GP

Summary

Gene Symbol: GP
Description: virion spike glycoprotein precursor
Alias: sGP, ssGP
Species: Ebola virus - Mayinga, Zaire, 1976

Top Publications

  1. pmc Ebola virus can be effectively neutralized by antibody produced in natural human infection
    T Maruyama
    Departments of Immunology and Molecular Biology, The Scripps Research Institute, La Jolla, California 92037, USA
    J Virol 73:6024-30. 1999
  2. ncbi Epitopes involved in antibody-mediated protection from Ebola virus
    J A Wilson
    Virology Division, U S Army Medical Research Institute of Infectious Diseases, 1425 Porter Street, Fort Detrick, Frederick, MD 21702 5011, USA
    Science 287:1664-6. 2000
  3. ncbi The assembly of Ebola virus nucleocapsid requires virion-associated proteins 35 and 24 and posttranslational modification of nucleoprotein
    Yue Huang
    Vaccine Research Center, National Institutes of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Mol Cell 10:307-16. 2002
  4. pmc In vivo oligomerization and raft localization of Ebola virus protein VP40 during vesicular budding
    Rekha G Panchal
    Developmental Therapeutics Program, Target Structure Based Drug Discovery Group, Science Applications International Corporation, National Cancer Institute, Frederick, MD 21702 1201, USA
    Proc Natl Acad Sci U S A 100:15936-41. 2003
  5. pmc Lipid raft microdomains: a gateway for compartmentalized trafficking of Ebola and Marburg viruses
    Sina Bavari
    Dept of Cell Biology and Biochemistry, U S Army Medical Research Institute of Infectious Diseases, Frederick, MD 21702 5011, USA
    J Exp Med 195:593-602. 2002
  6. pmc Core structure of the envelope glycoprotein GP2 from Ebola virus at 1.9-A resolution
    V N Malashkevich
    Howard Hughes Medical Institute, Whitehead Institute for Biomedical Research, Department of Biology, Massachusetts Institute of Technology, Nine Cambridge Center, Cambridge, MA 02142, USA
    Proc Natl Acad Sci U S A 96:2662-7. 1999
  7. pmc Cathepsin cleavage potentiates the Ebola virus glycoprotein to undergo a subsequent fusion-relevant conformational change
    Matthew Brecher
    Department of Microbiology, University of Virginia, Charlottesville, Virginia, USA
    J Virol 86:364-72. 2012
  8. ncbi [Research progress on ebola virus glycoprotein]
    Guo Yong Ding
    School of Public Health, Taishan Medical College, Taian 271016, China
    Bing Du Xue Bao 29:233-7. 2013
  9. pmc A mutation in the Ebola virus envelope glycoprotein restricts viral entry in a host species- and cell-type-specific manner
    Osvaldo Martinez
    Icahn School of Medicine at Mount Sinai, New York, NY, USA
    J Virol 87:3324-34. 2013
  10. pmc Ebola virus entry requires the host-programmed recognition of an intracellular receptor
    Emily Happy Miller
    Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, NY, USA
    EMBO J 31:1947-60. 2012

Scientific Experts

  • Reed F Johnson
  • Anthony Sanchez
  • Sina Bavari
  • Kelly L Warfield
  • Heinz Feldmann
  • Andreas Lucht
  • Alexander A Bukreyev
  • Ayato Takada
  • Yoshihiro Kawaoka
  • Kartik Chandran
  • Darryl Falzarano
  • Gabriele Neumann
  • Jochen Seebach
  • Hans Joachim Schnittler
  • Emily Happy Miller
  • E L Subbotina
  • Nathalie Alazard-Dany
  • Sean P Whelan
  • V E Volchkov
  • V A Volchkova
  • Dipankar Das
  • Thomas W Geisbert
  • Nancy J Sullivan
  • Osvaldo Martinez
  • Guo Yong Ding
  • Viktor E Volchkov
  • Esther Ndungo
  • Matthew Brecher
  • Daniel Olal
  • Jillian M Licata
  • John M Dye
  • Shinji Watanabe
  • Erica Ollmann Saphire
  • Marnie L Fusco
  • Ana I Kuehne
  • M Javad Aman
  • Judith M White
  • Sue E Delos
  • H D Klenk
  • Sonia M Gregory
  • Rohini G Sandesara
  • Masfique Mehedi
  • Melinda A Brindley
  • Anthony C Wong
  • Lisa A Lopez
  • Joseph R Francica
  • Frederic Baribaud
  • Gordon Ruthel
  • Wendelien B Oswald
  • Joan B Geisbert
  • Soraya Shahhosseini
  • Mavanur R Suresh
  • Steven M Jones
  • Monica S Freitas
  • Hideki Ebihara
  • Oleg Krokhin
  • A A Chepurnov
  • Kristin Wolf
  • Peter B Jahrling
  • Josh D Silvertown
  • Jonathan S Towner
  • Julie E Martin
  • Jens H Kuhn
  • Bettina Hartlieb
  • Xiangguo Qiu
  • Stuart T Nichol
  • Ling Ye
  • Paul Bates
  • Sarah E McCarthy
  • Séverine Bär
  • Kavitha Yaddanapudi
  • Masayuki Shimojima
  • Steven Jones
  • Olivier Reynard
  • Viktor Volchkov
  • Olga Dolnik
  • Winfried Weissenhorn
  • L J Earp
  • Ronald N Harty
  • Chris Druar
  • Victoria M Wahl-Jensen
  • Balaji Manicassamy
  • Thomas Hoenen
  • Dana L Swenson
  • Luke D Jasenosky
  • Gary J Nabel
  • E G Nabel
  • Graham Simmons
  • G J Nabel
  • Rekha G Panchal

Detail Information

Publications74

  1. pmc Ebola virus can be effectively neutralized by antibody produced in natural human infection
    T Maruyama
    Departments of Immunology and Molecular Biology, The Scripps Research Institute, La Jolla, California 92037, USA
    J Virol 73:6024-30. 1999
    ..Antibodies reactive with nucleoprotein (NP), envelope glycoprotein (GP), and secreted envelope glycoprotein (sGP) were characterized by immunofluorescence and radioimmunoprecipitation assays...
  2. ncbi Epitopes involved in antibody-mediated protection from Ebola virus
    J A Wilson
    Virology Division, U S Army Medical Research Institute of Infectious Diseases, 1425 Porter Street, Fort Detrick, Frederick, MD 21702 5011, USA
    Science 287:1664-6. 2000
    ..The identification of protective mAbs has important implications for developing vaccines and therapies for Ebola virus...
  3. ncbi The assembly of Ebola virus nucleocapsid requires virion-associated proteins 35 and 24 and posttranslational modification of nucleoprotein
    Yue Huang
    Vaccine Research Center, National Institutes of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Mol Cell 10:307-16. 2002
    ..This distinct mechanism of regulation for filovirus assembly suggests new approaches for viral therapies and vaccines for Ebola and related viruses...
  4. pmc In vivo oligomerization and raft localization of Ebola virus protein VP40 during vesicular budding
    Rekha G Panchal
    Developmental Therapeutics Program, Target Structure Based Drug Discovery Group, Science Applications International Corporation, National Cancer Institute, Frederick, MD 21702 1201, USA
    Proc Natl Acad Sci U S A 100:15936-41. 2003
    ..We also report the successful application of the biarsenic fluorophore, FlAsH, combined with a tetracysteine tag for imaging of Ebola VP40 in live cells...
  5. pmc Lipid raft microdomains: a gateway for compartmentalized trafficking of Ebola and Marburg viruses
    Sina Bavari
    Dept of Cell Biology and Biochemistry, U S Army Medical Research Institute of Infectious Diseases, Frederick, MD 21702 5011, USA
    J Exp Med 195:593-602. 2002
    ....
  6. pmc Core structure of the envelope glycoprotein GP2 from Ebola virus at 1.9-A resolution
    V N Malashkevich
    Howard Hughes Medical Institute, Whitehead Institute for Biomedical Research, Department of Biology, Massachusetts Institute of Technology, Nine Cambridge Center, Cambridge, MA 02142, USA
    Proc Natl Acad Sci U S A 96:2662-7. 1999
    Ebola virions contain a surface transmembrane glycoprotein (GP) that is responsible for binding to target cells and subsequent fusion of the viral and host-cell membranes...
  7. pmc Cathepsin cleavage potentiates the Ebola virus glycoprotein to undergo a subsequent fusion-relevant conformational change
    Matthew Brecher
    Department of Microbiology, University of Virginia, Charlottesville, Virginia, USA
    J Virol 86:364-72. 2012
    Cellular entry of Ebola virus (EBOV), a deadly hemorrhagic fever virus, is mediated by the viral glycoprotein (GP). The receptor-binding subunit of GP must be cleaved (by endosomal cathepsins) in order for entry and infection to proceed...
  8. ncbi [Research progress on ebola virus glycoprotein]
    Guo Yong Ding
    School of Public Health, Taishan Medical College, Taian 271016, China
    Bing Du Xue Bao 29:233-7. 2013
    ..The glycoprotein (GP) of EBOV is a transmembrane envelope protein known to play multiple functions including virus attachment and entry, ..
  9. pmc A mutation in the Ebola virus envelope glycoprotein restricts viral entry in a host species- and cell-type-specific manner
    Osvaldo Martinez
    Icahn School of Medicine at Mount Sinai, New York, NY, USA
    J Virol 87:3324-34. 2013
    ..A single viral glycoprotein (GP) mediates viral attachment and entry...
  10. pmc Ebola virus entry requires the host-programmed recognition of an intracellular receptor
    Emily Happy Miller
    Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, NY, USA
    EMBO J 31:1947-60. 2012
    ..The second luminal domain of NPC1 binds directly and specifically to the viral glycoprotein, GP, and a synthetic single-pass membrane protein containing this domain has viral receptor activity...
  11. pmc Structure of an antibody in complex with its mucin domain linear epitope that is protective against Ebola virus
    Daniel Olal
    Department of Immunology and Microbial Science, The Scripps Research Institute, La Jolla, California, USA
    J Virol 86:2809-16. 2012
    ..and recognizes a distinct linear epitope in the prominent mucin-like domain of the Ebola virus glycoprotein GP. The structure of 14G7 in complex with its linear peptide epitope has now been determined to 2.8 Å...
  12. pmc Contribution of ebola virus glycoprotein, nucleoprotein, and VP24 to budding of VP40 virus-like particles
    Jillian M Licata
    Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, 3800 Spruce St, Philadelphia, PA 19104 6049, USA
    J Virol 78:7344-51. 2004
    ..utilized a functional budding assay and cotransfection experiments to examine the contributions of the glycoprotein (GP), nucleoprotein (NP), and VP24 of Ebola virus in facilitating release of VP40 VLPs...
  13. ncbi Ebola virus glycoprotein GP is not cytotoxic when expressed constitutively at a moderate level
    Nathalie Alazard-Dany
    Filovirus Laboratory, Claude Bernard University Lyon 1, INSERM U758, IFR 128 Biosciences Lyon Gerland, France
    J Gen Virol 87:1247-57. 2006
    Transient expression of Ebola virus (EBOV) glycoprotein GP causes downregulation of surface proteins, cell rounding and detachment, a phenomenon believed to play a central role in the pathogenicity of the virus...
  14. ncbi Polymorphism of filovirus glycoproteins
    Viktor E Volchkov
    Biologie des Filovirus, Claude Bernard University Lyon, INSERM U412 69365 Lyon, France
    Adv Virus Res 64:359-81. 2005
  15. ncbi Analysis of the expressed heavy chain variable-region genes of Macaca fascicularis and isolation of monoclonal antibodies specific for the Ebola virus' soluble glycoprotein
    Chris Druar
    Cangene Corporation, 3404 American Drive, Mississauga, Ontario L4V 1T4, Canada
    Immunogenetics 57:730-8. 2005
    ..In summary, the study provides new insight into the M. fascicularis V region gene repertoire and further supports the idea that macaque-derived mAbs may be of therapeutic value to humans...
  16. pmc A DNA vaccine for Ebola virus is safe and immunogenic in a phase I clinical trial
    Julie E Martin
    Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes, Bethesda, MD 20892 3017, USA
    Clin Vaccine Immunol 13:1267-77. 2006
    ..A three-plasmid DNA vaccine encoding the envelope glycoproteins (GP) from the Zaire and Sudan/Gulu species as well as the nucleoprotein was evaluated in a randomized, placebo-..
  17. ncbi Structure-function analysis of the soluble glycoprotein, sGP, of Ebola virus
    Darryl Falzarano
    Department of Medical Microbiology, University of Manitoba Winnipeg, Manitoba R3E 0W3, Canada
    Chembiochem 7:1605-11. 2006
    ..addition to the transmembrane protein, GP(1,2), the Ebola virus glycoprotein gene encodes the soluble glycoproteins sGP and Delta-peptide...
  18. ncbi A luciferase-based budding assay for Ebola virus
    Sarah E McCarthy
    Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, 3800 Spruce St, Philadelphia, PA 19104, USA
    J Virol Methods 137:115-9. 2006
    ..Luciferase was found to be incorporated into budding VP40 VLPs. Furthermore, co-expression of EBOV glycoprotein (GP) enhances release of VLPs containing VP40 and luciferase...
  19. ncbi Functional expression of mouse relaxin and mouse relaxin-3 in the lung from an Ebola virus glycoprotein-pseudotyped lentivirus via tracheal delivery
    Josh D Silvertown
    Division of Stem Cell and Developmental Biology, Ontario Cancer Institute, University Health Network, Toronto, Ontario, Canada M5G 2M1
    Endocrinology 147:3797-808. 2006
    ..with enhanced tropism for the lung, LVs were pseudotyped with the Zaire strain of the Ebola virus glycoprotein (EboZ GP) and delivered by endotracheal intubation...
  20. ncbi Filovirus assembly and budding
    Bettina Hartlieb
    Institut fur Virologie, Robert Koch str 17, 35037 Marburg, Germany
    Virology 344:64-70. 2006
    ..As a consequence, assembly and budding steps are defined to specific cellular compartments, and the recent progress in understanding how the different components are assembled into stable enveloped virus particles is reviewed...
  21. ncbi Ebola virus-like particles produced in insect cells exhibit dendritic cell stimulating activity and induce neutralizing antibodies
    Ling Ye
    Department of Microbiology and Immunology and Emory Vaccine Center, Emory University School of Medicine, Atlanta, GA 30322, USA
    Virology 351:260-70. 2006
    Recombinant baculoviruses (rBV) expressing Ebola virus VP40 (rBV-VP40) or GP (rBV-GP) proteins were generated...
  22. pmc Detection of cell-cell fusion mediated by Ebola virus glycoproteins
    Séverine Bär
    Department of Cell Biology, Institut Cochin, INSERM U567, CNRS UMR 8104, Universite Rene Descartes, Paris, France
    J Virol 80:2815-22. 2006
    ..viruses (EboV) are enveloped RNA viruses infecting cells by a pH-dependent process mediated by viral glycoproteins (GP) involving endocytosis of virions and their routing into acidic endosomes...
  23. ncbi [Ebola and Marburg viruses: the humans strike back]
    Nathalie Alazard-Dany
    Laboratoire des Filovirus, INSERM U758, ENS Lyon, IFR 128 Biosciences Lyon Gerland, Universite Claude Bernard Lyon 1, France
    Med Sci (Paris) 22:405-10. 2006
    ..Chandran et al. have elucidated the mechanism by which Ebola GP is converted to a fusion-active form...
  24. ncbi [The properties of Ebola virus proteins]
    E L Subbotina
    Vopr Virusol 51:4-10. 2006
    ..presents information on the role of structural (NP, VP40, VP35, GP, VP30, VP24, and L) and secreted (sGP, delta-peptide, GP1, GP(1,2delta), ssGP) proteins in the viral replication cycle and in the pathogenesis of Ebola hemorrhagic fever.
  25. pmc Tyro3 family-mediated cell entry of Ebola and Marburg viruses
    Masayuki Shimojima
    Division of Virology, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, 4 6 1 Shirokanedai, Minato ku, Tokyo 108 8639, Japan
    J Virol 80:10109-16. 2006
    ..Antibody to Axl inhibited infection of pseudotype viruses in a number of Axl-positive cell lines. These results implicate each Tyro3 family member as a cell entry factor in filovirus infection...
  26. pmc Proteolytic processing of the Ebola virus glycoprotein is not critical for Ebola virus replication in nonhuman primates
    Gabriele Neumann
    Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin Madison, 2015 Linden Drive, Madison, WI 53706, USA
    J Virol 81:2995-8. 2007
    ..We conclude that glycoprotein cleavage by subtilisin-like endoproteases is not critical for Ebola virus infectivity and virulence in nonhuman primates...
  27. pmc Structure and function of the complete internal fusion loop from Ebolavirus glycoprotein 2
    Sonia M Gregory
    Center for Membrane Biology, University of Virginia, Charlottesville, VA 22908, USA
    Proc Natl Acad Sci U S A 108:11211-6. 2011
    ..The viral glycoprotein (GP) is solely responsible for virus-host membrane fusion, but how it does so remains elusive...
  28. pmc Tyrosine kinase receptor Axl enhances entry of Zaire ebolavirus without direct interactions with the viral glycoprotein
    Melinda A Brindley
    Department of Microbiology, University of Iowa, Iowa City, IA 52242, USA
    Virology 415:83-94. 2011
    ..Using one of these cell lines, we identified ZEBOV entry events that are Axl-dependent. Interactions between ZEBOV-GP and the Axl ectodomain were not detected in immunoprecipitations and reduction of surface-expressed Axl by RNAi did ..
  29. pmc A new Ebola virus nonstructural glycoprotein expressed through RNA editing
    Masfique Mehedi
    Department of Medical Microbiology, University of Manitoba, Public Health Agency of Canada, Winnipeg, Manitoba, Canada
    J Virol 85:5406-14. 2011
    ..A third GP gene product, a small soluble glycoprotein (ssGP), has long been postulated to be produced also as a result of transcriptional editing...
  30. pmc Ebola virus glycoprotein counteracts BST-2/Tetherin restriction in a sequence-independent manner that does not require tetherin surface removal
    Lisa A Lopez
    Department of Molecular Microbiology and Immunology, USC Keck School of Medicine, 2011 Zonal Ave, HMR502, Los Angeles, CA 90033, USA
    J Virol 84:7243-55. 2010
    ..Strikingly, the anti-tetherin factor, Ebola virus GP, was able to overcome restriction conferred by both tetherin and a series of functional tetherin derivatives, ..
  31. pmc A forward genetic strategy reveals destabilizing mutations in the Ebolavirus glycoprotein that alter its protease dependence during cell entry
    Anthony C Wong
    Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    J Virol 84:163-75. 2010
    Ebolavirus (EBOV) entry into cells requires proteolytic disassembly of the viral glycoprotein, GP. This proteolytic processing, unusually extensive for an enveloped virus entry protein, is mediated by cysteine cathepsins, a family of ..
  32. pmc Requirements for cell rounding and surface protein down-regulation by Ebola virus glycoprotein
    Joseph R Francica
    Department of Microbiology, University of Pennsylvania School of Medicine, Philadelphia, 19104 6076, USA
    Virology 383:237-47. 2009
    ..Cellular pathogenesis can be modeled in vitro by expression of the Ebola viral glycoprotein (GP) in cells, which causes dramatic morphological changes, including cell rounding and surface protein down-regulation...
  33. ncbi Ebola sGP--the first viral glycoprotein shown to be C-mannosylated
    Darryl Falzarano
    Department of Medical Microbiology, University of Manitoba, Winnipeg, Manitoba, Canada R3E 0W3
    Virology 368:83-90. 2007
    Mass spectrometry analysis of the Ebola virus soluble glycoprotein sGP identified a rare post-translation modification, C-mannosylation, which was found on tryptophan (W) 288...
  34. ncbi Structure of the Ebola fusion peptide in a membrane-mimetic environment and the interaction with lipid rafts
    Monica S Freitas
    Programa de Biologia Estrutural, Instituto de Bioquimica Medica, Centro Nacional de Ressonancia Magnetica Nuclear Jiri Jonas, Universidade Federal do Rio de Janeiro, 21941 590 Rio de Janeiro, RJ, Brazil
    J Biol Chem 282:27306-14. 2007
    ..Our data shed light on the structural "domains" of fusion peptides and provide a clue for the development of a drug that might block the early steps of viral infection...
  35. ncbi Differential expression of the Ebola virus GP(1,2) protein and its fragments in E. coli
    Dipankar Das
    University of Alberta, Edmonton, Alta, Canada T6G 2N8
    Protein Expr Purif 54:117-25. 2007
    ..The full length Ebola virus (EBOV) GP(1,2) gene and subfragments of the GP(1) gene were cloned in a bacterial expression vector as a C-terminal His(6) ..
  36. ncbi Production and characterization of monoclonal antibodies against different epitopes of Ebola virus antigens
    Soraya Shahhosseini
    Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, Edmonton, AB, Canada
    J Virol Methods 143:29-37. 2007
    ..MAbs of different epitope specificities against EBOV antigens [virion protein (VP40), secreted glycoprotein (sGP), and GP(1) subfragment D] were developed...
  37. ncbi [Molecular mechanisms of Ebola virus reproduction]
    E L Subbotina
    Vopr Virusol 52:10-6. 2007
    ....
  38. pmc Neutralizing antibody fails to impact the course of Ebola virus infection in monkeys
    Wendelien B Oswald
    Departments of Immunology and Molecular Biology, The Scripps Research Institute, La Jolla, California, United States of America
    PLoS Pathog 3:e9. 2007
    ..It appears that Ebola virus has a mechanism of infection propagation in vivo in macaques that is uniquely insensitive even to high concentrations of neutralizing antibody...
  39. pmc Effects of Ebola virus glycoproteins on endothelial cell activation and barrier function
    Victoria M Wahl-Jensen
    Special Pathogens Program, National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba
    J Virol 79:10442-50. 2005
    ..In contrast, the soluble glycoproteins sGP and delta-peptide did not activate endothelial cells or change the endothelial barrier function...
  40. ncbi Filovirus-like particles as vaccines and discovery tools
    Kelly L Warfield
    United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, MD 21702 5011, USA
    Expert Rev Vaccines 4:429-40. 2005
    ..Thus, in addition to their use as a vaccine, VLPs are currently being used as tools to learn lessons about filovirus pathogenesis, immunology, replication and assembly requirements...
  41. ncbi Recovery of infectious Ebola virus from complementary DNA: RNA editing of the GP gene and viral cytotoxicity
    V E Volchkov
    Institut fur Virologie, Philipps Universitat, Robert Koch Strasse 17, 35037 Marburg, Germany
    Science 291:1965-9. 2001
    ..This mutant no longer expressed the nonstructural glycoprotein sGP. Synthesis of GP increased, but most of it accumulated in the endoplasmic reticulum as immature precursor...
  42. ncbi Identification of the Ebola virus glycoprotein as the main viral determinant of vascular cell cytotoxicity and injury
    Z Y Yang
    Vaccine Research Center, National Institutes of Health, 40 Convent Drive, Bethesda, Maryland 20892 3005, USA
    Nat Med 6:886-9. 2000
    Here we defined the main viral determinant of Ebola virus pathogenicity; synthesis of the virion glycoprotein (GP) of Ebola virus Zaire induced cytotoxic effects in human endothelial cells in vitro and in vivo...
  43. ncbi Delta-peptide is the carboxy-terminal cleavage fragment of the nonstructural small glycoprotein sGP of Ebola virus
    V A Volchkova
    Institut fur Virologie, Philipps Universitat Marburg, Marburg, 35011, Germany
    Virology 265:164-71. 1999
    In the present study we have investigated processing and maturation of the nonstructural small glycoprotein (sGP) of Ebola virus...
  44. ncbi Classification, structure, and replication of filoviruses
    H Feldmann
    Institut fur Virologie, Philipps Universitat, Marburg, Germany
    Curr Top Microbiol Immunol 235:1-21. 1999
  45. pmc Endoproteolytic processing of the ebola virus envelope glycoprotein: cleavage is not required for function
    R J Wool-Lewis
    Department of Microbiology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104 6076, USA
    J Virol 73:1419-26. 1999
    ..Here we show that the envelope glycoprotein from the Zaire strain of Ebola virus (Ebo-GP) is proteolytically processed into two subunits, GP1 and GP2, that are likely covalently associated through a ..
  46. ncbi The nonstructural small glycoprotein sGP of Ebola virus is secreted as an antiparallel-orientated homodimer
    V A Volchkova
    Institut fur Virologie, Philipps Universitat, Robert Koch Strasse 17, Marburg, D 35037, Germany
    Virology 250:408-14. 1998
    ..Another editing product of the GP gene of Ebola virus (ssGP), which shares 295 amino-terminal amino acid residues with sGP, is secreted from cells in a monomeric form due to ..
  47. pmc Biochemical analysis of the secreted and virion glycoproteins of Ebola virus
    A Sanchez
    Special Pathogens Branch, Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA
    J Virol 72:6442-7. 1998
    ..The 50- to 70-kDa secreted and 150-kDa virion/structural glycoproteins (SGP and GP, respectively), which share the 295 N-terminal residues, are cleaved near the N terminus by signalase...
  48. ncbi Release of viral glycoproteins during Ebola virus infection
    V E Volchkov
    Institut fur Virologie, Philipps Universitat Marburg, Germany
    Virology 245:110-9. 1998
    Maturation and release of the Ebola virus glycoprotein GP were studied in cells infected with either Ebola or recombinant vaccinia viruses. Significant amounts of GP were found in the culture medium in nonvirion forms...
  49. ncbi Distinct cellular interactions of secreted and transmembrane Ebola virus glycoproteins
    Z Yang
    Howard Hughes Medical Institute and Department of Internal Medicine, University of Michigan, Ann Arbor, MI 48109, USA
    Science 279:1034-7. 1998
    ....
  50. ncbi Immunization for Ebola virus infection
    L Xu
    Department of Biological Chemistry, University of Michigan Medical Center, Ann Arbor 48109 0650, USA
    Nat Med 4:37-42. 1998
    ..immune responses to the viral nucleoprotein (NP) and the secreted or transmembrane forms of the glycoprotein (sGP or GP) and their ability to protect against infection in a guinea pig infection model analogous to the human ..
  51. ncbi Ebola takes a punch
    T Folks
    Nat Med 4:16-7. 1998
  52. pmc A system for functional analysis of Ebola virus glycoprotein
    A Takada
    Department of Virology and Molecular Biology, St Jude Children s Research Hospital, 332 North Lauderdale, P O Box 318, Memphis, TN 38101, USA
    Proc Natl Acad Sci U S A 94:14764-9. 1997
    ..Thus, our VSV system should be useful for investigating the functions of glycoproteins from highly pathogenic viruses or those incapable of being cultured in vitro...
  53. ncbi Termini of all mRNA species of Marburg virus: sequence and secondary structure
    E Muhlberger
    Institut fur Virologie, Philipps Universitat Marburg, Germany
    Virology 223:376-80. 1996
    ....
  54. pmc The virion glycoproteins of Ebola viruses are encoded in two reading frames and are expressed through transcriptional editing
    A Sanchez
    Division of Viral and Ricketsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA
    Proc Natl Acad Sci U S A 93:3602-7. 1996
    ..This genetic stability may indicate that EBO viruses have coevolved with their natural reservoirs and do not change appreciably in the wild...
  55. ncbi [Josamycin in the treatment of impetigo in childhood: comparison with erythromycin]
    E Rampini
    G Ital Chemioter 29:155-64. 1982
  56. ncbi The pathogenesis of Ebola hemorrhagic fever
    A Takada
    Division of Virology, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, Tokyo 108 8639, Japan
    Trends Microbiol 9:506-11. 2001
    ....
  57. ncbi Downregulation of beta1 integrins by Ebola virus glycoprotein: implication for virus entry
    A Takada
    Laboratory of Microbiology, Graduate School of Veterinary Medicine, Sapporo, 060 0818, Japan
    Virology 278:20-6. 2000
    Filoviruses, including Ebola virus, are cytotoxic. To investigate the role of the Ebola virus glycoprotein (GP) in this cytopathic effect, we transiently expressed the GP in human kidney 293T cells...
  58. ncbi Endosomal proteolysis of the Ebola virus glycoprotein is necessary for infection
    Kartik Chandran
    Department of Medicine, Brigham and Women s Hospital and Harvard Medical School, Boston, MA 02115, USA
    Science 308:1643-5. 2005
    ..infection of African green monkey kidney (Vero) cells by vesicular stomatitis viruses bearing the EboV glycoprotein (GP) requires the activity of endosomal cysteine proteases...
  59. pmc Comprehensive analysis of ebola virus GP1 in viral entry
    Balaji Manicassamy
    Department of Microbiology and Immunology, College of Medicine, University of Illinois at Chicago, E829 MSB, 835 S Wolcott Ave, Chicago, IL 60612, USA
    J Virol 79:4793-805. 2005
    ..incorporation, suggesting that these residues are involved in the proper folding and/or overall conformation of GP. Sequence comparison of the GP1 proteins suggests that the majority of the critical residues for GP folding and ..
  60. ncbi The many mechanisms of viral membrane fusion proteins
    L J Earp
    Department of Microbiology, University of Virginia, Charlottesville, VA, USA
    Curr Top Microbiol Immunol 285:25-66. 2005
    ..We also briefly discuss the therapeutic potential of fusion inhibitors in treating viral infections...
  61. ncbi Generation of Marburg virus-like particles by co-expression of glycoprotein and matrix protein
    Dana L Swenson
    U S Army Medical Research Institute of Infectious Diseases, 1425 Porter Street, Frederick, MD 21702, USA
    FEMS Immunol Med Microbiol 40:27-31. 2004
    ..Furthermore, VLPs may be an excellent vaccine candidate against Marburg infection...
  62. ncbi Production of monoclonal antibodies and development of an antigen capture ELISA directed against the envelope glycoprotein GP of Ebola virus
    Andreas Lucht
    Bundeswehr Institute of Microbiology, Neuherbergstrasse 11, 80937 Munich, Germany
    Med Microbiol Immunol 193:181-7. 2004
    ..Antibodies directed against the viral glycoprotein GP were characterized by ELISA, Western blot and immunofluorescence analyses...
  63. pmc Antibody-dependent enhancement of Ebola virus infection
    Ayato Takada
    Division of Virology, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, Tokyo 108 8639, Japan
    J Virol 77:7539-44. 2003
    ..Our results suggest a novel mechanism of antibody-dependent enhancement of Ebola virus infection, one that would account for the dire outcome of Ebola outbreaks in human populations...
  64. ncbi DC-SIGN and DC-SIGNR bind ebola glycoproteins and enhance infection of macrophages and endothelial cells
    Graham Simmons
    Department of Molecular Histopathology, University of Cambridge, Cambridge, CB2 1QP, United Kingdom
    Virology 305:115-23. 2003
    ....
  65. pmc Identification of protective epitopes on ebola virus glycoprotein at the single amino acid level by using recombinant vesicular stomatitis viruses
    Ayato Takada
    Division of Virology, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, Japan
    J Virol 77:1069-74. 2003
    ..These data indicate that neutralizing antibody cocktails for passive prophylaxis and therapy of Ebola hemorrhagic fever can reduce the possibility of the emergence of antigenic variants in infected individuals...
  66. pmc Covalent modifications of the ebola virus glycoprotein
    Scott A Jeffers
    Department of Biological Sciences, Purdue University, 1392 Lilly Hall, West Lafayette, IN 47907, USA
    J Virol 76:12463-72. 2002
    The role of covalent modifications of the Ebola virus glycoprotein (GP) and the significance of the sequence identity between filovirus and avian retrovirus GPs were investigated through biochemical and functional analyses of mutant GPs...
  67. ncbi The role of DC-SIGN and DC-SIGNR in HIV and Ebola virus infection: can potential therapeutics block virus transmission and dissemination?
    Frederic Baribaud
    Department of Microbiology, University of Pennsylvania, 225 Johnson Pavilion, 3610 Hamilton Walk, Philadelphia, PA 19104, USA
    Expert Opin Ther Targets 6:423-31. 2002
    ..This article reviews the interaction of DC-SIGN and DC-SIGNR with HIV and Ebola, discusses the mechanism of DC-SIGN-mediated viral transmission and examines how this process could be inhibited by potential therapeutics...
  68. ncbi Descriptive analysis of Ebola virus proteins
    L H Elliott
    Virology 147:169-76. 1985
    ..Seven virion proteins were described; an L (180K), GP (125K), NP (104K), VP40 (40K), VP35 (35K), VP30 (30K), and VP24 (24K)...
  69. pmc VP40 octamers are essential for Ebola virus replication
    Thomas Hoenen
    Institut fur Virologie, Robert Koch str 17, 35037 Marburg, Germany
    J Virol 79:1898-905. 2005
    ..In contrast, no recombinant virus expressing the VP40 Arg134-to-Ala mutation could be recovered. These results suggest that RNA binding of VP40 and therefore octamer formation are essential for the Ebola virus life cycle...
  70. pmc Functional mapping of the nucleoprotein of Ebola virus
    Shinji Watanabe
    Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin Madison, 2015 Linden Dr, Madison, Wisconsin 53706, USA
    J Virol 80:3743-51. 2006
    ..These data advance our understanding of the functional region(s) of Ebola virus NP, which in turn should improve our knowledge of the Ebola virus life cycle and its extreme pathogenicity...
  71. ncbi Conserved receptor-binding domains of Lake Victoria marburgvirus and Zaire ebolavirus bind a common receptor
    Jens H Kuhn
    Department of Microbiology and Molecular Genetics, Harvard Medical School, New England Primate Research Center, Southborough, Massachusetts 01772, USA
    J Biol Chem 281:15951-8. 2006
    The GP(1,2) envelope glycoproteins (GP) of filoviruses (marburg- and ebolaviruses) mediate cell-surface attachment, membrane fusion, and entry into permissive cells...
  72. ncbi Filovirus budding
    Luke D Jasenosky
    Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin Madison, 2015 Linden Drive West, Madison, WI 53706, USA
    Virus Res 106:181-8. 2004
    ..Possible budding functions of the viral glycoprotein (GP), as well as the membrane-associated viral protein 24 (VP24), will also be described, and a model for filovirus ..
  73. pmc Reverse genetics demonstrates that proteolytic processing of the Ebola virus glycoprotein is not essential for replication in cell culture
    Gabriele Neumann
    Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin Madison, Madison, Wisconsin 53706, USA
    J Virol 76:406-10. 2002
    ..virus from cloned cDNA to engineer a mutant Ebola virus with an altered furin recognition motif in the glycoprotein (GP). When expressed in cells, the GP of the wild type, but not of the mutant, virus was cleaved into GP1 and GP2...
  74. ncbi Implication of a retrovirus-like glycoprotein peptide in the immunopathogenesis of Ebola and Marburg viruses
    Kavitha Yaddanapudi
    Jerome L and Dawn Greene Infectious Disease Laboratory, Mailman School of Public Health, Columbia University, 722 West 168th St, New York, NY 10032, USA
    FASEB J 20:2519-30. 2006
    ..In contrast, only rhesus T cells were sensitive to REBOV peptides. These findings are consistent with the observation that REBOV is not pathogenic in humans and have implications for understanding the pathogenesis of filoviral HF...