cadA

Summary

Gene Symbol: cadA
Description: lysine decarboxylase, acid-inducible
Alias: ECK4125, JW4092, ldcI
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. pmc Linkage between the bacterial acid stress and stringent responses: the structure of the inducible lysine decarboxylase
    Usheer Kanjee
    Department of Biochemistry, University of Toronto, Toronto, Ontario, Canada
    EMBO J 30:931-44. 2011
  2. pmc The Escherichia coli flagellar transcriptional activator flhD regulates cell division through induction of the acid response gene cadA
    B M Prüss
    Department of Microbiology and Immunology, University of Illinois at Chicago, 60612 7344, USA
    J Bacteriol 179:3818-21. 1997
  3. ncbi Effect of chelating agents and respiratory inhibitors on regulation of the cadA gene in Escherichia coli
    S G Reams
    Department of Microbiology, Southern Illinois University, Carbondale, IL 62901, USA
    Arch Microbiol 167:209-16. 1997
  4. ncbi Regulatory components, including integration host factor, CysB and H-NS, that influence pH responses in Escherichia coli
    R J Rowbury
    Department of Biology Darwin, University College London, UK
    Lett Appl Microbiol 24:319-28. 1997
  5. pmc Characterization of a second lysine decarboxylase isolated from Escherichia coli
    Y Kikuchi
    Central Research Laboratories, Ajinomoto Co, Inc, Kawasaki, Japan
    J Bacteriol 179:4486-92. 1997
  6. ncbi The Escherichia coli ldcC gene encodes another lysine decarboxylase, probably a constitutive enzyme
    Y Yamamoto
    Department of Genetics, Hyogo College of Medicine, Japan
    Genes Genet Syst 72:167-72. 1997
  7. pmc "Black holes" and bacterial pathogenicity: a large genomic deletion that enhances the virulence of Shigella spp. and enteroinvasive Escherichia coli
    A T Maurelli
    Department of Microbiology and Immunology, Uniformed Services University of the Health Sciences, F Edward Hebert School of Medicine, Bethesda, MD 20814 4799, USA
    Proc Natl Acad Sci U S A 95:3943-8. 1998
  8. ncbi Effects of cadA gene on cell division phenotype
    A Mandic
    Katedra za mikrobiologiju i immunologiju, Medicinski fakultet, Novi Sad
    Med Pregl 51:21-8. 1998
  9. ncbi Expression of the second lysine decarboxylase gene of Escherichia coli
    M Lemonnier
    Laboratoire de Microbiologie et Genetique Moleculaire, CNRS, Toulouse, France
    Microbiology 144:751-60. 1998
  10. ncbi RpoS-dependent expression of the second lysine decarboxylase gene in Escherichia coli
    Y Kikuchi
    Central Research Laboratories, Ajinomoto Co, Inc, Kawasaki, Japan
    Biosci Biotechnol Biochem 62:1267-70. 1998

Scientific Experts

  • Joerg Jores
  • Alfredo Torres
  • P L Moreau
  • A T Maurelli
  • Y Kikuchi
  • Orland R Gonzalez
  • B A McCormick
  • Usheer Kanjee
  • Walid A Houry
  • Jamie Snider
  • Majida El Bakkouri
  • Eftichia Alexopoulos
  • Irina Gutsche
  • Boyu Zhao
  • Emil F Pai
  • V I Chalova
  • Manas K Chattopadhyay
  • Georg Fritz
  • Kirsten Jung
  • Christoph Kuper
  • Waraporn Soksawatmaekhin
  • Mariassunta Casalino
  • M N Neely
  • W A Day
  • Shaliny Ramachandran
  • Kaiyin Liu
  • Guillaume Thibault
  • Gael Goret
  • Wim P Burmeister
  • Miao Yu
  • Guy Schoehn
  • Larissa Tetsch
  • Celia White Tabor
  • Herbert Tabor
  • C L Woodward
  • Ulrich Gerland
  • Korinna Burdack
  • Ina Haneburger
  • Christiane Koller
  • S C Ricke
  • H Samartzidou
  • M Lemonnier
  • E R Olson
  • Sabulal Baby
  • Michelle Lin
  • Gareth Butland
  • A Mandic
  • Brian Cox
  • Jack Greenblatt
  • Andrew Emili
  • X Shi
  • Kaori Sakata
  • Y Yamamoto
  • B M Prüss
  • R J Rowbury
  • Aiko Kuraishi
  • Kazuei Igarashi
  • G N Bennett
  • Keiko Kashiwagi
  • S G Reams
  • Maria Carmela Latella
  • Gianni Prosseda
  • Bianca Colonna
  • C L Dell
  • Y K Park
  • R E Fernandez
  • S Y Meng
  • A H Delcour
  • M Takayama
  • D Lane
  • H Ohmori
  • J Furuyama
  • Y Miwa
  • D Markovic
  • D P Clark
  • P Matsumura
  • N Lee
  • F Mat-Jan
  • K Miyoshi
  • J W Foster
  • B Bearson
  • I S Bang
  • S H Bang
  • T Ohyama
  • K Igarashi
  • E A Auger
  • D L Sabo
  • N Watson
  • H Kobayashi
  • B C Waasdorp

Detail Information

Publications52

  1. pmc Linkage between the bacterial acid stress and stringent responses: the structure of the inducible lysine decarboxylase
    Usheer Kanjee
    Department of Biochemistry, University of Toronto, Toronto, Ontario, Canada
    EMBO J 30:931-44. 2011
    The Escherichia coli inducible lysine decarboxylase, LdcI/CadA, together with the inner-membrane lysine-cadaverine antiporter, CadB, provide cells with protection against mild acidic conditions (pH∼5)...
  2. pmc The Escherichia coli flagellar transcriptional activator flhD regulates cell division through induction of the acid response gene cadA
    B M Prüss
    Department of Microbiology and Immunology, University of Illinois at Chicago, 60612 7344, USA
    J Bacteriol 179:3818-21. 1997
    FlhD is a positive regulator of cadA. A mutant with a transposon-mediated lacZ fusion to cadA exhibited a cell division phenotype similar to that of the flhD mutant and had FlhD-dependent beta-galactosidase activity...
  3. ncbi Effect of chelating agents and respiratory inhibitors on regulation of the cadA gene in Escherichia coli
    S G Reams
    Department of Microbiology, Southern Illinois University, Carbondale, IL 62901, USA
    Arch Microbiol 167:209-16. 1997
    The cadA gene that encodes lysine decarboxylase in Escherichia coli is induced by low pH and - during anaerobic growth - by the substrate, lysine...
  4. ncbi Regulatory components, including integration host factor, CysB and H-NS, that influence pH responses in Escherichia coli
    R J Rowbury
    Department of Biology Darwin, University College London, UK
    Lett Appl Microbiol 24:319-28. 1997
    ..H-NS, IHF and CysB; acid induction of LysU has similar requirements but also needs Lrp; lysine decarboxylase (CadA) formation at acid pH is controlled quite differently, needing the CadC activator and interaction of lysine/lysine ..
  5. pmc Characterization of a second lysine decarboxylase isolated from Escherichia coli
    Y Kikuchi
    Central Research Laboratories, Ajinomoto Co, Inc, Kawasaki, Japan
    J Bacteriol 179:4486-92. 1997
    ..The hybridization experiments with a cadA probe at low stringency showed that the homologous region of cadA was located in lambda Kohara phage clone 6F5 at 4...
  6. ncbi The Escherichia coli ldcC gene encodes another lysine decarboxylase, probably a constitutive enzyme
    Y Yamamoto
    Department of Genetics, Hyogo College of Medicine, Japan
    Genes Genet Syst 72:167-72. 1997
    ..coli (CadA, acid-inducible LDC, 715 aa) or from Hafnia alvei (739 aa)...
  7. pmc "Black holes" and bacterial pathogenicity: a large genomic deletion that enhances the virulence of Shigella spp. and enteroinvasive Escherichia coli
    A T Maurelli
    Department of Microbiology and Immunology, Uniformed Services University of the Health Sciences, F Edward Hebert School of Medicine, Bethesda, MD 20814 4799, USA
    Proc Natl Acad Sci U S A 95:3943-8. 1998
    ..coli strains but is uniformly absent in Shigella strains. When the gene for LDC, cadA, was introduced into Shigella flexneri 2a, virulence became attenuated, and enterotoxin activity was inhibited ..
  8. ncbi Effects of cadA gene on cell division phenotype
    A Mandic
    Katedra za mikrobiologiju i immunologiju, Medicinski fakultet, Novi Sad
    Med Pregl 51:21-8. 1998
    Studying cell division regulatory mechanisms in Escherichia coli an important role of cad operon was discovered. The cadA gene is part of the cad operon...
  9. ncbi Expression of the second lysine decarboxylase gene of Escherichia coli
    M Lemonnier
    Laboratoire de Microbiologie et Genetique Moleculaire, CNRS, Toulouse, France
    Microbiology 144:751-60. 1998
    ..In the case of lysine, an inducible decarboxylase (CadA) has been extensively characterized, but evidence for the existence of a second lysine decarboxylase is fragmentary ..
  10. ncbi RpoS-dependent expression of the second lysine decarboxylase gene in Escherichia coli
    Y Kikuchi
    Central Research Laboratories, Ajinomoto Co, Inc, Kawasaki, Japan
    Biosci Biotechnol Biochem 62:1267-70. 1998
    ..J. Baceriol. 179, 4486-4492 (1997)]. This report showes that the expression of ldc as well as cadA was induced at stationary phase in the wild type of E. coli...
  11. pmc Excretion of endogenous cadaverine leads to a decrease in porin-mediated outer membrane permeability
    H Samartzidou
    Department of Biology and Biochemistry, University of Houston, Houston, Texas 77204 5513, USA
    J Bacteriol 181:791-8. 1999
    ..The results presented here support the notion that polyamines can act as endogenous modulators of outer membrane permeability, possibly as part of an adaptive response to acidic conditions...
  12. ncbi Inhibition of Shigella flexneri-induced transepithelial migration of polymorphonuclear leucocytes by cadaverine
    B A McCormick
    Department of Pediatric Gastroenterology and Nutrition, Massachusetts General Hospital East, Charlestown 02129, USA
    Cell Microbiol 1:143-55. 1999
    ..An example is cadA (encoding lysine decarboxylase), which is uniformly absent in Shigella spp...
  13. pmc Pathoadaptive mutations that enhance virulence: genetic organization of the cadA regions of Shigella spp
    W A Day
    Department of Microbiology and Immunology, F Edward Hebert School of Medicine, Uniformed Services University of the Health Sciences, Bethesda, Maryland 20814 4799, USA
    Infect Immun 69:7471-80. 2001
    ..the product of LDC activity, cadaverine, blocks the action of Shigella enterotoxins and that the gene encoding LDC, cadA, was abolished by large chromosomal deletions in each Shigella species...
  14. pmc CadC is the preferential target of a convergent evolution driving enteroinvasive Escherichia coli toward a lysine decarboxylase-defective phenotype
    Mariassunta Casalino
    Dipartimento di Biologia, Universita Roma Tre, 00146 Rome, Italy
    Infect Immun 71:5472-9. 2003
    ..Comparative analysis between the cad regions of S. flexneri and EIEC suggests that the LDC(-) phenotype has been attained by different strategies within the E. coli species...
  15. ncbi Excretion and uptake of cadaverine by CadB and its physiological functions in Escherichia coli
    Waraporn Soksawatmaekhin
    Graduate School of Pharmaceutical Sciences, Chiba University, 1 33 Yayoi cho, Inage Ku, Chiba 263 8522, Japan
    Mol Microbiol 51:1401-12. 2004
    ..At acidic pH, the operon containing cadB and cadA, encoding lysine decarboxylase, was induced in the presence of lysine...
  16. ncbi Formation of a distinctive complex between the inducible bacterial lysine decarboxylase and a novel AAA+ ATPase
    Jamie Snider
    Department of Biochemistry, University of Toronto, Toronto, Ontario M5S 1A8, Canada
    J Biol Chem 281:1532-46. 2006
    ..Importantly, we demonstrate that RavA interacts strongly with inducible lysine decarboxylase (LdcI or CadA) forming a large cage-like structure consisting of two LdcI decamers linked by a maximum of five RavA ..
  17. ncbi CadC-mediated activation of the cadBA promoter in Escherichia coli
    Christoph Kuper
    Department of Biology I, Microbiology, Ludwig Maximilians University Munich, Munich, Germany
    J Mol Microbiol Biotechnol 10:26-39. 2005
    ..a member of the ToxR-like proteins, activates transcription of the cadBA operon encoding the lysine decarboxylase CadA and the lysine-cadaverine antiporter CadB...
  18. ncbi Identification and characterization of "pathoadaptive mutations" of the cadBA operon in several intestinal Escherichia coli
    Joerg Jores
    Institut für Mikrobiologie und Tierseuchen, Freie Universitat Berlin, Philippstrasse 13, D 10115 Berlin, Germany
    Int J Med Microbiol 296:547-52. 2006
    ..and EIEC strains. Interestingly, the alterations of the cad genes were similar to those previously reported, pointing towards a parallel evolution of LDC silencing in different intestinal E. coli pathotypes...
  19. ncbi Parameter estimation using Simulated Annealing for S-system models of biochemical networks
    Orland R Gonzalez
    Department of Computer Science University of the Philippines Diliman, Munich, Germany
    Bioinformatics 23:480-6. 2007
    ..Retrieving this information will require systematic application of both experimental and computational methods...
  20. pmc The lysine decarboxylase CadA protects Escherichia coli starved of phosphate against fermentation acids
    Patrice L Moreau
    Laboratoire de Chimie Bacterienne, CNRS LCB, Marseille, France
    J Bacteriol 189:2249-61. 2007
    ..b>CadA plays a key role in the defense of Salmonella at pH 3 but was thought to be ineffective in Escherichia coli since ..
  21. doi The cad locus of Enterobacteriaceae: more than just lysine decarboxylation
    Alfredo G Torres
    Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, 77555, USA
    Anaerobe 15:1-6. 2009
    ....
  22. pmc Crystallization and preliminary X-ray analysis of the inducible lysine decarboxylase from Escherichia coli
    Eftichia Alexopoulos
    Department of Biochemistry, University of Toronto, 1 King s College Circle, Medical Sciences Building, Toronto, Ontario M5S1A8, Canada
    Acta Crystallogr Sect F Struct Biol Cryst Commun 64:700-6. 2008
    The decameric inducible lysine decarboxylase (LdcI) from Escherichia coli has been crystallized in space groups C2 and C222(1); the Ta6Br12(2+) cluster was used to derivatize the C2 crystals...
  23. doi Induction of cadBA in an Escherichia coli lysine auxotroph transformed with a cad-gfp transcriptional fusion
    V I Chalova
    Department of Poultry Science, Texas A and M University, College Station, TX 77843, USA
    Antonie Van Leeuwenhoek 95:305-10. 2009
    ..08 micromol/g cell dry weight). For the latter, the detectable level of induction was delayed for 1 h but the maximum level of induction response was higher...
  24. pmc Polyamines are not required for aerobic growth of Escherichia coli: preparation of a strain with deletions in all of the genes for polyamine biosynthesis
    Manas K Chattopadhyay
    Laboratory of Biochemistry and Genetics, National Institute of Diabetes, Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Bacteriol 191:5549-52. 2009
    ..spe D (adenosylmethionine decarboxylase), speE (spermidine synthase), speF (inducible ornithine decarboxylase), cadA (lysine decarboxylase), and ldcC (lysine decarboxylase)--had been deleted...
  25. doi Induction kinetics of a conditional pH stress response system in Escherichia coli
    Georg Fritz
    Arnold Sommerfeld Center for Theoretical Physics, Ludwig Maximilians Universitat Munchen, Germany
    J Mol Biol 393:272-86. 2009
    ..Furthermore, the analysis puts causal constraints on the precise mechanism of signal transduction via the regulatory protein CadC...
  26. pmc Structure of RavA MoxR AAA+ protein reveals the design principles of a molecular cage modulating the inducible lysine decarboxylase activity
    Majida El Bakkouri
    Department of Biochemistry, University of Toronto, Toronto, ON, Canada M5S 1A8
    Proc Natl Acad Sci U S A 107:22499-504. 2010
    ..coli MoxR protein, RavA (Regulatory ATPase variant A), tightly interacts with the inducible lysine decarboxylase, LdcI/CadA, to form a unique cage-like structure...
  27. pmc Identification of elements involved in transcriptional regulation of the Escherichia coli cad operon by external pH
    N Watson
    Department of Molecular Biology, Upjohn Company, Kalamazoo, Michigan 49001
    J Bacteriol 174:530-40. 1992
    Expression of the lysine decarboxylase gene (cadA) of Escherichia coli is induced upon external acidification. To dissect the molecular mechanisms responsible for this regulation, we analyzed a 4.2-kbp region upstream from cadA...
  28. pmc Nucleotide sequence of the Escherichia coli cad operon: a system for neutralization of low extracellular pH
    S Y Meng
    Department of Biochemistry and Cell Biology, Rice University, Houston, Texas 77251
    J Bacteriol 174:2659-69. 1992
    ..of Escherichia coli has been the subject of enzymological studies, and the gene encoding lysine decarboxylase (cadA) and a regulatory gene (cadR) have been mapped...
  29. pmc Regulation of the Escherichia coli cad operon: location of a site required for acid induction
    S Y Meng
    Department of Biochemistry and Cell Biology, Rice University, Houston, Texas 77251
    J Bacteriol 174:2670-8. 1992
    ..Deletion analysis of the upstream region and cloning of various fragments to make cadA::lacZ or cadB::lacZ protein fusions or operon fusions showed that cadA was translated more efficiently than cadB ..
  30. ncbi Construction of lac fusions to the inducible arginine- and lysine decarboxylase genes of Escherichia coli K12
    E A Auger
    Department of Biochemistry, Rice University, Houston, Texas 77251
    Mol Microbiol 3:609-20. 1989
    ..The position of the Mudlac fusion in lysine decarboxylase-deficient strains has been mapped to the cadA gene at 93...
  31. ncbi Regulation of lysine decarboxylase activity in Escherichia coli K-12
    E A Auger
    Department of Biochemistry, Rice University, Houston, TX 77251
    Arch Microbiol 151:466-8. 1989
    ..The results suggested that the pH and air responses were independent and that these known regulatory processes are not responsible for this regulation of the biodegradative lysine decarboxylase...
  32. ncbi Chemical properties of Escherichia coli lysine decarboxylase including a segment of its pyridoxal 5'-phosphate binding site
    D L Sabo
    Biochemistry 13:670-6. 1974
  33. ncbi Purification and physical properties of inducible Escherichia coli lysine decarboxylase
    D L Sabo
    Biochemistry 13:662-70. 1974
  34. ncbi Mass screening for mutants in the biosynthetic pathway for polyamines in Escherichia coli: a general method for mutants in enzymatic reactions producing CO2
    C W Tabor
    Methods Enzymol 94:83-91. 1983
  35. pmc Escherichia coli regulatory mutation affecting lysine transport and lysine decarboxylase
    P S Popkin
    J Bacteriol 141:485-92. 1980
    ..All thiosine-resistant recombinants assayed showed reduced lysine transport. In many of these recombinants the derepression of lysine decarboxylase was not expressed...
  36. ncbi [Conditions of the synthesis of lysine decarboxylase by Escherichia coli MRE 600]
    S Ia Beretskene
    Prikl Biokhim Mikrobiol 16:351-5. 1980
    ..0 at 29--30 degrees C. Aeration increased the yield by about 3 times and decreased LDC activity by 5.5 times...
  37. pmc Construction of an Escherichia coli strain unable to synthesize putrescine, spermidine, or cadaverine: characterization of two genes controlling lysine decarboxylase
    H Tabor
    J Bacteriol 144:952-6. 1980
    ..rule out the possibility that the presence of cadaverine permitted the growth of this strain, we isolated a mutant (cadA) that is deficient in cadaverine biosynthesis, namely, a mutant lacking lysine decarboxylase, and transduced this ..
  38. ncbi Altered pH and lysine signalling mutants of cadC, a gene encoding a membrane-bound transcriptional activator of the Escherichia coli cadBA operon
    C L Dell
    Department of Biotechnology, Parke Davis Pharmaceutical Research, Division of Warner Lambert Co, Ann Arbor, Michigan 48105
    Mol Microbiol 14:7-16. 1994
    ..6). Eight cadC mutants were isolated based on their ability to confer expression of a cadA-lacZ fusion independent of low external pH or exogenous lysine...
  39. pmc Effects of multicopy LeuO on the expression of the acid-inducible lysine decarboxylase gene in Escherichia coli
    X Shi
    Department of Biochemistry and Cell Biology, Rice University, Houston, Texas 77251
    J Bacteriol 177:810-4. 1995
    ..that mutations in hns, the structural gene for the histone-like protein H-NS, cause derepressed expression of cadA, which encodes the acid-inducible lysine decarboxylase at noninducing pH (pH 8.0)...
  40. ncbi Escherichia coli cad operon functions as a supplier of carbon dioxide
    M Takayama
    Faculty of Pharmaceutical Sciences, Chiba University, Japan
    Mol Microbiol 11:913-8. 1994
    We examined the gene expression of the Escherichia coli cad operon, which consisted of the genes cadB and cadA (lysine decarboxylase), using cells possessing cadB-lacZ fusion gene...
  41. pmc Roles of LysP and CadC in mediating the lysine requirement for acid induction of the Escherichia coli cad operon
    M N Neely
    Parke Davis Pharmaceutical Research, Division of Warner Lambert Co, Ann Arbor, Michigan 48105
    J Bacteriol 176:3278-85. 1994
    ..To better understand the nature of the lysine-dependent signal, mutants were isolated which expressed a cadA-lacZ transcription fusion in the absence of lysine while retaining pH regulation...
  42. pmc Modulation of acid-induced amino acid decarboxylase gene expression by hns in Escherichia coli
    X Shi
    Department of Biochemistry and Cell Biology, Rice University, Houston, Texas 77251
    J Bacteriol 175:1182-6. 1993
    Biodegradative arginine decarboxylase and lysine decarboxylase, encoded by adi and cadA, respectively, are induced to maximal levels when Escherichia coli is grown anaerobically in rich medium at acidic pH...
  43. ncbi Internal pH crisis, lysine decarboxylase and the acid tolerance response of Salmonella typhimurium
    Y K Park
    Department of Biology, Korea University, Soeul, Korea
    Mol Microbiol 20:605-11. 1996
    ..The results suggest that S. typhimurium has a variety of means of fulfilling the pH homeostasis requirement of the ATR in the form of inducible amino acid decarboxylases...
  44. pmc Kinetics of expression of the Escherichia coli cad operon as a function of pH and lysine
    M N Neely
    Department of Biotechnology, Parke Davis Pharmaceutical Research, Warner Lambert Inc, Ann Arbor, Michigan 48105, USA
    J Bacteriol 178:5522-8. 1996
    ..Together, these data suggest that CadC senses the lysine- and pH-induced signals separately and that one of the roles of lysine in inducing cadBA may be to repress expression of lysP, thus eliminating the repressing effects of LysP...