dnaK

Summary

Gene Symbol: dnaK
Description: chaperone Hsp70, with co-chaperone DnaJ
Alias: ECK0014, JW0013, groPAB, groPC, groPF, grpC, grpF, seg
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. pmc An ATPase domain common to prokaryotic cell cycle proteins, sugar kinases, actin, and hsp70 heat shock proteins
    P Bork
    European Molecular Biology Laboratory, Heidelberg, Federal Republic of Germany
    Proc Natl Acad Sci U S A 89:7290-4. 1992
  2. ncbi Physical interaction between heat shock proteins DnaK, DnaJ, and GrpE and the bacterial heat shock transcription factor sigma 32
    J Gamer
    Zentrum fur Molekulare Biologie, Universitat Heidelberg, Germany
    Cell 69:833-42. 1992
  3. pmc Mutations altering heat shock specific subunit of RNA polymerase suppress major cellular defects of E. coli mutants lacking the DnaK chaperone
    B Bukau
    Biology Department, Massachusetts Institute of Technology, Cambridge 02139
    EMBO J 9:4027-36. 1990
  4. pmc Induction of heat shock proteins by abnormal proteins results from stabilization and not increased synthesis of sigma 32 in Escherichia coli
    M Kanemori
    Institute for Virus Research, Kyoto University, Japan
    J Bacteriol 176:5648-53. 1994
  5. pmc Mutant DnaK chaperones cause ribosome assembly defects in Escherichia coli
    J H Alix
    Institut de Biologie Physico Chimique, Paris, France
    Proc Natl Acad Sci U S A 90:9725-9. 1993
  6. pmc A cycle of binding and release of the DnaK, DnaJ and GrpE chaperones regulates activity of the Escherichia coli heat shock transcription factor sigma32
    J Gamer
    Zentrum fur Molekulare Biologie, Universitat Heidelberg, Germany
    EMBO J 15:607-17. 1996
  7. ncbi Regulatory region C of the E. coli heat shock transcription factor, sigma32, constitutes a DnaK binding site and is conserved among eubacteria
    J S McCarty
    Zentrum fur Molekulare Biologie Heidelberg Universitat Heidelberg, FRG
    J Mol Biol 256:829-37. 1996
  8. ncbi Structural analysis of substrate binding by the molecular chaperone DnaK
    X Zhu
    Department of Biochemistry and Molecular Biophysics, College of Physicians and Surgeons, Columbia University, New York 10032, USA
    Science 272:1606-14. 1996
  9. ncbi The Hsp70 and Hsp60 chaperone machines
    B Bukau
    Institut für Biochemie and Molekularbiologie, Universitat Freiburg, Germany
    Cell 92:351-66. 1998
  10. ncbi ClpB cooperates with DnaK, DnaJ, and GrpE in suppressing protein aggregation. A novel multi-chaperone system from Escherichia coli
    M Zolkiewski
    Department of Biochemistry, Kansas State University, Manhattan, Kansas 66506, USA
    J Biol Chem 274:28083-6. 1999

Scientific Experts

Detail Information

Publications120 found, 100 shown here

  1. pmc An ATPase domain common to prokaryotic cell cycle proteins, sugar kinases, actin, and hsp70 heat shock proteins
    P Bork
    European Molecular Biology Laboratory, Heidelberg, Federal Republic of Germany
    Proc Natl Acad Sci U S A 89:7290-4. 1992
    ..A common evolutionary origin for all of the proteins in this class is proposed...
  2. ncbi Physical interaction between heat shock proteins DnaK, DnaJ, and GrpE and the bacterial heat shock transcription factor sigma 32
    J Gamer
    Zentrum fur Molekulare Biologie, Universitat Heidelberg, Germany
    Cell 69:833-42. 1992
    ..This regulatory function has been postulated for Escherichia coli to rely on the direct association of DnaK (Hsp70) with the heat shock transcription factor sigma 32...
  3. pmc Mutations altering heat shock specific subunit of RNA polymerase suppress major cellular defects of E. coli mutants lacking the DnaK chaperone
    B Bukau
    Biology Department, Massachusetts Institute of Technology, Cambridge 02139
    EMBO J 9:4027-36. 1990
    ..In an effort to understand the roles of molecular chaperones such as DnaK in cellular metabolism, we analyzed secondary mutations (sid) that suppress the growth defects of delta dnaK52 ..
  4. pmc Induction of heat shock proteins by abnormal proteins results from stabilization and not increased synthesis of sigma 32 in Escherichia coli
    M Kanemori
    Institute for Virus Research, Kyoto University, Japan
    J Bacteriol 176:5648-53. 1994
    ..Evidence further suggests that the DnaK chaperone plays a crucial regulatory role in induction of the heat shock response by abnormal proteins.
  5. pmc Mutant DnaK chaperones cause ribosome assembly defects in Escherichia coli
    J H Alix
    Institut de Biologie Physico Chimique, Paris, France
    Proc Natl Acad Sci U S A 90:9725-9. 1993
    ..the participation of additional factors, we have studied the influence of a chaperone, the product of the gene dnaK, on ribosome assembly in vivo...
  6. pmc A cycle of binding and release of the DnaK, DnaJ and GrpE chaperones regulates activity of the Escherichia coli heat shock transcription factor sigma32
    J Gamer
    Zentrum fur Molekulare Biologie, Universitat Heidelberg, Germany
    EMBO J 15:607-17. 1996
    The chaperone system formed by DnaK, DnaJ and GrpE mediates stress-dependent negative modulation of the Escherichia coli heat shock response, probably through association with the heat shock promoter-specific sigma32 subunit of RNA ..
  7. ncbi Regulatory region C of the E. coli heat shock transcription factor, sigma32, constitutes a DnaK binding site and is conserved among eubacteria
    J S McCarty
    Zentrum fur Molekulare Biologie Heidelberg Universitat Heidelberg, FRG
    J Mol Biol 256:829-37. 1996
    ..the sensing of stress and transmission of this information to sigma32, involves the chaperone system formed by the DnaK, DnaJ and GrpE heat shock proteins...
  8. ncbi Structural analysis of substrate binding by the molecular chaperone DnaK
    X Zhu
    Department of Biochemistry and Molecular Biophysics, College of Physicians and Surgeons, Columbia University, New York 10032, USA
    Science 272:1606-14. 1996
    b>DnaK and other members of the 70-kilodalton heat-shock protein (hsp70) family promote protein folding, interaction, and translocation, both constitutively and in response to stress, by binding to unfolded polypeptide segments...
  9. ncbi The Hsp70 and Hsp60 chaperone machines
    B Bukau
    Institut für Biochemie and Molekularbiologie, Universitat Freiburg, Germany
    Cell 92:351-66. 1998
  10. ncbi ClpB cooperates with DnaK, DnaJ, and GrpE in suppressing protein aggregation. A novel multi-chaperone system from Escherichia coli
    M Zolkiewski
    Department of Biochemistry, Kansas State University, Manhattan, Kansas 66506, USA
    J Biol Chem 274:28083-6. 1999
    ..02% of the native activity) because of extensive aggregation. Conventional chaperone systems (GroEL/GroES and DnaK/DnaJ/GrpE) or ClpB alone did not reactivate luciferase under those conditions...
  11. pmc Sequential mechanism of solubilization and refolding of stable protein aggregates by a bichaperone network
    P Goloubinoff
    Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, 91904 Jerusalem, Israel
    Proc Natl Acad Sci U S A 96:13732-7. 1999
    ..We show here that the sequential action of two Escherichia coli chaperone systems, ClpB and DnaK-DnaJ-GrpE, can efficiently solubilize excess amounts of protein aggregates and refold them into active proteins...
  12. ncbi The heat shock response of Escherichia coli
    F Arsene
    Institut fur Biochemie und Molekularbiologie, Freiburg, Germany
    Int J Food Microbiol 55:3-9. 2000
    ..Major HSPs are molecular chaperones, including DnaK, DnaJ and GrpE, and GroEL and GroES, and proteases. They constitute the two major chaperone systems of E...
  13. ncbi Reversible thermal transition in GrpE, the nucleotide exchange factor of the DnaK heat-shock system
    J P Grimshaw
    Biochemisches Institut, Universitat Zurich, CH 8057 Zurich, Switzerland
    J Biol Chem 276:6098-104. 2001
    b>DnaK, a Hsp70 acting in concert with its co-chaperones DnaJ and GrpE, is essential for Escherichia coli to survive environmental stress, including exposure to elevated temperatures...
  14. ncbi The chaperonin GroEL and other heat-shock proteins, besides DnaK, participate in ribosome biogenesis in Escherichia coli
    A El Hage
    Institut de Biologie Physico Chimique, UPR 9073 du CNRS, Paris, France
    Mol Gen Genet 264:796-808. 2001
    It has been shown that in Escherichia coli the chaperone DnaK is necessary for the late stages of 50S and 30S ribosomal subunit assembly in vivo...
  15. ncbi Genetic dissection of the roles of chaperones and proteases in protein folding and degradation in the Escherichia coli cytosol
    T Tomoyasu
    Institut fur Biochemie und Molekularbiologie, Universitat Freiburg, Hermann Herder Str 7, D 79104 Freiburg, Germany
    Mol Microbiol 40:397-413. 2001
    ..folding assistance by chaperones already at 30 degrees C, and showed strong overlap with previously identified DnaK substrates...
  16. ncbi The DnaK chaperone system facilitates 30S ribosomal subunit assembly
    Jennifer A Maki
    Department of Biochemistry, Biophysics, and Molecular Biology, Iowa State University, Ames 50011, USA
    Mol Cell 10:129-38. 2002
    ..Extract activation of in vitro assembly results in association of DnaK/hsp70 chaperone components with pre-30S particles...
  17. ncbi Thermosensor action of GrpE. The DnaK chaperone system at heat shock temperatures
    John P A Grimshaw
    Biochemisches Institut der Universitat Zurich, Winterthurerstrasse 190, CH 8057 Zurich, Switzerland
    J Biol Chem 278:19048-53. 2003
    Temperature directly controls functional properties of the DnaK/DnaJ/GrpE chaperone system...
  18. pmc DnaK-facilitated ribosome assembly in Escherichia coli revisited
    Jean Hervé Alix
    Institut de Biologie Physico Chimique, UPR 9073 du CNRS, F 75005 Paris, France
    RNA 9:787-93. 2003
    Assembly helpers exist for the formation of ribosomal subunits. Such a function has been suggested for the DnaK system of chaperones (DnaK, DnaJ, GrpE)...
  19. pmc rRNA antitermination functions with heat shock promoters
    Hyuk Kyu Seoh
    Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, Massachusetts 02111, USA
    J Bacteriol 185:6486-9. 2003
    ..ability of RNA polymerase elongation complexes that had initiated at three different heat shock promoters, dnaK, groE, and clpB, and then transcribed the antitermination sequence to read through a Rho-dependent terminator...
  20. pmc The solution structure of the bacterial HSP70 chaperone protein domain DnaK(393-507) in complex with the peptide NRLLLTG
    Shawn Y Stevens
    Biophysics Research Division, University of Michigan, Ann Arbor, Michigan 48109 1055, USA
    Protein Sci 12:2588-96. 2003
    ..We present the solution structure of the substrate binding domain (residues 393-507) of the Escherichia coli Hsp70, DnaK, that is bound to the peptide NRLLLTG and compare it to the crystal structure of DnaK(389-607) bound to the same ..
  21. pmc Demonstration of the role of the DnaK chaperone system in assembly of 30S ribosomal subunits using a purified in vitro system
    Jennifer A Maki
    Iowa State University, Department of Biochemistry, Biophysics, and Molecular Biology, Ames, Iowa 50011, USA
    RNA 9:1418-21. 2003
    Recently, there has been controversy regarding the ability of the DnaK chaperone system to facilitate Escherichia coli 30S subunit assembly at otherwise nonpermissive conditions...
  22. ncbi Thermotolerance requires refolding of aggregated proteins by substrate translocation through the central pore of ClpB
    Jimena Weibezahn
    Zentrum fur Molekulare Biologie der Universitat Heidelberg, Universitat Heidelberg, Im Neuenheimer Feld 282, Heidelberg D 69120, Germany
    Cell 119:653-65. 2004
    ..of the ClpB (Hsp104) AAA+ chaperone that solubilizes and reactivates aggregated proteins in concert with the DnaK (Hsp70) chaperone system...
  23. ncbi M domains couple the ClpB threading motor with the DnaK chaperone activity
    Tobias Haslberger
    ZMBH, Universitat Heidelberg, Im Neuenheimer Feld 282, Heidelberg D 69120, Germany
    Mol Cell 25:247-60. 2007
    The AAA(+) chaperone ClpB mediates the reactivation of aggregated proteins in cooperation with the DnaK chaperone system...
  24. pmc Hsp70 chaperone ligands control domain association via an allosteric mechanism mediated by the interdomain linker
    Joanna F Swain
    Department of Biochemistry and Molecular Biology, University of Massachusetts, Amherst, Amherst, MA 01003, USA
    Mol Cell 26:27-39. 2007
    ..We have studied single- and two-domain versions of the E. coli Hsp70, DnaK, to explore the mechanism of interdomain communication...
  25. pmc Collaboration between the ClpB AAA+ remodeling protein and the DnaK chaperone system
    Shannon M Doyle
    Laboratory of Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 104:11138-44. 2007
    ..ClpB and Hsp104 are able to dissolve protein aggregates in conjunction with the DnaK/Hsp70 chaperone system, although the roles of the individual chaperones in disaggregation are not well understood...
  26. ncbi High-throughput screen for small molecules that modulate the ATPase activity of the molecular chaperone DnaK
    Lyra Chang
    Life Sciences Institute, University of Michigan, Ann Arbor, MI 48109, USA
    Anal Biochem 372:167-76. 2008
    b>DnaK is a molecular chaperone of Escherichia coli that belongs to a family of conserved 70-kDa heat shock proteins...
  27. ncbi The Hsp70 chaperone machines of Escherichia coli: a paradigm for the repartition of chaperone functions
    Pierre Genevaux
    Laboratoire de Microbiologie et Genetique Moleculaire, IBCG, CNRS Université Paul Sabatier, 118 route de Narbonne, 31062 Toulouse, Cedex 09, France
    Mol Microbiol 66:840-57. 2007
    ..Genome sequencing has revealed that many organisms contain multiple members of both the DnaK (Hsp70) family and their partner J-domain protein (JDP) cochaperone, belonging to the DnaJ (Hsp40) family...
  28. doi Protein disaggregation by the AAA+ chaperone ClpB involves partial threading of looped polypeptide segments
    Tobias Haslberger
    Zentrum für Molekulare Biologie Heidelberg, DKFZ ZMBH Alliance, Universitat Heidelberg, Im Neuenheimer Feld 282, Heidelberg D 69120, Germany
    Nat Struct Mol Biol 15:641-50. 2008
    The ring-forming AAA+ chaperone ClpB cooperates with the DnaK chaperone system to reactivate aggregated proteins...
  29. doi Molecular basis for regulation of the heat shock transcription factor sigma32 by the DnaK and DnaJ chaperones
    Fernanda Rodriguez
    Zentrum fur Molekulare Biologie der Universitat Heidelberg, DKFZ ZMBH Alliance, Im Neuenheimer Feld 282, 69120 Heidelberg, Germany
    Mol Cell 32:347-58. 2008
    ..is the stress-dependent inhibition of the sigma(32) subunit of RNA polymerase by reversible association with the DnaK chaperone, mediated by the DnaJ cochaperone...
  30. doi Ribosome biogenesis is temperature-dependent and delayed in Escherichia coli lacking the chaperones DnaK or DnaJ
    Abdalla Al Refaii
    CNRS UPR9073 affiliated with University Paris 7 Denis Diderot, Institut de Biologie Physico Chimique, 13 rue Pierre et Marie Curie, Paris, France
    Mol Microbiol 71:748-62. 2009
    In Escherichia coli strains carrying null mutations in either the dnaK or dnaJ genes, the late stages of 30S and 50S ribosomal subunit biogenesis are slowed down in a temperature-dependent manner...
  31. pmc Solution conformation of wild-type E. coli Hsp70 (DnaK) chaperone complexed with ADP and substrate
    Eric B Bertelsen
    Department of Biological Chemistry, University of Michigan, Ann Arbor, MI 48109, USA
    Proc Natl Acad Sci U S A 106:8471-6. 2009
    b>DnaK is the canonical Hsp70 molecular chaperone protein from Escherichia coli...
  32. pmc Mutagenesis reveals the complex relationships between ATPase rate and the chaperone activities of Escherichia coli heat shock protein 70 (Hsp70/DnaK)
    Lyra Chang
    University of Michigan, Ann Arbor, Michigan 48109, USA
    J Biol Chem 285:21282-91. 2010
    The Escherichia coli 70-kDa heat shock protein, DnaK, is a molecular chaperone that engages in a variety of cellular activities, including the folding of proteins...
  33. pmc Species-specific collaboration of heat shock proteins (Hsp) 70 and 100 in thermotolerance and protein disaggregation
    Marika Miot
    Laboratory of Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Proc Natl Acad Sci U S A 108:6915-20. 2011
    ..Hsp104 and ClpB collaborate with the Hsp70 and DnaK chaperone systems, respectively, to retrieve and reactivate stress-denatured proteins from aggregates...
  34. pmc Prokaryotic chaperones support yeast prions and thermotolerance and define disaggregation machinery interactions
    Michael Reidy
    Laboratory of Biochemistry and Genetics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
    Genetics 192:185-93. 2012
    ..Our results define cooperative interactions among these components that are specific or interchangeable across life kingdoms and imply Hsp100 family disaggregases possess intrinsic amyloid remodeling activity...
  35. pmc An interdomain energetic tug-of-war creates the allosterically active state in Hsp70 molecular chaperones
    Anastasia Zhuravleva
    Department of Biochemistry and Molecular Biology, University of Massachusetts, Amherst, MA 01003, USA
    Cell 151:1296-307. 2012
    ..We have trapped this "allosterically active" state for the E. coli Hsp70, DnaK, and identified how interactions among the NBD, the β subdomain of the SBD, the SBD α-helical lid, and the ..
  36. pmc Allosteric opening of the polypeptide-binding site when an Hsp70 binds ATP
    Ruifeng Qi
    Department of Physiology and Biophysics, Virginia Commonwealth University, Richmond, Virginia, USA
    Nat Struct Mol Biol 20:900-7. 2013
    ..These conformational changes, together with our biochemical assays, provide a structural explanation for allosteric coupling in Hsp70 activity. ..
  37. ncbi Authentic precursors to ribosomal subunits accumulate in Escherichia coli in the absence of functional DnaK chaperone
    Aziz El Hage
    Institut de Biologie Physico Chimique, UPR 9073 du CNRS, and University Paris 7 Denis Diderot, 13 rue Pierre et Marie Curie, 75005 Paris, France
    Mol Microbiol 51:189-201. 2004
    Escherichia coli dnaK-ts mutants are defective in the late stages of ribosome biogenesis at high temperature...
  38. ncbi Identification of phosphoproteins in Escherichia coli
    P Freestone
    Department of Microbiology and Immunology, University of Leicester, UK
    Mol Microbiol 15:573-80. 1995
    ..In addition, a calcium-stimulated protein of 70 kDa was identified as the heat-shock protein DnaK, and a 17 kDa lipid-stimulated phosphoprotein as nucleotide diphosphate kinase.
  39. ncbi Involvement of molecular chaperonins in nucleotide excision repair. Dnak leads to increased thermal stability of UvrA, catalytic UvrB loading, enhanced repair, and increased UV resistance
    Y Zou
    Sealy Center for Molecular Science, University of Texas Medical Branch, Galveston, Texas 77555 1071, USA
    J Biol Chem 273:12887-92. 1998
    ..coli molecular chaperones DnaK or the combination of DnaK, DnaJ, and GrpE...
  40. ncbi Interaction of the DnaK and DnaJ chaperone system with a native substrate, P1 RepA
    Soon Young Kim
    Laboratory of Molecular Biology, NCI, National Institutes of Health, Bethesda, Maryland 20892 4264, USA
    J Biol Chem 277:44778-83. 2002
    b>DnaK, the Hsp70 chaperone of Escherichia coli interacts with protein substrates in an ATP-dependent manner, in conjunction with DnaJ and GrpE co-chaperones, to carry out protein folding, protein remodeling, and assembly and disassembly of ..
  41. ncbi Evaluation of similarities in the cis/trans isomerase function of trigger factor and DnaK
    Cordelia Schiene-Fischer
    Max Planck Research Unit for Enzymology of Protein Folding, Weinbergweg 22, D 06120 Halle Saale, Germany
    Biol Chem 383:1865-73. 2002
    Two functionally redundant enzymes, trigger factor and the hsp70 chaperone DnaK, have been found to assist de novo protein folding in E coli...
  42. doi Monitoring protein conformation along the pathway of chaperonin-assisted folding
    Shruti Sharma
    Department of Cellular Biochemistry, Max Planck Institute of Biochemistry, Am Klopferspitz 18, D 82152 Martinsried, Germany
    Cell 133:142-53. 2008
    ..Newly synthesized proteins reach GroEL via transfer from upstream chaperones such as DnaK/DnaJ (Hsp70)...
  43. ncbi Function of DnaJ and DnaK as chaperones in origin-specific DNA binding by RepA
    S Wickner
    Laboratory of Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892
    Nature 350:165-7. 1991
    ..The Escherichia coli hsp70 analogue, DnaK, and two other heat-shock proteins, DnaJ and GrpE, are required for cell viability at high temperatures and are ..
  44. ncbi DnaK, DnaJ, and GrpE heat shock proteins negatively regulate heat shock gene expression by controlling the synthesis and stability of sigma 32
    D Straus
    Department of Bacteriology, University of Wisconsin, Madison 53706
    Genes Dev 4:2202-9. 1990
    The Escherichia coli DnaK heat shock protein has been identified previously as a negative regulator of E. coli heat shock gene expression...
  45. pmc Isolation and characterization of Escherichia coli mutants that lack the heat shock sigma factor sigma 32
    Y N Zhou
    Department of Bacteriology, University of Wisconsin, Madison 53706
    J Bacteriol 170:3640-9. 1988
    ..b>DnaK protein synthesis cannot be detected at low temperature, but can be detected after a shift to 42 degrees C...
  46. ncbi ClpB and HtpG facilitate de novo protein folding in stressed Escherichia coli cells
    J G Thomas
    Department of Chemical Engineering, University of Washington, Seattle 98195, USA
    Mol Microbiol 36:1360-70. 2000
    b>DnaK-DnaJ-GrpE and GroEL-GroES are the best-characterized molecular chaperone systems in the cytoplasm of Escherichia coli...
  47. ncbi Thermodynamics of heat-shock response
    K Bourke Arnvig
    Institute of Molecular Biology, Oster Farimagsgade 2A, 1353 Copenhagen K, Denmark
    Phys Rev Lett 84:3005-8. 2000
    ..The heat-shock response of protein DnaK synthesis in E...
  48. ncbi Microbial molecular chaperones
    P A Lund
    School of Biosciences, University of Birmingham, Birmingham B15 2TT, UK
    Adv Microb Physiol 44:93-140. 2001
    ..The two major chaperone systems in bacterial cells (as typified by Escherichia coli) are the GroE and DnaK chaperones, and the contrasting roles and mechanisms of these chaperones will be presented...
  49. pmc Effects of disruption of heat shock genes on susceptibility of Escherichia coli to fluoroquinolones
    Yuko Yamaguchi
    Department of Microbiology and Molecular Genetics, Graduate School of Pharmaceutical Sciences, Chiba University, Japan
    BMC Microbiol 3:16. 2003
    ..Analogously, the proteins induced in bacterial cells exposed to antibiotics are believed to affect the organisms' susceptibility to these agents...
  50. ncbi The DnaK-DnaJ-GrpE chaperone system activates inert wild type pi initiator protein of R6K into a form active in replication initiation
    Shamsu Zzaman
    Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston, South Carolina 29425, USA
    J Biol Chem 279:50886-94. 2004
    ..Here we show that the chaperone DnaK along with its co-chaperone DnaJ and the nucleotide exchange factor GrpE were needed to activate WT pi and caused ..
  51. ncbi Interactions within the ClpB/DnaK bi-chaperone system from Escherichia coli
    Sabina Kedzierska
    Department of Biochemistry, University of Gdansk, Gdansk, Poland
    Arch Biochem Biophys 444:61-5. 2005
    ClpB and DnaK form a bi-chaperone system that reactivates strongly aggregated proteins in vivo and in vitro...
  52. ncbi An essential role for the DnaK molecular chaperone in stabilizing over-expressed substrate proteins of the bacterial twin-arginine translocation pathway
    Ritsdeliz Perez-Rodriguez
    School of Chemical and Biomolecular Engineering, Cornell University, Ithaca, NY 14853, USA
    J Mol Biol 367:715-30. 2007
    ..case of the Sec pathway, this function is carried out by the dedicated SecB chaperone and the general chaperones DnaK-DnaJ-GrpE and GroEL-GroES, whose job collectively is to render substrate proteins partially or entirely unfolded ..
  53. doi Construction of Escherichia coli dnaK-deletion mutant infected by lambdaDE3 for overexpression and purification of recombinant GrpE proteins
    Shinya Sugimoto
    Laboratory of Microbial Technology, Division of Microbial Science and Technology, Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School, Kyushu University, 6 10 1 Hakozaki, Higashi ku, Fukuoka 812 8581, Japan
    Protein Expr Purif 60:31-6. 2008
    ..However, contamination of molecular chaperone DnaK during purification of the recombinant protein is sometimes a problem, since DnaK sometimes has a negative effect ..
  54. doi Cyclophilin PpiB is involved in motility and biofilm formation via its functional association with certain proteins
    Aggeliki Skagia
    Laboratory of General and Agricultural Microbiology, Faculty of Crop Science, Agricultural University of Athens, Iera Odos 75, 11855, Athens, Greece
    Genes Cells 21:833-51. 2016
    ..For DnaK, acetyl-CoA carboxylase, biotin carboxylase subunit (AccC) and phosphate acetyltransferase (Pta) we also showed a ..
  55. pmc Suppression of a dnaKJ deletion by multicopy dksA results from non-feedback-regulated transcripts that originate upstream of the major dksA promoter
    Pete Chandrangsu
    Department of Bacteriology, University of Wisconsin Madison, Madison, Wisconsin, USA
    J Bacteriol 194:1437-46. 2012
    ....
  56. ncbi The Escherichia coli DnaK chaperone, the 70-kDa heat shock protein eukaryotic equivalent, changes conformation upon ATP hydrolysis, thus triggering its dissociation from a bound target protein
    K Liberek
    Department of Molecular Biology, University of Gdansk, Poland
    J Biol Chem 266:14491-6. 1991
    The DnaK protein of Escherichia coli and its eukaryotic hsp70 analogues are known to bind some polypeptides and to release or dissociate from them following ATP hydrolysis...
  57. pmc AppppA binds to several proteins in Escherichia coli, including the heat shock and oxidative stress proteins DnaK, GroEL, E89, C45 and C40
    D B Johnstone
    Department of Cellular and Molecular Toxicology, Harvard School of Public Health, Boston, MA 02115
    EMBO J 10:3897-904. 1991
    ..We report here that several E. coli proteins bind AppppA, including the heat shock and oxidative stress proteins DnaK, GroEL, E89, C45 and C40...
  58. ncbi Physiological roles of the DnaK and GroE stress proteins: catalysts of protein folding or macromolecular sponges?
    R A LaRossa
    Agricultural Products Department, Stine Haskell Research Center S300 333, E I Du Pont de Nemours and Company, Newark, Delaware 19714
    Mol Microbiol 5:529-34. 1991
    ..response is characterized by the induction of several proteins, three of which are the 70 kilodalton product of the dnaK gene, the 60 kilodalton product of the groEL (mopA) gene and the 15 kilodalton product of the groES (mopB) gene...
  59. ncbi Transient shut off of Escherichia coli heat shock protein synthesis upon temperature shift down
    T Taura
    Institute for Virus Research, Kyoto University, Japan
    Biochem Biophys Res Commun 163:438-43. 1989
    ..to 24 degrees C in strain K-12) was found to depress markedly the synthesis of major heat shock proteins GroEL and DnaK in E. coli. The depression was transient and cancelled gradually to a new steady state level, taking 60-80 min...
  60. pmc Escherichia coli dnaK null mutants are inviable at high temperature
    K H Paek
    J Bacteriol 169:283-90. 1987
    b>DnaK, a major Escherichia coli heat shock protein, is homologous to major heat shock proteins (Hsp70s) of Drosophila melanogaster and humans...
  61. pmc Major heat shock gene of Drosophila and the Escherichia coli heat-inducible dnaK gene are homologous
    J C Bardwell
    Proc Natl Acad Sci U S A 81:848-52. 1984
    The Escherichia coli dnaK gene is homologous to the major heat shock-induced gene in Drosophila (Hsp70)...
  62. pmc Both ambient temperature and the DnaK chaperone machine modulate the heat shock response in Escherichia coli by regulating the switch between sigma 70 and sigma 32 factors assembled with RNA polymerase
    A Blaszczak
    Department of Molecular Biology, University of Gdansk, Poland
    EMBO J 14:5085-93. 1995
    ..At least three heat shock proteins, DnaK, DnaJ and GrpE, are involved in negative modulation of the sigma 32-dependent heat shock response...
  63. pmc The DnaJ chaperone catalytically activates the DnaK chaperone to preferentially bind the sigma 32 heat shock transcriptional regulator
    K Liberek
    Department of Molecular Biology, University of Gdansk, Poland
    Proc Natl Acad Sci U S A 92:6224-8. 1995
    ..Three of the heat shock proteins, DnaK, DnaI, and GrpE, play a central role in the negative autoregulation of this response at the transcriptional level...
  64. ncbi Protein folding in the cytoplasm of Escherichia coli: requirements for the DnaK-DnaJ-GrpE and GroEL-GroES molecular chaperone machines
    J G Thomas
    Department of Chemical Engineering, University of Washington, Seattle 98195 1750, USA
    Mol Microbiol 21:1185-96. 1996
    ..systematically investigated the influence of mutations in the sigma(32) heat-shock transcription factor and the DnaK-DnaJ-GrpE and GroEL-GroES molecular chaperone machines on the folding of preS2-beta-galactosidase...
  65. ncbi NMR solution structure of the 21 kDa chaperone protein DnaK substrate binding domain: a preview of chaperone-protein interaction
    H Wang
    Biophysics Research Division, Department of Biological Chemistry, The University of Michigan, Ann Arbor 48109, USA
    Biochemistry 37:7929-40. 1998
    The solution structure of the 21 kDa substrate-binding domain of the Escherichia coli Hsp70-chaperone protein DnaK (DnaK 386-561) has been determined to a precision of 1...
  66. ncbi Levels of DnaK and DnaJ provide tight control of heat shock gene expression and protein repair in Escherichia coli
    T Tomoyasu
    Institut fur Biochemie und Molekularbiologie, Universitat Freiburg, Hermann Herder Str 7, D 79104 Freiburg, Germany
    Mol Microbiol 30:567-81. 1998
    ..Modulators are the DnaK chaperone system, which inactivates and destabilizes sigma32, and the FtsH protease, which is largely responsible ..
  67. ncbi Heat shock regulation in the ftsH null mutant of Escherichia coli: dissection of stability and activity control mechanisms of sigma32 in vivo
    T Tatsuta
    Department of Molecular Cell Biology, Institute of Molecular Embryology and Genetics, Kumamoto University School of Medicine, Kumamoto 862 0976, Japan
    Mol Microbiol 30:583-93. 1998
    ..Overproduction of DnaK/J reduces the activity of sigma32 in DeltaftsH cells without any detectable changes in the level of sigma32, ..
  68. ncbi On the mechanism of FtsH-dependent degradation of the sigma 32 transcriptional regulator of Escherichia coli and the role of the Dnak chaperone machine
    A Blaszczak
    Polish Academy of Sciences, University of Gdansk, Kladki, Poland
    Mol Microbiol 31:157-66. 1999
    ..Purified sigma 32 C delta interacted with the DnaK and DnaJ chaperone proteins in a fashion similar to that of wild-type sigma 32...
  69. pmc Elongation factor Tu and DnaK are transferred from the cytoplasm to the periplasm of Escherichia coli during osmotic downshock presumably via the mechanosensitive channel mscL
    C Berrier
    Laboratoire des Biomembranes, UMR CNRS 8619, Universite Paris Sud, 91405 Orsay Cedex, France
    J Bacteriol 182:248-51. 2000
    Upon osmotic downshock, a few cytoplasmic proteins, including thioredoxin, elongation factor Tu (EF-Tu), and DnaK, are released from Tris-EDTA-treated Escherichia coli cells by an unknown mechanism...
  70. pmc DnaK dependence of mutant ethanol oxidoreductases evolved for aerobic function and protective role of the chaperone against protein oxidative damage in Escherichia coli
    Pedro Echave
    Departamento de Biologia Molecular y Biotecnologia, Instituto de Investigaciones Biomedicas, Universidad Nacional Autonoma de Mexico, P O Box 70 228, 04510 Mexico City, Mexico
    Proc Natl Acad Sci U S A 99:4626-31. 2002
    ..ability on ethanol depends also on protection of the mutant AdhE against metal-catalyzed oxidation by the chaperone DnaK (a member of the Hsp70 family). No DnaK protection of the enzyme is evident during anaerobic growth on glucose...
  71. ncbi The molecular chaperone DnaK is not recruited to translating ribosomes that lack trigger factor
    Gisela Kramer
    Department of Chemistry and Biochemistry, University of Texas, Austin, TX 78712, USA
    Arch Biochem Biophys 403:63-70. 2002
    The molecular chaperone DnaK and trigger factor (TF), a ribosome-associated protein with folding activity, have been implicated in assisting nascent polypeptides to acquire a three-dimensional structure on Escherichia coli ribosomes...
  72. ncbi Prevention and reversion of protein aggregation by molecular chaperones in the E. coli cytosol: implications for their applicability in biotechnology
    Christian Schlieker
    Institut fur Biochemie und Molekularbiologie, Hermann Herder Str 7, 79104, Freiburg, Germany
    J Biotechnol 96:13-21. 2002
    ..Such resolubilization of aggregated proteins is mediated by a bi-chaperone system consisting of ClpB and DnaK, the prokaryotic representatives of the Hsp100 and Hsp70 families...
  73. ncbi Effect of mutations in dnaK and dnaJ genes on cysteine operon expression in Escherichia coli
    P Karpinski
    Department of Bacterial Genetics, Institute of Microbiology, Warsaw University, 02 096 Warsaw, Poland
    Folia Microbiol (Praha) 47:371-4. 2002
    The effect of mutations in dnaK and dnaJ genes on the expression of two operons that are part of cysteine regulon was determined using Escherichia coli strains harboring cysPTWA::lacZ and cysJIH::lacZ fusions...
  74. ncbi Protein folding and degradation in bacteria: to degrade or not to degrade? That is the question
    D A Dougan
    Institut fur Biochemie und Molekularbiologie, Universitat Freiburg, Hermann Herder Str 7, Freiburg 79104, Germany
    Cell Mol Life Sci 59:1607-16. 2002
    ..Central to this network are two protein families, the AAA+ and the Hsp70 family. The major Hsp70 chaperone. DnaK, efficiently prevents protein aggregation and supports the refolding of damaged proteins...
  75. ncbi The J-domain of Hsp40 couples ATP hydrolysis to substrate capture in Hsp70
    Pernilla Wittung-Stafshede
    Department of Chemistry, Tulane University, New Orleans, Louisiana 70118, USA
    Biochemistry 42:4937-44. 2003
    The Escherichia coli Hsp40 DnaJ uses its J-domain to target substrate polypeptides for binding to the Hsp70 DnaK, but the mechanism of J-domain function has been obscured by a substrate-like interaction between DnaJ and DnaK...
  76. pmc Functional dissection of Escherichia coli trigger factor: unraveling the function of individual domains
    G Kramer
    Zentrum für Molekulare Biologie ZMBH, Universitat Heidelberg, Im Neuenheimer Feld 282, D 69120 Heidelberg, Germany
    J Bacteriol 186:3777-84. 2004
    ..the N domain (N, NP, NC) complemented the synthetic lethality of Deltatig DeltadnaK in cells lacking TF and DnaK, prevented protein aggregation in these cells, and cross-linked to nascent polypeptides in vitro...
  77. ncbi Effects of high static magnetic field exposure on different DNAs
    Lucia Potenza
    Istituto di Chimica Biologica Giorgio Fornaini, Universita degli Studi di Urbino, Urbino PU, Italy
    Bioelectromagnetics 25:352-5. 2004
    ..This genotoxic effect of the magnetic field, however, is minimized in living organisms due to the presence of protective cellular responses...
  78. ncbi Bacterial heat shock proteins enhance class II MHC antigen processing and presentation of chaperoned peptides to CD4+ T cells
    Aaron A R Tobian
    Department of Pathology, Case Western Reserve University, Cleveland, OH 44106, USA
    J Immunol 173:5130-7. 2004
    ..Our studies revealed that exogenous bacterial HSPs (Escherichia coli DnaK and Mycobacterium tuberculosis HSP70) delivered an extended OVA peptide for processing and MHC-II presentation, as ..
  79. ncbi Effect of the polypeptide binding on the thermodynamic stability of the substrate binding domain of the DnaK chaperone
    Naoki Tanaka
    Department of Polymer Science and Engineering, Kyoto Institute of Technology, Matsugasaki, Sakyo, Kyoto 606 8585, Japan
    Biochim Biophys Acta 1748:1-8. 2005
    The effect of polypeptide binding on the stability of the substrate binding domain of the molecular chaperone DnaK has been studied by thermodynamic analysis...
  80. ncbi Folding of a misfolding-prone beta-galactosidase in absence of DnaK
    Elena García-Fruitós
    Institut de Biotecnologia i de Biomedicina and Departament de Genètica i de Microbiologia, Universitat Autonoma de Barcelona, Bellaterra, 08193 Barcelona, Spain
    Biotechnol Bioeng 90:869-75. 2005
    In absence of chaperone DnaK, bacterially produced misfolding-prone proteins aggregate into large inclusion bodies, but still a significant part of these polypeptides remains in the soluble cell fraction...
  81. ncbi The Hsp70 Ssz1 modulates the function of the ribosome-associated J-protein Zuo1
    Peggy Huang
    Department of Biochemistry, University of Wisconsin, Madison, Wisconsin 53706, USA
    Nat Struct Mol Biol 12:497-504. 2005
    ....
  82. pmc Defense against protein carbonylation by DnaK/DnaJ and proteases of the heat shock regulon
    Asa Fredriksson
    Department of Cell and Molecular Biology, Microbiology, Medicinaregatan 9C, 413 90 Goteborg, Sweden
    J Bacteriol 187:4207-13. 2005
    ..Separate overproduction of the major chaperone systems, DnaK/DnaJ and GroEL/GroES, established that the former of these is more important in counteracting protein carbonylation...
  83. pmc Not all J domains are created equal: implications for the specificity of Hsp40-Hsp70 interactions
    Fritha Hennessy
    Department of Biochemistry, Microbiology and Biotechnology, Rhodes University, Grahamstown, South Africa
    Protein Sci 14:1697-709. 2005
    ..We also propose a model in which the J domain is able to integrate specificity and chaperone activity...
  84. ncbi Direct comparison of a stable isolated Hsp70 substrate-binding domain in the empty and substrate-bound states
    Joanna F Swain
    Department of Biochemistry and Molecular Biology, University of Massachusetts, Amherst, Massachusetts 01003, USA
    J Biol Chem 281:1605-11. 2006
    ..We have generated a new stable construct of the substrate-binding domain from the Escherichia coli Hsp70, DnaK, which has enabled us to compare the empty and peptide-bound conformations using NMR chemical shift analysis and ..
  85. ncbi Synthesis of DnaK and GroEL in Escherichia coli cells exposed to different magnetic field signals
    Brunella Del Re
    Department of Evolutionary Experimental Biology, University of Bologna, Via Selmi 3, 40126 Bologna, Italy
    Bioelectrochemistry 69:99-103. 2006
    ..It was found that bacteria exposed to SMF showed a significantly higher level of DnaK and GroEL proteins as compared to sham-exposed bacteria as revealed by Western blot, whereas a lower level was ..
  86. ncbi DNA repeat rearrangements mediated by DnaK-dependent replication fork repair
    Stephen J Goldfless
    Department of Biology and Rosenstiel Basic Medical Sciences Research Center, Brandeis University, Waltham, Massachusetts 02454, USA
    Mol Cell 21:595-604. 2006
    ..We provide evidence here that the DnaK chaperone of E. coli controls this template switch repair process...
  87. ncbi Indole-3-acetic acid improves Escherichia coli's defences to stress
    C Bianco
    Institute of Genetics and Biophysics, Adriano Buzzati Traverso, Via P Castellino 111, 80131 Naples, Italy
    Arch Microbiol 185:373-82. 2006
    ..toxic compounds (antibiotics, detergents and dyes) and this correlates with higher levels of the heat shock protein DnaK. We suggest that IAA triggers an increased level of alert and protection against external adverse conditions by ..
  88. ncbi Fatty acyl benzamido antibacterials based on inhibition of DnaK-catalyzed protein folding
    Markus Liebscher
    Max Planck Research Unit for Enzymology of Protein Folding, Weinbergweg 22, 06120 Halle Saale, Germany
    J Biol Chem 282:4437-46. 2007
    We have reported that the hsp70 chaperone DnaK from Escherichia coli might assist protein folding by catalyzing the cis/trans isomerization of secondary amide peptide bonds in unfolded or partially folded proteins...
  89. ncbi DnaK plays a pivotal role in Tat targeting of CueO and functions beside SlyD as a general Tat signal binding chaperone
    Wenke Graubner
    Institute of Microbiology, University of Halle Wittenberg, Kurt Mothes Strasse 3, D 06120 Halle, Germany
    J Biol Chem 282:7116-24. 2007
    ..Here we show that the chaperones SlyD and DnaK bind to a broad range of different Tat signal sequences in vitro and in vivo...
  90. pmc In vivo modulation of a DnaJ homolog, CbpA, by CbpM
    Matthew R Chenoweth
    Laboratory of Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    J Bacteriol 189:3635-8. 2007
    CbpA, an Escherichia coli DnaJ homolog, can function as a cochaperone for the DnaK/Hsp70 chaperone system, and its in vitro activity can be modulated by CbpM...
  91. pmc SigmaE regulates and is regulated by a small RNA in Escherichia coli
    Karl M Thompson
    Laboratory of Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    J Bacteriol 189:4243-56. 2007
    ..An insertion in the rep helicase and one upstream of dnaK decreased expression of the fusion...
  92. ncbi Design of transcriptional fusions of stress sensitive promoters and GFP to monitor the overburden of Escherichia coli hosts during recombinant protein production
    Sabine Nemecek
    Department of Biotechnology, University of Natural Resources and Applied Life Sciences, Vienna, Austria
    Bioprocess Biosyst Eng 31:47-53. 2008
    ..Results of efficient stress monitoring approaches in ongoing protein production process are presented...
  93. ncbi Divergent genetic control of protein solubility and conformational quality in Escherichia coli
    Elena García-Fruitós
    Institute for Biotechnology and Biomedicine, Department of Genetics and Microbiology and CIBER BBN Networking Centre on Bioengineering, Biomaterials and Nanomedicine, Autonomous University of Barcelona, Bellaterra, 08193 Barcelona, Spain
    J Mol Biol 374:195-205. 2007
    ..Most of the tested genetic deficiencies in different cytosolic chaperones and proteases (affecting DnaK, GroEL, GroES, ClpB, ClpP and Lon at different extents) resulted in much less soluble but unexpectedly more ..
  94. doi Photophysical properties of popular fluorescent adenosine nucleotide analogs used in enzyme mechanism probing
    Adriane Leskovar
    Max Planck Institute for Medical Research, Department of Biomolecular Mechanisms, Jahnstrasse 29, D 69120 Heidelberg, Germany
    Arch Biochem Biophys 473:16-24. 2008
    ..The measured quantum yields quantify the increase in fluorescence for (C8)-MABA-ADP, MANT-ATP and (Pgamma)-MABA-ATP as 153%, 93% and 14% when bound to DnaK, ClpB and Trap1, respectively, compared to free in buffer solution.
  95. pmc Role of global regulators and nucleotide metabolism in antibiotic tolerance in Escherichia coli
    Sonja Hansen
    Antimicrobial Discovery Center and Department of Biology, Northeastern University, Boston, MA 02115, USA
    Antimicrob Agents Chemother 52:2718-26. 2008
    ..The genes affected in these strains were dnaJ and dnaK (chaperones), apaH (diadenosine tetraphosphatase), surA (peptidyl-prolyl cis-trans isomerase), fis and hns (global ..
  96. pmc DnaK, DnaJ, and GrpE are required for flagellum synthesis in Escherichia coli
    W Shi
    Department of Biochemistry, University of Wisconsin, Madison 53706
    J Bacteriol 174:6256-63. 1992
    The DnaK, DnaJ, and GrpE heat shock proteins are required for motility of Escherichia coli...
  97. pmc The dnaK gene of Escherichia coli functions in initiation of chromosome replication
    Y Sakakibara
    Department of Chemistry, National Institute of Health, Tokyo, Japan
    J Bacteriol 170:972-9. 1988
    A newly isolated dnaK mutant of Escherichia coli, which contains the mutation dnaK111, has been found to be conditionally defective in initiation of DNA replication...
  98. ncbi The nucleotide sequence of the Escherichia coli K12 dnaJ+ gene. A gene that encodes a heat shock protein
    J C Bardwell
    J Biol Chem 261:1782-5. 1986
    ..We present evidence that the rate of expression of the dnaJ protein is increased by heat shock under the control of the htpR (rpoH) gene product...
  99. ncbi The NH2-terminal 108 amino acids of the Escherichia coli DnaJ protein stimulate the ATPase activity of DnaK and are sufficient for lambda replication
    D Wall
    Department of Cellular, Viral, and Molecular Biology, University of Utah Medical Center, Salt Lake City 84132
    J Biol Chem 269:5446-51. 1994
    The Escherichia coli heat shock proteins DnaK and DnaJ function cooperatively as molecular chaperones. Central to their biochemical functions is the ability of DnaJ to interact with DnaK and to stimulate its ATPase activity...
  100. pmc DnaK mutants defective in ATPase activity are defective in negative regulation of the heat shock response: expression of mutant DnaK proteins results in filamentation
    J S McCarty
    Department of Biology, Massachusetts Institute of Technology, Cambridge 02139
    J Bacteriol 176:764-80. 1994
    Site-directed mutagenesis has previously been used to construct Escherichia coli dnaK mutants encoding proteins that are altered at the site of in vitro phosphorylation (J. S. McCarty and G. C. Walker, Proc. Natl. Acad. Sci...
  101. ncbi The cadmium-stress stimulon of Escherichia coli K-12
    P Ferianc
    Institute of Microbiology, Slovak Academy of Sciences, Bratislava, Slovak Republic
    Microbiology 144:1045-50. 1998
    ..The role of the CDPs is discussed in view of their physiological assignments in the cell...