omp28

Summary

Gene Symbol: omp28
Description: immunoreactive 28 kDa outer membrane protein
Species: Brucella melitensis biovar Abortus 2308

Top Publications

  1. doi Optimization and efficient purification of recombinant Omp28 protein of Brucella melitensis using Triton X-100 and β-mercaptoethanol
    Ashu Kumar
    Microbiology Division, Defence Research and Development Establishment, Jhansi Road, Gwalior 474002, India
    Protein Expr Purif 83:226-32. 2012
  2. pmc Cloning of Brucella abortus gene and characterization of expressed 26-kilodalton periplasmic protein: potential use for diagnosis
    O L Rossetti
    Instituto de Biotecnologia, Centro de Investigacion en Ciencias Veterinarias, Buenos Aires, Argentina
    J Clin Microbiol 34:165-9. 1996
  3. ncbi Cloning, nucleotide sequence, and expression of the Brucella melitensis bp26 gene coding for a protein immunogenic in infected sheep
    A Cloeckaert
    Institut National de la Recherche Agronomique, Laboratoire de Pathologie Infectieuse et Immunologie, Nouzilly, France
    FEMS Microbiol Lett 140:139-44. 1996
  4. pmc An IS711 element downstream of the bp26 gene is a specific marker of Brucella spp. isolated from marine mammals
    A Cloeckaert
    Laboratoire de Pathologie Infectieuse et Immunologie, Institut National de la Recherche Agronomique, 37380 Nouzilly, France
    Clin Diagn Lab Immunol 7:835-9. 2000
  5. pmc Epitope mapping of the Brucella melitensis BP26 immunogenic protein: usefulness for diagnosis of sheep brucellosis
    Patricia Seco-Mediavilla
    Departamento de Microbiologia y Genetica, Universidad de Salamanca, 37007 Salamanca, Spain
    Clin Diagn Lab Immunol 10:647-51. 2003
  6. ncbi Molecular evidence of new variant Brucella in North Pacific common minke whales
    Kazue Ohishi
    Program for Marine Biology and Ecology, Japan Agency for Marine Earth Science and Technology, Natsushima 2 15, Yokosuka, Kanagawa 237 0061, Japan
    Microbes Infect 6:1199-204. 2004

Scientific Experts

  • A Cloeckaert
  • Kazue Ohishi
  • Ashu Kumar
  • Patricia Seco-Mediavilla
  • Archana Prakash
  • Sonia Arora
  • Sapana Tiwari
  • M Kameswara Rao
  • Anita Barua
  • Kannusamy Sathyaseelan
  • Duraipandian Thavaselvam
  • Maggy Grayon
  • Jean Michel Verger
  • Luis Fernández-Lago
  • Michel S Zygmunt
  • Nieves Vizcaino
  • Clara M Marin
  • O L Rossetti
  • S L Cravero
  • M L Boschiroli
  • A I Arese

Detail Information

Publications6

  1. doi Optimization and efficient purification of recombinant Omp28 protein of Brucella melitensis using Triton X-100 and β-mercaptoethanol
    Ashu Kumar
    Microbiology Division, Defence Research and Development Establishment, Jhansi Road, Gwalior 474002, India
    Protein Expr Purif 83:226-32. 2012
    ..In this study recombinant Omp28 (rOmp28) protein of Brucella melitensis was expressed in two different vector systems and further efficient ..
  2. pmc Cloning of Brucella abortus gene and characterization of expressed 26-kilodalton periplasmic protein: potential use for diagnosis
    O L Rossetti
    Instituto de Biotecnologia, Centro de Investigacion en Ciencias Veterinarias, Buenos Aires, Argentina
    J Clin Microbiol 34:165-9. 1996
    ..BP26 might be of value as an antigen for serological diagnosis of brucellosis in different mammals...
  3. ncbi Cloning, nucleotide sequence, and expression of the Brucella melitensis bp26 gene coding for a protein immunogenic in infected sheep
    A Cloeckaert
    Institut National de la Recherche Agronomique, Laboratoire de Pathologie Infectieuse et Immunologie, Nouzilly, France
    FEMS Microbiol Lett 140:139-44. 1996
    ..Immunoblotting performed with sera from either naturally infected or B. melitensis H38 experimentally infected sheep confirmed the importance of the B. melitensis CP28/BP26 protein as diagnostic antigen...
  4. pmc An IS711 element downstream of the bp26 gene is a specific marker of Brucella spp. isolated from marine mammals
    A Cloeckaert
    Laboratoire de Pathologie Infectieuse et Immunologie, Institut National de la Recherche Agronomique, 37380 Nouzilly, France
    Clin Diagn Lab Immunol 7:835-9. 2000
    ..Thus, this PCR assay is able to differentiate Brucella terrestrial isolates from marine mammal isolates and could be applied for diagnostic purposes...
  5. pmc Epitope mapping of the Brucella melitensis BP26 immunogenic protein: usefulness for diagnosis of sheep brucellosis
    Patricia Seco-Mediavilla
    Departamento de Microbiologia y Genetica, Universidad de Salamanca, 37007 Salamanca, Spain
    Clin Diagn Lab Immunol 10:647-51. 2003
    ..Thus, use of this recombinant fusion protein instead the entire recombinant BP26 could improve the specific serological diagnosis of B. melitensis or B. ovis infection in sheep...
  6. ncbi Molecular evidence of new variant Brucella in North Pacific common minke whales
    Kazue Ohishi
    Program for Marine Biology and Ecology, Japan Agency for Marine Earth Science and Technology, Natsushima 2 15, Yokosuka, Kanagawa 237 0061, Japan
    Microbes Infect 6:1199-204. 2004
    ..The North Pacific Brucella showed the highest similarity to North Atlantic seal strains among the known Brucella strains...