dnaG

Summary

Gene Symbol: dnaG
Description: DNA primase
Alias: ECK3056, JW3038, dnaP, parB, sdgA
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. ncbi Identification of a domain of Escherichia coli primase required for functional interaction with the DnaB helicase at the replication fork
    K Tougu
    Molecular Biology Program, Memorial Sloan Kettering Cancer Center, New York, New York 10021
    J Biol Chem 269:4675-82. 1994
  2. ncbi Monomeric solution structure of the helicase-binding domain of Escherichia coli DnaG primase
    Xun Cheng Su
    Research School of Chemistry, Australian National University, Canberra, ACT 0200, Australia
    FEBS J 273:4997-5009. 2006
  3. ncbi Crystal and solution structures of the helicase-binding domain of Escherichia coli primase
    Aaron J Oakley
    Research School of Chemistry, Australian National University, Canberra, Australian Capital Territory 0200, Australia
    J Biol Chem 280:11495-504. 2005
  4. ncbi Expression, purification, crystallization, and NMR studies of the helicase interaction domain of Escherichia coli DnaG primase
    Karin Loscha
    Research School of Chemistry, Australian National University, Canberra, ACT 0200, Australia
    Protein Expr Purif 33:304-10. 2004
  5. ncbi The interaction between helicase and primase sets the replication fork clock
    K Tougu
    Graduate Program in Molecular Biology, Cornell University Graduate School of Medical Sciences, New York, New York 10021, USA
    J Biol Chem 271:21398-405. 1996
  6. ncbi Trading places on DNA--a three-point switch underlies primer handoff from primase to the replicative DNA polymerase
    A Yuzhakov
    The Rockefeller University, New York, New York 10021, USA
    Cell 96:153-63. 1999
  7. ncbi Structure of the RNA polymerase domain of E. coli primase
    J L Keck
    Department of Molecular and Cell Biology, University of California, Berkeley, 229 Stanley Hall, No 3206, Berkeley, CA 94720, USA
    Science 287:2482-6. 2000
  8. ncbi Identification of a region of Escherichia coli DnaB required for functional interaction with DnaG at the replication fork
    P Chang
    Molecular Biology Graduate Program, Weill Graduate School of Medical Sciences of Cornell University and the Molecular Biology Program, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    J Biol Chem 275:26187-95. 2000
  9. ncbi Cloning of bacterial DNA replication genes in bacteriophage lambda
    L Rowen
    Mol Gen Genet 187:501-9. 1982
  10. ncbi The ordered assembly of the phiX174-type primosome. I. Isolation and identification of intermediate protein-DNA complexes
    J Y Ng
    Graduate Program in Molecular Biology, Cornell University Graduate School of Medical Sciences, New York, New York 10021, USA
    J Biol Chem 271:15642-8. 1996

Scientific Experts

  • G Duval-Valentin
  • M A Schumacher
  • P Soultanas
  • G N Godson
  • Mark A Griep
  • Patrick M Schaeffer
  • Nicholas E Dixon
  • Jacob E Corn
  • K J Marians
  • Divya Nandakumar
  • Karin V Loscha
  • Natalie Naue
  • Gottfried Otting
  • Aaron J Oakley
  • Tranum Kaur
  • Monika Maciag
  • Elsje Pienaar
  • Scott A Koepsell
  • Nathan A Tanner
  • Steven H Hinrichs
  • Marilynn A Larson
  • Atul Agarwal
  • Simon Ringgaard
  • Kiran Chintakayala
  • James M Berger
  • Anna Rodina
  • Xun Cheng Su
  • W Sun
  • Kenn Gerdes
  • Yanina R Sevastsyanovich
  • S Bhattacharyya
  • H Hiasa
  • Matthew C J Wilce
  • Gitte Ebersbach
  • Karin Loscha
  • Atanaska V Mitkova
  • Manjula Pandey
  • Smita S Patel
  • J Y Ng
  • Yi Zhang
  • Andrea Bogutzki
  • Monika Beerbaum
  • Ute Curth
  • Peter Schmieder
  • J R Lupski
  • Barbara E Funnell
  • Qusai Al Abdallah
  • Roderick A Slavcev
  • Nafiseh Nafissi
  • Shawn Wettig
  • S K Johnson
  • M Podobnik
  • K Tougu
  • J L Keck
  • P Chang
  • Agnieszka Szalewska-Pałasz
  • Maja Kochanowska
  • Robert Lyzeń
  • Grzegorz Wegrzyn
  • M O'DONNELL
  • Samir M Hamdan
  • L Powers
  • A Yuzhakov
  • Slobodan Jergic
  • Jeffrey G Pelton
  • Hendrik J Viljoen
  • Antoine M van Oijen
  • Christopher A Frey
  • David M Nelson
  • Jan Lowe
  • Steven D Podos
  • Michael J Pucci
  • Jijun Cheng
  • William H Grainger
  • Cuixian Liu
  • Shirley Louise-May
  • Avinash S Phadke
  • Barton J Bradbury
  • Jane A Thanassi
  • Jason A Wiles
  • Christy Thoma
  • Milind S Deshpande
  • David J Scott
  • J Versalovic
  • T Asai
  • Pamela H P Gan
  • J R Swart
  • Paul J Pease
  • Christopher M Thomas
  • Guido Pintacuda

Detail Information

Publications91

  1. ncbi Identification of a domain of Escherichia coli primase required for functional interaction with the DnaB helicase at the replication fork
    K Tougu
    Molecular Biology Program, Memorial Sloan Kettering Cancer Center, New York, New York 10021
    J Biol Chem 269:4675-82. 1994
    ....
  2. ncbi Monomeric solution structure of the helicase-binding domain of Escherichia coli DnaG primase
    Xun Cheng Su
    Research School of Chemistry, Australian National University, Canberra, ACT 0200, Australia
    FEBS J 273:4997-5009. 2006
    b>DnaG is the primase that lays down RNA primers on single-stranded DNA during bacterial DNA replication...
  3. ncbi Crystal and solution structures of the helicase-binding domain of Escherichia coli primase
    Aaron J Oakley
    Research School of Chemistry, Australian National University, Canberra, Australian Capital Territory 0200, Australia
    J Biol Chem 280:11495-504. 2005
    During bacterial DNA replication, the DnaG primase interacts with the hexameric DnaB helicase to synthesize RNA primers for extension by DNA polymerase...
  4. ncbi Expression, purification, crystallization, and NMR studies of the helicase interaction domain of Escherichia coli DnaG primase
    Karin Loscha
    Research School of Chemistry, Australian National University, Canberra, ACT 0200, Australia
    Protein Expr Purif 33:304-10. 2004
    In Escherichia coli, the DnaG primase is the RNA polymerase that synthesizes RNA primers at replication forks...
  5. ncbi The interaction between helicase and primase sets the replication fork clock
    K Tougu
    Graduate Program in Molecular Biology, Cornell University Graduate School of Medical Sciences, New York, New York 10021, USA
    J Biol Chem 271:21398-405. 1996
    ..Thus, the interaction between DnaG and DnaB at the replication fork is the primary regulator of the cycle of Okazaki fragment synthesis.
  6. ncbi Trading places on DNA--a three-point switch underlies primer handoff from primase to the replicative DNA polymerase
    A Yuzhakov
    The Rockefeller University, New York, New York 10021, USA
    Cell 96:153-63. 1999
    ..The conservation of this three-point switch, in which two proteins exchange places on DNA via mutually exclusive interaction with a third protein, is discussed...
  7. ncbi Structure of the RNA polymerase domain of E. coli primase
    J L Keck
    Department of Molecular and Cell Biology, University of California, Berkeley, 229 Stanley Hall, No 3206, Berkeley, CA 94720, USA
    Science 287:2482-6. 2000
    ..The high-resolution crystal structure of a primase, comprising the catalytic core of the Escherichia coli DnaG protein, was determined...
  8. ncbi Identification of a region of Escherichia coli DnaB required for functional interaction with DnaG at the replication fork
    P Chang
    Molecular Biology Graduate Program, Weill Graduate School of Medical Sciences of Cornell University and the Molecular Biology Program, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    J Biol Chem 275:26187-95. 2000
    ..of the replicative primosomes of Escherichia coli are provided by DnaB, the replication fork DNA helicase, and DnaG, the Okazaki fragment primase...
  9. ncbi Cloning of bacterial DNA replication genes in bacteriophage lambda
    L Rowen
    Mol Gen Genet 187:501-9. 1982
    ..lambda phages containing the genes for dnaZ protein (the gamma subunit of DNA polymerse III holoenzyme), primase (dnaG protein) and dnaC protein from Escherichia coli and Salmonella typhimurium were isolated. Each gene cloned from S...
  10. ncbi The ordered assembly of the phiX174-type primosome. I. Isolation and identification of intermediate protein-DNA complexes
    J Y Ng
    Graduate Program in Molecular Biology, Cornell University Graduate School of Medical Sciences, New York, New York 10021, USA
    J Biol Chem 271:15642-8. 1996
    ..A transient interaction between the preprimosome and DnaG generates the five-protein primosome. As described in an accompanying article (Ng, J. Y., and Marians, K. J...
  11. pmc Direct physical interaction between DnaG primase and DnaB helicase of Escherichia coli is necessary for optimal synthesis of primer RNA
    Y B Lu
    Department of Microbiology, Duke University Medical Center, Durham, NC 27710, USA
    Proc Natl Acad Sci U S A 93:12902-7. 1996
    The primase DnaG of Escherichia coli requires the participation of the replicative helicase DnaB for optimal synthesis of primer RNA for lagging strand replication...
  12. ncbi A TOPRIM domain in the crystal structure of the catalytic core of Escherichia coli primase confirms a structural link to DNA topoisomerases
    M Podobnik
    Laboratories of Molecular Biophysics, Howard Hughes Medical Institute, The Rockefeller University, New York, NY 10021, USA
    J Mol Biol 300:353-62. 2000
    ..The catalytic domain of primase is crescent-shaped, and the concave face of the crescent is predicted to accommodate about 10 base-pairs of RNA-DNA duplex in a loose interaction, thereby limiting processivity...
  13. ncbi Bacterial mitosis: partitioning protein ParA oscillates in spiral-shaped structures and positions plasmids at mid-cell
    Gitte Ebersbach
    Department of Biochemistry and Molecular Biology, Campusvej 55, DK 5230 Odense M, University of Southern Denmark, Denmark
    Mol Microbiol 52:385-98. 2004
    The par2 locus of Escherichia coli plasmid pB171 encodes oscillating ATPase ParA, DNA binding protein ParB and two cis-acting DNA regions to which ParB binds (parC1 and parC2)...
  14. doi ppGpp inhibits the activity of Escherichia coli DnaG primase
    Monika Maciag
    Department of Molecular Biology, University of Gdansk, Kładki 24, 80 822 Gdansk, Poland
    Plasmid 63:61-7. 2010
    ..Although in Escherichia coli such a replication regulation was not reported to date, here we show that E. coli DnaG primase is directly inhibited by ppGpp and pppGpp. However, contrary to the B...
  15. pmc The helicase-binding domain of Escherichia coli DnaG primase interacts with the highly conserved C-terminal region of single-stranded DNA-binding protein
    Natalie Naue
    Institute for Biophysical Chemistry, Hannover Medical School, Carl Neuberg Strasse 1, 30625 Hannover, Germany
    Nucleic Acids Res 41:4507-17. 2013
    During bacterial DNA replication, DnaG primase and the χ subunit of DNA polymerase III compete for binding to single-stranded DNA-binding protein (SSB), thus facilitating the switch between priming and elongation...
  16. pmc Cooperative base pair melting by helicase and polymerase positioned one nucleotide from each other
    Divya Nandakumar
    Department of Biochemistry and Molecular Biology, Rutgers Robert Wood Johnson Medical School, Piscataway, United States
    elife 4:. 2015
    Leading strand DNA synthesis requires functional coupling between replicative helicase and DNA polymerase (DNAP) enzymes, but the structural and mechanistic basis of coupling is poorly understood...
  17. pmc Migration of Escherichia coli dnaB protein on the template DNA strand as a mechanism in initiating DNA replication
    R McMacken
    Proc Natl Acad Sci U S A 74:4190-4. 1977
    ..Synthesis of RNA primers, dependent on dnaG protein (primase), occurred only on DNA that had been converted to the intermediate form...
  18. ncbi Coordinated leading- and lagging-strand synthesis at the Escherichia coli DNA replication fork. III. A polymerase-primase interaction governs primer size
    E L Zechner
    Graduate Program in Molecular Biology, Cornell University Graduate School of Medical Sciences, New York, New York 10021
    J Biol Chem 267:4054-63. 1992
    ....
  19. ncbi Coordinated leading- and lagging-strand synthesis at the Escherichia coli DNA replication fork. II. Frequency of primer synthesis and efficiency of primer utilization control Okazaki fragment size
    E L Zechner
    Graduate Program in Molecular Biology, Cornell University Graduate School of Medical Sciences, New York, New York 10021
    J Biol Chem 267:4045-53. 1992
    ..These data were used to draw general conclusions concerning the temporal order of enzymatic steps that operate during a cycle of Okazaki fragment synthesis on the lagging-strand DNA template...
  20. pmc Use of the polymerase chain reaction for physical mapping of Escherichia coli genes
    J Versalovic
    Institute for Molecular Genetics, Baylor College of Medicine, Houston, Texas
    J Bacteriol 173:5253-5. 1991
  21. doi ParAB-mediated intermolecular association of plasmid P1 parS sites
    Tranum Kaur
    School of Pharmacy, University of Waterloo, Waterloo, Ontario, Canada N2L 3G1
    Virology 421:192-201. 2011
    The P1 plasmid partition system depends on ParA-ParB proteins acting on centromere-like parS sites for a faithful plasmid segregation during the Escherichia coli cell cycle. In vivo we placed parS into host E...
  22. pmc The bacterial helicase-primase interaction: a common structural/functional module
    Panos Soultanas
    Centre for Biomolecular Sciences, School of Chemistry, University of Nottingham, Nottingham NG7 2RD, UK
    Structure 13:839-44. 2005
    ..An attractive structural model with direct biological significance for the function of this complex and also for the development of new antibacterial compounds is examined...
  23. ncbi Synthesis of polyribonucleotide chains from the 3'-hydroxyl terminus of oligodeoxynucleotides by Escherichia coli primase
    W Sun
    Biochemistry Department, New York University Medical Center, New York, New York 10016, USA
    J Biol Chem 273:16358-65. 1998
    ..We also show that the primed RNA is an effective primer for the synthesis of DNA chain by E. coli DNA polymerase III holoenzyme. The significance of this finding to primases generating multimeric length RNA is discussed...
  24. ncbi Coordinated leading- and lagging-strand synthesis at the Escherichia coli DNA replication fork. I. Multiple effectors act to modulate Okazaki fragment size
    C A Wu
    Program in Molecular Biology, Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center, New York, New York 10021
    J Biol Chem 267:4030-44. 1992
    ....
  25. ncbi Homogenous assays for Escherichia coli DnaB-stimulated DnaG primase and DnaB helicase and their use in screening for chemical inhibitors
    Yi Zhang
    Pharmaceutical Research Institute, Bristol Myers Squibb Company, Wilmington, DE 19880, USA
    Anal Biochem 304:174-9. 2002
    Escherichia coli DnaG primase is a single-stranded DNA-dependent RNA polymerase...
  26. ncbi Coordinated leading- and lagging-strand synthesis at the Escherichia coli DNA replication fork. V. Primase action regulates the cycle of Okazaki fragment synthesis
    C A Wu
    Program in Molecular Biology, Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center, New York, New York 10021
    J Biol Chem 267:4074-83. 1992
    ....
  27. ncbi Specificity of recognition sequence for Escherichia coli primase
    K Yoda
    Department of Molecular Biology, School of Science, Nagoya University, Japan
    Mol Gen Genet 227:1-8. 1991
    ..coli primase and, (iii) the consensus recognition sequence for E. coli primase associated with DnaB helicase is 3'PuPyPy 5'...
  28. ncbi DnaB helicase affects the initiation specificity of Escherichia coli primase on single-stranded DNA templates
    S Bhattacharyya
    Department of Chemistry, Center for Biotechnology, University of Nebraska Lincoln, Lincoln, Nebraska 68588 0304, USA
    Biochemistry 39:745-52. 2000
    ....
  29. ncbi DnaB helicase stimulates primer synthesis activity on short oligonucleotide templates
    S K Johnson
    Department of Chemistry, Center for Biotechnology, University of Nebraska Lincoln, Lincoln, Nebraska 68588 0304, USA
    Biochemistry 39:736-44. 2000
    ..In contrast, when primase acted alone, it stabilized the 3'-end hairpin conformation of the template so that the template's 3'-hydroxyl served as a "DNA primer" from which primase elongated to create the overlong primers...
  30. ncbi Identification of the magnesium ion binding site in the catalytic center of Escherichia coli primase by iron cleavage
    G N Godson
    Biochemistry Department, New York University Medical Center, 550 First Avenue, New York, New York 10016, USA
    Biochemistry 39:332-9. 2000
    ..Cleavage data suggested that binding to D345 is significantly stronger than to D347 and somewhat stronger than to D269...
  31. ncbi Initiation of bidirectional replication at the chromosomal origin is directed by the interaction between helicase and primase
    H Hiasa
    Molecular Biology Program, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    J Biol Chem 274:27244-8. 1999
    ....
  32. ncbi Escherichia coli primase zinc is sensitive to substrate and cofactor binding
    L Powers
    National Center for the Design of Molecular Function, Department of Electrical and Computer Engineering, Utah State University, Logan 84322 4630, USA
    Biochemistry 38:7413-20. 1999
    ..These studies clearly show that primase zinc ligation changes upon binding substrates...
  33. pmc Plasmid RSF1010 DNA replication in vitro promoted by purified RSF1010 RepA, RepB and RepC proteins
    E Scherzinger
    Max Planck Institut fur Molekulare Genetik, Abteilung Schuster, Berlin, FRG
    Nucleic Acids Res 19:1203-11. 1991
    ..The bacterial RNA polymerase, the initiation protein DnaA, and the primosomal proteins DnaB, DnaC, DnaG and DnaT are not required...
  34. pmc Characterization of mutations affecting the Escherichia coli essential GTPase era that suppress two temperature-sensitive dnaG alleles
    R A Britton
    Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas 77030, USA
    J Bacteriol 179:4575-82. 1997
    ..and reduced GTPase activity caused by the era-1 mutation suppresses dnaG2903 as well as a second dnaG allele, parB. Phenotypic analysis of the era-1 mutant at 25 degrees C showed that 10% of the cells contain four segregated ..
  35. ncbi Protein--protein interactions in the eubacterial replisome
    Patrick M Schaeffer
    Research School of Chemistry, Australian National University, Canberra, Australia
    IUBMB Life 57:5-12. 2005
    ..of the DnaA replication initiator protein, the hexameric DnaB helicase, the DnaC?helicase loading partner and the DnaG primase, leading to establishment of two replication forks...
  36. ncbi Ability of IncP-9 plasmid pM3 to replicate in Escherichia coli is dependent on both rep and par functions
    Yanina R Sevastsyanovich
    School of Biosciences, The University of Birmingham, Edgbaston, Birmingham B15 2TT, UK
    Mol Microbiol 57:819-33. 2005
    ..coli is suppressed by additional determinants from its par operon: ParB, which can be supplied in trans, and its target, the par operon promoter, required in cis to ori V-rep...
  37. ncbi Crosstalk between primase subunits can act to regulate primer synthesis in trans
    Jacob E Corn
    Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, California 94720, USA
    Mol Cell 20:391-401. 2005
    ..Our data suggest that the replisome may cooperatively use multiple primases and this conformational switch to control initiation frequency, processivity, and ultimately, Okazaki fragment synthesis...
  38. ncbi Structures of ParB bound to DNA reveal mechanism of partition complex formation
    Maria A Schumacher
    Department of Biochemistry and Molecular Biology, Oregon Health and Science University, Portland, Oregon 97239 3098, USA
    Nature 438:516-9. 2005
    ..the P1 plasmid system of Escherichia coli is a prototype comprising a partition site and two proteins, ParA and ParB. To form the partition complex necessary for segregation, P1 ParB must recognize a complicated arrangement of A-box ..
  39. ncbi Enriched sources of Escherichia coli replication proteins. The dnaG primase is a zinc metalloprotein
    N P Stamford
    Centre for Molecular Structure and Function, Research School of Chemistry, Australian National University, Canberra, ACT
    Biochim Biophys Acta 1132:17-25. 1992
    Primase, the product of the Escherichia coli dnaG gene, is the enzyme responsible for RNA primer synthesis on both template strands at replication forks during chromosomal DNA synthesis...
  40. ncbi Small molecule inhibitors of E. coli primase, a novel bacterial target
    Atul Agarwal
    Achillion Pharmaceuticals, 300 George Street, New Haven, CT 06511, USA
    Bioorg Med Chem Lett 17:2807-10. 2007
    ..This work provides a 3D pharmacophore for primase ligands, SAR trends, and leads that can be further optimized...
  41. pmc Domain swapping reveals that the C- and N-terminal domains of DnaG and DnaB, respectively, are functional homologues
    Kiran Chintakayala
    Centre for Biomolecular Sciences, School of Chemistry, University of Nottingham, University Park, Nottingham NG7 2RD, UK
    Mol Microbiol 63:1629-39. 2007
    The bacterial primase (DnaG)-helicase (DnaB) interaction is mediated by the C-terminal domain of DnaG (p16) and a linker that joins the N- and C-terminal domains (p17 and p33 respectively) of DnaB...
  42. ncbi Centromere pairing by a plasmid-encoded type I ParB protein
    Simon Ringgaard
    Department of Biochemistry and Molecular Biology, University of Southern Denmark, Campusvej 55, 5230 Odense M, Denmark
    J Biol Chem 282:28216-25. 2007
    The par2 locus of Escherichia coli plasmid pB171 encodes two trans-acting proteins, ParA and ParB, and two cis-acting sites, parC1 and parC2, to which ParB binds cooperatively...
  43. ncbi Myricetin inhibits Escherichia coli DnaB helicase but not primase
    Mark A Griep
    Department of Chemistry, University of Nebraska Lincoln, 614 Hamilton Hall, Lincoln, NE 68588 0304, USA
    Bioorg Med Chem 15:7203-8. 2007
    ..coli DnaB helicase with an inhibitory concentration at 50% maximal (IC(50)) of 11.3+/-1.6 microM. In contrast, myricetin inhibited E. coli primase at least 60-fold weaker than DnaB helicase and far weaker than any other polymerase...
  44. doi Identification of a DNA primase template tracking site redefines the geometry of primer synthesis
    Jacob E Corn
    Department of Molecular and Cell Biology, QB3 Institute, 374D Stanley Hall no 3220, University of California, Berkeley, Berkeley, California 94720, USA
    Nat Struct Mol Biol 15:163-9. 2008
    ..screening method to trap transient macromolecular interactions, we determined the structure of the Escherichia coli DnaG primase catalytic domain bound to single-stranded DNA...
  45. pmc Single-molecule studies of fork dynamics in Escherichia coli DNA replication
    Nathan A Tanner
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, 250 Longwood Avenue, Boston, Massachusetts 02115, USA
    Nat Struct Mol Biol 15:170-6. 2008
    ..5 kilobases (kb), eight-fold higher than that by Pol III alone. Addition of the primase DnaG causes a three-fold reduction in the processivity of leading-strand synthesis, an effect dependent upon the DnaB-..
  46. pmc The tri-frame model
    Elsje Pienaar
    Department of Chemical and Biomolecular Engineering, University of Nebraska, 211 Othmer Hall, Lincoln, NE 68588 0643, USA
    J Theor Biol 251:616-27. 2008
    ..The theory is demonstrated for the three genes rpsU, dnaG and rpoD of Escherichia coli, which lie on the same operon, as well as for the prfB gene.
  47. doi Staphylococcus aureus primase has higher initiation specificity, interacts with single-stranded DNA stronger, but is less stimulated by its helicase than Escherichia coli primase
    Scott A Koepsell
    Department of Microbiology and Pathology, University of Nebraska Medical Center, Omaha, NE 68198 6495, USA
    Mol Microbiol 68:1570-82. 2008
    ..coli primase. The most significant difference between the two primases was that S. aureus helicase stimulation did not broaden the S. aureus primase initiation specificity, which has important biological implications...
  48. pmc Isolation and characterization of transducing phage coding for sigma subunit of Escherichia coli RNA polymerase
    C A Gross
    Proc Natl Acad Sci U S A 76:5789-93. 1979
    ..Phage containing the HindIII fragment complement both primase (dnaG) and sigma (rpoD) whereas those containing the Sac I fragment complement only sigma...
  49. ncbi Hybrid plasmid carrying Escherichia coli genes for the primase (dnaG) and RNA polymerase sigma factor (rpoD); gene organization and control of their expression
    Y Nakamura
    Mol Gen Genet 178:487-97. 1980
  50. pmc Novel dnaG mutation in a dnaP mutant of Escherichia coli
    Y Murakami
    J Bacteriol 162:830-2. 1985
    ..coli revealed that the mutation responsible for the arrest of DNA replication and cell growth at high temperatures resides in the dnaG gene rather than in the dnaP locus as previously thought; this mutation has been designated dnaG2903.
  51. ncbi The operon that encodes the sigma subunit of RNA polymerase also encodes ribosomal protein S21 and DNA primase in E. coli K12
    Z F Burton
    Cell 32:335-49. 1983
    ..Within the sequence upstream from rpoD, we have identified the structural genes rpsU and dnaG, which encode the 30S ribosomal protein S21 and DNA primase, respectively...
  52. ncbi Promotion, termination, and anti-termination in the rpsU-dnaG-rpoD macromolecular synthesis operon of E. coli K-12
    J R Lupski
    Mol Gen Genet 195:391-401. 1984
    The regulatory regions for the rpsU-dnaG-rpoD macromolecular synthesis operon have been fused to a structural gene whose product is readily assayed (the Cmr structural gene coding for chloramphenicol acetyl transferase, CAT)...
  53. pmc Regulation of expression of the Escherichia coli dnaG gene and amplification of the dnaG primase
    M S Wold
    Proc Natl Acad Sci U S A 79:4907-11. 1982
    We have isolated lambda transducing phages carrying the Escherichia coli primase gene (dnaG) and mapped restriction sites in the cloned bacterial DNA segments...
  54. ncbi Regulation of the rpsU-dnaG-rpoD macromolecular synthesis operon and the initiation of DNA replication in Escherichia coli K-12
    J R Lupski
    Mol Gen Genet 189:48-57. 1983
    We have fused the E. coli dnaG 5' regulatory region to the TcR structural gene in the promoter probe plasmids pPV33 and pKK175-6 to demonstrate that a promoter activity is located on a 250 bp SacII-HindIII restriction fragment and that a ..
  55. ncbi Cloning and characterization of the Escherichia coli chromosomal region surrounding the dnaG Gene, with a correlated physical and genetic map of dnaG generated via transposon Tn5 mutagenesis
    J R Lupski
    Mol Gen Genet 185:120-8. 1982
    A 24 kilobase pair region of the E. coli chromosome surrounding the dnaG gene has been cloned and characterized. A lambda phage library was constructed by ligating a Sau3A( decreases GATC) partial DNA digest of the entire E...
  56. ncbi Transcription from a heat-inducible promoter causes heat shock regulation of the sigma subunit of E. coli RNA polymerase
    W E Taylor
    Cell 38:371-81. 1984
    The rpoD gene encoding the sigma subunit of E. coli RNA polymerase is cotranscribed with rpsU and dnaG, encoding ribosomal protein S21 and DNA primase, respectively...
  57. pmc Sequences of the Escherichia coli dnaG primase gene and regulation of its expression
    B L Smiley
    Proc Natl Acad Sci U S A 79:4550-4. 1982
    The nucleotide sequence of a cloned section of the Escherichia coli chromosome containing the dnaG primase gene [Lupski, J., Smiley, B., Blattner, F. & Godson, G. N. (1982) Mol. Gen, Genet. 185, 120--128] has been determined...
  58. ncbi Studies of the functional topography of the catalytic center of Escherichia coli primase
    A A Mustaev
    Public Health Research Institute, New York, New York 10016, USA
    J Biol Chem 270:15711-8. 1995
    ..When cross-linking was performed on the preformed primase/SSB/G4oric complex with long arm reagents (12-15 A), SSB was also labeled, indicating a close proximity to the site of pRNA synthesis...
  59. ncbi Primase couples leading- and lagging-strand DNA synthesis from oriC
    H Hiasa
    Program in Molecular Biology, Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center, New York, New York 10021
    J Biol Chem 269:6058-63. 1994
    ..On the other hand, high concentrations of primase resulted in successful capture of the helicase leading to the formation at oriC of coupled replication forks capable of coordinated leading- and lagging-strand synthesis...
  60. ncbi An Escherichia coli cis-acting antiterminator sequence: the dnaG nut site
    N Almond
    Biochemistry Department, NYU Medical Center, NY 10016
    Mol Gen Genet 216:195-203. 1989
    The Escherichia coli rpsU-dnaG-rpoD operon contains an internal transcription terminator T1 located in the intergenic region between the rpsU and dnaG genes (Smiley et al. 1982)...
  61. pmc DNA replication termination in Escherichia coli parB (a dnaG allele), parA, and gyrB mutants affected in DNA distribution
    V Norris
    J Bacteriol 168:494-504. 1986
    We investigated the Escherichia coli mutants carrying the parB, parA, and gyrB mutations, all of which display faulty chromosome partitioning at the nonpermissive temperature, to see whether their phenotype reflected a defect in the ..
  62. ncbi The tyrosine photophysics of a primase-derived peptide are sensitive to the peptide's zinc-bound state: proof that the bacterial primase hypothetical zinc finger sequence binds zinc
    M A Griep
    Department of Chemistry, University of Nebraska, Lincoln 68588 0304, USA
    Biochemistry 36:544-53. 1997
    ....
  63. ncbi Leading strand synthesis of R1 plasmid replication in vitro is primed by primase alone at a specific site downstream of oriR
    H Masai
    Department of Molecular Biology, Institute of Molecular and Cellular Biology, Palo Alto, California 94304
    J Biol Chem 264:8082-90. 1989
    ..chimeric single-stranded phage could be replicated in vitro with only single-stranded DNA binding protein, primase (dnaG gene product), and DNA polymerase III holoenzyme...
  64. ncbi The role of zinc and the reactivity of cysteines in Escherichia coli primase
    M A Griep
    Department of Chemistry, University of Nebraska, Lincoln 68588 0304, USA
    Biochemistry 35:8260-7. 1996
    ..Zinc could be prevented from binding to the weak site by the presence of magnesium, indicating that the weak site may be the catalytic magnesium site in which two of the seven cysteines were located...
  65. ncbi The ordered assembly of the phiX174-type primosome. II. Preservation of primosome composition from assembly through replication
    J Y Ng
    Graduate Program in Molecular Biology, Cornell University Graduate School of Medical Sciences, New York, New York 10021, USA
    J Biol Chem 271:15649-55. 1996
    ..DNA-binding protein-coated phiX174 DNA by the combination of PriA, PriB, PriC, DnaT, DnaB, DnaC, and DnaG. The presence and relative amounts of primosomal proteins in these complexes were determined by Western blotting...
  66. pmc Genetic suppression of a dnaG mutation in Escherichia coli
    T Katayama
    Institute for Virus Research, Kyoto University, Japan
    J Bacteriol 171:1485-91. 1989
    ..They fall into two classes. The sdgA mutations were genetically mapped very close to and upstream of the dnaG gene and were found to be cis dominant...
  67. ncbi Magnesium acetate induces a conformational change in Escherichia coli primase
    T M Urlacher
    Department of Chemistry, University of Nebraska Lincoln 68588 0304, USA
    Biochemistry 34:16708-14. 1995
    ..The data were best fit to a two-state model in which one conformation had a high affinity for magnesium, KR = 83.4 M-1, and the other state had virtually no affinity...
  68. ncbi Primer synthesis kinetics by Escherichia coli primase on single-stranded DNA templates
    J R Swart
    Department of Chemistry, University of Nebraska, Lincoln 68588 0304, USA
    Biochemistry 34:16097-106. 1995
    ..Nevertheless, the overlong primers did appear to arise as a consequence of the template length-dependent species since their length was double and they arose in the time course after the length-dependent species...
  69. ncbi Primase from Escherichia coli primes single-stranded templates in the absence of single-stranded DNA-binding protein or other auxiliary proteins. Template sequence requirements based on the bacteriophage G4 complementary strand origin and Okazaki fragment
    J R Swart
    Department of Chemistry, University of Nebraska, Lincoln 68588 0304
    J Biol Chem 268:12970-6. 1993
    ..We also report primase's ability to incorporate dideoxyribonucleotide triphosphates into primers, which has allowed us to determine directly both length and sequence of primers synthesized...
  70. pmc Comparative analysis of functional and structural features in the primase-dependent priming signals, G sites, from phages and plasmids
    K Tanaka
    Department of Agricultural Chemistry, Kyoto University, Japan
    J Bacteriol 176:3606-13. 1994
    The primase-dependent priming signals, G sites, are directly recognized by the Escherichia coli primase (dnaG gene product) and conduct the synthesis of primer RNAs...
  71. pmc Are minichromosomes valid model systems for DNA replication control? Lessons learned from Escherichia coli
    T Asai
    Department of Cell Biology, University of New Mexico Health Sciences Center, Albuquerque 87131, USA
    Mol Microbiol 29:671-5. 1998
    ..The results indicate that requirements for initiation at the chromosomal origin, oriC, are drastically different from those for initiation at cloned oriC...
  72. ncbi Intragenic localization of amber and temperature-sensitive rpoD mutations affecting RNA polymerase sigma factor of Escherichia coli
    Y Nakamura
    Mol Gen Genet 189:193-8. 1983
    ..It is suggested that the enhanced synthesis of incomplete sigma polypeptides encoded by rpoD47 or rpoD63 phenotypically suppresses the defects in sigma function at least partially...
  73. ncbi Structure of Escherichia coli dnaC. Identification of a cysteine residue possibly involved in association with dnaB protein
    N Nakayama
    J Biol Chem 262:10475-80. 1987
    ..Similar to dnaG and dnaT, dnaC uses several minor codons; the significance of these minor codons to the low level expression of the ..
  74. pmc Role of conserved amino acids in the catalytic activity of Escherichia coli primase
    Anna Rodina
    Biochemistry Department, New York University Medical School, NY 10016, USA
    J Bacteriol 188:3614-21. 2006
    ..From these data we discuss the significant step of the 2-nucleotide primer RNA synthesis by E. coli primase and the role of amino acids Lys229, Tyr267, and Met268 in primase complex stability...
  75. ncbi Mechanism and stoichiometry of interaction of DnaG primase with DnaB helicase of Escherichia coli in RNA primer synthesis
    Atanaska V Mitkova
    Department of Molecular Biology, School of Osteopathic Medicine, University of Medicine and Dentistry of New Jersey, Stratford, New Jersey 08084, USA
    J Biol Chem 278:52253-61. 2003
    ..strand of the replication fork in Escherichia coli requires the replicative DnaB helicase and the DNA primase, the DnaG gene product...
  76. ncbi Structure of the Escherichia coli primase/single-strand DNA-binding protein/phage G4oric complex required for primer RNA synthesis
    W Sun
    Biochemistry Department, New York University Medical Center, 550 First Avenue, New York 10016, USA
    J Mol Biol 276:689-703. 1998
    ..Novel techniques used in this study include antisense oligonucleotide protection and RNA synthesis on an SSB-melted, double-stranded DNA template...
  77. ncbi Missense mutations in the 3' end of the Escherichia coli dnaG gene do not abolish primase activity but do confer the chromosome-segregation-defective (par) phenotype
    J Versalovic
    Department of Molecular and Human Genetics, Baylor College of Medicine, Houston TX 77030, USA
    Microbiology 143:585-94. 1997
    Isogenic dnaG strains of Escherichia coli with the parB and dnaG2903 alleles in the MG1655 chromosomal background displayed the classic par phenotype at the nonpermissive temperature of 42 degrees C...