Wei Hua Wang

Summary

Publications

  1. pmc Mosaic pregnancy after transfer of a "euploid" blastocyst screened by DNA microarray
    Ghassan Haddad
    Houston Fertility Institute, New Houston Health, 2500 Fondren Rd, Suite 350, Houston, TX 77063, USA
    J Ovarian Res 6:70. 2013
  2. pmc Arrested human embryos are more likely to have abnormal chromosomes than developing embryos from women of advanced maternal age
    Shu Tao Qi
    Key Laboratory of Major Obstetrics Diseases of Guangdong Province, The Third Hospital Affiliated to Guangzhou Medical University, Guangdong, China
    J Ovarian Res 7:65. 2014
  3. pmc The prevalence of chromosomal deletions relating to developmental delay and/or intellectual disability in human euploid blastocysts
    Wenyin He
    Key Laboratory of Major Obstetrics Diseases of Guangdong Province, the Third Affiliated Hospital of Guangzhou Medical University, Guangdong, China
    PLoS ONE 9:e85207. 2014
  4. pmc Optimized protocol for cryopreservation of human eggs improves developmental competence and implantation of resulting embryos
    Cassie T Wang
    Houston Fertility Institute New Houston Health, Houston, TX, USA
    J Ovarian Res 6:15. 2013
  5. ncbi request reprint Development of porcine embryos produced by IVM/IVF in a medium with or without protein supplementation: effects of extracellular glutathione
    Wei Hua Wang
    Institute of Zoology, Chinese Academy of Science, Beijing
    Zygote 10:109-15. 2002
  6. ncbi request reprint Human embryonic stem cell lines are contaminated: what should we do?
    Wei Hua Wang
    In Vitro Fertilization Laboratory, Tomball Regional Hospital, TX 77375, USA
    Hum Reprod 20:2987-9. 2005
  7. ncbi request reprint A simplified technique for embryo biopsy for preimplantation genetic diagnosis
    Wei Hua Wang
    Houston Fertility Institute, Tomball Regional Hospital, Tomball, Texas 77375, USA
    Fertil Steril 90:438-42. 2008
  8. ncbi request reprint How does polyspermy happen in mammalian oocytes?
    Wei Hua Wang
    State Key Laboratory of Reproductive Biology, Institute of Zoology, Beijing 100080, China
    Microsc Res Tech 61:335-41. 2003
  9. doi request reprint Comparison of development and implantation of human embryos biopsied with two different methods: aspiration and displacement
    Wei Hua Wang
    Houston Fertility Institute Tomball Regional Hospital, Tomball, Texas 77375, USA
    Fertil Steril 92:536-40. 2009
  10. ncbi request reprint Prediction of chromosome misalignment among in vitro matured human oocytes by spindle imaging with the PolScope
    Wei Hua Wang
    Houston Fertility Institute, Tomball Regional Hospital, Tomball, Texas 77375, USA
    Fertil Steril 78:1077-81. 2002

Collaborators

Detail Information

Publications36

  1. pmc Mosaic pregnancy after transfer of a "euploid" blastocyst screened by DNA microarray
    Ghassan Haddad
    Houston Fertility Institute, New Houston Health, 2500 Fondren Rd, Suite 350, Houston, TX 77063, USA
    J Ovarian Res 6:70. 2013
    ..However, mosaic pregnancy is still possible due to embryonic mosacism. Here we report a successful pregnancy after transfer of a mosaic blastocyst with euploid inner cell mass...
  2. pmc Arrested human embryos are more likely to have abnormal chromosomes than developing embryos from women of advanced maternal age
    Shu Tao Qi
    Key Laboratory of Major Obstetrics Diseases of Guangdong Province, The Third Hospital Affiliated to Guangzhou Medical University, Guangdong, China
    J Ovarian Res 7:65. 2014
    ..Currently, chromosome screening for aneuploidy is performed in developing embryos, mainly blastocysts. It has not been performed in arrested embryos and/or compared between developing embryos and arrested embryos from the same IVF cycle...
  3. pmc The prevalence of chromosomal deletions relating to developmental delay and/or intellectual disability in human euploid blastocysts
    Wenyin He
    Key Laboratory of Major Obstetrics Diseases of Guangdong Province, the Third Affiliated Hospital of Guangzhou Medical University, Guangdong, China
    PLoS ONE 9:e85207. 2014
    ..Therefore, the embryos with chromosomal deletion revealed by DNA microarray should not be transferred to the patients, or further gene map and/or phenotype seeking is necessary before making a final decision. ..
  4. pmc Optimized protocol for cryopreservation of human eggs improves developmental competence and implantation of resulting embryos
    Cassie T Wang
    Houston Fertility Institute New Houston Health, Houston, TX, USA
    J Ovarian Res 6:15. 2013
    ..The present study was designed to examine if human embryos resulting from eggs frozen with an optimized vitrification protocol have similar developmental competence as those from fresh eggs...
  5. ncbi request reprint Development of porcine embryos produced by IVM/IVF in a medium with or without protein supplementation: effects of extracellular glutathione
    Wei Hua Wang
    Institute of Zoology, Chinese Academy of Science, Beijing
    Zygote 10:109-15. 2002
    ..These results indicate that pig embryos produced by IVM/IVF can develop to the blastocyst stage in a defined medium. BSA and glutathione have a synergistic effect on pig embryo development...
  6. ncbi request reprint Human embryonic stem cell lines are contaminated: what should we do?
    Wei Hua Wang
    In Vitro Fertilization Laboratory, Tomball Regional Hospital, TX 77375, USA
    Hum Reprod 20:2987-9. 2005
    ..Safe and fast approaches to producing hES cell lines based on recent research advances in both humans and animals have been suggested...
  7. ncbi request reprint A simplified technique for embryo biopsy for preimplantation genetic diagnosis
    Wei Hua Wang
    Houston Fertility Institute, Tomball Regional Hospital, Tomball, Texas 77375, USA
    Fertil Steril 90:438-42. 2008
    ..To report a simplified embryo biopsy method for preimplantation genetic diagnosis (PGD)...
  8. ncbi request reprint How does polyspermy happen in mammalian oocytes?
    Wei Hua Wang
    State Key Laboratory of Reproductive Biology, Institute of Zoology, Beijing 100080, China
    Microsc Res Tech 61:335-41. 2003
    ..Species-specific differences are present regarding the relationship between insemination conditions and polyspermy...
  9. doi request reprint Comparison of development and implantation of human embryos biopsied with two different methods: aspiration and displacement
    Wei Hua Wang
    Houston Fertility Institute Tomball Regional Hospital, Tomball, Texas 77375, USA
    Fertil Steril 92:536-40. 2009
    ..To compare the development and implantation of human embryos biopsied with two different methods for preimplantation genetic diagnosis (PGD)...
  10. ncbi request reprint Prediction of chromosome misalignment among in vitro matured human oocytes by spindle imaging with the PolScope
    Wei Hua Wang
    Houston Fertility Institute, Tomball Regional Hospital, Tomball, Texas 77375, USA
    Fertil Steril 78:1077-81. 2002
    ....
  11. ncbi request reprint Intra-oocyte localization of MAD2 and its relationship with kinetochores, microtubules, and chromosomes in rat oocytes during meiosis
    Dong Zhang
    State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, China
    Biol Reprod 71:740-8. 2004
    ..However, in this case, MAD2 could not check unaligned chromosomes in the recovered spindles, suggesting that a normal chromosome alignment is maintained only in the oocytes without any microtubule damages during maturation...
  12. ncbi request reprint Reduced expression of MAD2, BCL2, and MAP kinase activity in pig oocytes after in vitro aging are associated with defects in sister chromatid segregation during meiosis II and embryo fragmentation after activation
    Wei Ma
    State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, China
    Biol Reprod 72:373-83. 2005
    ..These events in turn cause abnormal sister chromatid segregation during meiosis II, embryo fragmentation, and apoptosis...
  13. ncbi request reprint Localization of mitotic arrest deficient 1 (MAD1) in mouse oocytes during the first meiosis and its functions as a spindle checkpoint protein
    Dong Zhang
    State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, China
    Biol Reprod 72:58-68. 2005
    ..However, MAD1 could not check misaligned chromosomes during spindle recovery after the spindles were destroyed by drug or cooling, which caused some chromosomes to scatter in the oocytes...
  14. ncbi request reprint Improved isolation and culture of embryonic stem cells from Chinese miniature pig
    Ming Li
    State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China
    J Reprod Dev 50:237-44. 2004
    ..Successful culture of ICM to ES-like cells has been achieved not only in MEF, but also in homologous (pig) feeder layer. The ES cells obtained in the present study were pluripotent...
  15. ncbi request reprint Maturation of porcine oocytes after cooling at the germinal vesicle stage
    Rui hua Liu
    State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Science, Beijing 100080, China
    Zygote 11:299-305. 2003
    ..Cooling oocytes before maturation inhibits their subsequent spindle organisation, nuclear and cytoplasmic maturation. Addition of DMSO to the cooling solution did not protect porcine oocytes from cooling-induced damage...
  16. ncbi request reprint Isolation and culture of pluripotent cells from in vitro produced porcine embryos
    Ming Li
    State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China
    Zygote 12:43-8. 2004
    ..The culture system used in the present study is useful to isolate and culture ES cells from in vitro produced porcine embryos...
  17. ncbi request reprint Isolation and culture of embryonic stem cells from porcine blastocysts
    Ming Li
    State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China
    Mol Reprod Dev 65:429-34. 2003
    ..Some cells formed embryoid bodies in a suspension culture. These results indicate that porcine ES cell line can be established under the present experimental conditions and these ES cells are pluripotent...
  18. ncbi request reprint Localization of CD9 in pig oocytes and its effects on sperm-egg interaction
    Yong Hai Li
    State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, China
    Reproduction 127:151-7. 2004
    ..5 +/- 0.4 per oocyte and 70.3% respectively). These results indicated that CD9 is expressed in pig oocytes during early growth and meiotic maturation and that it participates in sperm-oocyte interactions during fertilization...
  19. ncbi request reprint Reduced polyspermic penetration in porcine oocytes inseminated in a new in vitro fertilization (IVF) system: straw IVF
    Yong Hai Li
    State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, People s Republic of China
    Biol Reprod 69:1580-5. 2003
    ..These findings indicate that IVF of porcine oocytes in the straws provides a better condition in which more oocytes are fertilized normally than that in the microdrop IVF...
  20. pmc Assessment of aneuploidy formation in human blastocysts resulting from donated eggs and the necessity of the embryos for aneuploidy screening
    Ghassan Haddad
    Houston Fertility Institute, Houston, TX, USA
    J Assist Reprod Genet 32:999-1006. 2015
    ..Also, to assess the necessity of preimplantation genetic screening (PGS) for embryos produced with donor eggs...
  21. pmc Identification of chromosomal errors in human preimplantation embryos with oligonucleotide DNA microarray
    Lifeng Liang
    Houston Fertility Institute, Houston, Texas, United States of America
    PLoS ONE 8:e61838. 2013
    ..Preimplantation genetic screening of the aneuploidy by DNA microarray is an advanced technology used to select embryos for transfer and improved embryo implantation can be obtained after transfer of the screened normal embryos...
  22. ncbi request reprint Meiotic spindle, spindle checkpoint and embryonic aneuploidy
    Wei Hua Wang
    IVF Laboratory, Tomball Regional Hospital, Tomball, TX 77375, USA
    Front Biosci 11:620-36. 2006
    ..Furthermore, the causes of embryonic aneuploidies and diagnosis are discussed...
  23. ncbi request reprint Effects of cooling on meiotic spindle structure and chromosome alignment within in vitro matured porcine oocytes
    Rui hua Liu
    State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Science, Beijing 100080, China
    Mol Reprod Dev 65:212-8. 2003
    ..Both spindle and chromosomes were damaged during cooling, and such damage was not reversible by incubating the oocytes after they had been cooled...
  24. ncbi request reprint Rigorous thermal control during intracytoplasmic sperm injection stabilizes the meiotic spindle and improves fertilization and pregnancy rates
    Wei Hua Wang
    Houston Fertility Institute, Tomball Regional Hospital, Tomball, Texas, USA
    Fertil Steril 77:1274-7. 2002
    ..Woburn, MA)...
  25. ncbi request reprint Extracellular and intracellular factors affecting nuclear and cytoplasmic maturation of porcine oocytes collected from different sizes of follicles
    Rui hua Liu
    Institute of Zoology, Chinese Academy of Science, Beijing
    Zygote 10:253-60. 2002
    ....
  26. ncbi request reprint Spindle dynamics in living mouse oocytes during meiotic maturation, ageing, cooling and overheating: a study by polarized light microscopy
    Xiao Fang Sun
    Guangzhou Medical College, Guangzhou, China and Houston Fertility Institute, Tomball, Texas, USA
    Zygote 12:241-9. 2004
    ..These results also indicate that the M-II spindle in mouse oocytes is sensitive to oocyte ageing and cooling, but not heating, and M-I spindle is more sensitive to temperature decline than M-II spindle...
  27. ncbi request reprint Spindle imaging in living mammalian oocytes using the liquid-crystal polarized light microscope and its practical use
    Wei Hua Wang
    Houston Fertility Institute, Tomball Regional Hospital, TX, USA
    Methods Mol Biol 253:215-25. 2004
  28. ncbi request reprint Spindle observation in living mammalian oocytes with the polarization microscope and its practical use
    Wei Hua Wang
    State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China
    Cloning Stem Cells 4:269-76. 2002
    ..The Polscope can also be used to examine the spindle dynamics, detect spindle morphology, predict chromosome misalignment, and perform spindle transfer...
  29. ncbi request reprint In vitro maturation and in vitro fertilization of human oocytes
    Wei Hua Wang
    Houston Fertility Institute, Tomball Regional Hospital, TX, USA
    Methods Mol Biol 253:235-53. 2004
  30. ncbi request reprint Clear cell hidradenoma: a mimic of metastatic clear cell tumors
    Keith E Volmar
    Department of Pathology, Duke University Medical Center, Durham, NC 27599, USA
    Arch Pathol Lab Med 129:e113-6. 2005
    ..We report the rare case of a patient with renal cell carcinoma who underwent excision of an axillary clear cell hidradenoma, which was clinically suggestive of cutaneous metastatic disease...
  31. ncbi request reprint Cytoplasmic dynein participates in meiotic checkpoint inactivation in mouse oocytes by transporting cytoplasmic mitotic arrest-deficient (Mad) proteins from kinetochores to spindle poles
    Dong Zhang
    State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, China
    Reproduction 133:685-95. 2007
    ..These results indicate that dynein may play a role in spindle checkpoint inactivation...
  32. ncbi request reprint Overheating is detrimental to meiotic spindles within in vitro matured human oocytes
    Xiao Fang Sun
    Guang Zhou Second Hospital, Gung Dong, China
    Zygote 12:65-70. 2004
    ..These results indicate that spindles in human eggs are sensitive to high temperature. Moreover, maintenance of an in vitro manipulation temperature of 37 degrees C is crucial for normal spindle morphology...
  33. ncbi request reprint Improved development by Taxol pretreatment after vitrification of in vitro matured porcine oocytes
    Wen Qing Shi
    Laboratory of Animal Embryonic Biotechnology, College of Animal Science, China Agricultural University No 2 Yuanmingyuan West Road, Haidian District, Beijing 100094, PR China
    Reproduction 131:795-804. 2006
    ..6%) Taxol. These results indicate that pretreatment of oocytes with Taxol before vitrification helps to reduce the damage induced by vitrification and is a potential way to improve the development of vitrified porcine oocytes...
  34. ncbi request reprint A review of the cost-effectiveness of Tc-99m sestamibi scintimammography in diagnosis of breast cancer in Taiwanese women with indeterminate mammographically dense breast
    Yi Shan Chen
    Institute of Industrial Engineering and Enterprise Information of Tunghai University, Taichung, Taiwan
    Surg Oncol 11:151-5. 2002
    ..The analysis data indicate that Tc-99m sestamibi scintimammography can save the cost of unnecessary biopsies in Taiwanese women with non-diagnostic mammogram because of mammographically dense breasts...
  35. ncbi request reprint [Construction of a gastric cancer related gene GCRG213-specific siRNA expression vector and its effect on gastric cancer cells in vitro]
    Li li Gao
    Department of Gastroenterology, General Hospital of PLA, Beijing 100853, China
    Zhonghua Zhong Liu Za Zhi 29:84-8. 2007
    ..To investigate the effect of gene GCRG213 siRNA transfection into gastric cancer cell line MKN45 cells...
  36. ncbi request reprint [The clinical and pathological features of rhinocerebral mucormycosis]
    Hong Guo
    Department of Neurology, Beijing Hospital, Beijing 100730, China
    Zhonghua Nei Ke Za Zhi 43:686-9. 2004
    ..To investigate the clinical and pathological features of rhinocerebral mucormycosis...