Xiaotian Zhong

Summary

Affiliation: Wyeth Research
Country: USA

Publications

  1. ncbi request reprint Distinctive roles of endoplasmic reticulum and golgi glycosylation in functional surface expression of mammalian E-NTPDase1, CD39
    Xiaotian Zhong
    Department of Chemical and Screening Sciences, Wyeth Research, 85 Bolton Street, Cambridge, MA 02140, United States
    Biochim Biophys Acta 1723:143-50. 2005
  2. ncbi request reprint A eukaryotic carboxyl-terminal signal sequence translocating large hydrophilic domains across membranes
    Xiaotian Zhong
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA
    FEBS Lett 579:5643-50. 2005
  3. ncbi request reprint Regulation of secreted Frizzled-related protein-1 by heparin
    Xiaotian Zhong
    Department of Chemical and Screening Sciences, Wyeth Research, 200 Cambridge Park Drive, Cambridge, MA 02140, USA
    J Biol Chem 282:20523-33. 2007
  4. pmc Expression, purification and crystallization of the ecto-enzymatic domain of rat E-NTPDase1 CD39
    Xiaotian Zhong
    Department of Chemical and Screening Sciences, Wyeth Research, 200 Cambridge Park Drive, Cambridge, MA 02140, USA
    Acta Crystallogr Sect F Struct Biol Cryst Commun 64:1063-5. 2008
  5. doi request reprint Swift residue-screening identifies key N-glycosylated asparagines sufficient for surface expression of neuroglycoprotein Lingo-1
    Xiaotian Zhong
    Department of Chemical and Screening Sciences, Wyeth Research, 200 Cambridge Park Drive, Cambridge, MA 02140, USA
    FEBS Lett 583:1034-8. 2009
  6. ncbi request reprint ATP uptake in the Golgi and extracellular release require Mcd4 protein and the vacuolar H+-ATPase
    Xiaotian Zhong
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138, USA
    J Biol Chem 278:33436-44. 2003
  7. ncbi request reprint The structure of the Lingo-1 ectodomain, a module implicated in central nervous system repair inhibition
    Lidia Mosyak
    Department of Chemical and Screening Sciences, Wyeth Research, Cambridge, Massachusetts 02140, USA
    J Biol Chem 281:36378-90. 2006
  8. ncbi request reprint N-linked glycosylation of platelet P2Y12 ADP receptor is essential for signal transduction but not for ligand binding or cell surface expression
    Xiaotian Zhong
    Department of Chemical and Screening Sciences, Wyeth Research, 85 Bolton Street, Cambridge, MA 02140, USA
    FEBS Lett 562:111-7. 2004
  9. doi request reprint Engineering novel Lec1 glycosylation mutants in CHO-DUKX cells: molecular insights and effector modulation of N-acetylglucosaminyltransferase I
    Xiaotian Zhong
    Global Biotherapeutics Technologies, Pfizer Biotherapeutic Research and Development, 87 Cambridge Park Drive, Cambridge, Massachusetts 02140, USA
    Biotechnol Bioeng 109:1723-34. 2012
  10. pmc Crystal structures of the two major aggrecan degrading enzymes, ADAMTS4 and ADAMTS5
    Lidia Mosyak
    Department of Chemical and Screening Sciences, Wyeth Research, Cambridge, MA 02140, USA
    Protein Sci 17:16-21. 2008

Collaborators

Detail Information

Publications12

  1. ncbi request reprint Distinctive roles of endoplasmic reticulum and golgi glycosylation in functional surface expression of mammalian E-NTPDase1, CD39
    Xiaotian Zhong
    Department of Chemical and Screening Sciences, Wyeth Research, 85 Bolton Street, Cambridge, MA 02140, United States
    Biochim Biophys Acta 1723:143-50. 2005
    ..The study has also shown that N-linked glycosylation of CD39 is dispensable for the activity after the protein is properly folded and targeted...
  2. ncbi request reprint A eukaryotic carboxyl-terminal signal sequence translocating large hydrophilic domains across membranes
    Xiaotian Zhong
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA
    FEBS Lett 579:5643-50. 2005
    ..When placed at the C-terminal end, the sequence can translocate large extracellular domains of two membrane proteins across the membranes. The data demonstrate the existence of a true eukaryotic C-terminal signal sequence...
  3. ncbi request reprint Regulation of secreted Frizzled-related protein-1 by heparin
    Xiaotian Zhong
    Department of Chemical and Screening Sciences, Wyeth Research, 200 Cambridge Park Drive, Cambridge, MA 02140, USA
    J Biol Chem 282:20523-33. 2007
    ..The study has revealed a multifaceted regulation on sFRP-1 protein by heparin...
  4. pmc Expression, purification and crystallization of the ecto-enzymatic domain of rat E-NTPDase1 CD39
    Xiaotian Zhong
    Department of Chemical and Screening Sciences, Wyeth Research, 200 Cambridge Park Drive, Cambridge, MA 02140, USA
    Acta Crystallogr Sect F Struct Biol Cryst Commun 64:1063-5. 2008
    ..2 A data set using a maximum-likelihood molecular-replacement method as implemented in Phaser. The initial model of the two sCD39 monomers was placed into the P3(2) lattice and rigid-body refined and position-minimized with PHENIX...
  5. doi request reprint Swift residue-screening identifies key N-glycosylated asparagines sufficient for surface expression of neuroglycoprotein Lingo-1
    Xiaotian Zhong
    Department of Chemical and Screening Sciences, Wyeth Research, 200 Cambridge Park Drive, Cambridge, MA 02140, USA
    FEBS Lett 583:1034-8. 2009
    ..The swift residue-screening approach may provide a new strategy for structural and functional analysis...
  6. ncbi request reprint ATP uptake in the Golgi and extracellular release require Mcd4 protein and the vacuolar H+-ATPase
    Xiaotian Zhong
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138, USA
    J Biol Chem 278:33436-44. 2003
    ....
  7. ncbi request reprint The structure of the Lingo-1 ectodomain, a module implicated in central nervous system repair inhibition
    Lidia Mosyak
    Department of Chemical and Screening Sciences, Wyeth Research, Cambridge, Massachusetts 02140, USA
    J Biol Chem 281:36378-90. 2006
    ..Potential functional binding sites that can be identified on the ectodomain surface, including the site of self-recognition, suggest a model for protein assembly on the membrane...
  8. ncbi request reprint N-linked glycosylation of platelet P2Y12 ADP receptor is essential for signal transduction but not for ligand binding or cell surface expression
    Xiaotian Zhong
    Department of Chemical and Screening Sciences, Wyeth Research, 85 Bolton Street, Cambridge, MA 02140, USA
    FEBS Lett 562:111-7. 2004
    ..Thus the study uncovers an unexpected vital role of N-linked glycans in receptor's signal transducing step but not in surface expression or ligand binding...
  9. doi request reprint Engineering novel Lec1 glycosylation mutants in CHO-DUKX cells: molecular insights and effector modulation of N-acetylglucosaminyltransferase I
    Xiaotian Zhong
    Global Biotherapeutics Technologies, Pfizer Biotherapeutic Research and Development, 87 Cambridge Park Drive, Cambridge, Massachusetts 02140, USA
    Biotechnol Bioeng 109:1723-34. 2012
    ..The data support the hypothesis that modulating GnTI activity can influence antibody effector functions for proteins with an IgG1 immunoglobulin Fc domain...
  10. pmc Crystal structures of the two major aggrecan degrading enzymes, ADAMTS4 and ADAMTS5
    Lidia Mosyak
    Department of Chemical and Screening Sciences, Wyeth Research, Cambridge, MA 02140, USA
    Protein Sci 17:16-21. 2008
    ..On this basis, we propose that mature aggrecanases exist as an ensemble of at least two isomers, only one of which is proteolytically active...
  11. doi request reprint H6PDH interacts directly with 11beta-HSD1: implications for determining the directionality of glucocorticoid catalysis
    Yan Ling Zhang
    Cardiovascular and Metabolic Diseases, Wyeth Research, Cambridge, MA 02140, USA
    Arch Biochem Biophys 483:45-54. 2009
    ..The NTD retains the ability to interact with 11beta-HSD1 physically as well as modulate 11beta-HSD1 directionality indicating that the NTD of H6PDH is sufficient for the regulation of the 11beta-HSD1 activity...
  12. pmc Pyroglutamate and O-linked glycan determine functional production of anti-IL17A and anti-IL22 peptide-antibody bispecific genetic fusions
    Xiaotian Zhong
    Pfizer BioTherapeutics Research and Development, Cambridge, Massachusetts 02140, USA
    J Biol Chem 288:1409-19. 2013
    ..The newly engineered peptide-antibody genetic fusion is promising for therapeutically targeting multiple antigens in a single antibody-like molecule...