hmp

Summary

Gene Symbol: hmp
Description: fused nitric oxide dioxygenase/dihydropteridine reductase 2
Alias: ECK2549, JW2536, fsrB, hmpA
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. pmc Nitric oxide dioxygenase: an enzymic function for flavohemoglobin
    P R Gardner
    Division of Critical Care Medicine, Children s Hospital Medical Center, and Department of Pediatrics, University of Cincinnati, 3333 Burnet Avenue, Cincinnati, OH 45229, USA
    Proc Natl Acad Sci U S A 95:10378-83. 1998
  2. ncbi Nitric oxide and nitrosative stress tolerance in bacteria
    R K Poole
    Department of Molecular Biology and Biotechnology, The University of Sheffield, Firth Court, Western Bank, Sheffield S10 2TN, UK
    Biochem Soc Trans 33:176-80. 2005
  3. pmc Nitric oxide, nitrite, and Fnr regulation of hmp (flavohemoglobin) gene expression in Escherichia coli K-12
    R K Poole
    Division of Life Sciences, King s College London, United Kingdom
    J Bacteriol 178:5487-92. 1996
  4. doi The influence of CsgD on the expression of genes of folate metabolism and hmp in Escherichia coli K-12
    Muriel B Herrington
    Biology Department, Concordia University, 7141 Sherbrooke St W, Montreal, QC H4B 1R6, Canada
    Arch Microbiol 195:559-69. 2013
  5. ncbi Flavohemoglobin Hmp, but not its individual domains, confers protection from respiratory inhibition by nitric oxide in Escherichia coli
    Elizabeth Hernández-Urzúa
    Laboratorio de Microbiología y Genética Molecular, Departamento de Biologia Molecular y Biotecnologia, Instituto de Investigaciones Biomedicas, P O Box 70 228, Mexico City, 04510, Mexico
    J Biol Chem 278:34975-82. 2003
  6. ncbi Flavohemoglobin, a globin with a peroxidase-like catalytic site
    M Mukai
    Department of Physiology and Biophysics, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    J Biol Chem 276:7272-7. 2001
  7. pmc Escherichia coli flavohaemoglobin (Hmp) with equistoichiometric FAD and haem contents has a low affinity for dioxygen in the absence or presence of nitric oxide
    C E Mills
    Krebs Institute for Biomolecular Research, Department of Molecular Biology and Biotechnology, The University of Sheffield, Firth Court, Sheffield, S10 2TN, U K
    Biochem J 353:207-13. 2001
  8. pmc Flavohemoglobin denitrosylase catalyzes the reaction of a nitroxyl equivalent with molecular oxygen
    A Hausladen
    Department of Medicine, Duke University Medical Center, Durham, NC 27710, USA
    Proc Natl Acad Sci U S A 98:10108-12. 2001
  9. ncbi Novel hemoglobins to enhance microaerobic growth and substrate utilization in Escherichia coli
    C J Bollinger
    Institute of Biotechnology, ETH Zurich, CH 8093 Zurich, Switzerland
    Biotechnol Prog 17:798-808. 2001
  10. ncbi Flavohemoglobin detoxifies nitric oxide in aerobic, but not anaerobic, Escherichia coli. Evidence for a novel inducible anaerobic nitric oxide-scavenging activity
    Anne M Gardner
    Division of Critical Care Medicine, Children s Hospital Medical Center, Cincinnati, Ohio 45229, USA
    J Biol Chem 277:8166-71. 2002

Scientific Experts

  • R K Poole
  • Muriel B Herrington
  • Dmitry A Rodionov
  • Alessandra Bonamore
  • J Membrillo-Hernandez
  • Alberto Boffi
  • P R Gardner
  • Elizabeth Hernández-Urzúa
  • Hazel Corker
  • Andrea Ilari
  • Steven T Pullan
  • Anne M Gardner
  • M N Hughes
  • C E Mills
  • Lovisa Svensson
  • A Hausladen
  • A M Gardner
  • Ye Jin Eun
  • S O Kim
  • Tania M Stevanin
  • N Ioannidis
  • Diane M Bodenmiller
  • Jorge Membrillo-Hernández
  • Janet Flatley
  • T M Stevanin
  • Paola D'Angelo
  • Guanghui Wu
  • L A Martin
  • Alexander D Frey
  • M Eugenia Schininà
  • M F Anjum
  • Anna Farina
  • Paul R Gardner
  • C J Bollinger
  • A Bonamore
  • M Mukai
  • Katarina Persson
  • Sigge Strid
  • Thomas Schön
  • Nicola Gilberthorpe
  • Mirjana Poljakovic
  • Y Dou
  • J S Olson
  • Susanne Säve
  • J S Stamler
  • Nese Kurt
  • Silvia Cavagnero
  • Lucy Lee
  • Claire E Monk
  • Ashok Sekhar
  • Enrique Morett
  • Jose Ponce-Coria
  • David S Zamorano-Sánchez
  • M D Coopamah
  • Susan Grogan
  • Robert C Read
  • A L Salzman
  • Stephen Spiro
  • Martin N Hughes
  • Jason Barrett
  • Jeffrey Green
  • Alessandro Feis
  • Debora Lucarelli
  • Jean Louis Hazemann
  • Giulietta Smulevich
  • Maurizio Benfatto
  • Yutaka Orii
  • G M Cook
  • Stefano Della Longa
  • Edgardo Escamilla
  • Maurizio Gattoni
  • Pauli T Kallio
  • Y Orii
  • Catherine E Mills
  • Andrea Bellelli
  • Gregory P White
  • Patrizia Gentili
  • Subhash G Vasudevan
  • Martha L Contreras-Zentella
  • Ryan A Helmick
  • Kenneth A Johnson
  • S G Vasudevan
  • B Søballe
  • S Sedelnikova
  • S R Yeh
  • A Gow
  • J E Bailey
  • A Boffi
  • P T Kallio
  • E Chiancone

Detail Information

Publications53

  1. pmc Nitric oxide dioxygenase: an enzymic function for flavohemoglobin
    P R Gardner
    Division of Critical Care Medicine, Children s Hospital Medical Center, and Department of Pediatrics, University of Cincinnati, 3333 Burnet Avenue, Cincinnati, OH 45229, USA
    Proc Natl Acad Sci U S A 95:10378-83. 1998
    ....
  2. ncbi Nitric oxide and nitrosative stress tolerance in bacteria
    R K Poole
    Department of Molecular Biology and Biotechnology, The University of Sheffield, Firth Court, Western Bank, Sheffield S10 2TN, UK
    Biochem Soc Trans 33:176-80. 2005
    ..However, the most fully understood mechanism for NO detoxification involves the enterobacterial flavohaemoglobin (Hmp)...
  3. pmc Nitric oxide, nitrite, and Fnr regulation of hmp (flavohemoglobin) gene expression in Escherichia coli K-12
    R K Poole
    Division of Life Sciences, King s College London, United Kingdom
    J Bacteriol 178:5487-92. 1996
    Escherichia coli possesses a soluble flavohemoglobin, with an unknown function, encoded by the hmp gene...
  4. doi The influence of CsgD on the expression of genes of folate metabolism and hmp in Escherichia coli K-12
    Muriel B Herrington
    Biology Department, Concordia University, 7141 Sherbrooke St W, Montreal, QC H4B 1R6, Canada
    Arch Microbiol 195:559-69. 2013
    ..We compared the expression of genes involved in folate metabolism and a gene (hmp) in strains with an intact csgD gene and with a deletion in csgD...
  5. ncbi Flavohemoglobin Hmp, but not its individual domains, confers protection from respiratory inhibition by nitric oxide in Escherichia coli
    Elizabeth Hernández-Urzúa
    Laboratorio de Microbiología y Genética Molecular, Departamento de Biologia Molecular y Biotecnologia, Instituto de Investigaciones Biomedicas, P O Box 70 228, Mexico City, 04510, Mexico
    J Biol Chem 278:34975-82. 2003
    Escherichia coli possesses a two-domain flavohemoglobin, Hmp, implicated in nitric oxide (NO) detoxification...
  6. ncbi Flavohemoglobin, a globin with a peroxidase-like catalytic site
    M Mukai
    Department of Physiology and Biophysics, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    J Biol Chem 276:7272-7. 2001
    Biochemical studies of flavohemoglobin (Hmp) from Escherichia coli suggest that instead of aerobic oxygen delivery, a dioxygenase converts NO to NO3(-) and anaerobically, an NO reductase converts NO to N(2)O...
  7. pmc Escherichia coli flavohaemoglobin (Hmp) with equistoichiometric FAD and haem contents has a low affinity for dioxygen in the absence or presence of nitric oxide
    C E Mills
    Krebs Institute for Biomolecular Research, Department of Molecular Biology and Biotechnology, The University of Sheffield, Firth Court, Sheffield, S10 2TN, U K
    Biochem J 353:207-13. 2001
    A purification procedure for flavohaemoglobin Hmp (NO oxygenase) is described that gives high yields of protein with equistoichiometric haem and FAD contents...
  8. pmc Flavohemoglobin denitrosylase catalyzes the reaction of a nitroxyl equivalent with molecular oxygen
    A Hausladen
    Department of Medicine, Duke University Medical Center, Durham, NC 27710, USA
    Proc Natl Acad Sci U S A 98:10108-12. 2001
    We have previously reported that bacterial flavohemoglobin (HMP) catalyzes both a rapid reaction of heme-bound O(2) with nitric oxide (NO) to form nitrate [HMP-Fe(II)O(2) + NO --> HMP-Fe(III) + NO(3)(-)] and, under anaerobic ..
  9. ncbi Novel hemoglobins to enhance microaerobic growth and substrate utilization in Escherichia coli
    C J Bollinger
    Institute of Biotechnology, ETH Zurich, CH 8093 Zurich, Switzerland
    Biotechnol Prog 17:798-808. 2001
    ..eutropha FHP as a query sequence for genes (hmp) encoding hemoglobin-like proteins...
  10. ncbi Flavohemoglobin detoxifies nitric oxide in aerobic, but not anaerobic, Escherichia coli. Evidence for a novel inducible anaerobic nitric oxide-scavenging activity
    Anne M Gardner
    Division of Critical Care Medicine, Children s Hospital Medical Center, Cincinnati, Ohio 45229, USA
    J Biol Chem 277:8166-71. 2002
    ..flavoHb possesses nitrosoglutathione and nitrite reductase activities that may account for the protection it affords against these agents. NO detoxification by flavoHb occurs most effectively via O(2)-dependent NO dioxygenation...
  11. ncbi Flavorubredoxin, an inducible catalyst for nitric oxide reduction and detoxification in Escherichia coli
    Anne M Gardner
    Division of Critical Care Medicine, Children s Hospital Medical Center, Cincinnati, Ohio 45229, USA
    J Biol Chem 277:8172-7. 2002
    ..coli, respectively, whereas both enzymes scavenged NO under microaerobic conditions. We suggest designation of the ygaA-ygaK-ygbD gene cluster as the norRVW modulon for NO reduction and detoxification...
  12. ncbi The X-ray structure of ferric Escherichia coli flavohemoglobin reveals an unexpected geometry of the distal heme pocket
    Andrea Ilari
    CNR Center of Molecular Biology and the Department of Biochemical Sciences, University of La Sapienza, 00185 Rome, Italy
    J Biol Chem 277:23725-32. 2002
    ..Surprisingly, imidazole, cyanide, and azide binding profiles at equilibrium are not accounted for by a single site titration curve but are biphasic and strongly suggest the presence of two distinct conformers within the liganded species...
  13. ncbi Escherichia coli flavohemoglobin is an efficient alkylhydroperoxide reductase
    Alessandra Bonamore
    CNR Istituto di Biologia e Patologia Molecolare, Department of Biochemical Sciences, University La Sapienza, 00185 Rome, Italy
    J Biol Chem 278:22272-7. 2003
    Escherichia coli flavohemoglobin (HMP) is shown to be capable of catalyzing the reduction of several alkylhydroperoxide substrates into their corresponding alcohols using NADH as an electron donor...
  14. pmc Spectroscopic studies on an oxygen-binding haemoglobin-like flavohaemoprotein from Escherichia coli
    N Ioannidis
    Division of Biosphere Sciences, King s College, University of London, U K
    Biochem J 288:649-55. 1992
    The Escherichia coli haemoglobin-like flavohaemoprotein (Hmp) has been purified to near homogeneity using two chromatographic steps. The prosthetic groups are identified as FAD and protohaem IX...
  15. ncbi Nitric oxide formation by Escherichia coli. Dependence on nitrite reductase, the NO-sensing regulator Fnr, and flavohemoglobin Hmp
    Hazel Corker
    Department of Molecular Biology and Biotechnology, The University of Sheffield, Firth Court, Western Bank, Sheffield S10 2TN, United Kingdom
    J Biol Chem 278:31584-92. 2003
    ..bacterial NO-detoxifying enzymes, the best understood being the flavohemoglobins exemplified by Escherichia coli Hmp. However, many bacteria, including E...
  16. doi Flavohemoglobin: structure and reactivity
    Alessandra Bonamore
    Department of Biochemical Sciences, University of Roma La Sapienza, Piazzale Aldo Moro 5, Roma, Italy
    IUBMB Life 60:19-28. 2008
    ..The flavoHb from Escherichia coli (HMP) has been the object of a large number of investigations to unveil its physiological role in the framework of ..
  17. pmc Unusual heme iron-lipid acyl chain coordination in Escherichia coli flavohemoglobin
    Paola D'Angelo
    Department of Chemistry University La Sapienza, Rome, and Istituto Nazionale per la Fisica della Materia UdF, Camerino, Italy
    Biophys J 86:3882-92. 2004
    ..Thus, the contributions at 2.7 A distance from the heme iron are assigned to a couple of carbon atoms of the lipid acyl chain, possibly corresponding to the unsaturated carbons of the linoleic acid...
  18. doi Role of flavohemoglobin in combating nitrosative stress in uropathogenic Escherichia coli--implications for urinary tract infection
    Lovisa Svensson
    School of Natural Sciences, Linnaeus University, Kalmar, Sweden
    Microb Pathog 49:59-66. 2010
    ..This study investigates the significance of the NO-detoxifying enzyme flavohemoglobin (Hmp) in protection of uropathogenic Escherichia coli (UPEC) against nitrosative stress...
  19. ncbi Escherichia coli Hmp, an "oxygen-binding flavohaemoprotein", produces superoxide anion and self-destructs
    Guanghui Wu
    Department of Molecular Biology and Biotechnology, The University of Sheffield, Firth Court, Western Bank, Sheffield, S10 2TN, UK
    Arch Microbiol 182:193-203. 2004
    Escherichia coli Hmp is a homologue of Ralstonia eutropha FHP, the first reported bacterial flavohaemoglobin, and functions in NO detoxification...
  20. doi Microbial responses to nitric oxide and nitrosative stress: growth, "omic," and physiological methods
    Steven T Pullan
    Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield, United Kingdom
    Methods Enzymol 437:499-519. 2008
    ..A key to the literature describing methods suitable for assessing toxicity to microbes of NO and reactive nitrogen species is given...
  21. pmc Dissimilatory metabolism of nitrogen oxides in bacteria: comparative reconstruction of transcriptional networks
    Dmitry A Rodionov
    Institute for Information Transmission Problems, Russian Academy of Sciences, Moscow, Russia
    PLoS Comput Biol 1:e55. 2005
    ..The most conserved member of the predicted NsrR regulon is the NO-detoxifying flavohemoglobin Hmp. In enterobacteria, the regulon also includes two nitrite-responsive loci, nipAB (hcp-hcr) and nipC (dnrN), thus ..
  22. pmc The yjeB (nsrR) gene of Escherichia coli encodes a nitric oxide-sensitive transcriptional regulator
    Diane M Bodenmiller
    School of Biology, Georgia Institute of Technology, 310 Ferst Dr, Atlanta, GA 30332 0230, USA
    J Bacteriol 188:874-81. 2006
    ..We identify here the product of the yjeB gene as a negative regulator of the transcription of the ytfE, hmpA and ygbA genes, all of which are known to be upregulated by nitrosative stress...
  23. ncbi Multiple regulators of the Flavohaemoglobin (hmp) gene of Salmonella enterica serovar Typhimurium include RamA, a transcriptional regulator conferring the multidrug resistance phenotype
    Elizabeth Hernández-Urzúa
    Laboratorio de Microbiología y Genética Molecular, Departamento de Biologia Molecular y Biotecnologia, Instituto de Investigaciones Biomedicas, Universidad Nacional Autonoma de Mexico, PO Box 70 228, Coyoacan, Mexico City 04510, Mexico
    Arch Microbiol 187:67-77. 2007
    ..b>hmp mutant strains of several bacteria are hypersensitive to NO and related compounds and hmp genes are up-regulated by ..
  24. ncbi The hmp gene encoding the NO-inducible flavohaemoglobin in Escherichia coli confers a protective advantage in resisting killing within macrophages, but not in vitro: links with swarming motility
    Tania M Stevanin
    The University of Sheffield, Division of Genomic Medicine, Beech Hill Road, Sheffield, S10 2RX, UK
    Gene 398:62-8. 2007
    Escherichia coli flavohaemoglobin (Hmp) is the best-understood nitric oxide (NO) detoxifying protein and exhibits a robust dioxygenase activity, converting NO to nitrate ion with oxygen as co-substrate...
  25. doi Thermodynamic and kinetic characterization of apoHmpH, a fast-folding bacterial globin
    Ye Jin Eun
    Department of Biochemistry, University of Wisconsin Madison, 433 Babcock Drive, Madison, WI 53706, USA
    J Mol Biol 376:879-97. 2008
    ..This study supports the view that the general folding features of bacterial and eukaryotic globins are preserved through evolution while kinetic details differ...
  26. ncbi Haem, flavin and oxygen interactions in Hmp, a flavohaemoglobin from Escherichia coli
    C E Cooper
    Department of Paediatrics, University College London Medical School, Rayne Institute, U K
    Biochem Soc Trans 22:709-13. 1994
  27. ncbi Reactions of the Escherichia coli flavohaemoglobin (Hmp) with oxygen and reduced nicotinamide adenine dinucleotide: evidence for oxygen switching of flavin oxidoreduction and a mechanism for oxygen sensing
    R K Poole
    Division of Life Sciences, King s College London, U K
    Proc Biol Sci 255:251-8. 1994
    The soluble flavohaemoglobin (Hmp) of Escherichia coli contains haem B and FAD in a single 44 kDa polypeptide, and shows NADH oxidase activity...
  28. ncbi Ferric reductases in Escherichia coli: the contribution of the haemoglobin-like protein
    M Eschenbrenner
    Laboratoire d Etudes Dynamiques et Structurales de la Sélectivité, Universite Joseph Fourier, Grenoble, France
    Biochem Biophys Res Commun 198:127-31. 1994
    The haemoglobin-like protein (HMP) of E. coli previously isolated as a dihydropteridine reductase was shown to be also a ferric citrate reductase...
  29. pmc Accumulation of the F plasmid TraJ protein in cpx mutants of Escherichia coli
    P M Silverman
    Program in Molecular and Cell Biology, Oklahoma Medical Research Foundation, Oklahoma City 73104
    J Bacteriol 175:921-5. 1993
    ....
  30. ncbi The flavohaemoglobin (HMP) of Escherichia coli generates superoxide in vitro and causes oxidative stress in vivo
    J Membrillo-Hernandez
    Division of Life Sciences, King s College London, U K
    FEBS Lett 382:141-4. 1996
    Purified flavohaemoglobin (HMP) of Escherichia coli reduces Fe(III) in a superoxide dismutase (SOD)-sensitive reaction, demonstrating superoxide anion generation during aerobic NADH oxidation...
  31. ncbi Distribution of the flavohaemoglobin, HMP, between periplasm and cytoplasm in Escherichia coli
    S G Vasudevan
    Department of Molecular Sciences, James Cook University, Townsville, Australia
    FEMS Microbiol Lett 125:219-24. 1995
    The subcellular distribution of the soluble flavohaemoglobin (HMP) of Escherichia coli has been determined...
  32. ncbi Reactions of the Escherichia coli flavohaemoglobin (Hmp) with NADH and near-micromolar oxygen: oxygen affinity of NADH oxidase activity
    R K Poole
    Division of Life Sciences, King s College London, UK
    Microbiology 142:1141-8. 1996
    The soluble flavohaemoglobin (Hmp) of Escherichia coli, product of the hmp gene, contains haem B and FAD in a single polypeptide of molecular mass 44 kDa...
  33. pmc Paraquat regulation of hmp (flavohemoglobin) gene expression in Escherichia coli K-12 is SoxRS independent but modulated by sigma S
    J Membrillo-Hernandez
    Krebs Institute for Biomolecular Research, Department of Molecular Biology and Biotechnology, The University of Sheffield, United Kingdom
    J Bacteriol 179:3164-70. 1997
    We report the first example of a gene, hmp, encoding a soluble flavohemoglobin in Escherichia coli K-12, which is up-regulated by paraquat in a SoxRS-independent manner...
  34. ncbi Flavohemoglobin Hmp affords inducible protection for Escherichia coli respiration, catalyzed by cytochromes bo' or bd, from nitric oxide
    T M Stevanin
    Krebs Institute for Biomolecular Research, Department of Molecular Biology and Biotechnology, The University of Sheffield, Firth Court, Western Bank, Sheffield S10 2TN, United Kingdom
    J Biol Chem 275:35868-75. 2000
    ..Respiration of an hmp mutant defective in flavohemoglobin (Hmp) synthesis is extremely NO-sensitive (I(50) about 0...
  35. ncbi Escherichia coli flavohaemoglobin (Hmp) reduces cytochrome c and Fe(III)-hydroxamate K by electron transfer from NADH via FAD: sensitivity of oxidoreductase activity to haem-bound dioxygen
    R K Poole
    Division of Life Sciences, King s College London, UK
    Microbiology 143:1557-65. 1997
    Escherichia coli flavohaemoglobin (Hmp) reduced purified mitochondrial cytochrome c aerobically in a reaction that was not substantially inhibited by superoxide dismutase, demonstrating that superoxide anion, the product of O2 reduction ..
  36. ncbi Roles of RpoS (sigmaS), IHF and ppGpp in the expression of the hmp gene encoding the flavohemoglobin (Hmp) of Escherichia coli K-12
    J Membrillo-Hernandez
    The Krebs Institute for Biomolecular Research, Department of Molecular Biology and Biotechnology, The University of Sheffield, UK
    Mol Gen Genet 254:599-603. 1997
    The Escherichia coli K-12 gene hmp encodes the flavohemoglobin Hmp. A hmp promoter phi(hmp-lacZ)-operon fusion was constructed in the chromosome and its activity measured during the growth cycle...
  37. ncbi Characterization of the Escherichia coli gene for serine hydroxymethyltransferase
    M D Plamann
    Gene 22:9-18. 1983
    ..The transcription start points for the two genes are 216 bp apart, and the translation start sites are 327 bp apart. Less than 90 bp separate the two RNA polymerase molecules bound to the two promoters...
  38. ncbi A novel mechanism for upregulation of the Escherichia coli K-12 hmp (flavohaemoglobin) gene by the 'NO releaser', S-nitrosoglutathione: nitrosation of homocysteine and modulation of MetR binding to the glyA-hmp intergenic region
    J Membrillo-Hernandez
    The Krebs Institute for Biomolecular Research, Department of Molecular Biology and Biotechnology, The University of Sheffield, UK
    Mol Microbiol 29:1101-12. 1998
    The flavohaemoglobin gene, hmp, of Escherichia coli is upregulated by nitric oxide (NO) in a SoxRS-independent manner...
  39. ncbi Response of the NAD(P)H-oxidising flavohaemoglobin (Hmp) to prolonged oxidative stress and implications for its physiological role in Escherichia coli
    M F Anjum
    Krebs Institute for Biomolecular Research, Department of Molecular Biology and Biotechnology, University of Sheffield, UK
    FEMS Microbiol Lett 166:219-23. 1998
    The Escherichia coli flavohaemoglobin (Hmp) has a globin-like N-terminal domain and a ferredoxin-NADP-reductase-like C-terminal domain. We show here that purified Hmp oxidises both NADH and NADPH with Km values of 1.8 and 19...
  40. ncbi Isolation and nucleotide sequence of the hmp gene that encodes a haemoglobin-like protein in Escherichia coli K-12
    S G Vasudevan
    Division of Biochemistry and Molecular Biology, John Curtin School of Medical Research, Australian National University, Canberra, Australia
    Mol Gen Genet 226:49-58. 1991
    ..We conclude that E. coli produces a soluble haemoglobin-like protein, the product of the hmp gene (for haemoprotein). Although the protein has DHPR activity, it is distinct from the previously purified E...
  41. ncbi The haemoglobin-like protein (HMP) of Escherichia coli has ferrisiderophore reductase activity and its C-terminal domain shares homology with ferredoxin NADP+ reductases
    S C Andrews
    Krebs Institute for Biomolecular Research, Department of Molecular Biology and Biotechnology, University of Sheffield, UK
    FEBS Lett 302:247-52. 1992
    Three soluble ferrisiderophore reductases (FsrA, FsrB and FsrC) were detected in Escherichia coli. FsrB was purified and identified as the haemoglobin-like protein (HMP) by size and N-terminal sequence analyses...
  42. ncbi The flavohemoglobin of Escherichia coli confers resistance to a nitrosating agent, a "Nitric oxide Releaser," and paraquat and is essential for transcriptional responses to oxidative stress
    J Membrillo-Hernandez
    The Krebs Institute for Biomolecular Research, Department of Molecular Biology and Biotechnology, The University of Sheffield, Firth Court, Western Bank, Sheffield S10 2TN, United Kingdom
    J Biol Chem 274:748-54. 1999
    Escherichia coli possesses a flavohemoglobin (Hmp), product of hmp, the first microbial globin gene to be sequenced and characterized at the molecular level...
  43. ncbi Anoxic function for the Escherichia coli flavohaemoglobin (Hmp): reversible binding of nitric oxide and reduction to nitrous oxide
    S O Kim
    Krebs Institute for Biomolecular Research, Department of Molecular Biology and Biotechnology, The University of Sheffield, UK
    FEBS Lett 445:389-94. 1999
    The flavohaemoglobin Hmp of Escherichia coli is inducible by nitric oxide (NO) and provides protection both aerobically and anaerobically from inhibition of growth by NO and agents that cause nitrosative stress...
  44. ncbi Steady-state and transient kinetics of Escherichia coli nitric-oxide dioxygenase (flavohemoglobin). The B10 tyrosine hydroxyl is essential for dioxygen binding and catalysis
    A M Gardner
    Division of Critical Care Medicine, Children s Hospital Medical Center, Cincinnati, Ohio 45229, USA
    J Biol Chem 275:12581-9. 2000
    ..At 37 degrees C, V(max)/K(m)(NO) is 2,400 microM(-1) s(-1), demonstrating that the enzyme is extremely efficient at converting toxic NO into nitrate under physiological conditions...
  45. ncbi New functions for the ancient globin family: bacterial responses to nitric oxide and nitrosative stress
    R K Poole
    Krebs Institute for Biomolecular Research, Department of Molecular Biology and Biotechnology, The University of Sheffield, Firth Court, Western Bank, Sheffield S10 2TN, UK
    Mol Microbiol 36:775-83. 2000
    ..In Escherichia coli, the prototype flavohaemoglobin (Hmp) is clearly involved in responses to nitric oxide (NO) and nitrosative stress: (i) the structural gene hmp is ..
  46. ncbi The oxygenated flavohaemoglobin from Escherichia coli: evidence from photodissociation and rapid-scan studies for two kinetic and spectral forms
    Y Orii
    Department of Public Health, Faculty of Medicine, Kyoto University, Japan
    Biochem Biophys Res Commun 187:94-100. 1992
    The kinetics of dissociation and reassociation of the oxygenated species of Escherichia coli flavohaemoglobin (Hmp) were studied using stopped-flow rapid-scan and flash photolysis spectrophotometry at 25 degrees C...
  47. ncbi Bacterial hemoglobins and flavohemoglobins: versatile proteins and their impact on microbiology and biotechnology
    Alexander D Frey
    Institute of Biotechnology, ETH Zurich, 8093 Zurich, Switzerland
    FEMS Microbiol Rev 27:525-45. 2003
    ....
  48. ncbi Transcriptional responses of Escherichia coli to S-nitrosoglutathione under defined chemostat conditions reveal major changes in methionine biosynthesis
    Janet Flatley
    Department of Molecular Biology and Biotechnology, The University of Sheffield, Firth Court, Western Bank, Sheffield S10 2TN, UK
    J Biol Chem 280:10065-72. 2005
    ..only 17 genes were significantly up-regulated, including genes known to be involved in NO tolerance, particularly hmp (encoding the NO-consuming flavohemoglobin Hmp) and norV (encoding flavorubredoxin)...
  49. ncbi Interaction with membrane lipids and heme ligand binding properties of Escherichia coli flavohemoglobin
    Alessandra Bonamore
    CNR Istituto di Biologia e Patologia Molecolari and Department of Biochemical Sciences, University La Sapienza 00185 Rome, Italy
    Biochemistry 42:5792-801. 2003
    ..The effect of the bound lipid has been accounted for by a reaction scheme that involves the presence of two sites for the lipid/ligand recognition, namely, the heme iron and a non-heme site located in a loop region above the heme pocket...
  50. ncbi Constitutive and adaptive detoxification of nitric oxide in Escherichia coli. Role of nitric-oxide dioxygenase in the protection of aconitase
    P R Gardner
    Division of Critical Care Medicine, Children s Hospital Medical Center, Cincinnati, Ohio 45229, USA gardpo0chmcc org
    J Biol Chem 273:26528-33. 1998
    ..The latter activity was attributed to NOD. The results support a role for NOD in the aerobic detoxification of NO. and suggest functions for NOD and a cyanide-resistant NO. scavenging activity in anaerobic cells...
  51. pmc Nitrosative stress: metabolic pathway involving the flavohemoglobin
    A Hausladen
    Department of Medicine, Duke University Medical Center, Durham, NC 27710, USA
    Proc Natl Acad Sci U S A 95:14100-5. 1998
    ..Our results also emphasize that the reactions of SNO and NO with hemoglobins are evolutionary conserved, but have been adapted for cell-specific function...
  52. ncbi The distal heme pocket of Escherichia coli flavohemoglobin probed by infrared spectroscopy
    A Bonamore
    CNR Center of Molecular Biology, c o Department of Biochemical Sciences, University La Sapienza, P Aldo Moro 5, 00185, Rome, Italy
    Biochim Biophys Acta 1549:174-8. 2001
    ....
  53. ncbi Nitric-oxide dioxygenase activity and function of flavohemoglobins. sensitivity to nitric oxide and carbon monoxide inhibition
    P R Gardner
    Division of Critical Care Medicine, Children s Hospital Medical Center, Cincinnati, Ohio 45229, USA
    J Biol Chem 275:31581-7. 2000
    ..These results suggest that flavoHbs and related hemoglobins evolved as NO detoxifying components of nitrogen metabolism capable of discriminating O(2) from inhibitory NO and CO...