mechanosensitive channel

Summary

Gene Symbol: mechanosensitive channel
Description: mechanosensitive channel protein, small conductance
Alias: ECK2920, JW2891, yggB
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. doi The tension-transmitting 'clutch' in the mechanosensitive channel MscS
    Vladislav Belyy
    Department of Biology, University of Maryland College Park, College Park, Maryland, USA
    Nat Struct Mol Biol 17:451-8. 2010
  2. pmc 2,2,2-Trifluoroethanol changes the transition kinetics and subunit interactions in the small bacterial mechanosensitive channel MscS
    Bradley Akitake
    Department of Biology, University of Maryland, College Park, Maryland
    Biophys J 92:2771-84. 2007
  3. ncbi Mechanosensitive ion channels: molecules of mechanotransduction
    Boris Martinac
    School of Medicine and Pharmacology, QEII Medical Centre, University of Western Australia, Crawley, WA 6009, Australia
    J Cell Sci 117:2449-60. 2004
  4. pmc Mechanosensitive channel MscS in the open state: modeling of the transition, explicit simulations, and experimental measurements of conductance
    Andriy Anishkin
    Department of Biology, University of Maryland, College Park, MD 20742, USA
    J Gen Physiol 132:67-83. 2008
  5. pmc Three-dimensional architecture of membrane-embedded MscS in the closed conformation
    Valeria Vásquez
    Department of Molecular Physiology and Biological Physics, University of Virginia, Charlottesville, VA 22908, USA
    J Mol Biol 378:55-70. 2008
  6. ncbi The closed structure of the MscS mechanosensitive channel. Cross-linking of single cysteine mutants
    Samantha Miller
    Department of Molecular and Cell Biology, Institute of Medical Sciences, University of Aberdeen, Foresterhill, Aberdeen AB25 2ZD, United Kingdom
    J Biol Chem 278:32246-50. 2003
  7. ncbi Straightening and sequential buckling of the pore-lining helices define the gating cycle of MscS
    Bradley Akitake
    Department of Biology, University of Maryland, Building 144, College Park, Maryland 20742, USA
    Nat Struct Mol Biol 14:1141-9. 2007
  8. pmc Characterization of the resting MscS: modeling and analysis of the closed bacterial mechanosensitive channel of small conductance
    Andriy Anishkin
    Department of Biology, University of Maryland, College Park, Maryland, USA
    Biophys J 94:1252-66. 2008
  9. ncbi Gating the bacterial mechanosensitive channels: MscS a new paradigm?
    Michelle D Edwards
    School of Medical Sciences, University of Aberdeen, Foresterhill, Aberdeen AB25 2ZD, Scotland, UK
    Curr Opin Microbiol 7:163-7. 2004
  10. pmc Gating of the large mechanosensitive channel in situ: estimation of the spatial scale of the transition from channel population responses
    Chien Sung Chiang
    Department of Biology, University of Maryland College Park, Maryland 20742, USA
    Biophys J 86:2846-61. 2004

Scientific Experts

  • P Blount
  • M D Edwards
  • James H Naismith
  • Daniel Balleza
  • S Sukharev
  • Philip C Biggin
  • A G Macdonald
  • B Martinac
  • F Bezanilla
  • Ann Finney Batiza
  • Andriy Anishkin
  • Takeshi Nomura
  • Marcos Sotomayor
  • Ian R Booth
  • Valeria Vásquez
  • Bradley Akitake
  • Kishore Kamaraju
  • Samantha Miller
  • Eduardo Perozo
  • Douglas C Rees
  • Masahiro Sokabe
  • Klaus Schulten
  • Vladislav Belyy
  • Ben Corry
  • Pavel Strop
  • Hannah R Malcolm
  • Randal B Bass
  • Jeffrey Y Lai
  • Ulrike Schumann
  • Wendy Bartlett
  • Kenjiro Yoshimura
  • Piotr Koprowski
  • Annette C Hurst
  • Hiroaki Machiyama
  • Pierre Alexandre Boucher
  • Naili Liu
  • Wenjian Wang
  • Chris S Gandhi
  • D Marien Cortes
  • Akiko Rasmussen
  • Steven A Spronk
  • Therese Schroeder-Sheker
  • Elizabeth S Haswell
  • Taira Vora
  • Joost H A Folgering
  • Wojciech Grajkowski
  • Thom Nguyen
  • Ching Kung
  • George Shapovalov
  • Sheilagh Clarkson
  • Andrzej Kubalski
  • Chien Sung Chiang
  • I R Booth
  • C R Calladine
  • Christopher D Pivetti
  • Neil R Stokes
  • Navid Bavi
  • Charles D Cox
  • Subramanian Chandrasekaran
  • Joshua A Maurer
  • Debra McLaggan
  • Anna Kloda
  • Kuniyuki Okada
  • Paul C Moe
  • Michelle M Moore
  • Yuezhou Li
  • Jens T Kaiser
  • Yan Shuen Poon
  • Ian Rowe
  • A Kubalski
  • Hitoshi Tatsumi
  • Philip A Gottlieb
  • Béla Joós
  • Catherine E Morris
  • N Levina
  • Emma L Morrison
  • Susan S Black
  • Julio Cordero-Morales
  • P Louis
  • Changjiang Dong
  • Benoit Roux
  • Ben de Kruijff
  • Hiro Furukawa
  • Daniela Schauer
  • Robin E J Spelbrink
  • Tim Rasmussen
  • J Antoinette Killian
  • Donald E Elmore
  • Trudy A van der Straaten
  • Umberto Ravaioli

Detail Information

Publications75

  1. doi The tension-transmitting 'clutch' in the mechanosensitive channel MscS
    Vladislav Belyy
    Department of Biology, University of Maryland College Park, College Park, Maryland, USA
    Nat Struct Mol Biol 17:451-8. 2010
    Under prolonged stimulation, the mechanosensitive channel MscS of Escherichia coli enters a tension-insensitive inactivated state...
  2. pmc 2,2,2-Trifluoroethanol changes the transition kinetics and subunit interactions in the small bacterial mechanosensitive channel MscS
    Bradley Akitake
    Department of Biology, University of Maryland, College Park, Maryland
    Biophys J 92:2771-84. 2007
    ..One of these was the homo-heptameric stretch-activated mechanosensitive channel of small conductance (MscS), a ubiquitous component of the bacterial turgor-regulation system...
  3. ncbi Mechanosensitive ion channels: molecules of mechanotransduction
    Boris Martinac
    School of Medicine and Pharmacology, QEII Medical Centre, University of Western Australia, Crawley, WA 6009, Australia
    J Cell Sci 117:2449-60. 2004
    ..Moreover, cloning and sequencing of MS channels from several species has provided insights into their evolution, their physiological functions in prokaryotes and eukaryotes, and their potential roles in the pathology of disease...
  4. pmc Mechanosensitive channel MscS in the open state: modeling of the transition, explicit simulations, and experimental measurements of conductance
    Andriy Anishkin
    Department of Biology, University of Maryland, College Park, MD 20742, USA
    J Gen Physiol 132:67-83. 2008
    ....
  5. pmc Three-dimensional architecture of membrane-embedded MscS in the closed conformation
    Valeria Vásquez
    Department of Molecular Physiology and Biological Physics, University of Virginia, Charlottesville, VA 22908, USA
    J Mol Biol 378:55-70. 2008
    The mechanosensitive channel of small conductance (MscS) is part of a coordinated response to osmotic challenges in Escherichia coli...
  6. ncbi The closed structure of the MscS mechanosensitive channel. Cross-linking of single cysteine mutants
    Samantha Miller
    Department of Molecular and Cell Biology, Institute of Medical Sciences, University of Aberdeen, Foresterhill, Aberdeen AB25 2ZD, United Kingdom
    J Biol Chem 278:32246-50. 2003
    ..The data are also consistent with significant flexibility of the cytoplasmic domain...
  7. ncbi Straightening and sequential buckling of the pore-lining helices define the gating cycle of MscS
    Bradley Akitake
    Department of Biology, University of Maryland, Building 144, College Park, Maryland 20742, USA
    Nat Struct Mol Biol 14:1141-9. 2007
    We describe a mechanism connecting the adaptive behavior of the bacterial mechanosensitive channel MscS to the flexibility of the pore-lining helix TM3...
  8. pmc Characterization of the resting MscS: modeling and analysis of the closed bacterial mechanosensitive channel of small conductance
    Andriy Anishkin
    Department of Biology, University of Maryland, College Park, Maryland, USA
    Biophys J 94:1252-66. 2008
    ..From this closed-resting conformation, the TM3 helices must expand to allow for channel opening...
  9. ncbi Gating the bacterial mechanosensitive channels: MscS a new paradigm?
    Michelle D Edwards
    School of Medical Sciences, University of Aberdeen, Foresterhill, Aberdeen AB25 2ZD, Scotland, UK
    Curr Opin Microbiol 7:163-7. 2004
    ..Discovery of the gene for a second class of mechanosensitive channel, MscS, and its subsequent crystallisation, has provided a new paradigm for mechanosensation, enabling a ..
  10. pmc Gating of the large mechanosensitive channel in situ: estimation of the spatial scale of the transition from channel population responses
    Chien Sung Chiang
    Department of Biology, University of Maryland College Park, Maryland 20742, USA
    Biophys J 86:2846-61. 2004
    ..Using the all-point histogram method and calibrating tension against the threshold for the small mechanosensitive channel (MscS) in each patch, we found that the distribution of channels among the subconducting states is ..
  11. pmc Water dynamics and dewetting transitions in the small mechanosensitive channel MscS
    Andriy Anishkin
    Biology Department, University of Maryland, College Park, Maryland, USA
    Biophys J 86:2883-95. 2004
    ..The recently solved heptameric structure of the small mechanosensitive channel of Escherichia coli, MscS, has revealed a relatively wide (7-15 A) yet highly hydrophobic transmembrane ..
  12. pmc Two families of mechanosensitive channel proteins
    Christopher D Pivetti
    Division of Biology, University of California San Diego, La Jolla, California 92093 0116, USA
    Microbiol Mol Biol Rev 67:66-85, table of contents. 2003
    ..The presence of a conserved residue pattern for the putative channel-forming TMSs in the MscL and MscS family proteins suggests a common structural organization, gating mechanism, and evolutionary origin...
  13. pmc Gating transitions in bacterial ion channels measured at 3 microns resolution
    George Shapovalov
    Division of Biology, California Institute of Technology, Pasadena, CA 91125, USA
    J Gen Physiol 124:151-61. 2004
    ....
  14. pmc Molecular dynamics study of gating in the mechanosensitive channel of small conductance MscS
    Marcos Sotomayor
    Department of Physics, University of Illinois at Urbana Champaign, and Beckman Institute for Advanced Science and Technology, Urbana, Illinois
    Biophys J 87:3050-65. 2004
    ..MscS, the mechanosensitive channel of small conductance, is found in the inner membrane of Escherichia coli and its crystallographic ..
  15. pmc The "dashpot" mechanism of stretch-dependent gating in MscS
    Bradley Akitake
    Department of Biology, University of Maryland, College Park, MD 20742, USA
    J Gen Physiol 125:143-54. 2005
    The crystal structure of the small conductance mechanosensitive channel (MscS) has been an invaluable tool in the search for the gating mechanism, however many functional aspects of the channel remain unsettled...
  16. pmc Surface changes of the mechanosensitive channel MscS upon its activation, inactivation, and closing
    Wojciech Grajkowski
    Nencki Institute of Experimental Biology, 02 093 Warsaw, Poland
    Biophys J 88:3050-9. 2005
    MscS is a bacterial mechanosensitive channel that shows voltage dependence. The crystal structure of MscS revealed that the channel is a homoheptamer with a large chamber on the intracellular site...
  17. ncbi Pivotal role of the glycine-rich TM3 helix in gating the MscS mechanosensitive channel
    Michelle D Edwards
    School of Medical Sciences, University of Aberdeen, Institute of Medical Sciences, Foresterhill, Aberdeen, AB25 2ZD, UK
    Nat Struct Mol Biol 12:113-9. 2005
    The crystal structure of an open form of the Escherichia coli MscS mechanosensitive channel was recently solved. However, the conformation of the closed state and the gating transition remain uncharacterized...
  18. pmc Electrostatic properties of the mechanosensitive channel of small conductance MscS
    Marcos Sotomayor
    Department of Physics, Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana Champaign, USA
    Biophys J 90:3496-510. 2006
    The mechanosensitive channel of small conductance (MscS) belongs to a family of membrane proteins that are gated in response to changes in membrane tension, thereby protecting the cell from hypo-osmotic shock...
  19. pmc Voltage-dependent hydration and conduction properties of the hydrophobic pore of the mechanosensitive channel of small conductance
    Steven A Spronk
    Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, USA
    Biophys J 90:3555-69. 2006
    ..This mechanism has been implicated in the gating of several channels, including the mechanosensitive channel of small conductance (MscS)...
  20. ncbi MscS-like proteins control plastid size and shape in Arabidopsis thaliana
    Elizabeth S Haswell
    Division of Biology, 156 29, California Institute of Technology, 1200 E California Blvd, Pasadena, California 91125, USA
    Curr Biol 16:1-11. 2006
    ..MscS-like proteins are found widely in bacterial and archaeal species and have also been identified in fission yeast and plants. None of the eukaryotic members of the family have yet been characterized...
  21. ncbi C termini of the Escherichia coli mechanosensitive ion channel (MscS) move apart upon the channel opening
    Piotr Koprowski
    Department of Cell Biology, Nencki Institute of Experimental Biology 3, Pasteur Street, 02 093 Warsaw, Poland
    J Biol Chem 278:11237-45. 2003
    Heptameric YggB is a mechanosensitive ion channel (MscS) from the inner membrane of Escherichia coli...
  22. ncbi A patch-clamp study of ion channels of inner and outer membranes and of contact zones of E. coli, fused into giant liposomes. Pressure-activated channels are localized in the inner membrane
    C Berrier
    Laboratoire des Biomembranes, URA CNRS 1116, Orsay, France
    FEBS Lett 259:27-32. 1989
    ..The outer membrane contained pressure-insensitive channels with large conductances and long opening and closing times which are likely to be porins. Large channels were also observed in contact zones...
  23. pmc Protection of Escherichia coli cells against extreme turgor by activation of MscS and MscL mechanosensitive channels: identification of genes required for MscS activity
    N Levina
    Department of Molecular and Cell Biology, University of Aberdeen, Institute of Medical Sciences, Foresterhill, Aberdeen AB25 2ZD, UK
    EMBO J 18:1730-7. 1999
    ..Here, we report the characterization of a new gene family required for MscS function, YggB and KefA, which has enabled a rigorous test of the role of the channels...
  24. ncbi Managing hypoosmotic stress: aquaporins and mechanosensitive channels in Escherichia coli
    I R Booth
    Department of Molecular and Cell Biology, University of Aberdeen, Institute of Medical Sciences, Foresterhill, Aberdeen, AB25 2ZD, UK
    Curr Opin Microbiol 2:166-9. 1999
    ..The isolation of specific mutations and the structural studies on MscL now pave the way to a molecular understanding of the mechanism of activation of mechanosensitive channels...
  25. pmc Purification of the small mechanosensitive channel of Escherichia coli (MscS): the subunit structure, conduction, and gating characteristics in liposomes
    Sergei Sukharev
    Department of Biology, University of Maryland, College Park, Maryland 20742, USA
    Biophys J 83:290-8. 2002
    The small mechanosensitive channel, MscS, is a part of the turgor-driven solute efflux system that protects bacteria from lysis in the event of osmotic downshift...
  26. pmc Ionic regulation of MscK, a mechanosensitive channel from Escherichia coli
    Yuezhou Li
    Department of Physiology, University of Texas Southwestern Medical Center, Dallas, TX 75390 9040, USA
    EMBO J 21:5323-30. 2002
    Three gene products that form independent mechanosensitive channel activities have been identified in Escherichia coli. Two of these, MscL and MscS, play a vital role in allowing the cell to survive acute hypotonic stress...
  27. ncbi Crystal structure of Escherichia coli MscS, a voltage-modulated and mechanosensitive channel
    Randal B Bass
    Division of Chemistry and Chemical Engineering, Biochemistry Option, Howard Hughes Medical Institute, Mail Code 114 96, California Institute of Technology, Pasadena, CA 91125, USA
    Science 298:1582-7. 2002
    The mechanosensitive channel of small conductance (MscS) responds both to stretching of the cell membrane and to membrane depolarization. The crystal structure at 3...
  28. pmc The structure of an open form of an E. coli mechanosensitive channel at 3.45 A resolution
    Wenjian Wang
    Centre for Biomolecular Sciences, The North Haugh, University of St Andrews, KY16 9ST, Scotland, UK
    Science 321:1179-83. 2008
    ..The Escherichia coli mechanosensitive channel, MscS, opens to allow rapid ion efflux, relieving the turgor pressure that would otherwise destroy the ..
  29. doi The membrane lateral pressure-perturbing capacity of parabens and their effects on the mechanosensitive channel directly correlate with hydrophobicity
    Kishore Kamaraju
    Department of Biology, University of Maryland, College Park, Maryland 20742, USA
    Biochemistry 47:10540-50. 2008
    ..Here we describe effects of ethyl, propyl, and butyl parabens on the gating of the bacterial mechanosensitive channel of small conductance (MscS) and compare them with the surface activity and lateral pressure changes ..
  30. doi Conserved motifs in mechanosensitive channels MscL and MscS
    Daniel Balleza
    Laboratory of Molecular Biology, University of Wisconsin, 1525 Linden Drive, Madison, WI, 53706, USA
    Eur Biophys J 38:1013-27. 2009
    ..We found that the conservation in MscL homologs is high for TM1 and TM2 in the three domains of life. The conservation in MscS homologs is high only for TM3 in Bacteria and Archaea...
  31. ncbi Functional design of bacterial mechanosensitive channels. Comparisons and contrasts illuminated by random mutagenesis
    Kuniyuki Okada
    Department of Physiology, University of Texas Southwestern Medical Center, Dallas, Texas 75235 9040, USA
    J Biol Chem 277:27682-8. 2002
    ..Previously, it was shown that eliminating the expression of Escherichia coli yggB removed a major portion of MscS activity. YggB is distinct from MscL by having no obvious structural similarity...
  32. ncbi Analysis of the kefA2 mutation suggests that KefA is a cation-specific channel involved in osmotic adaptation in Escherichia coli
    Debra McLaggan
    Department of Molecular and Cell Biology, University of Aberdeen, Institute of Medical Sciences, Foresterhill, UK
    Mol Microbiol 43:521-36. 2002
    ..organization of the KefA protein and the phenotype of a missense mutation, kefA, which affects the KefA mechanosensitive channel. The altered function of the channel is manifest through increased sensitivity to K+ during growth at low ..
  33. pmc Ion conduction through MscS as determined by electrophysiology and simulation
    Marcos Sotomayor
    Department of Physics, University of Illinois at Urbana Champaign, and Beckman Institute for Advanced Science and Technology, Urbana, Illinois, USA
    Biophys J 92:886-902. 2007
    The mechanosensitive channel of small conductance (MscS) is a membrane protein thought to act as a safety valve in bacteria, regulating the release of ions and small solutes when gated by membrane tension under challenging osmotic ..
  34. ncbi The conserved carboxy-terminus of the MscS mechanosensitive channel is not essential but increases stability and activity
    Ulrike Schumann
    School of Medical Sciences, University of Aberdeen, Institute of Medical Sciences, Foresterhill, Aberdeen AB25 2ZD, UK
    FEBS Lett 572:233-7. 2004
    The Escherichia coli MscS mechanosensitive channel protein has a distinct domain structure that terminates in a conserved seven-strand beta barrel...
  35. ncbi Structure and mechanism in prokaryotic mechanosensitive channels
    Eduardo Perozo
    Department of Molecular Physiology and Biological Physics, University of Virginia, Charlottesville, VA 22908, USA
    Curr Opin Struct Biol 13:432-42. 2003
    ..Recent functional, structural and dynamic data offer fresh insights into the molecular basis of gating for these membrane proteins...
  36. ncbi Brownian dynamics investigation into the conductance state of the MscS channel crystal structure
    Taira Vora
    Department of Theoretical Physics, Research School of Physical Sciences, The Australian National University, Canberra, A C T 0200, Australia
    Biochim Biophys Acta 1758:730-7. 2006
    We suggest that the crystal structure of the mechanosensitive channel of small conductance is in a minimally conductive state rather than being fully activated...
  37. pmc Two types of mechanosensitive channels in the Escherichia coli cell envelope: solubilization and functional reconstitution
    S I Sukharev
    Laboratory of Molecular Biology, University of Wisconsin, Madison 53706
    Biophys J 65:177-83. 1993
    ....
  38. ncbi Bacterial mechanosensitive channels: integrating physiology, structure and function
    P Blount
    Dept of Physiology, University of Texas Southwestern Medical Centre, Dallas, TX 75235 9040, USA
    Trends Microbiol 7:420-4. 1999
    ..Mutagenesis of one of these channels, combined with structural information derived from X-ray crystallography, has given the first clues of how a mechanosensitive channel senses and responds to membrane tension.
  39. pmc Open and shut: crystal structures of the dodecylmaltoside solubilized mechanosensitive channel of small conductance from Escherichia coli and Helicobacter pylori at 4.4 Å and 4.1 Å resolutions
    Jeffrey Y Lai
    Division of Chemistry and Chemical Engineering, Howard Hughes Medical Institute, California Institute of Technology, Pasadena, California 91125, USA
    Protein Sci 22:502-9. 2013
    The mechanosensitive channel of small conductance (MscS) contributes to the survival of bacteria during osmotic downshock by transiently opening large diameter pores for the efflux of cellular contents before the membrane ruptures...
  40. pmc The mechanosensitive channel of small conductance (MscS) functions as a Jack-in-the box
    Hannah R Malcolm
    Department of Chemistry, Washington University in St Louis, MO 63130, USA Department of Physiology, University of Texas Southwestern Medical Center, Dallas, TX 76390, USA
    Biochim Biophys Acta 1848:159-66. 2015
    Phenotypical analysis of the lipid interacting residues in the closed state of the mechanosensitive channel of small conductance (MscS) from Escherichia coli (E...
  41. doi Unidirectional incorporation of a bacterial mechanosensitive channel into liposomal membranes
    Takeshi Nomura
    Molecular Cardiology and Biophysics Division Mechanosensory Biophysics Laboratory, Victor Chang Cardiac Research Institute, Darlinghurst, New South Wales, Australia Department of Physical Therapy, Faculty of Rehabilitation, Graduate School of Health Sciences, Kyushu Nutrition Welfare University, Kitakyushu, Japan St Vincent s Clinical School, The University of New South Wales, Sydney, New South Wales, Australia and Mechanobiology Laboratory, Nagoya University Graduate School of Medicine, Nagoya, Japan
    FASEB J 29:4334-45. 2015
    The bacterial mechanosensitive channel of small conductance (MscS) plays a crucial role in the protection of bacterial cells against hypo-osmotic shock...
  42. pmc Voltage-Dependent Inactivation of MscS Occurs Independently of the Positively Charged Residues in the Transmembrane Domain
    Takeshi Nomura
    International Cooperative Research Project ICORP Solution Oriented Research for Science and Technology SORST, Cell Mechanosensing Project, Japan Science and Technology Agency, Nagoya 466 8550, Japan Department of Rehabilitation, Kyushu Nutrition Welfare University, Kitakyushu 800 029, Japan
    Biomed Res Int 2016:2401657. 2016
    MscS (mechanosensitive channel of small conductance) is ubiquitously found among bacteria and plays a major role in avoiding cell lysis upon rapid osmotic downshock...
  43. pmc Structural changes in the cytoplasmic domain of the mechanosensitive channel MscS during opening
    Hiroaki Machiyama
    Department of Physiology, Nagoya University Graduate School of Medicine, Nagoya, Japan
    Biophys J 97:1048-57. 2009
    The bacterial mechanosensitive channel MscS forms a homoheptamer of subunits composed of a transmembrane (TM) domain and a large cytoplasmic (CP) domain...
  44. pmc Bacterial mechanosensitive channels--MscS: evolution's solution to creating sensitivity in function
    James H Naismith
    Biomedical Sciences Research Complex, The North Haugh, The University of St Andrews, Fife KY16 9ST, United Kingdom
    Annu Rev Biophys 41:157-77. 2012
    The discovery of mechanosensing channels has changed our understanding of bacterial physiology. The mechanosensitive channel of small conductance (MscS) is perhaps the most intensively studied of these channels...
  45. pmc Mechanosensitive closed-closed transitions in large membrane proteins: osmoprotection and tension damping
    Pierre Alexandre Boucher
    Institut de Physique, Ottawa Carleton Campus de l Université d Ottawa, Ottawa, Ontario, Canada
    Biophys J 97:2761-70. 2009
    ..One spandex species could not excel as both first line of defense for osmovalve partners and tension damper. Possible interactions among MS closed-closed and closed-open transitions are discussed for MscS- and MscL-like proteins...
  46. doi Biochemistry. Opening the molecular floodgates
    Chris S Gandhi
    Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, CA 91125, USA
    Science 321:1166-7. 2008
  47. ncbi Identification, molecular cloning and sequence analysis of a gene cluster encoding the class II fructose 1,6-bisphosphate aldolase, 3-phosphoglycerate kinase and a putative second glyceraldehyde 3-phosphate dehydrogenase of Escherichia coli
    P R Alefounder
    Department of Biochemistry, University of Cambridge, UK
    Mol Microbiol 3:723-32. 1989
    ..coli and which maps elsewhere on the chromosome. The products, if any, of several other open reading frames remain to be identified...
  48. ncbi [Bacterial ion canals]
    A Kubalski
    Zakład Biologii Komórki, Instytut Biologii Doświadczalnej im M Nenckiego PAN w Warszawie
    Postepy Hig Med Dosw 54:317-27. 2000
    ..with structural information derived from X-ray crystalography, have brought the knowledge of how a mechanosensitive channel senses membrane tension...
  49. ncbi Mechanosensitive channels in prokaryotes
    B Martinac
    Department of Pharmacology, University of Western Australia, Nedlands, Australia
    Cell Physiol Biochem 11:61-76. 2001
    ..Therefore, throughout this review prokaryotic MS ion channels are referred to as MS channels rather than MS ion channels...
  50. pmc Gating the bacterial mechanosensitive channel MscL invivo
    Ann Finney Batiza
    Laboratory of Molecular Biology, University of Wisconsin, Madison, WI 53706, USA
    Proc Natl Acad Sci U S A 99:5643-8. 2002
    b>YggB and MscL are the major mechanosensitive channels in Escherichia coli, and each can rescue the double knockout mutant from osmotic downshock...
  51. pmc The pathway and spatial scale for MscS inactivation
    Kishore Kamaraju
    Department of Biology, University of Maryland, College Park, MD 20742, USA
    J Gen Physiol 138:49-57. 2011
    The mechanosensitive channel of small conductance (MscS) is a bacterial tension-driven osmolyte release valve with homologues in many walled eukaryotic organisms...
  52. ncbi Mechanosensitive channels of bacteria and archaea share a common ancestral origin
    Anna Kloda
    Department of Pharmacology, QEII Medical Center, The University of Western Australia, Crawley
    Eur Biophys J 31:14-25. 2002
    ..TM1 structural motifs and showed a high degree of se quence and secondary structure conservation with MscS (YggB) homologues...
  53. ncbi Structural biology. Force and voltage sensors in one structure
    Francisco Bezanilla
    Department of Physiology, University of California, Los Angeles, CA 90095, USA
    Science 298:1562-3. 2002
  54. pmc State-stabilizing Interactions in Bacterial Mechanosensitive Channel Gating and Adaptation
    Andriy Anishkin
    Department of Biology, University of Maryland, College Park, Maryland 20742, USA
    J Biol Chem 284:19153-7. 2009
    ..We discuss physical factors that may direct the transitions and stabilize main functional states in these channels...
  55. doi Concentration dependent effect of GsMTx4 on mechanosensitive channels of small conductance in E. coli spheroplasts
    Annette C Hurst
    Molecular Biophysics Laboratory, School of Biomedical Sciences, University of Queensland, St Lucia, QLD, 4072, Australia
    Eur Biophys J 38:415-25. 2009
    ..In contrast, application of peptide at concentrations of 12 and 20 microM facilitated prokaryotic MS channel opening by increasing the pressure sensitivity...
  56. pmc A structural mechanism for MscS gating in lipid bilayers
    Valeria Vásquez
    Department of Molecular Physiology and Biological Physics, University of Virginia, Charlottesville, VA 22908, USA
    Science 321:1210-4. 2008
    The mechanosensitive channel of small conductance (MscS) is a key determinant in the prokaryotic response to osmotic challenges...
  57. ncbi Cylindrical channels from concave helices
    C R Calladine
    Science 299:661-2. 2003
  58. pmc Engineering covalent oligomers of the mechanosensitive channel of large conductance from Escherichia coli with native conductance and gating characteristics
    Joost H A Folgering
    Department of Biochemistry, University of Groningen, Nijenborgh 4, 9747 AG, Groningen, The Netherlands
    Protein Sci 14:2947-54. 2005
    ..construct for making single substitutions per channel and to determine the quaternary structure of the mechanosensitive channel MscL from Escherichia coli, covalent oligomers (monomer to hexamer) were engineered by gene fusion; up to ..
  59. ncbi Prokaryotic mechanosensitive channels
    Pavel Strop
    California Institute of Technology, Pasadena, California 91125, USA
    Adv Protein Chem 63:177-209. 2003
  60. ncbi The effects of parabens on the mechanosensitive channels of E. coli
    Thom Nguyen
    School of Medicine and Pharmacology, QEII Medical Centre, The University of Western Australia, Crawley, WA 6009, Australia
    Eur Biophys J 34:389-95. 2005
    ..In this study we examined the effects of ethyl and propyl paraben, on gating of the E. coli mechanosensitive channel of large conductance (MscL) reconstituted into azolectin liposomes...
  61. ncbi Structural plasticity in MS channels
    Boris Martinac
    Nat Struct Mol Biol 12:104-5. 2005
  62. pmc Lipid-protein interaction of the MscS mechanosensitive channel examined by scanning mutagenesis
    Takeshi Nomura
    ICORP SORST, Cell Mechanosensing, Japan Science and Technology Agency, Nagoya, Japan
    Biophys J 91:2874-81. 2006
    The mechanosensitive channel of small conductance (MscS) is a bacterial mechanosensitive channel that opens in response to rapid hypoosmotic stress...
  63. ncbi Channels in microbes: so many holes to fill
    Ching Kung
    Laboratory of Molecular Biology and Department of Genetics, University of Wisconsin, Madison, WI, USA
    Mol Microbiol 53:373-80. 2004
    ..The intellectual and technical schisms between 'neuro' and 'micro' biology must be bridged before we know how we became so smart, and whether microbes are just as smart...
  64. ncbi Regulating osmosensing
    Sheilagh Clarkson
    Nat Rev Microbiol 2:86-7. 2004
  65. ncbi An optimized purification and reconstitution method for the MscS channel: strategies for spectroscopical analysis
    Valeria Vásquez
    Department of Molecular Physiology and Biological Physics, University of Virginia, Charlottesville, Virginia 22908, USA
    Biochemistry 46:6766-73. 2007
    The mechanosensitive channel of small conductance (MscS) plays a critical role in the osmoregulation of prokaryotic cells...
  66. ncbi Bacterial mechanosensitive channels: experiment and theory
    Ben Corry
    School of Biomedical, Biomolecular and Chemical Sciences, University of Western Australia, Crawley, WA 6008, Australia
    Biochim Biophys Acta 1778:1859-70. 2008
    ....
  67. ncbi The role of tryptophan residues in the function and stability of the mechanosensitive channel MscS from Escherichia coli
    Akiko Rasmussen
    School of Medical Sciences, University of Aberdeen, Institute of Medical Sciences, Foresterhill, Aberdeen, AB25 2ZD, UK
    Biochemistry 46:10899-908. 2007
    ..The data point to a significant role for W16 in the gating of MscS, and an essential role for W240 in MscS oligomer stability...
  68. pmc A role for mechanosensitive channels in survival of stationary phase: regulation of channel expression by RpoS
    Neil R Stokes
    Department of Molecular and Cell Biology, Institute of Medical Sciences, University of Aberdeen, Aberdeen AB25 2ZD, Scotland, United Kingdom
    Proc Natl Acad Sci U S A 100:15959-64. 2003
    ..These data suggest that MscS and MscL are components of the RpoS regulon and play an important role in ensuring structural integrity in stationary phase bacteria...
  69. ncbi The structures of BtuCD and MscS and their implications for transporter and channel function
    Randal B Bass
    Division of Chemistry and Chemical Engineering, 114 96, California Institute of Technology, Pasadena, CA 91125, USA
    FEBS Lett 555:111-5. 2003
    ..aspects of the structure and mechanism of two bacterial transport systems, the stretch-activated mechanosensitive channel of small conductance (MscS) and the ATP-dependent vitamin B12 uptake system (BtuCD), emphasizing their ..
  70. pmc Pore mutations of the Escherichia coli MscS channel affect desensitization but not ionic preference
    Michelle D Edwards
    School of Medical Sciences, University of Aberdeen, Institute of Medical Sciences, Foresterhill, Aberdeen, AB25 2ZD, UK
    Biophys J 94:3003-13. 2008
    ..Mutations at residues further into the pore also regulated desensitization. Transition to this unique mechanosensitive channel state is discussed in terms of existing data.
  71. ncbi Mechanosensitive channels: stress relief
    Philip C Biggin
    Laboratory of Molecular Biophysics, Department of Biochemistry, The University of Oxford, South Parks Road, Oxford, OX1 3QU, UK
    Curr Biol 13:R183-5. 2003
    ..The crystal structure has been determined of the small conductance mechanosensitive channel (MscS) from Escherichia coli, providing new insights into mechanical and voltage sensing by this and ..
  72. ncbi Cloning and characterization of Edwardsiella ictaluri proteins expressed and recognized by the channel catfish Ictalurus punctatus immune response during infection
    Michelle M Moore
    Department of Veterinary Science, Louisiana State University Agricultural Center, School of Veterinary Medicine, Louisiana State University, Baton Rouge, Louisiana 70803, USA
    Dis Aquat Organ 52:93-107. 2002
    ..and 5d3 encoded ORFs similar to Escherichia coli partial genes serA and pgk, and complete genes rpiA, iciA, yggE, yggB and fda...
  73. ncbi Narrative medicine and unresolved, end-of-life longing
    Therese Schroeder-Sheker
    Explore (NY) 2:169-71. 2006
  74. ncbi Effect of high hydrostatic pressure on the bacterial mechanosensitive channel MscS
    A G Macdonald
    Department of Biomedical Sciences, University of Aberdeen, Aberdeen, AB25 2ZD, UK
    Eur Biophys J 34:434-41. 2005
    We have investigated the effect of high hydrostatic pressure on MscS, the bacterial mechanosensitive channel of small conductance. Pressure affected channel kinetics but not conductance...
  75. pmc Domain organization of the MscS mechanosensitive channel of Escherichia coli
    Samantha Miller
    Department of Molecular and Cell Biology, University of Aberdeen, Institute of Medical Sciences, Aberdeen AB25 2ZD, UK
    EMBO J 22:36-46. 2003
    The major structural features of the Escherichia coli MscS mechanosensitive channel protein have been explored using alkaline phosphatase (PhoA) fusions, precise deletions and site-directed mutations...