eno

Summary

Gene Symbol: eno
Description: enolase
Alias: ECK2773, JW2750
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. pmc Characterization of the RNA degradosome of Pseudoalteromonas haloplanktis: conservation of the RNase E-RhlB interaction in the gammaproteobacteria
    Soraya Aït-Bara
    Laboratoire de Microbiologie et Genetique Moleculaires, UMR 5100, Centre National de la Recherche Scientifique et Université de Toulouse 3, 31062 Toulouse, France
    J Bacteriol 192:5413-23. 2010
  2. doi Cell cycle progression in Escherichia coli B/r affects transcription of certain genes: Implications for synthetic genome design
    Patricia L Echtenkamp
    School of Chemical and Biomolecular Engineering, Cornell University, Ithaca, New York 14853 5201, USA
    Biotechnol Bioeng 102:902-9. 2009
  3. ncbi Analysis of the Escherichia coli RNA degradosome composition by a proteomic approach
    Maria Elena Regonesi
    Dipartimento di Scienze Biomolecolari e Biotecnologie, Universita degli Studi di Milano, Via Celoria 26, 20133 Milan, Italy
    Biochimie 88:151-61. 2006
  4. pmc ColE1 hybrid plasmids for Escherichia coli genes of glycolysis and the hexose monophosphate shunt
    J Thomson
    J Bacteriol 137:502-6. 1979
  5. ncbi Function in Escherichia coli of the non-catalytic part of RNase E: role in the degradation of ribosome-free mRNA
    Anne Leroy
    Laboratoire de Microbiologie et Génétique Moléculaire CNRS, UMR 5100, Universite Paul Sabatier, 118 rue de Narbonne, 31062 Toulouse, France
    Mol Microbiol 45:1231-43. 2002
  6. ncbi The RNA degradosome and poly(A) polymerase of Escherichia coli are required in vivo for the degradation of small mRNA decay intermediates containing REP-stabilizers
    Vanessa Khemici
    Laboratoire de Microbiologie et Genetique Moleculaire, CNRS, UMR 5100 and Paul Sabatier Université, 118 route de Narbonne, 31062 Toulouse, France
    Mol Microbiol 51:777-90. 2004
  7. pmc Global analysis of Escherichia coli RNA degradosome function using DNA microarrays
    Jonathan A Bernstein
    Department of Genetics, Stanford University, Stanford, CA 94305, USA
    Proc Natl Acad Sci U S A 101:2758-63. 2004
  8. ncbi Studies of the RNA degradosome-organizing domain of the Escherichia coli ribonuclease RNase E
    Anastasia J Callaghan
    Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Cambridge CB2 1GA, UK
    J Mol Biol 340:965-79. 2004
  9. ncbi Enolase in the RNA degradosome plays a crucial role in the rapid decay of glucose transporter mRNA in the response to phosphosugar stress in Escherichia coli
    Teppei Morita
    Division of Biological Science, Graduate School of Science, Nagoya University, Chikusa, Nagoya 464 8602, Japan
    Mol Microbiol 54:1063-75. 2004
  10. ncbi Physical and functional interactions among RNase E, polynucleotide phosphorylase and the cold-shock protein, CsdA: evidence for a 'cold shock degradosome'
    Annie Prud'homme-Généreux
    Department of Biochemistry and Molecular Biology, University of British Columbia, Vancouver, BC, V6T 1Z3, Canada
    Mol Microbiol 54:1409-21. 2004

Research Grants

Scientific Experts

  • JOHN GERLT
  • A J Carpousis
  • Carin K Vanderpool
  • Vladimir R Kaberdin
  • Ben F Luisi
  • Vidya Chandran
  • Vanessa Khemici
  • J Allen Easton
  • Pei Hsun Lin
  • S Lin-Chao
  • E Blum
  • Salima Nurmohamed
  • Soraya Aït-Bara
  • Patricia L Echtenkamp
  • Stefanie S Jourdan
  • B Py
  • Carol V Robinson
  • G A Coburn
  • Aziz Taghbalout
  • C F Higgins
  • Maria Jose Marcaida
  • Peter Thompson
  • Maria Elena Regonesi
  • Michael W Crowder
  • Tara K Sigdel
  • Tomohiro Shimada
  • Michael J Kerner
  • G G Liou
  • Sue Lin-Chao
  • Gregory Boel
  • Leonora Poljak
  • Teppei Morita
  • Jonathan A Bernstein
  • Anastasia J Callaghan
  • Annie Prud'homme-Généreux
  • Nathalie F Vanzo
  • Russell R Poyner
  • Anne Leroy
  • C L Wei
  • K Kühnel
  • S N Cohen
  • Y Feng
  • Adam R McKay
  • Michael L Shuler
  • David B Wilson
  • Kenneth J McDowall
  • G A Mackie
  • Lawrence Rothfield
  • Renee Cilliers
  • D A Bessarab
  • N F Vanzo
  • Priya R Gursahaney
  • Paolo Tortora
  • Federica Briani
  • Fabrizio Basilico
  • Louise Benazzi
  • Gianni Dehò
  • A Rompf
  • Marta Del Favero
  • Pierluigi Mauri
  • Mark A DePristo
  • Tobias Maier
  • Hung chun Chang
  • Akira Ishihama
  • Manajit Hayer-Hartl
  • F Ulrich Hartl
  • Nobuyuki Fujita
  • Matthias Mann
  • Dmitrij Frishman
  • Anna P Stines
  • Yasushi Ishihama
  • Dean J Naylor
  • Michihisa Maeda
  • Costa Georgopoulos
  • Leopold L Ilag
  • Stanley N Cohen
  • H M Krisch
  • Toshifumi Inada
  • Axel Hartke
  • Yanick Auffray
  • Ivan Mijakovic
  • Jukka P Aurikko
  • Karin Kühnel
  • George A Mackie
  • Hiroji Aiba
  • Josef Deutscher
  • Isabelle Toesca
  • Isabelle Iost
  • Taisei Mizota
  • C Shane Ramey

Detail Information

Publications49

  1. pmc Characterization of the RNA degradosome of Pseudoalteromonas haloplanktis: conservation of the RNase E-RhlB interaction in the gammaproteobacteria
    Soraya Aït-Bara
    Laboratoire de Microbiologie et Genetique Moleculaires, UMR 5100, Centre National de la Recherche Scientifique et Université de Toulouse 3, 31062 Toulouse, France
    J Bacteriol 192:5413-23. 2010
    ..Since the activity of Ec-RhlB requires its physical interaction with Ec-RNase E, conservation of the underlying structural motif over a large evolutionary distance could be due to constraints involved in the control of RhlB activity...
  2. doi Cell cycle progression in Escherichia coli B/r affects transcription of certain genes: Implications for synthetic genome design
    Patricia L Echtenkamp
    School of Chemical and Biomolecular Engineering, Cornell University, Ithaca, New York 14853 5201, USA
    Biotechnol Bioeng 102:902-9. 2009
    ..In conclusion, gene position, with regard to the C period, and gene function are important factors to incorporate into design criteria for synthetic bacterial genomes...
  3. ncbi Analysis of the Escherichia coli RNA degradosome composition by a proteomic approach
    Maria Elena Regonesi
    Dipartimento di Scienze Biomolecolari e Biotecnologie, Universita degli Studi di Milano, Via Celoria 26, 20133 Milan, Italy
    Biochimie 88:151-61. 2006
    ..In addition, we show that normalized score values obtain by MudPIT analysis may be taken as quantitative estimates of the relative protein abundance in different degradosome preparations...
  4. pmc ColE1 hybrid plasmids for Escherichia coli genes of glycolysis and the hexose monophosphate shunt
    J Thomson
    J Bacteriol 137:502-6. 1979
    ..isomerase (pgi), glucose-6-phosphate dehydrogenase (zwf), gluconate-6-phosphate dehydrogenase (gnd), enolase (eno), phosphoglycerate kinase (pgk), and fructose-1,6-P2 aldolase (fda)...
  5. ncbi Function in Escherichia coli of the non-catalytic part of RNase E: role in the degradation of ribosome-free mRNA
    Anne Leroy
    Laboratoire de Microbiologie et Génétique Moléculaire CNRS, UMR 5100, Universite Paul Sabatier, 118 rue de Narbonne, 31062 Toulouse, France
    Mol Microbiol 45:1231-43. 2002
    ..Although the catalytic domain of RNase E is sufficient for viability, our work demonstrates that elements in the non-catalytic part are necessary for normal activity in vivo...
  6. ncbi The RNA degradosome and poly(A) polymerase of Escherichia coli are required in vivo for the degradation of small mRNA decay intermediates containing REP-stabilizers
    Vanessa Khemici
    Laboratoire de Microbiologie et Genetique Moleculaire, CNRS, UMR 5100 and Paul Sabatier Université, 118 route de Narbonne, 31062 Toulouse, France
    Mol Microbiol 51:777-90. 2004
    ....
  7. pmc Global analysis of Escherichia coli RNA degradosome function using DNA microarrays
    Jonathan A Bernstein
    Department of Genetics, Stanford University, Stanford, CA 94305, USA
    Proc Natl Acad Sci U S A 101:2758-63. 2004
    ....
  8. ncbi Studies of the RNA degradosome-organizing domain of the Escherichia coli ribonuclease RNase E
    Anastasia J Callaghan
    Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Cambridge CB2 1GA, UK
    J Mol Biol 340:965-79. 2004
    ..The carboxy-terminal domain of RNase E may thus act as a flexible tether of the degradosome components. The implications of these and other observations for the organization of the RNA degradosome are discussed...
  9. ncbi Enolase in the RNA degradosome plays a crucial role in the rapid decay of glucose transporter mRNA in the response to phosphosugar stress in Escherichia coli
    Teppei Morita
    Division of Biological Science, Graduate School of Science, Nagoya University, Chikusa, Nagoya 464 8602, Japan
    Mol Microbiol 54:1063-75. 2004
    ..In addition, we show that PNPase and RhlB within the degradosome cooperate to eliminate short degradation intermediates of ptsG mRNA...
  10. ncbi Physical and functional interactions among RNase E, polynucleotide phosphorylase and the cold-shock protein, CsdA: evidence for a 'cold shock degradosome'
    Annie Prud'homme-Généreux
    Department of Biochemistry and Molecular Biology, University of British Columbia, Vancouver, BC, V6T 1Z3, Canada
    Mol Microbiol 54:1409-21. 2004
    ..These findings show that the RNA degradosome is a flexible macromolecular machine capable of adapting to altered environmental conditions...
  11. ncbi The RNase E of Escherichia coli has at least two binding sites for DEAD-box RNA helicases: functional replacement of RhlB by RhlE
    Vanessa Khemici
    Laboratoire de Microbiologie et Genetique Moleculaires, UMR 5100, Centre National de la Recherche Scientifique CNRS et Université Paul Sabatier, 118 route de Narbonne, 31062 Toulouse, France
    Mol Microbiol 54:1422-30. 2004
    ..Thus, RhlE can replace the function of RhlB in vitro. The results in the accompanying article show that CsdA can also replace RhlB in vitro. Thus, RhlB, RhlE and CsdA are interchangeable in in vitro RNA degradation assays...
  12. ncbi Divergent evolution in the enolase superfamily: the interplay of mechanism and specificity
    John A Gerlt
    Departments of Biochemistry and Chemistry, University of Illinois, Urbana, IL 61801, USA
    Arch Biochem Biophys 433:59-70. 2005
    ..In this minireview, our current understanding of structure/function relationships in the divergent members of the superfamily is reviewed, and the use of this knowledge for our future studies is proposed...
  13. ncbi Proteome-wide analysis of chaperonin-dependent protein folding in Escherichia coli
    Michael J Kerner
    Department of Cellular Biochemistry, Max Planck Institute of Biochemistry, Am Klopferspitz 18, D 82152 Martinsried, Germany
    Cell 122:209-20. 2005
    ..We suggest that the chaperonin system may have facilitated the evolution of this fold into a versatile platform for the implementation of numerous enzymatic functions...
  14. ncbi Systematic search for the Cra-binding promoters using genomic SELEX system
    Tomohiro Shimada
    Nippon Institute for Biological Science, Division of Molecular Biology, Ome, Tokyo 198 0024, Japan
    Genes Cells 10:907-18. 2005
    ..sequences, two are associated with promoter regions of the gapA (glyceraldehyde 3-phosphate dehydrogenase) and eno (enolase) genes, both involved in sugar metabolism...
  15. ncbi Functional and structural changes due to a serine to alanine mutation in the active-site flap of enolase
    Russell R Poyner
    Department of Biochemistry, University of Wisconsin Madison, Madison, WI 53705, USA
    Arch Biochem Biophys 401:155-63. 2002
    ..7 A from the closest phosphonate oxygen, and the loop (154-166) containing His 159 is shifted away from the active center. A peripheral loop, Glu 251-Gly 275, also moves to open access to the active site...
  16. pmc RhlB helicase rather than enolase is the beta-subunit of the Escherichia coli polynucleotide phosphorylase (PNPase)-exoribonucleolytic complex
    Pei Hsun Lin
    Institute of Molecular Biology, Academia Sinica, Taipei 115, Taiwan
    Proc Natl Acad Sci U S A 102:16590-5. 2005
    ..e., 377-380 kDa), whereas no association between PNPase-alpha and enolase was detected. Chromosomal deletion of the eno gene had no effect on the ability of PNPase to degrade either single- or double-stranded RNAs...
  17. ncbi Recognition of enolase in the Escherichia coli RNA degradosome
    Vidya Chandran
    Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Cambridge CB2 1GA, UK
    J Mol Biol 358:8-15. 2006
    ..We suggest that enolase is recruited into putative RNA degradosome machinery in these bacilli, where it plays common regulatory functions...
  18. ncbi The RNA degradosome: life in the fast lane of adaptive molecular evolution
    Maria Jose Marcaida
    Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Cambridge CB2 1GA, UK
    Trends Biochem Sci 31:359-65. 2006
    ....
  19. ncbi Probing the adaptive response of Escherichia coli to extracellular Zn(II)
    Tara K Sigdel
    Department of Chemistry and Biochemistry, Miami University, 112 Hughes Hall, Oxford, OH 45056, USA
    Biometals 19:461-71. 2006
    ..coli proteins, and the results are discussed in light of recent genomic profiling studies on the adaptive response of E. coli cells to stress by Zn(II) excess...
  20. pmc RNaseE and the other constituents of the RNA degradosome are components of the bacterial cytoskeleton
    Aziz Taghbalout
    Department of Molecular, Microbial, and Structural Biology, University of Connecticut Health Center, Farmington, CT 06032, USA
    Proc Natl Acad Sci U S A 104:1667-72. 2007
    ....
  21. ncbi Physiological consequences of small RNA-mediated regulation of glucose-phosphate stress
    Carin K Vanderpool
    Department of Microbiology, University of Illinois at Urbana Champaign B213, Chemical and Life Sciences Laboratories CLSL, MC 110 Urbana, IL 61801, USA
    Curr Opin Microbiol 10:146-51. 2007
    ..The current model is that SgrS promotes recovery by stopping the synthesis of glucose transport proteins, which in turn limits the accumulation of toxic sugar-phosphates...
  22. ncbi Sensing of 5' monophosphate by Escherichia coli RNase G can significantly enhance association with RNA and stimulate the decay of functional mRNA transcripts in vivo
    Stefanie S Jourdan
    Astbury Centre for Structural Molecular Biology, Faculty of Biological Sciences, University of Leeds, England, UK
    Mol Microbiol 67:102-15. 2008
    ....
  23. ncbi Unraveling new roles for minor components of the E. coli RNA degradosome
    Vladimir R Kaberdin
    Institute of Molecular Biology, Academia Sinica, Nankang, Taipei, Taiwan
    RNA Biol 6:402-5. 2009
    ..Here we briefly review these findings and discuss their implications for understanding the multifaceted mechanisms controlling degradosome functions in vivo...
  24. pmc Molecular recognition between Escherichia coli enolase and ribonuclease E
    Salima Nurmohamed
    Department of Biochemistry, University of Cambridge, England
    Acta Crystallogr D Biol Crystallogr 66:1036-40. 2010
    ..9 A resolution is presented. The structure suggests that enolase may help to organize an adjacent conserved RNA-binding motif in RNase E...
  25. ncbi Polyphosphate kinase is a component of the Escherichia coli RNA degradosome
    E Blum
    Nuffield Department of Clinical Biochemistry, Institute of Molecular Medicine, John Radcliffe Hospital, University of Oxford, UK
    Mol Microbiol 26:387-98. 1997
    ..Thus, PPK in the degradosome appears to maintain an appropriate microenvironment, removing inhibitory polyphosphate and NDPs and regenerating ATP...
  26. pmc Escherichia coli mutant containing a large deletion from relA to argA
    A G Atherly
    J Bacteriol 138:530-4. 1979
    ..Further analysis demonstrated loss of relA, fuc, and argA gene functions but retention of eno and recB, closely linked genes adjacent to relA and argA, respectively...
  27. ncbi Nucleotide sequence of Escherichia coli pyrG encoding CTP synthetase
    M Weng
    J Biol Chem 261:5568-74. 1986
    ..Translation of the gene eno, encoding enolase, is initiated 89 base pairs downstream of pyrG...
  28. ncbi The purification and characterization of Escherichia coli enolase
    T G Spring
    J Biol Chem 246:6797-802. 1971
  29. ncbi Copurification of E. coli RNAase E and PNPase: evidence for a specific association between two enzymes important in RNA processing and degradation
    A J Carpousis
    Department of Molecular Biology, University of Geneva, Switzerland
    Cell 76:889-900. 1994
    ..The discovery of a specific association between RNAase E and PNPase raises the intriguing possibility that these enzymes act cooperatively in the processing and degradation of RNA...
  30. ncbi A protein complex mediating mRNA degradation in Escherichia coli
    B Py
    Imperial Cancer Research Fund Laboratories, Institute of Molecular Medicine, Oxford, UK
    Mol Microbiol 14:717-29. 1994
    ..The identification of a complex which includes several activities associated with mRNA degradation has implications for the mechanisms and co-ordinated control of mRNA degradation...
  31. ncbi A DEAD-box RNA helicase in the Escherichia coli RNA degradosome
    B Py
    Nuffield Department of Clinical Biochemisstry and Imperial Cancer Research Fund Laboratories, Institute of Molecular Medicine, UK
    Nature 381:169-72. 1996
    ..These results suggest that RhlB acts by unwinding RNA structures that impede the processive activity of PNPase. RhlB is thus an important enzyme in mRNA turnover...
  32. pmc Proteins associated with RNase E in a multicomponent ribonucleolytic complex
    A Miczak
    Institute of Molecular Biology, Academia Sinica, Nankang Taipei, Taiwan, Republic of China
    Proc Natl Acad Sci U S A 93:3865-9. 1996
    ..The FLAG-Rne complex has RNase E activity in vivo and in vitro. The relative amount of proteins associated with wild-type and Rne-3071 expressed at an elevated temperature differed...
  33. pmc RNase E polypeptides lacking a carboxyl-terminal half suppress a mukB mutation in Escherichia coli
    M Kido
    Department of Molecular Cell Biology, Institute of Molecular Embryology and Genetics, Kumamoto University School of Medicine, Kumamoto, Japan
    J Bacteriol 178:3917-25. 1996
    ..RNase E and PNPase of the multiprotein complex presumably cooperate for effective processing and turnover of specific substrates, such as mRNAs and other RNAs in vivo...
  34. ncbi Cloning, nucleotide sequence, and functional expression of the Escherichia coli enolase (eno) gene in a temperature-sensitive eno mutant strain
    M Klein
    Institut für Biotechnologie 1, Forschungszentrum Julich GmbH, Germany
    DNA Seq 6:351-5. 1996
    The entire Escherichia coli eno gene was cloned by functional complementation of a newly isolated temperature-sensitive enolase mutant and its nucleotide sequence determined...
  35. ncbi The Escherichia coli RNA degradosome: structure, function and relationship in other ribonucleolytic multienzyme complexes
    A J Carpousis
    Laboratoire de Microbiologie et Genetique Moleculaire, Centre National de la Recherche Scientifique, UMR 5100, 118 route de Narbonne, 31061 Toulouse Cedex, France
    Biochem Soc Trans 30:150-5. 2002
    ..RhlB in vitro can facilitate the degradation of structured RNA by PNPase. Since the discovery of the RNA degradosome in E. coli, related complexes have been described in other organisms...
  36. pmc RNA components of Escherichia coli degradosome: evidence for rRNA decay
    D A Bessarab
    Institute of Molecular Biology, Academia Sinica, Nankang, Taipei 115, Taiwan, Republic of China
    Proc Natl Acad Sci U S A 95:3157-61. 1998
    ..Our results, which provide evidence that decay of mature rRNAs occurs in growing Escherichia coli cells in the RNA degradosome, implicate RNase E in degradosome-mediated decay...
  37. ncbi Reconstitution of the degradation of the mRNA for ribosomal protein S20 with purified enzymes
    G A Coburn
    D H Copp Building, University of British Columbia, 2146 Health Sciences Mall, Vancouver, British Columbia, V6T 1Z3, Canada
    J Mol Biol 279:1061-74. 1998
    ..Moreover, decay of a model mRNA can be reconstituted in vitro by a small number of purified components in a process which is more dynamic and ATP-dependent than previously imagined...
  38. pmc Ribonuclease E organizes the protein interactions in the Escherichia coli RNA degradosome
    N F Vanzo
    Laboratoire de Microbiologie et Genetique Moleculaire, UPR 9007, Centre National de la Recherche Scientifique CNRS, 31062 Toulouse Cedex, France
    Genes Dev 12:2770-81. 1998
    ....
  39. ncbi Changes in protein synthesis as a consequence of heme depletion in Escherichia coli
    A Rompf
    Institut fur Organische Chemie und Biochemie, Albert Ludwigs Universitat Freiburg, Albertstr 21, 79104 Freiburg i Br, Germany
    Curr Microbiol 37:226-30. 1998
    ..of tryptophanase (trpA), citrate synthase (gltA), and aldehyde dehydrogenase (aldA) and the repression of enolase (eno) and phosphoglycerate kinase (pgk) were observed...
  40. ncbi Polyadenylation promotes degradation of 3'-structured RNA by the Escherichia coli mRNA degradosome in vitro
    E Blum
    Nuffield Department of Clinical Biochemistry, Institute of Molecular Medicine, John Radcliffe Hospital, University of Oxford, Oxford OX3 9DS, United Kingdom
    J Biol Chem 274:4009-16. 1999
    ..The inaccessibility of 3'-oligo(U) sequences is likely to have a role in stabilization of RNA molecules generated by Rho-independent terminators...
  41. pmc Reconstitution of a minimal RNA degradosome demonstrates functional coordination between a 3' exonuclease and a DEAD-box RNA helicase
    G A Coburn
    Department of Biochemistry and Molecular Biology, University of British Columbia, Vancouver, British Columbia V6T 1Z3 Canada
    Genes Dev 13:2594-603. 1999
    ..A model for degradosome-mediated degradation of structured RNA is presented with its implications for mRNA decay in Escherichia coli...
  42. pmc RNase E is required for the maturation of ssrA RNA and normal ssrA RNA peptide-tagging activity
    S Lin-Chao
    Institute of Molecular Biology, Academia Sinica, Taipei 115, Taiwan
    Proc Natl Acad Sci U S A 96:12406-11. 1999
    ..coli, also has the previously unsuspected ability to affect protein degradation through its role in maturation of the 3' end of ssrA RNA...
  43. pmc RNA degradosomes exist in vivo in Escherichia coli as multicomponent complexes associated with the cytoplasmic membrane via the N-terminal region of ribonuclease E
    G G Liou
    Institute of Molecular Biology, Academia Sinica, Nankang, Taipei 115, Taiwan
    Proc Natl Acad Sci U S A 98:63-8. 2001
    ..Our findings, which establish the existence and cellular location of RNase E-based degradosomes in vivo in E. coli, also suggest that RNA processing and decay may occur at specific sites within cells...
  44. ncbi Escherichia coli poly(A)-binding proteins that interact with components of degradosomes or impede RNA decay mediated by polynucleotide phosphorylase and RNase E
    Y Feng
    Department of Genetics and the Program in Cancer Biology, Stanford University School of Medicine, Stanford, California 94305 5120, USA
    J Biol Chem 276:31651-6. 2001
    ..Our findings raise the prospect that E. coli proteins that bind to poly(A) tails may link the functions of degradosomes and ribosomes...
  45. ncbi Crystal structure of the Escherichia coli RNA degradosome component enolase
    K Kühnel
    Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Cambridge CB2 1GA, UK
    J Mol Biol 313:583-92. 2001
    ..The putative role of enolase in the RNA degradosome is discussed; although it was not possible to ascribe a specific role to it, a structural role is possible...
  46. ncbi Is 2-phosphoglycerate-dependent automodification of bacterial enolases implicated in their export?
    Gregory Boel
    Microbiologie et Genetique Moleculaire, CNRS INRA INA PG, UMR 2585, F 78850, Thiverval Grignon, France
    J Mol Biol 337:485-96. 2004
    ..The K341E mutant enolase was almost as active as the wild-type enzyme and therefore allowed us to establish that the loss of enolase export correlates with the loss of modification and not the loss of glycolytic activity...
  47. ncbi Phosphorylation of Escherichia coli enolase
    H K Dannelly
    Department of Microbiology, Arizona State University, Tempe 85287
    Biochimie 71:1095-100. 1989
    ..Complete reversal of the changes in activity was possible by adding an aliquot of partially purified enolase kinase plus ATP...
  48. pmc Time-dependent translational response of E. coli to excess Zn(II)
    J Allen Easton
    Department of Chemistry and Biochemistry, 160 Hughes Hall, Miami University, Oxford, OH 45056, USA
    J Biomol Tech 17:303-7. 2006
    ..Through the use of two-dimensional gel electrophoresis, we were able to show that the levels of OmpF, AspC, YcdO, Eno, and CysE increased after 30 min of Zn(II) stress, while the levels of Tig, TufA, SelA, and LeuC decreased relative ..

Research Grants2

  1. GENOMIC ENZYMOLOGY: THE CROTONASE SUPERFAMILY
    JOHN GERLT; Fiscal Year: 2003
    ..5) 3-OH Isobutyryl CoA hydrolase (HIBCH) catalyzes hydrolysis of a thioester in valine catabolism. We will study HIBCH so that we can determine whether the reaction proceeds via and enolate anion or an anionic tetrahedral intermediate. ..
  2. GENOMIC ENZYMOLOGY: THE ENOLASE SUPERFAMILY
    JOHN GERLT; Fiscal Year: 2006
    ..4) We will test a structural blueprint for functional diversity in the (beta/alpha)7beta-barrel fold by determining whether new functions can be generated by in vitro evolution. ..