fis

Summary

Gene Symbol: fis
Description: global DNA-binding transcriptional dual regulator
Alias: ECK3248, JW3229, nbp
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. ncbi Regulation of ndh expression in Escherichia coli by Fis
    Laura Jackson
    Krebs Institute for Biomolecular Research, Department of Molecular Biology and Biotechnology, University of Sheffield, Western Bank, Sheffield S10 2TN, UK
    Microbiology 150:407-13. 2004
  2. pmc Involvement of Fis protein in replication of the Escherichia coli chromosome
    M Filutowicz
    Department of Bacteriology, University of Wisconsin Madison 53706
    J Bacteriol 174:398-407. 1992
  3. pmc Dramatic changes in Fis levels upon nutrient upshift in Escherichia coli
    C A Ball
    Molecular Biology Institute, University of California, Los Angeles 90024
    J Bacteriol 174:8043-56. 1992
  4. ncbi The Fis protein: it's not just for DNA inversion anymore
    S E Finkel
    Department of Biological Chemistry, UCLA School of Medicine 90024 1737
    Mol Microbiol 6:3257-65. 1992
  5. pmc The E.coli fis promoter is subject to stringent control and autoregulation
    O Ninnemann
    Institut für Genbiologische Forschung Berlin GmbH, Germany
    EMBO J 11:1075-83. 1992
  6. pmc The FIS protein binds and bends the origin of chromosomal DNA replication, oriC, of Escherichia coli
    H Gille
    Max Planck Institut fur Molekulare Genetik, Berlin, FRG
    Nucleic Acids Res 19:4167-72. 1991
  7. pmc E.coli Fis protein activates ribosomal RNA transcription in vitro and in vivo
    W Ross
    Department of Bacteriology, University of Wisconsin, Madison 53706
    EMBO J 9:3733-42. 1990
  8. pmc Escherichia coli host factor for site-specific DNA inversion: cloning and characterization of the fis gene
    C Koch
    Max Planck Institut fur Molekulare Genetik, Otto Warburg Laboratorium, Berlin, Federal Republic of Germany
    Proc Natl Acad Sci U S A 85:4237-41. 1988
  9. pmc FIS and RNA polymerase holoenzyme form a specific nucleoprotein complex at a stable RNA promoter
    G Muskhelishvili
    Institute für Genetik und Mikrobiologie, Munchen, Germany
    EMBO J 14:1446-52. 1995
  10. pmc The Escherichia coli FIS protein is not required for the activation of tyrT transcription on entry into exponential growth
    L R Lazarus
    MRC Laboratory of Molecular Biology, Cambridge, UK
    EMBO J 12:2483-94. 1993

Scientific Experts

  • Remus Th Dame
  • Andrew Travers
  • Jay D Gralla
  • Yukio Asato
  • P P Dennis
  • Hideo Ikeda
  • R G Martin
  • Karin Schnetz
  • A Ishihama
  • Kazuei Igarashi
  • G Gonzalez-Gil
  • Beatriz Galán
  • David C Grainger
  • Douglas F Browning
  • Robert Osuna
  • Yongping Shao
  • Stephen J W Busby
  • R Schneider
  • Leah S Feldman-Cohen
  • Mark Rochman
  • G Muskhelishvili
  • Charles J Dorman
  • Kimberly A Walker
  • Georgi Muskhelishvili
  • Jeffrey A Cole
  • Prabhat Mallik
  • Ingvild Flåtten
  • Reid C Johnson
  • Christina Kahramanoglou
  • Amanda E Rossiter
  • Sylvie Miquel
  • Derrick J P Squire
  • Kelly S Davidge
  • R C Johnson
  • Zeus Saldaña
  • T A Azam
  • Seyyed I Husnain
  • Sonja Hansen
  • Thomas Neusser
  • Imad Baaklini
  • Byung Kwan Cho
  • R L Gourse
  • Ryan K Shultzaberger
  • Tadhg O Cróinín
  • Dalit Weinstein-Fischer
  • Guang Sheng Lei
  • Athanasios Typas
  • Meranda D Bradley
  • Ryosuke L Ohniwa
  • Martijn S Luijsterburg
  • Yi Xin Huo
  • Steven B Zimmerman
  • Timothy S Pratt
  • Thomas D Schneider
  • Ilya G Lyakhov
  • Christine D Hardy
  • Estelle Crozat
  • Dunja Skoko
  • Christine M Beatty
  • Richard L Gourse
  • Alan J Wolfe
  • Xiaofeng F Yang
  • Laura Jackson
  • Wilfredo Colon
  • Jian Zhang
  • Traci B Topping
  • Valorie T Ryan
  • Madoka Yoshida
  • Roisin M Owens
  • Paul N Hengen
  • William A McLaughlin
  • Gad Glaser
  • David A Schneider
  • Dominic Esposito
  • Huijun Zhi
  • Iain K Pemberton
  • Sarah A Hobart
  • Andrew J Pease
  • William Nasser
  • Kirsten Skarstad
  • M M Barker
  • C A Hirvonen
  • Catherine A Wardius
  • Fernando Ruiz-Perez
  • Ian R Henderson
  • Gillian M Fraser
  • Jurgen Zimmermann
  • Sabine Schmidt
  • Rita E Godfrey
  • Vladimir Benes

Detail Information

Publications113 found, 100 shown here

  1. ncbi Regulation of ndh expression in Escherichia coli by Fis
    Laura Jackson
    Krebs Institute for Biomolecular Research, Department of Molecular Biology and Biotechnology, University of Sheffield, Western Bank, Sheffield S10 2TN, UK
    Microbiology 150:407-13. 2004
    ..The histone-like protein Fis binds to three sites [centred at -123 (Fis I), -72, (Fis II) and +51 (Fis III)] in the ndh promoter...
  2. pmc Involvement of Fis protein in replication of the Escherichia coli chromosome
    M Filutowicz
    Department of Bacteriology, University of Wisconsin Madison 53706
    J Bacteriol 174:398-407. 1992
    We report evidence indicating that Fis protein plays a role in initiation of replication at oriC in vivo...
  3. pmc Dramatic changes in Fis levels upon nutrient upshift in Escherichia coli
    C A Ball
    Molecular Biology Institute, University of California, Los Angeles 90024
    J Bacteriol 174:8043-56. 1992
    b>Fis is a small basic DNA-binding protein from Escherichia coli that was identified because of its role in site-specific DNA recombination reactions...
  4. ncbi The Fis protein: it's not just for DNA inversion anymore
    S E Finkel
    Department of Biological Chemistry, UCLA School of Medicine 90024 1737
    Mol Microbiol 6:3257-65. 1992
    ..Examples of this class of proteins are HU, Integration Host Factor, H-NS, and Fis. Fis was originally identified as the factor for inversion stimulation of the homologous Hin and Gin site-specific ..
  5. pmc The E.coli fis promoter is subject to stringent control and autoregulation
    O Ninnemann
    Institut für Genbiologische Forschung Berlin GmbH, Germany
    EMBO J 11:1075-83. 1992
    The DNA binding protein FIS is involved in processes like site specific DNA inversion, lambda excision and stimulation of stable RNA synthesis in Escherichia coli. The amount of FIS protein is subject to dramatic changes during growth...
  6. pmc The FIS protein binds and bends the origin of chromosomal DNA replication, oriC, of Escherichia coli
    H Gille
    Max Planck Institut fur Molekulare Genetik, Berlin, FRG
    Nucleic Acids Res 19:4167-72. 1991
    The FIS protein (factor for inversion stimulation) is known to stimulate site-specific recombination processes, such as the inversion of the G segment of bacteriophage Mu, by binding to specific enhancer sequences...
  7. pmc E.coli Fis protein activates ribosomal RNA transcription in vitro and in vivo
    W Ross
    Department of Bacteriology, University of Wisconsin, Madison 53706
    EMBO J 9:3733-42. 1990
    ..We have used DNase footprinting to define three specific sites in the rrnB P1 UAR that bind Fis, a protein identified previously by its role in recombinational enhancer function in other systems...
  8. pmc Escherichia coli host factor for site-specific DNA inversion: cloning and characterization of the fis gene
    C Koch
    Max Planck Institut fur Molekulare Genetik, Otto Warburg Laboratorium, Berlin, Federal Republic of Germany
    Proc Natl Acad Sci U S A 85:4237-41. 1988
    The Escherichia coli (Es. coli) protein Fis (factor for inversion stimulation) stimulates site-specific DNA inversion of the G segment in phage Mu by binding to a recombinational enhancer...
  9. pmc FIS and RNA polymerase holoenzyme form a specific nucleoprotein complex at a stable RNA promoter
    G Muskhelishvili
    Institute für Genetik und Mikrobiologie, Munchen, Germany
    EMBO J 14:1446-52. 1995
    The Escherichia coli DNA binding protein FIS activates stable RNA promoters during outgrowth of cells from stationary phase...
  10. pmc The Escherichia coli FIS protein is not required for the activation of tyrT transcription on entry into exponential growth
    L R Lazarus
    MRC Laboratory of Molecular Biology, Cambridge, UK
    EMBO J 12:2483-94. 1993
    The Escherichia coli DNA bending protein factor for inversion stimulation (FIS), is neither necessary nor responsible for the stimulation of transcription from the wild type promoter for the tyrT operon (encoding a species of tyrosine ..
  11. pmc The Escherichia coli Fis protein prevents initiation of DNA replication from oriC in vitro
    S Wold
    Department of Biophysics, Institute for Cancer Research, Montebello, Oslo, Norway
    Nucleic Acids Res 24:3527-32. 1996
    b>Fis protein participates in the normal control of chromosomal replication in Escherichia coli. However, the mechanism by which it executes its effect is largely unknown...
  12. pmc Information analysis of Fis binding sites
    P N Hengen
    Laboratory of Mathematical Biology, National Cancer Institute, Frederick Cancer Research and Development Center, PO Box B, Building 469, Room 144, Frederick, MD 21702 1201, USA
    Nucleic Acids Res 25:4994-5002. 1997
    Originally discovered in the bacteriophage Mu DNA inversion system gin, Fis (Factor for Inversion Stimulation) regulates many genetic systems...
  13. ncbi FIS modulates growth phase-dependent topological transitions of DNA in Escherichia coli
    R Schneider
    Institut fur Genetik und Mikrobiologie, LMU Munchen, Germany
    Mol Microbiol 26:519-30. 1997
    The Escherichia coli DNA-binding protein FIS serves as a DNA architectural factor in two unrelated enzymatic reactions, the site-specific inversion of DNA and transcriptional activation of stable RNA promoters...
  14. pmc Growth phase-dependent variation in protein composition of the Escherichia coli nucleoid
    T Ali Azam
    Department of Molecular Genetics, National Institute of Genetics, Mishima, Shizuoka 411 8540, Japan
    J Bacteriol 181:6361-70. 1999
    ..as Rob [right origin binding protein]), DnaA (DNA-binding protein A), Dps (DNA-binding protein from starved cells), Fis (factor for inversion stimulation), Hfq (host factor for phage Q(beta)), H-NS (histone-like nucleoid structuring ..
  15. ncbi Twelve species of the nucleoid-associated protein from Escherichia coli. Sequence recognition specificity and DNA binding affinity
    T A Azam
    Department of Molecular Genetics, National Institute of Genetics, Mishima, Shizuoka 411 8540, Japan
    J Biol Chem 274:33105-13. 1999
    ..replication origin binding protein), DnaA (DNA-binding protein A), Dps (DNA-binding protein from starved cells), Fis (factor for inversion stimulation), Hfq (host factor for phage Q(beta)), H-NS (histone-like nucleoid structuring ..
  16. ncbi A DNA architectural protein couples cellular physiology and DNA topology in Escherichia coli
    R Schneider
    Institut fur Genetik und Mikrobiologie, LMU Munchen, Maria Ward Str 1a, 80638 Munchen, Germany
    Mol Microbiol 34:953-64. 1999
    ..The DNA architectural protein FIS is a regulator of the metabolic reorganization of the cell during early exponential growth phase...
  17. ncbi Escherichia coli response to hydrogen peroxide: a role for DNA supercoiling, topoisomerase I and Fis
    D Weinstein-Fischer
    Department of Molecular Genetics and Biotechnology, The Hebrew University Hadassah Medical School, 91120 Jerusalem, Israel
    Mol Microbiol 35:1413-20. 2000
    ..The peroxide-dependent topA P1 activation is independent of oxyR, but is mediated by Fis. This nucleoid-associated protein binds to the promoter region of topA...
  18. ncbi Two types of localization of the DNA-binding proteins within the Escherichia coli nucleoid
    T A Azam
    Department of Molecular Genetics, National Institute of Genetics, Mishima, Shizuoka 411 8540, Japan
    Genes Cells 5:613-26. 2000
    ..The intracellular localization of these proteins in E. coli could be predicted from these data, but no attempt has been made thus far to directly observe the intracellular distribution of the DNA-binding proteins...
  19. ncbi DNA supercoiling and transcription in Escherichia coli: The FIS connection
    A Travers
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK
    Biochimie 83:213-7. 2001
    The nucleoid-associated protein FIS modulates the topology of DNA in a growth-phase dependent manner functioning homeostatically to counteract excessive levels of negative superhelicity...
  20. pmc An architectural role of the Escherichia coli chromatin protein FIS in organising DNA
    R Schneider
    Institut fuer Genetik und Mikrobiologie, LMU Muenchen, Maria Ward Strasse 1a, D 80638 Muenchen, Germany
    Nucleic Acids Res 29:5107-14. 2001
    The Escherichia coli chromatin protein FIS modulates the topology of DNA in a growth phase-dependent manner. In this study we have investigated the global effect of FIS binding on DNA architecture in vitro...
  21. ncbi FIS modulates the kinetics of successive interactions of RNA polymerase with the core and upstream regions of the tyrT promoter
    Iain K Pemberton
    Enzymologie et cinétique structurale, UMR 8532 du CNRS, LBPA, Ecole Normale Superieure de Cachan, 94235 Cachan, France
    J Mol Biol 318:651-63. 2002
    We have applied laser UV photo-footprinting to characterise kinetically complexes involving the activator protein FIS, RNA polymerase and the tyrT promoter of Escherichia coli...
  22. ncbi Modulation of CRP-dependent transcription at the Escherichia coli acsP2 promoter by nucleoprotein complexes: anti-activation by the nucleoid proteins FIS and IHF
    Douglas F Browning
    Department of Microbiology and Immunology, Stritch School of Medicine, Loyola University Chicago, Maywood, IL 60153, USA
    Mol Microbiol 51:241-54. 2004
    ..used electrophoretic mobility shift assays and DNase I footprint analyses to demonstrate that the nucleoid proteins FIS and IHF each bind multiple sites within the acs regulatory region, that FIS competes successfully with CRP for ..
  23. ncbi Escherichia coli prereplication complex assembly is regulated by dynamic interplay among Fis, IHF and DnaA
    Valorie T Ryan
    Department of Biological Sciences, Florida Institute of Technology, Melbourne, Florida 32901 6795, USA
    Mol Microbiol 51:1347-59. 2004
    Initiator DnaA and DNA bending proteins, Fis and IHF, comprise prereplication complexes (pre-RC) that unwind the Escherichia coli chromosome's origin of replication, oriC...
  24. ncbi Transcription activation by remodelling of a nucleoprotein assembly: the role of NarL at the FNR-dependent Escherichia coli nir promoter
    Douglas F Browning
    School of Biosciences, University of Birmingham, Birmingham B15 2TT, UK
    Mol Microbiol 53:203-15. 2004
    ..but that FNR-dependent activation is repressed by IHF binding to a site centred at position -88 (IHF I) and Fis binding to sites centred at positions -142 (Fis I) and +23 (Fis II)...
  25. ncbi The role of nucleoid-associated proteins in the organization and compaction of bacterial chromatin
    Remus T Dame
    Physics of Complex Systems, Department of Physics and Astronomy, Faculty of Sciences, Vrije Universiteit, Amsterdam, The Netherlands
    Mol Microbiol 56:858-70. 2005
    ..Many of these new insights can be attributed to the use of recently developed biophysical techniques...
  26. ncbi Integration of three signals at the Escherichia coli nrf promoter: a role for Fis protein in catabolite repression
    Douglas F Browning
    School of Biosciences, The University of Birmingham, Birmingham B15 2TT, UK
    Mol Microbiol 57:496-510. 2005
    ..FNR, and by the nitrate/nitrite ion-controlled response regulators, NarL or NarP, but is repressed by the IHF and Fis proteins...
  27. ncbi A genetic selection for supercoiling mutants of Escherichia coli reveals proteins implicated in chromosome structure
    Christine D Hardy
    Department of Molecular and Cell Biology, 16 Barker Hall, University of California, Berkeley, CA 94720 3204, USA
    Mol Microbiol 57:1636-52. 2005
    ..Three of these proteins: H-NS, Fis and DksA, have clear ties to chromosome biology...
  28. ncbi Low-force DNA condensation and discontinuous high-force decondensation reveal a loop-stabilizing function of the protein Fis
    Dunja Skoko
    Department of Physics, University of Illinois at Chicago, 845 West Taylor Street, Chicago, Illinois 60607 7059, USA
    Phys Rev Lett 95:208101. 2005
    We report single-DNA-stretching experiments showing that the protein Fis, an abundant bacterial chromosome protein of E. coli, mediates a dramatic DNA condensation to zero length...
  29. ncbi Cooperative transitions of isolated Escherichia coli nucleoids: implications for the nucleoid as a cellular phase
    Steven B Zimmerman
    Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892 0560, USA
    J Struct Biol 153:160-75. 2006
    ..nor was it changed in nucleoids isolated from cells after exposure to chloramphenicol or from cells in which Dps, Fis, or H-NS and StpA had been deleted...
  30. pmc DksA is required for growth phase-dependent regulation, growth rate-dependent control, and stringent control of fis expression in Escherichia coli
    Prabhat Mallik
    Department of Biological Sciences, University at Albany, 1400 Washington Avenue, Albany, NY 12222, USA
    J Bacteriol 188:5775-82. 2006
    ..Given the kinetic similarities between rRNA promoters and the fis promoter (Pfis), we investigated the possibility that DksA might also control transcription from Pfis...
  31. pmc Association of nucleoid proteins with coding and non-coding segments of the Escherichia coli genome
    David C Grainger
    School of Biosciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK
    Nucleic Acids Res 34:4642-52. 2006
    ..exploited chromatin immunoprecipitation and high-density microarrays to study the binding of three such proteins, FIS, H-NS and IHF, across the E.coli genome in vivo...
  32. pmc Dynamic state of DNA topology is essential for genome condensation in bacteria
    Ryosuke L Ohniwa
    Laboratory of Plasma Membrane and Nuclear Signaling, Kyoto University Graduate School of Biostudies, Kitashirakawa Oiwake cho, Sakyo ku, Kyoto, Japan
    EMBO J 25:5591-602. 2006
    ..that the expression of Dps and the nucleoid condensation was not simply correlated in Escherichia coli, and that Fis, which is an E...
  33. ncbi Effects of Fis on Escherichia coli gene expression during different growth stages
    Meranda D Bradley
    Department of Biological Science, University at Albany, 1400 Washington Avenue, Albany, NY 12222, USA
    Microbiology 153:2922-40. 2007
    b>Fis is a nucleoid-associated protein in Escherichia coli that is abundant during early exponential growth in rich medium but is in short supply during stationary phase...
  34. pmc Functional characterization of the Escherichia coli Fis-DNA binding sequence
    Yongping Shao
    Department of Biological Sciences, University at Albany, 1400 Washington Avenue, Albany, NY 12222, USA
    J Mol Biol 376:771-85. 2008
    The Escherichia coli protein Fis is remarkable for its ability to interact specifically with DNA sites of highly variable sequences. The mechanism of this sequence-flexible DNA recognition is not well understood...
  35. pmc Genome-wide analysis of Fis binding in Escherichia coli indicates a causative role for A-/AT-tracts
    Byung Kwan Cho
    Department of Bioengineering, University of California San Diego, La Jolla, California 92093 0412, USA
    Genome Res 18:900-10. 2008
    We determined the genome-wide distribution of the nucleoid-associated protein Fis in Escherichia coli using chromatin immunoprecipitation coupled with high-resolution whole genome-tiling microarrays...
  36. doi Selective repression by Fis and H-NS at the Escherichia coli dps promoter
    David C Grainger
    School of Biosciences, University of Birmingham, Edgbaston, Birmingham, UK
    Mol Microbiol 68:1366-77. 2008
    ..Here we show that two other nucleoid-associated proteins, Fis and H-NS, can bind at the dps gene promoter and downregulate its activity...
  37. pmc Downregulation of the Escherichia coli guaB promoter by FIS
    Seyyed I Husnain
    F Floor, School of Medicine and Biomedical Sciences, University of Sheffield, Beech Hill Road, Sheffield S10 2RX, UK
    Microbiology 154:1729-38. 2008
    ..Here we investigated the role of the factor for inversion stimulation (FIS) in the regulation of this promoter...
  38. doi The role of FIS protein in the physiological control of the expression of the Escherichia coli meta-hpa operon
    Beatriz Galán
    Department of Molecular Microbiology, Centro de Investigaciones Biologicas, CSIC, Madrid, Spain
    Microbiology 154:2151-60. 2008
    ..We present in vitro and in vivo studies to demonstrate the involvement of FIS protein in this catabolic repression...
  39. pmc Direct and indirect effects of H-NS and Fis on global gene expression control in Escherichia coli
    Christina Kahramanoglou
    Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QP, UK
    Nucleic Acids Res 39:2073-91. 2011
    ..Two of these--H-NS and Fis--bind to specific DNA sequences and structures...
  40. pmc The Fis protein has a stimulating role in initiation of replication in Escherichia coli in vivo
    Ingvild Flåtten
    Department of Cell Biology, Institute for Cancer Research, The Norwegian Radiumhospital, Oslo University Hospital, Oslo, Norway
    PLoS ONE 8:e83562. 2013
    The Fis protein is a nucleoid associated protein that has previously been reported to act negatively in initiation of replication in Escherichia coli...
  41. pmc Identification of two functional regions in Fis: the N-terminus is required to promote Hin-mediated DNA inversion but not lambda excision
    R Osuna
    Department of Biological Chemistry, School of Medicine, University of California, Los Angeles 90024
    EMBO J 10:1593-603. 1991
    The Fis protein of E. coli binds to a recombinational enhancer sequence that is required to stimulate Hin-mediated DNA inversion. Fis is also required for efficient lambda prophase excision in vivo...
  42. pmc The molecular structure of wild-type and a mutant Fis protein: relationship between mutational changes and recombinational enhancer function or DNA binding
    H S Yuan
    Molecular Biology Institute, University of California, Los Angeles 90024
    Proc Natl Acad Sci U S A 88:9558-62. 1991
    The 98-amino acid Fis protein from Escherichia coli functions in a variety of reactions, including promotion of Hin-mediated site-specific DNA inversion when bound to an enhancer sequence...
  43. ncbi FIS-dependent trans-activation of tRNA and rRNA operons of Escherichia coli
    L Bosch
    Department of Biochemistry, Leiden University, Gorlaeus Laboratories, The Netherlands
    Biochim Biophys Acta 1050:293-301. 1990
    ..Both in vivo and in vitro experiments show that the Escherichia coli protein FIS (Factor for Inversion Stimulation) is involved in the trans-activation...
  44. ncbi The ndh-binding protein (Nbp) regulates the ndh gene of Escherichia coli in response to growth phase and is identical to Fis
    J Green
    Krebs Institute, Department of Molecular Biology and Biotechnology, University of Sheffield, UK
    Mol Microbiol 20:1043-55. 1996
    ..Protein purification and mutant studies have further shown that Nbp is identical to the Fis protein (factor for inversion stimulation)...
  45. ncbi Antagonistic involvement of FIS and H-NS proteins in the transcriptional control of hns expression
    M Falconi
    Department of Biology, University of Camerino, Italy
    Mol Microbiol 19:965-75. 1996
    Gel shift and DNase I footprinting experiments showed that Escherichia coli FIS (factor for inversion stimulation) protein binds to at least seven sites in the promoter region of hns...
  46. pmc Contributions of UP elements and the transcription factor FIS to expression from the seven rrn P1 promoters in Escherichia coli
    C A Hirvonen
    Department of Bacteriology, University of Wisconsin Madison, Madison, Wisconsin 53706, USA
    J Bacteriol 183:6305-14. 2001
    ..Escherichia coli results from a cis-acting DNA sequence, the UP element, and a trans-acting transcription factor, FIS. In this study, we examine the effects of FIS and the UP element at the other six rrn P1 promoters...
  47. pmc Molecular flip-flops formed by overlapping Fis sites
    Paul N Hengen
    Intramural Research Support Program, SAIC, NCI Frederick, Frederick, MD 21702 1201, USA
    Nucleic Acids Res 31:6663-73. 2003
    The DNA-binding protein Fis frequently uses pairs of sites 7 or 11 base pairs (bp) apart. Two overlapping Fis sites separated by 11 bp are found in the Escherichia coli origin of chromosomal replication...
  48. pmc Control of rRNA synthesis in Escherichia coli: a systems biology approach
    Patrick P Dennis
    Division of Molecular and Cellular Biosciences, National Science Foundation, 4201 Wilson Blvd, Arlington VA 22230, USA
    Microbiol Mol Biol Rev 68:639-68. 2004
    ..per minute per promoter in bacterial strains either deficient or proficient in the synthesis of the factor Fis and/or the effector ppGpp...
  49. pmc Correlation between binding rate constants and individual information of E. coli Fis binding sites
    Ryan K Shultzaberger
    National Cancer Institute at Frederick, Center for Cancer Research Nanobiology Program, SAIC Frederick, NCI Frederick Bldg 469, Rm 237 Frederick, MD 21782, USA
    Nucleic Acids Res 35:5275-83. 2007
    ..we used surface plasmon resonance and electromobility shift assays to determine the kinetics for binding of the Fis protein to a range of naturally occurring binding sites...
  50. doi Competition between NarL-dependent activation and Fis-dependent repression controls expression from the Escherichia coli yeaR and ogt promoters
    Derrick J P Squire
    University of Birmingham, Edgbaston, UK
    Biochem J 420:249-57. 2009
    ..5. NarL-dependent activation at both the yeaR and ogt promoters is decreased in rich medium and this depends on Fis, a nucleoid-associated protein...
  51. pmc Changes in Escherichia coli rRNA promoter activity correlate with changes in initiating nucleoside triphosphate and guanosine 5' diphosphate 3'-diphosphate concentrations after induction of feedback control of ribosome synthesis
    David A Schneider
    Department of Bacteriology, University of Wisconsin, Madison, Wisconsin 53706, USA
    J Bacteriol 185:6185-91. 2003
    ..In contrast, we observed no change in the level of DNA supercoiling under the feedback conditions examined...
  52. pmc Fis plays a role in Tn5 and IS50 transposition
    M D Weinreich
    Department of Biochemistry, University of Wisconsin Madison 53706
    J Bacteriol 174:4530-7. 1992
    The Fis (factor for inversion stimulation) protein of Escherichia coli was found to influence the frequency of transposon Tn5 and insertion sequence IS50 transposition...
  53. ncbi Potential binding sites of the trans-activator FIS are present upstream of all rRNA operons and of many but not all tRNA operons
    H Verbeek
    Department of Biochemistry, Leiden University, Gorleaus Laboratories, The Netherlands
    Biochim Biophys Acta 1050:302-6. 1990
    b>FIS, the Escherichia coli protein that stimulates the inversion of various DNA segments by binding to a recombinational enhancer, trans-activates a number of stable RNA operons and binds to the upstream activator sequence (UAS) of these ..
  54. pmc Identification of genes negatively regulated by Fis: Fis and RpoS comodulate growth-phase-dependent gene expression in Escherichia coli
    J Xu
    Department of Biological Chemistry, UCLA School of Medicine 90024
    J Bacteriol 177:938-47. 1995
    b>Fis is a nucleoid-associated protein in Escherichia coli that has been shown to regulate recombination, replication, and transcription reactions...
  55. pmc Escherichia coli Fis and DnaA proteins bind specifically to the nrd promoter region and affect expression of an nrd-lac fusion
    L B Augustin
    Department of Biochemistry, University of Minnesota, St Paul 55108
    J Bacteriol 176:378-87. 1994
    ..coli factor for inversion stimulation (Fis)...
  56. ncbi Expression of the Fis protein is sustained in late-exponential- and stationary-phase cultures of Salmonella enterica serovar Typhimurium grown in the absence of aeration
    Tadhg O Cróinín
    Department of Microbiology, Moyne Institute of Preventive Medicine, Trinity College Dublin, Ireland
    Mol Microbiol 66:237-51. 2007
    The classic expression pattern of the Fis global regulatory protein during batch culture consists of a high peak in the early logarithmic phase of growth, followed by a sharp decrease through mid-exponential growth phase until Fis is ..
  57. doi Carbon monoxide-releasing antibacterial molecules target respiration and global transcriptional regulators
    Kelly S Davidge
    Department of Molecular Biology and Biotechnology, The University of Sheffield, Firth Ct, Western Bank, Sheffield S10 2TN, United Kingdom
    J Biol Chem 284:4516-24. 2009
    ..Probabilistic modeling of transcriptomic data identified the global transcription regulators ArcA, CRP, Fis, FNR, Fur, BaeR, CpxR, and IHF as targets and potential CO sensors...
  58. doi Transcription of the plasmid-encoded toxin gene from enteroaggregative Escherichia coli is regulated by a novel co-activation mechanism involving CRP and Fis
    Amanda E Rossiter
    School of Immunity and Infection School of Biosciences, University of Birmingham, Birmingham B15 2TT, UK
    Mol Microbiol 81:179-91. 2011
    ..Following a global transposon mutagenesis screen of EAEC 042, the transcription factors, CRP and Fis, were identified as essential for transcription of the pet gene...
  59. pmc Both fis-dependent and factor-independent upstream activation of the rrnB P1 promoter are face of the helix dependent
    J T Newlands
    Department of Bacteriology, University of Wisconsin, Madison 53706
    Nucleic Acids Res 20:719-26. 1992
    ..Activation by the UAR involves two components: (1) a trans-acting protein, Fis, which binds to three sites in the UAR between -60 and -150, and (2) the UAR sequences themselves which affect RNA ..
  60. pmc Membrane topology analysis of Escherichia coli mannitol permease by using a nested-deletion method to create mtlA-phoA fusions
    J E Sugiyama
    Department of Biology, Boston University, MA 02215
    Proc Natl Acad Sci U S A 88:9603-7. 1991
    ....
  61. pmc Fis activates the RpoS-dependent stationary-phase expression of proP in Escherichia coli
    J Xu
    Department of Biological Chemistry, UCLA School of Medicine 90095, USA
    J Bacteriol 177:5222-31. 1995
    b>Fis is a general nucleoid-associated protein in Escherichia coli whose expression is highly regulated with respect to growth conditions...
  62. ncbi Effects of Fis on ribosome synthesis and activity and on rRNA promoter activities in Escherichia coli
    X Zhang
    Molecular Program, University of Texas at Dallas, Richardson 75083 0688, USA
    J Mol Biol 259:27-40. 1996
    The DNA-bending protein Fis of Escherichia coli is required for efficient initiation of chromosome replication and for activation of the P1 promoters of stable RNA (rRNA and tRNA) genes...
  63. pmc Location, degree, and direction of DNA bending associated with the Hin recombinational enhancer sequence and Fis-enhancer complex
    D Perkins-Balding
    Department of Microbiology and Immunology, Emory University School of Medicine, Atlanta, Georgia 30322, USA
    J Bacteriol 179:4747-53. 1997
    The Fis protein of Escherichia coli and Salmonella typhimurium stimulates several site-specific DNA recombination reactions, as well as transcription of a number of genes...
  64. ncbi Induction and repair of cyclobutane pyrimidine dimers in the Escherichia coli tRNA gene tyrT: Fis protein affects dimer induction in the control region and suppresses preferential repair in the coding region of the transcribed strand, except in a short re
    S Li
    School of Biological Sciences, University of Wales Swansea, UK
    J Mol Biol 271:31-46. 1997
    ..In wild-type (WT) log or stationary phase different patterns of induction occurred in the three Fis binding sites and the core promoter -35 sequence of the control region: this was absent in fis- cells...
  65. pmc The transactivation region of the fis protein that controls site-specific DNA inversion contains extended mobile beta-hairpin arms
    M K Safo
    Institute of Molecular Biology, Academia Sinica, Taipei, Taiwan 11529, Republic of China
    EMBO J 16:6860-73. 1997
    The Fis protein regulates site-specific DNA inversion catalyzed by a family of DNA invertases when bound to a cis-acting recombinational enhancer...
  66. ncbi Structural analysis of the transcriptional activation region on Fis: crystal structures of six Fis mutants with different activation properties
    Y S Cheng
    Graduate Institute of Life Science, National Defense Medical Center, Taipei, Taiwan, Republic of China
    J Mol Biol 302:1139-51. 2000
    The Fis protein regulates gene expression in Escherichia coli by activating or repressing transcription of a variety of genes...
  67. pmc Positive growth rate-dependent regulation of the pdxA, ksgA, and pdxB genes of Escherichia coli K-12
    Andrew J Pease
    Department of Microbiology and Molecular Genetics, University of Texas Houston Medical School, Houston, TX 77030 1501, USA
    J Bacteriol 184:1359-69. 2002
    ..On the other hand, growth rate regulation of the amount of the pdxA-ksgA cotranscript was abolished by a fis mutation, suggesting a role for the Fis protein...
  68. pmc Transcriptional regulation of fis operon involves a module of multiple coupled promoters
    William Nasser
    Max Planck Institute for Terrestrial Microbiology, Karl von Frisch Strasse, D 35043 Marburg, Germany
    EMBO J 21:715-24. 2002
    The transcription of the Escherichia coli fis gene is strongly activated during the outgrowth of cells from stationary phase. The high activity of the promoter of the fis operon requires the transcription factor IHF...
  69. pmc The Escherichia coli Fis protein stimulates bacteriophage lambda integrative recombination in vitro
    Dominic Esposito
    Invitrogen Corporation, Frederick, Maryland 21704, USA
    J Bacteriol 185:3076-80. 2003
    The Escherichia coli nucleoid-associated protein Fis was previously shown to be involved in bacteriophage lambda site-specific recombination in vivo, enhancing the levels of both integrative recombination and excisive recombination...
  70. ncbi A unifying model for the role of polyamines in bacterial cell growth, the polyamine modulon
    Madoka Yoshida
    Graduate School of Pharmaceutical Sciences, Chiba University, 1 8 1 Inohana, Chuo Ku, Chiba 260 8675, Japan
    J Biol Chem 279:46008-13. 2004
    ..In this study we found that the synthesis of FecI and Fis was also stimulated by polyamines at the level of translation...
  71. ncbi Illegitimate recombination mediated by double-strand break and end-joining in Escherichia coli
    Hideo Ikeda
    Institute of Molecular Genetics, Medinet Inc, Tamagawadai 2 2 8, Setagaya Ku, Tokyo 158 0096, Japan
    Adv Biophys 38:3-20. 2004
    ..RecQ plays a role in the suppression of RecEmediated illegitimate recombination, with the participation of DnaB, Fis, Exol, and H-NS...
  72. pmc Long-term experimental evolution in Escherichia coli. XII. DNA topology as a key target of selection
    Estelle Crozat
    Laboratoire Adaptation et Pathogenie des Microorganismes, Universite Joseph Fourier, Institut Jean Roget, CNRS UMR 5163, F 38041 Grenoble, France
    Genetics 169:523-32. 2005
    ..Two mutations in the topA and fis genes that control supercoiling were discovered in a population that served as the focus for further investigation...
  73. ncbi Illegitimate recombination mediated by double-strand break and end-joining in Escherichia coli
    Hideo Ikeda
    Institute of Molecular Genetics, Medinet Inc, Tamagawadai 2 2 8, Setagaya Ku, Tokyo 158 0096, Japan
    Adv Biophys 38:3-20. 2004
    ..RecQ plays a role in the suppression of RecE-mediated illegitimate recombination, with the participation of DnaB, Fis, ExoI, and H-NS...
  74. ncbi Control of ribosome synthesis during the cell division cycles of E. coli and Synechococcus
    Yukio Asato
    University of Massachusetts Dartmouth, N Dartmouth, Massachusetts 02747, USA
    Curr Issues Mol Biol 7:109-17. 2005
    ..UP) element, molecular effectors, ppGpp and iNTP (initiating nucleoside triphosphate), and trans-acting proteins, Fis and H-NS, play important roles in the control of rRNA synthesis in response to changing nutritional environments...
  75. ncbi Escherichia coli ribosomal RNA transcription: regulatory roles for ppGpp, NTPs, architectural proteins and a polymerase-binding protein
    Jay D Gralla
    Department of Chemistry and Biochemistry, Molecular Biology Institute, University of California, Los Angeles, CA 90095 1569, USA
    Mol Microbiol 55:973-7. 2005
    ..This review discusses the current state of affairs, including recent information about the involvement of two nucleotide regulators, two architectural protein regulators, one new co-regulator and stalled ribosomes...
  76. ncbi Differential regulation of Escherichia coli topoisomerase I by Fis
    Dalit Weinstein-Fischer
    Department of Molecular Genetics and Biotechnology, The Hebrew University Hadassah Medical School, Jerusalem, Israel
    Mol Microbiol 63:1131-44. 2007
    ..P1 promoter of topA encoding topoisomerase I of Escherichia coli is activated in response to oxidative stress, in a Fis-dependent manner. Here we show that Fis regulation of topA varies with the intracellular concentrations of Fis...
  77. ncbi Inhibition of IS2 transposition by factor for inversion stimulation
    Guang Sheng Lei
    Graduate Institute of Microbiology and Immunology, National Yang Ming University, Taipei, Taiwan, Republic of China
    FEMS Microbiol Lett 275:98-105. 2007
    The effect of factor for inversion stimulation (Fis) protein on IS2 transposition was investigated. A full-length IS2 was found to transpose at a frequency 64 times lower in a normal Escherichia coli than in a fis- mutant...
  78. pmc Role of global regulators and nucleotide metabolism in antibiotic tolerance in Escherichia coli
    Sonja Hansen
    Antimicrobial Discovery Center and Department of Biology, Northeastern University, Boston, MA 02115, USA
    Antimicrob Agents Chemother 52:2718-26. 2008
    ..were dnaJ and dnaK (chaperones), apaH (diadenosine tetraphosphatase), surA (peptidyl-prolyl cis-trans isomerase), fis and hns (global regulators), hnr (response regulator of RpoS), dksA (transcriptional regulator of rRNA ..
  79. pmc Hypernegative supercoiling inhibits growth by causing RNA degradation
    Imad Baaklini
    Departement de Microbiologie et Immunologie, Universite de Montreal, 2900 Edouard Montpetit, Room R 612, Montreal, Quebec H3C 3J7, Canada
    J Bacteriol 190:7346-56. 2008
    ..ribosomes in extracts showed normal activity when obtained during growth arrest, mRNA on ribosomes was reduced for fis and shorter for crp, polysomes were much less abundant relative to monosomes, and protein synthesis rate dropped, ..
  80. pmc Synergistic role of curli and cellulose in cell adherence and biofilm formation of attaching and effacing Escherichia coli and identification of Fis as a negative regulator of curli
    Zeus Saldaña
    Department of Immunobiology, University of Arizona, 1501 N Tucson, AZ 85724, USA
    Environ Microbiol 11:992-1006. 2009
    ..Analysis of the influence of virulence and global regulators in the production of curli in EPEC identified Fis (factor for inversion stimulation) as a, heretofore unrecognized, negative transcriptional regulator of csgA ..
  81. doi Nucleoid-associated proteins and bacterial physiology
    Charles J Dorman
    Department of Microbiology, School of Genetics and Microbiology, Trinity College, Dublin 2, Ireland
    Adv Appl Microbiol 67:47-64. 2009
    ..The nucleoid-associated proteins, especially Factor for Inversion Stimulation (Fis), IHF, H-NS, HU, and Lrp, represent a prominent group of global regulators and studies of these proteins and their ..
  82. pmc Role of decreased levels of Fis histone-like protein in Crohn's disease-associated adherent invasive Escherichia coli LF82 bacteria interacting with intestinal epithelial cells
    Sylvie Miquel
    Clermont Universite, Universite d Auvergne, Clermont Ferrand, France
    J Bacteriol 192:1832-43. 2010
    ..in AIEC reference strain LF82 during infection of intestinal epithelial cells, Intestine-407, and observed that the fis mRNA level was decreased...
  83. ncbi Crystal structure of the factor for inversion stimulation FIS at 2.0 A resolution
    D Kostrewa
    Institut fur Kristallographie, Freie Universitat Berlin, Germany
    J Mol Biol 226:209-26. 1992
    The factor for inversion stimulation (FIS) binds as a homodimeric molecule to a loose 15 nucleotide consensus sequence in DNA...
  84. pmc The role of FIS in trans activation of stable RNA operons of E. coli
    L Nilsson
    Department of Biochemistry, Leiden University, Gorlaeus Laboratories, The Netherlands
    EMBO J 9:727-34. 1990
    ..We demonstrate that the E.coli protein FIS (Factor for Inversion Stimulation) also binds to the UAS of the thrU(tufB) operon forming three protein-DNA ..
  85. ncbi Three-dimensional structure of the E. coli DNA-binding protein FIS
    D Kostrewa
    Institut fur Kristallographie, Freie Universitat Berlin, Germany
    Nature 349:178-80. 1991
    The factor for inversion stimulation, FIS, is involved in several cellular processes, including site-specific recombination and transcriptional activation...
  86. pmc Isolation of the gene encoding the Hin recombinational enhancer binding protein
    R C Johnson
    Department of Biological Chemistry, School of Medicine, University of California, Los Angeles 90024
    Proc Natl Acad Sci U S A 85:3484-8. 1988
    ..presence of a recombinational enhancer sequence located in cis relative to the recombination sites and a protein, Fis, which binds to the enhancer. We have cloned and determined the primary sequence of the gene encoding Fis...
  87. pmc Fis, an accessorial factor for transcriptional activation of the mar (multiple antibiotic resistance) promoter of Escherichia coli in the presence of the activator MarA, SoxS, or Rob
    R G Martin
    Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, Maryland 20892 0560, USA
    J Bacteriol 179:7410-9. 1997
    ..The accessory region is shown here to be a binding site for Fis centered at -81 and to bind Fis, a small DNA-binding and -bending protein, with a Kd of approximately 5 nM...
  88. ncbi Influence of FIS on the transcription from closely spaced and non-overlapping divergent promoters for an aminoacyl-tRNA synthetase gene (gltX) and a tRNA operon (valU) in Escherichia coli
    N Champagne
    Departement de Biochimie, Faculte des sciences et de genie, Universite Laval, Quebec, Canada
    Mol Microbiol 27:1141-56. 1998
    ..report that their promoter region (250 bp) contains three binding sites for the factor for inversion stimulation (FIS), centred at positions -71, -91 and -112 from the valU transcription initiation site, and that the destruction of ..
  89. ncbi Fis stabilizes the interaction between RNA polymerase and the ribosomal promoter rrnB P1, leading to transcriptional activation
    Huijun Zhi
    Laboratory of Molecular Biology, National Cancer Institute NIH, 9000 Rockville Pike, Bethesda, MD 20892, USA
    J Biol Chem 278:47340-9. 2003
    It has been shown that Fis activates transcription of the ribosomal promoter rrnB P1; however, the mechanism by which Fis activates rrnB P1 transcription is not fully understood...
  90. ncbi Phosphorylation-independent dimer-dimer interactions by the enhancer-binding activator NtrC of Escherichia coli: a third function for the C-terminal domain
    Xiaofeng F Yang
    Molecular and Cell Biology Department, University of Texas at Dallas, Richardson, TX 75083 0688, USA
    J Biol Chem 279:36708-14. 2004
    ..We discuss the differences and similarities between the NtrC C-terminal domain and the homologous Fis, which is also capable of intermolecular interactions.
  91. pmc A dedicated translation factor controls the synthesis of the global regulator Fis
    Roisin M Owens
    School of Biological Sciences, University of Southampton, Southampton, UK
    EMBO J 23:3375-85. 2004
    ..functions as a translation factor that is required specifically for the expression of the transcriptional modulator Fis. BipA binds to ribosomes at a site that coincides with that of elongation factor G and has a GTPase activity that ..
  92. ncbi Polyamine Modulon in Escherichia coli: genes involved in the stimulation of cell growth by polyamines
    Kazuei Igarashi
    Graduate School of Pharmaceutical Sciences, Chiba University, 1 8 1 Inohana, Chiba 260 8675
    J Biochem 139:11-6. 2006
    ..operon (FecI), and transcription factor of growth-related genes including rRNA and some kinds of tRNA synthesis (Fis) was enhanced by polyamines at the level of translation...
  93. ncbi The architectural role of nucleoid-associated proteins in the organization of bacterial chromatin: a molecular perspective
    Martijn S Luijsterburg
    Department of Physics and Astronomy, Faculty of Sciences, Vrije Universiteit, Amsterdam, De Boelelaan 1081, 1081 HV Amsterdam, The Netherlands
    J Struct Biol 156:262-72. 2006
    ..In this review, we provide an overview of the major nucleoid-associated proteins from a structural perspective and we discuss their possible roles in dynamically shaping the bacterial nucleoid...
  94. doi Studies on the expression of 6S RNA from E. coli: involvement of regulators important for stress and growth adaptation
    Thomas Neusser
    Institut für Physikalische Biologie, Heinrich Heine Universitat Dusseldorf, Universitatsstr 1, D 40225 Dusseldorf, Germany
    Biol Chem 389:285-97. 2008
    ..To this aim, we have analyzed the effects of the typical bacterial growth phase and stress regulators FIS, H-NS, LRP and StpA on 6S RNA expression...
  95. doi Fine-tuned growth phase control of dps, encoding a DNA protection protein, by FIS and H-NS
    Karin Schnetz
    Institute for Genetics, University of Cologne, Cologne, Germany
    Mol Microbiol 68:1345-7. 2008
    ..In this issue of Molecular Microbiology, Grainger and co-workers demonstrate that the nucleoid-associated proteins, FIS and H-NS, control sigma factor selection at the dps promoter by a novel mechanism...
  96. ncbi Regulation of transcription at the ndh promoter of Escherichia coli by FNR and novel factors
    J Green
    Department of Molecular Biology and Biotechnology, University of Sheffield, UK
    Mol Microbiol 12:433-44. 1994
    ..These putative amino acid-responsive activators may thus offset FNR-mediated repression and maintain a low level of anaerobic ndh expression for regulating the NAD+/NADH ratio during growth in rich media...
  97. pmc DNA binding and bending are necessary but not sufficient for Fis-dependent activation of rrnB P1
    K K Gosink
    Department of Bacteriology, University of Wisconsin, Madison 53706
    J Bacteriol 175:1580-9. 1993
    The Escherichia coli Fis protein binds to three sites in the upstream activation region of the rrnB P1 promoter and enhances transcription 5- to 10-fold in vivo...
  98. pmc A positive control mutant of the transcription activator protein FIS
    K K Gosink
    Department of Bacteriology, University of Wisconsin, Madison 53706, USA
    J Bacteriol 178:5182-7. 1996
    The FIS protein is a transcription activator of rRNA and other genes in Escherichia coli...
  99. pmc FIS activates sequential steps during transcription initiation at a stable RNA promoter
    G Muskhelishvili
    Institut fur Genetik und Mikrobiologie, LMU, Munchen, Germany
    EMBO J 16:3655-65. 1997
    b>FIS (factor for inversion stimulation) is a small dimeric DNA-bending protein which both stimulates DNA inversion and activates transcription at stable RNA promoters in Escherichia coli...
  100. ncbi The expression of the Escherichia coli fis gene is strongly dependent on the superhelical density of DNA
    R Schneider
    Institut fur Genetik und Mikrobiologie, LMU Munchen, Maria Ward Str 1a, 80638 Munchen, Germany
    Mol Microbiol 38:167-75. 2000
    The Escherichia coli DNA architectural protein FIS is a pleiotropic regulator, which couples the cellular physiology with transitions in the superhelical density of bacterial DNA...
  101. pmc Regulation of rRNA transcription correlates with nucleoside triphosphate sensing
    M M Barker
    Department of Bacteriology, University of Wisconsin, Madison, Wisconsin 53706, USA
    J Bacteriol 183:6315-23. 2001
    ..observed a correlation between NTP sensing in vitro and the response of the promoters in vivo to deletion of the fis gene (an example of homeostatic control), although this relationship was not as tight as for growth rate-dependent ..