Michael A Whitt

Summary

Publications

  1. pmc Single injection recombinant vesicular stomatitis virus vaccines protect ferrets against lethal Nipah virus disease
    Chad E Mire
    Galveston National Laboratory, University of Texas Medical Branch, 301 University Blvd, Galveston, TX, USA
    Virol J 10:353. 2013
  2. ncbi request reprint Utilization of fluorescently-labeled tetracysteine-tagged proteins to study virus entry by live cell microscopy
    Michael A Whitt
    Department of Microbiology, Immunology and Biochemistry, University of Tennessee Health Science Center, Memphis, TN 38163, USA
    Methods 55:127-36. 2011
  3. pmc Generation of VSV pseudotypes using recombinant ΔG-VSV for studies on virus entry, identification of entry inhibitors, and immune responses to vaccines
    Michael A Whitt
    Department of Molecular Sciences, 858 Madison Ave, The University of Tennessee Health Science Center, Memphis, TN 38163, USA
    J Virol Methods 169:365-74. 2010
  4. ncbi request reprint IL-12-assisted immunization against Listeria monocytogenes using replication-restricted VSV-based vectors
    Sheri D Klas
    Department of Molecular Sciences, University of Tennessee Health Science Center, Memphis, TN 38163, USA
    Vaccine 24:1451-61. 2006
  5. ncbi request reprint Immunization with heat-killed Francisella tularensis LVS elicits protective antibody-mediated immunity
    Christy L Lavine
    University of Tennessee Health Science Center, Department of Molecular Sciences, Memphis, TN 38163, USA
    Eur J Immunol 37:3007-20. 2007
  6. pmc A spatio-temporal analysis of matrix protein and nucleocapsid trafficking during vesicular stomatitis virus uncoating
    Chad E Mire
    Department of Molecular Sciences, University of Tennessee Health Science Center, Memphis, Tennessee, United States of America
    PLoS Pathog 6:e1000994. 2010
  7. pmc Binding and activation of host plasminogen on the surface of Francisella tularensis
    Shawn R Clinton
    Department of Molecular Sciences, The University of Tennessee Health Science Center, 858 Madison Avenue, Memphis, Tennessee 38163, USA
    BMC Microbiol 10:76. 2010
  8. pmc Glycoprotein-dependent acidification of vesicular stomatitis virus enhances release of matrix protein
    Chad E Mire
    Department of Molecular Sciences, University of Tennessee Health Science Center, Memphis, Tennessee 38163, USA
    J Virol 83:12139-50. 2009
  9. ncbi request reprint Adjuvanticity of an IL-12 fusion protein expressed by recombinant deltaG-vesicular stomatitis virus
    Sheri D Klas
    Department of Molecular Sciences, University of Tennessee Health Science Center, 858 Madison Avenue, Memphis, TN 38163, USA
    Cell Immunol 218:59-73. 2002
  10. pmc The membrane-proximal region of vesicular stomatitis virus glycoprotein G ectodomain is critical for fusion and virus infectivity
    E Jeetendra
    Department of Molecular Sciences, University of Tennessee Health Sciences Center GTx, Inc, Memphis, Tennessee 38163, USA
    J Virol 77:12807-18. 2003

Collaborators

Detail Information

Publications19

  1. pmc Single injection recombinant vesicular stomatitis virus vaccines protect ferrets against lethal Nipah virus disease
    Chad E Mire
    Galveston National Laboratory, University of Texas Medical Branch, 301 University Blvd, Galveston, TX, USA
    Virol J 10:353. 2013
    ..Outbreaks have occurred in Malaysia, Singapore, India, and Bangladesh and have been associated with 40 to 75% case fatality rates. There are currently no vaccines or postexposure treatments licensed for combating human NiV infection...
  2. ncbi request reprint Utilization of fluorescently-labeled tetracysteine-tagged proteins to study virus entry by live cell microscopy
    Michael A Whitt
    Department of Microbiology, Immunology and Biochemistry, University of Tennessee Health Science Center, Memphis, TN 38163, USA
    Methods 55:127-36. 2011
    ....
  3. pmc Generation of VSV pseudotypes using recombinant ΔG-VSV for studies on virus entry, identification of entry inhibitors, and immune responses to vaccines
    Michael A Whitt
    Department of Molecular Sciences, 858 Madison Ave, The University of Tennessee Health Science Center, Memphis, TN 38163, USA
    J Virol Methods 169:365-74. 2010
    ..These protocols should allow any laboratory knowledgeable in general virological and cell culture techniques to produce successfully replication-restricted rVSV-ΔG pseudotypes for subsequent analysis...
  4. ncbi request reprint IL-12-assisted immunization against Listeria monocytogenes using replication-restricted VSV-based vectors
    Sheri D Klas
    Department of Molecular Sciences, University of Tennessee Health Science Center, Memphis, TN 38163, USA
    Vaccine 24:1451-61. 2006
    ..Here, we report that immunization of mice with a poorly immunogenic listerial antigen preparation (LMAg) in combination with VSVDeltaG-IL12F elicits potent T cell- and B cell-mediated responses that confer protective listerial immunity...
  5. ncbi request reprint Immunization with heat-killed Francisella tularensis LVS elicits protective antibody-mediated immunity
    Christy L Lavine
    University of Tennessee Health Science Center, Department of Molecular Sciences, Memphis, TN 38163, USA
    Eur J Immunol 37:3007-20. 2007
    ..These findings demonstrated that FT-specific antibodies alone can confer immunity against high-dose FTLVS challenge, and they reveal that antibody-mediated protection is not dependent upon production of LPS-specific antibodies...
  6. pmc A spatio-temporal analysis of matrix protein and nucleocapsid trafficking during vesicular stomatitis virus uncoating
    Chad E Mire
    Department of Molecular Sciences, University of Tennessee Health Science Center, Memphis, Tennessee, United States of America
    PLoS Pathog 6:e1000994. 2010
    ....
  7. pmc Binding and activation of host plasminogen on the surface of Francisella tularensis
    Shawn R Clinton
    Department of Molecular Sciences, The University of Tennessee Health Science Center, 858 Madison Avenue, Memphis, Tennessee 38163, USA
    BMC Microbiol 10:76. 2010
    ..A number of both gram-positive and gram-negative bacterial pathogens have the ability to bind to PLG, giving them a survival advantage by increasing their ability to penetrate extracellular matrices and cross tissue barriers...
  8. pmc Glycoprotein-dependent acidification of vesicular stomatitis virus enhances release of matrix protein
    Chad E Mire
    Department of Molecular Sciences, University of Tennessee Health Science Center, Memphis, Tennessee 38163, USA
    J Virol 83:12139-50. 2009
    ..From these data we propose that low-pH conformational changes in G protein promote acidification of the virus interior, which facilitates the release of M from ribonucleoprotein particles during uncoating...
  9. ncbi request reprint Adjuvanticity of an IL-12 fusion protein expressed by recombinant deltaG-vesicular stomatitis virus
    Sheri D Klas
    Department of Molecular Sciences, University of Tennessee Health Science Center, 858 Madison Avenue, Memphis, TN 38163, USA
    Cell Immunol 218:59-73. 2002
    ..These studies demonstrated that VSVdeltaG-IL12F-infected cells secrete bioactive single-chain IL-12, and laid the foundation for studies using VSVdeltaG-IL12F as a vector for delivery of IL-12F in vivo...
  10. pmc The membrane-proximal region of vesicular stomatitis virus glycoprotein G ectodomain is critical for fusion and virus infectivity
    E Jeetendra
    Department of Molecular Sciences, University of Tennessee Health Sciences Center GTx, Inc, Memphis, Tennessee 38163, USA
    J Virol 77:12807-18. 2003
    ..These data support the hypothesis that the membrane-proximal domain contributes to G-mediated membrane fusion activity, yet the conserved aromatic residues are not essential for membrane fusion or virus infectivity...
  11. ncbi request reprint Recombinant replication-restricted VSV as an expression vector for murine cytokines
    Mark A Miller
    Department of Molecular Sciences, University of Tennessee Health Science Center, 858 Madison Avenue, Memphis, TN 38163, USA
    Protein Expr Purif 33:92-103. 2004
    ..The results presented here reveal that large quantities of bioactive cytokines can be produced rapidly and inexpensively using deltaG-VSV as a protein expression system...
  12. pmc Inhibition of type I interferon-mediated antiviral action in human glioma cells by the IKK inhibitors BMS-345541 and TPCA-1
    Ziyun Du
    Department of Pathology and Laboratory Medicine, University of Tennessee Health Science Center, Memphis, Tennessee 38163, USA
    J Interferon Cytokine Res 32:368-77. 2012
    ..Since VSV and EMCV are oncolytic viruses used in cancer therapy, our results indicate the potential synergy in combining IKK inhibitors with oncolytic viruses...
  13. ncbi request reprint The protease-sensitive loop of the vesicular stomatitis virus matrix protein is involved in virus assembly and protein translation
    Chad E Mire
    Department of Microbiology, Immunology and Biochemistry, University of Tennessee Health Science Center, Memphis, TN 38163, USA
    Virology 416:16-25. 2011
    ..These results indicate that the LXD motif is involved in both virus assembly and VSV protein translation...
  14. ncbi request reprint A novel role for a YXXPhi motif in directing the caveolin-dependent sorting of membrane-spanning proteins
    Frank C Dorsey
    Department of Molecular Sciences, University of Tennessee Health Science Center, 858 Madison Avenue, Memphis, TN 38163, USA
    J Cell Sci 120:2544-54. 2007
    ..The Y(47)VEL tetrapeptide matches the sequence of a YXXPhi motif, and our results indicate a novel role for this motif in directing caveolin-dependent sorting...
  15. ncbi request reprint Rhabdovirus assembly and budding
    Himangi R Jayakar
    GTx Inc, 3 N Dunlap, Van Vleet Research Building, Memphis, TN 38163, USA
    Virus Res 106:117-32. 2004
    ....
  16. ncbi request reprint RNA interference is an antiviral defence mechanism in Caenorhabditis elegans
    Courtney Wilkins
    Department of Microbiology, University of Arkansas for Medical Sciences, Little Rock, Arkansas 72205, USA
    Nature 436:1044-7. 2005
    ..Because the RNAi response occurs in the absence of exogenously added VSV small interfering RNAs, these results show that RNAi is activated during VSV infection and that RNAi is a genuine antiviral immune defence mechanism in the worm...
  17. ncbi request reprint Recombinant vesicular stomatitis virus vectors as oncolytic agents in the treatment of high-grade gliomas in an organotypic brain tissue slice-glioma coculture model
    Christopher D Duntsch
    Department of Neurosurgery, The University of Tennessee Health Science Center, Memphis, Tennessee 38163, USA
    J Neurosurg 100:1049-59. 2004
    ....
  18. ncbi request reprint Role of the cyclic AMP-dependent protein kinase in homologous resensitization of the beta1-adrenergic receptor
    Lidia A Gardner
    Pharmacology and Molecular Sciences, University of Tennessee Health Sciences Center, 874 Union Avenue, Memphis, TN 38163, USA
    J Biol Chem 279:21135-43. 2004
    ..Therefore, PKA-mediated phosphorylation of G protein-coupled receptors might result in motif-dependent desensitization or resensitization...
  19. pmc The membrane-proximal domain of vesicular stomatitis virus G protein functions as a membrane fusion potentiator and can induce hemifusion
    E Jeetendra
    Department of Molecular Sciences, University of Tennessee Health Science Center, Memphis, Tennessee 38163, USA
    J Virol 76:12300-11. 2002
    ..To our knowledge, this is the first report which shows that a small domain of one viral glycoprotein can promote the fusion activity of other, unrelated viral glycoproteins...