ftsW

Summary

Gene Symbol: ftsW
Description: putative lipid II flippase; integral membrane protein; FtsZ ring stabilizer
Alias: ECK0090, JW0087
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. pmc FtsI and FtsW are localized to the septum in Escherichia coli
    L Wang
    Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City, Kansas 66216, USA
    J Bacteriol 180:2810-6. 1998
  2. ncbi Interaction between two murein (peptidoglycan) synthases, PBP3 and PBP1B, in Escherichia coli
    Ute Bertsche
    Mikrobielle Genetik, Universitat Tubingen, D 72076 Tubingen, Auf der Morgenstelle 28, Germany
    Mol Microbiol 61:675-90. 2006
  3. pmc Functional analysis of the cell division protein FtsW of Escherichia coli
    Soumya Pastoret
    Centre d Ingénierie des Protéines, Institut de Chimie, Bât allée de la Chimie, 3, B 4000 Liege, Belgium
    J Bacteriol 186:8370-9. 2004
  4. pmc The Escherichia coli cell division protein FtsW is required to recruit its cognate transpeptidase, FtsI (PBP3), to the division site
    Keri L N Mercer
    Department of Microbiology, University of Iowa, Iowa City, Iowa 52242, USA
    J Bacteriol 184:904-12. 2002
  5. doi The integral membrane FtsW protein and peptidoglycan synthase PBP3 form a subcomplex in Escherichia coli
    Claudine Fraipont
    Centre d Ingénierie des Protéines, Universite de Liege, Institut de Chimie, B6a, B 4000 Sart Tilman, Belgium
    Microbiology 157:251-9. 2011
  6. ncbi ftsW is an essential cell-division gene in Escherichia coli
    D S Boyle
    Institute of Cell and Molecular Biology, University of Edinburgh, UK
    Mol Microbiol 24:1263-73. 1997
  7. pmc Involvement of an essential gene, mviN, in murein synthesis in Escherichia coli
    Azusa Inoue
    Department of Biological Sciences, Graduate School of Science, Tokyo Metropolitan University, Minamiohsawa, Hachioji, Tokyo 192 0397, Japan
    J Bacteriol 190:7298-301. 2008
  8. pmc Bioinformatics identification of MurJ (MviN) as the peptidoglycan lipid II flippase in Escherichia coli
    Natividad Ruiz
    Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA
    Proc Natl Acad Sci U S A 105:15553-7. 2008
  9. pmc Specificity of the transport of lipid II by FtsW in Escherichia coli
    Tamimount Mohammadi
    From Membrane Biochemistry and Biophysics, Department of Chemistry, Faculty of Science, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands
    J Biol Chem 289:14707-18. 2014
  10. doi Direct interactions of early and late assembling division proteins in Escherichia coli cells resolved by FRET
    Svetlana Alexeeva
    Molecular Cytology, Swammerdam Institute for Life Sciences, Faculty of Science, University of Amsterdam, Amsterdam, The Netherlands
    Mol Microbiol 77:384-98. 2010

Research Grants

Scientific Experts

  • David Weiss
  • Jeffery Errington
  • G Karimova
  • H Hara
  • M Vicente
  • Dominique Mengin-Lecreulx
  • M M Khattar
  • Natividad Ruiz
  • Tanneke Den Blaauwen
  • Claudine Fraipont
  • Martine Nguyen-Distèche
  • Tamimount Mohammadi
  • Mirjam E G Aarsman
  • Benoît Wolf
  • Eefjan Breukink
  • Svetlana Alexeeva
  • Andre Piette
  • Beatriz Lara
  • Robert Sijbrandi
  • Ben de Kruijff
  • Jolanda Verheul
  • Soumya Pastoret
  • W D Donachie
  • Silvia Maggi
  • Adeline Derouaux
  • Azusa Inoue
  • V D'Ulisse
  • Roman A Siddiqui
  • Ute Bertsche
  • Nathan W Goehring
  • P Ghelardini
  • M Fagioli
  • Shan Wang
  • L Paolozzi
  • Juan A Ayala
  • G Di Lallo
  • Keri L N Mercer
  • Mandy Lutters
  • Nathaniel I Martin
  • Marlies Diepeveen-de Bruin
  • René van der Ploeg
  • Vincent van Dam
  • Marie Schloesser
  • Ahmed Bouhss
  • André Zapun
  • Thierry Vernet
  • Gertjan S Verhoeven
  • Theodorus W J Gadella
  • Mohammed Terrak
  • Jun ichi Kato
  • Orietta Massidda
  • Luciano Paolozzi
  • Giuseppe Luzi
  • Yoshimitsu Murata
  • Daniela Fadda
  • Shingo Fujisaki
  • Patrizia Ghelardini
  • Naoko Tsuji
  • Hiroshi Takahashi
  • Joachim Volker Höltje
  • Johannes Gumpert
  • Ivonne Heinze
  • Kai Kannenberg
  • Waldemar Vollmer
  • Jon Beckwith
  • Bernhard Schlott
  • Stephan Diekmann
  • Moritz von Rechenberg
  • Matthias Platzer
  • Thomas Kast
  • Mark D Gonzalez
  • Christian Hoischen
  • Otto Holst
  • Frank Grosse
  • L Wang
  • S J Ryan Arends
  • Elaine B Newman
  • Jean van Heijenoort
  • Thessa M F Vinkenvleugel
  • J Lutkenhaus
  • Robert Brasseur
  • Annick Thomas
  • D S Boyle
  • D Barionovi
  • S G Addinall
  • M Ikeda
  • H K Jung
  • M Matsuhashi
  • F Ishino
  • M Wachi

Detail Information

Publications38

  1. pmc FtsI and FtsW are localized to the septum in Escherichia coli
    L Wang
    Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City, Kansas 66216, USA
    J Bacteriol 180:2810-6. 1998
    ..FtsZ displayed limited localization in furazlocillin-treated cells, whereas it was efficiently localized in FtsI-depleted cells. FtsW, another essential cell division protein, was also localized to the septum.
  2. ncbi Interaction between two murein (peptidoglycan) synthases, PBP3 and PBP1B, in Escherichia coli
    Ute Bertsche
    Mikrobielle Genetik, Universitat Tubingen, D 72076 Tubingen, Auf der Morgenstelle 28, Germany
    Mol Microbiol 61:675-90. 2006
    ..Both synthases could be cross-linked in vivo in wild-type cells and in cells lacking FtsW or FtsN. PBP1B localized diffusely and in foci at the septation site and also at the side wall...
  3. pmc Functional analysis of the cell division protein FtsW of Escherichia coli
    Soumya Pastoret
    Centre d Ingénierie des Protéines, Institut de Chimie, Bât allée de la Chimie, 3, B 4000 Liege, Belgium
    J Bacteriol 186:8370-9. 2004
    ..mutagenesis experiments combined with fluorescence microscopy shed light on the role of Escherichia coli FtsW, a membrane protein belonging to the SEDS family that is involved in peptidoglycan assembly during cell elongation, ..
  4. pmc The Escherichia coli cell division protein FtsW is required to recruit its cognate transpeptidase, FtsI (PBP3), to the division site
    Keri L N Mercer
    Department of Microbiology, University of Iowa, Iowa City, Iowa 52242, USA
    J Bacteriol 184:904-12. 2002
    The bacterial cell division protein FtsW has been suggested to perform two functions: stabilize the FtsZ cytokinetic ring, and facilitate septal peptidoglycan synthesis by the transpeptidase FtsI (penicillin-binding protein 3)...
  5. doi The integral membrane FtsW protein and peptidoglycan synthase PBP3 form a subcomplex in Escherichia coli
    Claudine Fraipont
    Centre d Ingénierie des Protéines, Universite de Liege, Institut de Chimie, B6a, B 4000 Sart Tilman, Belgium
    Microbiology 157:251-9. 2011
    ..It requires the putative lipid II flippase FtsW for its localization at the division site and is necessary for the midcell localization of the class A PBP1B...
  6. ncbi ftsW is an essential cell-division gene in Escherichia coli
    D S Boyle
    Institute of Cell and Molecular Biology, University of Edinburgh, UK
    Mol Microbiol 24:1263-73. 1997
    ..complementation of the temperature-sensitive ftsW201 allele requires the presence of the full coding sequence of ftsW plus upstream DNA encompassing the C-terminus of mraY and the full coding sequence of murD...
  7. pmc Involvement of an essential gene, mviN, in murein synthesis in Escherichia coli
    Azusa Inoue
    Department of Biological Sciences, Graduate School of Science, Tokyo Metropolitan University, Minamiohsawa, Hachioji, Tokyo 192 0397, Japan
    J Bacteriol 190:7298-301. 2008
    ..An intermediate in murein synthesis, polyprenyl diphosphate-N-acetylmuramic acid-(pentapeptide)-N-acetyl-glucosamine, accumulated in mutant cells. These results indicated that MviN is involved in murein synthesis...
  8. pmc Bioinformatics identification of MurJ (MviN) as the peptidoglycan lipid II flippase in Escherichia coli
    Natividad Ruiz
    Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA
    Proc Natl Acad Sci U S A 105:15553-7. 2008
    ..Because of its essential nature, MurJ could serve as a target in the continuing search for antimicrobial compounds...
  9. pmc Specificity of the transport of lipid II by FtsW in Escherichia coli
    Tamimount Mohammadi
    From Membrane Biochemistry and Biophysics, Department of Chemistry, Faculty of Science, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands
    J Biol Chem 289:14707-18. 2014
    ..Lipid II, the important building block of the bacterial cell wall, and its inner membrane-localized transporter FtsW. Here, we show that the predicted transmembrane helix 4 of Escherichia coli FtsW (this protein consists of 10 ..
  10. doi Direct interactions of early and late assembling division proteins in Escherichia coli cells resolved by FRET
    Svetlana Alexeeva
    Molecular Cytology, Swammerdam Institute for Life Sciences, Faculty of Science, University of Amsterdam, Amsterdam, The Netherlands
    Mol Microbiol 77:384-98. 2010
    ..find FtsN, one of the latest proteins in the divisome assembly, interacting with late assembling proteins FtsI and FtsW, but also with early (proto-ring) protein ZapA...
  11. pmc Identification of FtsW as a transporter of lipid-linked cell wall precursors across the membrane
    Tamimount Mohammadi
    Department of Chemical Biology and Organic Chemistry, Institute of Biomembranes, Bijvoet Center for Biomolecular Research, Faculty of Science, Utrecht University, Padualaan, Utrecht, The Netherlands
    EMBO J 30:1425-32. 2011
    ..Here, we show that the integral membrane protein FtsW, an essential protein of the bacterial division machinery, is a transporter of the lipid-linked peptidoglycan ..
  12. ncbi The analysis of cell division and cell wall synthesis genes reveals mutationally inactivated ftsQ and mraY in a protoplast-type L-form of Escherichia coli
    Roman A Siddiqui
    Leibniz Institute for Age Research, Jena, Germany
    FEMS Microbiol Lett 258:305-11. 2006
    ..Missense mutations were determined in the ftsA and ftsW products yielding amino-acid replacements at conserved positions...
  13. pmc Structural determinants required to target penicillin-binding protein 3 to the septum of Escherichia coli
    Andre Piette
    Centre d Ingénierie des Protéines, Universite de Liege, Institut de Chimie, B6a, B 4000 Liege, Belgium
    J Bacteriol 186:6110-7. 2004
    ..Based on the effects of increasing production of FtsQ or FtsW on the division of cells expressing PBP3 mutants, it is suggested that these proteins could interact...
  14. doi Division protein interaction web: identification of a phylogenetically conserved common interactome between Streptococcus pneumoniae and Escherichia coli
    Silvia Maggi
    Dipartimento di Biologia, Universita Tor Vergata, Roma, Italy
    Microbiology 154:3042-52. 2008
    ..At least nine division proteins, ZapA, FtsZ, FtsA, FtsK, FtsQ/DivIB, FtsB/DivIC, FtsL, FtsI and FtsW, are believed to have a conserved function between these bacteria and thus we may say that a significant part of ..
  15. pmc The monofunctional glycosyltransferase of Escherichia coli localizes to the cell division site and interacts with penicillin-binding protein 3, FtsW, and FtsN
    Adeline Derouaux
    Centre d Ingénierie des Protéines, Universite de Liege, Institut de Chimie, B6a, B 4000 Sart Tilman Liège, Belgium
    J Bacteriol 190:1831-4. 2008
    ..in PBP1b and produce a thermosensitive PBP1a and is able to interact with three constituents of the divisome, PBP3, FtsW, and FtsN, suggesting that MtgA may play a role in peptidoglycan assembly during the cell cycle in collaboration ..
  16. ncbi Three functional subdomains of the Escherichia coli FtsQ protein are involved in its interaction with the other division proteins
    V D'Ulisse
    Dipartimento di Biologia Università Tor Vergata, Via della Ricerca Scientifica, Roma 00133, Italy
    Microbiology 153:124-38. 2007
    ..coli divisome assembly, is able to interact with various division proteins, namely FtsI, FtsL, FtsN, FtsB and FtsW. In this paper, the FtsQ domains involved in these interactions were identified by two-hybrid assays and co-..
  17. ncbi Premature targeting of cell division proteins to midcell reveals hierarchies of protein interactions involved in divisome assembly
    Nathan W Goehring
    Department of Microbiology and Molecular Genetics, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA
    Mol Microbiol 61:33-45. 2006
    ..Based on these observations we present a revised model for assembly of the E. coli division apparatus...
  18. ncbi The order of the ring: assembly of Escherichia coli cell division components
    Miguel Vicente
    Centro Nacional de Biotecnologia, Consejo Superior de Investigaciones Cientificas, Campus de Cantoblanco, C Darwin 3, 28049 Madrid, Spain
    Mol Microbiol 61:5-8. 2006
    ..In addition, back-recruitment of all late proteins except FtsN into the division ring occurs even in the absence of proteins incorporated at earlier stages, i.e. FtsA or FtsQ...
  19. ncbi A deficiency in S-adenosylmethionine synthetase interrupts assembly of the septal ring in Escherichia coli K-12
    Shan Wang
    Biology Department, Concordia University, 1455 de Maisonneuve Ave, Montreal, Quebec H3G 1M8, Canada
    Mol Microbiol 58:791-9. 2005
    ..with GFP fusions to various septal proteins shows that FtsZ, ZipA and FtsA localize to the septal ring, but FtsQ, FtsW, FtsI or FtsN do not...
  20. ncbi Peptidoglycan precursor pools associated with MraY and FtsW deficiencies or antibiotic treatments
    Beatriz Lara
    Enveloppes Bactériennes et Antibiotiques, UMR 8619 CNRS, Batiment 430, Universite Paris Sud, 91405 Orsay, France
    FEMS Microbiol Lett 250:195-200. 2005
    ..With MraY and FtsW deficiencies the decrease of UDP-MurNAc-pentapeptide was accompanied by an increase of the upstream nucleotide ..
  21. pmc Interaction network among Escherichia coli membrane proteins involved in cell division as revealed by bacterial two-hybrid analysis
    Gouzel Karimova
    Unité de Biochimie des Interactions Macromoléculaires, Institut Pasteur, 75724 Paris, Cedex 15, France
    J Bacteriol 187:2233-43. 2005
    ..In addition, our study shows that the cAMP-based two-hybrid system is particularly appropriate for analyzing molecular interactions between membrane proteins...
  22. ncbi Bacterial cell division and the septal ring
    David S Weiss
    Department of Microbiology, University of Iowa, Iowa City, IA 52242, USA
    Mol Microbiol 54:588-97. 2004
    ..Finally, two murein hydrolases, AmiC and EnvC, have been shown to localize to the septal ring of Escherichia coli, where they play an important role in separation of daughter cells...
  23. ncbi Maturation of the Escherichia coli divisome occurs in two steps
    Mirjam E G Aarsman
    Molecular Cytology, Swammerdam Institute for Life Sciences, University of Amsterdam, Kruislaan 316, 1098 SM Amsterdam, The Netherlands
    Mol Microbiol 55:1631-45. 2005
    Cell division proteins FtsZ (FtsA, ZipA, ZapA), FtsE/X, FtsK, FtsQ, FtsL/B, FtsW, PBP3, FtsN and AmiC localize at mid cell in Escherichia coli in an interdependent order as listed...
  24. ncbi Use of a two-hybrid assay to study the assembly of a complex multicomponent protein machinery: bacterial septosome differentiation
    G Di Lallo
    Dipartimento di Biologia, Universita Tor Vergata, Via della Ricerca Scientifica, I 00133 Rome, Italy
    Microbiology 149:3353-9. 2003
    The ability of each of the nine Escherichia coli division proteins (FtsZ, FtsA, ZipA, FtsK, FtsQ, FtsL, FtsW, FtsI, FtsN) to interact with itself and with each of the remaining eight proteins was studied in 43 possible combinations of ..
  25. pmc Cytokinesis in bacteria
    Jeffery Errington
    Sir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, United Kingdom
    Microbiol Mol Biol Rev 67:52-65, table of contents. 2003
    ..The functions of most of these proteins are unclear with the exception of at least one penicillin-binding protein, which catalyzes a key step in cell wall synthesis in the division septum...
  26. ncbi Topological characterization of the essential Escherichia coli cell division protein FtsW
    Beatriz Lara
    Centro de Biologia Molecular Severo Ochoa, Consejo Superior de Investigaciones Cientificas, Universidad Autonoma de Madrid, Cantoblanco, Madrid, Spain
    FEMS Microbiol Lett 216:23-32. 2002
    The membrane topology of Escherichia coli FtsW, a 46-kDa essential protein, was analyzed using a set of 28 ftsW-alkaline phosphatase (ftsW-phoA) and nine ftsW-beta-lactamase (ftsW-bla) gene fusions obtained by in vivo and in vitro methods...
  27. ncbi Co-ordinate regulation of the Escherichia coli cell cycle or The cloud of unknowing
    W D Donachie
    Department of Molecular Biology, University of Edinburgh, Kings Buildings, Mayfield Road, Edinburgh, UK
    Mol Microbiol 40:779-85. 2001
    ..A discussion of some aspects of the regulation of chromosome replication, segregation and cell division in Escherichia coli...
  28. pmc Contribution of the Pmra promoter to expression of genes in the Escherichia coli mra cluster of cell envelope biosynthesis and cell division genes
    D Mengin-Lecreulx
    Laboratoire des Enveloppes Bactériennes, Centre National de la Recherche Scientifique, Universite Paris Sud, 91405 Orsay Cedex, France
    J Bacteriol 180:4406-12. 1998
    ..cells that lysed in the absence of inducer, a defect which was complemented only when the whole region from Pmra to ftsW, the fifth gene downstream from ftsI, was provided in trans on a plasmid...
  29. ncbi Regulation of transcription of cell division genes in the Escherichia coli dcw cluster
    M Vicente
    Departamento de Biologia Celular y del Desarrollo, Consejo Superior de Investigaciones Cientificas, Madrid, Spain
    Cell Mol Life Sci 54:317-24. 1998
    ..Complementation tests suggest that they extend into ftsW in the central part of the cluster...
  30. pmc A promoter for the first nine genes of the Escherichia coli mra cluster of cell division and cell envelope biosynthesis genes, including ftsI and ftsW
    H Hara
    National Institute of Genetics, Shizuoka ken, Japan
    J Bacteriol 179:5802-11. 1997
    ..The defect was complemented by a plasmid carrying a chromosomal fragment ranging from the mra promoter to ftsW, the fifth gene downstream of ftsI, but not by a plasmid lacking ftsW...
  31. pmc Two polypeptide products of the Escherichia coli cell division gene ftsW and a possible role for FtsW in FtsZ function
    M M Khattar
    Institute of Cell and Molecular Biology, University of Edinburgh, Scotland
    J Bacteriol 179:784-93. 1997
    Two new mutations in the cell division gene ftsW have been isolated and characterized. The ftsW263(Ts) mutation results in a block to division at the initiation stage, similar to that previously observed with the ftsW201(Ts) mutation...
  32. pmc Identification of FtsW and characterization of a new ftsW division mutant of Escherichia coli
    M M Khattar
    Institute of Cell and Molecular Biology, University of Edinburgh, Scotland
    J Bacteriol 176:7140-7. 1994
    The product of the ftsW gene has been identified as a polypeptide that, like the related RodA protein, shows anomalous mobility on sodium dodecyl sulfate-polyacrylamide gel electrophoresis...
  33. pmc Inhibition of growth of ftsQ, ftsA, and ftsZ mutant cells of Escherichia coli by amplification of a chromosomal region encompassing closely aligned cell division and cell growth genes
    H K Jung
    Institute of Applied Microbiology, University of Tokyo, Japan
    J Bacteriol 171:6379-82. 1989
    ..coli strains with mutations of the flanking cell division genes ftsQ, ftsA, and ftsZ. A shortened fragment in which the major portion of ftsI was deleted also had lethal effects on ftsQ and ftsZ mutants...
  34. pmc Structural similarity among Escherichia coli FtsW and RodA proteins and Bacillus subtilis SpoVE protein, which function in cell division, cell elongation, and spore formation, respectively
    M Ikeda
    Institute of Applied Microbiology, University of Tokyo, Japan
    J Bacteriol 171:6375-8. 1989
    The Escherichia coli cell division gene ftsW (2 min) was cloned and sequenced. It encodes a hydrophobic protein(s) with 414 and/or 384 amino acid residues...
  35. pmc Nucleotide sequence involving murG and murC in the mra gene cluster region of Escherichia coli
    M Ikeda
    Institute of Applied Microbiology, University of Tokyo, Japan
    Nucleic Acids Res 18:4014. 1990
  36. pmc Nucleotide sequence involving murD and an open reading frame ORF-Y spacing murF and ftsW in Escherichia coli
    M Ikeda
    Institute of Applied Microbiology, University of Tokyo, Japan
    Nucleic Acids Res 18:1058. 1990
  37. pmc Nucleotide sequence of the cell-envelope murG gene of Escherichia coli
    D Mengin-Lecreulx
    URA 1131 du Centre National de la Recherche Scientifique, Universite Paris Sud, Orsay, France
    Nucleic Acids Res 18:2810. 1990
  38. pmc Nucleotide sequence of the murD gene encoding the UDP-MurNAc-L-Ala-D-Glu synthetase of Escherichia coli
    D Mengin-Lecreulx
    Unité Associée 1131 du Centre National de la Recherche Scientifique, Biochimie Moléculaire et Cellulaire, Universite Paris Sud, Orsay, France
    Nucleic Acids Res 18:183. 1990

Research Grants1

  1. ASSEMBLY OF THE DIVISION SEPTUM IN ESCHERICHIA COLI
    David Weiss; Fiscal Year: 2003
    ..In this regard, it is worth noting that four of the proteins to be studied here (FtsI and the three transglycosylases) are primary targets of beta-lactam antibiotics. ..