dacB

Summary

Gene Symbol: dacB
Description: D-alanyl-D-alanine carboxypeptidase
Alias: ECK3171, JW3149
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. pmc Thermosensitive mutation in Escherichia coli simultaneously causing defects in penicillin-binding protein-1Bs and in enzyme activity for peptidoglycan synthesis in vitro
    S Tamaki
    Proc Natl Acad Sci U S A 74:5472-6. 1977
  2. pmc Penicillin-binding protein 7 and its relationship to lysis of nongrowing Escherichia coli
    E Tuomanen
    Rockefeller University, New York, New York 10021
    J Bacteriol 169:4912-5. 1987
  3. pmc Penicillin-binding proteins and induction of AmpC beta-lactamase
    C C Sanders
    Center for Research in Anti Infectives and Biotechnology, Department of Medical Microbiology, Creighton University School of Medicine, Omaha, Nebraska 68178, USA
    Antimicrob Agents Chemother 41:2013-5. 1997
  4. pmc Unequal distribution of penicillin-binding proteins among inner membrane vesicles of Escherichia coli
    G H Jacoby
    Department of Microbiology and Immunology, University of North Dakota School of Medicine, Grand Forks 58202
    J Bacteriol 170:3660-7. 1988
  5. ncbi Crystal structure of penicillin binding protein 4 (dacB) from Escherichia coli, both in the native form and covalently linked to various antibiotics
    Hiroyuki Kishida
    Protein Design Laboratory, Yokohama City University, Suehiro 1 7 29, Tsurumi ku, Yokohama 230 0045, Japan
    Biochemistry 45:783-92. 2006
  6. pmc Identification and characterization of the Escherichia coli gene dsbB, whose product is involved in the formation of disulfide bonds in vivo
    D Missiakas
    Department of Cellular, Viral and Molecular Biology, University of Utah School of Medicine, Salt Lake City 84132
    Proc Natl Acad Sci U S A 90:7084-8. 1993
  7. pmc Multimodular penicillin-binding proteins: an enigmatic family of orthologs and paralogs
    C Goffin
    Centre d Ingénierie des Protéines, Universite de Liege, Institut de Chimie, B 4000 Sart Tilman Liège, Belgium
    Microbiol Mol Biol Rev 62:1079-93. 1998
  8. ncbi An investigation into the lipid interactions of peptides corresponding to the C-terminal anchoring domains of Escherichia coli penicillin-binding proteins 4, 5 and 6
    F Harris
    Department of Applied Biology, University of Central Lancashire, Preston PR1 2HE, UK
    Biochim Biophys Acta 1415:10-22. 1998
  9. ncbi Transcriptional analysis of the restriction and modification genes of bacteriophage P1
    A D Sharrocks
    Department of Molecular Biology, Krebs Institute, University of Sheffield, UK
    Mol Microbiol 5:685-94. 1991
  10. pmc Penicillin binding protein 5 affects cell diameter, contour, and morphology of Escherichia coli
    D E Nelson
    Department of Microbiology and Immunology, School of Medicine, University of North Dakota, Grand Forks, North Dakota 58202 9037, USA
    J Bacteriol 182:1714-21. 2000

Scientific Experts

  • K D Young
  • Miguel A de Pedro
  • J V Holtje
  • Dirk Jan Scheffers
  • N Nanninga
  • Thomas B Clarke
  • Anindya S Ghosh
  • D E Nelson
  • Richa Priyadarshini
  • David E Nelson
  • Adeline Derouaux
  • Mee Jung Han
  • Daniel Vega
  • Hiroyuki Kishida
  • F Harris
  • Moritz von Rechenberg
  • Claude V Gallant
  • Archana Varma
  • Bernadette M Meberg
  • Ute Meisel
  • Avery L Paulson
  • Christian Eberhardt
  • Frederick Harris
  • Christoph Heidrich
  • Colette Goffin
  • B M Meberg
  • W Vollmer
  • D A Phoenix
  • Martine Nguyen-Distèche
  • Eefjan Breukink
  • S A Denome
  • Mohammed Terrak
  • Claudine Fraipont
  • Benoît Wolf
  • Chiranjit Chowdhury
  • T A Henderson
  • F Fusetti
  • Amy L Melquist
  • Jeremy R H Tame
  • Satoru Unzai
  • Adrian Lloyd
  • S W Lazar
  • David I Roper
  • C Goffin
  • Sam Yong Park
  • David L Popham
  • Juan A Ayala
  • Sang Yup Lee
  • Cindy Lou Chepanoske
  • Lakshmi P Kotra
  • Lori L Burrows
  • Bonnie E Richardson
  • Jacqueline M Leung
  • Yuejun Zhen
  • Craig Daniels
  • Brian Kelly Blake
  • Yew Seng J Ho
  • Vladimir Kery
  • Nafei Xu
  • C C Sanders
  • Lars Kuerschner
  • David S Weiss
  • Waldemar Vollmer
  • Jean Marie Ghuysen
  • M R Baquero
  • Klaus Brandenburg
  • Ulrich Seydel
  • Astrid Ursinus
  • Heinz Schwarz
  • David Phoenix
  • Jürgen Berger
  • F C Sailer
  • B W Dijkstra
  • M M Thunnissen
  • P K Elf
  • H Mottl
  • W Keck
  • E B Simpson
  • J R Gittins
  • A Tormo
  • J M Ghuysen
  • W Obermann
  • R Demel
  • B De Kruijff
  • R Kolter
  • M Almiron
  • M Matsuhashi
  • P A Bradford
  • A F Ehrhardt
  • W E Sanders

Detail Information

Publications76

  1. pmc Thermosensitive mutation in Escherichia coli simultaneously causing defects in penicillin-binding protein-1Bs and in enzyme activity for peptidoglycan synthesis in vitro
    S Tamaki
    Proc Natl Acad Sci U S A 74:5472-6. 1977
    ..Possible functions of some other penicillin-binding proteins in compensating for lack of protein-1Bs were also proposed...
  2. pmc Penicillin-binding protein 7 and its relationship to lysis of nongrowing Escherichia coli
    E Tuomanen
    Rockefeller University, New York, New York 10021
    J Bacteriol 169:4912-5. 1987
    ..A striking correlation existed in drugs between capability of lysing amino acid-starved (nongrowing) E. coli and binding to PBP 7. The findings suggest that PBP 7 is a new, physiologically significant target for beta-lactam antibiotics...
  3. pmc Penicillin-binding proteins and induction of AmpC beta-lactamase
    C C Sanders
    Center for Research in Anti Infectives and Biotechnology, Department of Medical Microbiology, Creighton University School of Medicine, Omaha, Nebraska 68178, USA
    Antimicrob Agents Chemother 41:2013-5. 1997
    ..Loss of PBP 4 resulted in diminished inducibility. This suggests that if PBPs are involved in induction of AmpC beta-lactamase, there is probably a redundancy in function among the different PBPs...
  4. pmc Unequal distribution of penicillin-binding proteins among inner membrane vesicles of Escherichia coli
    G H Jacoby
    Department of Microbiology and Immunology, University of North Dakota School of Medicine, Grand Forks 58202
    J Bacteriol 170:3660-7. 1988
    ..In addition, in cell lysates and in vesicle fractions, a 60,000-dalton aztreonam-insensitive PBP or protein fragment was observed which could potentially be confused with PBP3...
  5. ncbi Crystal structure of penicillin binding protein 4 (dacB) from Escherichia coli, both in the native form and covalently linked to various antibiotics
    Hiroyuki Kishida
    Protein Design Laboratory, Yokohama City University, Suehiro 1 7 29, Tsurumi ku, Yokohama 230 0045, Japan
    Biochemistry 45:783-92. 2006
    ..The altered geometry of conserved active site residues compared with those of other PBPs suggests a possible cause for the slow deacylation rate of PBP4...
  6. pmc Identification and characterization of the Escherichia coli gene dsbB, whose product is involved in the formation of disulfide bonds in vivo
    D Missiakas
    Department of Cellular, Viral and Molecular Biology, University of Utah School of Medicine, Salt Lake City 84132
    Proc Natl Acad Sci U S A 90:7084-8. 1993
    ..The dsbB gene maps at 26.5 min on the genetic map of the E. coli chromosome, and its transcription is directed from two promoters, neither of which resembles the canonical E sigma 70-recognized promoter...
  7. pmc Multimodular penicillin-binding proteins: an enigmatic family of orthologs and paralogs
    C Goffin
    Centre d Ingénierie des Protéines, Universite de Liege, Institut de Chimie, B 4000 Sart Tilman Liège, Belgium
    Microbiol Mol Biol Rev 62:1079-93. 1998
    ..Studies of the reaction pathways by using the methods of quantum chemistry suggest that resistance to penicillin is a road of no return...
  8. ncbi An investigation into the lipid interactions of peptides corresponding to the C-terminal anchoring domains of Escherichia coli penicillin-binding proteins 4, 5 and 6
    F Harris
    Department of Applied Biology, University of Central Lancashire, Preston PR1 2HE, UK
    Biochim Biophys Acta 1415:10-22. 1998
    ..Furthermore, we have speculated that the presence of cationic residues in the hydrophilic face of these alpha-helices may help facilitate membrane interaction...
  9. ncbi Transcriptional analysis of the restriction and modification genes of bacteriophage P1
    A D Sharrocks
    Department of Molecular Biology, Krebs Institute, University of Sheffield, UK
    Mol Microbiol 5:685-94. 1991
    ..In addition a functional promoter is located on the antisense strand close to the res promoter region. The relationship between the transcription units of the res and mod genes is discussed...
  10. pmc Penicillin binding protein 5 affects cell diameter, contour, and morphology of Escherichia coli
    D E Nelson
    Department of Microbiology and Immunology, School of Medicine, University of North Dakota, Grand Forks, North Dakota 58202 9037, USA
    J Bacteriol 182:1714-21. 2000
    ..We conclude that among the low-molecular-weight PBPs, PBP 5 plays a principle role in determining cell diameter, surface uniformity, and overall topology of the peptidoglycan sacculus...
  11. pmc Reconstruction of Escherichia coli mrcA (PBP 1a) mutants lacking multiple combinations of penicillin binding proteins
    B M Meberg
    Department of Microbiology and Immunology, University of North Dakota School of Medicine, Grand Forks, North Dakota 58202 9037, USA
    J Bacteriol 183:6148-9. 2001
    ....
  12. pmc Contributions of PBP 5 and DD-carboxypeptidase penicillin binding proteins to maintenance of cell shape in Escherichia coli
    D E Nelson
    Department of Microbiology and Immunology, University of North Dakota School of Medicine and Health Sciences, Grand Forks, ND 58202 9037, USA
    J Bacteriol 183:3055-64. 2001
    ..coli...
  13. ncbi Approaching the physiological functions of penicillin-binding proteins in Escherichia coli
    K D Young
    Department of Microbiology and Immunology, School of Medicine and Health Sciences, University of North Dakota, Grand Forks 58202 9037, USA
    Biochimie 83:99-102. 2001
    ....
  14. pmc A simple screen for murein transglycosylase inhibitors
    W Vollmer
    Max Planck Institut fur Entwicklungsbiologie, Abteilung Biochemie, 72076 Tubingen, Germany
    Antimicrob Agents Chemother 44:1181-5. 2000
    ..Crude membrane extracts can be used as a source for the PBPs, and different kinds of labels for the penicillin-PBP complexes can be used. The assay can be adapted to high-throughput screens...
  15. ncbi Investigations into the mechanisms used by the C-terminal anchors of Escherichia coli penicillin-binding proteins 4, 5, 6 and 6b for membrane interaction
    Frederick Harris
    Department of Forensic and Investigative Science, University of Central Lancashire, Preston, UK
    Eur J Biochem 269:5821-9. 2002
    ....
  16. pmc Escherichia coli mutants lacking all possible combinations of eight penicillin binding proteins: viability, characteristics, and implications for peptidoglycan synthesis
    S A Denome
    Department of Microbiology and Immunology, School of Medicine, University of North Dakota, Grand Forks, North Dakota 58202 9037, USA
    J Bacteriol 181:3981-93. 1999
    ..In addition, members of the set of PBP mutants will provide excellent starting points for answering fundamental questions about other aspects of cell wall metabolism...
  17. pmc Role of the Escherichia coli SurA protein in stationary-phase survival
    S W Lazar
    Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Bacteriol 180:5704-11. 1998
    ..Cells lacking penicillin binding protein 3 and sigmaS had a survival defect similar to that of surA rpoS double mutants, suggesting that SurA foldase activity is important for the proper assembly of the cell wall-synthesizing apparatus...
  18. pmc Growth of the stress-bearing and shape-maintaining murein sacculus of Escherichia coli
    J V Holtje
    Max Planck Institut fur Entwicklungsbiologie, Abteilung Biochemie, Tubingen, Germany
    Microbiol Mol Biol Rev 62:181-203. 1998
    ..A model is presented that postulates that maintenance of bacterial shape is achieved by the enzyme complex copying the preexisting murein sacculus that plays the role of a template...
  19. pmc Morphogenesis of Escherichia coli
    N Nanninga
    Institute for Molecular Cell Biology, BioCentrum Amsterdam, University of Amsterdam, The Netherlands
    Microbiol Mol Biol Rev 62:110-29. 1998
    ..An attempt has been made to show how this dynamic relationship during the cell cycle affects cell polarity and how it leads to cell division...
  20. pmc Endopeptidase penicillin-binding proteins 4 and 7 play auxiliary roles in determining uniform morphology of Escherichia coli
    Bernadette M Meberg
    Department of Microbiology and Immunology, University of North Dakota School of Medicine, Grand Forks, ND 58202 9037, USA
    J Bacteriol 186:8326-36. 2004
    ....
  21. doi Mutational analysis of the substrate specificity of Escherichia coli penicillin binding protein 4
    Thomas B Clarke
    Department of Biological Sciences, University of Warwick, Coventry, UK
    Biochemistry 48:2675-83. 2009
    ..This provides the first direct demonstration of the importance of residues forming a subsite to accommodate meso-2,6-diaminopimelic acid in both the DD-carboxypeptidase and DD-endopeptidase activities of a class C LMM-PBP...
  22. doi Physiological functions of D-alanine carboxypeptidases in Escherichia coli
    Anindya S Ghosh
    Department of Biotechnology, Indian Institute of Technology, Kharagpur, District West Midnapore, West Bengal, Pin 721302, India
    Trends Microbiol 16:309-17. 2008
    ....
  23. pmc The monofunctional glycosyltransferase of Escherichia coli localizes to the cell division site and interacts with penicillin-binding protein 3, FtsW, and FtsN
    Adeline Derouaux
    Centre d Ingénierie des Protéines, Universite de Liege, Institut de Chimie, B6a, B 4000 Sart Tilman Liège, Belgium
    J Bacteriol 190:1831-4. 2008
    ....
  24. pmc Daughter cell separation by penicillin-binding proteins and peptidoglycan amidases in Escherichia coli
    Richa Priyadarshini
    Department of Microbiology and Immunology, University of North Dakota, Grand Forks, ND 58202 9037, USA
    J Bacteriol 188:5345-55. 2006
    ..Unexpectedly, amidase mutants lacking PBP 5 grew in long twisted chains instead of straight filaments, indicating that overall septal morphology was also defective in these strains...
  25. pmc The Escherichia coli proteome: past, present, and future prospects
    Mee Jung Han
    Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology, Yuseong Gu, Daejeon 305 701, Republic of Korea
    Microbiol Mol Biol Rev 70:362-439. 2006
    ..Here, we review the past development and current status of E. coli proteome research in terms of its biological, biotechnological, and methodological significance and suggest future prospects...
  26. ncbi The DD-carboxypeptidase activity encoded by pbp4B is not essential for the cell growth of Escherichia coli
    Daniel Vega
    Centro de Biologia Molecular Severo Ochoa CSIC UAM, Campus de Cantoblanco, 28049, Madrid, Spain
    Arch Microbiol 185:23-7. 2006
    ..The penicillin-binding capacity and a low DD-carboxypeptidase activity of PBP4B are demonstrated...
  27. pmc Bacterial cell wall synthesis: new insights from localization studies
    Dirk Jan Scheffers
    Department of Molecular Microbiology, Institute of Molecular Cell Biology, Vrije Universiteit, De Boelelaan 1085, 1081 HV Amsterdam, The Netherlands
    Microbiol Mol Biol Rev 69:585-607. 2005
    ..We discuss a model in which, at least for a subset of PBPs, the presence of substrate is a major factor in determining PBP localization...
  28. pmc Common beta-lactamases inhibit bacterial biofilm formation
    Claude V Gallant
    Department of Surgery, University of Toronto, Toronto, ON, Canada
    Mol Microbiol 58:1012-24. 2005
    ..Beta-lactamases likely affect PG remodelling, manifesting as perturbation of structures involved in bacterial adhesion that are required to initiate biofilm formation...
  29. ncbi Ampicillin/penicillin-binding protein interactions as a model drug-target system to optimize affinity pull-down and mass spectrometric strategies for target and pathway identification
    Moritz von Rechenberg
    Prolexys Pharmaceuticals Inc, Salt Lake City, UT 84116, USA
    Proteomics 5:1764-73. 2005
    ..The combination of drug affinity pull-down assays with in situ digestion and 2-D LC-MS/MS analysis is a useful tool in obtaining complex information about a primary drug target as well as its protein interactors...
  30. pmc Contribution of membrane-binding and enzymatic domains of penicillin binding protein 5 to maintenance of uniform cellular morphology of Escherichia coli
    David E Nelson
    Department of Microbiology and Immunology, University of North Dakota School of Medicine, Grand Forks, North Dakota 58202 9037, USA
    J Bacteriol 184:3630-9. 2002
    ....
  31. pmc FtsZ collaborates with penicillin binding proteins to generate bacterial cell shape in Escherichia coli
    Archana Varma
    Department of Microbiology and Immunology, University of North Dakota School of Medicine and Health Sciences, Grand Forks, ND 58202, USA
    J Bacteriol 186:6768-74. 2004
    ....
  32. pmc Overproduction of inactive variants of the murein synthase PBP1B causes lysis in Escherichia coli
    Ute Meisel
    Abteilung Biochemie, Max Planck Institut fur Entwicklungsbiologie, 72076 Tubingen, Germany
    J Bacteriol 185:5342-8. 2003
    ..A possible structural interaction between PBP1B and murein hydrolases in vivo by the formation of a multienzyme complex is discussed...
  33. ncbi Bacterial shape
    Kevin D Young
    Department of Microbiology and Immunology, University of North Dakota School of Medicine, Grand Forks 58202 9037, USA
    Mol Microbiol 49:571-80. 2003
    ....
  34. pmc Probing the catalytic activity of a cell division-specific transpeptidase in vivo with beta-lactams
    Christian Eberhardt
    Department of Microbiology, University of Iowa, Iowa City, Iowa 52242, USA
    J Bacteriol 185:3726-34. 2003
    ..We suggest that there might be allosteric activation of substrate binding...
  35. pmc Branching of Escherichia coli cells arises from multiple sites of inert peptidoglycan
    Miguel A de Pedro
    Centro de Biologia Molecular Severo Ochoa, Consejo Superior de Investigaciones Cientificas, Universidad Autonoma de Madrid, Campus de Cantoblanco, 28049 Madrid, Spain
    J Bacteriol 185:1147-52. 2003
    ..These observations suggest that in dacA mutants, branches and other morphological anomalies may arise from split polar caps or by de novo generation of new poles built around inert peptidoglycan patches in the side walls of the cell...
  36. pmc Biochemistry and comparative genomics of SxxK superfamily acyltransferases offer a clue to the mycobacterial paradox: presence of penicillin-susceptible target proteins versus lack of efficiency of penicillin as therapeutic agent
    Colette Goffin
    Center for Protein Engineering, Institut de Chimie, University of Liege, B 4000 Sart Tilman, Liege, Belgium
    Microbiol Mol Biol Rev 66:702-38, table of contents. 2002
    ..Shifting the wall peptidoglycan from the (4-->3) type to the (3-->3) type could help Mycobacterium tuberculosis and Mycobacterium leprae survive by making them penicillin resistant...
  37. ncbi Alterations of murein structure and of penicillin-binding proteins in minicells from Escherichia coli
    W Obermann
    Max Planck Institut fur Entwicklungsbiologie, Abteilung Biochemie, Tubingen, FRG
    Microbiology 140:79-87. 1994
    ..Whereas PBP4 and PBP6 were greatly diminished, PBP8 was significantly increased. We consider it unlikely that the sort of changes observed in murein structure reflect the fact that minicells are composed of two hemispherical polar caps...
  38. pmc Effects of multiple deletions of murein hydrolases on viability, septum cleavage, and sensitivity to large toxic molecules in Escherichia coli
    Christoph Heidrich
    Max Planck Institut fur Entwicklungsbiologie, Abteilung Biochemie, 72076 Tubingen, Germany
    J Bacteriol 184:6093-9. 2002
    ..coli...
  39. ncbi Prediction of signal sequence-dependent protein translocation in bacteria: assessment of the Escherichia coli minicell system
    D B Davies
    Department of Microbiology, Medical School, University of Bristol, United Kingdom
    Biochem Biophys Res Commun 150:371-5. 1988
    ..The results reported here suggest that PEA is a suitable probe for leader sequences in the minicell system. By using PEA we predict that PBP4 does not require a leader sequence for membrane insertion...
  40. pmc The balance between different peptidoglycan precursors determines whether Escherichia coli cells will elongate or divide
    K J Begg
    Department of Molecular Biology, University of Edinburgh, Scotland
    J Bacteriol 172:6697-703. 1990
    ..A change in the proportions of different kinds of peptide side chain in the peptidoglycan can therefore determine whether cells will divide...
  41. ncbi Cloning and characterization of mepA, the structural gene of the penicillin-insensitive murein endopeptidase from Escherichia coli
    W Keck
    Department of Biochemistry, University of Groningen, The Netherlands
    Mol Microbiol 4:209-19. 1990
    ..No significant sequence homology with the other (penicillin-sensitive) murein endopeptidase (dacB) or any other protein was found...
  42. ncbi Penicillin-binding protein 4 of Escherichia coli: molecular cloning of the dacB gene, controlled overexpression, and alterations in murein composition
    B Korat
    Max Planck Institut fur Entwicklungsbiologie, Tubingen, Germany
    Mol Microbiol 5:675-84. 1991
    ..Three positive clones were isolated, one of which carried the intact structural gene dacB that codes for PBP4, on a 1.9kb SmaI-EcoRI fragment, whereas the other two carried truncated forms of this gene...
  43. ncbi Penicillin-binding protein 4 of Escherichia coli shows a novel type of primary structure among penicillin-interacting proteins
    H Mottl
    Dept of Biochemistry, University of Groningen, The Netherlands
    FEMS Microbiol Lett 62:213-20. 1991
    The nucleotide sequence of a 1884 bp DNA fragment of E. coli, carrying the gene dacB, was determined...
  44. ncbi Purification of penicillin-binding protein 4 of Escherichia coli as a soluble protein by dye-affinity chromatography
    H Mottl
    University of Groningen, Department of Biochemistry, The Netherlands
    Eur J Biochem 200:767-73. 1991
    The dacB gene of Escherichia coli, coding for penicillin-binding protein 4 (PBP4) was cloned under the control of the phage lambda pR promoter and cro gene translation signals...
  45. pmc Deletion of an additional domain located between SXXK and SXN active-site fingerprints in penicillin-binding protein 4 from Escherichia coli
    H Mottl
    Department of Biochemistry, BIOSON Research Institute, University of Groningen, The Netherlands
    J Bacteriol 174:3261-9. 1992
    ..A clustered set of internal deletions was created in this region by exonuclease treatment of the dacB coding DNA, starting from two internal restriction sites...
  46. ncbi Enzymatic preparation of 1,6-anhydro-muropeptides by immobilized murein hydrolases from Escherichia coli fused to staphylococcal protein A
    H Engel
    Department of Biochemistry, BIOSON Research Institute, University of Groningen, The Netherlands
    Appl Microbiol Biotechnol 37:772-83. 1992
    ..The genes slt, dacB, and mepA, encoding the soluble lytic transglycosylase (Slt), the penicillin-sensitive DD-endopeptidase (PBP4), and ..
  47. pmc Separation of Escherichia coli penicillin-binding proteins into different membrane vesicles by agarose electrophoresis and sizing chromatography
    M J Leidenix
    Department of Microbiology and Immunology, University of North Dakota School of Medicine, Grand Forks 58202
    J Bacteriol 171:5680-6. 1989
    ..coli which is not observed when membrane vesicles are separated solely on the basis of density in sucrose gradients...
  48. pmc Mutational evidence for identity of penicillin-binding protein 5 in Escherichia coli with the major D-alanine carboxypeptidase IA activity
    M Matsuhashi
    J Bacteriol 137:644-7. 1979
    ..The results suggest that penicillin-binding protein 5 catalyzes the major D-alanine carboxypeptidase IA activity of the wild type and that the mutation results in a defect in the deacylation step catalyzed by this enzyme...
  49. pmc Isolation of a mutant of Escherichia coli lacking penicillin-sensitive D-alanine carboxypeptidase IA
    M Matsuhashi
    Proc Natl Acad Sci U S A 75:2631-5. 1978
    ..7 min) on the E. coli chromosome map. Double mutants (dacA dacB) that are deficient in both D-alanine carboxypeptidases IA and IB were obtained...
  50. pmc On the process of cellular division in Escherichia coli: a series of mutants of E. coli altered in the penicillin-binding proteins
    H Suzuki
    Proc Natl Acad Sci U S A 75:664-8. 1978
    ..With the series of mutants, the chromosomal locus of the gene for each PBC was mapped: ponA (PBC-1a) 73.5 min, ponB (PBC-1b) 3.3 min, rodA (PBC-2) 14.4 min, ftsI (PBC-3) 1.8 min, dacB (PBC-4) 68 min, and dacA (PBC-5/6) 13.7 min.
  51. pmc Mutants of Escherichia coli lacking in highly penicillin-sensitive D-alanine carboxypeptidase activity
    M Matsuhashi
    Proc Natl Acad Sci U S A 74:2976-9. 1977
    ..Genetic mapping studies show the mutation (dacB) to be located at 68 min on the E. coli chromosome map...
  52. ncbi Synthetic ColE1 Plasmids carrying genes for penicillin-binding proteins in Escherichia coli
    Y Takeda
    Plasmid 6:86-98. 1981
  53. ncbi Purification and light-scattering analysis of penicillin-binding protein 4 from Escherichia coli
    F Fusetti
    Department of Chemistry, University of Groningen, The Netherlands
    Microb Drug Resist 2:73-6. 1996
    ..Only by addition of EDTA, PEG 4000, and ammonium sulfate is the molecular mass about 110 kDa...
  54. pmc dacD, an Escherichia coli gene encoding a novel penicillin-binding protein (PBP6b) with DD-carboxypeptidase activity
    M R Baquero
    Unidad de Genetica Molecular, Hospital Ramon y Cajal, Madrid, Spain
    J Bacteriol 178:7106-11. 1996
    ..As with other E. coli DD-carboxypeptidases, PBP6b is not essential for cell growth. A quadruple dacA dacB dacC dacD mutant was constructed and shown to grow as well as its isogenic wild-type strain, indicating that the ..
  55. ncbi Multiple mechanisms of membrane anchoring of Escherichia coli penicillin-binding proteins
    J R Gittins
    Department of Biochemistry, University of Liverpool, UK
    FEMS Microbiol Rev 13:1-12. 1994
    ..It is possible that the PBPs and other inner membrane proteins form a peptidoglycan synthesizing complex to coordinate their activities...
  56. ncbi Purification, crystallisation and preliminary X-ray analysis of penicillin binding protein 4 from Escherichia coli, a protein related to class A beta-lactamases
    M M Thunnissen
    Department of Chemistry, University of Groningen, The Netherlands
    J Mol Biol 247:149-53. 1995
    ..They belong to space group C222 with cell dimensions a = 68.5 A, b = 100.5 A and c = 137.0 A, and diffract to at least 2.8 A resolution. There is one molecule with a molecule mass of 49,568 Da in the asymmetric unit...
  57. pmc Transcriptional control of dacB, which encodes a major sporulation-specific penicillin-binding protein
    E B Simpson
    Department of Biological Sciences, Southern Methodist University, Dallas, Texas 75275 0376
    J Bacteriol 176:7767-9. 1994
    ..sigma factor E (sigma E) of Bacillus subtilis is both necessary and sufficient for transcription of the dacB gene, which encodes penicillin-binding protein 5*...
  58. ncbi An investigation into the ability of C-terminal homologues of Escherichia coli low molecular mass penicillin-binding proteins 4, 5 and 6 to undergo membrane interaction
    F Harris
    Department of Applied Biology, University of Central Lancashire, Preston, United Kingdom
    Biochimie 79:171-4. 1997
    ..These results appear to support the present model for the membrane anchoring of PBP5 and PBP6. The PBP4 C-terminal homologue shows no evidence of haemolytic activity which could imply a different means of membrane association for PBP4...
  59. pmc On the process of cellular division in Escherichia coli: isolation and characterization of penicillin-binding proteins 1a, 1b, and 3
    T Tamura
    Proc Natl Acad Sci U S A 77:4499-503. 1980
    ..None of the three PBPs showed D-alanine carboxypeptidase activity with UDP-N-acetylmuramoyl-pentapeptide as substrate or endopeptidase activity with bis(disaccharide-peptide) as substrate...
  60. pmc Defective and plaque-forming lambda transducing bacteriophage carrying penicillin-binding protein-cell shape genes: genetic and physical mapping and identification of gene products from the lip-dacA-rodA-pbpA-leuS region of the Escherichia coli chromosome
    B G Spratt
    J Bacteriol 143:569-81. 1980
    ..Phage derivatives were constructed that allowed about 50-fold amplification of the levels of penicillin-binding proteins 2 and 5 in the cytoplasmic membrane...
  61. ncbi Mutants of Escherichia coli defective in penicillin-insensitive murein DD-endopeptidase
    K Iida
    Mol Gen Genet 189:215-21. 1983
    ..A double mutant (mepA mepB), and a triple mutant (mepA mepB dacB), deficient in both penicillin-insensitive and penicillin-sensitive endopeptidases, were constructed...
  62. pmc Loss of O-antigen increases cell shape abnormalities in penicillin-binding protein mutants of Escherichia coli
    Anindya S Ghosh
    Department of Biotechnology, Indian Institute of Technology, Kharagpur, WB, India
    FEMS Microbiol Lett 263:252-7. 2006
    ..Thus, the O-antigen contributes to cell shape in E. coli, perhaps by reducing the number of ectopic poles, which may be the proximal cause of shape abnormalities...