fur

Summary

Gene Symbol: fur
Description: ferric iron uptake regulon transcriptional repressor; autorepressor
Alias: ECK0671, JW0669
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. ncbi Metal binding characteristics and role of iron oxidation in the ferric uptake regulator from Escherichia coli
    Stephen A Mills
    Department of Chemistry, University of California, Berkeley, California 94720, USA
    Biochemistry 44:13553-9. 2005
  2. ncbi A method for direct cloning of fur-regulated genes: identification of seven new fur-regulated loci in Escherichia coli
    N Vassinova
    Department of Biophysics, St Petersburg State Technical University, 29 Polytechnicheskaya Str, St Petersburg 195251, Russia
    Microbiology 146:3171-82. 2000
  3. ncbi The ferric uptake regulation (Fur) repressor is a zinc metalloprotein
    E W Althaus
    Department of Chemistry, Northwestern University, Evanston, Illinois 60208 3113, USA
    Biochemistry 38:6559-69. 1999
  4. pmc A small RNA regulates the expression of genes involved in iron metabolism in Escherichia coli
    Eric Massé
    Laboratory of Molecular Biology, National Cancer Institute, Building 37, Room 5132, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 99:4620-5. 2002
  5. ncbi Fur-DNA interactions at the bidirectional fepDGC-entS promoter region in Escherichia coli
    Jennifer L Lavrrar
    Department of Molecular Microbiology and Immunology, University of Missouri Columbia, School of Medicine, 65212, USA
    J Mol Biol 322:983-95. 2002
  6. ncbi Iron and oxygen regulation of Escherichia coli MnSOD expression: competition between the global regulators Fur and ArcA for binding to DNA
    B Tardat
    Institut Jacques Monod, CNRS, Universite Paris 7, France
    Mol Microbiol 9:53-63. 1993
  7. pmc Oxidant-responsive induction of the suf operon, encoding a Fe-S assembly system, through Fur and IscR in Escherichia coli
    Kyung Chang Lee
    School of Biological Sciences, Seoul National University, Kwanak gu, Seoul 151 742, South Korea
    J Bacteriol 190:8244-7. 2008
  8. pmc Architecture of a fur binding site: a comparative analysis
    Jennifer L Lavrrar
    Department of Molecular Microbiology and Immunology, University of Missouri Columbia School of Medicine, Columbia, Missouri 65212, USA
    J Bacteriol 185:2194-202. 2003
  9. ncbi Global iron-dependent gene regulation in Escherichia coli. A new mechanism for iron homeostasis
    Jonathan P McHugh
    School of Animal and Microbial Sciences, University of Reading, Reading, RG6 6AJ, United Kingdom
    J Biol Chem 278:29478-86. 2003
  10. pmc Coupled degradation of a small regulatory RNA and its mRNA targets in Escherichia coli
    Eric Massé
    Laboratory of Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Genes Dev 17:2374-83. 2003

Scientific Experts

  • Eric Massé
  • Gregor Grass
  • A Kuzminov
  • Volkmar Braun
  • James Liao
  • Victor M Bolanos-Garcia
  • K Hantke
  • Francis Biville
  • James D Hoerter
  • Mazen Y Hamed
  • Ludmil Benov
  • Cécile Lelong
  • Robert K Poole
  • Isabelle Michaud-Soret
  • Benoît D'Autreaux
  • Branislav Vecerek
  • Jennifer L Lavrrar
  • Ludovic Pecqueur
  • Sang Woo Seo
  • Sagi Huja
  • Margarita M Arenas-Hernández
  • Beate Bersch
  • Alison I Graham
  • Guido Sanguinetti
  • Cameron W McLeod
  • Anjali Nandal
  • Mark P Brynildsen
  • Salih Jabour
  • William A Glover
  • Kelly S Davidge
  • Kyung Chang Lee
  • Mélanie L Guillemet
  • Steven T Pullan
  • Caroline Cuny
  • Elizabeth Hernández-Urzúa
  • Nadia Abed
  • Shery Varghese
  • Zehua Chen
  • Yuichi Otsuka
  • Sangho Chai
  • Jin Won Lee
  • Simon C Andrews
  • Francisco Rodríguez-Quiñones
  • Szabolcs Semsey
  • Eric Forest
  • Tara K Sigdel
  • Stephen A Mills
  • Udo Blasi
  • Zhongge Zhang
  • Isabella Moll
  • Ali Tiss
  • Elena A Kouzminova
  • A Adrait
  • A Gonzalez de Peredo
  • Jonathan P McHugh
  • Mark A McIntosh
  • Maricarmen Rojas-López
  • Haythem Latif
  • Susann Meyer
  • Eliora Z Ron
  • Abraham Medrano-López
  • Alfredo G Torres
  • Joerg Bernhard
  • Akira Katayama
  • Edward J O'Brien
  • Karen J Nuñez-Reza
  • Doerte Becher
  • Yaara Oren
  • Ulrich Dobrindt
  • Richard Szubin
  • Ygnacio Martinez-Laguna
  • Rotem Sorek
  • Donghyuk Kim
  • Jose Luis Puente
  • Dvora Biran
  • Bernhard O Palsson
  • Michael Hecker
  • Neil Bramall
  • T E Clarke
  • Mark R Woodhall
  • N Vassinova
  • Cerys C O Huggins
  • John R Guest
  • Jonathan McHugh
  • Michael A Quail
  • Chu Hoi Yee
  • Claire E Monk
  • Alan G Cox
  • Yanqin Yang
  • Ying Zhang

Detail Information

Publications71

  1. ncbi Metal binding characteristics and role of iron oxidation in the ferric uptake regulator from Escherichia coli
    Stephen A Mills
    Department of Chemistry, University of California, Berkeley, California 94720, USA
    Biochemistry 44:13553-9. 2005
    ..is believed to be the primary functional metal in vivo; however, the ability of other divalent cations to activate Fur brings into question the true physiological metal...
  2. ncbi A method for direct cloning of fur-regulated genes: identification of seven new fur-regulated loci in Escherichia coli
    N Vassinova
    Department of Biophysics, St Petersburg State Technical University, 29 Polytechnicheskaya Str, St Petersburg 195251, Russia
    Microbiology 146:3171-82. 2000
    A strain that allows the cloning of Fur-regulated loci was constructed. The strain, named FUR-SEL1, contains a chromosomal fhuA'-'cat transcriptional fusion that is expressed from the Fur-regulated promoter, fhuA(p)...
  3. ncbi The ferric uptake regulation (Fur) repressor is a zinc metalloprotein
    E W Althaus
    Department of Chemistry, Northwestern University, Evanston, Illinois 60208 3113, USA
    Biochemistry 38:6559-69. 1999
    The Fur protein regulates the expression of a wide variety of iron-responsive genes; however, the interaction of this repressor with its cognate metal ion remains controversial...
  4. pmc A small RNA regulates the expression of genes involved in iron metabolism in Escherichia coli
    Eric Massé
    Laboratory of Molecular Biology, National Cancer Institute, Building 37, Room 5132, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 99:4620-5. 2002
    ..proteins when iron is limiting; it is itself negatively regulated by the ferric uptake repressor protein, Fur (Ferric uptake regulator)...
  5. ncbi Fur-DNA interactions at the bidirectional fepDGC-entS promoter region in Escherichia coli
    Jennifer L Lavrrar
    Department of Molecular Microbiology and Immunology, University of Missouri Columbia, School of Medicine, 65212, USA
    J Mol Biol 322:983-95. 2002
    The transcriptional repressor Fur binds to a 19-bp consensus sequence, 5'-GATAATGATAATCATTATC-3', under high iron conditions. The fepDGC-entS promoter of Escherichia coli contains two Fur-binding sites (FBS) offset by 6bp...
  6. ncbi Iron and oxygen regulation of Escherichia coli MnSOD expression: competition between the global regulators Fur and ArcA for binding to DNA
    B Tardat
    Institut Jacques Monod, CNRS, Universite Paris 7, France
    Mol Microbiol 9:53-63. 1993
    ..in Escherichia coli, is negatively regulated by two global regulators, ArcA (aerobic respiration control) and Fur (ferric uptake regulation), coupling its expression to aerobic metabolism and the intracellular iron pool...
  7. pmc Oxidant-responsive induction of the suf operon, encoding a Fe-S assembly system, through Fur and IscR in Escherichia coli
    Kyung Chang Lee
    School of Biological Sciences, Seoul National University, Kwanak gu, Seoul 151 742, South Korea
    J Bacteriol 190:8244-7. 2008
    ..For apo-IscR to bind, oxidation-mediated dissociation of Fur is required...
  8. pmc Architecture of a fur binding site: a comparative analysis
    Jennifer L Lavrrar
    Department of Molecular Microbiology and Immunology, University of Missouri Columbia School of Medicine, Columbia, Missouri 65212, USA
    J Bacteriol 185:2194-202. 2003
    b>Fur is an iron-binding transcriptional repressor that recognizes a 19-bp consensus site of the sequence 5'-GATAATGATAATCATTATC-3'...
  9. ncbi Global iron-dependent gene regulation in Escherichia coli. A new mechanism for iron homeostasis
    Jonathan P McHugh
    School of Animal and Microbial Sciences, University of Reading, Reading, RG6 6AJ, United Kingdom
    J Biol Chem 278:29478-86. 2003
    ..This Fe-dependent repression is mediated by a transcriptional repressor, Fur (ferric uptake regulation), which also controls genes involved in other processes such as iron storage, the ..
  10. pmc Coupled degradation of a small regulatory RNA and its mRNA targets in Escherichia coli
    Eric Massé
    Laboratory of Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Genes Dev 17:2374-83. 2003
    RyhB is a small antisense regulatory RNA that is repressed by the Fur repressor and negatively regulates at least six mRNAs encoding Fe-binding or Fe-storage proteins in Escherichia coli...
  11. doi Induction of the ferritin gene (ftnA) of Escherichia coli by Fe(2+)-Fur is mediated by reversal of H-NS silencing and is RyhB independent
    Anjali Nandal
    School of Biological Sciences, University of Reading, Whiteknights, Reading RG6 6AJ, UK
    Mol Microbiol 75:637-57. 2010
    ..The FtnA gene (ftnA) is induced by iron in an Fe(2+)-Fur-dependent fashion. This effect is reportedly mediated by RyhB, the Fe(2+)-Fur-repressed, small, regulatory RNA...
  12. ncbi Ferric uptake regulation protein acts as a repressor, employing iron (II) as a cofactor to bind the operator of an iron transport operon in Escherichia coli
    A Bagg
    Department of Biochemistry, University of California, Berkeley 94720
    Biochemistry 26:5471-7. 1987
    The Fur (ferric uptake regulation) protein is a negative regulator of the aerobactin operon and of several other siderophore-mediated, high-affinity iron transport systems in Escherichia coli...
  13. pmc Control of Escherichia coli superoxide dismutase (sodA and sodB) genes by the ferric uptake regulation (fur) locus
    E C Niederhoffer
    Isotope and Structural Chemistry Group INC 4, Los Alamos, National Laboratory, New Mexico 87545
    J Bacteriol 172:1930-8. 1990
    The ferric uptake regulation (fur) gene product participates in regulating expression of the manganese- and iron-containing superoxide dismutase genes of Escherichia coli...
  14. pmc Genetic regulation of fluxes: iron homeostasis of Escherichia coli
    Szabolcs Semsey
    Center for Models of Life, Niels Bohr Institute, Copenhagen, Denmark
    Nucleic Acids Res 34:4960-7. 2006
    ..We suggest that Fe-S cluster assembly is crucial to prevent the accumulation of toxic levels of free intracellular iron when the environment suddenly becomes iron rich...
  15. pmc Effect of RyhB small RNA on global iron use in Escherichia coli
    Eric Massé
    Laboratory of Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, USA
    J Bacteriol 187:6962-71. 2005
    RyhB is a noncoding RNA regulated by the Fur repressor. It has previously been shown to cause the rapid degradation of a number of mRNAs that encode proteins that utilize iron...
  16. ncbi Ironing out the problem: new mechanisms of iron homeostasis
    Eric Massé
    Universite de Sherbrooke, Departement de Biochimie, Quebec, Canada
    Trends Biochem Sci 30:462-8. 2005
    ..The common characteristics of these three different organisms suggest a novel mechanism of iron homeostasis...
  17. ncbi Fur (ferric uptake regulation) protein and CAP (catabolite-activator protein) modulate transcription of fur gene in Escherichia coli
    V de Lorenzo
    Department of Biochemistry, University of California, Berkeley 94720
    Eur J Biochem 173:537-46. 1988
    A fusion between the fur (ferric uptake regulation) gene, known to mediate negative regulation of iron absorption in Escherichia coli, and lacZ was constructed in vitro...
  18. pmc OxyR and SoxRS regulation of fur
    M Zheng
    Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Bacteriol 181:4639-43. 1999
    ..that regulators of the Escherichia coli responses to oxidative stress, OxyR and SoxRS, activate the expression of Fur, the global repressor of ferric ion uptake...
  19. pmc Deciphering Fur transcriptional regulatory network highlights its complex role beyond iron metabolism in Escherichia coli
    Sang Woo Seo
    1 Department of Bioengineering, University of California San Diego, La Jolla, California 92093 0412, USA 2
    Nat Commun 5:4910. 2014
    The ferric uptake regulator (Fur) plays a critical role in the transcriptional regulation of iron metabolism. However, the full regulatory potential of Fur remains undefined...
  20. pmc Fur is the master regulator of the extraintestinal pathogenic Escherichia coli response to serum
    Sagi Huja
    Faculty of Life Sciences, Tel Aviv University, Tel Aviv, Israel
    MBio 5:. 2014
    ..In particular, the serum metabolic response is coordinated by three transcriptional regulators, Fur, BasR, and CysB. Fur alone was responsible for more than 80% of the serum-induced transcriptional response...
  21. pmc Environmental regulation of the long polar fimbriae 2 of enterohemorrhagic Escherichia coli O157:H7
    Margarita M Arenas-Hernández
    Posgrado en Microbiología, Benemerita Universidad Autonoma de Puebla, Puebla, Mexico Licenciatura en Biomedicina, Benemerita Universidad Autonoma de Puebla, Puebla, Mexico Centro de Investigaciones en Ciencias Microbiológicas, Benemerita Universidad Autonoma de Puebla, Puebla, Mexico
    FEMS Microbiol Lett 357:105-14. 2014
    ..expression was decreased under iron-depleted conditions, its expression was increased in a ferric-uptake-regulator (Fur) mutant strain. The lpfA2 transcript was 0.7 and 2 times more abundant in wt EHEC grown in DMEM pH 6...
  22. doi Dynamics of a starvation-to-surfeit shift: a transcriptomic and modelling analysis of the bacterial response to zinc reveals transient behaviour of the Fur and SoxS regulators
    Alison I Graham
    Department of Molecular Biology and Biotechnology, The University of Sheffield, Western Bank, Sheffield S10 2TN, UK
    Microbiology 158:284-92. 2012
    ..transcriptomic data generated from the Zn shift focused on the role of ten key regulators; ArsR, BaeR, CpxR, CusR, Fur, OxyR, SoxS, ZntR, ZraR and Zur...
  23. pmc Insights into the molecular basis of L-form formation and survival in Escherichia coli
    William A Glover
    Department of Molecular Microbiology and Immunology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, Maryland, United States of America
    PLoS ONE 4:e7316. 2009
    ..a positive response regulator of colanic acid capsule synthesis, ruvA, a recombinational junction binding protein, fur, a ferric uptake regulator and smpA a small membrane lipoprotein were selected for complementation...
  24. pmc RecA-dependent mutants in Escherichia coli reveal strategies to avoid chromosomal fragmentation
    Elena A Kouzminova
    Department of Microbiology, University of Illinois at Urbana Champaign, Urbana, IL 61801 3709, USA
    Proc Natl Acad Sci U S A 101:16262-7. 2004
    ..The tdk and rdgB mutants affect synthesis of DNA precursors. The fur, ubiE, and ubiH mutants are likely to have increased levels of reactive oxygen species...
  25. ncbi Iron(II) triggered conformational changes in Escherichia coli fur upon DNA binding: a study using molecular modeling
    Mazen Y Hamed
    Computational Science Program, Chemistry Department, Birzeit University, PO Box 14, Birzeit, Palestine
    J Mol Graph Model 25:234-46. 2006
    In order to identify the Fur dimerization domain, a three-dimensional structure of the ferric uptake regulation protein from Escherichia coli (Fur EC) was determined using homology modeling and energy minimization...
  26. ncbi Structural changes of Escherichia coli ferric uptake regulator during metal-dependent dimerization and activation explored by NMR and X-ray crystallography
    Ludovic Pecqueur
    Laboratoire de Résonance Magnétique Nucléaire des Protéines and Laboratoire de Cristallographie et de Cristallogénèse des Protéines, Grenoble, France
    J Biol Chem 281:21286-95. 2006
    Ferric uptake regulator (Fur) is a global bacterial regulator that uses iron as a cofactor to bind to specific DNA sequences. Escherichia coli Fur is usually isolated as a homodimer with two metal sites per subunit...
  27. ncbi Structural analysis and classification of native proteins from E. coli commonly co-purified by immobilised metal affinity chromatography
    Victor Martin Bolanos-Garcia
    Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Cambridge CB2 1GA, England
    Biochim Biophys Acta 1760:1304-13. 2006
    ..The work presented here reviews the native proteins from E. coli most commonly co-purified by IMAC, including Fur, Crp, ArgE, SlyD, GlmS, GlgA, ODO1, ODO2, YadF and YfbG...
  28. pmc Transcriptional response of Escherichia coli to TPEN
    Tara K Sigdel
    Department of Chemistry and Biochemistry, 160 Hughes Hall, Miami University, Oxford, OH 45056, USA
    J Bacteriol 188:6709-13. 2006
    ..Fifty-five transcripts were significantly up-regulated, including all of the genes that are regulated by Zur and many that are regulated by Fur. In the same TPEN-treated cells, 46 transcripts were significantly down-regulated.
  29. ncbi Multiple regulators of the Flavohaemoglobin (hmp) gene of Salmonella enterica serovar Typhimurium include RamA, a transcriptional regulator conferring the multidrug resistance phenotype
    Elizabeth Hernández-Urzúa
    Laboratorio de Microbiología y Genética Molecular, Departamento de Biologia Molecular y Biotecnologia, Instituto de Investigaciones Biomedicas, Universidad Nacional Autonoma de Mexico, PO Box 70 228, Coyoacan, Mexico City 04510, Mexico
    Arch Microbiol 187:67-77. 2007
    ..and Salmonella modest transcriptional repression of hmp is exerted by the iron responsive transcriptional repressor Fur. Finally, in contrast to previous reports, we show that in E...
  30. pmc Induction of a global stress response during the first step of Escherichia coli plate growth
    Caroline Cuny
    Laboratoire de Chimie Bacterienne, UPR 9043, CNRS, 31, Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
    Appl Environ Microbiol 73:885-9. 2007
    ..several global regulons, like heat shock regulons (RpoH, RpoE, CpxAR) and oxidative-stress regulons (SoxRS, OxyR, Fur), were immediately induced...
  31. ncbi Functional specialization within the Fur family of metalloregulators
    Jin Won Lee
    Department of Microbiology, Cornell University, Wing Hall, Ithaca, NY 14853 8101, USA
    Biometals 20:485-99. 2007
    The ferric uptake regulator (Fur) protein, as originally described in Escherichia coli, is an iron-sensing repressor that controls the expression of genes for siderophore biosynthesis and iron transport...
  32. pmc Discovery of Fur binding site clusters in Escherichia coli by information theory models
    Zehua Chen
    National Cancer Institute at Frederick, Center for Cancer Research Nanobiology Program, Basic Research Program, SAIC Frederick, Inc, Frederick, MD 21702 1201, USA
    Nucleic Acids Res 35:6762-77. 2007
    b>Fur is a DNA binding protein that represses bacterial iron uptake systems...
  33. ncbi Mutual regulation of Crl and Fur in Escherichia coli W3110
    Cécile Lelong
    Laboratoire Adaptation et Pathogenie des Microorganismes, Institut Jean Roget, Domaine de la Merci, Universite Joseph Fourier, BP 170, 38042 Grenoble Cedex 9, France
    Mol Cell Proteomics 6:660-8. 2007
    ..showed that Crl regulates the expression of another global regulator, the central regulator of iron homeostasis, Fur. A molecular analysis revealed that Crl and Fur affect their own and each other's expression...
  34. doi Carbon monoxide-releasing antibacterial molecules target respiration and global transcriptional regulators
    Kelly S Davidge
    Department of Molecular Biology and Biotechnology, The University of Sheffield, Firth Ct, Western Bank, Sheffield S10 2TN, United Kingdom
    J Biol Chem 284:4516-24. 2009
    ..Probabilistic modeling of transcriptomic data identified the global transcription regulators ArcA, CRP, Fis, FNR, Fur, BaeR, CpxR, and IHF as targets and potential CO sensors...
  35. ncbi Reversible redox- and zinc-dependent dimerization of the Escherichia coli fur protein
    Benoît D'Autreaux
    Laboratoire de Physicochimie des Métaux en Biologie, UMR 5155 CNRS CEA UJF, Departement Reponse et Dynamique Cellulaires, CEA Grenoble, 17 Avenue des Martyrs, 38054 Grenoble Cedex 9, France
    Biochemistry 46:1329-42. 2007
    b>Fur is a bacterial regulator using iron as a cofactor to bind to specific DNA sequences. This protein exists in solution as several oligomeric states, of which the dimer is generally assumed to be the biologically relevant one...
  36. doi Binding of the Zn2+ ion to ferric uptake regulation protein from E. coli and the competition with Fe2+ binding: a molecular modeling study of the effect on DNA binding and conformational changes of Fur
    Salih Jabour
    Computational Science Program, Chemistry Department, Birzeit University, P O Box 14, Birzeit, Palestine
    J Comput Aided Mol Des 23:199-208. 2009
    ..Fe2+, when added to the complex consisting of 2Zn2+/Fur dimer/DNA, replaced the Zn2+ ion in the zinc site and when a second Fe2+ was added, it replaced the second zinc ion ..
  37. pmc Control of Fur synthesis by the non-coding RNA RyhB and iron-responsive decoding
    Branislav Vecerek
    Department of Microbiology and Immunobiology, Max F Perutz Laboratories, University of Vienna, Vienna, Austria
    EMBO J 26:965-75. 2007
    The Fe2+-dependent Fur protein serves as a negative regulator of iron uptake in bacteria. As only metallo-Fur acts as an autogeneous repressor, Fe2+scarcity would direct fur expression when continued supply is not obviously required...
  38. pmc Fur-dependent detoxification of organic acids by rpoS mutants during prolonged incubation under aerobic, phosphate starvation conditions
    Mélanie L Guillemet
    Laboratoire de Chimie Bacterienne, Centre National de la Recherche Scientifique, 13009 Marseille, France
    J Bacteriol 190:5567-75. 2008
    ..The characteristic metabolism of rpoS mutants required the activity of Fur (ferric uptake regulator) in order to decrease the synthesis of the small RNA RyhB that might otherwise inhibit the ..
  39. pmc Submicromolar hydrogen peroxide disrupts the ability of Fur protein to control free-iron levels in Escherichia coli
    Shery Varghese
    Department of Microbiology, University of Illinois, Urbana, IL 61801, USA
    Mol Microbiol 64:822-30. 2007
    ..We observed that in defined medium these strains constitutively expressed members of the Fur regulon. Iron-import proteins, which Fur normally represses, were fully induced...
  40. ncbi A comparative analysis of perturbations caused by a gene knock-out, a dominant negative allele, and a set of peptide aptamers
    Nadia Abed
    Differentiation and Cell Cycle Group, Laboratoire de Biologie Moleculaire de la Cellule, UMR 5239 CNRS ENS Lyon, Universite Lyon 1, Ecole Normale Superieure de Lyon, IFR 128 Biosciences Lyon Gerland, 46 allee d Italie, 69364 Lyon Cedex 07, France
    Mol Cell Proteomics 6:2110-21. 2007
    ..Here we used the Escherichia coli global transcription regulator Fur (ferric uptake regulator) as a case study to compare the perturbations exerted by a gene knock-out, the expression ..
  41. ncbi Effect of fur on pyrC gene expression
    Sangho Chai
    Department of Biomedical Sciences, Hallym University, Chun Cheon, Republic of Korea
    J Microbiol 45:583-9. 2007
    The promoter region of pyrC (dihydroorotase) gene of Escherichia coli was shown to have Fur protein binding properties by gel retardation assay...
  42. ncbi A role of RnlA in the RNase LS activity from Escherichia coli
    Yuichi Otsuka
    Department of Biological Sciences, Graduate School of Science, Osaka University, Osaka, Japan
    Genes Genet Syst 82:291-9. 2007
    ..Among these, triose phosphate isomerase exhibited a remarkable affinity to RnlA. These results suggest that RnlA plays a central role in RNase LS activity and that its activity is regulated by multiple components...
  43. ncbi Structural biology of bacterial iron uptake systems
    T E Clarke
    Department of Biological Sciences, University of Calgary, Alberta, Canada
    Curr Top Med Chem 1:7-30. 2001
    ..Likewise, the iron-sensitive gene regulatory protein Fur is found in most bacteria...
  44. ncbi Structural dynamics and functional domains of the fur protein
    M Coy
    Department of Molecular and Cellular Biology, University of California, Berkeley 94720
    Biochemistry 30:8201-10. 1991
    Proteolytic enzymes were used to detect metal-induced conformational changes in the ferric uptake regulation (Fur) protein of Escherichia coli K12...
  45. ncbi The binding of the ferric uptake regulation protein to a DNA fragment
    T Saito
    Inorganic Chemistry Laboratory, University of Oxford, England
    Eur J Biochem 197:43-7. 1991
    ..NMR, we have studied the binding of a DNA fragment in double-stranded form to the ferric uptake regulation protein, Fur. We have also looked at the binding of [Cr(CN)6]3- to Fur with a view to testing whether binding is due to ..
  46. ncbi Two global regulators repress the anaerobic expression of MnSOD in Escherichia coli::Fur (ferric uptake regulation) and Arc (aerobic respiration control)
    B Tardat
    Institut Jacques Monod, CNRS, Universite Paris 7, France
    Mol Microbiol 5:455-65. 1991
    ..expressing sodA in the absence of oxygen has revealed that mutations in genes for two global regulatory systems, Fur (ferric uptake regulation) and Arc (aerobic respiration control), are simultaneously required for the expression of ..
  47. ncbi Regulation of bacterial gene expression by metal-protein complexes
    H Hennecke
    Mikrobiologisches Institut, Eidgenossische Technische Hochschule, ETH Zentrum, Zurich, Switzerland
    Mol Microbiol 4:1621-8. 1990
    ..Other metal-dependent regulatory proteins may function, directly or indirectly, as sensors of the cellular oxygen status, and may even be mediators in light-responsive gene regulation...
  48. ncbi Expression, isolation and properties of Fur (ferric uptake regulation) protein of Escherichia coli K 12
    S Wee
    Department of Biochemistry, University of California, Berkeley 94720
    Biol Met 1:62-8. 1988
    The cloned fur (ferric uptake regulation) gene of Escherichia coli K12 was ligated to an expression vector which was inducible with nalidixic acid...
  49. pmc Mapping of a mutation affecting regulation of iron uptake systems in Escherichia coli K-12
    A Bagg
    J Bacteriol 161:450-3. 1985
    ..The site of a mutation resulting in constitutive derepression of iron uptake systems has been localized at 15.7 min on the genetic map of Escherichia coli K-12...
  50. ncbi Cloning of the repressor protein gene of iron-regulated systems in Escherichia coli K12
    K Hantke
    Mol Gen Genet 197:337-41. 1984
    In Escherichia coli the iron uptake systems are regulated by the fur gene product...
  51. ncbi Protein-induced bending as a transcriptional switch
    J Perez-Martin
    Centro de Investigaciones Biologicas, Consejo Superior de Investigaciones Cientificas, Madrid, Spain
    Science 260:805-7. 1993
    ..The catabolite activating protein binding site of the fur operon was replaced by the binding site of the RepA repressor protein, which is able to bend DNA immediately after ..
  52. ncbi Role of enterobactin and intracellular iron in cell lethality during near-UV irradiation in Escherichia coli
    J Hoerter
    Department of Biological Sciences, Ferris State University, Big Rapids, MI 49307 2225, USA
    Photochem Photobiol 64:537-41. 1996
    In Escherichia coli, fur mutants that constitutively express their native iron chelating agent, enterobactin, are significantly more sensitive to near-UV radiation (NUV) than wild type, An entA mutant, which is incapable of synthesizing ..
  53. ncbi Spectroscopic and saturation magnetization properties of the manganese- and cobalt-substituted Fur (ferric uptake regulation) protein from Escherichia coli
    A Adrait
    Laboratoire de Chimie de Coordination Unité de Recherche Associée au CNRS No 1194, Service de Chimie Inorganique et Biologique, Departement de Recherche Fondamentale sur la Matiere Condensee, CEA Grenoble, 38054 Grenoble Cedex 9, France
    Biochemistry 38:6248-60. 1999
    The Fur apoprotein has been purified and reconstituted with Co2+ and Mn2+ ions. These samples have been analyzed by UV-visible, EPR, and 1H NMR spectroscopies, by XAS, and by magnetization measurements...
  54. ncbi Characterization of the DNA-binding site in the ferric uptake regulator protein from Escherichia coli by UV crosslinking and mass spectrometry
    Ali Tiss
    Laboratoire de Spectrométrie de Masse des Protéines, Institut de Biologie Structurale UMR 5075 CEA CNRS UJF, Grenoble, France
    FEBS Lett 579:5454-60. 2005
    Ferric uptake regulator protein (Fur) is activated by its cofactor iron to a state that binds to a specific DNA sequence called 'Fur box'...
  55. ncbi Members of the Fur protein family regulate iron and zinc transport in E. coli and characteristics of the Fur-regulated fhuF protein
    Klaus Hantke
    Mikrobiologie Membranphysiologie, Universitat Tubingen, Germany
    J Mol Microbiol Biotechnol 4:217-22. 2002
    The regulator Fur represses with Fe2+ as cofactor iron uptake genes. The fhuF gene reacts very sensitive to minor changes of Fe2+ and Fur...
  56. ncbi Systematic search for zinc-binding proteins in Escherichia coli
    Akira Katayama
    National Institute of Genetics, Department of Molecular Genetics, Mishima, Shizuoka, Japan
    Eur J Biochem 269:2403-13. 2002
    ..coli increased up to more than 30 species (or more than 3% of about 1000 proteins expressed under laboratory culture conditions). The specificity and affinity of zinc-binding were analysed for some of the zinc-binding proteins...
  57. pmc Direct inhibition by nitric oxide of the transcriptional ferric uptake regulation protein via nitrosylation of the iron
    Benoît D'Autreaux
    Laboratoire de Physicochimie des Métaux en Biologie Formation de Recherche en Evolution Université Joseph Fourier Commissariat à l Energie Atomique Centre National de la Recherche Scientifique no 2427, Grenoble, F 38054 Grenoble Cedex 9, France
    Proc Natl Acad Sci U S A 99:16619-24. 2002
    Ferric uptake regulation protein (Fur) is a bacterial global regulator that uses iron as a cofactor to bind to specific DNA sequences. The function of Fur is not limited to iron homeostasis...
  58. ncbi Escherichia coli response to exogenous pyrophosphate and analogs
    Francis Biville
    Département de Biochimie et Génétique moléculaire, Institut Pasteur, Paris, France
    J Mol Microbiol Biotechnol 5:37-45. 2003
    ..of iron had the same positive effects as pyrophosphate on growth yield, cAMP synthesis and the repression of Fur-regulated genes. In contrast, imidodiphosphate did not affect these cellular processes significantly...
  59. ncbi Iron uptake by Escherichia coli
    Volkmar Braun
    Mikrobiologie Membranphysiologie, Universitat Tubingen, Auf der Morgenstelle 28, D 72076 Tubingen, Germany
    Front Biosci 8:s1409-21. 2003
    ..Transcription of the transport protein genes is regulated by the Fur protein, which when loaded with ferrous iron functions as a repressor...
  60. ncbi Interaction of the RNA chaperone Hfq with mRNAs: direct and indirect roles of Hfq in iron metabolism of Escherichia coli
    Branislav Vecerek
    Max F Perutz Laboratories, Department of Microbiology and Genetics, University Department at the Vienna Biocenter, Dr Bohrgasse 9, 1030 Vienna, Austria
    Mol Microbiol 50:897-909. 2003
    ..The effect of Hfq on some of the corresponding mRNAs including fur, gapA, metF, ppiB and sodB mRNA was assessed, using different in vitro and in vivo methods...
  61. ncbi Escherichia coli deltafur mutant displays low HPII catalase activity in stationary phase
    Ludmil Benov
    Department of Biochemistry, Faculty of Medicine, Kuwait University, Kuwait
    Redox Rep 8:379-83. 2003
    ..In Escherichia coli, iron metabolism is regulated by the Fur protein...
  62. pmc Linkage between catecholate siderophores and the multicopper oxidase CueO in Escherichia coli
    Gregor Grass
    Department of Soil, Water, and Environmental Science, University of Arizona, Shantz Bldg 38, Rm 424, Tucson, AZ 85721, USA
    J Bacteriol 186:5826-33. 2004
    ..coli cells, but the addition of purified CueO led to their survival. Deletion of fur resulted in copper hypersensitivity that was alleviated by additional deletion of entC, preventing synthesis of ..
  63. pmc Regulatory roles of Fnr, Fur, and Arc in expression of manganese-containing superoxide dismutase in Escherichia coli
    H M Hassan
    Department of Biochemistry, North Carolina State University, Raleigh 27695 7622
    Proc Natl Acad Sci U S A 89:3217-21. 1992
    ..Strains harboring simultaneous mutations in arcA/B and fur expressed sodA-lacZ under anaerobic growth conditions but were still inducible by iron chelators...
  64. ncbi FieF (YiiP) from Escherichia coli mediates decreased cellular accumulation of iron and relieves iron stress
    Gregor Grass
    Institut fur Mikrobiologie, Martin Luther Universität Halle, Kurt Mothes Str 3, Halle 06120, Germany
    Arch Microbiol 183:9-18. 2005
    ..with iron concentration; however, expression appeared to be independent of the ferrous iron uptake regulator Fur. Absence of FieF led to decreased growth of E...
  65. pmc Functional interactions between the carbon and iron utilization regulators, Crp and Fur, in Escherichia coli
    Zhongge Zhang
    Division of Biological Sciences, University of California at San Diego, La Jolla, CA 92093 0116, USA
    J Bacteriol 187:980-90. 2005
    In Escherichia coli, the ferric uptake regulator (Fur) controls expression of the iron regulon in response to iron availability while the cyclic AMP receptor protein (Crp) regulates expression of the carbon regulon in response to carbon ..
  66. ncbi Reduced hydroperoxidase (HPI and HPII) activity in the Deltafur mutant contributes to increased sensitivity to UVA radiation in Escherichia coli
    James D Hoerter
    Department of Biological Sciences, Ferris State University, Big Rapids, MI 49307, USA
    J Photochem Photobiol B 79:151-7. 2005
    ..hydroxyl radical (HO.), hydrogen peroxide (H2O2) and singlet oxygen (1O2). There is accumulating evidence that Fur may play an important role in the defense against UVA radiation...
  67. ncbi Identification of the two zinc-bound cysteines in the ferric uptake regulation protein from Escherichia coli: chemical modification and mass spectrometry analysis
    A Gonzalez de Peredo
    Laboratoire de Spectrométrie de Masse des Protéines, Institut de Biologie Structurale, 38027 Grenoble Cedex 1, France
    Biochemistry 38:8582-9. 1999
    ..combined with mass spectrometry analysis was used to characterize cysteine ligands in the zinc-binding site of the Fur protein...
  68. pmc Nitric oxide in chemostat-cultured Escherichia coli is sensed by Fnr and other global regulators: unaltered methionine biosynthesis indicates lack of S nitrosation
    Steven T Pullan
    Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield S10 2TN, UK
    J Bacteriol 189:1845-55. 2007
    ..Other global regulators apparently affected by NO were IscR, Fur, SoxR, NsrR, and NorR...
  69. pmc An integrated network approach identifies the isobutanol response network of Escherichia coli
    Mark P Brynildsen
    Department of Chemical and Biomolecular Engineering, University of California, Los Angeles, CA 90095, USA
    Mol Syst Biol 5:277. 2009
    ..Quinone/quinol malfunction was postulated to activate ArcA, Fur, and PhoB in this study...
  70. ncbi Nucleotide sequence of the iron regulatory gene fur
    S Schaffer
    Mol Gen Genet 200:110-3. 1985
    The fur gene of Escherichia coli is involved in all iron-regulated transcriptions hitherto studied. The nucleotide sequence of an 868 basepair fragment containing the fur gene was determined. There was only a single longer reading frame...

Research Grants5

  1. Automated Chip-Based Metabolomic Analysis
    James Liao; Fiscal Year: 2006
    ..abstract_text> ..
  2. Protein Oxidation in Skin Cells by Tan Bed Irradiation
    JAMES HOERTER; Fiscal Year: 2006
    ..abstract_text> ..
  3. Engineering Large scale pathways from organisms to Escherichia coli
    James Liao; Fiscal Year: 2008
    ..In particular, selected projects proposed here will be used as modules in a workshop established by FRT at Ohio State and form the basis for a course (Metabolic Engineering) at UCLA. ..
  4. Chemogenomic Analysis of E. coli Response to NO species
    James C Liao; Fiscal Year: 2010
    ..If hew TF targets are predicted, then the iteration continues. Otherwise, the predicted networks are verified using genetic and biochemical experiments. ..
  5. Nitric Oxide Interaction with Red Blood Cells
    James C Liao; Fiscal Year: 2010
    ..Specific aim 2 will focus on the functional roles of this regulation using isolated porcine pulmonary and coronary microvessels. Together, these results will suggest clinical relevance and potential interventions. ..