cyoB

Summary

Gene Symbol: cyoB
Description: cytochrome o ubiquinol oxidase subunit I
Alias: ECK0425, JW0421
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. ncbi Expression of cyoA and cyoB demonstrates that the CO-binding heme component of the Escherichia coli cytochrome o complex is in subunit I
    H Nakamura
    Department of Biology, Faculty of Science, University of Tokyo, Japan
    J Biol Chem 265:11193-7. 1990
  2. ncbi Transcriptional regulation of the cytochrome b562-o complex in Escherichia coli. Gene expression and molecular characterization of the promoter
    J Minagawa
    Department of Biology, Faculty of Science, University of Tokyo, Japan
    J Biol Chem 265:11198-203. 1990
  3. pmc Cloning of the cyo locus encoding the cytochrome o terminal oxidase complex of Escherichia coli
    D C Au
    J Bacteriol 169:3237-42. 1987
  4. ncbi Subunit II of the cytochrome bo3 ubiquinol oxidase from Escherichia coli is a lipoprotein
    J Ma
    School of Chemical Sciences, 600 South Mathews Street, University of Illinois, Urbana, Illinois 61801, USA
    Biochemistry 36:11298-303. 1997
  5. ncbi Identification of the residues involved in stabilization of the semiquinone radical in the high-affinity ubiquinone binding site in cytochrome bo(3) from Escherichia coli by site-directed mutagenesis and EPR spectroscopy
    Petra Hellwig
    Department of Biochemistry, University of Illinois, 600 South Mathews Street, Urbana, IL 61801, USA
    Biochemistry 41:10675-9. 2002
  6. ncbi [Extramedullary hematopoiesis with intraspinal extension in thalassemia]
    E Richter
    Abteilung Röntgendiagnostik der Universitätskinderklinik Hamburg
    Aktuelle Radiol 3:320-2. 1993
  7. ncbi Assignment and functional roles of the cyoABCDE gene products required for the Escherichia coli bo-type quinol oxidase
    H Nakamura
    Department of Biological Sciences, Graduate School of Science, The University of Tokyo, Hongo
    J Biochem 122:415-21. 1997
  8. pmc The fully oxidized form of the cytochrome bd quinol oxidase from E. coli does not participate in the catalytic cycle: direct evidence from rapid kinetics studies
    Ke Yang
    Department of Biochemistry, University of Illinois, Urbana, IL 61801, USA
    FEBS Lett 582:3705-9. 2008
  9. pmc Interactions of intermediate semiquinone with surrounding protein residues at the Q(H) site of wild-type and D75H mutant cytochrome bo3 from Escherichia coli
    Myat T Lin
    Center for Biophysics and Computational Biology, University of Illinois at Urbana Champaign, Urbana, Illinois 61801, USA
    Biochemistry 51:3827-38. 2012
  10. pmc Quantitative assessment of oxygen availability: perceived aerobiosis and its effect on flux distribution in the respiratory chain of Escherichia coli
    Svetlana Alexeeva
    Laboratory for Microbiology, Swammerdam Institute for Life Sciences, University of Amsterdam, Nieuwe Achtergracht 166, 1018 WV Amsterdam, The Netherlands
    J Bacteriol 184:1402-6. 2002

Scientific Experts

  • Hideki Kandori
  • Myles R Cheesman
  • P Hellwig
  • Lars J C Jeuken
  • Bradford E Peercy
  • Robert B Gennis
  • Lai Lai Yap
  • Myat T Lin
  • Sergei A Dikanov
  • Rimma I Samoilova
  • Kazuo Kobayashi
  • Tatsushi Mogi
  • Sophie A Weiss
  • J Abramson
  • Seiichi Tagawa
  • Tsuyoshi Egawa
  • Ke Yang
  • Sayan Gupta
  • Audrius Jasaitis
  • Gaye F White
  • Filippa Stenberg
  • Heather L Frericks
  • A Puustinen
  • Yushi Matsumoto
  • R B Gennis
  • Takeshi Uchida
  • Erin Ching
  • T Mogi
  • Farol Tomson
  • Svetlana Alexeeva
  • Patrick J O'Malley
  • Richard Hart
  • Sylvia K Choi
  • Amgalanbaatar Baldansuren
  • Kuppala V Narasimhulu
  • Hanlin Ouyang
  • Stephen D Evans
  • S Iwata
  • G Larsson
  • Richard J Bushby
  • M Wikstrom
  • Denis L Rousseau
  • Jonathan P Hosler
  • Syun Ru Yeh
  • Alexander A Konstantinov
  • Vitaliy B Borisov
  • Shyamalava Mazumdar
  • Y Anraku
  • Y Yamazaki
  • Andromachi Katsonouri
  • Mikael P Johansson
  • Marten H Vos
  • Daniel O Daley
  • Sarah Field
  • Andrew J Thomson
  • Sophie Marritt
  • Michael I Verkhovsky
  • Vasily S Oganesyan
  • H Nakamura
  • Gunnar von Heijne
  • Marten Wikstrom
  • Masasuke Yoshida
  • Hideto Miyoshi
  • Kimitoshi Sakamoto
  • Chad M Rienstra
  • Daisuke Fujita
  • Masatoshi Murai
  • Donghua H Zhou
  • Hiro Nakamura
  • M Kawasaki
  • S M Musser
  • J Ma
  • Teizo Kitagawa
  • Randy W Larsen
  • Louis A Silks
  • C P Tseng
  • Rodolfo A Martinez
  • M Joost Teixeira De Mattos
  • Robert J Donohoe
  • Clifford J Unkefer
  • Zizhong Li
  • James A Bailey
  • William H Woodruff
  • Klaas J Hellingwerf
  • R Brian Dyer
  • R P Gunsalus
  • P A Cotter
  • B Byrne
  • J E Morgan
  • M Svensson-Ek

Detail Information

Publications66

  1. ncbi Expression of cyoA and cyoB demonstrates that the CO-binding heme component of the Escherichia coli cytochrome o complex is in subunit I
    H Nakamura
    Department of Biology, Faculty of Science, University of Tokyo, Japan
    J Biol Chem 265:11193-7. 1990
    ..This paper describes results obtained by expressing independently cyoA and cyoB in the absence of the other subunits of the complex...
  2. ncbi Transcriptional regulation of the cytochrome b562-o complex in Escherichia coli. Gene expression and molecular characterization of the promoter
    J Minagawa
    Department of Biology, Faculty of Science, University of Tokyo, Japan
    J Biol Chem 265:11198-203. 1990
    ....
  3. pmc Cloning of the cyo locus encoding the cytochrome o terminal oxidase complex of Escherichia coli
    D C Au
    J Bacteriol 169:3237-42. 1987
    ..The cloned genes will be valuable for studying the structure, function, and regulation of the cytochrome o terminal oxidase complex...
  4. ncbi Subunit II of the cytochrome bo3 ubiquinol oxidase from Escherichia coli is a lipoprotein
    J Ma
    School of Chemical Sciences, 600 South Mathews Street, University of Illinois, Urbana, Illinois 61801, USA
    Biochemistry 36:11298-303. 1997
    ..Hence, this unusual processing of a cytoplasmic membrane protein, which is already anchored to the membrane by two transmembrane helices, is not essential for either assembly or function...
  5. ncbi Identification of the residues involved in stabilization of the semiquinone radical in the high-affinity ubiquinone binding site in cytochrome bo(3) from Escherichia coli by site-directed mutagenesis and EPR spectroscopy
    Petra Hellwig
    Department of Biochemistry, University of Illinois, 600 South Mathews Street, Urbana, IL 61801, USA
    Biochemistry 41:10675-9. 2002
    ..Details of the possible binding motif and mechanistic implications are discussed...
  6. ncbi [Extramedullary hematopoiesis with intraspinal extension in thalassemia]
    E Richter
    Abteilung Röntgendiagnostik der Universitätskinderklinik Hamburg
    Aktuelle Radiol 3:320-2. 1993
    ..A rapid improvement was observed after blood transfusions...
  7. ncbi Assignment and functional roles of the cyoABCDE gene products required for the Escherichia coli bo-type quinol oxidase
    H Nakamura
    Department of Biological Sciences, Graduate School of Science, The University of Tokyo, Hongo
    J Biochem 122:415-21. 1997
    ..Present findings suggest that subunits II, III, and IV of the oxidase complex are required for the assembly of the metal centers in subunit I...
  8. pmc The fully oxidized form of the cytochrome bd quinol oxidase from E. coli does not participate in the catalytic cycle: direct evidence from rapid kinetics studies
    Ke Yang
    Department of Biochemistry, University of Illinois, Urbana, IL 61801, USA
    FEBS Lett 582:3705-9. 2008
    ..The data support models of the catalytic cycle which do not include the fully oxidized form of the enzyme as an intermediate...
  9. pmc Interactions of intermediate semiquinone with surrounding protein residues at the Q(H) site of wild-type and D75H mutant cytochrome bo3 from Escherichia coli
    Myat T Lin
    Center for Biophysics and Computational Biology, University of Illinois at Urbana Champaign, Urbana, Illinois 61801, USA
    Biochemistry 51:3827-38. 2012
    ..In particular, we identify the factors responsible for the asymmetric distribution of the unpaired spin density and ponder the significance of this asymmetry to the quinone's electron transfer function...
  10. pmc Quantitative assessment of oxygen availability: perceived aerobiosis and its effect on flux distribution in the respiratory chain of Escherichia coli
    Svetlana Alexeeva
    Laboratory for Microbiology, Swammerdam Institute for Life Sciences, University of Amsterdam, Nieuwe Achtergracht 166, 1018 WV Amsterdam, The Netherlands
    J Bacteriol 184:1402-6. 2002
    ..coli (and specifically its respiratory activity) are affected by the actual oxygen availability per unit of biomass rather than by the residual dissolved oxygen concentration of the culture...
  11. ncbi Direct infrared detection of the covalently ring linked His-Tyr structure in the active site of the heme-copper oxidases
    Farol Tomson
    Department of Chemistry, University of Illinois at Urbana Champaign, Urbana, Illinois 61801, USA
    Biochemistry 41:14383-90. 2002
    ..This feature is readily detectable by infrared difference techniques, and offers a direct spectroscopic probe for potential radical production involving the H-Y structure in the O(2) reduction cycle of the oxidases...
  12. ncbi Effects of subunit I mutations on redox-linked conformational changes of the Escherichia coli bo-type ubiquinol oxidase revealed by Fourier-transform infrared spectroscopy
    Y Yamazaki
    Department of Biophysics, Graduate School of Science, Kyoto University, Sakyo ku, Kyoto, 606 8502, Japan
    J Biochem 126:194-9. 1999
    ..Localization and time-resolved analysis of the structural changes during dioxygen reduction will facilitate understanding of the molecular mechanism of redox-coupled proton pumping at the atomic level...
  13. pmc Kinetics of intramolecular electron transfer in cytochrome bo3 from Escherichia coli
    Erin Ching
    Department of Chemistry, University of Hawaii, Honolulu, Hawaii 96822, USA
    Biophys J 84:2728-33. 2003
    ....
  14. ncbi The nature of the exchange coupling between high-spin Fe(III) heme o3 and CuBII in Escherichia coli quinol oxidase, cytochrome bo3: MCD and EPR studies
    Myles R Cheesman
    School of Chemical Sciences and Pharmacy, Centre for Metalloprotein Spectroscopy and Biology CMSB, University of East Anglia, Norwich NR4 7TJ, U K
    J Am Chem Soc 126:4157-66. 2004
    ..These conclusions overturn a long-held view that such EPR signals in bovine cytochrome c oxidase arise from an S' = 2 ground state resulting from strong exchange coupling (J > 10(2) cm-1) within the active site...
  15. ncbi Direct evidence for the protonation of aspartate-75, proposed to be at a quinol binding site, upon reduction of cytochrome bo3 from Escherichia coli
    P Hellwig
    Department of Biochemistry, University of Illinois, 600 South Mathews Avenue, Urbana, Illinois 61801 3364, USA
    Biochemistry 40:1077-82. 2001
    ..It is suggested that upon reduction of the bound ubiquinone at the high affinity site, D75 takes up a proton, possibly sharing it with ubiquinol...
  16. ncbi Role of Tyr-288 at the dioxygen reduction site of cytochrome bo studied by stable isotope labeling and resonance raman spectroscopy
    Takeshi Uchida
    Okazaki Institute for Integrative Bioscience, National Institutes of Natural Sciences, Myodaiji, Okazaki, Aichi 444 8787, Japan
    J Biol Chem 279:53613-20. 2004
    ..Our results suggested that the substitution of ring hydrogens of Tyr-288 with deuteriums slows down the formation of the His-Tyr cross-link essential for dioxygen reduction at the binuclear center...
  17. ncbi Redox-induced protein structural changes in cytochrome bo revealed by Fourier transform infrared spectroscopy and [13C]Tyr labeling
    Hideki Kandori
    Department of Materials Science and Engineering, Nagoya Institute of Technology, Showa Ku, Nagoya 466 8555
    J Biol Chem 280:32821-6. 2005
    ....
  18. ncbi The structure of the ubiquinol oxidase from Escherichia coli and its ubiquinone binding site
    J Abramson
    Uppsala University, Department of Biochemistry, Biomedical Center Box 576, Uppsala S 75123, Sweden
    Nat Struct Biol 7:910-7. 2000
    ..In light of these findings we suggest specific roles for these polar residues in electron and proton transfer in ubiquinol oxidase...
  19. ncbi Mass spectrometric analysis of the ubiquinol-binding site in cytochrome bd from Escherichia coli
    Yushi Matsumoto
    Chemical Resources Laboratory, Tokyo Institute of Technology, Nagatsuta 4259, Midori ku, Yokohama 226 8503, Japan
    J Biol Chem 281:1905-12. 2006
    ....
  20. pmc Redox enzymes in tethered membranes
    Lars J C Jeuken
    Institute of Molecular Biophysics, School of Physics and Astronomy, Centre for Self Organising Molecular Systems, and Astbury Centre for Structural and Molecular Biology, University of Leeds, Leeds LS2 9JT, United Kingdom
    J Am Chem Soc 128:1711-6. 2006
    ..Enzyme coverages observed with TM-AFM and CV coincide (2-8.5 fmol.cm(-)(2)), indicating that most-if not all-cbo(3) on the surface is catalytically active and thus retains its integrity during immobilization...
  21. ncbi Characterization of the exchangeable protons in the immediate vicinity of the semiquinone radical at the QH site of the cytochrome bo3 from Escherichia coli
    Lai Lai Yap
    Department of Biochemistry, University of Illinois, Urbana, Illinois 61801, USA
    J Biol Chem 281:16879-87. 2006
    ..The results support a model that the semiquinone is bound to the protein in a very asymmetric manner by two strong hydrogen bonds from Asp-75 and Arg-71 to the O1 carbonyl, while the O4 carbonyl is not hydrogen-bonded to the protein...
  22. ncbi A kinetic model of oxygen regulation of cytochrome production in Escherichia coli
    Bradford E Peercy
    Computational and Applied Mathematics, Rice University, 6100 Main Str, MS 134, Houstin, TX 77005, USA
    J Theor Biol 242:547-63. 2006
    ..Toward this end we build and analyse a mathematical model that captures the role played by oxygen in the regulation of cytochrome production in E. coli...
  23. ncbi Magic-angle spinning solid-state NMR of a 144 kDa membrane protein complex: E. coli cytochrome bo3 oxidase
    Heather L Frericks
    Department of Chemistry, University of Illinois at Urbana Champaign, Urbana, IL 61801, USA
    J Biomol NMR 36:55-71. 2006
    ....
  24. ncbi Characterization of mutants that change the hydrogen bonding of the semiquinone radical at the QH site of the cytochrome bo3 from Escherichia coli
    Lai Lai Yap
    Department of Biochemistry, University of Illinois, Urbana, Illinois 61801, USA
    J Biol Chem 282:8777-85. 2007
    ..Either the redistribution of charge density in the semiquinone species, or the altered hydrogen bonding network is responsible for the loss of catalytic function...
  25. ncbi An EPR spin label study of the quinol oxidase, E. coli cytochrome bo3: a search for redox induced conformational changes
    Gaye F White
    School of Chemical Sciences and Pharmacy, University of East Anglia, Norwich, UK
    Biochemistry 46:2355-63. 2007
    ..Because kinetic studies of mutants show that the redox gating of protons occurs deep within the D channel close to the heme-copper site, the present study implies that no motion is transmitted to the ends of the helices...
  26. ncbi Assembly of the cytochrome bo3 complex
    Filippa Stenberg
    Center for Biomembrane Research, Department of Biochemistry and Biophysics, Stockholm University, Sweden
    J Mol Biol 371:765-73. 2007
    ..We also show that cofactor insertion catalyses assembly. These findings provide novel insights into the biogenesis of this model membrane protein complex...
  27. pmc Nanosecond electron tunneling between the hemes in cytochrome bo3
    Audrius Jasaitis
    Laboratoire d Optique et Biosciences, Centre National de la Recherche Scientifique, Ecole Polytechnique, 91128 Palaiseau, France
    Proc Natl Acad Sci U S A 104:20811-4. 2007
    ..The properties of eT between the closely lying heme groups in the heme-copper oxidases are discussed in terms of the reorganization energy for the process, and two methods for assessing the rate of electron tunneling are presented...
  28. ncbi Inhibition of bacterial oxidases by formamide and analogs
    Sayan Gupta
    Department of Chemical Sciences, Tata Institute of Fundamental Research, Homi Bhabha Road, Colaba, Mumbai 400 005, India
    Biol Chem 389:599-607. 2008
    ..This indicates that inhibition of enzyme activity by formamide possibly predominantly involves blocking of the water exit pathway in the oxidases...
  29. pmc Identification of the nitrogen donor hydrogen bonded with the semiquinone at the Q(H) site of the cytochrome bo3 from Escherichia coli
    Myat T Lin
    Department of Biophysics and Computational Biology, University of Illinois at Urbana Champaign, Urbana, Illinois 61801, USA
    J Am Chem Soc 130:15768-9. 2008
    ..Those are reflecting a distribution of the unpaired spin density over the protein in the SQ state of the quinone processing site...
  30. doi Intramolecular electron transfer processes in Cu(B)-deficient cytochrome bo studied by pulse radiolysis
    Kazuo Kobayashi
    Institute of Scientific and Industrial Research, Osaka University, Mihogaoka, Ibaraki, Osaka, Japan
    J Biochem 145:685-91. 2009
    ..And the absence of a putative OH(-) bound to Cu(B) seems not to affect the uptake of the first chemical proton via K-channel in the His333Ala mutant...
  31. pmc Impedance spectroscopy of bacterial membranes: coenzyme-Q diffusion in a finite diffusion layer
    Lars J C Jeuken
    Institute of Membrane and Systems Biology, School of Physics and Astronomy, and Centre for Self Organising Molecular Systems, University of Leeds, Leeds LS2 9JT, UK
    Anal Chem 80:9084-90. 2008
    ..It is hypothesized that this result represent perpendicular diffusion of quinone across the membrane, corresponding to a "flip" time between 0.05 and 1 s...
  32. ncbi Electron transfer processes in subunit I mutants of cytochrome bo quinol oxidase in Escherichia coli
    Kazuo Kobayashi
    Institute of Scientific and Industrial Research, Osaka University, Ibaraki, Osaka, Japan
    Biosci Biotechnol Biochem 73:1599-603. 2009
    ..Our results indicate that electromagnetic coupling of hemes is important for facilitated heme-heme electron transfer in cytochrome bo...
  33. pmc Communication between R481 and Cu(B) in cytochrome bo(3) ubiquinol oxidase from Escherichia coli
    Tsuyoshi Egawa
    Department of Physiology and Biophysics, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    Biochemistry 48:12113-24. 2009
    ..The implications of these data on the proton translocation mechanism are considered...
  34. pmc A study of cytochrome bo3 in a tethered bilayer lipid membrane
    Sophie A Weiss
    School of Physics and Astronomy, University of Leeds, Leeds, LS2 9JT, UK
    Biochim Biophys Acta 1797:1917-23. 2010
    ..This suggests that at Zn(II) concentration below 50µM the proton release of cbo(3) is inhibited, but not the proton uptake required to reduce oxygen to water...
  35. pmc The quinone-binding sites of the cytochrome bo3 ubiquinol oxidase from Escherichia coli
    Lai Lai Yap
    Department of Biochemistry, University of Illinois, 600 S Goodwin Avenue, Urbana, IL 61801, USA
    Biochim Biophys Acta 1797:1924-32. 2010
    ..It is suggested that the two quinone binding sites may be adjacent to each other or partially overlap...
  36. ncbi Cytochrome o (bo) is a proton pump in Paracoccus denitrificans and Escherichia coli
    A Puustinen
    Department of Medical Chemistry, University of Helsinki, Finland
    FEBS Lett 249:163-7. 1989
    ..coli from which cytochrome d was deleted. These observations show that the cytochrome o complex is a proton pump much like cytochrome aa3 to which it is structurally related...
  37. ncbi Determination of the ligands of the low spin heme of the cytochrome o ubiquinol oxidase complex using site-directed mutagenesis
    L J Lemieux
    School of Chemical Sciences, University of Illinois, Urbana 61801
    J Biol Chem 267:2105-13. 1992
    ....
  38. ncbi Contribution of the fnr and arcA gene products in coordinate regulation of cytochrome o and d oxidase (cyoABCDE and cydAB) genes in Escherichia coli
    P A Cotter
    Department of Microbiology and Molecular Genetics, University of California, Los Angeles 90024
    FEMS Microbiol Lett 70:31-6. 1992
    ..coli. Additionally, the expression of the fnr regulatory gene, and regulation of the anaerobic respiratory genes, narGHJI, dmsABC and frdABCD, was found to be independent of ArcA...
  39. ncbi Modified, large-scale purification of the cytochrome o complex (bo-type oxidase) of Escherichia coli yields a two heme/one copper terminal oxidase with high specific activity
    K C Minghetti
    Department of Biochemistry, University of Illinois, Urbana 61801
    Biochemistry 31:6917-24. 1992
    ..Partial amino acid sequence data confirm the identities of subunit I, II, and III from the SDS-PAGE analysis as the cyoB, cyoA, and cyoC gene products, respectively...
  40. ncbi The effect of iron limitation on expression of the aerobic and anaerobic electron transport pathway genes in Escherichia coli
    P A Cotter
    Department of Microbiology and Molecular Genetics, University of California, Los Angeles 90024
    FEMS Microbiol Lett 100:227-32. 1992
    ..The above findings suggest that in addition to Fur, some other cellular protein may bind iron for reporting and regulating iron-dependent cell functions...
  41. pmc E. coli map. Physical map locations of genes encoding components of the aerobic respiratory chain of Escherichia coli
    M W Calhoun
    Department of Biochemistry, University of Illinois, Urbana 61801
    J Bacteriol 173:1569-70. 1991
  42. ncbi Properties of the two terminal oxidases of Escherichia coli
    A Puustinen
    Department of Medical Chemistry, University of Helsinki, Finland
    Biochemistry 30:3936-42. 1991
    ..3-0.7. This was attributed to an "inside out" orientation of a significant fraction of the enzyme. Possible metabolic benefits of expressing both cytochromes bo and d in E. coli are discussed...
  43. ncbi The sequence of the cyo operon indicates substantial structural similarities between the cytochrome o ubiquinol oxidase of Escherichia coli and the aa3-type family of cytochrome c oxidases
    V Chepuri
    Department of Biochemistry, University of Illinois, Urbana 61801
    J Biol Chem 265:11185-92. 1990
    ..This operon is shown to encode five open reading frames, cyoABCDE. The gene products of three of these, cyoA, cyoB, and cyoC, are clearly related to subunits II, I, and III, respectively, of the eukaryotic and prokaryotic aa3-type ..
  44. ncbi The use of gene fusions to determine the topology of all of the subunits of the cytochrome o terminal oxidase complex of Escherichia coli
    V Chepuri
    Department of Biochemistry, University of Illinois, Urbana 61801
    J Biol Chem 265:12978-86. 1990
    ....
  45. ncbi Recent studies of the cytochrome o terminal oxidase complex of Escherichia coli
    V Chepuri
    Department of Biochemistry, University of Illinois, Urbana 61801
    Biochim Biophys Acta 1018:124-7. 1990
    ..Site-directed mutagenesis is being utilized to define which amino acids are ligands to the heme iron and copper prosthetic groups...
  46. pmc Requirement for terminal cytochromes in generation of the aerobic signal for the arc regulatory system in Escherichia coli: study utilizing deletions and lac fusions of cyo and cyd
    S Iuchi
    Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts 02115
    J Bacteriol 172:6020-5. 1990
    ....
  47. pmc Cytochrome o (cyoABCDE) and d (cydAB) oxidase gene expression in Escherichia coli is regulated by oxygen, pH, and the fnr gene product
    P A Cotter
    Department of Microbiology and Molecular Genetics, University of California, Los Angeles 90024 1489
    J Bacteriol 172:6333-8. 1990
    ..We also examined cyoABCDE and cydAB expression in response to growth on alternative carbon compounds and to changes in the culture medium pH and osmolarity...
  48. ncbi Genomic replacement in Escherichia coli K-12 using covalently closed circular plasmid DNA
    K L Oden
    Department of Biochemistry, University of Illinois, Urbana 61801
    Gene 96:29-36. 1990
    ..It is reported that such mutants may be constructed without linearizing plasmid DNA, as described previously...
  49. pmc Isolation and characterization of an Escherichia coli mutant lacking the cytochrome o terminal oxidase
    D C Au
    J Bacteriol 161:123-7. 1985
    ..Introduction of the cyd+ allele into the strain restores the respiration function of the strain, indicating that the cytochrome o branch of the respiratory chain is dispensable under normal laboratory growth conditions...
  50. ncbi Identification of a "peroxy" intermediate in cytochrome bo3 of Escherichia coli
    J E Morgan
    Helsinki Bioenergetics Group, Department of Medical Chemistry, University of Helsinki, Finland
    Biochemistry 34:15633-7. 1995
    ..This intermediate subsequently relaxes into the "ferryl" species which has been described previously. We conclude that the oxygen reaction is similar in the cytochrome c and quinol oxidases...
  51. ncbi Identification of the ubiquinol-binding site in the cytochrome bo3-ubiquinol oxidase of Escherichia coli
    R Welter
    Department of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater 74078
    J Biol Chem 269:28834-8. 1994
    ..This suggests that the ubiquinol-binding site of the cytochrome bo3 complex is located at least partially on subunit II...
  52. ncbi Substitution of asparagine for aspartate-135 in subunit I of the cytochrome bo ubiquinol oxidase of Escherichia coli eliminates proton-pumping activity
    J W Thomas
    School of Chemical Sciences, University of Illinois, Urbana 61801
    Biochemistry 32:10923-8. 1993
    ..However, the conservative mutation Asp135Glu is active in proton translocation. It is proposed that an acidic residue at position 135 in subunit I may be important to form a functional proton input channel of the proton pump...
  53. pmc Effect of microaerophilic cell growth conditions on expression of the aerobic (cyoABCDE and cydAB) and anaerobic (narGHJI, frdABCD, and dmsABC) respiratory pathway genes in Escherichia coli
    C P Tseng
    Department of Microbiology and Molecular Genetics, University of California, Los Angeles 90095, USA
    J Bacteriol 178:1094-8. 1996
    ..These two transcriptional regulators coordinate the hierarchial control of respiratory pathway gene expression in E. coli to ensure the optimal use of oxygen in the cell environment...
  54. ncbi Uncompetitive substrate inhibition and noncompetitive inhibition by 5-n-undecyl-6-hydroxy-4,7-dioxobenzothiazole (UHDBT) and 2-n-nonyl-4-hydroxyquinoline-N-oxide (NQNO) is observed for the cytochrome bo3 complex: implications for a Q(H2)-loop proton trans
    S M Musser
    Division of Chemistry and Chemical Engineering, Arthur Amos Noyes Laboratory of Chemical Physics, California Institute of Technology, Pasadena 91125, USA
    Biochemistry 36:894-902. 1997
    ....
  55. ncbi Identification of heme and copper ligands in subunit I of the cytochrome bo complex in Escherichia coli
    J Minagawa
    Department of Biology, Faculty of Science, University of Tokyo, Japan
    J Biol Chem 267:2096-104. 1992
    ..We present a helical wheel model of the redox center in subunit I, which consists of the membrane-spanning regions II, VI, VII, and X, and discuss the implications of the model...
  56. ncbi Substitutions of charged amino acid residues conserved in subunit I perturb the redox metal centers of the Escherichia coli bo-type ubiquinol oxidase
    M Kawasaki
    Department of Biological Sciences, Graduate School of Science, The University of Tokyo, Hongo
    J Biochem 122:422-9. 1997
    ..Based on these findings and recent crystallographic studies on cytochrome c oxidases, we discuss the possible roles of the conserved charged amino acid residues in subunit I of the heme-copper terminal oxidases...
  57. ncbi Purification, crystallization and preliminary crystallographic studies of an integral membrane protein, cytochrome bo3 ubiquinol oxidase from Escherichia coli
    J Abramson
    Uppsala University Department of Biochemistry, BMC BOX 576, S 75123 Uppsala, Sweden
    Acta Crystallogr D Biol Crystallogr 56:1076-8. 2000
    ....