luxS

Summary

Gene Symbol: luxS
Description: S-ribosylhomocysteine lyase
Alias: ECK2681, JW2662, ygaG
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. ncbi Making 'sense' of metabolism: autoinducer-2, LuxS and pathogenic bacteria
    Agnès Vendeville
    Centre for Molecular Microbiology and Infection, Department of Infectious Diseases, Faculty of Medicine, Flowers Building, Armstrong Road, Imperial College London, London SW7 2AZ, UK
    Nat Rev Microbiol 3:383-96. 2005
  2. pmc Bacteria-host communication: the language of hormones
    Vanessa Sperandio
    Department of Microbiology, University of Texas Southwestern Medical Center, Dallas, TX 75390 9048, USA
    Proc Natl Acad Sci U S A 100:8951-6. 2003
  3. ncbi LuxS and autoinducer-2: their contribution to quorum sensing and metabolism in bacteria
    Klaus Winzer
    Institute of Infection, Immunity and Inflammation, Queen s Medical Centre, C Floor, West Block, Nottingham, NG7 2UH, U K
    Adv Appl Microbiol 53:291-396. 2003
  4. pmc Quorum sensing in Escherichia coli and Salmonella typhimurium
    M G Surette
    Department of Microbiology and Infectious Diseases, University of Calgary, 3330 Hospital Drive, North West, Calgary, Alberta, T2N 4N1, Canada
    Proc Natl Acad Sci U S A 95:7046-50. 1998
  5. pmc Quorum sensing in Escherichia coli, Salmonella typhimurium, and Vibrio harveyi: a new family of genes responsible for autoinducer production
    M G Surette
    Department of Microbiology and Infectious Diseases, University of Calgary, 3330 Hospital Drive, North West, Calgary, Alberta, T2N 4N1, Canada
    Proc Natl Acad Sci U S A 96:1639-44. 1999
  6. ncbi The LuxS family of bacterial autoinducers: biosynthesis of a novel quorum-sensing signal molecule
    S Schauder
    Department of Molecular Biology, Princeton University, Princeton, NJ 08544 1014, USA
    Mol Microbiol 41:463-76. 2001
  7. pmc Cyclic AMP (cAMP) and cAMP receptor protein influence both synthesis and uptake of extracellular autoinducer 2 in Escherichia coli
    Liang Wang
    Center for Biosystems Research, University of Maryland Biotechnology Institute, College Park, MD 20742, USA
    J Bacteriol 187:2066-76. 2005
  8. pmc luxS-dependent gene regulation in Escherichia coli K-12 revealed by genomic expression profiling
    Liang Wang
    Center for Biosystems Research, University of Maryland Biotechnology Institute, College Park, MD 20742, USA
    J Bacteriol 187:8350-60. 2005
  9. pmc Bacterial cell-to-cell signaling in the gastrointestinal tract
    James B Kaper
    Center for Vaccine Development, University of Maryland School of Medicine, 685 W Baltimore St, Baltimore, MD 21201, USA
    Infect Immun 73:3197-209. 2005
  10. ncbi LuxS: its role in central metabolism and the in vitro synthesis of 4-hydroxy-5-methyl-3(2H)-furanone
    Klaus Winzer
    Institute of Infections and Immunity, University of Nottingham, Queen s Medical Centre, Nottingham NG7 2UH, UK
    Microbiology 148:909-22. 2002

Research Grants

Scientific Experts

  • Kim Rachael Hardie
  • JAMES KAPER
  • Vanessa Sperandio
  • BRIAN AHMER
  • Klaus Winzer
  • Ivan Rychlik
  • Timothy J Tavender
  • Shawn R Campagna
  • R D Heal
  • Jorge A Giron
  • Eugene Kolker
  • Xiangan Han
  • Liang Wang
  • Matthew Walters
  • Yang Yang
  • Jun Li
  • Senthilkumar Palaniyandi
  • James J Valdes
  • William E Bentley
  • Richard Haigh
  • Kezong Qi
  • Shengqing Yu
  • Chan Ding
  • Hao Bai
  • Lei Liu
  • M G Surette
  • M P Delisa
  • Mariana Grillo-Puertas
  • Hua Ling
  • B L Bassler
  • Nicholas De Lay
  • A S Belik
  • Yevgeniy Turovskiy
  • Kamlesh Soni
  • Shirley Yang
  • Sigrid C J De Keersmaecker
  • K E Eboigbodin
  • Jinge Zhu
  • Yoshifumi Hashimoto
  • Chen Yu Tsao
  • Karina B Xavier
  • Agnès Vendeville
  • Thomas K Wood
  • Dacheng Ren
  • S K Anand
  • Philip R Hardwidge
  • Mingxu Zhou
  • Xinjun Zhang
  • Fenghua Yao
  • Xiaofang Zhu
  • Jun Zhu
  • Guoqiang Zhu
  • Huayan Hou
  • Hongliang Dong
  • Haiwen Liu
  • Arindam Mitra
  • Rui Liu
  • Primrose Freestone
  • Jun Song
  • Christopher D Herren
  • Suman Mukhopadhyay
  • Brijesh Kumar
  • Xiaoping Zhu
  • Sara Sandrini
  • Viviana A Rapisarda
  • W E Bentley
  • Qinghai Hu
  • Wenjing Chen
  • S Schauder
  • C Kirkpatrick
  • J J Valdes
  • M E Taga
  • Maria R Rintoul
  • Josefina M Villegas
  • Xiaobao Qi
  • Matthew Wook Chang
  • Mui Hua Tan
  • Aram Kang
  • Susan Gottesman
  • G B Zavil'gel'skiĭ
  • I A Khmel'
  • Michael L Chikindas
  • Brad Williams
  • Dimitri Kashtanov
  • Suresh D Pillai
  • Michael Hume
  • Boris Paskhover
  • Martha Cepeda
  • Arul Jayaraman
  • William K Russell

Detail Information

Publications51

  1. ncbi Making 'sense' of metabolism: autoinducer-2, LuxS and pathogenic bacteria
    Agnès Vendeville
    Centre for Molecular Microbiology and Infection, Department of Infectious Diseases, Faculty of Medicine, Flowers Building, Armstrong Road, Imperial College London, London SW7 2AZ, UK
    Nat Rev Microbiol 3:383-96. 2005
    ..One quorum-sensing system, based on the possession of LuxS, bears the unique feature of contributing directly to metabolism, and therefore has the potential to influence both ..
  2. pmc Bacteria-host communication: the language of hormones
    Vanessa Sperandio
    Department of Microbiology, University of Texas Southwestern Medical Center, Dallas, TX 75390 9048, USA
    Proc Natl Acad Sci U S A 100:8951-6. 2003
    ..The QS system used by EHEC is the LuxS/autoinducer 2 (AI-2) system extensively involved in interspecies communication...
  3. ncbi LuxS and autoinducer-2: their contribution to quorum sensing and metabolism in bacteria
    Klaus Winzer
    Institute of Infection, Immunity and Inflammation, Queen s Medical Centre, C Floor, West Block, Nottingham, NG7 2UH, U K
    Adv Appl Microbiol 53:291-396. 2003
  4. pmc Quorum sensing in Escherichia coli and Salmonella typhimurium
    M G Surette
    Department of Microbiology and Infectious Diseases, University of Calgary, 3330 Hospital Drive, North West, Calgary, Alberta, T2N 4N1, Canada
    Proc Natl Acad Sci U S A 95:7046-50. 1998
    ..Implications for the involvement of quorum sensing in pathogenesis are discussed...
  5. pmc Quorum sensing in Escherichia coli, Salmonella typhimurium, and Vibrio harveyi: a new family of genes responsible for autoinducer production
    M G Surette
    Department of Microbiology and Infectious Diseases, University of Calgary, 3330 Hospital Drive, North West, Calgary, Alberta, T2N 4N1, Canada
    Proc Natl Acad Sci U S A 96:1639-44. 1999
    ..E. coli DH5alpha can be complemented to AI-2 production by the introduction of the luxS gene from V. harveyi or E. coli O157:H7. Analysis of the E. coli DH5alpha luxSE.c...
  6. ncbi The LuxS family of bacterial autoinducers: biosynthesis of a novel quorum-sensing signal molecule
    S Schauder
    Department of Molecular Biology, Princeton University, Princeton, NJ 08544 1014, USA
    Mol Microbiol 41:463-76. 2001
    ..bacteria produce AI-2 and, in every case, production of AI-2 is dependent on the function encoded by the luxS gene...
  7. pmc Cyclic AMP (cAMP) and cAMP receptor protein influence both synthesis and uptake of extracellular autoinducer 2 in Escherichia coli
    Liang Wang
    Center for Biosystems Research, University of Maryland Biotechnology Institute, College Park, MD 20742, USA
    J Bacteriol 187:2066-76. 2005
    ..repression through the cyclic AMP (cAMP)-CRP complex, which directly stimulates transcription of the lsr (for "luxS regulated") operon and indirectly represses luxS expression...
  8. pmc luxS-dependent gene regulation in Escherichia coli K-12 revealed by genomic expression profiling
    Liang Wang
    Center for Biosystems Research, University of Maryland Biotechnology Institute, College Park, MD 20742, USA
    J Bacteriol 187:8350-60. 2005
    The bacterial quorum-sensing autoinducer 2 (AI-2) has received intense interest because the gene for its synthase, luxS, is common among a large number of bacterial species...
  9. pmc Bacterial cell-to-cell signaling in the gastrointestinal tract
    James B Kaper
    Center for Vaccine Development, University of Maryland School of Medicine, 685 W Baltimore St, Baltimore, MD 21201, USA
    Infect Immun 73:3197-209. 2005
  10. ncbi LuxS: its role in central metabolism and the in vitro synthesis of 4-hydroxy-5-methyl-3(2H)-furanone
    Klaus Winzer
    Institute of Infections and Immunity, University of Nottingham, Queen s Medical Centre, Nottingham NG7 2UH, UK
    Microbiology 148:909-22. 2002
    ..a number of bacteria have been shown to possess AI-2 activity in their culture supernatants, and bear the luxS gene product, which is required for AI-2 synthesis...
  11. pmc Mapping stress-induced changes in autoinducer AI-2 production in chemostat-cultivated Escherichia coli K-12
    M P Delisa
    Center for Agricultural Biotechnology, University of Maryland Biotechnology Institute, University of Maryland, College Park, Maryland 20742, USA
    J Bacteriol 183:2918-28. 2001
    ..Significant regulatory overlap was revealed among several stress and starvation genes and known quorum-sensing genes...
  12. pmc A stochastic model of Escherichia coli AI-2 quorum signal circuit reveals alternative synthesis pathways
    Jun Li
    Center for Biosystems Research, University of Maryland Biotechnology Institute, College Park, Maryland, MD, USA
    Mol Syst Biol 2:67. 2006
    ..Many cell functions are regulated by intricate and multimodal QS signal transduction processes. The LuxS/AI-2 QS system is highly conserved among Eubacteria and AI-2 is reported as a 'universal' signal molecule...
  13. ncbi Let LuxS speak up in AI-2 signaling
    Sigrid C J De Keersmaecker
    Centre of Microbial and Plant Genetics, K U Leuven, Kasteelpark Arenberg 20, 3001 Leuven, Belgium
    Trends Microbiol 14:114-9. 2006
    ..The AI-2 synthase LuxS is widely distributed among the Bacteria, which suggests that AI-2 is a language for interspecies communication...
  14. ncbi Differential gene expression shows natural brominated furanones interfere with the autoinducer-2 bacterial signaling system of Escherichia coli
    Dacheng Ren
    Department of Chemical Engineering and Molecular and Cell Biology, University of Connecticut, 191 Auditorium Road, U 3222, Storrs, Connecticut 06269 3222, USA
    Biotechnol Bioeng 88:630-42. 2004
    ..Microbiol 3:731-736, 2001) to inhibit quorum sensing in Escherichia coli via autoinducer-2 (AI-2, produced by LuxS)...
  15. pmc Interference with AI-2-mediated bacterial cell-cell communication
    Karina B Xavier
    Department of Molecular Biology, Howard Hughes Medical Institute, Princeton University, Princeton, New Jersey 08544 1014, USA
    Nature 437:750-3. 2005
    ..AI-2 signalling, and the interference with it, could have important ramifications for eukaryotes in the maintenance of normal microflora and in protection from pathogenic bacteria...
  16. doi Quorum-sensing gene luxS regulates flagella expression and Shiga-like toxin production in F18ab Escherichia coli
    Yang Yang
    a College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, People s Republic of China
    Can J Microbiol 60:355-61. 2014
    To investigate the effect of the luxS gene on the expression of virulence factors in Shiga-like toxin producing and verotoxin-producing Escherichia coli, the luxS gene from E. coli 107/86 (wild type, O139:H1:F18ab, Stx2e) was deleted...
  17. ncbi Proteomic analysis to identify the role of LuxS/AI-2 mediated protein expression in Escherichia coli O157:H7
    Kamlesh Soni
    Food Safety and Environmental Microbiology Program, Department of Poultry Science, Texas A and M University, College Station, Texas, 77843, USA
    Foodborne Pathog Dis 4:463-71. 2007
    ..coli O157:H7 and its isogenic luxS mutant, and 2) the luxS mutant and the luxS mutant supplemented with AI-2 molecules...
  18. pmc LuxS-independent formation of AI-2 from ribulose-5-phosphate
    Timothy J Tavender
    Institute of Infections, Immunity, and Inflammation, University of Nottingham, Centre for Biomolecular Sciences, Nottingham, NG7 2RD, UK
    BMC Microbiol 8:98. 2008
    ..signal molecule AI-2 is generated from its precursor S-ribosyl-L-homocysteine in a reaction catalysed by the enzyme LuxS. However, generation of AI-2-like activity has also been reported for organisms lacking the luxS gene and the ..
  19. doi LuxS contributes to virulence in avian pathogenic Escherichia coli O78:K80:H9
    Senthilkumar Palaniyandi
    Virginia Maryland Regional College of Veterinary Medicine, College Park, MD 20742, USA
    Vet Microbiol 166:567-75. 2013
    ..b>LuxS, a well-known controller in the QS, plays a role in regulating virulence in various bacterial species...
  20. ncbi Autoinducer 2 activity in Escherichia coli culture supernatants can be actively reduced despite maintenance of an active synthase, LuxS
    Kim R Hardie
    Institute of Infections and Immunity, Queen s Medical Centre, C Floor, West Block, Nottingham NG7 2UH, UK
    Microbiology 149:715-28. 2003
    Production of the signalling molecule (autoinducer-2) synthesized by LuxS has been proposed to be pivotal to a universal mechanism of inter-species bacterial cell-cell communication (quorum sensing); however recently the function of LuxS ..
  21. ncbi The LuxS-dependent autoinducer AI-2 controls the expression of an ABC transporter that functions in AI-2 uptake in Salmonella typhimurium
    M E Taga
    Department of Molecular Biology, Princeton University, Princeton, NJ 08544 1014, USA
    Mol Microbiol 42:777-93. 2001
    ..In every case, production of AI-2 is dependent on the LuxS autoinducer synthase...
  22. doi Mutation design and strain background influence the phenotype of Escherichia coli luxS mutants
    Richard Haigh
    Department of Genetics, University of Leicester, Leicester, UK
    Mol Microbiol 88:951-69. 2013
    Previous analyses of luxS in Escherichia coli have used different strain backgrounds and design formats to produce the luxS mutation, resulting in luxS mutants with confusingly dissimilar phenotypes...
  23. ncbi [Cloning and expression of luxS and pfs and in vitro biosynthesis autoinducer 2 of avian pathogenic Escherichia coli from Anhui Province]
    Xiangan Han
    Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences CAAS, Shanghai 200241, China
    Wei Sheng Wu Xue Bao 52:1167-72. 2012
    The LuxS/AI-2 quorum sensing (QS) system shared by Gram-positive and Gram-negative bacteria involves the production of autoinducer-2 (AI-2)...
  24. pmc Polyphosphate degradation in stationary phase triggers biofilm formation via LuxS quorum sensing system in Escherichia coli
    Mariana Grillo-Puertas
    Instituto Superior de Investigaciones Biológicas Consejo Nacional de Investigaciones Científicas y Técnicas Universidad Nacional de Tucumán, and Instituto de Química Biológica Dr Bernabé Bloj Universidad Nacional de Tucumán, San Miguel de Tucuman, Tucuman, Argentina
    PLoS ONE 7:e50368. 2012
    ..b>luxS and lsrK mutants, impaired in autoinducer-2 quorum sensing signal metabolism, were unable to form biofilm unless ..
  25. doi The luxS gene functions in the pathogenesis of avian pathogenic Escherichia coli
    Xiangan Han
    Shanghai Veterinary Research Institute, The Chinese Academy of Agricultural Sciences, Shanghai, PR China
    Microb Pathog 55:21-7. 2013
    ..b>LuxS, the product of the luxS gene, mediates the quorum sensing (QS) mechanism...
  26. pmc AI-3 synthesis is not dependent on luxS in Escherichia coli
    Matthew Walters
    University of Texas Southwestern Medical Center, Department of Microbiology, 5323 Harry Hines Blvd, Dallas, TX 75390 9048, USA
    J Bacteriol 188:5668-81. 2006
    ..with homocysteine, is produced by cleavage of S-adenosylhomocysteine (SAH) and S-ribosylhomocysteine by the Pfs and LuxS enzymes. Numerous phenotypes have been attributed to AI-2 QS signaling using luxS mutants...
  27. pmc Emr, an Escherichia coli locus for multidrug resistance
    O Lomovskaya
    Department of Biology, Massachusetts Institute of Technology, Cambridge 02139
    Proc Natl Acad Sci U S A 89:8938-42. 1992
    ..Disruption of emrB significantly increased sensitivity of cells to uncouplers. The cellular content of uncoupler increased in the order: overexpressed emrB cells greater than wild type greater than emrB-...
  28. doi The absence of the luxS gene increases swimming motility and flagella synthesis in Escherichia coli K12
    Hua Ling
    School of Chemical and Biomedical Engineering, Nanyang Technological University, Singapore 637459, Singapore
    Biochem Biophys Res Commun 401:521-6. 2010
    Despite the significant role of S-ribosylhomocysteinase (LuxS) in the activated methyl cycle pathway and quorum sensing, the connectivity between luxS and other cellular functions remains incomplete...
  29. doi Direct quantitation of the quorum sensing signal, autoinducer-2, in clinically relevant samples by liquid chromatography-tandem mass spectrometry
    Shawn R Campagna
    Department of Chemistry, The University of Tennessee, Knoxville, Tennessee 37996, USA
    Anal Chem 81:6374-81. 2009
    ....
  30. pmc The Crp-activated small noncoding regulatory RNA CyaR (RyeE) links nutritional status to group behavior
    Nicholas De Lay
    Laboratory of Molecular Biology, National Cancer Institute, Bethesda, Maryland 20892 4264, USA
    J Bacteriol 191:461-76. 2009
    ..that several genes are negatively regulated by CyaR, including ompX, encoding a major outer membrane protein; luxS, encoding the autoinducer-2 synthase; nadE, encoding an essential NAD synthetase; and yqaE, encoding a predicted ..
  31. ncbi [Influence of mutations at genes of global transcriptional regulators on production of autoinducer AI-2 Quorum Sensing in the system of Escherichia coli]
    A S Belik
    Genetika 44:1184-90. 2008
    ..AI-2 and synthase LuxS mediating its synthesis are widely distributed in Gram-negative and Gram-positive bacteria...
  32. pmc Quorum sensing: fact, fiction, and everything in between
    Yevgeniy Turovskiy
    Department of Food Science, Rutgers, The State University of New Jersey, New Brunswick, New Jersey 08901, USA
    Adv Appl Microbiol 62:191-234. 2007
  33. ncbi Catalytic mechanism of S-ribosylhomocysteinase: ionization state of active-site residues
    Jinge Zhu
    Department of Chemistry and Ohio State Biochemistry Program, The Ohio State University, 100 West 18th Avenue, Columbus, Ohio 43210, USA
    Biochemistry 45:12195-203. 2006
    S-Ribosylhomocysteinase (LuxS) catalyzes the cleavage of the thioether linkage in S-ribosylhomocysteine (SRH) to produce homocysteine (Hcys) and 4,5-dihydroxy-2,3-pentanedione (DPD), the precursor of type II bacterial autoinducer (AI-2)...
  34. ncbi Bacterial quorum sensing and cell surface electrokinetic properties
    K E Eboigbodin
    Department of Chemical and Process Engineering, The University of Sheffield, Mappin Street, Sheffield S1 3JD, UK
    Appl Microbiol Biotechnol 73:669-75. 2006
    ..Escherichia coli MG1655 and MG1655 LuxS- mutant (lacking quorum-sensing gene for Autoinducer synthase AI-2) were used for this study...
  35. ncbi Quorum sensing in Escherichia coli and Salmonella
    Matthew Walters
    Department of Microbiology, University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd, Dallas, TX 75390 9048, USA
    Int J Med Microbiol 296:125-31. 2006
    ..several research groups have demonstrated that these bacteria use several quorum-sensing systems, such as: the luxS/AI-2, AI-3/epinephrine/norepinephrine, indole, and the LuxR homolog SdiA to achieve intercellular signaling...
  36. ncbi Escherichia coli gets the message
    Klaus Winzer
    Nat Med 9:1118-9. 2003
  37. pmc Quorum sensing and multidrug transporters in Escherichia coli
    Shirley Yang
    Department of Molecular Virology and Microbiology and Interdepartmental Program in Cell and Molecular Biology, Baylor College of Medicine, 1 Baylor Plaza, Houston, TX 77030, USA
    Proc Natl Acad Sci U S A 103:2386-91. 2006
    ..These results were not affected by the deletion of luxS, which synthesizes the quorum-sensing signal autoinducer 2 (AI-2)...
  38. ncbi Cell-to-cell signalling in Escherichia coli and Salmonella enterica
    Brian M M Ahmer
    Department of Microbiology, The Ohio State University, 484 West 12th Avenue, 376 Biological Sciences Building, Columbus, OH 43210, USA
    Mol Microbiol 52:933-45. 2004
    ..Conversely, E. coli and Salmonella encode the signal-generating component of a second system (a LuxS homologue that generates AI-2), but the sensory apparatus for AI-2 differs substantially from the Vibrio system...
  39. ncbi Bacterial cell-to-cell communication: sorry, can't talk now - gone to lunch!
    Klaus Winzer
    Institute of Infections and Immunity, University of Nottingham, Queen s Medical Centre, NG7 2UH, Nottingham, United Kingdom
    Curr Opin Microbiol 5:216-22. 2002
    ..cell-to-cell communication (quorum sensing) systems based on diffusible signal molecules, such as indole and the LuxS autoinducer-2, requires discrimination between true signalling molecules and metabolites present in culture ..
  40. pmc Acetate and formate stress: opposite responses in the proteome of Escherichia coli
    C Kirkpatrick
    Department of Biology, Kenyon College, Gambier, Ohio 43022, USA
    J Bacteriol 183:6466-77. 2001
    ..and ProX), metabolic enzymes (YfiD and GatY), the RpoS growth phase regulon, and the autoinducer synthesis protein LuxS. Acetate repressed 17 proteins, among them phosphotransferase (Pta)...
  41. ncbi Salmonella stress management and its relevance to behaviour during intestinal colonisation and infection
    Ivan Rychlik
    Veterinary Research Institute, Hudcova 70, 621 32 Brno, Czech Republic
    FEMS Microbiol Rev 29:1021-40. 2005
    ..The role of quorum-sensing through luxS and sdiA is also considered as a regulator of virulence and colonisation.
  42. ncbi Quorum sensing and expression of virulence in Escherichia coli O157:H7
    S K Anand
    National Dairy Research Institute, 132001, Karnal, India
    Int J Food Microbiol 85:1-9. 2003
    ..coli and more specifically O157:H7, have been shown to produce AI-2 depending on the function encoded by the luxS gene. The LuxS acts as an AI-2 synthase and the AI-2 is produced from S-adenosylmethionine in three enzymatic steps...
  43. ncbi Novel intercellular communication system in Escherichia coli that confers antibiotic resistance between physically separated populations
    R D Heal
    Biophysics Research Group, QinetiQ Ltd, Winfrith Technology Centre, Dorchester, UK
    J Appl Microbiol 92:1116-22. 2002
    ..To determine whether intercellular signalling can occur between physically separated populations of Escherichia coli...
  44. ncbi The flagella of enteropathogenic Escherichia coli mediate adherence to epithelial cells
    Jorge A Giron
    Centro de Investigaciones en Ciencias Microbiológicas, Instituto de Ciencias, Benemerita Universidad Autonoma de Puebla, Mexico
    Mol Microbiol 44:361-79. 2002
    ..Isogenic strains mutated in perA (a transcriptional activator), bfpA (a type IV pilin), luxS (a quorum-sensing autoinducer gene) and in the type III secretion genes were reduced for motility in Dulbecco's ..
  45. pmc Quorum sensing is a global regulatory mechanism in enterohemorrhagic Escherichia coli O157:H7
    V Sperandio
    Center for Vaccine Development and Department of Microbiology and Immunology, University of Maryland School of Medicine, Baltimore, Maryland 21201, USA
    J Bacteriol 183:5187-97. 2001
    ..of the AE lesion were regulated by quorum sensing through autoinducer-2, which is synthesized by the product of the luxS gene. In this study we hybridized an E...
  46. ncbi Construction of a contiguous 874-kb sequence of the Escherichia coli -K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features
    Y Yamamoto
    Department of Genetics, Hyogo College of Medicine, Nishinomiya, Japan
    DNA Res 4:91-113. 1997
    ..An isoleucine tRNA gene, designated ileY, was also newly identified at 60.0 min...
  47. pmc Quorum sensing controls expression of the type III secretion gene transcription and protein secretion in enterohemorrhagic and enteropathogenic Escherichia coli
    V Sperandio
    Center for Vaccine Development, Department of Microbiology, University of Maryland School of Medicine, 685 West Baltimore Street, Baltimore, MD 21201, USA
    Proc Natl Acad Sci U S A 96:15196-201. 1999
    ..coli and enteropathogenic E. coli. The luxS gene recently shown to be responsible for production of autoinducer in the Vibrio harveyi and E...
  48. pmc DNA microarray-based identification of genes controlled by autoinducer 2-stimulated quorum sensing in Escherichia coli
    M P Delisa
    Center for Agricultural Biotechnology, University of Maryland Biotechnology Institute, University of Maryland, College Park, Maryland 20742, USA
    J Bacteriol 183:5239-47. 2001
    ..We investigated global changes in mRNA abundance elicited by the AI-2 signaling molecule through the use of a luxS mutant that was unable to synthesize AI-2. Remarkably, 242 genes, comprising ca. 5.6% of the E...
  49. pmc Detection of new genes in a bacterial genome using Markov models for three gene classes
    M Borodovsky
    School of Biology, Georgia Institute of Technology, Atlanta 30332, USA
    Nucleic Acids Res 23:3554-62. 1995
    ....
  50. pmc Identification and functional analysis of 'hypothetical' genes expressed in Haemophilus influenzae
    Eugene Kolker
    BIATECH, 19310 North Creek Parkway, Suite 115, Bothell, WA 98011, USA
    Nucleic Acids Res 32:2353-61. 2004
    ....

Research Grants7

  1. MOLECULAR GENETICS OF ENTEROPATHOGENIC E COLI ADHESION
    JAMES KAPER; Fiscal Year: 2003
    ..The proposed experimental approach will use a combination of molecular genetics, cell biology, and animal studies to achieve a better understanding of how EPEC infects intestinal epithelial cells and causes disease. ..
  2. EHEC interactions with the normal intestinal flora
    Vanessa Sperandio; Fiscal Year: 2004
    ..In Specific Aim 2, we propose to monitor EHEC virulence gene expression in a mixed population in the presence of the intestinal flora. ..
  3. Detection of Mixed Microbial Communities by Salmonella
    BRIAN AHMER; Fiscal Year: 2004
    ..We will use microarrays to identify the portions of the E. coli and S. typhimurium genomes that are regulated by SdiA in response to AHLs and characterize the phenotypic consequences of this signaling event. ..
  4. Screen for Quorum Sensing Molecular Inhibitors (RMI)
    Vanessa Sperandio; Fiscal Year: 2005
    ....
  5. NOVEL E. COLI 0157:H7 INTESTINAL COLONIZATION FACTORS
    JAMES KAPER; Fiscal Year: 2009
    ....
  6. DEVELOPMENT OF A LIVE ORAL CHOLERA VACCINE
    James B Kaper; Fiscal Year: 2010
    ..Lay summary: Vibrio cholerae is a bacterium that causes cholera, a severe diarrheal disease that can often cause death. This project will develop improved vaccines for the prevention of cholera. ..