Genomes and Genes
Affiliation: Washington University School of Medicine
- Translocation of Saccharomyces cerevisiae Pif1 helicase monomers on single-stranded DNARoberto Galletto
252 McDonnell Science Building, Department of Biochemistry and Molecular Biophysics, Washington University, School of Medicine, 660 South Euclid Avenue, MS8231, Saint Louis, MO 63110
Nucleic Acids Res 41:4613-27. 2013..Finally, although we show that Pif1 can translocate on ssDNA, the translocation profiles suggest the presence on ssDNA of two populations of Pif1, both able to translocate with 5' to 3' directionality...
- Chemo-mechanical pushing of proteins along single-stranded DNAJOSHUA E SOKOLOSKI
Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St Louis, MO 63110
Proc Natl Acad Sci U S A 113:6194-9. 2016..The ability of translocases to chemo-mechanically push heterologous SSB proteins along ssDNA provides a potential mechanism for reorganization and clearance of tightly bound SSBs from ssDNA...
- DNA binding induces dimerization of Saccharomyces cerevisiae Pif1Sergio Barranco-Medina
Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, Saint Louis, Missouri 63110, USA
Biochemistry 49:8445-54. 2010..The observation that a Pif1 dimer is formed on unwinding substrates in the presence of ATP analogues suggests that a dimeric form of the enzyme might constitute the pre-initiation complex leading to its unwinding activity...
- A Monomer of Pif1 Unwinds Double-Stranded DNA and It Is Regulated by the Nature of the Non-Translocating Strand at the 3'-EndSaurabh P Singh
Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, Saint Louis, MO 63110, USA
J Mol Biol 428:1053-67. 2016..We propose that, in this case, binding of DNA to the second site is precluded and modulation of the Pif1-dependent re-winding activity is largely lost...