ftsZ

Summary

Gene Symbol: ftsZ
Description: GTP-binding tubulin-like cell division protein
Alias: ECK0096, JW0093, sfiB, sulB
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. pmc A gain-of-function mutation in ftsA bypasses the requirement for the essential cell division gene zipA in Escherichia coli
    Brett Geissler
    Department of Microbiology and Molecular Genetics, University of Texas Medical School, 6431 Fannin Street, Houston, TX 77030, USA
    Proc Natl Acad Sci U S A 100:4197-202. 2003
  2. pmc Genetic and functional analyses of the conserved C-terminal core domain of Escherichia coli FtsZ
    X Ma
    Department of Microbiology and Molecular Genetics, University of Texas Houston Medical School, Houston, Texas 77030, USA
    J Bacteriol 181:7531-44. 1999
  3. pmc Assembly of an FtsZ mutant deficient in GTPase activity has implications for FtsZ assembly and the role of the Z ring in cell division
    A Mukherjee
    Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City, Kansas 66160, USA
    J Bacteriol 183:7190-7. 2001
  4. pmc Inactivation of FtsI inhibits constriction of the FtsZ cytokinetic ring and delays the assembly of FtsZ rings at potential division sites
    J Pogliano
    Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 94:559-64. 1997
  5. pmc Domain folding and flexibility of Escherichia coli FtsZ determined by tryptophan site-directed mutagenesis
    Rodrigo Diaz-Espinoza
    Laboratorio de Biología Estructural y Molecular, Departamento de Biologia, Facultad de Ciencias, Universidad de Chile, Casilla 653, Santiago, Chile
    Protein Sci 16:1543-56. 2007
  6. ncbi The essential bacterial cell-division protein FtsZ is a GTPase
    P de Boer
    Department of Microbiology, University of Connecticut Health Center, Farmington 06030
    Nature 359:254-6. 1992
  7. pmc The MinC component of the division site selection system in Escherichia coli interacts with FtsZ to prevent polymerization
    Z Hu
    Department of Microbiology, University of Kansas Medical Center, Kansas City, KS 66160, USA
    Proc Natl Acad Sci U S A 96:14819-24. 1999
  8. ncbi The order of the ring: assembly of Escherichia coli cell division components
    Miguel Vicente
    Centro Nacional de Biotecnologia, Consejo Superior de Investigaciones Cientificas, Campus de Cantoblanco, C Darwin 3, 28049 Madrid, Spain
    Mol Microbiol 61:5-8. 2006
  9. pmc ZipA is a MAP-Tau homolog and is essential for structural integrity of the cytokinetic FtsZ ring during bacterial cell division
    D RayChaudhuri
    Department of Molecular Biology and Microbiology, Tufts University School of Medicine, 136 Harrison Avenue, Boston, MA 02111, USA
    EMBO J 18:2372-83. 1999
  10. pmc ZipA-induced bundling of FtsZ polymers mediated by an interaction between C-terminal domains
    C A Hale
    Department of Molecular Biology and Microbiology, Case Western Reserve University School of Medicine, Cleveland, Ohio 44106 4960, USA
    J Bacteriol 182:5153-66. 2000

Research Grants

Scientific Experts

Detail Information

Publications135 found, 100 shown here

  1. pmc A gain-of-function mutation in ftsA bypasses the requirement for the essential cell division gene zipA in Escherichia coli
    Brett Geissler
    Department of Microbiology and Molecular Genetics, University of Texas Medical School, 6431 Fannin Street, Houston, TX 77030, USA
    Proc Natl Acad Sci U S A 100:4197-202. 2003
    ZipA and FtsA are recruited independently to the FtsZ cytokinetic ring (Z ring) and are essential for cell division of Escherichia coli...
  2. pmc Genetic and functional analyses of the conserved C-terminal core domain of Escherichia coli FtsZ
    X Ma
    Department of Microbiology and Molecular Genetics, University of Texas Houston Medical School, Houston, Texas 77030, USA
    J Bacteriol 181:7531-44. 1999
    In Escherichia coli, FtsZ is required for the recruitment of the essential cell division proteins FtsA and ZipA to the septal ring. Several C-terminal deletions of E...
  3. pmc Assembly of an FtsZ mutant deficient in GTPase activity has implications for FtsZ assembly and the role of the Z ring in cell division
    A Mukherjee
    Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City, Kansas 66160, USA
    J Bacteriol 183:7190-7. 2001
    b>FtsZ, the ancestral homologue of eukaryotic tubulins, assembles into the Z ring, which is required for cytokinesis in prokaryotic cells. Both FtsZ and tubulin have a GTPase activity associated with polymerization...
  4. pmc Inactivation of FtsI inhibits constriction of the FtsZ cytokinetic ring and delays the assembly of FtsZ rings at potential division sites
    J Pogliano
    Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 94:559-64. 1997
    ..In the bacterium Escherichia coli, this ring is formed by the essential cell division protein FtsZ. We have used immunofluorescence microscopy to show that FtsZ assembles early in the division cycle, suggesting ..
  5. pmc Domain folding and flexibility of Escherichia coli FtsZ determined by tryptophan site-directed mutagenesis
    Rodrigo Diaz-Espinoza
    Laboratorio de Biología Estructural y Molecular, Departamento de Biologia, Facultad de Ciencias, Universidad de Chile, Casilla 653, Santiago, Chile
    Protein Sci 16:1543-56. 2007
    b>FtsZ has two domains, the amino GTPase domain with a Rossmann fold, and the carboxyl domain that resembles the chorismate mutase fold...
  6. ncbi The essential bacterial cell-division protein FtsZ is a GTPase
    P de Boer
    Department of Microbiology, University of Connecticut Health Center, Farmington 06030
    Nature 359:254-6. 1992
    ..The biochemical mechanisms responsible for this process are poorly understood. In bacteria, the ftsZ gene product, FtsZ, is required for cell division, playing a prominent role in cytokinesis...
  7. pmc The MinC component of the division site selection system in Escherichia coli interacts with FtsZ to prevent polymerization
    Z Hu
    Department of Microbiology, University of Kansas Medical Center, Kansas City, KS 66160, USA
    Proc Natl Acad Sci U S A 96:14819-24. 1999
    ..We find that MalE-MinC interacts with FtsZ and prevents polymerization without inhibiting FtsZ's GTPase activity...
  8. ncbi The order of the ring: assembly of Escherichia coli cell division components
    Miguel Vicente
    Centro Nacional de Biotecnologia, Consejo Superior de Investigaciones Cientificas, Campus de Cantoblanco, C Darwin 3, 28049 Madrid, Spain
    Mol Microbiol 61:5-8. 2006
    ..In addition, back-recruitment of all late proteins except FtsN into the division ring occurs even in the absence of proteins incorporated at earlier stages, i.e. FtsA or FtsQ...
  9. pmc ZipA is a MAP-Tau homolog and is essential for structural integrity of the cytokinetic FtsZ ring during bacterial cell division
    D RayChaudhuri
    Department of Molecular Biology and Microbiology, Tufts University School of Medicine, 136 Harrison Avenue, Boston, MA 02111, USA
    EMBO J 18:2372-83. 1999
    The first visible event in prokaryotic cell division is the assembly of the soluble, tubulin-like FtsZ GTPase into a membrane-associated cytokinetic ring that defines the division plane in bacterial and archaeal cells...
  10. pmc ZipA-induced bundling of FtsZ polymers mediated by an interaction between C-terminal domains
    C A Hale
    Department of Molecular Biology and Microbiology, Case Western Reserve University School of Medicine, Cleveland, Ohio 44106 4960, USA
    J Bacteriol 182:5153-66. 2000
    b>FtsZ and ZipA are essential components of the septal ring apparatus, which mediates cell division in Escherichia coli. FtsZ is a cytoplasmic tubulin-like GTPase that forms protofilament-like homopolymers in vitro...
  11. pmc New temperature-sensitive alleles of ftsZ in Escherichia coli
    Stephen G Addinall
    Department of Biology, American University of Beirut, Beirut, Lebanon
    J Bacteriol 187:358-65. 2005
    We isolated five new temperature-sensitive alleles of the essential cell division gene ftsZ in Escherichia coli, using P1-mediated, localized mutagenesis...
  12. ncbi Effects of pH and ionic strength on the assembly and bundling of FtsZ protofilaments: a possible role of electrostatic interactions in the bundling of protofilaments
    Tushar K Beuria
    School of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Powai, Mumbai 400076, India
    Int J Biol Macromol 40:30-9. 2006
    Assembly, bundling and stability of FtsZ protofilaments are important for the formation and functioning of the cytokinetic Z-ring during bacterial division...
  13. ncbi Discovery of novel inhibitors of the ZipA/FtsZ complex by NMR fragment screening coupled with structure-based design
    Desiree H H Tsao
    Structural Biology and Computational Chemistry, Wyeth Research, Cambridge, MA 02140, USA
    Bioorg Med Chem 14:7953-61. 2006
    ZipA is a membrane anchored protein in Escherichia coli that interacts with FtsZ, a homolog of eukaryotic tubulins, forming a septal ring structure that mediates bacterial cell division...
  14. ncbi FtsZ polymer-bundling by the Escherichia coli ZapA orthologue, YgfE, involves a conformational change in bound GTP
    Elaine Small
    Faculty of Life Sciences, University of Manchester, Michael Smith Building, Oxford Road, Manchester, M13 9PT, UK
    J Mol Biol 369:210-21. 2007
    ..driven by a dynamic, ring-shaped, cytoskeletal element (the Z-ring) made up of polymers of the tubulin-like protein FtsZ. It is thought that lateral associations between FtsZ polymers are important for function of the Z-ring in vivo, ..
  15. ncbi Escherichia coli cell-division gene ftsZ encodes a novel GTP-binding protein
    D RayChaudhuri
    Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, Massachusetts 02111
    Nature 359:251-4. 1992
    ..coli cell division, ftsZ plays a central role at the earliest known step of septation...
  16. pmc Cytokinesis in bacteria
    Jeffery Errington
    Sir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, United Kingdom
    Microbiol Mol Biol Rev 67:52-65, table of contents. 2003
    ..b>FtsZ is a cytosolic tubulin-like protein that polymerizes into an oligomeric structure that forms the initial ring at ..
  17. ncbi Bacterial cell division and the septal ring
    David S Weiss
    Department of Microbiology, University of Iowa, Iowa City, IA 52242, USA
    Mol Microbiol 54:588-97. 2004
    ..The foundation of the septal ring is a polymer of the tubulin-like protein FtsZ. Recently, experiments using fluorescence recovery after photobleaching have revealed that the Z ring is extremely ..
  18. ncbi Maturation of the Escherichia coli divisome occurs in two steps
    Mirjam E G Aarsman
    Molecular Cytology, Swammerdam Institute for Life Sciences, University of Amsterdam, Kruislaan 316, 1098 SM Amsterdam, The Netherlands
    Mol Microbiol 55:1631-45. 2005
    Cell division proteins FtsZ (FtsA, ZipA, ZapA), FtsE/X, FtsK, FtsQ, FtsL/B, FtsW, PBP3, FtsN and AmiC localize at mid cell in Escherichia coli in an interdependent order as listed...
  19. ncbi Activation of the Escherichia coli cell division protein FtsZ by a low-affinity interaction with monovalent cations
    Michael Tadros
    Centro Nacional de Biotecnologia, Consejo Superior de Investigaciones Cientificas, Madrid, Spain
    FEBS Lett 580:4941-6. 2006
    We have investigated the activation of FtsZ by monovalent cations. FtsZ polymerization was dependent on the concentrations of protein and monovalent salts, and was accompanied by the uptake of a single ion per monomer added...
  20. pmc The ftsA* gain-of-function allele of Escherichia coli and its effects on the stability and dynamics of the Z ring
    Brett Geissler
    Department of Microbiology and Molecular Genetics, University of Texas Medical School, 6431 Fannin Street, Houston, TX 77030, USA
    Microbiology 153:814-25. 2007
    Formation of the FtsZ ring (Z ring) in Escherichia coli is the first step in the assembly of the divisome, a protein machine required for cell division...
  21. pmc Energetics and geometry of FtsZ polymers: nucleated self-assembly of single protofilaments
    Sonia Huecas
    Centro de Investigaciones Biologicas, Madrid, Spain
    Biophys J 94:1796-806. 2008
    Essential cell division protein FtsZ is an assembling GTPase which directs the cytokinetic ring formation in dividing bacterial cells...
  22. doi Morphogenesis of rod-shaped sacculi
    Tanneke Den Blaauwen
    Molecular Cytology, Swammerdam Institute for Life Sciences, University of Amsterdam, Amsterdam, The Netherlands
    FEMS Microbiol Rev 32:321-44. 2008
    ..Although this multifunctionality and flexibility form a barrier to the functional elucidation of its individual subunits, it helps the cells to survive a variety of emergency situations and to proliferate securely...
  23. doi Novel coiled-coil cell division factor ZapB stimulates Z ring assembly and cell division
    Gitte Ebersbach
    Department of Biochemistry and Molecular Biology, University of Southern Denmark, Campusvej 55, DK 5230 Odense M, Denmark
    Mol Microbiol 68:720-35. 2008
    ..Localization of ZapB to the divisome depended on FtsZ but not FtsA, ZipA or FtsI, and ZapB interacted with FtsZ in a bacterial two-hybrid analysis...
  24. pmc Force generation by a dynamic Z-ring in Escherichia coli cell division
    Jun F Allard
    Department of Mathematics, University of British Columbia, Vancouver, BC, Canada V6T 1Z2
    Proc Natl Acad Sci U S A 106:145-50. 2009
    b>FtsZ, a bacterial homologue of tubulin, plays a central role in bacterial cell division...
  25. pmc The GTPase activity of Escherichia coli FtsZ determines the magnitude of the FtsZ polymer bundling by ZapA in vitro
    Tamimount Mohammadi
    Swammerdam Institute for Life Sciences, Molecular Cytology, Science Park 904, 1098 XH Amsterdam, The Netherlands
    Biochemistry 48:11056-66. 2009
    b>FtsZ polymerizes in a ring-like structure at mid cell to initiate cell division in Escherichia coli. The ring is stabilized by a number of proteins among which the widely conserved ZapA protein...
  26. pmc The proper ratio of FtsZ to FtsA is required for cell division to occur in Escherichia coli
    K Dai
    Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City 66103
    J Bacteriol 174:6145-51. 1992
    ..in Escherichia coli were investigated by examining the effect on cell division of increasing the expression of the ftsZ, ftsA, or ftsQ genes...
  27. pmc Isolation and characterization of ftsZ alleles that affect septal morphology
    E Bi
    Department of Microbiology, Molecular Genetics, and Immunology, University of Kansas Medical Center, Kansas City 66103
    J Bacteriol 174:5414-23. 1992
    The ftsZ gene encodes an essential cell division protein that specifically localizes to the septum of dividing cells. In this study we characterized the effects of the ftsZ2(Rsa) mutation on cell physiology...
  28. pmc A factor that positively regulates cell division by activating transcription of the major cluster of essential cell division genes of Escherichia coli
    X D Wang
    Department of Microbiology, University of Connecticut Health Center, Farmington 06032
    EMBO J 10:3363-72. 1991
    Cell division in Escherichia coli requires the products of the ftsQ, ftsA and ftsZ genes. It is not known how the cell regulates the cellular concentrations of these essential elements of the division system...
  29. ncbi FtsZ ring structure associated with division in Escherichia coli
    E F Bi
    Department of Microbiology, University of Kansas Medical Center, Kansas City 66103
    Nature 354:161-4. 1991
    ..Of these genes, ftsZ is of great interest as it acts earliest in the division pathway, is essential, its level dictates the frequency of ..
  30. pmc ftsZ is an essential cell division gene in Escherichia coli
    K Dai
    Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City 66103
    J Bacteriol 173:3500-6. 1991
    The ftsZ gene is thought to be an essential cell division gene in Escherichia coli. We constructed a null allele of ftsZ in a strain carrying additional copies of ftsZ on a plasmid with a temperature-sensitive replication defect...
  31. pmc GTP-dependent polymerization of Escherichia coli FtsZ protein to form tubules
    D Bramhill
    Department of Enzymology, Merck Research Laboratories, Rahway, NJ 07065 0900
    Proc Natl Acad Sci U S A 91:5813-7. 1994
    The FtsZ protein is a GTPase that is essential for cell division in Escherichia coli. During cytokinesis, FtsZ localizes to a ring at the leading edge of septum synthesis...
  32. pmc Guanine nucleotide-dependent assembly of FtsZ into filaments
    A Mukherjee
    Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City 66103
    J Bacteriol 176:2754-8. 1994
    b>FtsZ is an essential cell division protein that is localized to the leading edge of the bacterial septum in a cytokinetic ring. It contains the tubulin signature motif and is a GTP binding protein with a GTPase activity...
  33. pmc Cell division inhibitors SulA and MinCD prevent formation of the FtsZ ring
    E Bi
    Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City 66103
    J Bacteriol 175:1118-25. 1993
    Immunoelectron microscopy was used to assess the effects of inhibitors of cell division on formation of the FtsZ ring in Escherichia coli...
  34. pmc Colocalization of cell division proteins FtsZ and FtsA to cytoskeletal structures in living Escherichia coli cells by using green fluorescent protein
    X Ma
    Department of Microbiology and Molecular Genetics, University of Texas Medical School, Houston 77030, USA
    Proc Natl Acad Sci U S A 93:12998-3003. 1996
    In the current model for bacterial cell division, FtsZ protein forms a ring that marks the division plane, creating a cytoskeletal framework for the subsequent action of other proteins such as FtsA...
  35. ncbi Contribution of individual promoters in the ddlB-ftsZ region to the transcription of the essential cell-division gene ftsZ in Escherichia coli
    K Flärdh
    Departamento de Biologia Celular y del Desarrollo, Centro de Investigaciones Biologicas, Consejo Superior de Investigaciones Cientificas, Velazquez, Madrid, Spain
    Mol Microbiol 24:927-36. 1997
    The essential cell-division gene ftsZ is transcribed in Escherichia coli from at least six promoters found within the coding regions of the upstream ddlB, ftsQ, and ftsA genes...
  36. ncbi The MinE ring: an FtsZ-independent cell structure required for selection of the correct division site in E. coli
    D M Raskin
    Department of Molecular Biology and Microbiology, Case Western Reserve University School of Medicine, Cleveland, Ohio 44106 4960, USA
    Cell 91:685-94. 1997
    E. coli cell division is mediated by the FtsZ ring and associated factors...
  37. pmc Dynamic assembly of FtsZ regulated by GTP hydrolysis
    A Mukherjee
    Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City 66160, USA
    EMBO J 17:462-9. 1998
    b>FtsZ forms a cytokinetic ring, designated the Z ring, that directs cytokinesis in prokaryotes...
  38. pmc Roles of FtsA and FtsZ in activation of division sites
    K Begg
    Institute of Cell and Molecular Biology, University of Edinburgh, Scotland
    J Bacteriol 180:881-4. 1998
    Increasing FtsZ induces the formation of minicells at cell poles but does not increase the frequency or timing of central divisions...
  39. pmc Bacterial SOS checkpoint protein SulA inhibits polymerization of purified FtsZ cell division protein
    D Trusca
    Department of Enzymology, Merck Research Laboratories, Rahway, New Jersey 07065 0900, USA
    J Bacteriol 180:3946-53. 1998
    ..The SulA protein interacts with the tubulin-like FtsZ division protein. We investigated the effects of purified SulA upon FtsZ...
  40. ncbi Assembly of the FtsZ ring at the central division site in the absence of the chromosome
    Q Sun
    Department of Microbiology and Molecular Genetics, University of Texas Medical School, Houston 77030, USA
    Mol Microbiol 29:491-503. 1998
    The FtsZ ring assembles between segregated daughter chromosomes in prokaryotic cells and is essential for cell division...
  41. pmc Localization of FtsI (PBP3) to the septal ring requires its membrane anchor, the Z ring, FtsA, FtsQ, and FtsL
    D S Weiss
    Department of Microbiology, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Bacteriol 181:508-20. 1999
    ..Septal localization depended upon every other division protein tested (FtsZ, FtsA, FtsQ, and FtsL)...
  42. ncbi FtsZ ring clusters in min and partition mutants: role of both the Min system and the nucleoid in regulating FtsZ ring localization
    X C Yu
    Department of Microbiology and Molecular Genetics, University of Texas Medical School, 6431 Fannin, Houston, TX 77030, USA
    Mol Microbiol 32:315-26. 1999
    ..To understand further the role of the nucleoid and the min system in selection of the cell division site, we examined FtsZ localization in Escherichia coli cells lacking MinCDE and in parC mutants defective in chromosome segregation...
  43. pmc Analysis of MinC reveals two independent domains involved in interaction with MinD and FtsZ
    Z Hu
    Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City 66160, USA
    J Bacteriol 182:3965-71. 2000
    In Escherichia coli FtsZ assembles into a Z ring at midcell while assembly at polar sites is prevented by the min system...
  44. pmc The bacterial cell-division protein ZipA and its interaction with an FtsZ fragment revealed by X-ray crystallography
    L Mosyak
    Biological Chemistry, Wyeth Research, 87 Cambridge Park Drive, Cambridge, MA 02140, USA
    EMBO J 19:3179-91. 2000
    In Escherichia coli, FtsZ, a homologue of eukaryotic tubulins, and ZipA, a membrane-anchored protein that binds to FtsZ, are two essential components of the septal ring structure that mediates cell division...
  45. pmc Influence of the nucleoid on placement of FtsZ and MinE rings in Escherichia coli
    Q Sun
    Department of Microbiology and Molecular Genetics, University of Texas Houston Medical School, Houston, Texas 77030, USA
    J Bacteriol 183:1413-22. 2001
    ..but unsegregated nucleoids, along with the Min system, act as topological inhibitors to restrict assembly of the FtsZ ring (Z ring) to discrete sites in the cell...
  46. ncbi The tubulin ancestor, FtsZ, draughtsman, designer and driving force for bacterial cytokinesis
    Stephen G Addinall
    School of Biological Sciences, University Manchester, 2 205 Stopford Building, Oxford Road, Manchester M13 9PT, UK
    J Mol Biol 318:219-36. 2002
    We discuss in this review the regulation of synthesis and action of FtsZ, its structure in relation to tubulin and microtubules, and the mechanism of polymerization and disassembly (contraction) of FtsZ rings from a specific nucleation ..
  47. pmc Growth rate-dependent regulation of medial FtsZ ring formation
    Richard B Weart
    Department of Biology, Washington University, St Louis, Missouri 63130, USA
    J Bacteriol 185:2826-34. 2003
    b>FtsZ is an essential cell division protein conserved throughout the bacteria and archaea. In response to an unknown cell cycle signal, FtsZ polymerizes into a ring that establishes the future division site...
  48. pmc Concentration and assembly of the division ring proteins FtsZ, FtsA, and ZipA during the Escherichia coli cell cycle
    Sonsoles Rueda
    Centro Nacional de Biotecnologia, Consejo Superior de Investigaciones Cientificas, Campus de Cantoblanco, 28049 Madrid, Spain
    J Bacteriol 185:3344-51. 2003
    The concentration of the cell division proteins FtsZ, FtsA, and ZipA and their assembly into a division ring during the Escherichia coli B/r K cell cycle have been measured in synchronous cultures obtained by the membrane elution ..
  49. ncbi Essential cell division protein FtsZ assembles into one monomer-thick ribbons under conditions resembling the crowded intracellular environment
    Jose Manuel Gonzalez
    Centro de Investigaciones Biologicas, Universidad Autonoma, Madrid, Spain
    J Biol Chem 278:37664-71. 2003
    ..bacterial cytoplasmic environment have been used to study the assembly of the essential cell division protein FtsZ from Escherichia coli...
  50. pmc In vivo characterization of Escherichia coli ftsZ mutants: effects on Z-ring structure and function
    Jesse Stricker
    Department of Cell Biology, Duke University Medical Center, Durham, North Carolina, USA
    J Bacteriol 185:4796-805. 2003
    We have characterized the in vivo phenotypes of 17 mutations of Escherichia coli ftsZ. In particular, we determined whether these mutations can complement a null ftsZ phenotype, and we demonstrated that two noncomplementing mutations show ..
  51. ncbi Development of a fluorescence polarization assay to screen for inhibitors of the FtsZ/ZipA interaction
    Cynthia Hess Kenny
    Screening Sciences, Biophysics Enzymology, Wyeth Research, Pearl River, NY 10965, USA
    Anal Biochem 323:224-33. 2003
    A fluorescence polarization competition assay has been developed to screen for inhibitors of the Escherichia coli FtsZ/ZipA protein-protein interaction...
  52. ncbi Rate-limiting guanosine 5'-triphosphate hydrolysis during nucleotide turnover by FtsZ, a prokaryotic tubulin homologue involved in bacterial cell division
    Laura Romberg
    Department of Cell Biology, Harvard Medical School, 250 Longwood Avenue, Boston, MA 02115, USA
    Biochemistry 43:282-8. 2004
    b>FtsZ is a prokaryotic tubulin homologue that polymerizes into a dynamic ring during cell division. GTP binding and hydrolysis provide the energy for FtsZ dynamics...
  53. ncbi R174 of Escherichia coli FtsZ is involved in membrane interaction and protofilament bundling, and is essential for cell division
    Cecile Marie Koppelman
    Swammerdam Institute for Life Sciences, BioCentrum Amsterdam, Molecular Cytology, University of Amsterdam, Kruislaan 316, 1098 SM Amsterdam, The Netherlands
    Mol Microbiol 51:645-57. 2004
    We investigated the interaction between FtsZ and the cytoplasmic membrane using inside-out vesicles...
  54. ncbi Design and synthesis of indolo[2,3-a]quinolizin-7-one inhibitors of the ZipA-FtsZ interaction
    Lee D Jennings
    Wyeth Research, Department of Medicinal Chemistry, 401N Middletown Rd, Pearl River, NY 10965, USA
    Bioorg Med Chem Lett 14:1427-31. 2004
    The binding of FtsZ to ZipA is a potential target for antibacterial therapy...
  55. ncbi Ruthenium red-induced bundling of bacterial cell division protein, FtsZ
    Manas Kumar Santra
    School of Biosciences and Bioengineering, Indian Institute of Technology, Bombay, Powai, Mumbai 400076, India
    J Biol Chem 279:25959-65. 2004
    The assembly of FtsZ plays a major role in bacterial cell division, and it is thought that the assembly dynamics of FtsZ is a finely regulated process. Here, we show that ruthenium red is able to modulate FtsZ assembly in vitro...
  56. pmc Assembly dynamics of FtsZ rings in Bacillus subtilis and Escherichia coli and effects of FtsZ-regulating proteins
    David E Anderson
    Department of Cell Biology, Duke University Medical Center, Durham, North Carolina 27710, USA
    J Bacteriol 186:5775-81. 2004
    b>FtsZ is the major cytoskeletal component of the bacterial cell division machinery. It forms a ring-shaped structure (the Z ring) that constricts as the bacterium divides...
  57. ncbi Combinatorial synthesis of substituted 3-(2-indolyl)piperidines and 2-phenyl indoles as inhibitors of ZipA-FtsZ interaction
    Lee D Jennings
    Wyeth Research, Department of Medicinal Chemistry, 401 N Middletown Road, Pearl River, NY 10965, USA
    Bioorg Med Chem 12:5115-31. 2004
    The ZipA-FtsZ protein-protein interaction is a potential target for antibacterial therapy. The design and parallel synthesis of a combinatorial library of small molecules, which target the FtsZ binding area on ZipA are described...
  58. pmc FtsZ collaborates with penicillin binding proteins to generate bacterial cell shape in Escherichia coli
    Archana Varma
    Department of Microbiology and Immunology, University of North Dakota School of Medicine and Health Sciences, Grand Forks, ND 58202, USA
    J Bacteriol 186:6768-74. 2004
    ..certain ftsZ84 strains lyse at the nonpermissive temperature instead of filamenting, and inhibition of wild-type FtsZ forces some mutants into tightly wound spirillum-like morphologies...
  59. pmc Cooperative behavior of Escherichia coli cell-division protein FtsZ assembly involves the preferential cyclization of long single-stranded fibrils
    Jose Manuel Gonzalez
    Centro de Investigaciones Biológicas and Centro Nacional de Biotecnología, Consejo Superior de Investigaciones Cientificas, 28040 Madrid, Spain
    Proc Natl Acad Sci U S A 102:1895-900. 2005
    ..to explain the previously posed question of how a single-stranded filament of the bacterial cell-division protein FtsZ can assemble in an apparently cooperative manner...
  60. pmc FtsZ from divergent foreign bacteria can function for cell division in Escherichia coli
    Masaki Osawa
    Department Cell Biology, Box 3709, Duke University Medical Center, Durham, NC 27710, USA
    J Bacteriol 188:7132-40. 2006
    ..pulmonis (MpuFtsZ) and Bacillus subtilis (BsFtsZ) are only 46% and 53% identical in amino acid sequence to FtsZ from Escherichia coli (EcFtsZ)...
  61. pmc The C-terminal domain of MinC inhibits assembly of the Z ring in Escherichia coli
    Daisuke Shiomi
    Microbiology and Molecular Genetics, University of Texas Medical School, 6431 Fannin, Houston, TX 77030, USA
    J Bacteriol 189:236-43. 2007
    In Escherichia coli, the Min system, consisting of three proteins, MinC, MinD, and MinE, negatively regulates FtsZ assembly at the cell poles, helping to ensure that the Z ring will assemble only at midcell...
  62. ncbi 4',6-Diamidino-2-phenylindole (DAPI) induces bundling of Escherichia coli FtsZ polymers inhibiting the GTPase activity
    Esteban Nova
    Departamento de Biologia, Facultad de Ciencias, Universidad de Chile, Casilla 653, Santiago, Chile
    Arch Biochem Biophys 465:315-9. 2007
    b>FtsZ (Filamentous temperature sensitivity Z) cell division protein from Escherichia coli binds the fluorescence probe DAPI...
  63. pmc Investigation of regulation of FtsZ assembly by SulA and development of a model for FtsZ polymerization
    Alex Dajkovic
    Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City, KS 66160, USA
    J Bacteriol 190:2513-26. 2008
    In Escherichia coli FtsZ organizes into a cytoskeletal ring structure, the Z ring, which effects cell division...
  64. doi MinC spatially controls bacterial cytokinesis by antagonizing the scaffolding function of FtsZ
    Alex Dajkovic
    Department of Chemical Engineering, Johns Hopkins University, Baltimore, Maryland 21218, USA
    Curr Biol 18:235-44. 2008
    ..The Z ring is composed of FtsZ filaments, but their organization in the Z ring is poorly understood...
  65. pmc Investigating intracellular dynamics of FtsZ cytoskeleton with photoactivation single-molecule tracking
    Lili Niu
    Center for Cell Analysis and Modeling, Department of Genetics and Developmental Biology, University of Connecticut Health Center, Farmington, Connecticut
    Biophys J 95:2009-16. 2008
    ..the technique of photoactivation single-molecule tracking by investigating the mobility dynamics of intracellular FtsZ protein molecules in live Escherichia coli cells...
  66. pmc Reconstitution of contractile FtsZ rings in liposomes
    Masaki Osawa
    Department of Cell Biology, Duke University Medical Center, Durham, NC 27710 3709, USA
    Science 320:792-4. 2008
    b>FtsZ is a tubulin homolog and the major cytoskeletal protein in bacterial cell division. It assembles into the Z ring, which contains FtsZ and a dozen other division proteins, and constricts to divide the cell...
  67. pmc The conserved C-terminal tail of FtsZ is required for the septal localization and division inhibitory activity of MinC(C)/MinD
    Bang Shen
    Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City, KS 66160, USA
    Mol Microbiol 72:410-24. 2009
    ..the septal localization and division inhibitory activity of MinC(C)/MinD requires the conserved C-terminal tail of FtsZ. This tail also mediates interaction with two essential division proteins, ZipA and FtsA, to link FtsZ polymers to ..
  68. pmc FtsZ filament dynamics at steady state: subunit exchange with and without nucleotide hydrolysis
    Yaodong Chen
    Department of Cell Biology, Duke University Medical Center, Durham, North Carolina 27710 3709, USA
    Biochemistry 48:6664-73. 2009
    We have measured three aspects of FtsZ filament dynamics at steady state: rates of GTP hydrolysis, subunit exchange between protofilaments, and disassembly induced by dilution or excess GDP...
  69. pmc ClpXP protease degrades the cytoskeletal protein, FtsZ, and modulates FtsZ polymer dynamics
    Jodi L Camberg
    Laboratory of Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 106:10614-9. 2009
    b>FtsZ is the major cytoskeletal protein in bacteria and a tubulin homologue. It polymerizes and forms a ring where constriction occurs to divide the cell. We found that FtsZ is degraded by E. coli ClpXP, an ATP-dependent protease...
  70. pmc Curved FtsZ protofilaments generate bending forces on liposome membranes
    Masaki Osawa
    Department of Cell Biology, Duke University Medical Center, Durham, NC, USA
    EMBO J 28:3476-84. 2009
    We have created FtsZ-YFP-mts where an amphipathic helix on the C-terminus tethers FtsZ to the membrane. When incorporated inside multi-lamellar tubular liposomes, FtsZ-YFP-mts can assemble Z rings that generate a constriction force...
  71. doi Examination of the interaction between FtsZ and MinCN in E. coli suggests how MinC disrupts Z rings
    Bang Shen
    Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City, KS 66160, USA
    Mol Microbiol 75:1285-98. 2010
    ..The MinC protein has two domains of equal size and both domains can target FtsZ and block cell division in the proper context...
  72. pmc In vivo structure of the E. coli FtsZ-ring revealed by photoactivated localization microscopy (PALM)
    Guo Fu
    Department of Biophysics and Biophysical Chemistry, Johns Hopkins University School of Medicine, Baltimore, Maryland, United States of America
    PLoS ONE 5:e12682. 2010
    The FtsZ protein, a tubulin-like GTPase, plays a pivotal role in prokaryotic cell division. In vivo it localizes to the midcell and assembles into a ring-like structure-the Z-ring...
  73. doi Cross-linking FtsZ polymers into coherent Z rings
    Alex Dajkovic
    Department of Chemical and Biomolecular Engineering, Johns Hopkins Physical Sciences in Oncology Center, Johns Hopkins University, 3400 N Charles St, Baltimore, MD 21218, USA
    Mol Microbiol 78:651-68. 2010
    ..Here we report that a widely conserved FtsZ binding protein, ZapA, has cytological, biochemical and biophysical properties that argue for the importance of ..
  74. pmc Nucleoid occlusion factor SlmA is a DNA-activated FtsZ polymerization antagonist
    Hongbaek Cho
    Department of Microbiology and Molecular Genetics and Division of Infectious Diseases, Children s Hospital, Harvard Medical School, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 108:3773-8. 2011
    The tubulin-like FtsZ protein initiates assembly of the bacterial cytokinetic machinery by polymerizing into a ring structure, the Z ring, at the prospective site of division...
  75. pmc Identification of ZapD as a cell division factor that promotes the assembly of FtsZ in Escherichia coli
    Jorge Durand-Heredia
    Department of Biology, City College of New York, New York, New York, USA
    J Bacteriol 194:3189-98. 2012
    The tubulin homolog FtsZ forms a polymeric membrane-associated ring structure (Z ring) at midcell that establishes the site of division and provides an essential framework for the localization of a multiprotein molecular machine that ..
  76. pmc Disassembly of the divisome in Escherichia coli: evidence that FtsZ dissociates before compartmentalization
    Bill Söderström
    Center for Biomembrane Research, Department of Biochemistry and Biophysics, Stockholm University, SE 106 91, Stockholm, Sweden
    Mol Microbiol 92:1-9. 2014
    ..b>FtsZ is the first of the divisome proteins to accumulate at the division site and is widely thought to function as a ..
  77. ncbi Recruitment of ZipA to the division site by interaction with FtsZ
    Z Liu
    Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City 66160, USA
    Mol Microbiol 31:1853-61. 1999
    ..As it is anchored to the membrane and interacts with FtsZ, it is a candidate for tethering FtsZ filaments to the membrane during the formation of the Z ring...
  78. ncbi Preferential cytoplasmic location of FtsZ, a protein essential for Escherichia coli septation
    J Pla
    Centro de Investigaciones Biologicas, CSIC, Velazquez, Madrid, Spain
    Mol Microbiol 5:1681-6. 1991
    An ftsZ thermonull mutant has been constructed in which the ftsZ gene has been deleted from the Escherichia coli chromosome while maintaining a wild-type copy of the gene in a thermosensitive plasmid...
  79. ncbi Direct binding of FtsZ to ZipA, an essential component of the septal ring structure that mediates cell division in E. coli
    C A Hale
    Department of Molecular Biology and Microbiology, Case Western Reserve University School of Medicine, Cleveland, Ohio 44106 4960, USA
    Cell 88:175-85. 1997
    b>FtsZ is a soluble, tubulin-like GTPase that forms a membrane-associated ring at the division site of bacterial cells...
  80. ncbi Assembly dynamics of the bacterial cell division protein FTSZ: poised at the edge of stability
    Laura Romberg
    Institute for Cellular and Chemical Biology, Harvard Medical School, SGM 604, 250 Longwood Avenue, Boston, Massachusetts 02115, USA
    Annu Rev Microbiol 57:125-54. 2003
    b>FtsZ is a prokaryotic tubulin homolog that assembles into a ring at the future site of cell division. The resulting "Z ring" forms the framework for the division apparatus, and its assembly is regulated throughout the bacterial cell cycle...
  81. pmc FtsZ exhibits rapid movement and oscillation waves in helix-like patterns in Escherichia coli
    Swapna Thanedar
    Department of Microbiology and Molecular Genetics, University of Texas Medical School, 6431 Fannin Street, Houston, TX 77030, USA
    Curr Biol 14:1167-73. 2004
    Prokaryotes contain cytoskeletal proteins such as the tubulin-like FtsZ, which forms the Z ring at the cell center for cytokinesis, and the actin-like MreB, which forms a helix along the long axis of the cell and is required for shape ..
  82. pmc A rapid fluorescence assay for FtsZ assembly indicates cooperative assembly with a dimer nucleus
    Yaodong Chen
    Department of Cell Biology, Duke University Medical Center, Durham, North Carolina, USA
    Biophys J 88:505-14. 2005
    b>FtsZ is the major cytoskeletal protein operating in bacterial cell division. FtsZ assembles into protofilaments in vitro, and there has been some controversy over whether the assembly is isodesmic or cooperative...
  83. ncbi Visualization of single Escherichia coli FtsZ filament dynamics with atomic force microscopy
    Jesus Mingorance
    Centro Nacional de Biotecnologia, Consejo Superior de Investigaciones Científicas and Instituto Nicolás Cabrera, Facultad de Ciencias, Universidad Autonoma de Madrid, Madrid 28049, Spain
    J Biol Chem 280:20909-14. 2005
    b>FtsZ, the prokaryotic homologue of tubulin, is an essential cell division protein. In the cell, it localizes at the center, forming a ring that constricts during division...
  84. pmc Rapid in vitro assembly dynamics and subunit turnover of FtsZ demonstrated by fluorescence resonance energy transfer
    Yaodong Chen
    Department of Cell Biology, Duke University Medical Center, Durham, NC 27710, USA
    J Biol Chem 280:22549-54. 2005
    We have developed an assay for the assembly of FtsZ based on fluorescence resonance energy transfer (FRET)...
  85. pmc SlmA, a nucleoid-associated, FtsZ binding protein required for blocking septal ring assembly over Chromosomes in E. coli
    Thomas G Bernhardt
    Department of Molecular Biology and Microbiology, School of Medicine, Case Western Reserve University, Cleveland, Ohio 44106, USA
    Mol Cell 18:555-64. 2005
    Cell division in Escherichia coli begins with assembly of the tubulin-like FtsZ protein into a ring structure just underneath the cell membrane...
  86. pmc Dancing around the divisome: asymmetric chromosome segregation in Escherichia coli
    Xindan Wang
    Department of Biochemistry, University of Oxford, Oxford OX1 3QU, United Kingdom
    Genes Dev 19:2367-77. 2005
    ..By mid-S phase, FtsZ forms a ring at mid-cell at the time of initiation of nucleoid separation; ter remains polar...
  87. pmc Condensation of FtsZ filaments can drive bacterial cell division
    Ganhui Lan
    Department of Mechanical Engineering, Whitaker Insitute of Biomedical Engineering, The Johns Hopkins University, Baltimore, MD 21218, USA
    Proc Natl Acad Sci U S A 106:121-6. 2009
    ..Instead, a division ring (Z-ring) consists of mostly FtsZ, FtsA, and ZipA is used to exerting a contractile force...
  88. doi Structural and functional model for ionic (K(+)/Na(+)) and pH dependence of GTPase activity and polymerization of FtsZ, the prokaryotic ortholog of tubulin
    Jesús Mendieta
    Centro de Biologia Molecular Severo Ochoa, Madrid, Spain
    J Mol Biol 390:17-25. 2009
    Bacterial cell division occurs through the formation of a protein ring (division ring) at the site of division, with FtsZ being its main component in most bacteria...
  89. pmc Modeling the physics of FtsZ assembly and force generation
    Harold P Erickson
    Department of Cell Biology, Duke University Medical Center, Durham, NC 27710, USA
    Proc Natl Acad Sci U S A 106:9238-43. 2009
    The tubulin homolog FtsZ is the major cytoskeletal protein in bacterial cytokinesis. It can generate a constriction force on the bacterial membrane or inside tubular liposomes...
  90. pmc Conditional lethal amber mutations in essential Escherichia coli genes
    Christopher D Herring
    Laboratory of Genetics, University of Wisconsin Madison, 445 Henry Mall, Madison, WI 53706, USA
    J Bacteriol 186:2673-81. 2004
    ..We were unable to isolate an amber mutation in ftsZ. Kinetics of cell death and morphological changes were measured following removal of arabinose...
  91. pmc FtsZ condensates: an in vitro electron microscopy study
    David Popp
    ERATO Actin Filament Dynamics Project, Japan Science and Technology Corporation, c o RIKEN Harima Institute at Spring 8, 1 1 1 Kouto, Sayo, Hyogo 679 5148, Japan
    Biopolymers 91:340-50. 2009
    In vivo cell division protein FtsZ from E. coli forms rings and spirals which have only been observed by low resolution light microscopy...
  92. ncbi The coupling between ftsZ transcription and initiation of DNA replication is not mediated by the DnaA-boxes upstream of ftsZ or by DnaA
    R W Smith
    Institute of Cell and Molecular Biology, University of Edinburgh, UK
    Mol Microbiol 21:361-72. 1996
    ..the essential cell-division genes, ftsQ and ftsA, have been hypothesized to mediate the response of the downstream ftsZ gene to intracellular levels of DnaA, and thus to couple the processes of initiation and cell division...
  93. ncbi Spatial regulation of cytokinesis in bacteria
    W Margolin
    Department of Microbiology and Molecular Genetics, University of Texas Medical School, 6431 Fannin, Houston, TX 77030, USA
    Curr Opin Microbiol 4:647-52. 2001
    Cytokinesis in bacteria such as Escherichia coli is orchestrated by FtsZ, a tubulin-like protein that forms a circumferential Z ring at the division site...
  94. pmc New insights into the developmental history of the bacterial cell division site
    Lawrence Rothfield
    Department of Microbiology, University of Connecticut Health Center, Farmington, Connecticut 06032, USA
    J Bacteriol 185:1125-7. 2003
  95. pmc Crystal structure of the SOS cell division inhibitor SulA and in complex with FtsZ
    Suzanne C Cordell
    Medical Research Council, Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, United Kingdom
    Proc Natl Acad Sci U S A 100:7889-94. 2003
    SulA halts cell division in Escherichia coli by binding to the major component of the division machinery FtsZ. We have solved the crystal structure of SulA alone and in complex with FtsZ from Pseudomonas aeruginosa...
  96. ncbi SulA-independent filamentation of Escherichia coli during growth after release from high hydrostatic pressure treatment
    T Kawarai
    Department of Food Science and Technology, College of Bioresource Sciences, Nihon University, Kameino 1866, Fujisawa, Kanagawa 252 8510, Japan
    Appl Microbiol Biotechnol 64:255-62. 2004
    ..Nucleoids segregated normally in the filamentous cells. Only one FtsZ ring (or none) was detected at possible division sites in the elongated cells...
  97. pmc Premature targeting of a cell division protein to midcell allows dissection of divisome assembly in Escherichia coli
    Nathan W Goehring
    Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA
    Genes Dev 19:127-37. 2005
    ..We fused FtsQ to the FtsZ-binding protein ZapA such that FtsQ was targeted to FtsZ rings independently of proteins FtsA and FtsK, which are ..
  98. ncbi A deficiency in S-adenosylmethionine synthetase interrupts assembly of the septal ring in Escherichia coli K-12
    Shan Wang
    Biology Department, Concordia University, 1455 de Maisonneuve Ave, Montreal, Quebec H3G 1M8, Canada
    Mol Microbiol 58:791-9. 2005
    ..Examination with GFP fusions to various septal proteins shows that FtsZ, ZipA and FtsA localize to the septal ring, but FtsQ, FtsW, FtsI or FtsN do not...
  99. pmc Noise-induced Min phenotypes in E. coli
    David Fange
    Department of Cell and Molecular Biology, Biomedical Centre, Uppsala University, Uppsala, Sweden
    PLoS Comput Biol 2:e80. 2006
    ..We found that wild-type (wt) and filamentous (ftsZ-) cells are well characterized by the mean-field model, but that a stochastic model is necessary to account for ..
  100. pmc Z-ring force and cell shape during division in rod-like bacteria
    Ganhui Lan
    Department of Mechanical Engineering, The Johns Hopkins University, Baltimore, MD 21218, USA
    Proc Natl Acad Sci U S A 104:16110-5. 2007
    ..Before septum formation, a division ring called the Z-ring, which is made of a filamentous tubulin analog, FtsZ, is seen at the mid cell. Together with several other proteins, FtsZ is essential for cell division...
  101. doi Assembly of the MreB-associated cytoskeletal ring of Escherichia coli
    Purva Vats
    Department of Molecular, Microbial and Structural Biology, University of Connecticut, Health Center, Farmington, CT 06030, USA
    Mol Microbiol 72:170-82. 2009
    ..that MreB redistributes during the cell cycle to form circumferential ring structures that flank the cytokinetic FtsZ ring and appear to be associated with division and segregation of the helical cytoskeleton...

Research Grants3

  1. ASSEMBLY OF THE DIVISION SEPTUM IN ESCHERICHIA COLI
    David Weiss; Fiscal Year: 2003
    ..In this regard, it is worth noting that four of the proteins to be studied here (FtsI and the three transglycosylases) are primary targets of beta-lactam antibiotics. ..
  2. Zeiss LSM510 META confocal-fluorescence spectroscopy
    HAROLD ERICKSON; Fiscal Year: 2003
    ..Projects for this module are proposed by two faculty from Cell Biology and two from Biochemistry. This will be the only FCS facility at Duke University, and we expect additional projects from across the campus. ..
  3. Novel Approaches for the Control of Microbial Pathogens
    JOSEPH F LUTKENHAUS; Fiscal Year: 2010
    ..Finally, the new program has been based on our track record in which eleven investigators supported by the previous COBRE have secured a total of 16 extramural grants. Eight of the eleven have secured 12 NIH grants. ..