Carol A Rouzer

Summary

Affiliation: Vanderbilt University
Country: USA

Publications

  1. ncbi request reprint Chemical stability of 2-arachidonylglycerol under biological conditions
    Carol A Rouzer
    Department of Biochemistry, Vanderbilt Ingram Cancer Center, Vanderbilt University School of Medicine, Nashville, TN 37232 0146, USA
    Chem Phys Lipids 119:69-82. 2002
  2. ncbi request reprint Lipid profiling reveals glycerophospholipid remodeling in zymosan-stimulated macrophages
    Carol A Rouzer
    Department of Biochemistry, Center in Molecular Toxicology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 0146, USA
    Biochemistry 46:6026-42. 2007
  3. pmc Zymosan-induced glycerylprostaglandin and prostaglandin synthesis in resident peritoneal macrophages: roles of cyclo-oxygenase-1 and -2
    Carol A Rouzer
    Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, TN 37232 0146, USA
    Biochem J 399:91-9. 2006
  4. ncbi request reprint Glycerylprostaglandin synthesis by resident peritoneal macrophages in response to a zymosan stimulus
    Carol A Rouzer
    Department of Biochemistry, the Vanderbilt Institute of Chemical Biology, the Center in Molecular Toxicology, Nashville, TN 37232 0146, USA
    J Biol Chem 280:26690-700. 2005
  5. pmc Differential sensitivity and mechanism of inhibition of COX-2 oxygenation of arachidonic acid and 2-arachidonoylglycerol by ibuprofen and mefenamic acid
    Jeffery J Prusakiewicz
    Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 0146, USA
    Biochemistry 48:7353-5. 2009
  6. pmc Oxidative metabolism of a fatty acid amide hydrolase-regulated lipid, arachidonoyltaurine
    MELISSA V TURMAN
    A B Hancock, Jr Memorial Laboratory for Cancer Research, Department of Biochemistry, Vanderbilt Institute of Chemical Biology, and Center in Molecular Toxicology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 0146, USA
    Biochemistry 47:3917-25. 2008
  7. ncbi request reprint RAW264.7 cells lack prostaglandin-dependent autoregulation of tumor necrosis factor-alpha secretion
    Carol A Rouzer
    Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, TN 37232 0146, USA
    J Lipid Res 46:1027-37. 2005
  8. pmc Non-redundant functions of cyclooxygenases: oxygenation of endocannabinoids
    Carol A Rouzer
    A B Hancock Jr Memorial Laboratory for Cancer Research, The Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, TN 37232 0146, USA
    J Biol Chem 283:8065-9. 2008
  9. pmc Lipid profiling reveals arachidonate deficiency in RAW264.7 cells: Structural and functional implications
    Carol A Rouzer
    Department of Biochemistry, Center in Molecular Toxicology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 0146, USA
    Biochemistry 45:14795-808. 2006
  10. ncbi request reprint Simultaneous analysis of prostaglandin glyceryl esters and prostaglandins by electrospray tandem mass spectrometry
    Philip J Kingsley
    Department of Biochemistry and Department of Chemistry, Vanderbilt Institute of Chemical Biology, Vanderbilt University School of Medicine, Nashville, TN 37232, USA
    Anal Biochem 343:203-11. 2005

Collaborators

Detail Information

Publications22

  1. ncbi request reprint Chemical stability of 2-arachidonylglycerol under biological conditions
    Carol A Rouzer
    Department of Biochemistry, Vanderbilt Ingram Cancer Center, Vanderbilt University School of Medicine, Nashville, TN 37232 0146, USA
    Chem Phys Lipids 119:69-82. 2002
    ....
  2. ncbi request reprint Lipid profiling reveals glycerophospholipid remodeling in zymosan-stimulated macrophages
    Carol A Rouzer
    Department of Biochemistry, Center in Molecular Toxicology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 0146, USA
    Biochemistry 46:6026-42. 2007
    ..These results suggest that GPCho is the major ultimate source of 20:4 that is mobilized in zymosan-stimulated RPMs but that 20:4 mobilization may involve the intermediate turnover of alkyl acyl GPEtn species...
  3. pmc Zymosan-induced glycerylprostaglandin and prostaglandin synthesis in resident peritoneal macrophages: roles of cyclo-oxygenase-1 and -2
    Carol A Rouzer
    Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, TN 37232 0146, USA
    Biochem J 399:91-9. 2006
    ..They also indicate that the 2-AG and AA used for PG-G and PG synthesis respectively are derived from independent pathways...
  4. ncbi request reprint Glycerylprostaglandin synthesis by resident peritoneal macrophages in response to a zymosan stimulus
    Carol A Rouzer
    Department of Biochemistry, the Vanderbilt Institute of Chemical Biology, the Center in Molecular Toxicology, Nashville, TN 37232 0146, USA
    J Biol Chem 280:26690-700. 2005
    ..In conclusion, lipopolysaccharide-pretreated macrophages produce PG-Gs from endogenous 2-AG during zymosan phagocytosis, but PG-G formation is limited by substrate hydrolysis and inactivation of COX-2...
  5. pmc Differential sensitivity and mechanism of inhibition of COX-2 oxygenation of arachidonic acid and 2-arachidonoylglycerol by ibuprofen and mefenamic acid
    Jeffery J Prusakiewicz
    Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 0146, USA
    Biochemistry 48:7353-5. 2009
    ..In contrast, ibuprofen and mefenamate must bind in both subunits to inhibit AA binding...
  6. pmc Oxidative metabolism of a fatty acid amide hydrolase-regulated lipid, arachidonoyltaurine
    MELISSA V TURMAN
    A B Hancock, Jr Memorial Laboratory for Cancer Research, Department of Biochemistry, Vanderbilt Institute of Chemical Biology, and Center in Molecular Toxicology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 0146, USA
    Biochemistry 47:3917-25. 2008
    ..Over prolonged incubations, RPMs also generated small amounts of diHETE-T. Oxidative metabolism of polyunsaturated N-acyltaurines may represent a pathway for the generation or termination of novel signaling molecules...
  7. ncbi request reprint RAW264.7 cells lack prostaglandin-dependent autoregulation of tumor necrosis factor-alpha secretion
    Carol A Rouzer
    Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, TN 37232 0146, USA
    J Lipid Res 46:1027-37. 2005
    ....
  8. pmc Non-redundant functions of cyclooxygenases: oxygenation of endocannabinoids
    Carol A Rouzer
    A B Hancock Jr Memorial Laboratory for Cancer Research, The Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, TN 37232 0146, USA
    J Biol Chem 283:8065-9. 2008
    ..These compounds are produced in intact cells stimulated with physiological agonists and have been isolated from in vivo sources. Important concepts relevant to the hypothesis of a COX-2-selective signaling pathway are presented...
  9. pmc Lipid profiling reveals arachidonate deficiency in RAW264.7 cells: Structural and functional implications
    Carol A Rouzer
    Department of Biochemistry, Center in Molecular Toxicology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 0146, USA
    Biochemistry 45:14795-808. 2006
    ....
  10. ncbi request reprint Simultaneous analysis of prostaglandin glyceryl esters and prostaglandins by electrospray tandem mass spectrometry
    Philip J Kingsley
    Department of Biochemistry and Department of Chemistry, Vanderbilt Institute of Chemical Biology, Vanderbilt University School of Medicine, Nashville, TN 37232, USA
    Anal Biochem 343:203-11. 2005
    ..This method allows simultaneous profiling of several PG-Gs and PGs without multistep sample purification or derivatization...
  11. ncbi request reprint Structural and functional differences between cyclooxygenases: fatty acid oxygenases with a critical role in cell signaling
    Carol A Rouzer
    Department of Biochemistry, Vanderbilt Institute of Chemical Biology, Vanderbilt Ingram Cancer Center, Vanderbilt University School of Medicine, Nashville, TN 37232 0146, USA
    Biochem Biophys Res Commun 338:34-44. 2005
    ..These findings suggest that PG-Gs comprise a new class of lipid mediators, and that oxygenation of neutral derivatives of AA is a distinct function for the COX-2 isoform...
  12. pmc Competition and allostery govern substrate selectivity of cyclooxygenase-2
    Michelle M Mitchener
    A B Hancock Memorial Laboratory for Cancer Research, Vanderbilt University School of Medicine, Nashville, TN 37232 Department of Chemistry, Vanderbilt University School of Medicine, Nashville, TN 37232 The Vanderbilt Institute of Chemical Biology, Vanderbilt University School of Medicine, Nashville, TN 37232 Vanderbilt Ingram Cancer Center, Vanderbilt University School of Medicine, Nashville, TN 37232
    Proc Natl Acad Sci U S A 112:12366-71. 2015
    ..Competition between AA and 2-AG for these sites, combined with differential allosteric modulation, gives rise to a complex interplay between the substrates, leading to preferential oxygenation of AA. ..
  13. pmc Conservative Secondary Shell Substitution In Cyclooxygenase-2 Reduces Inhibition by Indomethacin Amides and Esters via Altered Enzyme Dynamics
    Mary E Konkle
    Departments of Biochemistry, Chemistry, and Pharmacology, Vanderbilt Institute of Chemical Biology, Center for Structural Biology, Center in Molecular Toxicology, Vanderbilt Ingram Cancer Center, Vanderbilt University School of Medicine, Nashville Tennessee 37232 0146, United States
    Biochemistry 55:348-59. 2016
    ..Kinetic analysis of inhibitor binding is consistent with the computational findings. ..
  14. pmc Cyclooxygenases: structural and functional insights
    Carol A Rouzer
    A B Hancock Jr Memorial Laboratory for Cancer Research, Department of Biochemistry, Vanderbilt Institute of Chemical Biology, Center in Molecular Toxicology, Vanderbilt University School of Medicine, Nashville, TN 37232 0146, USA
    J Lipid Res 50:S29-34. 2009
    ..However, recent discoveries call this paradigm into question and reveal as yet underappreciated functions for both enzymes. This review focuses on some of these new insights...
  15. pmc Protein modification by adenine propenal
    Sarah C Shuck
    A B Hancock Jr Memorial Laboratory for Cancer Research, Departments of Biochemistry, Chemistry, and Pharmacology, Mass Spectrometry Research Center, Center in Molecular Toxicology, Center for Structural Biology, Department of Medicine, Vanderbilt Institute of Chemical Biology, and Vanderbilt Ingram Cancer Center, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 0146, United States
    Chem Res Toxicol 27:1732-42. 2014
    ..Three sites of modification of XPA are in the DNA-binding domain, and two sites are subject to regulatory acetylation. Modification by adenine propenal dramatically reduces XPA's ability to bind to a DNA substrate. ..
  16. ncbi request reprint Mechanism of free radical oxygenation of polyunsaturated fatty acids by cyclooxygenases
    Carol A Rouzer
    A B Hancock Jr Memorial Laboratory for Cancer Research, Department of Biochemistry, Vanderbilt Institute of Chemical Biology, Vanderbilt Ingram Comprehensive Cancer Center, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 0146, USA
    Chem Rev 103:2239-304. 2003
  17. doi request reprint Green tea gets molecular
    Carol A Rouzer
    A B Hancock Jr Memorial Laboratory for Cancer Research, Department of Biochemistry, Vanderbilt Institute of Chemical Biology, Center in Molecular Toxicology, Vanderbilt Ingram Cancer Center, Vanderbilt University School of Medicine, Nashville, Tennessee, USA
    Cancer Prev Res (Phila) 4:1343-5. 2011
    ..The data provide a glimpse of the mechanism of action of EGCG and set a new bar for the future study of natural products with chemopreventive activity...
  18. ncbi request reprint Cyclooxygenase-1-dependent prostaglandin synthesis modulates tumor necrosis factor-alpha secretion in lipopolysaccharide-challenged murine resident peritoneal macrophages
    Carol A Rouzer
    Departments of Biochemistry and Chemistry, Vanderbilt Institute for Chemical Biology, Vanderbilt University School of Medicine, Nashville, TN 37232 0146
    J Biol Chem 279:34256-68. 2004
    ..These results demonstrate autocrine regulation of TNF-alpha secretion by endogenous PGs synthesized primarily by COX-1 in RPM and suggest that COX-1 may play a significant role in the regulation of the early response to endotoxemia...
  19. ncbi request reprint Kinetic and thermodynamic analysis of the hydrolytic ring-opening of the malondialdehyde-deoxyguanosine adduct, 3-(2'-deoxy-beta-D-erythro-pentofuranosyl)- pyrimido[1,2-alpha]purin-10(3H)-one
    James N Riggins
    A B Hancock Jr Memorial Laboratory for Cancer Research, Departments of Biochemistry and Chemistry, Vanderbilt Institute of Chemical Biology, Vanderbilt University School of Medicine, Nashville, TN 37232 0146, USA
    J Am Chem Soc 126:8237-43. 2004
    ..A mechanism is proposed for ring-opening of M1dG under basic conditions and a role is proposed for duplex DNA in accelerating the rate of ring-opening of M1dG at neutral pH...
  20. ncbi request reprint Isoform-selective interaction of cyclooxygenase-2 with indomethacin amides studied by real-time fluorescence, inhibition kinetics, and site-directed mutagenesis
    Sergei L Timofeevski
    Department of Biochemistry, Center in Molecular Toxicology, Vanderbilt Ingram Cancer Center, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA
    Biochemistry 41:9654-62. 2002
    ..The indomethacin conjugates described herein represent powerful tools for the investigation of a novel class of selective inhibitors of COX-2...
  21. ncbi request reprint Targeted cyclooxygenase gene (ptgs) exchange reveals discriminant isoform functionality
    Ying Yu
    Institute for Translational Medicine and Therapeutics, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    J Biol Chem 282:1498-506. 2007
    ..These mice will provide a valuable reagent with which to elucidate the distinct roles of the COX enzymes in mammalian biology...
  22. ncbi request reprint Desmethyl derivatives of indomethacin and sulindac as probes for cyclooxygenase-dependent biology
    Andrew S Felts
    ACS Chem Biol 2:479-83. 2007
    ..These compounds serve as useful probes of COX-dependent biology and may represent leads for antidiabetic and anticancer drugs...