USA
    Mol Microbiol 86:836-44. 2012
    ..present as a single copy on the surface of Qβ virion particles, was previously shown to inhibit the activity of MurA, an enzyme that catalyses the first committed step of murein biosynthesis...
  7. pmc Structural basis for the interaction of the fluorescence probe 8-anilino-1-naphthalene sulfonate (ANS) with the antibiotic target MurA
    E Schonbrunn
    Department of Biochemistry and Molecular Biology, Colorado State University, Fort Collins, CO 80523, USA
    Proc Natl Acad Sci U S A 97:6345-9. 2000
    ..ANS has been successfully used to monitor the induced-fit mechanism of MurA [UDPGlcNAc enolpyruvyltransferase (EC )], an essential enzyme for bacterial cell wall biosynthesis...
  8. ncbi Characterization of a Cys115 to Asp substitution in the Escherichia coli cell wall biosynthetic enzyme UDP-GlcNAc enolpyruvyl transferase (MurA) that confers resistance to inactivation by the antibiotic fosfomycin
    D H Kim
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Biochemistry 35:4923-8. 1996
    ..fosfomycin inhibits bacterial cell wall biosynthesis by inactivation of UDP-GlcNAc enolpyruvyl tranferase (MurA)...
  9. pmc Cloning and identification of the Escherichia coli murB DNA sequence, which encodes UDP-N-acetylenolpyruvoylglucosamine reductase
    M J Pucci
    Department of Microbiology, Bristol Myers Squibb Company, Wallingford, Connecticut 06492 7660
    J Bacteriol 174:1690-3. 1992
    ....
  10. doi Evidence of kinetic control of ligand binding and staged product release in MurA (enolpyruvyl UDP-GlcNAc synthase)-catalyzed reactions
    Sean G Jackson
    Department of Biochemistry and Biomedical Sciences, McMaster University, 1280 Main Street West, Hamilton, Ontario L8S 4M1, Canada
    Biochemistry 48:11715-23. 2009
    b>MurA (enolpyruvyl UDP-GlcNAc synthase) catalyzes the first committed step in peptidoglycan biosynthesis...
  11. doi Chemical genomics in Escherichia coli identifies an inhibitor of bacterial lipoprotein targeting
    Ranjana Pathania
    Department of Biochemistry and Biomedical Sciences, McMaster University, Hamilton, Ontario, Canada
    Nat Chem Biol 5:849-56. 2009
    ..We show that MAC13243 inhibits the function of the LolA protein and represents a new chemical probe of lipoprotein targeting in bacteria with promise as an antibacterial lead with Gram-negative selectivity...
  12. pmc Concurrent growth rate and transcript analyses reveal essential gene stringency in Escherichia coli
    Shan Goh
    Department of Cell and Molecular Biology, Karolinska Institute, Stockholm, Sweden
    PLoS ONE 4:e6061. 2009
    ....
  13. doi The unusual binding mode of cnicin to the antibacterial target enzyme MurA revealed by X-ray crystallography
    Anke Steinbach
    Pharmazeutische Chemie, Universitat Heidelberg, Im Neuenheimer Feld 364, D 69120 Heidelberg, Germany
    J Med Chem 51:5143-7. 2008
    We present the X-ray structure of the antibacterial target enzyme MurA in complex with its substrate UNAG and the sesquiterpene lactone cnicin, a potent inhibitor of the enzyme...
  14. doi Cytoplasmic steps of peptidoglycan biosynthesis
    Hélène Barreteau
    Laboratoire des Enveloppes Bactériennes et Antibiotiques, Institut de Biochimie et Biophysique Moleculaire et Cellulaire, Univ Paris Sud, Orsay, France
    FEMS Microbiol Rev 32:168-207. 2008
    ....
  15. doi 2-Aminotetralones: novel inhibitors of MurA and MurZ
    Colin J Dunsmore
    School of Chemistry and Antimicrobial Research Centre, University of Leeds, Leeds LS2 9JT, UK
    Bioorg Med Chem Lett 18:1730-4. 2008
    Several 2-aminotetralones were identified as novel inhibitors of the bacterial enzymes MurA and MurZ. A number of these inhibitors demonstrated antibacterial activity against Staphylococcus aureus and Escherichia coli with MICs in the ..
  16. pmc Unstable Escherichia coli L forms revisited: growth requires peptidoglycan synthesis
    Danièle Joseleau-Petit
    Institut Jacques Monod, 2 place Jussieu, 75251 Paris Cedex 05, France
    J Bacteriol 189:6512-20. 2007
    ..Surprisingly, however, mutant studies showed that colony formation requires d-glutamate, diaminopimelate, and MurA activity, all of which are specific to peptidoglycan synthesis...
  17. pmc An array of Escherichia coli clones over-expressing essential proteins: a new strategy of identifying cellular targets of potent antibacterial compounds
    H Howard Xu
    Department of Biological Sciences, California State University, Los Angeles, 90032, USA
    Biochem Biophys Res Commun 349:1250-7. 2006
    ..For two clones, one over-expressing FabI and the other over-expressing MurA enzymes, the host cells became 17- and 139-fold more resistant to the specific inhibitors triclosan and ..
  18. ncbi Sesquiterpene lactones are potent and irreversible inhibitors of the antibacterial target enzyme MurA
    Anke Bachelier
    Pharmazeutische und Medizinische Chemie, Saarland University, Germany
    Bioorg Med Chem Lett 16:5605-9. 2006
    ..of the sesquiterpene lactones cnicin and cynaropicrin as potent, irreversible inhibitors of the bacterial enzyme MurA. They covalently bind to the thiol group of Cys115...
  19. pmc 3,5-dioxopyrazolidines, novel inhibitors of UDP-N- acetylenolpyruvylglucosamine reductase (MurB) with activity against gram-positive bacteria
    Youjun Yang
    Wyeth Research, 401 North Middletown Rd, Pearl River, NY 10965, USA
    Antimicrob Agents Chemother 50:556-64. 2006
    ..coli MurA with 50% inhibitory concentrations (IC50s) in the range of 4.1 to 6.8 microM, 4.3 to 10.3 microM, and 6.8 to 29...
  20. pmc Isolation and characterization of a phosphonomycin-resistant mutant of Escherichia coli K-12
    P S Venkateswaran
    J Bacteriol 110:935-44. 1972
    ..Genetic studies indicate that the increased resistance toward phosphonomycin and temperature sensitivity in growth of this mutant are probably the consequences of a single mutation...
  21. ncbi Phenyl thiazolyl urea and carbamate derivatives as new inhibitors of bacterial cell-wall biosynthesis
    Gerardo D Francisco
    Chemical Sciences and Infectious Diseases, Wyeth Research, Pearl River, NY 10965, USA
    Bioorg Med Chem Lett 14:235-8. 2004
    ..Many of them demonstrated good activity against MurA and MurB and gram-positive bacteria including MRSA, VRE and PRSP...
  22. ncbi Structure and function of the Mur enzymes: development of novel inhibitors
    Ahmed El Zoeiby
    Centre de Recherche sur la Fonction, Structure et Ingénierie des Protéines, Faculte de Medecine, Pavillon Charles Eugène Marchand, Universite Laval, Ste Foy, Quebec, Canada, G1K 7P4
    Mol Microbiol 47:1-12. 2003
    ..Many studies attempted to understand the detailed mechanisms and structural features of the key enzymes MurA to MurF. Only MurA is inhibited by a known antibiotic, fosfomycin...
  23. ncbi An array of target-specific screening strains for antibacterial discovery
    Joseph A DeVito
    Department of Antimicrobial Research, Bristol Myers Squibb Company, Wilmington, DE 19880, USA
    Nat Biotechnol 20:478-83. 2002
    ..As an example of the target specificity of the approach, compounds identified in the whole-cell screen for MurA inhibitors were also found to block the biochemical function of the target when tested in vitro.
  24. pmc Development of a whole-cell assay for peptidoglycan biosynthesis inhibitors
    Maria D F S Barbosa
    Department of Antimicrobial Research, Bristol Myers Squibb Pharma, Wilmington, Delaware 19880, USA
    Antimicrob Agents Chemother 46:943-6. 2002
    ..Subsequent tests with a biochemical assay utilizing purified enzyme implicated UDP-GlcNAc enolpyruvyl transferase (MurA) as the target of Cpd1. This compound inhibits the first enzyme of the pathway in a time-dependent manner...
  25. pmc Identification and characterization of new inhibitors of the Escherichia coli MurA enzyme
    E Z Baum
    The R W Johnson Pharmaceutical Research Institute, Raritan, New Jersey 08869, USA
    Antimicrob Agents Chemother 45:3182-8. 2001
    The bacterial enzyme MurA catalyzes the transfer of enolpyruvate from phosphoenolpyruvate (PEP) to uridine diphospho-N-acetylglucosamine (UNAG), which is the first committed step of bacterial cell wall biosynthesis...
  26. ncbi Detection and characterization of a phospholactoyl-enzyme adduct in the reaction catalyzed by UDP-N-acetylglucosamine enolpyruvoyl transferase, MurZ
    E D Brown
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115
    Biochemistry 33:10638-45. 1994
    The MurZ enzyme catalyzes enolpyruvoyl transfer from phosphoenolpyruvate to the 3-OH of UDP-N-acetylglucosamine (UDP-GlcNAc) in Escherichia coli peptidoglycan biosynthesis...
  27. ncbi Stereochemical course of enzymatic enolpyruvyl transfer and catalytic conformation of the active site revealed by the crystal structure of the fluorinated analogue of the reaction tetrahedral intermediate bound to the active site of the C115A mutant of Mu
    T Skarzynski
    Glaxo Wellcome Research and Development, Medicines Research Centre, Stevenage, Hertfordshire, United Kingdom SG1 2NY
    Biochemistry 37:2572-7. 1998
    b>MurA (UDP-GlcNAc enolpyruvyl transferase), the first enzyme in bacterial peptidoglycan biosynthesis, catalyzes the enolpyruvyl transfer from phosphoenolpyruvate (PEP) to the 3'-OH of UDP-GlcNAc by an addition-elimination mechanism that ..
  28. ncbi Structure of UDP-N-acetylglucosamine enolpyruvyl transferase, an enzyme essential for the synthesis of bacterial peptidoglycan, complexed with substrate UDP-N-acetylglucosamine and the drug fosfomycin
    T Skarzynski
    Glaxo Wellcome Research and Development, Medicines Research Centre, Stevenage, UK ts
    Structure 4:1465-74. 1996
    UDP-N-acetylglucosamine enolpyruvyl transferase (MurA), catalyses the first committed step of bacterial cell wall biosynthesis and is a target for the antibiotic fosfomycin...
  29. ncbi Analysis of fluoromethyl group chirality establishes a common stereochemical course for the enolpyruvyl transfers catalyzed by EPSP synthase and UDP-GlcNAc enolpyruvyl transferase
    D H Kim
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Biochemistry 35:5435-40. 1996
    ..Comparison of these data with prior analogous work on the MurA reaction [Kim, D.H., Lees, W.J., & Walsh, C. T. (1995) J. Am. Chem. Soc...
  30. pmc Cloning and sequencing of an Escherichia coli gene, nlp, highly homologous to the ner genes of bacteriophages Mu and D108
    Y L Choi
    Department of Agricultural Chemistry, Kyoto University, Japan
    J Bacteriol 171:5222-5. 1989
    ..The amino-terminal region of Nlp deduced from the complete open reading frame contained a presumed DNA-binding region. The nlp gene was located at 69.3 min on the E. coli genetic map...
  31. pmc Emergence of fosfomycin-resistant isolates of Shiga-like toxin-producing Escherichia coli O26
    T Horii
    Department of Bacteriology, Nagoya University School of Medicine, Japan
    Antimicrob Agents Chemother 43:789-93. 1999
    ..Cloning, sequencing, and Northern blot analysis demonstrated that the transcriptional level of the murA gene encoding UDP-N-acetylglucosamine enolpyruvoyl transferase in these isolates was greater than that in E...