Z Hong Zhou

Summary

Affiliation: University of California
Country: USA

Publications

  1. pmc Four levels of hierarchical organization, including noncovalent chainmail, brace the mature tumor herpesvirus capsid against pressurization
    Z Hong Zhou
    Department of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles UCLA, Los Angeles, CA 90095 7364, USA California NanoSystems Institute, UCLA, Los Angeles, CA 90095 7151, USA Department of Pathology and Laboratory Medicine, The University of Texas Medical School at Houston, Houston, TX 77030, USA Electronic address
    Structure 22:1385-98. 2014
  2. pmc Structures of viral membrane proteins by high-resolution cryoEM
    Z Hong Zhou
    Department of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles UCLA, Los Angeles, CA 90095 7364, USA California NanoSystems Institute, UCLA, Los Angeles, CA 90095 7227, USA Electronic address
    Curr Opin Virol 5:111-9. 2014
  3. pmc Towards atomic resolution structural determination by single-particle cryo-electron microscopy
    Z Hong Zhou
    Department of Microbiology, Immunology and Molecular Genetics and the California NanoSystems Institute, University of California at Los Angeles, 237 BSRB, 615 Charles E Young Dr S, Los Angeles, CA 90095 7364, USA
    Curr Opin Struct Biol 18:218-28. 2008
  4. pmc Atomic structure of human adenovirus by cryo-EM reveals interactions among protein networks
    Hongrong Liu
    Department of Microbiology, Immunology, and Molecular Genetics, University of California, Los Angeles UCLA, Los Angeles, CA 90095 7364, USA
    Science 329:1038-43. 2010
  5. pmc The smallest capsid protein mediates binding of the essential tegument protein pp150 to stabilize DNA-containing capsids in human cytomegalovirus
    Xinghong Dai
    Department of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, Los Angeles, California, USA
    PLoS Pathog 9:e1003525. 2013
  6. pmc Cryo-EM model of the bullet-shaped vesicular stomatitis virus
    Peng Ge
    Department of Microbiology, Immunology, and Molecular Genetics, University of California at Los Angeles UCLA, Los Angeles, CA 90095 7364, USA
    Science 327:689-93. 2010
  7. pmc Tetrahymena telomerase holoenzyme assembly, activation, and inhibition by domains of the p50 central hub
    Kyungah Hong
    Department of Molecular and Cell Biology, University of California, Berkeley, California, USA
    Mol Cell Biol 33:3962-71. 2013
  8. pmc Bluetongue virus coat protein VP2 contains sialic acid-binding domains, and VP5 resembles enveloped virus fusion proteins
    Xing Zhang
    Department of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, CA 90095 7364, USA
    Proc Natl Acad Sci U S A 107:6292-7. 2010
  9. pmc Biochemical and structural characterization of the capsid-bound tegument proteins of human cytomegalovirus
    Xuekui Yu
    Department of Microbiology, Immunology and Molecular Genetics, University of California at Los Angeles, USA
    J Struct Biol 174:451-60. 2011
  10. pmc Backbone model of an aquareovirus virion by cryo-electron microscopy and bioinformatics
    Lingpeng Cheng
    Department of Microbiology, Immunology and Molecular Genetics, University of California at Los Angeles, Los Angeles, CA 90095 7364, USA
    J Mol Biol 397:852-63. 2010

Research Grants

Collaborators

Detail Information

Publications78

  1. pmc Four levels of hierarchical organization, including noncovalent chainmail, brace the mature tumor herpesvirus capsid against pressurization
    Z Hong Zhou
    Department of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles UCLA, Los Angeles, CA 90095 7364, USA California NanoSystems Institute, UCLA, Los Angeles, CA 90095 7151, USA Department of Pathology and Laboratory Medicine, The University of Texas Medical School at Houston, Houston, TX 77030, USA Electronic address
    Structure 22:1385-98. 2014
    ..4) One hundred sixty-two belts concatenate to form noncovalent chainmail. The triplex heterotrimer orchestrates all four levels and likely drives maturation to an angular capsid that can withstand pressurization. ..
  2. pmc Structures of viral membrane proteins by high-resolution cryoEM
    Z Hong Zhou
    Department of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles UCLA, Los Angeles, CA 90095 7364, USA California NanoSystems Institute, UCLA, Los Angeles, CA 90095 7227, USA Electronic address
    Curr Opin Virol 5:111-9. 2014
    ....
  3. pmc Towards atomic resolution structural determination by single-particle cryo-electron microscopy
    Z Hong Zhou
    Department of Microbiology, Immunology and Molecular Genetics and the California NanoSystems Institute, University of California at Los Angeles, 237 BSRB, 615 Charles E Young Dr S, Los Angeles, CA 90095 7364, USA
    Curr Opin Struct Biol 18:218-28. 2008
    ....
  4. pmc Atomic structure of human adenovirus by cryo-EM reveals interactions among protein networks
    Hongrong Liu
    Department of Microbiology, Immunology, and Molecular Genetics, University of California, Los Angeles UCLA, Los Angeles, CA 90095 7364, USA
    Science 329:1038-43. 2010
    ..The third, mediated by IIIa and VIII, binds each GOS to five surrounding GONs. Optimization of adenovirus for cancer and gene therapy could target these networks...
  5. pmc The smallest capsid protein mediates binding of the essential tegument protein pp150 to stabilize DNA-containing capsids in human cytomegalovirus
    Xinghong Dai
    Department of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, Los Angeles, California, USA
    PLoS Pathog 9:e1003525. 2013
    ..Together, our structural and biochemical results point to a mechanism whereby SCP recruits pp150 to stabilize genome-containing capsid for the production of infectious HCMV virion...
  6. pmc Cryo-EM model of the bullet-shaped vesicular stomatitis virus
    Peng Ge
    Department of Microbiology, Immunology, and Molecular Genetics, University of California at Los Angeles UCLA, Los Angeles, CA 90095 7364, USA
    Science 327:689-93. 2010
    ..Together, our data suggest a mechanism of VSV assembly in which the nucleocapsid spirals from the tip to become the helical trunk, both subsequently framed and rigidified by the M layer...
  7. pmc Tetrahymena telomerase holoenzyme assembly, activation, and inhibition by domains of the p50 central hub
    Kyungah Hong
    Department of Molecular and Cell Biology, University of California, Berkeley, California, USA
    Mol Cell Biol 33:3962-71. 2013
    ..Also, in EM structures of holoenzymes, only the N-terminal domain of p50 is visible. Our findings provide new insights about subunit and domain interactions and functions within the Tetrahymena telomerase holoenzyme. ..
  8. pmc Bluetongue virus coat protein VP2 contains sialic acid-binding domains, and VP5 resembles enveloped virus fusion proteins
    Xing Zhang
    Department of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, CA 90095 7364, USA
    Proc Natl Acad Sci U S A 107:6292-7. 2010
    ....
  9. pmc Biochemical and structural characterization of the capsid-bound tegument proteins of human cytomegalovirus
    Xuekui Yu
    Department of Microbiology, Immunology and Molecular Genetics, University of California at Los Angeles, USA
    J Struct Biol 174:451-60. 2011
    ....
  10. pmc Backbone model of an aquareovirus virion by cryo-electron microscopy and bioinformatics
    Lingpeng Cheng
    Department of Microbiology, Immunology and Molecular Genetics, University of California at Los Angeles, Los Angeles, CA 90095 7364, USA
    J Mol Biol 397:852-63. 2010
    ..Overall, this first backbone model of an aquareovirus virion provides a wealth of structural information for understanding the structural basis of GCRV assembly and transcription...
  11. ncbi request reprint Architecture of the herpes simplex virus major capsid protein derived from structural bioinformatics
    Matthew L Baker
    National Center for Macromolecular Imaging, Baylor College of Medicine, Houston, TX 77030, USA
    J Mol Biol 331:447-56. 2003
    ..The combined structural and sequence-based informatics has led to an architectural model of VP5. This model placed in the context of the capsid provides insights into the strategies used to achieve viral capsid stability...
  12. pmc Unique structures in a tumor herpesvirus revealed by cryo-electron tomography and microscopy
    Wei Dai
    Department of Pathology and Laboratory Medicine, University of Texas Medical School at Houston, Houston, TX 77030, USA
    J Struct Biol 161:428-38. 2008
    ....
  13. ncbi request reprint Polypeptide-Based Gold Nanoshells for Photothermal Therapy
    Kristine M Mayle
    Department of Bioengineering, University of California, Los Angeles, CA, USA
    J Lab Autom . 2016
    ..To our knowledge, our results represent the first ever use of block copolypeptide vesicles as the core material of gold nanoshells...
  14. pmc Murine gammaherpesvirus 68 ORF52 encodes a tegument protein required for virion morphogenesis in the cytoplasm
    Eric Bortz
    Molecular Biology IDP, University of California at Los Angeles, Los Angeles, CA 90095, USA
    J Virol 81:10137-50. 2007
    ..Thus, ORF52 is essential for the tegumentation and egress of infectious MHV-68 particles in the cytoplasm, suggesting an important conserved function in gammaherpesvirus virion morphogenesis...
  15. doi request reprint Engineering polypeptide coatings to augment gene transduction and in vivo stability of adenoviruses
    Ziyue Karen Jiang
    Molecular and Medical Pharmacology, University of California, Los Angeles, Los Angeles, CA 90095, USA
    J Control Release 166:75-85. 2013
    ..Advantages such as facile coating of the Ad vector, design flexibility and ease of attaching ligands to the polypeptides make this system potentially useful as a platform for adding functionalities to Ad to target cancer metastasis...
  16. pmc Cryo-electron tomography of Kaposi's sarcoma-associated herpesvirus capsids reveals dynamic scaffolding structures essential to capsid assembly and maturation
    Binbin Deng
    Department of Pathology and Laboratory Medicine and The Keck Center Pharmacoinformatics Training Program, The University of Texas Medical School at Houston, Houston, TX 77030, USA
    J Struct Biol 161:419-27. 2008
    ..This information, taken together with previous observations, has allowed us to propose a detailed pathway of herpesvirus capsid assembly and maturation...
  17. pmc Dissecting human cytomegalovirus gene function and capsid maturation by ribozyme targeting and electron cryomicroscopy
    Xuekui Yu
    Division of Infectious Diseases, School of Public Health, University of California, Berkeley, CA 94720, USA
    Proc Natl Acad Sci U S A 102:7103-8. 2005
    ..These results therefore provide key insights into an essential step of HCMV infection using an RNase P ribozyme-based inhibition strategy...
  18. pmc Three-dimensional localization of the smallest capsid protein in the human cytomegalovirus capsid
    Xuekui Yu
    Department of Pathology and Laboratory Medicine, University of Texas Medical School at Houston, 6431 Fannin St, MSB 2 280, Houston, TX 77030, USA
    J Virol 79:1327-32. 2005
    ....
  19. pmc Electron tomography reveals polyhedrin binding and existence of both empty and full cytoplasmic polyhedrosis virus particles inside infectious polyhedra
    Justin Chen
    Department of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, Los Angeles, CA 90095, USA
    J Virol 85:6077-81. 2011
    ..Furthermore, both full (with RNA) and empty (no RNA) capsids were found inside polyhedra, suggesting a spontaneous RNA encapsidating process for CPV assembly in vivo...
  20. pmc Cryo-EM structure of the mature dengue virus at 3.5-Å resolution
    Xiaokang Zhang
    Department of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles UCLA, Los Angeles, California, USA
    Nat Struct Mol Biol 20:105-10. 2013
    ..Thus, M serves as a multistep chaperone of E to control the conformational changes accompanying maturation and infection. These pH-sensitive interactions could serve as targets for drug discovery...
  21. pmc Access to RNA encapsidated in the nucleocapsid of vesicular stomatitis virus
    Todd J Green
    Department of Microbiology, University of Alabama at Birmingham, 1025 18th Street South, Birmingham, AL 35294, USA
    J Virol 85:2714-22. 2011
    ..Polyribonucleotides were reencapsidated into the empty NLP, and the crystal structures were determined. The crystal structures revealed variable degrees of association of the N protein with a specific RNA sequence...
  22. pmc Protein interactions in the murine cytomegalovirus capsid revealed by cryoEM
    Wong H Hui
    The California NanoSystems Institute CNSI, University of California, Los Angeles UCLA, Los Angeles, CA, 90095, USA
    Protein Cell 4:833-45. 2013
    ....
  23. pmc Atomic model of CPV reveals the mechanism used by this single-shelled virus to economically carry out functions conserved in multishelled reoviruses
    Xuekui Yu
    Department of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, Los Angeles, CA 90095 7364, USA
    Structure 19:652-61. 2011
    ..TP is also simplified, containing only domains involved in RNA capping. Our results illustrate how CPV proteins have evolved in a coordinative manner to economically carry out their conserved functions...
  24. pmc 3.3 A cryo-EM structure of a nonenveloped virus reveals a priming mechanism for cell entry
    Xing Zhang
    Department of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, Los Angeles, CA 90095 7364, USA
    Cell 141:472-82. 2010
    ..These results suggest a well-orchestrated process of nonenveloped virus entry involving autocleavage of the penetration protein prior to exposure of its membrane-insertion finger...
  25. pmc Subnanometer-resolution structures of the grass carp reovirus core and virion
    Lingpeng Cheng
    Department of Microbiology, Immunology and Molecular Genetics, The University of California at Los Angeles, CA 90095 7364, USA
    J Mol Biol 382:213-22. 2008
    ....
  26. ncbi request reprint A 9 angstroms single particle reconstruction from CCD captured images on a 200 kV electron cryomicroscope
    Christopher R Booth
    Program in Structural and Computational Biology and Molecular Biophysics, Baylor College of Medicine, Houston, TX 77030, USA
    J Struct Biol 147:116-27. 2004
    ....
  27. pmc Structures of the human pyruvate dehydrogenase complex cores: a highly conserved catalytic center with flexible N-terminal domains
    Xuekui Yu
    Department of Pathology and Laboratory Medicine, The University of Texas Medical School at Houston, Houston, TX 77030, USA
    Structure 16:104-14. 2008
    ....
  28. ncbi request reprint Cryo-electron microscopy and three-dimensional reconstructions of hepatitis C virus particles
    Xuekui Yu
    Department of Pathology and Laboratory Medicine, The University of Texas Medical School at Houston, Houston, TX 77030, USA
    Virology 367:126-34. 2007
    ..This first 3D structural analysis of HCV particles offers important insights into the elusive mechanisms of HCV assembly and maturation...
  29. ncbi request reprint Fast rotational matching of single-particle images
    Yao Cong
    School of Health Information Sciences and Institute of Molecular Medicine, University of Texas Health Science Center at Houston, 7000 Fannin St, Suite 600, Houston, TX 77030, USA
    J Struct Biol 152:104-12. 2005
    ..Moreover, at sub-nanometer resolution, GroEL and rice dwarf virus reconstructions exhibit a remarkable resolution gain of 10-20% that is attributed to the novel image alignment kernel...
  30. pmc Three-dimensional structures of the A, B, and C capsids of rhesus monkey rhadinovirus: insights into gammaherpesvirus capsid assembly, maturation, and DNA packaging
    Xue Kui Yu
    Department of PathologyLaboratory Medicine, University of Texas at Houston Medical School, Houston, Texas 77030, USA
    J Virol 77:13182-93. 2003
    ..This structural disparity suggests that SCIP and the triplex, together with tegument and envelope proteins, confer structural and potentially functional specificities to alpha-, beta-, and gammaherpesviruses...
  31. pmc Cellular architecture of Treponema pallidum: novel flagellum, periplasmic cone, and cell envelope as revealed by cryo electron tomography
    Jun Liu
    Department of Pathology and Laboratory Medicine, University of Texas Medical School at Houston, 6431 Fannin, MSB 2 228, Houston, TX 77030, USA
    J Mol Biol 403:546-61. 2010
    ..Our findings provide the most detailed structural understanding of periplasmic flagella and the surrounding cell envelope, which enable this enigmatic bacterium to efficiently penetrate tissue and to escape host immune responses...
  32. ncbi request reprint Conformational flexibility of pyruvate dehydrogenase complexes: a computational analysis by quantized elastic deformational model
    Yifei Kong
    Graduate Program of Structural and Computational Biology, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA
    J Mol Biol 330:129-35. 2003
    ....
  33. pmc Three-dimensional visualization of gammaherpesvirus life cycle in host cells by electron tomography
    Li Peng
    Department of Microbiology, Immunology, and Molecular Genetics, University of California, Los Angeles, Los Angeles, CA 90095, USA
    Structure 18:47-58. 2010
    ....
  34. doi request reprint Structure and Conductivity of Semiconducting Polymer Hydrogels
    Rachel C Huber
    Department of Chemistry and Biochemistry, UCLA, Los Angeles, California 90095 1569, United States
    J Phys Chem B 120:6215-24. 2016
    ..Finally, time-resolved microwave conductivity measurements on dried samples show an increase in carrier mobility after THF annealing of the PFT gel, likely due to increased connectivity within the polymer network. ..
  35. pmc Direct visualization of the putative portal in the Kaposi's sarcoma-associated herpesvirus capsid by cryoelectron tomography
    Binbin Deng
    Department of Pathology and Laboratory Medicine, University of Texas Medical School at Houston, 6431 Fannin St, MSB 2 280, Houston, TX 77030, USA
    J Virol 81:3640-4. 2007
    ..This putative KSHV portal is an internally localized, umbilicated structure and lacks all of the external machineries characteristic of portals in DNA bacteriophages...
  36. pmc A new topology of the HK97-like fold revealed in Bordetella bacteriophage by cryoEM at 3.5 A resolution
    Xing Zhang
    California NanoSystems Institute, University of California, Los Angeles, Los Angeles, United States
    elife 2:e01299. 2013
    ..Despite these complex interactions, the termini of both CP and MCP are ideally positioned for DGR-based phage-display engineering. DOI: http://dx.doi.org/10.7554/eLife.01299.001. ..
  37. pmc Fab crystallization and preliminary X-ray analysis of NC-1, an anti-HIV-1 antibody that recognizes the six-helix bundle core of gp41
    Lei Jin
    Department of Microbiology, Immunology and Molecular Genetics, David Geffen School of Medicine, University of California, Los Angeles, CA 90095, USA
    Acta Crystallogr Sect F Struct Biol Cryst Commun 66:854-7. 2010
    ..0 A. There is one Fab molecule in the asymmetric unit, with 67.5% solvent content. An X-ray diffraction data set was collected at 3.2 A resolution and a clear molecular-replacement solution was obtained for solution of the structure...
  38. pmc 3.5Å cryoEM structure of hepatitis B virus core assembled from full-length core protein
    Xuekui Yu
    Department of Microbiology, Immunology and Molecular Genetics, University of California Los Angeles, Los Angeles, California, United States of America California NanoSystems Institute, University of California Los Angeles, Los Angeles, California, United States of America
    PLoS ONE 8:e69729. 2013
    ..Our data supports a role of ARD in mediating communication between inside and outside of the core during HBV maturation and envelopment. ..
  39. pmc Single particle electron microscopy analysis of the bovine anion exchanger 1 reveals a flexible linker connecting the cytoplasmic and membrane domains
    Jiansen Jiang
    Department of Microbiology, Immunology and Molecular Genetics, and California Nanosystems Institute, University of California Los Angeles, Los Angeles, California, United States of America
    PLoS ONE 8:e55408. 2013
    ..Image classification demonstrated substantial flexibility in the linker region. We propose a mechanism whereby flexibility of the linker region plays a critical role in regulating red cell elasticity...
  40. ncbi request reprint Quantitative imaging of single, unstained viruses with coherent x rays
    Changyong Song
    Department of Physics and Astronomy, University of California, Los Angeles, California 90095, USA
    Phys Rev Lett 101:158101. 2008
    ..Moreover, our experiment is directly transferable to the use of x-ray free electron lasers, and represents an experimental milestone towards the x-ray imaging of large protein complexes...
  41. pmc The architecture of Tetrahymena telomerase holoenzyme
    Jiansen Jiang
    Department of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, California 90095, USA
    Nature 496:187-92. 2013
    ..These studies provide the first view of the extensive network of subunit associations necessary for telomerase holoenzyme assembly and physiological function...
  42. pmc Hydrogen-bonding networks and RNA bases revealed by cryo electron microscopy suggest a triggering mechanism for calcium switches
    Peng Ge
    Department of Microbiology, Immunology, and Molecular Genetics and the California NanoSystems Institute, University of California, Los Angeles, CA 90095 7364, USA
    Proc Natl Acad Sci U S A 108:9637-42. 2011
    ..In turn, the coat protein-RNA interactions in our structure favor an adenine-X-guanine (A*G) motif over the G*A motif of the dormant state, thus offering an explanation underlying viral assembly initiation by an AAG motif...
  43. pmc Model of the trimeric fiber and its interactions with the pentameric penton base of human adenovirus by cryo-electron microscopy
    Hongrong Liu
    Department of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, Los Angeles, CA 90095 7364, USA
    J Mol Biol 406:764-74. 2011
    ..These interaction sites provide the molecular basis for the symmetry mismatch and can be targeted for optimizing adenovirus for gene therapy applications...
  44. pmc Structure of Tetrahymena telomerase reveals previously unknown subunits, functions, and interactions
    Jiansen Jiang
    Department of Chemistry and Biochemistry, University of California, Los Angeles UCLA, Los Angeles, CA 90095, USA Department of Microbiology, Immunology, and Molecular Genetics, UCLA, Los Angeles, CA 90095, USA California NanoSystems Institute, UCLA, Los Angeles, CA 90095, USA
    Science 350:aab4070. 2015
    ..Our findings provide structural and mechanistic insights into telomerase holoenzyme function. ..
  45. pmc CryoEM and mutagenesis reveal that the smallest capsid protein cements and stabilizes Kaposi's sarcoma-associated herpesvirus capsid
    Xinghong Dai
    Department of Microbiology, Immunology and Molecular Genetics, The California NanoSystems Institute, Bioengineering Program, and
    Proc Natl Acad Sci U S A 112:E649-56. 2015
    ..These data support a mechanistic model that tumor herpesvirus SCP reinforces the capsid for genome packaging, thus acting as a cementing protein similar to those found in many bacteriophages...
  46. ncbi request reprint Web portal to an image database for high-resolution three-dimensional reconstruction
    Wei Dai
    School of Health Information Sciences, University of Texas Health Science Center at Houston, 7000 Fannin, Houston, TX 77030, USA
    J Struct Biol 144:238-45. 2003
    ..Our examples should thus provide general guidelines applicable to other cryoEM technology development efforts...
  47. pmc Identification of proteins associated with murine gammaherpesvirus 68 virions
    Eric Bortz
    Molecular Biology Institute, University of California at Los Angeles, Los Angeles, California 90095, USA
    J Virol 77:13425-32. 2003
    ....
  48. pmc 3.88 A structure of cytoplasmic polyhedrosis virus by cryo-electron microscopy
    Xuekui Yu
    Department of Pathology and Laboratory Medicine, The University of Texas Medical School at Houston, Houston, Texas 77030, USA
    Nature 453:415-9. 2008
    ..We have also discovered a messenger RNA release hole coupled with the mRNA capping machinery unique to CPV. Furthermore, we have identified the polyhedrin-binding domain, a structure that has potential in nanobiotechnology applications...
  49. pmc Structure of the core editing complex (L-complex) involved in uridine insertion/deletion RNA editing in trypanosomatid mitochondria
    Feng Li
    Department of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, Los Angeles, CA 90095, USA
    Proc Natl Acad Sci U S A 106:12306-10. 2009
    ..The REL1 ligase was localized on the model to the base of the apex by decoration with REL1-specific IgG. This structure should prove useful for a detailed analysis of the editing reaction...
  50. doi request reprint CryoEM structure of the Methanospirillum hungatei archaellum reveals structural features distinct from the bacterial flagellum and type IV pili
    Nicole Poweleit
    Department of Microbiology, Immunology, and Molecular Genetics, University of California, Los Angeles UCLA, Los Angeles, California 90095, USA
    Nat Microbiol 2:16222. 2016
    ..These extensive inter-subunit interactions and the absence of a central pore in the archaellum distinguish it from both the bacterial flagellum and type IV pili...
  51. doi request reprint Atomic model of a nonenveloped virus reveals pH sensors for a coordinated process of cell entry
    Xing Zhang
    California NanoSystems Institute, University of California, Los Angeles UCLA, Los Angeles, California, USA
    Nat Struct Mol Biol 23:74-80. 2016
    ..Exposing BTV to low pH detaches VP2 and dramatically refolds the dagger and unfurling domains of VP5. Our biochemical and structure-guided-mutagenesis studies support these coordinated pH-sensing mechanisms. ..
  52. pmc A putative ATPase mediates RNA transcription and capping in a dsRNA virus
    Xuekui Yu
    Department of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, Los Angeles, United States
    elife 4:e07901. 2015
    ..Taken together, our data support a role of the putative ATPase in mediating the activation of mRNA transcription and capping within the confines of the virus. ..
  53. pmc In situ structures of the segmented genome and RNA polymerase complex inside a dsRNA virus
    Xing Zhang
    California NanoSystems Institute, University of California, Los Angeles, California 90095, USA
    Nature 527:531-4. 2015
    ..These findings establish the link between sensing of environmental cues by the external proteins and activation of endogenous RNA transcription by the TEC inside the virus. ..
  54. pmc Atomic structures of a bactericidal contractile nanotube in its pre- and postcontraction states
    Peng Ge
    1 Department of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles UCLA, Los Angeles, California, USA 2 California NanoSystems Institute CNSI, UCLA, Los Angeles, California, USA
    Nat Struct Mol Biol 22:377-82. 2015
    ..We propose that the contraction process draws energy from electrostatic and shape complementarities to insert the inner tube through bacterial cell membranes to eventually kill the bacteria. ..
  55. pmc Influenza virus morphogenesis and budding
    Debi P Nayak
    Department of Microbiology, Immunology and Molecular Genetics, Molecular Biology Institute, Jonsson Comprehensive Cancer Center, David Geffen School of Medicine, University of California at Los Angeles, Los Angeles, CA 90095, USA
    Virus Res 143:147-61. 2009
    ..We provide our current understanding of these important processes leading to the production of infectious influenza virus particles...
  56. pmc Polyribosomes are molecular 3D nanoprinters that orchestrate the assembly of vault particles
    Jan Mrazek
    Department of Biological Chemistry, David Geffen School of Medicine, Department of Microbiology, Immunology and Molecular Genetics, and California Nanosystems Institute, University of California at Los Angeles, Los Angeles, California 90095, United States
    ACS Nano 8:11552-9. 2014
    ....
  57. doi request reprint Mesoporous metal and metal alloy particles synthesized by aerosol-assisted confined growth of nanocrystals
    Qiangfeng Xiao
    Department of Chemical and Biomolecular Engineering, University of California, Los Angeles, CA 90095, USA
    Angew Chem Int Ed Engl 51:10546-50. 2012
    ..The confined growth of nanocrystals in aerosol droplets leads to the formation of these particles with defined composition...
  58. ncbi request reprint Reversible aggregation of mouse prion protein derivatives with PrPSC-like structural properties
    Bao Yuan Lu
    Research Center for Protein Chemistry, Institute of Molecular Medicine, Department of Biochemistry and Molecular Biology, The University of Texas, Houston, Texas 77030, USA
    J Protein Chem 22:115-26. 2003
    ..The properties of reversible aggregation of mPrP-a, mPrP-b, and mPrP-c bear a striking resemblance to that observed with aggregates of hamster PrPSC...
  59. pmc 3D electron microscopy reveals the variable deposition and protein dynamics of the peripheral pyruvate dehydrogenase component about the core
    Yingqi Gu
    Department of Pathology and Laboratory Medicine, University of Texas Medical School, Houston, TX 77030, USA
    Proc Natl Acad Sci U S A 100:7015-20. 2003
    ..The flexibility of the complex may enhance the catalytic proficiency of this macromolecular machine by promoting the channeling of the intermediates of catalysis between the active sites...
  60. ncbi request reprint Determination of icosahedral virus structures by electron cryomicroscopy at subnanometer resolution
    Z Hong Zhou
    Department of Pathology and Laboratory Medicine, University of Texas Houston Medical School, Houston, Texas 77030, USA
    Adv Protein Chem 64:93-124. 2003
  61. pmc Atomic resolution cryo electron microscopy of macromolecular complexes
    Z Hong Zhou
    Department of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles UCLA, Los Angeles, California, USA
    Adv Protein Chem Struct Biol 82:1-35. 2011
    ....
  62. pmc Organization of capsid-associated tegument components in Kaposi's sarcoma-associated herpesvirus
    Xinghong Dai
    Department of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles UCLA, Los Angeles, California, USA The California NanoSystems Institute, University of California, Los Angeles UCLA, Los Angeles, California, USA Biomedical Engineering Interdepartmental Program, University of California, Los Angeles UCLA, Los Angeles, California, USA
    J Virol 88:12694-702. 2014
    ..This newly discovered organization of triplex-ORF32-ORF19 also resolves a long-standing mystery surrounding the virion location and conformation of alphaherpesvirus UL25 protein...
  63. pmc Three-dimensional structure of tropism-switching Bordetella bacteriophage
    Wei Dai
    Department of Pathology and Laboratory Medicine, University of Texas Medical School, Houston, TX 77030, USA
    Proc Natl Acad Sci U S A 107:4347-52. 2010
    ..The localization of Mtd at the distal ends of the six tail fibers explains the high avidity binding of Mtd molecules to cell surfaces for initiation of infection...
  64. pmc Correcting for the ewald sphere in high-resolution single-particle reconstructions
    Peter A Leong
    Department of Applied Physics, California Institute of Technology, Pasadena, California, USA
    Methods Enzymol 482:369-80. 2010
    ..Here, we review the basic problem and our implementation of the "paraboloid" reconstruction method, which overcomes the limitation by averaging information from images recorded from different points of view...
  65. pmc Mini-chromosome maintenance complexes form a filament to remodel DNA structure and topology
    Ian M Slaymaker
    Molecular and Computational Biology, University of Southern California, Los Angeles, CA 90089, USA
    Nucleic Acids Res 41:3446-56. 2013
    ..Finally, using yeast genetics, we show that the inter-subunit interactions, important for MCM filament formation, play a role for cell growth and survival...
  66. pmc Conserved SMP domains of the ERMES complex bind phospholipids and mediate tether assembly
    Andrew P AhYoung
    Department of Biological Chemistry, David Geffen School of Medicine, University of California, Los Angeles, CA 90095
    Proc Natl Acad Sci U S A 112:E3179-88. 2015
    ..Our findings highlight roles for SMP domains in ERMES assembly and phospholipid binding and suggest a structure-based mechanism for the facilitated transport of phospholipids between organelles...
  67. pmc Structural, mass and elemental analyses of storage granules in methanogenic archaeal cells
    Daniel B Toso
    Department of Microbiology, Immunology, and Molecular Genetics Electron Imaging Center for Nanomachines, California NanoSystems Institute, Los Angeles, CA 90095, USA
    Environ Microbiol 13:2587-99. 2011
    ..This positioning ability points to a genetic program that spatially and temporally directs the accumulation of polyphosphate into a storage granule, perhaps for energy-consuming activities, such as cell maintenance, division or motility...
  68. pmc Three-dimensional structure of the Trypanosome flagellum suggests that the paraflagellar rod functions as a biomechanical spring
    Louise C Hughes
    Department of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, Los Angeles, California, United States of America
    PLoS ONE 7:e25700. 2012
    ..These findings provide insight into the structural foundations that underlie the distinctive flagellar waveform that is a hallmark of T. brucei cell motility...
  69. pmc AlphaB-crystallin is found in detergent-resistant membrane microdomains and is secreted via exosomes from human retinal pigment epithelial cells
    Rajendra K Gangalum
    Jules Stein Eye Institute, UCLA, Los Angeles, California 90095, USA
    J Biol Chem 286:3261-9. 2011
    ....
  70. ncbi request reprint Deriving folds of macromolecular complexes through electron cryomicroscopy and bioinformatics approaches
    Wah Chiu
    Program in Structural and Computational Biology and Molecular Biophysics, Baylor College of Medicine, Houston, Texas 77030, USA
    Curr Opin Struct Biol 12:263-9. 2002
    ..Such approaches have been demonstrated with the 6.8 A structure of the rice dwarf virus to derive models for the major capsid shell proteins...
  71. ncbi request reprint Cytoplasmic polyhedrosis virus structure at 8 A by electron cryomicroscopy: structural basis of capsid stability and mRNA processing regulation
    Z Hong Zhou
    Department of Pathology and Laboratory Medicine, University of Texas Medical School, Houston, TX 77030, USA
    Structure 11:651-63. 2003
    ....
  72. pmc Limiting factors in atomic resolution cryo electron microscopy: no simple tricks
    Xing Zhang
    Department of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, 237 BSRB, 615 Charles E Young Dr S, Los Angeles, CA 90095 7364, USA
    J Struct Biol 175:253-63. 2011
    ..These analyses and our proposed strategies offer useful guidance for future experimental designs targeting atomic resolution cryoEM reconstruction...
  73. pmc DGCR8 recognizes primary transcripts of microRNAs through highly cooperative binding and formation of higher-order structures
    Michael Faller
    Department of Biological Chemistry, David Geffen School of Medicine, University of California at Los Angeles, Los Angeles, CA 90095, USA
    RNA 16:1570-83. 2010
    ..Our study provides a molecular basis for recognition of pri-miRNAs by DGCR8. We further propose that the higher-order structures of the DGCR8-pri-miRNA complexes trigger the cleavage of pri-miRNAs by Drosha...
  74. pmc Chaperone fusion proteins aid entropy-driven maturation of class II viral fusion proteins
    Peng Ge
    Department of Microbiology, Immunology, and Molecular Genetics, University of California Los Angeles UCLA, Los Angeles, CA 90095 7364, USA California NanoSystems Institute, UCLA, Los Angeles, CA 90095 7227, USA
    Trends Microbiol 22:100-6. 2014
    ..Drastic conformation changes and reorganization of these viral membrane proteins occur during the transition from their metastable to stable structural states in a unidirectional, entropy-driven process. ..
  75. ncbi request reprint Symmetry-adapted spherical harmonics method for high-resolution 3D single-particle reconstructions
    Hongrong Liu
    Institute of Modern Physics, Xiangtan University, Xiangtan, Hunan 411105, People s Republic of China
    J Struct Biol 161:64-73. 2008
    ..three decades ago. Our 3D reconstruction method can be extended to macromolecular complexes with other symmetry types and is thus likely to impact future high-resolution cryoEM single-particle reconstruction efforts in general...
  76. ncbi request reprint IMIRS: a high-resolution 3D reconstruction package integrated with a relational image database
    Yuyao Liang
    Department of Pathology and Laboratory Medicine, University of Texas Houston Medical School, 77030, USA
    J Struct Biol 137:292-304. 2002
    ..We demonstrate the applicability of IMIRS in icosahedral reconstruction toward high resolution by using it to obtain an 8-A 3D structure of an intermediate-sized dsRNA virus...
  77. ncbi request reprint Molecular interactions and viral stability revealed by structural analyses of chemically treated cypovirus capsids
    Hong Zhang
    Department of Pathology and Laboratory Medicine, University of Texas Houston Medical School, 77030, USA
    Virology 298:45-52. 2002
    ..The observed structural changes associated with the removal of the turret suggest critical structural roles of the turret in maintaining capsid integrity in addition to its enzymatic activities...
  78. pmc Three-dimensional localization of pORF65 in Kaposi's sarcoma-associated herpesvirus capsid
    Pierrette Lo
    Department of Pathology and Laboratory Medicine, University of Texas Houston Medical School, Houston, Texas 77030, USA
    J Virol 77:4291-7. 2003
    ..The location of SCP at the outermost regions of the capsid suggests a possible role in mediating capsid interactions with the tegument and cytoskeletal proteins during infection...

Research Grants2

  1. STRUCTURAL STUDIES OF HUMAN CYTOMEGALOVIRUS
    Z Zhou; Fiscal Year: 2004
    ..abstract_text> ..
  2. Structure and Assembly of KSHV Capsids and Virions
    Z Zhou; Fiscal Year: 2006
    ..The results of these experiments will lead to a better understanding of the process of KSHV lytic replication and the identification of potential targets for therapeutic intervention. ..