ampC

Summary

Gene Symbol: ampC
Description: penicillin-binding protein; beta-lactamase, intrinsically weak
Alias: ECK4146, JW4111, ampA
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. pmc Dissemination of Extended-Spectrum β-Lactamase- and AmpC β-Lactamase-Producing Escherichia coli within the Food Distribution System of Ho Chi Minh City, Vietnam
    Do Phuc Nguyen
    Institute of Public Health, Ministry of Health, 159 Hung Phu Street, Ward 8, District 8, Ho Chi Minh City 700000, Vietnam
    Biomed Res Int 2016:8182096. 2016
  2. doi Clinical management of infections caused by Enterobacteriaceae that express extended-spectrum β-lactamase and AmpC enzymes
    Patrick N A Harris
    Infection and Immunity Theme, University of Queensland Centre for Clinical Research, Brisbane, Queensland, Australia
    Semin Respir Crit Care Med 36:56-73. 2015
  3. doi Diversity of bla genes and low incidence of CTX-M in plasmid-mediated AmpC-producing Escherichia coli clinical isolates
    Fatima Galan-Sanchez
    Service of Microbiology, Puerta del Mar University Hospital, Cadiz, Spain
    APMIS 122:796-9. 2014
  4. ncbi Deconstructing fragment-based inhibitor discovery
    Kerim Babaoglu
    Nat Chem Biol 2:720-3. 2006
  5. pmc Comprehensive mechanistic analysis of hits from high-throughput and docking screens against beta-lactamase
    Kerim Babaoglu
    Department of Pharmaceutical Chemistry, University of California San Francisco, San Francisco, California 94158 2330, USA
    J Med Chem 51:2502-11. 2008
  6. pmc Docking for fragment inhibitors of AmpC beta-lactamase
    Denise G Teotico
    Department of Pharmaceutical Chemistry, University of California, 1700 4th Street, MC 2550, San Francisco, CA 94158, USA
    Proc Natl Acad Sci U S A 106:7455-60. 2009
  7. doi Extended-spectrum-β-lactamase- and AmpC-β-lactamase-producing Escherichia coli in Dutch broilers and broiler farmers
    Cindy Dierikx
    Central Veterinary Institute of Wageningen UR, Department of Bacteriology and TSEs, PO Box 65, 8200 AB Lelystad, The Netherlands
    J Antimicrob Chemother 68:60-7. 2013
  8. pmc Structural bases for stability-function tradeoffs in antibiotic resistance
    Veena L Thomas
    Graduate Program in Pharmaceutical Sciences and Pharmacogenomics, University of California San Francisco, San Francisco, CA 94158 2518, USA
    J Mol Biol 396:47-59. 2010
  9. doi Extended-spectrum β-lactamase-producing Escherichia coli from retail chicken meat and humans: comparison of strains, plasmids, resistance genes, and virulence factors
    Jan A J W Kluytmans
    Laboratory for Medical Microbiology and Infection Control, Amphia Hospital, Breda, The Netherlands
    Clin Infect Dis 56:478-87. 2013
  10. pmc Contributions of the AmpC beta-lactamase and the AcrAB multidrug efflux system in intrinsic resistance of Escherichia coli K-12 to beta-lactams
    A Mazzariol
    Institute of Microbiology, University of Verona, Verona, Italy
    Antimicrob Agents Chemother 44:1387-90. 2000

Research Grants

  1. Plasmid-Mediated Quinolone Resistance
    George Jacoby; Fiscal Year: 2007

Scientific Experts

  • Cindy Dierikx
  • Stéphane Corvec
  • George Jacoby
  • K D Young
  • Thierry Naas
  • Patrice Nordmann
  • Jan A J W Kluytmans
  • Brian K Shoichet
  • Kerim Babaoglu
  • Hedi Mammeri
  • Do Phuc Nguyen
  • Patrick N A Harris
  • Fatima Galan-Sanchez
  • Veena L Thomas
  • R A Powers
  • Denise G Teotico
  • Yu Chen
  • Maja Babic
  • Mohammad Arifuzzaman
  • M Paola Costi
  • Dobryan M Tracz
  • Laurent Poirel
  • T A Henderson
  • Nigel P Brenwald
  • Valentina Buzzoni
  • B K Shoichet
  • Yohei Doi
  • K Nasim
  • Jesus Blazquez
  • Yoshimasa Yamamoto
  • Shinya Yonogi
  • Yuko Kumeda
  • Nguyen Minh Doan Tran
  • Thanh Phong Ngo
  • Hidetada Hirakawa
  • Thi Anh Dao Nguyen
  • Ryuji Kawahara
  • Federica Morandi
  • Thi Hien Le
  • Takao Kawai
  • Masashi Kanki
  • Michio Jinnai
  • Yuji Hirai
  • Van Chinh Dang
  • S A Denome
  • Jorge M Santos
  • Beth M Beadle
  • Pilar Aznar-Marín
  • Pilar Marín-Casanova
  • Manuel Rodríguez-Iglesias
  • Andrea C McReynolds
  • N Caroff
  • A Mazzariol
  • R E Bishop
  • Rafaela S Ferreira
  • Gabriel J Rocklin
  • Anthony M Giannetti
  • Andrea McReynolds
  • Laura Cancian
  • David A Maltby
  • Brian Feng
  • Michael E Nelson
  • Ajit Jadhav
  • John J Irwin
  • Christopher P Austin
  • James Inglese
  • Craig J Thomas
  • Anton Simeonov
  • G S Weston
  • P K Elf
  • Mohammad Altaf-Ul-Amin
  • Takeshi Ara
  • Kenji Nakahigashi
  • Chiharu Takita
  • Taku Oshima
  • Kohei Tsuzuki
  • Hsuan Cheng Huang
  • Shigehiko Kanaya
  • Nicolas Fortineau
  • Aki Hirai
  • Masaru Tomita
  • Tomoya Baba
  • Seira Nakamura
  • Aya Itoh
  • Hirotada Mori
  • Robert A Bonomo
  • Rintaro Saito
  • Tomoyo Kawamura
  • Kensaku Nishikata
  • Andrea M Hujer

Detail Information

Publications57

  1. pmc Dissemination of Extended-Spectrum β-Lactamase- and AmpC β-Lactamase-Producing Escherichia coli within the Food Distribution System of Ho Chi Minh City, Vietnam
    Do Phuc Nguyen
    Institute of Public Health, Ministry of Health, 159 Hung Phu Street, Ward 8, District 8, Ho Chi Minh City 700000, Vietnam
    Biomed Res Int 2016:8182096. 2016
    ..5% and 50.8%, resp.). These findings demonstrate that animal-based food products in HCMC represent a major reservoir of ESBL/pAmpC-producing E. coli. ..
  2. doi Clinical management of infections caused by Enterobacteriaceae that express extended-spectrum β-lactamase and AmpC enzymes
    Patrick N A Harris
    Infection and Immunity Theme, University of Queensland Centre for Clinical Research, Brisbane, Queensland, Australia
    Semin Respir Crit Care Med 36:56-73. 2015
    ..Some bacterial species possess chromosomally encoded broad-spectrum cephalosporinases (AmpC) that may be expressed at high level by mutational loss of regulatory genes and are intrinsic in some common ..
  3. doi Diversity of bla genes and low incidence of CTX-M in plasmid-mediated AmpC-producing Escherichia coli clinical isolates
    Fatima Galan-Sanchez
    Service of Microbiology, Puerta del Mar University Hospital, Cadiz, Spain
    APMIS 122:796-9. 2014
    The aim of this study was to characterize plasmid-mediated AmpC (pAmpC)-producing Escherichia coli clinical isolates. A total of 101 strains with AmpC-susceptibility pattern were prospectively included...
  4. ncbi Deconstructing fragment-based inhibitor discovery
    Kerim Babaoglu
    Nat Chem Biol 2:720-3. 2006
    ....
  5. pmc Comprehensive mechanistic analysis of hits from high-throughput and docking screens against beta-lactamase
    Kerim Babaoglu
    Department of Pharmaceutical Chemistry, University of California San Francisco, San Francisco, California 94158 2330, USA
    J Med Chem 51:2502-11. 2008
    ..Structure-based methods may prioritize weak-but-novel chemotypes in unbiased library screens...
  6. pmc Docking for fragment inhibitors of AmpC beta-lactamase
    Denise G Teotico
    Department of Pharmaceutical Chemistry, University of California, 1700 4th Street, MC 2550, San Francisco, CA 94158, USA
    Proc Natl Acad Sci U S A 106:7455-60. 2009
    ..The ability of docking to prioritize these fragments suggests that the technique can be used to exploit the better chemotype coverage that exists at the fragment level...
  7. doi Extended-spectrum-β-lactamase- and AmpC-β-lactamase-producing Escherichia coli in Dutch broilers and broiler farmers
    Cindy Dierikx
    Central Veterinary Institute of Wageningen UR, Department of Bacteriology and TSEs, PO Box 65, 8200 AB Lelystad, The Netherlands
    J Antimicrob Chemother 68:60-7. 2013
    The aim of this study was to establish the prevalence of extended-spectrum β-lactamase (ESBL)- and AmpC β-lactamase-producing Escherichia coli at Dutch broiler farms and in farmers and to compare ESBL/AmpC-producing isolates from ..
  8. pmc Structural bases for stability-function tradeoffs in antibiotic resistance
    Veena L Thomas
    Graduate Program in Pharmaceutical Sciences and Pharmacogenomics, University of California San Francisco, San Francisco, CA 94158 2518, USA
    J Mol Biol 396:47-59. 2010
    ..These results explain the structural bases for the antibiotic resistance conferred by these substitutions and their corresponding decrease in protein stability, which will constrain the evolution of new antibiotic resistance...
  9. doi Extended-spectrum β-lactamase-producing Escherichia coli from retail chicken meat and humans: comparison of strains, plasmids, resistance genes, and virulence factors
    Jan A J W Kluytmans
    Laboratory for Medical Microbiology and Infection Control, Amphia Hospital, Breda, The Netherlands
    Clin Infect Dis 56:478-87. 2013
    ..Now, we have extended the analysis with plasmid replicons, virulence factors, and highly discriminatory genomic profiling methods...
  10. pmc Contributions of the AmpC beta-lactamase and the AcrAB multidrug efflux system in intrinsic resistance of Escherichia coli K-12 to beta-lactams
    A Mazzariol
    Institute of Microbiology, University of Verona, Verona, Italy
    Antimicrob Agents Chemother 44:1387-90. 2000
    The roles of the AmpC chromosomal beta-lactamase and the AcrAB efflux system in levels of intrinsic resistance and susceptibility of Escherichia coli to beta-lactams were studied with a set of isogenic strains...
  11. ncbi Analysis of the effects of -42 and -32 ampC promoter mutations in clinical isolates of Escherichia coli hyperproducing ampC
    N Caroff
    Laboratoire de Bacteriologie virologie, Faculte de Pharmacie, 1 rue Gaston Veil, 44035 Nantes Laboratoire de Bactériologie, Virologie, Hygiene Hospitaliere, CHU, Nantes, France
    J Antimicrob Chemother 45:783-8. 2000
    Escherichia coli usually produces only very small amounts of a constitutive AmpC beta-lactamase, but clinical strains overproducing this enzyme have been isolated. Three different ampC promoters of E...
  12. ncbi Approaching the physiological functions of penicillin-binding proteins in Escherichia coli
    K D Young
    Department of Microbiology and Immunology, School of Medicine and Health Sciences, University of North Dakota, Grand Forks 58202 9037, USA
    Biochimie 83:99-102. 2001
    ....
  13. ncbi The gene bolA regulates dacA (PBP5), dacC (PBP6) and ampC (AmpC), promoting normal morphology in Escherichia coli
    Jorge M Santos
    Instituto de Technologia Química e Biológica, Universidade Nova de Lisboa, Oeiras, Portugal
    Mol Microbiol 45:1729-40. 2002
    ..The results show that bolA controls the levels of transcription of dacA (PBP5), dacC (PBP6) and ampC (AmpC), a class C beta-lactamase, thus connecting for the first time penicillin binding proteins (PBPs) and beta-..
  14. ncbi Beta-lactam resistance modulated by the overexpression of response regulators of two-component signal transduction systems in Escherichia coli
    Hidetada Hirakawa
    Department of Cell Membrane Biology, Institute of Scientific and Industrial Research, Osaka University, Ibaraki, Osaka 567 0047, Japan
    J Antimicrob Chemother 52:576-82. 2003
    ..We cloned all 32 response regulators and examined whether or not response regulator-overexpressing cells confer resistance to beta-lactam antibiotics in E. coli...
  15. ncbi Comparison of two RT-PCR methods for quantifying ampC specific transcripts in Escherichia coli strains
    Stéphane Corvec
    Laboratoire de Bacteriologie Virologie Hygiene Hospitaliere, Institut de Biologie des Hopitaux de Nantes, 9 quai Moncousu, 44093 Nantes Cedex 01, France
    FEMS Microbiol Lett 228:187-91. 2003
    In Escherichia coli, beta-lactam resistance usually depends on beta-lactamase production. AmpC chromosomal cephalosporinase hyperproduction is generally due to mutations in the ampC gene promoter. In order to study ampC expression in E...
  16. ncbi Aza-boronic acids as non-beta-lactam inhibitors of AmpC-beta-lactamase
    Valentina Buzzoni
    Dipartimento di Scienze Farmaceutiche, Universita degli Studi di Modena e Reggio Emilia, Via Campi 183, 41100 Modena, Italy
    Bioorg Med Chem Lett 14:3979-83. 2004
    With the aim of improving the ability of non-beta-lactam inhibitors to inhibit AmpC-beta-lactamase, a series of 3-aza-phenyl-boronic acid derivatives was obtained using in parallel synthesis...
  17. pmc New method for laboratory detection of AmpC beta-lactamases in Escherichia coli and Klebsiella pneumoniae
    K Nasim
    Department of Pathology and Laboratory Medicine, University of Calgary, Calgary, Alberta, Canada
    J Clin Microbiol 42:4799-802. 2004
    ..assay was compared to the previously published modified three-dimensional (M3D) assay for the detection of AmpC production in Escherichia coli and Klebsiella pneumoniae. Clinical isolates of cefoxitin-resistant E...
  18. ncbi Increase in ampC promoter strength due to mutations and deletion of the attenuator in a clinical isolate of cefoxitin-resistant Escherichia coli as determined by RT-PCR
    Dobryan M Tracz
    National Microbiology Laboratory, Public Health Agency of Canada, 1015 Arlington St, Winnipeg, Manitoba, Canada, R3E 3R2
    J Antimicrob Chemother 55:768-72. 2005
    ..To characterize the mechanism of cefoxitin resistance in clinical isolate Escherichia coli N99-0001...
  19. ncbi Disc methods for detecting AmpC {beta}-lactamase-producing clinical isolates of Escherichia coli and Klebsiella pneumoniae
    Nigel P Brenwald
    J Antimicrob Chemother 56:600-1. 2005
  20. pmc Resistance of Escherichia coli to Penicillins I. Genetic Study of Some Ampicillin-Resistant Mutants
    K G Eriksson-Grennberg
    The Molecular Biology Group, Institute of Biochemistry, University of Uppsala, Uppsala, Sweden
    J Bacteriol 90:54-62. 1965
    ..which are moderately resistant to ampicillin (Amp(r) (10)) carry mutations in a locus which we have designated ampA; another strain, which is resistant to high levels (50 mug/ml) of ampicillin, is a multistep mutant for which a ..
  21. ncbi What's new in antibiotic resistance? Focus on beta-lactamases
    Maja Babic
    Division of Infectious Diseases, Department of Medicine, University Hospitals of Cleveland, USA
    Drug Resist Updat 9:142-56. 2006
    ..Despite many years studying beta-lactamases, important clinical and scientific questions still remain...
  22. pmc Escherichia coli K-12 mutants hyperproducing chromosomal beta-lactamase by gene repetitions
    S Normark
    J Bacteriol 132:912-22. 1977
    ..mutants hyperproducing chromosomal beta-lactamase arose spontaneously from strains carrying ampA1 ampC(+). Such mutants were found even in a recA background. Two Amp(r)-100 strains were analyzed genetically...
  23. doi In vivo selection of an Escherichia coli isolate highly resistant to ciprofloxacin and ceftazidime: role of a 4-bp duplication in acrR and ampC overexpression
    Stéphane Corvec
    Int J Antimicrob Agents 32:196-8. 2008
  24. pmc AmpC beta-lactamases
    George A Jacoby
    Lahey Clinic, Burlington, Massachusetts 01805, USA
    Clin Microbiol Rev 22:161-82, Table of Contents. 2009
    b>AmpC beta-lactamases are clinically important cephalosporinases encoded on the chromosomes of many of the Enterobacteriaceae and a few other organisms, where they mediate resistance to cephalothin, cefazolin, cefoxitin, most penicillins, ..
  25. pmc Re-examining the role of Lys67 in class C beta-lactamase catalysis
    Yu Chen
    Department of Pharmaceutical Chemistry, University of California San Francisco, San Francisco, California 94158 2550, USA
    Protein Sci 18:662-9. 2009
    ..Using the Class C beta-lactamase AmpC, we have reinvestigated the activity of this K67R mutant enzyme, using biochemical and structural studies...
  26. ncbi The E. coli beta-lactamase attenuator mediates growth rate-dependent regulation
    B Jaurin
    Nature 290:221-5. 1981
    ..A single base alteration within its attenuator led to a loss in the cell's ability to coordinate its content of beta-lactamase with growth rate. We suggest a mechanism through which this mode of regulation operates...
  27. pmc Resistance of Escherichia coli to penicillins: fine-structure mapping and dominance of chromosomal beta-lactamase mutations
    S Normark
    J Bacteriol 132:1-7. 1977
    ..Bacteriol. 116:123-130, 1973) were shown to have occurred in the structural gene for beta-lactamase, designated ampC. It is suggested that all beta-lactamase-negative mutants studied here were altered in ampC...
  28. ncbi Isolation and characterization of DNA repetitions carrying the chromosomal beta-lactamase gene of Escherichia coli K-12
    T Edlund
    Mol Gen Genet 173:115-25. 1979
    ..The physical location of ampC within this plasmid was further deduced by in vitro cloning...
  29. ncbi Production of a soluble form of fumarate reductase by multiple gene duplication in Escherichia coli K12
    S T Cole
    Eur J Biochem 102:65-71. 1979
    1. Ampicillin-hyperresistant mutants of Escherichia coli K12 bearing multiple gene duplications in the ampC (beta-lactamase) gene region of the chromosome overproduced at least six proteins with molecular weights 97,000, 80,000, 72,000, ..
  30. ncbi Coordinate regulation of murein peptidase activity and AmpC beta-lactamase synthesis in Escherichia coli
    R E Bishop
    Department of Biochemistry, University of Alberta, Edmonton, Canada
    FEBS Lett 304:103-8. 1992
    In the periplasmic space of Escherichia coli, the (L)-m-A2pm-(D)-m-A2pm peptide, the lipoprotein, and the AmpC beta-lactamase are controlled by growth rate...
  31. ncbi Initiation of translation makes attenuation of ampC in E. coli dependent on growth rate
    T Grundstrom
    Mol Gen Genet 198:411-5. 1985
    The chromosomal beta-lactamase gene of E. coli, ampC, shows increased expression with increased growth rate of the bacteria...
  32. pmc Resistance of Escherichia coli to penicillins: identification of the structural gene for the chromosomal penicillinase
    L G Burman
    J Bacteriol 116:123-30. 1973
    ..is concluded that the mutations have occurred in the structural gene of the chromosomal penicillinase (designated ampC)...
  33. ncbi Insertion of IS2 creates a novel ampC promoter in Escherichia coli
    B Jaurin
    Cell 32:809-16. 1983
    A class of ampC beta-lactamase-hyperproducing mutants of Escherichia coli were shown to have the insertion element IS2 inserted into the ampC promoter...
  34. pmc Physical mapping and expression of hybrid plasmids carrying chromosomal beta-lactamase genes of Escherichia coli K-12
    T Grundstrom
    J Bacteriol 143:1127-34. 1980
    Hybrid plasmids carrying the ampC gene of Escherichia coli K-12 that codes for the chromosomal beta-lactamase were physically studied. The ampC gene was mapped to a deoxyribonucleic acid segment encompassing 1,370 base pairs...
  35. pmc Sequence elements determining ampC promoter strength in E. coli
    B Jaurin
    EMBO J 1:875-81. 1982
    A number of spontaneous up-promoter mutations have been isolated in the ampC beta-lactamase gene of Escherichia coli. The mutants were analyzed by DNA sequencing, and the level of ampC gene expression was determined...
  36. pmc ampC cephalosporinase of Escherichia coli K-12 has a different evolutionary origin from that of beta-lactamases of the penicillinase type
    B Jaurin
    Proc Natl Acad Sci U S A 78:4897-901. 1981
    A 1536-nucleotide-long sequence that carries the ampC beta-lactamase gene of the Escherichia coli K-12 chromosome has been determined...
  37. pmc The complexed structure and antimicrobial activity of a non-beta-lactam inhibitor of AmpC beta-lactamase
    R A Powers
    Department of Molecular Pharmacology and Biological Chemistry, Northwestern University Medical School, Chicago, Illinois 60611 3008, USA
    Protein Sci 8:2330-7. 1999
    ..resistance, non-beta-lactam inhibitors of beta-lactamases were investigated for complementarity to the structure of AmpC beta-lactamase from Escherichia coli...
  38. ncbi Recombination between short DNA homologies causes tandem duplication
    T Edlund
    Nature 292:269-71. 1981
    The ampC gene of Escherichia coli K-12 codes for a beta-lactamase which can hydrolyse the beta-lactam ring of ampicillin...
  39. ncbi Complementation of growth defect in an ampC deletion mutant of Escherichia coli
    R E Bishop
    Department of Biochemistry, University of Alberta, Edmonton, Canada
    FEMS Microbiol Lett 114:349-54. 1993
    beta-Lactamase genes of class-A (Rtem) and class-C (ampC) were placed under control of an inducible tac-promoter and expressed in Escherichia coli. Expression of RTEM had no observable effect on the growth properties of E...
  40. pmc Escherichia coli mutants lacking all possible combinations of eight penicillin binding proteins: viability, characteristics, and implications for peptidoglycan synthesis
    S A Denome
    Department of Microbiology and Immunology, School of Medicine, University of North Dakota, Grand Forks, North Dakota 58202 9037, USA
    J Bacteriol 181:3981-93. 1999
    ..The genes encoding PBPs 1a, 1b, 4, 5, 6, and 7, AmpC, and AmpH were cloned, and from each gene an internal coding sequence was removed and replaced with a kanamycin ..
  41. ncbi Three-dimensional structure of AmpC beta-lactamase from Escherichia coli bound to a transition-state analogue: possible implications for the oxyanion hypothesis and for inhibitor design
    K C Usher
    Institute of Molecular Biology, University of Oregon, Eugene 97403, USA
    Biochemistry 37:16082-92. 1998
    The structures of AmpC beta-lactamase from Escherichia coli, alone and in complex with a transition-state analogue, have been determined by X-ray crystallography. The native enzyme was determined to 2...
  42. pmc AmpC and AmpH, proteins related to the class C beta-lactamases, bind penicillin and contribute to the normal morphology of Escherichia coli
    T A Henderson
    Department of Microbiology and Immunology, School of Medicine, University of North Dakota, Grand Forks 58202 9037, USA
    J Bacteriol 179:6112-21. 1997
    ..The genes encoding these proteins were subcloned, sequenced, and identified. One gene was ampC, which encodes a chromosomal class C beta-lactamase. The second gene was located at about 8.5 min on the E...
  43. pmc Penicillin-binding proteins and induction of AmpC beta-lactamase
    C C Sanders
    Center for Research in Anti Infectives and Biotechnology, Department of Medical Microbiology, Creighton University School of Medicine, Omaha, Nebraska 68178, USA
    Antimicrob Agents Chemother 41:2013-5. 1997
    ..penicillin, penicillin-binding proteins (PBPs) 4, 7a, and 7b showed very high affinities for strong inducers of AmpC beta-lactamase. Loss of PBP 4 resulted in diminished inducibility...
  44. ncbi Stationary phase expression of a novel Escherichia coli outer membrane lipoprotein and its relationship with mammalian apolipoprotein D. Implications for the origin of lipocalins
    R E Bishop
    Medical Research Council Group in the Molecular Biology of Membranes, University of Alberta, Edmonton, Canada
    J Biol Chem 270:23097-103. 1995
    We report a novel outer membrane lipoprotein of Escherichia coli. DNA sequencing between ampC and sugE at the 94.5 min region of the E. coli chromosome revealed an open reading frame specifying 177 amino acid residues...
  45. pmc Overlap between ampC and frd operons on the Escherichia coli chromosome
    T Grundstrom
    Proc Natl Acad Sci U S A 79:1111-5. 1982
    The promoter for the Escherichia coli ampC beta-lactamase gene is shown to be located within the last gene of the fumarate reductase (frd) operon...
  46. pmc ampC beta-lactamase hyperproduction in Escherichia coli: natural ampicillin resistance generated by horizontal chromosomal DNA transfer from Shigella
    O Olsson
    Proc Natl Acad Sci U S A 80:7556-60. 1983
    Six ampicillin-resistant clinical isolates of Escherichia coli that hyperproduced the chromosomal ampC beta-lactamase were studied...
  47. pmc Inhibitor-sensitive AmpC beta-lactamase variant produced by an Escherichia coli clinical isolate resistant to oxyiminocephalosporins and cephamycins
    Yohei Doi
    Department of Bacterial Pathogenesis and Infection Control, National Institute of Infectious Diseases, 4 7 1 Gakuen, Musashi Murayama, Tokyo 208 0011, Japan
    Antimicrob Agents Chemother 48:2652-8. 2004
    ..coli HKY28, a ceftazidime-resistant strain isolated from a urine specimen in Japan, produced an inhibitor-sensitive AmpC beta-lactamase variant...
  48. ncbi Nucleotide sequence coding for the flavoprotein subunit of the fumarate reductase of Escherichia coli
    S T Cole
    Eur J Biochem 122:479-84. 1982
    ..This sequence contains a histidyl residue which probably services as the site for attachment of the FAD cofactor to the reductase...
  49. pmc AmpC beta-lactamase in an Escherichia coli clinical isolate confers resistance to expanded-spectrum cephalosporins
    Hedi Mammeri
    Service de Bacteriologie Virologie, Hopital de Bicetre, 78 rue du General Leclerc, 94275 Le Kremlin Bicetre cedex, France
    Antimicrob Agents Chemother 48:4050-3. 2004
    Cloning, sequencing, and biochemical analysis identified a novel AmpC-type beta-lactamase conferring resistance to extended-spectrum cephalosporins in an Escherichia coli clinical isolate...
  50. ncbi Nanomolar inhibitors of AmpC beta-lactamase
    Federica Morandi
    Department of Pharmaceutical Chemistry, University of California, San Francisco, Mission Bay Genentech Hall, 600 16th Street, Mail Box 2240, 94143, USA
    J Am Chem Soc 125:685-95. 2003
    ..evaluate new carboxyphenyl-glycylboronic acid transition-state analogue inhibitors of the class C beta-lactamase AmpC. The new compounds improve inhibition by over 2 orders of magnitude compared to analogous glycylboronic acids, with ..
  51. ncbi Cloning and sequencing of the beta-lactamase gene and surrounding DNA sequences of Citrobacter braakii, Citrobacter murliniae, Citrobacter werkmanii, Escherichia fergusonii and Enterobacter cancerogenus
    Thierry Naas
    Service de Bacteriologie Virologie, Hopital de Bicetre, Assistance Publique Hopitaux de Paris, Faculte de Medecine Paris Sud, 78 rue du General Leclerc, 94275 Le Kremlin Bicetre cedex, France
    FEMS Microbiol Lett 215:81-7. 2002
    ..These beta-lactamases had all a single pI value >8 and conferred a typical AmpC-type resistance pattern in E. coli recombinant strains...
  52. pmc Naturally occurring extended-spectrum cephalosporinases in Escherichia coli
    Hedi Mammeri
    Service de Bacteriologie Virologie, Hopital de Bicetre, Assistance Publique Hopitaux de Paris, Faculte de Medecine Paris Sud, Universite Paris Sud, 94275 Le Kremlin Bicetre, France
    Antimicrob Agents Chemother 50:2573-6. 2006
    ..produced by 65 clonally unrelated clinical Escherichia coli isolates revealed genetic diversity of the ampC genes and showed that Gln287, Cys287, Pro296, Leu298, and Phe350 substitutions were involved in extension of the ..
  53. ncbi Structural bases of stability-function tradeoffs in enzymes
    Beth M Beadle
    Department of Molecular Pharmacology and Biological Chemistry, Northwestern University School of Medicine, 303 East Chicago Avenue S215, Chicago, IL 60611 3008, USA
    J Mol Biol 321:285-96. 2002
    ..Using AmpC beta-lactamase, an enzyme that is well-characterized structurally and mechanistically, the relationship between ..
  54. ncbi Structures of ceftazidime and its transition-state analogue in complex with AmpC beta-lactamase: implications for resistance mutations and inhibitor design
    R A Powers
    Department of Molecular Pharmacology and Biological Chemistry, Northwestern University, 303 East Chicago Avenue, Chicago, Illinois 60611, USA
    Biochemistry 40:9207-14. 2001
    ..class C beta-lactamases and how mutant enzymes might overcome this, the structures of the class C beta-lactamase AmpC in complex with the third-generation cephalosporin ceftazidime and with a transition-state analogue of ceftazidime ..
  55. pmc Large-scale identification of protein-protein interaction of Escherichia coli K-12
    Mohammad Arifuzzaman
    Graduate School of Biological Sciences, Nara Institute of Science and Technology, Ikoma, Nara 630 0101, Japan
    Genome Res 16:686-91. 2006
    ..An extended analysis of these interacting networks by bioinformatics and experimentation should provide new insights and novel strategies for E. coli systems biology...

Research Grants1

  1. Plasmid-Mediated Quinolone Resistance
    George Jacoby; Fiscal Year: 2007
    ..unreadable] [unreadable]..