dacD

Summary

Gene Symbol: dacD
Description: D-alanyl-D-alanine carboxypeptidase; penicillin-binding protein 6b
Alias: ECK2004, JW5329, phsE, phsF, yeeC
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. doi Moderate deacylation efficiency of DacD explains its ability to partially restore beta-lactam resistance in Escherichia coli PBP5 mutant
    Chiranjit Chowdhury
    Department of Biotechnology, Indian Institute of Technology, Kharagpur, West Bengal, India
    FEMS Microbiol Lett 337:73-80. 2012
  2. doi Physiological functions of D-alanine carboxypeptidases in Escherichia coli
    Anindya S Ghosh
    Department of Biotechnology, Indian Institute of Technology, Kharagpur, District West Midnapore, West Bengal, Pin 721302, India
    Trends Microbiol 16:309-17. 2008
  3. pmc Oxygen limitation modulates pH regulation of catabolism and hydrogenases, multidrug transporters, and envelope composition in Escherichia coli K-12
    Everett T Hayes
    Department of Biology, Kenyon College, Gambier, OH 43022, USA
    BMC Microbiol 6:89. 2006
  4. pmc Daughter cell separation by penicillin-binding proteins and peptidoglycan amidases in Escherichia coli
    Richa Priyadarshini
    Department of Microbiology and Immunology, University of North Dakota, Grand Forks, ND 58202 9037, USA
    J Bacteriol 188:5345-55. 2006
  5. pmc Bacterial cell wall synthesis: new insights from localization studies
    Dirk Jan Scheffers
    Department of Molecular Microbiology, Institute of Molecular Cell Biology, Vrije Universiteit, De Boelelaan 1085, 1081 HV Amsterdam, The Netherlands
    Microbiol Mol Biol Rev 69:585-607. 2005
  6. pmc Endopeptidase penicillin-binding proteins 4 and 7 play auxiliary roles in determining uniform morphology of Escherichia coli
    Bernadette M Meberg
    Department of Microbiology and Immunology, University of North Dakota School of Medicine, Grand Forks, ND 58202 9037, USA
    J Bacteriol 186:8326-36. 2004
  7. pmc FtsZ collaborates with penicillin binding proteins to generate bacterial cell shape in Escherichia coli
    Archana Varma
    Department of Microbiology and Immunology, University of North Dakota School of Medicine and Health Sciences, Grand Forks, ND 58202, USA
    J Bacteriol 186:6768-74. 2004
  8. ncbi Bacterial shape
    Kevin D Young
    Department of Microbiology and Immunology, University of North Dakota School of Medicine, Grand Forks 58202 9037, USA
    Mol Microbiol 49:571-80. 2003
  9. pmc Sequences near the active site in chimeric penicillin binding proteins 5 and 6 affect uniform morphology of Escherichia coli
    Anindya S Ghosh
    Department of Microbiology and Immunology, University of North Dakota School of Medicine, Grand Forks, North Dakota 58202 9037, USA
    J Bacteriol 185:2178-86. 2003
  10. pmc Branching of Escherichia coli cells arises from multiple sites of inert peptidoglycan
    Miguel A de Pedro
    Centro de Biologia Molecular Severo Ochoa, Consejo Superior de Investigaciones Cientificas, Universidad Autonoma de Madrid, Campus de Cantoblanco, 28049 Madrid, Spain
    J Bacteriol 185:1147-52. 2003

Scientific Experts

  • K D Young
  • Miguel A de Pedro
  • J V Holtje
  • Everett T Hayes
  • Dirk Jan Scheffers
  • Anindya S Ghosh
  • Chiranjit Chowdhury
  • Richa Priyadarshini
  • David E Nelson
  • D E Nelson
  • Bernadette M Meberg
  • Archana Varma
  • Avery L Paulson
  • Frederick Harris
  • Debasish Kar
  • Mouparna Dutta
  • Akash Kumar
  • S A Denome
  • David L Popham
  • M R Baquero
  • Ulrich Seydel
  • Klaus Brandenburg
  • David Phoenix
  • T A Henderson
  • P K Elf
  • F Moreno
  • J A Ayala
  • J C Quintela
  • M Bouzon

Detail Information

Publications16

  1. doi Moderate deacylation efficiency of DacD explains its ability to partially restore beta-lactam resistance in Escherichia coli PBP5 mutant
    Chiranjit Chowdhury
    Department of Biotechnology, Indian Institute of Technology, Kharagpur, West Bengal, India
    FEMS Microbiol Lett 337:73-80. 2012
    ..b>DacD can partially compensate for the lost beta-lactam resistance in PBP5 mutant, although its biochemical reason is ..
  2. doi Physiological functions of D-alanine carboxypeptidases in Escherichia coli
    Anindya S Ghosh
    Department of Biotechnology, Indian Institute of Technology, Kharagpur, District West Midnapore, West Bengal, Pin 721302, India
    Trends Microbiol 16:309-17. 2008
    ..Several low molecular mass PBPs including PBP4, PBP5, PBP6 and DacD seem to possess DD-carboxypeptidase (DD-CPase) activity, but these proteins are dispensable for survival in ..
  3. pmc Oxygen limitation modulates pH regulation of catabolism and hydrogenases, multidrug transporters, and envelope composition in Escherichia coli K-12
    Everett T Hayes
    Department of Biology, Kenyon College, Gambier, OH 43022, USA
    BMC Microbiol 6:89. 2006
    ..Many pH-dependent genes are co-regulated by anaerobiosis, but the overall intersection of pH stress and oxygen limitation has not been investigated...
  4. pmc Daughter cell separation by penicillin-binding proteins and peptidoglycan amidases in Escherichia coli
    Richa Priyadarshini
    Department of Microbiology and Immunology, University of North Dakota, Grand Forks, ND 58202 9037, USA
    J Bacteriol 188:5345-55. 2006
    ..Unexpectedly, amidase mutants lacking PBP 5 grew in long twisted chains instead of straight filaments, indicating that overall septal morphology was also defective in these strains...
  5. pmc Bacterial cell wall synthesis: new insights from localization studies
    Dirk Jan Scheffers
    Department of Molecular Microbiology, Institute of Molecular Cell Biology, Vrije Universiteit, De Boelelaan 1085, 1081 HV Amsterdam, The Netherlands
    Microbiol Mol Biol Rev 69:585-607. 2005
    ..We discuss a model in which, at least for a subset of PBPs, the presence of substrate is a major factor in determining PBP localization...
  6. pmc Endopeptidase penicillin-binding proteins 4 and 7 play auxiliary roles in determining uniform morphology of Escherichia coli
    Bernadette M Meberg
    Department of Microbiology and Immunology, University of North Dakota School of Medicine, Grand Forks, ND 58202 9037, USA
    J Bacteriol 186:8326-36. 2004
    ..In addition, the combined loss of three DD-carboxypeptidases, PBPs 5 and 6 and DacD, also impaired cell shape...
  7. pmc FtsZ collaborates with penicillin binding proteins to generate bacterial cell shape in Escherichia coli
    Archana Varma
    Department of Microbiology and Immunology, University of North Dakota School of Medicine and Health Sciences, Grand Forks, ND 58202, USA
    J Bacteriol 186:6768-74. 2004
    ....
  8. ncbi Bacterial shape
    Kevin D Young
    Department of Microbiology and Immunology, University of North Dakota School of Medicine, Grand Forks 58202 9037, USA
    Mol Microbiol 49:571-80. 2003
    ....
  9. pmc Sequences near the active site in chimeric penicillin binding proteins 5 and 6 affect uniform morphology of Escherichia coli
    Anindya S Ghosh
    Department of Microbiology and Immunology, University of North Dakota School of Medicine, Grand Forks, North Dakota 58202 9037, USA
    J Bacteriol 185:2178-86. 2003
    ..coli PBP 5...
  10. pmc Branching of Escherichia coli cells arises from multiple sites of inert peptidoglycan
    Miguel A de Pedro
    Centro de Biologia Molecular Severo Ochoa, Consejo Superior de Investigaciones Cientificas, Universidad Autonoma de Madrid, Campus de Cantoblanco, 28049 Madrid, Spain
    J Bacteriol 185:1147-52. 2003
    ..These observations suggest that in dacA mutants, branches and other morphological anomalies may arise from split polar caps or by de novo generation of new poles built around inert peptidoglycan patches in the side walls of the cell...
  11. ncbi Investigations into the mechanisms used by the C-terminal anchors of Escherichia coli penicillin-binding proteins 4, 5, 6 and 6b for membrane interaction
    Frederick Harris
    Department of Forensic and Investigative Science, University of Central Lancashire, Preston, UK
    Eur J Biochem 269:5821-9. 2002
    ....
  12. pmc Contribution of membrane-binding and enzymatic domains of penicillin binding protein 5 to maintenance of uniform cellular morphology of Escherichia coli
    David E Nelson
    Department of Microbiology and Immunology, University of North Dakota School of Medicine, Grand Forks, North Dakota 58202 9037, USA
    J Bacteriol 184:3630-9. 2002
    ..PBPs) of Escherichia coli are closely related and have similar DD-carboxypeptidase activities (PBPs 4, 5, and 6 and DacD). However, only one, PBP 5, has a demonstrated physiological function. In its absence, certain mutants of E...
  13. pmc Contributions of PBP 5 and DD-carboxypeptidase penicillin binding proteins to maintenance of cell shape in Escherichia coli
    D E Nelson
    Department of Microbiology and Immunology, University of North Dakota School of Medicine and Health Sciences, Grand Forks, ND 58202 9037, USA
    J Bacteriol 183:3055-64. 2001
    Escherichia coli has 12 recognized penicillin binding proteins (PBPs), four of which (PBPs 4, 5, and 6 and DacD) have DD-carboxypeptidase activity...
  14. pmc Escherichia coli mutants lacking all possible combinations of eight penicillin binding proteins: viability, characteristics, and implications for peptidoglycan synthesis
    S A Denome
    Department of Microbiology and Immunology, School of Medicine, University of North Dakota, Grand Forks, North Dakota 58202 9037, USA
    J Bacteriol 181:3981-93. 1999
    ..In addition, the dacD gene was deleted from two septuple mutants, creating strains lacking eight genes...
  15. pmc Growth of the stress-bearing and shape-maintaining murein sacculus of Escherichia coli
    J V Holtje
    Max Planck Institut fur Entwicklungsbiologie, Abteilung Biochemie, Tubingen, Germany
    Microbiol Mol Biol Rev 62:181-203. 1998
    ..A model is presented that postulates that maintenance of bacterial shape is achieved by the enzyme complex copying the preexisting murein sacculus that plays the role of a template...
  16. pmc dacD, an Escherichia coli gene encoding a novel penicillin-binding protein (PBP6b) with DD-carboxypeptidase activity
    M R Baquero
    Unidad de Genetica Molecular, Hospital Ramon y Cajal, Madrid, Spain
    J Bacteriol 178:7106-11. 1996
    ..Therefore, we called the protein PBP6b and the gene dacD. As with other E. coli DD-carboxypeptidases, PBP6b is not essential for cell growth...