cusA

Summary

Gene Symbol: cusA
Description: copper/silver efflux system, membrane component
Alias: ECK0567, JW0564, silA, ybdE
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. ncbi The product of the ybdE gene of the Escherichia coli chromosome is involved in detoxification of silver ions
    S Franke
    Institut für Mikrobiologie der Martin Luther Universität Halle Wittenberg, Kurt Mothes Str 3, 06099 Halle, Germany
    Microbiology 147:965-72. 2001
  2. ncbi Oligomeric behavior of the RND transporters CusA and AcrB in micellar solution of detergent
    David Stroebel
    Laboratoire des Proteines Membranaires, Institut de Biologie Structurale, Jean Pierre EBEL, UMR 5075 CNRS CEA UJF, 41, rue Jules Horowitz, 38027 Grenoble Cedex, France
    Biochim Biophys Acta 1768:1567-73. 2007
  3. ncbi Crystal structure of bacterial multidrug efflux transporter AcrB
    Satoshi Murakami
    Department of Cell Membrane Biology, Institute of Scientific and Industrial Research, Osaka University, Ibaraki, Osaka 567 0047, Japan
    Nature 419:587-93. 2002
  4. pmc Molecular analysis of the copper-transporting efflux system CusCFBA of Escherichia coli
    Sylvia Franke
    Molekulare Mikrobiologie, Institut fur Mikrobiologie, Martin Luther Universitat Halle Wittenberg, 06099 Halle, Germany
    J Bacteriol 185:3804-12. 2003
  5. pmc Unusual Cu(I)/Ag(I) coordination of Escherichia coli CusF as revealed by atomic resolution crystallography and X-ray absorption spectroscopy
    Isabell R Loftin
    Department of Biochemistry and Molecular Biophysics, University of Arizona, Tucson, Arizona 85721, USA
    Protein Sci 16:2287-93. 2007
  6. ncbi Efflux-mediated heavy metal resistance in prokaryotes
    Dietrich H Nies
    Institute of Microbiology, Molecular Microbiology, Martin Luther University Halle Wittenberg, Kurt Mothes Strasse 3, 06099 Halle Saale, Germany
    FEMS Microbiol Rev 27:313-39. 2003
  7. pmc Cu(I) recognition via cation-pi and methionine interactions in CusF
    Yi Xue
    Department of Chemistry, Northwestern University, 2145 Sheridan Road, Evanston, Illinois 60208, USA
    Nat Chem Biol 4:107-9. 2008
  8. pmc Crystal structures of the CusA efflux pump suggest methionine-mediated metal transport
    Feng Long
    Molecular, Cellular and Developmental Biology Interdepartmental Graduate Program, Iowa State University, Iowa 50011, USA
    Nature 467:484-8. 2010
  9. ncbi Periplasmic metal-resistance protein CusF exhibits high affinity and specificity for both CuI and AgI
    Joshua T Kittleson
    Departments of Biochemistry and Molecular Biophysics, University of Arizona, Tucson, Arizona 85721, USA
    Biochemistry 45:11096-102. 2006
  10. ncbi A novel copper-binding fold for the periplasmic copper resistance protein CusF
    Isabell R Loftin
    Department of Biochemistry and Molecular Biophysics, University of Arizona, Tucson, Arizona 85721, USA
    Biochemistry 44:10533-40. 2005

Scientific Experts

  • Simon Silver
  • David Stroebel
  • Christopher Rensing
  • D H Nies
  • A Gupta
  • Megan M McEvoy
  • Isabell R Loftin
  • Lee Macomber
  • Ireena Bagai
  • Sylvia Franke
  • Ninian J Blackburn
  • Akihito Yamaguchi
  • Feng Long
  • Lucas B Pontel
  • Aurelien Deniaud
  • James A Imlay
  • Katherine J Franz
  • Chun Nam Lok
  • Yi Xue
  • G Grass
  • F W Outten
  • Satoshi Yamada
  • Joshua T Kittleson
  • Hidetada Hirakawa
  • Andrei V Astashkin
  • Lucy J Lee
  • Corinna Bleuel
  • Gregor Grass
  • Satoshi Murakami
  • S Franke
  • T V O'Halloran
  • Robert L Jernigan
  • Scott E Boyken
  • Michael T Zimmermann
  • Chih Chia Su
  • G P Munson
  • Kanagalaghatta R Rajashankar
  • Edward W Yu
  • Eva Pebay-Peyroula
  • Aurélie Goulielmakis
  • Jacques Coves
  • Fernando C Soncini
  • Pamela Focia
  • Chi Ming Che
  • Benjamin M Staehlin
  • Rong Chen
  • Gurusamy Balakrishnan
  • Chi Ming Ho
  • Thomas G Spiro
  • Jen Fu Chiu
  • Anna V Davis
  • THOMAS V O'HALLORAN
  • James E Penner-Hahn
  • Paul Kwong Hang Tam
  • Jay P Stasser
  • Wenbo Liu
  • Shigeru Nakamori
  • Eri Maeda
  • Kyoko Inubushi
  • Andrew C Hausrath
  • Naoki Awano
  • Hiroshi Takagi
  • Kevin P Engelhardt
  • Kunihiko Nishino
  • Annie Heroux
  • F Ann Walker
  • Cornelia Grosse
  • Andrzej Weichsel
  • Dirk Wesenberg
  • Takeshi Masaki
  • Robert K Poole
  • Arnold M Raitsimring
  • Gerd J Krauss
  • Takahiro Hirata
  • Sue A Roberts
  • Nadine Taudte
  • William R Montfort
  • Judith Scherer
  • Jason A Barrett
  • Yoshihiko Inazumi
  • X Z Li
  • Ryosuke Nakashima
  • Eiki Yamashita
  • J A Hale
  • D L Huffman
  • D L Lam
  • K E Williams
  • H Nikaido
  • J Pi
  • P J Wookey

Detail Information

Publications31

  1. ncbi The product of the ybdE gene of the Escherichia coli chromosome is involved in detoxification of silver ions
    S Franke
    Institut für Mikrobiologie der Martin Luther Universität Halle Wittenberg, Kurt Mothes Str 3, 06099 Halle, Germany
    Microbiology 147:965-72. 2001
    Transcription of the ybcZ--ylcA ylcBCD--ybdE region of the Escherichia coli K38 chromosome was analysed by Northern RNA--DNA hybridization, RT-PCR and primer extension...
  2. ncbi Oligomeric behavior of the RND transporters CusA and AcrB in micellar solution of detergent
    David Stroebel
    Laboratoire des Proteines Membranaires, Institut de Biologie Structurale, Jean Pierre EBEL, UMR 5075 CNRS CEA UJF, 41, rue Jules Horowitz, 38027 Grenoble Cedex, France
    Biochim Biophys Acta 1768:1567-73. 2007
    We have used analytical ultracentrifugation to explore the oligomeric states of AcrB and CusA in micellar solution of detergent...
  3. ncbi Crystal structure of bacterial multidrug efflux transporter AcrB
    Satoshi Murakami
    Department of Cell Membrane Biology, Institute of Scientific and Industrial Research, Osaka University, Ibaraki, Osaka 567 0047, Japan
    Nature 419:587-93. 2002
    ....
  4. pmc Molecular analysis of the copper-transporting efflux system CusCFBA of Escherichia coli
    Sylvia Franke
    Molekulare Mikrobiologie, Institut fur Mikrobiologie, Martin Luther Universitat Halle Wittenberg, 06099 Halle, Germany
    J Bacteriol 185:3804-12. 2003
    ..b>CusA and CusB were essential for copper resistance, and CusC and CusF were required for full resistance...
  5. pmc Unusual Cu(I)/Ag(I) coordination of Escherichia coli CusF as revealed by atomic resolution crystallography and X-ray absorption spectroscopy
    Isabell R Loftin
    Department of Biochemistry and Molecular Biophysics, University of Arizona, Tucson, Arizona 85721, USA
    Protein Sci 16:2287-93. 2007
    ..The arrangement of ligands effectively sequesters the metal from its periplasmic environment and thus may play a role in protecting the cell from the toxic ion...
  6. ncbi Efflux-mediated heavy metal resistance in prokaryotes
    Dietrich H Nies
    Institute of Microbiology, Molecular Microbiology, Martin Luther University Halle Wittenberg, Kurt Mothes Strasse 3, 06099 Halle Saale, Germany
    FEMS Microbiol Rev 27:313-39. 2003
    ..Possession of the latter systems makes a bacterium heavy metal resistant...
  7. pmc Cu(I) recognition via cation-pi and methionine interactions in CusF
    Yi Xue
    Department of Chemistry, Northwestern University, 2145 Sheridan Road, Evanston, Illinois 60208, USA
    Nat Chem Biol 4:107-9. 2008
    ..This novel active site chemistry affords mechanisms for control of adventitious metal redox and substitution chemistry...
  8. pmc Crystal structures of the CusA efflux pump suggest methionine-mediated metal transport
    Feng Long
    Molecular, Cellular and Developmental Biology Interdepartmental Graduate Program, Iowa State University, Iowa 50011, USA
    Nature 467:484-8. 2010
    ..Here we describe the crystal structures of the inner-membrane transporter CusA in the absence and presence of bound Cu(I) or Ag(I)...
  9. ncbi Periplasmic metal-resistance protein CusF exhibits high affinity and specificity for both CuI and AgI
    Joshua T Kittleson
    Departments of Biochemistry and Molecular Biophysics, University of Arizona, Tucson, Arizona 85721, USA
    Biochemistry 45:11096-102. 2006
    ..The NMR spectra of CusF in the presence of Cu(II) do not indicate specific binding, which is in agreement with the ITC data. We conclude that Cu(I) and Ag(I) are the likely physiological substrates...
  10. ncbi A novel copper-binding fold for the periplasmic copper resistance protein CusF
    Isabell R Loftin
    Department of Biochemistry and Molecular Biophysics, University of Arizona, Tucson, Arizona 85721, USA
    Biochemistry 44:10533-40. 2005
    ..The unique structure and metal binding site of CusF are distinct from those of previously characterized copper-binding proteins...
  11. pmc Intracellular copper does not catalyze the formation of oxidative DNA damage in Escherichia coli
    Lee Macomber
    Department of Microbiology, University of Illinois, Urbana, IL 61801, USA
    J Bacteriol 189:1616-26. 2007
    ..These observations do not explain how copper suppresses iron-mediated damage. However, it is clear that copper does not catalyze significant oxidative DNA damage in vivo; therefore, copper toxicity must occur by a different mechanism...
  12. ncbi Substrate-linked conformational change in the periplasmic component of a Cu(I)/Ag(I) efflux system
    Ireena Bagai
    Department of Biochemistry, University of Arizona, Tucson, Arizona 85721, USA
    J Biol Chem 282:35695-702. 2007
    ....
  13. doi Copper shares a piece of the pi
    Katherine J Franz
    Nat Chem Biol 4:85-6. 2008
  14. doi Proteomic identification of the Cus system as a major determinant of constitutive Escherichia coli silver resistance of chromosomal origin
    Chun Nam Lok
    The Open Laboratory of Chemical Biology of the Institute of Molecular Technology for Drug Discovery and Synthesis, Department of Chemistry, Department of Anatomy, and Department of Surgery, The University of Hong Kong, Pokfulam, Hong Kong, China
    J Proteome Res 7:2351-6. 2008
    ..These results suggest that the chromosomally encoded Cus system, which naturally controls the periplasmic copper concentrations, is selectable to confer a constitutive silver resistance phenotype...
  15. pmc Direct metal transfer between periplasmic proteins identifies a bacterial copper chaperone
    Ireena Bagai
    Department of Biochemistry and Molecular Biophysics, University of Arizona, Tucson, Arizona 85721, USA
    Biochemistry 47:11408-14. 2008
    ..These experiments establish a metallochaperone activity for CusF in the periplasm of gram-negative bacteria, serving to protect the periplasm from metal-mediated damage...
  16. pmc The iron-sulfur clusters of dehydratases are primary intracellular targets of copper toxicity
    Lee Macomber
    Department of Microbiology, University of Illinois, Urbana, IL 61801, USA
    Proc Natl Acad Sci U S A 106:8344-9. 2009
    ..Copper efflux by dedicated export systems, chelation by glutathione, and cluster repair by assembly systems all enhance the resistance of cells to this metal...
  17. doi Alternative periplasmic copper-resistance mechanisms in Gram negative bacteria
    Lucas B Pontel
    Instituto de Biologia Molecular y Celular de Rosario, Consejo Nacional de Investigaciones Cientificas y Tecnicas, Departamento de Microbiologia, Facultad de Ciencias Bioquimicas y Farmaceuticas, Universidad Nacional de Rosario, S2002LRK Rosario, Argentina
    Mol Microbiol 73:212-25. 2009
    ..coli. We posit that CueP can functionally replace the Cus complex for periplasmic copper resistance, in particular under anaerobic conditions...
  18. pmc Differences between CusA and AcrB crystallisation highlighted by protein flexibility
    Aurelien Deniaud
    Institut de Biologie Structurale Jean Pierre Ebel, UMR5075 CEA CNRS Université Joseph Fourier, Grenoble, France
    PLoS ONE 4:e6214. 2009
    Until very recently, AcrB was the only Resistance Nodulation and cell Division transporter for which the structure has been elucidated. Towards a general understanding of this protein family, CusA and AcrB were compared.
  19. pmc Effect of drug transporter genes on cysteine export and overproduction in Escherichia coli
    Satoshi Yamada
    Department of Bioscience, Fukui Prefectural University, Fukui, Japan
    Appl Environ Microbiol 72:4735-42. 2006
    ..coli on L-cysteine export and overproduction. Overexpression of the acrD, acrEF, bcr, cusA, emrAB, emrKY, ybjYZ, and yojIH genes reversed the growth inhibition of tnaA (the major CD gene)-disrupted E...
  20. pmc TolC is involved in enterobactin efflux across the outer membrane of Escherichia coli
    Corinna Bleuel
    Institut fur Mikrobiologie, Martin Luther Universität Halle, Halle, Germany, Kurt Mothes Str 3, 06120 Halle, Germany
    J Bacteriol 187:6701-7. 2005
    ..channel tunnel protein TolC but none of the respective seven resistance nodulation cell division (RND) proteins CusA, AcrB, AcrD, AcrF, MdtF (YhiV), or the twin RND MdtBC (YegNO) was essential for enterobactin export across the ..
  21. pmc Cloning and sequencing of the pheP gene, which encodes the phenylalanine-specific transport system of Escherichia coli
    J Pi
    Department of Microbiology, University of Melbourne, Parkville, Victoria, Australia
    J Bacteriol 173:3622-9. 1991
    ..coli (59.6% identity) and to a lesser extent with the yeast permeases CAN1 (arginine), PUT4 (proline), and HIP1 (histidine) of Saccharomyces cerevisiae...
  22. ncbi Characterization of the copper(II) binding site in the pink copper binding protein CusF by electron paramagnetic resonance spectroscopy
    Andrei V Astashkin
    Department of Chemistry, University of Arizona, Tucson, AZ 85721, USA
    J Biol Inorg Chem 10:221-30. 2005
    ..Apart from the two nitrogen ligands, it has been established that there are two nearby hydroxyl protons, although whether these belong to a single equatorial water ligand or two equatorial hydroxide ligands is not known...
  23. ncbi Indole induces the expression of multidrug exporter genes in Escherichia coli
    Hidetada Hirakawa
    Department of Cell Membrane Biology, Institute of Scientific and Industrial Research, Osaka University, 8 1 Mihogaoka, Ibaraki, Osaka 567 0047, Japan
    Mol Microbiol 55:1113-26. 2005
    ..BaeR and CpxR directly bound to different sequences of the acrD and mdtA promoter regions. These observations indicate that BaeR is a primary regulator, and CpxR enhances the effect of BaeR...
  24. pmc Genome-wide transcriptional response of chemostat-cultured Escherichia coli to zinc
    Lucy J Lee
    Department of Molecular Biology and Biotechnology, The University of Sheffield, Firth Court, Western Bank, Sheffield S10 2TN, United Kingdom
    J Bacteriol 187:1124-34. 2005
    ....
  25. ncbi Bacterial silver resistance: molecular biology and uses and misuses of silver compounds
    Simon Silver
    Department of Microbiology and Immunology, M C 790, University of Illinois, 835 South Wolcott Avenue, Chicago, IL 60612 7344, USA
    FEMS Microbiol Rev 27:341-53. 2003
    ..Ag is generally without adverse effects for humans, and argyria (irreversible discoloration of the skin resulting from subepithelial silver deposits) is rare and mostly of cosmetic concern...
  26. ncbi Escherichia coli mechanisms of copper homeostasis in a changing environment
    Christopher Rensing
    Department of Soil, Water, and Environmental Science, University of Arizona, Shantz Bld 38, Rm 429, Tucson, AZ 85721, USA
    FEMS Microbiol Rev 27:197-213. 2003
    ..Pathways of copper uptake and intracellular copper handling are still not identified in E. coli...
  27. ncbi Diversity of silver resistance genes in IncH incompatibility group plasmids
    A Gupta
    Department of Microbiology and Immunology, University of Illinois, M C 790, 835 S Wolcott Ave, Room E 703, Chicago, IL 60612 7344, USA
    Microbiology 147:3393-402. 2001
    ..Insertion deletions of the E. coli K-12 chromosomal homologues for silA and silP gave Ag(+) hypersensitivity for growth...
  28. pmc Genes involved in copper homeostasis in Escherichia coli
    G Grass
    Department of Soil, Water, and Environmental Science, University of Arizona, Tucson, Arizona 85721, USA
    J Bacteriol 183:2145-7. 2001
    ..In this report, data are presented that support a hypothesis that the putative multicopper oxidase CueO and the transenvelope transporter CusCFBA are involved in copper tolerance in E. coli...
  29. pmc Identification of a copper-responsive two-component system on the chromosome of Escherichia coli K-12
    G P Munson
    Department of Biochemistry, Molecular Biology, and Cell Biology, Northwestern University, Evanston, Illinois 60208 3113, USA
    J Bacteriol 182:5864-71. 2000
    ..Furthermore, the translation products of cusC and additional downstream genes are homologous to known metal ion antiporters...
  30. pmc Silver-resistant mutants of Escherichia coli display active efflux of Ag+ and are deficient in porins
    X Z Li
    Department of Microbiology, University of Saskatchewan, Saskatoon, Canada
    J Bacteriol 179:6127-32. 1997
    ..The results suggest that active efflux, presumably coded by a chromosomal gene(s), may play a major role in silver resistance, which is likely to be enhanced synergistically by decreases in OM permeability...
  31. ncbi The independent cue and cus systems confer copper tolerance during aerobic and anaerobic growth in Escherichia coli
    F W Outten
    Department of Biochemistry, Northwestern University, Evanston, Illinois 60208, USA
    J Biol Chem 276:30670-7. 2001
    ..These results underscore the increased toxicity of copper under anaerobic conditions and the complex adaptation of copper export in E. coli...