ffh

Summary

Gene Symbol: ffh
Description: Signal Recognition Particle (SRP) component with 4.5S RNA (ffs)
Alias: ECK2607, JW5414
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. ncbi Protein traffic in bacteria: multiple routes from the ribosome to and across the membrane
    M Muller
    Institute of Biochemistry and Molecular Biology, University of Freiburg, Germany
    Prog Nucleic Acid Res Mol Biol 66:107-57. 2001
  2. pmc The targeting pathway of Escherichia coli presecretory and integral membrane proteins is specified by the hydrophobicity of the targeting signal
    H C Lee
    Genetics and Biochemistry Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Building 10, Room 9D 20, Bethesda, MD 20892 1810, USA
    Proc Natl Acad Sci U S A 98:3471-6. 2001
  3. ncbi Targeting and insertion of heterologous membrane proteins in E. coli
    Amanda Raine
    Department of Pharmaceutical Biosciences, Uppsala University, BMC, Box 591, 75124, Uppsala, Sweden
    Biochimie 85:659-68. 2003
  4. pmc Physiological basis for conservation of the signal recognition particle targeting pathway in Escherichia coli
    H D Bernstein
    Genetics and Biochemistry Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892 1810, USA
    J Bacteriol 183:2187-97. 2001
  5. pmc Co-translational protein targeting catalyzed by the Escherichia coli signal recognition particle and its receptor
    T Powers
    Department of Biochemistry and Biophysics, University of California, School of Medicine, San Francisco 94143, USA
    EMBO J 16:4880-6. 1997
  6. pmc The signal recognition particle receptor of Escherichia coli (FtsY) has a nucleotide exchange factor built into the GTPase domain
    C Moser
    Structural Biology Programme, European Molecular Biology Laboratory, Meyerhofstrasse 1, D 69012 Heidelberg, Germany
    Proc Natl Acad Sci U S A 94:11339-44. 1997
  7. ncbi Interaction of guanine nucleotides with the signal recognition particle from Escherichia coli
    J R Jagath
    Institute of Molecular Biology, University of Witten Herdecke, Germany
    Biochemistry 37:15408-13. 1998
  8. pmc In vitro studies with purified components reveal signal recognition particle (SRP) and SecA/SecB as constituents of two independent protein-targeting pathways of Escherichia coli
    H G Koch
    Institut fur Biochemie und Molekularbiologie, Universitat Freiburg, D 79104 Freiburg, Germany
    Mol Biol Cell 10:2163-73. 1999
  9. ncbi Substrate twinning activates the signal recognition particle and its receptor
    Pascal F Egea
    Department of Biochemistry and Biophysics, University of California at San Francisco, California 94143 2240, USA
    Nature 427:215-21. 2004
  10. ncbi Crystal structure of the ribonucleoprotein core of the signal recognition particle
    R T Batey
    Department of Molecular Biophysics and Biochemistry, Howard Hughes Medical Institute, Yale University, New Haven, CT 06511, USA
    Science 287:1232-9. 2000

Scientific Experts

  • Joen Luirink
  • T Powers
  • Taeho Ahn
  • Q A Valent
  • H D Bernstein
  • G Lentzen
  • Shu ou Shan
  • Pamela J Focia
  • Wolfgang Wintermeyer
  • Marina V Rodnina
  • Iwona Buskiewicz
  • Niels Bradshaw
  • Shan Qing Gu
  • Peter Walter
  • Sandra Angelini
  • Edith N G Houben
  • Ronald S Ullers
  • Gottfried Eisner
  • Bauke Oudega
  • Nenad Ban
  • Christiane Schaffitzel
  • J W de Gier
  • Johannes Jöckel
  • M Muller
  • Hans Georg Koch
  • Matthias Muller
  • Corinne M ten Hagen-Jongman
  • Frank Peske
  • Pascal F Egea
  • Sandro F Ataide
  • P Peluso
  • Ido Yosef
  • Amanda Raine
  • Jianying Luo
  • J R Jagath
  • Xin Zhang
  • Eugenia M Clerico
  • Stefan Kol
  • Thomas Bornemann
  • Jennifer A Doudna
  • Edwin van Bloois
  • Ebbe Sloth Andersen
  • K Beck
  • Nobuyuki Shimohata
  • Yutetsu Kuruma
  • Nellie Harms
  • H G Koch
  • Konstanze Beck
  • Liang Yi
  • Benjamin Ezraty
  • Robert M Stroud
  • Madi Bidya Sagar
  • Hans Joachim Wieden
  • Feixia Chu
  • Christina Wilson Bowers
  • Joseph Brunner
  • Janine H Peterson
  • Robert Sijbrandi
  • Clark F Schierle
  • P Walter
  • Mans Ehrenberg
  • Ross E Dalbey
  • N D Ulbrandt
  • Jon Beckwith
  • Olga N Avdeeva
  • Carleen A Brunelli
  • Jutta Rinke-Appel
  • Hongping Tian
  • Sei Kyoung Park
  • Richard Brimacombe
  • J F Swain
  • H C Lee
  • J Kim
  • Y Lu
  • C E Caldon
  • Kuang Shen
  • S Nock
  • D Herschlag
  • Nikolaus Schmitz
  • Ailong Ke
  • Julia Adler
  • A A Herskovits
  • Eitan Bibi
  • H Tian
  • R T Batey
  • Elena S Bochkareva
  • Julie Choulet
  • Aneta Szymanska
  • Nico Nouwen
  • J Macfarlane

Detail Information

Publications91

  1. ncbi Protein traffic in bacteria: multiple routes from the ribosome to and across the membrane
    M Muller
    Institute of Biochemistry and Molecular Biology, University of Freiburg, Germany
    Prog Nucleic Acid Res Mol Biol 66:107-57. 2001
    ..between their noncleaved signal anchor sequences and the bacterial signal recognition particle (SRP) consisting of Ffh and 4.5S RNA...
  2. pmc The targeting pathway of Escherichia coli presecretory and integral membrane proteins is specified by the hydrophobicity of the targeting signal
    H C Lee
    Genetics and Biochemistry Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Building 10, Room 9D 20, Bethesda, MD 20892 1810, USA
    Proc Natl Acad Sci U S A 98:3471-6. 2001
    ..These results imply that different classes of E. coli proteins are targeted by distinct pathways because bacterial SRP binds to a more restricted range of targeting signals than its eukaryotic counterpart...
  3. ncbi Targeting and insertion of heterologous membrane proteins in E. coli
    Amanda Raine
    Department of Pharmaceutical Biosciences, Uppsala University, BMC, Box 591, 75124, Uppsala, Sweden
    Biochimie 85:659-68. 2003
    ..coli membrane proteins. No significant pausing of protein elongation was observed in the presence of E. coli SRP, in line with the suggestion that translational arrest requires an Alu domain, which is absent in SRP from E. coli...
  4. pmc Physiological basis for conservation of the signal recognition particle targeting pathway in Escherichia coli
    H D Bernstein
    Genetics and Biochemistry Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892 1810, USA
    J Bacteriol 183:2187-97. 2001
    ..coli and possibly conserved throughout prokaryotic evolution as well partly because efficient IMP targeting prevents a toxic accumulation of aggregated proteins in the cytoplasm...
  5. pmc Co-translational protein targeting catalyzed by the Escherichia coli signal recognition particle and its receptor
    T Powers
    Department of Biochemistry and Biophysics, University of California, School of Medicine, San Francisco 94143, USA
    EMBO J 16:4880-6. 1997
    The Ffh-4.5S ribonucleoprotein particle (RNP) and FtsY from Escherichia coli are homologous to essential components of the mammalian signal recognition particle (SRP) and SRP receptor, respectively. The ability of these E...
  6. pmc The signal recognition particle receptor of Escherichia coli (FtsY) has a nucleotide exchange factor built into the GTPase domain
    C Moser
    Structural Biology Programme, European Molecular Biology Laboratory, Meyerhofstrasse 1, D 69012 Heidelberg, Germany
    Proc Natl Acad Sci U S A 94:11339-44. 1997
    ..In E. coli SRP consists of only one polypeptide (Ffh), and a 4.5S RNA...
  7. ncbi Interaction of guanine nucleotides with the signal recognition particle from Escherichia coli
    J R Jagath
    Institute of Molecular Biology, University of Witten Herdecke, Germany
    Biochemistry 37:15408-13. 1998
    ..In Escherichia coli, the particle consists of a 114 nt RNA, a 4.5S RNA, and a 48 kDa GTP-binding protein, Ffh. GDP-GTP exchange on, and GTP hydrolysis by, Ffh are thought to regulate SRP function in membrane targeting of ..
  8. pmc In vitro studies with purified components reveal signal recognition particle (SRP) and SecA/SecB as constituents of two independent protein-targeting pathways of Escherichia coli
    H G Koch
    Institut fur Biochemie und Molekularbiologie, Universitat Freiburg, D 79104 Freiburg, Germany
    Mol Biol Cell 10:2163-73. 1999
    ..was simultaneously rendered deficient in SecA/SecB and the signal recognition particle (SRP) components, Ffh (P48), 4. 5S RNA, and FtsY. The integration of two membrane proteins into inside-out plasma membrane vesicles of E...
  9. ncbi Substrate twinning activates the signal recognition particle and its receptor
    Pascal F Egea
    Department of Biochemistry and Biophysics, University of California at San Francisco, California 94143 2240, USA
    Nature 427:215-21. 2004
    ..This unique circle of twinned interactions is severed twice on hydrolysis, leading to complex dissociation after cargo delivery...
  10. ncbi Crystal structure of the ribonucleoprotein core of the signal recognition particle
    R T Batey
    Department of Molecular Biophysics and Biochemistry, Howard Hughes Medical Institute, Yale University, New Haven, CT 06511, USA
    Science 287:1232-9. 2000
    ..The structure explains why the conserved residues in the protein and RNA are required for SRP assembly and defines a signal sequence recognition surface composed of both protein and RNA...
  11. pmc A mutant hunt for defects in membrane protein assembly yields mutations affecting the bacterial signal recognition particle and Sec machinery
    H Tian
    Department of Microbiology and Molecular Genetics, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 97:4730-5. 2000
    ..Although both the ffs and the secA locus mutations interfere with membrane protein assembly, only the latter also reduces export of a protein containing a cleavable signal sequence...
  12. ncbi Role of 4.5S RNA in assembly of the bacterial signal recognition particle with its receptor
    P Peluso
    Howard Hughes Medical Institute and Department of Biochemistry and Biophysics, University of California, San Francisco, CA 94143, USA
    Science 288:1640-3. 2000
    ..In Escherichia coli, this reaction is mediated by the Ffh/4.5S RNA ribonucleoprotein complex (Ffh/4.5S RNP; the SRP) and the FtsY protein (the SRP receptor)...
  13. ncbi New prospects in studying the bacterial signal recognition particle pathway
    A A Herskovits
    Department of Biological Chemistry, Weizmann Institute of Science, Rehovot 76100, Israel
    Mol Microbiol 38:927-39. 2000
    ..The bacterial SRP system consists of at least two proteins and an RNA molecule (termed Ffh, FtsY and 4.5S RNA, respectively, in Escherichia coli)...
  14. pmc Differential use of the signal recognition particle translocase targeting pathway for inner membrane protein assembly in Escherichia coli
    J W de Gier
    Department of Biochemistry, Arrhenius Laboratory, Stockholm University, S 106 91 Stockholm University, Sweden
    Proc Natl Acad Sci U S A 95:14646-51. 1998
    ..in vitro and in Escherichia coli strains that are conditional for the expression of either the 54 homologue (Ffh) or 4.5S RNA, which are the two components of the E...
  15. ncbi Biogenesis of inner membrane proteins in Escherichia coli
    J W de Gier
    Department of Biochemistry, Stockholm University, 106 91 Stockholm, Sweden
    Mol Microbiol 40:314-22. 2001
    ..Here, we review and discuss recent advances in our understanding of the biogenesis of inner membrane proteins in E. coli...
  16. pmc The DsbA signal sequence directs efficient, cotranslational export of passenger proteins to the Escherichia coli periplasm via the signal recognition particle pathway
    Clark F Schierle
    Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Bacteriol 185:5706-13. 2003
    ..Finally, probably because of its rapid folding in the cytoplasm, thioredoxin provides, along with gene fusion approaches, a sensitive assay system for signal sequences that utilize the SRP pathway...
  17. ncbi Is Ffh required for export of secretory proteins?
    J Kim
    Department of Molecular and Cell Biology, University of Connecticut, Storrs, CT 06269 3044, USA
    FEBS Lett 505:245-8. 2001
    ..We show that depletion of SRP, but not trigger factor, affects all the mutants examined. These results suggest secretory proteins with a variety of signal sequences, as well as membrane proteins, require SRP for export...
  18. ncbi Role of SRP RNA in the GTPase cycles of Ffh and FtsY
    P Peluso
    Howard Hughes Medical Institute and Department of Biochemistry and Biophysics, University of California at San Francisco, 94143 0448, USA
    Biochemistry 40:15224-33. 2001
    The bacterial homologues of the signal recognition particle (SRP) and its receptor, the Ffh*4...
  19. pmc Genetic screen yields mutations in genes encoding all known components of the Escherichia coli signal recognition particle pathway
    Hongping Tian
    Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Bacteriol 184:111-8. 2002
    ..coli signal recognition particle pathway: ffh, ffs, and ftsY, which encode Ffh, 4.5S RNA, and FtsY, respectively...
  20. ncbi Basic amino acids in a distinct subset of signal peptides promote interaction with the signal recognition particle
    Janine H Peterson
    Genetics and Biochemistry Branch, NIDDK, National Institutes of Health, Bethesda, Maryland 20892 0538, USA
    J Biol Chem 278:46155-62. 2003
    ..Taken together, the data suggest that the formation of salt bridges between SRP RNA and basic amino acids facilitates the binding of a distinct subset of signal peptides whose hydrophobicity falls slightly below a threshold level...
  21. pmc Functional analysis of the signal recognition particle in Escherichia coli by characterization of a temperature-sensitive ffh mutant
    Sei Kyoung Park
    Department of Microbiology, 207 Science I Building, Iowa State University, Ames, IA 50011, USA
    J Bacteriol 184:2642-53. 2002
    The Ffh protein of Escherichia coli is a 48-kDa polypeptide that is homologous to the SRP54 subunit of the eukaryotic signal recognition particle (SRP)...
  22. pmc Interplay of signal recognition particle and trigger factor at L23 near the nascent chain exit site on the Escherichia coli ribosome
    Ronald S Ullers
    Department of Molecular Microbiology, Vrije Universiteit, 1081 HV Amsterdam, The Netherlands
    J Cell Biol 161:679-84. 2003
    ..The results suggest a role for L23 in the targeting of IMPs as an attachment site for TF and SRP that is close to the emerging nascent chain...
  23. pmc The signal recognition particle binds to protein L23 at the peptide exit of the Escherichia coli ribosome
    Shan Qing Gu
    Institute of Molecular Biology, University of Witten Herdecke, 58448 Witten, Germany
    RNA 9:566-73. 2003
    The signal recognition particle (SRP) from Escherichia coli, composed of Ffh protein and 4.5S RNA, mediates membrane targeting of translating ribosomes displaying a signal or signal-anchor sequence...
  24. pmc Concentrations of 4.5S RNA and Ffh protein in Escherichia coli: the stability of Ffh protein is dependent on the concentration of 4.5S RNA
    C G Jensen
    Department of Molecular Cell Biology, University of Copenhagen, Denmark
    J Bacteriol 176:7148-54. 1994
    We measured the concentrations of both 4.5S RNA and Ffh protein under a variety of growth conditions and found that there were 400 molecules of 4...
  25. ncbi Signal-sequence recognition by an Escherichia coli ribonucleoprotein complex
    J Luirink
    European Molecular Biology Laboratory, Heidelberg, Germany
    Nature 359:741-3. 1992
    ..5S RNA and P48 protein (Ffh) of Escherichia coli which form a ribonucleoprotein particle. Depletion of 4...
  26. pmc Membrane protein biogenesis in Ffh- or FtsY-depleted Escherichia coli
    Ido Yosef
    Department of Biological Chemistry, Weizmann Institute of Science, Rehovot, Israel
    PLoS ONE 5:e9130. 2010
    ..SRP) system is required for biogenesis of membrane proteins and contains two essential proteins: the SRP subunit Ffh and the SRP-receptor FtsY...
  27. ncbi Homology of 54K protein of signal-recognition particle, docking protein and two E. coli proteins with putative GTP-binding domains
    K Römisch
    European Molecular Biology Laboratory, Heidelberg, FRG
    Nature 340:478-82. 1989
    ..Significant sequence homology was found to part of DP alpha and two E. coli proteins of unknown function. The homologous region includes a putative GTP-binding domain...
  28. ncbi Model for signal sequence recognition from amino-acid sequence of 54K subunit of signal recognition particle
    H D Bernstein
    Department of Biochemistry and Biophysics, University of California Medical School, San Francisco 94143 0448
    Nature 340:482-6. 1989
    ..We propose that SRP54 and SR alpha use GTP in sequential steps of the targeting reaction and that essential features of such a pathway are conserved from bacteria to mammals...
  29. doi Signal sequence-independent membrane targeting of ribosomes containing short nascent peptides within the exit tunnel
    Thomas Bornemann
    Institute of Molecular Biology, University of Witten Herdecke, Stockumer Strasse 10, 55448 Witten, Germany
    Nat Struct Mol Biol 15:494-9. 2008
    ....
  30. ncbi The E. coli signal recognition particle is required for the insertion of a subset of inner membrane proteins
    N D Ulbrandt
    Genetics and Biochemistry Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
    Cell 88:187-96. 1997
    ..coli preproteins and some IMPs can utilize SRP-independent targeting pathways effectively, the structural features of a subset of IMPs have required the conservation of an SRP-based targeting machinery...
  31. ncbi Sequence-specific interactions of nascent Escherichia coli polypeptides with trigger factor and signal recognition particle
    Ronald S Ullers
    Department of Molecular Microbiology, Institute of Molecular Cell Biology, Vrije Universiteit, 1081 HV Amsterdam, The Netherlands
    J Biol Chem 281:13999-4005. 2006
    ..Importantly and in contrast to published data, the SRP appears perfectly capable of distinguishing SA sequences from signal sequences in secretory proteins at this early stage in biogenesis...
  32. ncbi Biogenesis of inner membrane proteins in Escherichia coli
    Joen Luirink
    Department of Microbiology, Institute of Molecular Cell Biology, Vrije Universiteit, 1081 HV Amsterdam, The Netherlands
    Annu Rev Microbiol 59:329-55. 2005
    ..This review deals mainly with recent structural and biochemical data from various experimental systems that offer new insight into the different stages of biogenesis of E. coli IMPs...
  33. ncbi Reciprocal stimulation of GTP hydrolysis by two directly interacting GTPases
    T Powers
    Department of Biochemistry and Biophysics, University of California Medical School, San Francisco 94143 0448, USA
    Science 269:1422-4. 1995
    The Escherichia coli guanosine triphosphate (GTP)-binding proteins Ffh and FtsY have been proposed to catalyze the cotranslational targeting of proteins to the bacterial plasma membrane...
  34. pmc FtsY, the bacterial signal-recognition particle receptor, interacts functionally and physically with the SecYEG translocon
    Sandra Angelini
    Institute for Biochemistry and Molecular Biology, University Freiburg, Hermann Herder Strasse 7, 79104 Freiburg, Germany
    EMBO Rep 6:476-81. 2005
    ..We now provide evidence for the direct interaction between FtsY and the SecY translocon. This interaction offers an explanation of how the bacterial SRP cycle is regulated in response to available translocation channels...
  35. ncbi Assembly of a cytoplasmic membrane protein in Escherichia coli is dependent on the signal recognition particle
    J W de Gier
    Department of Biochemistry, Arrhenius Laboratory, Stockholm University, Sweden
    FEBS Lett 399:307-9. 1996
    ..to the wild-type protein was studied in Escherichia coli strains that are conditional for the expression of either Ffh or 4.5S RNA, the two components of the E. coli SRP...
  36. pmc Mechanism of association and reciprocal activation of two GTPases
    Shu ou Shan
    Department of Biochemistry and Biophysics, University of California, San Francisco, USA
    PLoS Biol 2:e320. 2004
    ..During this process, two GTPases, one in SRP and one in the SRP receptor (named Ffh and FtsY in bacteria, respectively), form a complex in which both proteins reciprocally activate the GTPase ..
  37. ncbi The functional integration of a polytopic membrane protein of Escherichia coli is dependent on the bacterial signal-recognition particle
    J Macfarlane
    Institut für Physikalische Biochemie, Universitat Munchen, Germany
    Eur J Biochem 233:766-71. 1995
    ..5S RNA (Ffs) and 7SL RNA, and (b) the E. coli P48 (Ffh) and SRP 54-kDa subunit, more evidence has been obtained that E...
  38. pmc Early encounters of a nascent membrane protein: specificity and timing of contacts inside and outside the ribosome
    Edith N G Houben
    Department of Molecular Microbiology, Institute of Molecular Cell Biology, Vrije Universiteit, 1081 HV Amsterdam, Netherlands
    J Cell Biol 170:27-35. 2005
    ..Intraribosomal contacts of the nascent chain were not influenced by the presence of a functional TM in the ribosome...
  39. pmc Discrimination between SRP- and SecA/SecB-dependent substrates involves selective recognition of nascent chains by SRP and trigger factor
    K Beck
    Institut für Biochemie und Molekularbiologie und, Universitat Freiburg, Hermann Herder Strasse 7, D 79104 Freiburg, Germany
    EMBO J 19:134-43. 2000
    ....
  40. pmc Secretion of LamB-LacZ by the signal recognition particle pathway of Escherichia coli
    Christina Wilson Bowers
    Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544, USA
    J Bacteriol 185:5697-705. 2003
    ..Secretion of this hydrophobic fusion variant (H*LamB-LacZ) was reduced in the absence of fully functional Ffh and Ffs, and the translocator jamming caused by Hyb42-1 was prevented by efficient delivery of the fusion to the ..
  41. ncbi Nascent membrane and presecretory proteins synthesized in Escherichia coli associate with signal recognition particle and trigger factor
    Q A Valent
    Department of Microbiology, Institute of Molecular Biological Sciences, BioCentrum Amsterdam, The Netherlands
    Mol Microbiol 25:53-64. 1997
    ..coli ribosome...
  42. ncbi Domain interactions in E. coli SRP: stabilization of M domain by RNA is required for effective signal sequence modulation of NG domain
    N Zheng
    Molecular Biophysics Program, University of Texas Southwestern Medical Center at Dallas 75235, USA
    Mol Cell 1:79-87. 1997
    ..5S RNA. Signal peptide binding to isolated NG domain also causes a pronounced destabilization, implicating the NG domain in direct recognition of signal peptide...
  43. pmc Crosslinking of 4.5S RNA to the Escherichia coli ribosome in the presence or absence of the protein Ffh
    Jutta Rinke-Appel
    Max Planck Institut fur Molekulare Genetik, Berlin, Germany
    RNA 8:612-25. 2002
    ..The ability of this modified 4.5S RNA to form a complex with the protein Ffh was demonstrated by a gel shift assay. The modified 4...
  44. ncbi Construction of the 'minimal' SRP that interacts with the translating ribosome but not with specific membrane receptors in Escherichia coli
    Olga N Avdeeva
    Department of Chemistry, Moscow State University, 119899, Moscow, Russia
    FEBS Lett 514:70-3. 2002
    Escherichia coli signal recognition particle (SRP) consists of 4.5S RNA and Ffh protein. In contrast to eukaryotes, it remains unclear whether translation arrest takes place in prokaryotic cells...
  45. pmc Evidence for a novel GTPase priming step in the SRP protein targeting pathway
    Y Lu
    Genetics and Biochemistry Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Building 10, Room 9D 20, Bethesda, MD 20892 1810, USA
    EMBO J 20:6724-34. 2001
    ..of the SRP pathway GTPases, we mutated an invariant glycine in Escherichia coli SRP54 and SRalpha orthologs ('Ffh' and 'FtsY', respectively) that resides at the N-GTPase domain interface...
  46. pmc Signal sequences activate the catalytic switch of SRP RNA
    Niels Bradshaw
    Howard Hughes Medical Institute, 4000 Jones Bridge Road, Chevy Chase, MD 20815, USA
    Science 323:127-30. 2009
    ..Thus, SRP RNA is a molecular switch that renders the SRP-SR GTPase engine responsive to signal peptide recruitment, coupling GTP hydrolysis to productive protein targeting...
  47. pmc Trigger factor binds to ribosome-signal-recognition particle (SRP) complexes and is excluded by binding of the SRP receptor
    Iwona Buskiewicz
    Institute für Molekularbiologie und Physikalische Biochemie, Universitat Witten Herdecke, 58448 Witten, Germany
    Proc Natl Acad Sci U S A 101:7902-6. 2004
    ....
  48. pmc Multiple conformational switches in a GTPase complex control co-translational protein targeting
    Xin Zhang
    Division of Chemistry and Chemical Engineering, California Institute of Technology, 1200 East California Boulevard, Pasadena, CA 91125, USA
    Proc Natl Acad Sci U S A 106:1754-9. 2009
    ..Thus, the SRP and SR GTPases, without recruiting external regulatory factors, constitute a self-sufficient system that provides exquisite spatial and temporal control of a complex cellular process...
  49. pmc Exploring the interactions between signal sequences and E. coli SRP by two distinct and complementary crosslinking methods
    Eugenia M Clerico
    Department of Biochemistry and Molecular Biology, University of Massachusetts, Amherst MA 01003, U S A
    Biopolymers 92:201-11. 2009
    ..By PICUP, signal peptides crosslinked exclusively to the NG domain of the SRP protein Ffh, regardless of the position of the reactive residue...
  50. doi Conformational change of Escherichia coli signal recognition particle Ffh is affected by the functionality of signal peptides of ribose-binding protein
    Taeho Ahn
    Department of Biochemistry, College of Veterinary Medicine, Chonnam National University, Gwangju 500 757, Korea
    Mol Cells 27:681-7. 2009
    ..mutant (MT) of ribose-binding protein, on the conformational changes of signal recognition particle 54 homologue (Ffh) in Escherichia coli...
  51. pmc The crystal structure of the signal recognition particle in complex with its receptor
    Sandro F Ataide
    Institute of Molecular Biology and Biophysics, Eidgenössische Technische Hochschule Zurich ETH Zurich, Zurich, Switzerland
    Science 331:881-6. 2011
    ..This rearrangement provides a mechanism for coupling GTP hydrolysis to the handover of cargo to the translocon...
  52. pmc Structure of a GDP:AlF4 complex of the SRP GTPases Ffh and FtsY, and identification of a peripheral nucleotide interaction site
    Pamela J Focia
    Department of Molecular Pharmacology and Biological Chemistry, Feinberg School of Medicine, Northwestern University, 303 E Chicago Ave, Chicago, IL 60611, USA
    J Mol Biol 360:631-43. 2006
    The signal recognition particle (SRP) GTPases Ffh and FtsY play a central role in co-translational targeting of proteins, assembling in a GTP-dependent manner to generate the SRP targeting complex at the membrane...
  53. pmc Ligand crowding at a nascent signal sequence
    Gottfried Eisner
    Institut fur Biochemie und Molekularbiologie, Hermann Herder Strasse 7, D 79104 Freiburg, Germany
    J Cell Biol 163:35-44. 2003
    ..Immediately after emerging from the ribosome, the signal sequence of pOmpA is accessible to Ffh, the protein component of the bacterial signal recognition particle, and to SecA, but it remains attached to the ..
  54. pmc Structure of 4.5S RNA in the signal recognition particle of Escherichia coli as studied by enzymatic and chemical probing
    G Lentzen
    Institut fur Molekularbiologie, Universitat Witten Herdecke, Germany
    RNA 2:244-53. 1996
    ..coli homologue of the signal recognition particle (SRP) RNA, alone and in the SRP complex with protein P48 (Ffh) was probed both enzymatically and chemically...
  55. pmc Functional analysis of the ffh-trmD region of the Escherichia coli chromosome by using reverse genetics
    B C Persson
    Department of Microbiology, University of Umea, Sweden
    J Bacteriol 177:5554-60. 1995
    ..of each of four genes in the Escherichia coli trmD operon (rpsP, 21K, trmD, and rplS) and of the flanking genes ffh and 16K by a reverse genetic method...
  56. ncbi Formation of SRP-like particle induces a conformational change in E. coli 4.5S RNA
    G Lentzen
    Institut fur Molekularbiologie, Universitat Witten Herdecke, Germany
    FEBS Lett 348:233-8. 1994
    ..From equilibrium titrations with fluorescent 4.5S RNA, a dissociation constant of 0.15 microns is obtained for the RNA.protein complex. The formation of the complex is not affected by GTP binding to or hydrolysis by P48...
  57. ncbi The GTPase activity of the Escherichia coli Ffh protein is important for normal growth
    T Samuelsson
    Department of Medical Biochemistry, Goteborg University, Sweden
    Biochim Biophys Acta 1267:83-91. 1995
    The Escherichia coli (E. coli) Ffh protein is homologous to the 54kDa subunit of the eukaryotic signal recognition particle...
  58. ncbi Structure of a methionine-rich segment of Escherichia coli Ffh protein
    D B Oh
    Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Taejon, South Korea
    FEBS Lett 395:160-4. 1996
    The methionine-rich segments of the Ffh protein of Escherichia coli and its eukaryotic counterpart SRP54 are thought to bind signal sequences of secretory proteins...
  59. ncbi Analysis of Escherichia coli 4.5S RNA binding affinity to Ffh and EF-G
    S Suzuma
    Institute of Biological Sciences, University of Tsukuba, Tsukuba shi, Ibaraki, Japan
    FEMS Microbiol Lett 180:271-7. 1999
    Escherichia coli 4.5S RNA is a member of the signal recognition particle RNA family that binds to Ffh and EF-G proteins in vivo. To assess the binding affinity of E. coli 4...
  60. ncbi Decreased requirement for 4.5S RNA in 16S and 23S rRNA mutants of Escherichia coli
    Carleen A Brunelli
    J W Wilson Laboratory, Department of Molecular and Cellular Biology and Biochemistry, Brown University, Providence, RI 02912, USA
    FEBS Lett 514:44-8. 2002
    ..at C732 in 16S rRNA and at A1668 and G1423 in 23S rRNA altered the cellular responses to decreases in both Ffh (the bacterial homolog of SRP54) and 4...
  61. ncbi Evolution of a molecular switch: universal bacterial GTPases regulate ribosome function
    C E Caldon
    School of Microbiology and Immunology, The University of New South Wales, Sydney, Australia
    Mol Microbiol 41:289-97. 2001
    ..of 11 universally conserved GTPases (elongation factor G and Tu, initiation factor 2, LepA, Era, Obg, ThdF/TrmE, Ffh, FtsY, EngA and YchF)...
  62. ncbi Signal recognition particle (SRP)-mediated targeting and Sec-dependent translocation of an extracellular Escherichia coli protein
    Robert Sijbrandi
    Department of Molecular Microbiology, Vrije Universiteit, De Boelelaan 1087, 1081 HV Amsterdam, The Netherlands
    J Biol Chem 278:4654-9. 2003
    ..We propose that these autotransporters preferentially use the co-translational SRP/Sec route to avoid adverse effects of the exposure of their mature domains in the cytoplasm...
  63. ncbi Signal recognition particle mediated protein targeting in Escherichia coli
    Q A Valent
    Department of Molecular Microbiology, Institute of Molecular Biological Sciences, BioCentrum Amsterdam, Faculty of Biology, Vrije Universiteit Amsterdam, The Netherlands
    Antonie Van Leeuwenhoek 79:17-31. 2001
    ..coli SRP and its receptor are discussed. In particular, the interplay between the SRP pathway and other targeting routes, the role of guanine nucleotides in cycling of the SRP and the substrate specificity of the SRP are highlighted...
  64. ncbi Signal peptides bind and aggregate RNA. An alternative explanation for GTPase inhibition in the signal recognition particle
    J F Swain
    Department of Biochemistry and Molecular Biology, University of Massachusetts, Amherst, Massachusetts 01003, USA
    J Biol Chem 276:12222-7. 2001
    ....
  65. ncbi The primary pathway of protein export in E. coli
    P Bassford
    Department of Microbiology and Immunology, University of North Carolina, Chapel Hill 27514
    Cell 65:367-8. 1991
  66. pmc Membrane binding of the bacterial signal recognition particle receptor involves two distinct binding sites
    Sandra Angelini
    Institute for Biochemistry and Molecular Biology, Faculty of Medicine, University of Freiburg, 79104 Freiburg, Germany
    J Cell Biol 174:715-24. 2006
    ..This interaction is stabilized by the N-terminal A-domain of FtsY, which probably serves as a transient lipid anchor...
  67. pmc The signal recognition particle (SRP) RNA links conformational changes in the SRP to protein targeting
    Niels Bradshaw
    Howard Hughes Medical Institute, Department of Biochemistry and Biophysics, University of California at San Francisco, CA 94158, USA
    Mol Biol Cell 18:2728-34. 2007
    ..Using a reverse-genetic and biochemical analysis, we identified mutations in the E. coli SRP protein, Ffh, that abrogate the activity of the SRP RNA and cause corresponding targeting defects in vivo...
  68. ncbi Flexibility in targeting and insertion during bacterial membrane protein biogenesis
    Edwin van Bloois
    Department of Molecular Microbiology, Institute of Molecular Cell Biology, Vrije Universiteit, De Boelelaan 1085, 1081 HV Amsterdam, The Netherlands
    Biochem Biophys Res Commun 362:727-33. 2007
    ..The modified model IMPs were analyzed for altered routing using an in vivo protease mapping approach. The data suggest a facultative use of different combinations of factors...
  69. pmc The nucleotide sequence of an Escherichia coli operon containing genes for the tRNA(m1G)methyltransferase, the ribosomal proteins S16 and L19 and a 21-K polypeptide
    A S Byström
    EMBO J 2:899-905. 1983
    ..e., high preference for the most abundant tRNA isoaccepting species. The trmD gene has a codon usage typical for a protein made in low amount in accordance with the low number of tRNA-(m1G)methyltransferase molecules found in the cell...
  70. ncbi E. coli 4.5S RNA is part of a ribonucleoprotein particle that has properties related to signal recognition particle
    V Ribes
    European Molecular Biology Laboratory, Heidelberg, Federal Republic of Germany
    Cell 63:591-600. 1990
    ..Strains overproducing P48 also accumulate pre-beta-lactamase. 4.5S RNA and P48 are components of a ribonucleoprotein particle that we propose to be required for the secretion of some proteins...
  71. ncbi 4.5S RNA: does form predict function?
    S Brown
    Laboratory of Chromosome Biology, National Cancer Institute Frederick Cancer Research and Development Center, MD 21702
    New Biol 3:430-8. 1991
    ..Surprisingly, no effects on protein secretion in E. coli have been directly attributed to 4.5S RNA. These observations raise the question of whether molecules of similar structure necessarily perform the same function...
  72. doi Subunit a of the F(1)F(0) ATP synthase requires YidC and SecYEG for membrane insertion
    Stefan Kol
    Department of Molecular Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Haren, The Netherlands
    J Mol Biol 390:893-901. 2009
    ..In vitro analysis demonstrates that subunit a of the F(1)F(0) ATP synthase (F(0)a) is strictly dependent on Ffh, SecYEG and YidC for its membrane insertion but independent of the proton motive force...
  73. ncbi The E. coli ffh gene is necessary for viability and efficient protein export
    G J Phillips
    Department of Biology, College of William and Mary, Williamsburg, Virginia 23185
    Nature 359:744-6. 1992
    ..The Escherichia coli homologue, termed ffh (for fifty-four homologue), specifies a protein (Ffh) that shares many properties with its eukaryotic counterpart, ..
  74. pmc Rational design of a fusion partner for membrane protein expression in E. coli
    Jianying Luo
    New England Biolabs, Inc Gene Expression Division, Ipswich, Massachusetts 01938, USA
    Protein Sci 18:1735-44. 2009
    ..We anticipate that this optimized expression system will aid in the isolation and study of various recombinant forms of membrane-associated protein...
  75. ncbi An E. coli ribonucleoprotein containing 4.5S RNA resembles mammalian signal recognition particle
    M A Poritz
    Department of Biochemistry and Biophysics, University of California Medical School, San Francisco 94143 0448
    Science 250:1111-7. 1990
    ..Sequence comparisons, however, have identified (i) a gene in Escherichia coli (ffh) whose product is homologous to the 54-kilodalton subunit (SRP54) of SRP, and (ii) an RNA encoded by the ffs gene (..
  76. ncbi Membrane-associated GTPases in bacteria
    P E March
    Department of Biochemistry, University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School, Piscataway 08854
    Mol Microbiol 6:1253-7. 1992
    ..The amino acid sequences of FtsY and Ffh are clearly related to eukaryotic factors involved in protein secretion...
  77. ncbi Alternate recruitment of signal recognition particle and trigger factor to the signal sequence of a growing nascent polypeptide
    Gottfried Eisner
    Institut fur Biochemie und Molekularbiologie, Universitat Freiburg, Hermann Herder Strasse 7, D 79104 Freiburg, Germany
    J Biol Chem 281:7172-9. 2006
    ..The data also imply that a signal sequence owes its molecular environment to the fact that it remains closely apposed to the ribosomal exit site during growth of a nascent secretory protein...
  78. pmc The tmRDB and SRPDB resources
    Ebbe Sloth Andersen
    Department of Molecular Biology, University of Aarhus, C F Moellers Alle, Building 139, 8000 Aarhus, Denmark
    Nucleic Acids Res 34:D163-8. 2006
    ..S1, alanyl-tRNA synthetase and Elongation Factor Tu, as well as the SRP proteins SRP9, SRP14, SRP19, SRP21, SRP54 (Ffh), SRP68, SRP72, cpSRP43, Flhf, SRP receptor (alpha) and SRP receptor (beta)...
  79. ncbi Development of a minimal cell-free translation system for the synthesis of presecretory and integral membrane proteins
    Yutetsu Kuruma
    Department of Medical Genome Sciences, Graduate School of Frontier Sciences, University of Tokyo, FSB401, 5 1 5 Kashiwanoha, Kashiwa, Chiba 277 8562, Japan
    Biotechnol Prog 21:1243-51. 2005
    ..Next, integration of an inner membrane protein (MtlA) into U-INVs was achieved in the presence of only SRP (Ffh) and SR (FtsY)...
  80. ncbi Interaction of E. coli Ffh/4.5S ribonucleoprotein and FtsY mimics that of mammalian signal recognition particle and its receptor
    J D Miller
    Department of Biochemistry and Biophysics, University of California Medical School, San Francisco 94143 0448
    Nature 367:657-9. 1994
    ..Two proteins from Escherichia coli, Ffh and FTsY, structurally resemble SRP54 and SR alpha...
  81. pmc Peculiar properties of DsbA in its export across the Escherichia coli cytoplasmic membrane
    Nobuyuki Shimohata
    Institute for Virus Research, Kyoto University, Kyoto 606 8507, Japan
    J Bacteriol 187:3997-4004. 2005
    ..DsbA export depended on the SecY translocon, the SecA ATPase, and Ffh (signal recognition particle), but not on SecB...
  82. pmc Domain rearrangement of SRP protein Ffh upon binding 4.5S RNA and the SRP receptor FtsY
    Iwona Buskiewicz
    Institute of Molecular Biology, University of Witten Herdecke, Germany
    RNA 11:947-57. 2005
    ..In Escherichia coli, SRP consists of 4.5S RNA and a protein, Ffh, that recognizes the signal peptide emerging from the ribosome and the SRP receptor at the membrane, FtsY...
  83. pmc Functional substitution of the signal recognition particle 54-kDa subunit by its Escherichia coli homolog
    H D Bernstein
    Department of Biochemistry and Biophysics, University of California Medical School, San Francisco 94143 0448
    Proc Natl Acad Sci U S A 90:5229-33. 1993
    ..J. & Silhavy, T. J. (1992) Nature (London) 359, 744-746] have shown that depletion of this protein, designated Ffh (fifty-four homolog), leads to a significant secretory defect in vivo...
  84. pmc Unraveling the interface of signal recognition particle and its receptor by using chemical cross-linking and tandem mass spectrometry
    Feixia Chu
    Mass Spectrometry Facility, University of California, San Francisco 94143 0046, USA
    Proc Natl Acad Sci U S A 101:16454-9. 2004
    ..particle (SRP)-dependent targeting of proteins to the bacterial plasma membrane, the specific interaction between Ffh (the protein component of SRP) and FtsY (the SRP receptor) is known to be essential for the efficiency and fidelity ..
  85. ncbi Sec/SRP requirements and energetics of membrane insertion of subunits a, b, and c of the Escherichia coli F1F0 ATP synthase
    Liang Yi
    Department of Chemistry, The Ohio State University, Columbus, Ohio 43210, USA
    J Biol Chem 279:39260-7. 2004
    ..Taken together, the in vivo data suggest that subunits a and b are inserted by the Sec/SRP pathway with the help of YidC, and subunit c is integrated into the membrane by the novel YidC pathway...
  86. pmc Methionine sulfoxide reductases protect Ffh from oxidative damages in Escherichia coli
    Benjamin Ezraty
    Laboratoire de Chimie Bacterienne, Institut Fédératif de Recherche Biologie Structurale et Microbiologie, Centre National de la Recherche Scientifique, Marseille Cedex, France
    EMBO J 23:1868-77. 2004
    ..Methionine oxidation is reversed by methionine sulfoxide reductases A and B, a class of highly conserved enzymes. Ffh protein, a component of the ubiquitous signal recognition particle, contains a methionine-rich domain, interacting ..
  87. pmc Important role of the tetraloop region of 4.5S RNA in SRP binding to its receptor FtsY
    J R Jagath
    Institute of Molecular Biology, University of Witten Herdecke, Witten, Germany
    RNA 7:293-301. 2001
    ..by the structure of the tetraloop region of SRP RNA, in particular the tetraloop stem, and suggest that both SRP RNA and Ffh undergo mutual structural adaptation to form SRP that is functional in the interaction with the receptor, FtsY.
  88. ncbi Structure of the E. coli signal recognition particle bound to a translating ribosome
    Christiane Schaffitzel
    ETH Zurich, Institute for Molecular Biology and Biophysics, HPK Building, Schafmattstrasse 20, 8093 Zurich, Switzerland
    Nature 444:503-6. 2006
    ....
  89. pmc Conformation of 4.5S RNA in the signal recognition particle and on the 30S ribosomal subunit
    Shan Qing Gu
    Institute of Molecular Biology, University of Witten Herdecke, Stockumer Str 10, 58448 Witten, Germany
    RNA 11:1374-84. 2005
    The signal recognition particle (SRP) from Escherichia coli consists of 4.5S RNA and protein Ffh. It is essential for targeting ribosomes that are translating integral membrane proteins to the translocation pore in the plasma membrane...
  90. ncbi Conformations of the signal recognition particle protein Ffh from Escherichia coli as determined by FRET
    Iwona Buskiewicz
    Institute of Molecular Biology, University of Witten Herdecke, 58448 Witten, Germany
    J Mol Biol 351:417-30. 2005
    ..SRP in Escherichia coli is composed of one protein, Ffh, and 4.5S RNA. In the present work, we probe the structure of Ffh alone and in the complex with 4...
  91. pmc Conserved but nonessential interaction of SRP RNA with translation factor EF-G
    Madi Bidya Sagar
    Department of Molecular Biophysics and Biochemistry, Howard Hughes Medical Institute, Yale University, New Haven, CT 06520, USA
    RNA 10:772-8. 2004
    ..5S binding to its cognate SRP protein, Ffh. Mutational analysis indicates that the same molecular structure of 4.5S RNA is recognized in each case...