cusC

Summary

Gene Symbol: cusC
Description: copper/silver efflux system, outer membrane component
Alias: ECK0564, JW0561, ibeB, silC, ylcB
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. ncbi Periplasmic metal-resistance protein CusF exhibits high affinity and specificity for both CuI and AgI
    Joshua T Kittleson
    Departments of Biochemistry and Molecular Biophysics, University of Arizona, Tucson, Arizona 85721, USA
    Biochemistry 45:11096-102. 2006
  2. pmc Unusual Cu(I)/Ag(I) coordination of Escherichia coli CusF as revealed by atomic resolution crystallography and X-ray absorption spectroscopy
    Isabell R Loftin
    Department of Biochemistry and Molecular Biophysics, University of Arizona, Tucson, Arizona 85721, USA
    Protein Sci 16:2287-93. 2007
  3. pmc Direct metal transfer between periplasmic proteins identifies a bacterial copper chaperone
    Ireena Bagai
    Department of Biochemistry and Molecular Biophysics, University of Arizona, Tucson, Arizona 85721, USA
    Biochemistry 47:11408-14. 2008
  4. ncbi Efflux-mediated heavy metal resistance in prokaryotes
    Dietrich H Nies
    Institute of Microbiology, Molecular Microbiology, Martin Luther University Halle Wittenberg, Kurt Mothes Strasse 3, 06099 Halle Saale, Germany
    FEMS Microbiol Rev 27:313-39. 2003
  5. ncbi Escherichia coli mechanisms of copper homeostasis in a changing environment
    Christopher Rensing
    Department of Soil, Water, and Environmental Science, University of Arizona, Shantz Bld 38, Rm 429, Tucson, AZ 85721, USA
    FEMS Microbiol Rev 27:197-213. 2003
  6. pmc Cu(I) recognition via cation-pi and methionine interactions in CusF
    Yi Xue
    Department of Chemistry, Northwestern University, 2145 Sheridan Road, Evanston, Illinois 60208, USA
    Nat Chem Biol 4:107-9. 2008
  7. ncbi Crystal structure of bacterial multidrug efflux transporter AcrB
    Satoshi Murakami
    Department of Cell Membrane Biology, Institute of Scientific and Industrial Research, Osaka University, Ibaraki, Osaka 567 0047, Japan
    Nature 419:587-93. 2002
  8. pmc Molecular analysis of the copper-transporting efflux system CusCFBA of Escherichia coli
    Sylvia Franke
    Molekulare Mikrobiologie, Institut fur Mikrobiologie, Martin Luther Universitat Halle Wittenberg, 06099 Halle, Germany
    J Bacteriol 185:3804-12. 2003
  9. pmc Crystal structures of CusC review conformational changes accompanying folding and transmembrane channel formation
    Hsiang Ting Lei
    Department of Chemistry, Department of Physics and Astronomy, Iowa State University, Ames, IA 50011, USA
    J Mol Biol 426:403-11. 2014
  10. pmc Crystal structure of Escherichia coli CusC, the outer membrane component of a heavy metal efflux pump
    Rithika Kulathila
    University of Massachusetts Medical School, Program in Molecular Medicine, Worcester, Massachusetts, United States of America
    PLoS ONE 6:e15610. 2011

Research Grants

Scientific Experts

  • Simon Silver
  • Sheng He Huang
  • David Stroebel
  • Christopher Rensing
  • D H Nies
  • A Gupta
  • Megan M McEvoy
  • Isabell R Loftin
  • Ireena Bagai
  • Sylvia Franke
  • Ninian J Blackburn
  • Hsiang Ting Lei
  • Akihito Yamaguchi
  • Rithika Kulathila
  • Katherine J Franz
  • Yi Xue
  • Chun Nam Lok
  • Lee Macomber
  • G Grass
  • F W Outten
  • Joshua T Kittleson
  • Satoshi Yamada
  • Kaneyoshi Yamamoto
  • Lucy J Lee
  • Hidetada Hirakawa
  • Corinna Bleuel
  • Yisheng Kang
  • Andrei V Astashkin
  • Gregor Grass
  • Satoshi Murakami
  • Nicholas R Bishop
  • Chih Chia Su
  • Jani Reddy Bolla
  • Edward W Yu
  • S Franke
  • Bert van den Berg
  • Mridhu Indic
  • Ragini Kulathila
  • T V O'Halloran
  • G P Munson
  • Jay P Stasser
  • Benjamin M Staehlin
  • Chi Ming Che
  • Jen Fu Chiu
  • Chi Ming Ho
  • Anna V Davis
  • Gurusamy Balakrishnan
  • Thomas G Spiro
  • Pamela Focia
  • THOMAS V O'HALLORAN
  • Paul Kwong Hang Tam
  • Rong Chen
  • James E Penner-Hahn
  • Wenbo Liu
  • James A Imlay
  • Kevin P Engelhardt
  • Eri Maeda
  • Kyoko Inubushi
  • Hiroshi Takagi
  • Kunihiko Nishino
  • Shigeru Nakamori
  • Andrew C Hausrath
  • Naoki Awano
  • Frederick R Blattner
  • Patricia J Kiley
  • Takeshi Masaki
  • Nadine Taudte
  • Akira Ishihama
  • Robert K Poole
  • Gerd J Krauss
  • Judith Scherer
  • Yoshihiko Inazumi
  • Annie Heroux
  • William R Montfort
  • K Derek Weber
  • Takahiro Hirata
  • Cornelia Grosse
  • Sue A Roberts
  • Dirk Wesenberg
  • Jason A Barrett
  • F Ann Walker
  • Yu Qiu
  • Andrzej Weichsel
  • Arnold M Raitsimring
  • X Z Li
  • Eiki Yamashita
  • Ryosuke Nakashima
  • J A Hale
  • D L Huffman
  • D L Lam

Detail Information

Publications31

  1. ncbi Periplasmic metal-resistance protein CusF exhibits high affinity and specificity for both CuI and AgI
    Joshua T Kittleson
    Departments of Biochemistry and Molecular Biophysics, University of Arizona, Tucson, Arizona 85721, USA
    Biochemistry 45:11096-102. 2006
    ..The NMR spectra of CusF in the presence of Cu(II) do not indicate specific binding, which is in agreement with the ITC data. We conclude that Cu(I) and Ag(I) are the likely physiological substrates...
  2. pmc Unusual Cu(I)/Ag(I) coordination of Escherichia coli CusF as revealed by atomic resolution crystallography and X-ray absorption spectroscopy
    Isabell R Loftin
    Department of Biochemistry and Molecular Biophysics, University of Arizona, Tucson, Arizona 85721, USA
    Protein Sci 16:2287-93. 2007
    ..The arrangement of ligands effectively sequesters the metal from its periplasmic environment and thus may play a role in protecting the cell from the toxic ion...
  3. pmc Direct metal transfer between periplasmic proteins identifies a bacterial copper chaperone
    Ireena Bagai
    Department of Biochemistry and Molecular Biophysics, University of Arizona, Tucson, Arizona 85721, USA
    Biochemistry 47:11408-14. 2008
    ..These experiments establish a metallochaperone activity for CusF in the periplasm of gram-negative bacteria, serving to protect the periplasm from metal-mediated damage...
  4. ncbi Efflux-mediated heavy metal resistance in prokaryotes
    Dietrich H Nies
    Institute of Microbiology, Molecular Microbiology, Martin Luther University Halle Wittenberg, Kurt Mothes Strasse 3, 06099 Halle Saale, Germany
    FEMS Microbiol Rev 27:313-39. 2003
    ..Possession of the latter systems makes a bacterium heavy metal resistant...
  5. ncbi Escherichia coli mechanisms of copper homeostasis in a changing environment
    Christopher Rensing
    Department of Soil, Water, and Environmental Science, University of Arizona, Shantz Bld 38, Rm 429, Tucson, AZ 85721, USA
    FEMS Microbiol Rev 27:197-213. 2003
    ..Pathways of copper uptake and intracellular copper handling are still not identified in E. coli...
  6. pmc Cu(I) recognition via cation-pi and methionine interactions in CusF
    Yi Xue
    Department of Chemistry, Northwestern University, 2145 Sheridan Road, Evanston, Illinois 60208, USA
    Nat Chem Biol 4:107-9. 2008
    ..This novel active site chemistry affords mechanisms for control of adventitious metal redox and substitution chemistry...
  7. ncbi Crystal structure of bacterial multidrug efflux transporter AcrB
    Satoshi Murakami
    Department of Cell Membrane Biology, Institute of Scientific and Industrial Research, Osaka University, Ibaraki, Osaka 567 0047, Japan
    Nature 419:587-93. 2002
    ....
  8. pmc Molecular analysis of the copper-transporting efflux system CusCFBA of Escherichia coli
    Sylvia Franke
    Molekulare Mikrobiologie, Institut fur Mikrobiologie, Martin Luther Universitat Halle Wittenberg, 06099 Halle, Germany
    J Bacteriol 185:3804-12. 2003
    ..CusA and CusB were essential for copper resistance, and CusC and CusF were required for full resistance...
  9. pmc Crystal structures of CusC review conformational changes accompanying folding and transmembrane channel formation
    Hsiang Ting Lei
    Department of Chemistry, Department of Physics and Astronomy, Iowa State University, Ames, IA 50011, USA
    J Mol Biol 426:403-11. 2014
    ..To remove these toxic ions, the CusC outer membrane channel must form a β-barrel structural domain, which creates a pore and spans the entire outer ..
  10. pmc Crystal structure of Escherichia coli CusC, the outer membrane component of a heavy metal efflux pump
    Rithika Kulathila
    University of Massachusetts Medical School, Program in Molecular Medicine, Worcester, Massachusetts, United States of America
    PLoS ONE 6:e15610. 2011
    ..The cusCBFA operon of E. coli encodes a four-component efflux pump dedicated to the extrusion of Cu(I) and Ag(I) ions...
  11. pmc Genome-wide expression analysis indicates that FNR of Escherichia coli K-12 regulates a large number of genes of unknown function
    Yisheng Kang
    Department of Genetics, 425 Henry Mall, University of Wisconsin, Madison, WI 53706, USA
    J Bacteriol 187:1135-60. 2005
    ..We suggest that E. coli MG1655 has a larger metabolic potential under anaerobic conditions than has been previously recognized...
  12. doi Copper shares a piece of the pi
    Katherine J Franz
    Nat Chem Biol 4:85-6. 2008
  13. doi Proteomic identification of the Cus system as a major determinant of constitutive Escherichia coli silver resistance of chromosomal origin
    Chun Nam Lok
    The Open Laboratory of Chemical Biology of the Institute of Molecular Technology for Drug Discovery and Synthesis, Department of Chemistry, Department of Anatomy, and Department of Surgery, The University of Hong Kong, Pokfulam, Hong Kong, China
    J Proteome Res 7:2351-6. 2008
    ..These results suggest that the chromosomally encoded Cus system, which naturally controls the periplasmic copper concentrations, is selectable to confer a constitutive silver resistance phenotype...
  14. ncbi Substrate-linked conformational change in the periplasmic component of a Cu(I)/Ag(I) efflux system
    Ireena Bagai
    Department of Biochemistry, University of Arizona, Tucson, Arizona 85721, USA
    J Biol Chem 282:35695-702. 2007
    ....
  15. ncbi Oligomeric behavior of the RND transporters CusA and AcrB in micellar solution of detergent
    David Stroebel
    Laboratoire des Proteines Membranaires, Institut de Biologie Structurale, Jean Pierre EBEL, UMR 5075 CNRS CEA UJF, 41, rue Jules Horowitz, 38027 Grenoble Cedex, France
    Biochim Biophys Acta 1768:1567-73. 2007
    ..This pseudo-heterogeneity does not hamper the crystallization of AcrB as a homotrimer...
  16. pmc Intracellular copper does not catalyze the formation of oxidative DNA damage in Escherichia coli
    Lee Macomber
    Department of Microbiology, University of Illinois, Urbana, IL 61801, USA
    J Bacteriol 189:1616-26. 2007
    ..These observations do not explain how copper suppresses iron-mediated damage. However, it is clear that copper does not catalyze significant oxidative DNA damage in vivo; therefore, copper toxicity must occur by a different mechanism...
  17. ncbi Characterization of the copper(II) binding site in the pink copper binding protein CusF by electron paramagnetic resonance spectroscopy
    Andrei V Astashkin
    Department of Chemistry, University of Arizona, Tucson, AZ 85721, USA
    J Biol Inorg Chem 10:221-30. 2005
    ..Apart from the two nitrogen ligands, it has been established that there are two nearby hydroxyl protons, although whether these belong to a single equatorial water ligand or two equatorial hydroxide ligands is not known...
  18. pmc Effect of drug transporter genes on cysteine export and overproduction in Escherichia coli
    Satoshi Yamada
    Department of Bioscience, Fukui Prefectural University, Fukui, Japan
    Appl Environ Microbiol 72:4735-42. 2006
    ..These results indicate that the multidrug transporter Bcr in the major facilitator family is involved in L-cysteine export and overproduction in genetically engineered E. coli cells...
  19. pmc TolC is involved in enterobactin efflux across the outer membrane of Escherichia coli
    Corinna Bleuel
    Institut fur Mikrobiologie, Martin Luther Universität Halle, Halle, Germany, Kurt Mothes Str 3, 06120 Halle, Germany
    J Bacteriol 187:6701-7. 2005
    ..However, iron starvation led to increased expression of the RND gene mdtF and a decrease in acrD...
  20. ncbi A novel copper-binding fold for the periplasmic copper resistance protein CusF
    Isabell R Loftin
    Department of Biochemistry and Molecular Biophysics, University of Arizona, Tucson, Arizona 85721, USA
    Biochemistry 44:10533-40. 2005
    ..The unique structure and metal binding site of CusF are distinct from those of previously characterized copper-binding proteins...
  21. pmc Silver-resistant mutants of Escherichia coli display active efflux of Ag+ and are deficient in porins
    X Z Li
    Department of Microbiology, University of Saskatchewan, Saskatoon, Canada
    J Bacteriol 179:6127-32. 1997
    ..The results suggest that active efflux, presumably coded by a chromosomal gene(s), may play a major role in silver resistance, which is likely to be enhanced synergistically by decreases in OM permeability...
  22. ncbi Indole induces the expression of multidrug exporter genes in Escherichia coli
    Hidetada Hirakawa
    Department of Cell Membrane Biology, Institute of Scientific and Industrial Research, Osaka University, 8 1 Mihogaoka, Ibaraki, Osaka 567 0047, Japan
    Mol Microbiol 55:1113-26. 2005
    ..BaeR and CpxR directly bound to different sequences of the acrD and mdtA promoter regions. These observations indicate that BaeR is a primary regulator, and CpxR enhances the effect of BaeR...
  23. pmc Genome-wide transcriptional response of chemostat-cultured Escherichia coli to zinc
    Lucy J Lee
    Department of Molecular Biology and Biotechnology, The University of Sheffield, Firth Court, Western Bank, Sheffield S10 2TN, United Kingdom
    J Bacteriol 187:1124-34. 2005
    ....
  24. ncbi Bacterial silver resistance: molecular biology and uses and misuses of silver compounds
    Simon Silver
    Department of Microbiology and Immunology, M C 790, University of Illinois, 835 South Wolcott Avenue, Chicago, IL 60612 7344, USA
    FEMS Microbiol Rev 27:341-53. 2003
    ..Ag is generally without adverse effects for humans, and argyria (irreversible discoloration of the skin resulting from subepithelial silver deposits) is rare and mostly of cosmetic concern...
  25. ncbi Diversity of silver resistance genes in IncH incompatibility group plasmids
    A Gupta
    Department of Microbiology and Immunology, University of Illinois, M C 790, 835 S Wolcott Ave, Room E 703, Chicago, IL 60612 7344, USA
    Microbiology 147:3393-402. 2001
    ..The inducibility of silC, silR and silE gene expression after Ag(+) exposure was studied by reverse transcriptase (RT)-PCR...
  26. ncbi The product of the ybdE gene of the Escherichia coli chromosome is involved in detoxification of silver ions
    S Franke
    Institut für Mikrobiologie der Martin Luther Universität Halle Wittenberg, Kurt Mothes Str 3, 06099 Halle, Germany
    Microbiology 147:965-72. 2001
    ..Thus, in E. coli, the YlcBCD--YbdE system may be involved in silver- but not in copper resistance, and CopA may be involved in copper- but not in silver resistance...
  27. pmc Genes involved in copper homeostasis in Escherichia coli
    G Grass
    Department of Soil, Water, and Environmental Science, University of Arizona, Tucson, Arizona 85721, USA
    J Bacteriol 183:2145-7. 2001
    ..In this report, data are presented that support a hypothesis that the putative multicopper oxidase CueO and the transenvelope transporter CusCFBA are involved in copper tolerance in E. coli...
  28. pmc Identification of a copper-responsive two-component system on the chromosome of Escherichia coli K-12
    G P Munson
    Department of Biochemistry, Molecular Biology, and Cell Biology, Northwestern University, Evanston, Illinois 60208 3113, USA
    J Bacteriol 182:5864-71. 2000
    ..at least one chromosomal gene, designated cusC (ylcB), which is allelic to the recently identified virulence gene ibeB in E. coli K1...
  29. pmc Identification and characterization of an Escherichia coli invasion gene locus, ibeB, required for penetration of brain microvascular endothelial cells
    S H Huang
    Division of Infectious Diseases, Childrens Hospital Los Angeles, Los Angeles, California 90027, USA
    Infect Immun 67:2103-9. 1999
    ..coli K1 strain RS218 (O18:K1:H7). Here we report the identification of another chromosomal locus, ibeB, which allows RS218 to invade brain microvascular endothelial cells (BMEC)...
  30. ncbi The independent cue and cus systems confer copper tolerance during aerobic and anaerobic growth in Escherichia coli
    F W Outten
    Department of Biochemistry, Northwestern University, Evanston, Illinois 60208, USA
    J Biol Chem 276:30670-7. 2001
    ..These results underscore the increased toxicity of copper under anaerobic conditions and the complex adaptation of copper export in E. coli...
  31. ncbi Transcriptional response of Escherichia coli to external copper
    Kaneyoshi Yamamoto
    Department of Agricultural Chemistry, Kinki University, Nakamachi 3327 204, Nara 631 8505, Japan
    Mol Microbiol 56:215-27. 2005
    ..three hitherto-identified copper-responsive genes, copA (copper efflux transporter), cueO (multicopper oxidase) and cusC (tripartite copper pump component) became maximum at 5 min after addition of copper sulphate, and thereafter ..

Research Grants1

  1. ESCHERICHIA COLI INVASION OF BRAIN ENDOTHELIAL CELLS
    Sheng He Huang; Fiscal Year: 2007
    ..To further characterize the role of IbeABP in E. coil K1 invasion of human BMEC. (3). To determine how E. coil K1 invasion gene expression is regulated by the IbeR and environmental signals (glucose and oxygen tension). ..