Affiliation: University of California
- Cell-free recombination of immunoglobulin switch-region DNA with nuclear extractsK Zhang
The Hart and Louise Lyon Laboratory, UCLA School of Medicine, Los Angeles, California 90095 1680, USA
Clin Immunol 94:140-51. 2000..This in vitro system for Ig switch-region DNA recombination using cell-free nuclear extracts will permit the dissection of the events involved in IgE class switch recombination, a critical event in the development of allergic diseases...
- Chimeric human fcgamma-allergen fusion proteins in the prevention of allergyKe Zhang
The Hart and Louise Lyon Laboratory, Division of Clinical Immunology Allergy, Department of Medicine, UCLA School of Medicine, 52 175 CHS, 10833 Le Conte Avenue, Los Angeles, CA 90095 1680, USA
Immunol Allergy Clin North Am 27:93-103. 2007..This article summarizes the therapeutic features and potential of this novel fusion protein for allergic IT...
- Accessibility control and machinery of immunoglobulin class switch recombinationKe Zhang
The Hart and Louse Lyon Laboratory, Division of Clinical Immunology Allergy, Department of Medicine, University of California Los Angeles, School of Medicine, 90095 1680, USA
J Leukoc Biol 73:323-32. 2003..CSR generates a new transcriptional unit for production of a class-switched Ig isotype...
- Inhibition of allergen-specific IgE reactivity by a human Ig Fcgamma-Fcepsilon bifunctional fusion proteinKe Zhang
Hart and Louis Lyon Laboratory, Division of Clinical Immunology and Allergy, Department of Medicine, University of California Los Angeles School of Medicine, CA 90095 1680, USA
J Allergy Clin Immunol 114:321-7. 2004..We have previously shown that an Fcgamma-Fcepsilon fusion protein, human IgG-IgE Fc fusion protein (GE2), could inhibit FcepsilonRI-mediated mediator releases in vitro and in vivo...
- Inhibition of interleukin-4-induced class switch recombination by a human immunoglobulin Fc gamma-Fc epsilon chimeric proteinTakechiyo Yamada
Hart and Louis Laboratory, Division of Clinical Immunology, Department of Medicine, UCLA School of Medicine, California 90095 1680, USA
J Biol Chem 278:32818-24. 2003....
- A novel human immunoglobulin Fc gamma Fc epsilon bifunctional fusion protein inhibits Fc epsilon RI-mediated degranulationDaocheng Zhu
The Hart and Louise Lyon Laboratory, Division of Clinical Immunology Allergy, Department of Medicine, University of California Los Angeles School of Medicine, Los Angeles, California, USA
Nat Med 8:518-21. 2002..This approach, using a Fc gamma Fc epsilon fusion protein to co-aggregate Fc epsilon RI with a receptor containing an immunoreceptor tyrosine-based inhibition motif, has therapeutic potential in IgE- and Fc epsilon RI-mediated diseases...
- "Accentuate the negative, eliminate the positive": engineering allergy therapeutics to block allergic reactivity through negative signalingAndrew Saxon
Department of Medicine, University of California, Los Angeles, CA 90095 16908, USA
J Allergy Clin Immunol 121:320-5. 2008..Fcgamma-Fel d 1 blocked human mast cell Fel d 1-induced allergic reactivity in vitro and in vivo in murine models while functioning as an immunogen but not as an allergen...
- Genetically engineered negative signaling molecules in the immunomodulation of allergic diseasesAndrew Saxon
The Hart and Louise Lyon Laboratory, Division of Clinical Immunology Allergy, Department of Medicine, David Geffen School of Medicine at UCLA, Los Angeles, CA 90095 1680, USA
Curr Opin Allergy Clin Immunol 4:563-8. 2004....
- A chimeric human-cat fusion protein blocks cat-induced allergyDaocheng Zhu
The Hart and Louise Lyon Laboratory, Division of Clinical Immunology Allergy, Department of Medicine, UCLA School of Medicine, 10833 Le Conte Avenue, Los Angeles, California 90095 1680, USA
Nat Med 11:446-9. 2005..The Fcgamma-Fel d1 protein alone did not induce mediator release. Chimeric human Fcgamma-allergen fusion proteins may provide a new therapeutic platform for the immune-based therapy of allergic disease...
- Modifications to an Fcgamma-Fcvarepsilon fusion protein alter its effectiveness in the inhibition of FcvarepsilonRI-mediated functionsLisa Chan Allen
Hart and Louise Lyon Laboratory, Division of Clinical Immunology Allergy, David Geffen School of Medicine, University of California, Los Angeles, CA 90095, USA
J Allergy Clin Immunol 120:462-8. 2007..GE2, a human bifunctional Fcgamma-Fcvarepsilon fusion protein cross-links FcgammaRIIb and FcvarepsilonRI on human mast cells and basophils and results in inhibition of FcvarepsilonRI-mediated functions...
- CD40-mediated p38 mitogen-activated protein kinase activation is required for immunoglobulin class switch recombination to IgEKe Zhang
Hart and Louis Laboratory, Division of Clinical Immunology, Department of Medicine, UCLA School of Medicine, 90095, USA
J Allergy Clin Immunol 110:421-8. 2002..The specific pathways activated by CD40 signaling involving CD40-dependent Ig class switch recombination (CSR) have not been defined...
- CD45 controls interleukin-4-mediated IgE class switch recombination in human B cells through its function as a Janus kinase phosphataseTakechiyo Yamada
Hart and Louis Laboratory, Division of Clinical Immunology, Department of Medicine, UCLA School of Medicine, Los Angeles, California 90095 1680, USA
J Biol Chem 277:28830-5. 2002..CD45 may be an appropriate target drug for modulating IgE in allergic diseases...
- Efficiency of Iepsilon promoter-directed switch recombination in GFP expression-based switch constructs works synergistically with other promoter and/or enhancer elements but is not tightly linked to the strength of transcriptionKe Zhang
Division of Clinical Immunology, Department of Medicine, UCLA School of Medicine, Los Angeles, USA
Eur J Immunol 32:424-34. 2002..Our results suggest that the mechanism(s) underlying the transcriptional activity, e.g. DNA modification is important for controlling the accessibility for efficient switch recombination...
- IgE-mediated allergen gene vaccine platform targeting human antigen-presenting cells through the high-affinity IgE receptorAnne Behnecke
Hart and Louise Lyon Immunology Laboratory, Section of Clinical Immunology Allergy, Division of Pulmonary, Critical Care and Clinical Immunology Allergy, Department of Medicine, David Geffen School of Medicine at UCLA, Los Angeles, CA 90095, USA
J Allergy Clin Immunol 124:108-13. 2009..Allergen gene vaccination represents a promising alternative, but difficulties in gene targeting and expression in antigen-presenting cells represent a major limitation for efficient gene vaccination...
- B lymphocyte stimulator activates p38 mitogen-activated protein kinase in human Ig class switch recombinationTakechiyo Yamada
Division of Clinical Immunology, Department of Medicine, UCLA School of Medicine, 10833 Le Conte Ave, Los Angeles, CA 90095 1680, USA
Am J Respir Cell Mol Biol 32:388-94. 2005..These results indicate that BLyS-activated p38 MAPK plays an essential role in BLyS-induced AID-expression and CSR in human B cells...
- Human activation-induced cytidine deaminase is induced by IL-4 and negatively regulated by CD45: implication of CD45 as a Janus kinase phosphatase in antibody diversificationCheng Zhou
Hart and Louis Laboratory, Division of Clinical Immunology Allergy, Department of Medicine, University of California Los Angeles School of Medicine, 52 175 Center for Health Science, 10833 Le Conte Avenue, Los Angeles, CA 90095 1680, USA
J Immunol 170:1887-93. 2003..This study indicates that the JAK phosphatase activity of CD45 can be induced by anti-CD45 Ab treatment, and this principle may find clinical application in modulation of JAK activation in immune-mediated diseases...
- Impact of phosphorylation and phosphorylation-null mutants on the activity and deamination specificity of activation-induced cytidine deaminasePhuong Pham
Department of Biological Sciences, University of Southern California, Los Angeles, California 90089 2910, USA
J Biol Chem 283:17428-39. 2008..Both motifs occur with exceptionally high frequency in human switch regions, suggesting a possible relationship between AID deamination specificity and a loss of antibody diversification...
- Association of Ig/BCL6 translocations with germinal center B lymphocytes in human lymphoid tissues: implications for malignant transformationXuwei Yang
Hart and Louise Lyon Immunology Laboratory, Division of Clinical Immunology, Department of Medicine, David Geffen School of Medicine, University of California Los Angeles, 10833 Le Conte Ave, Los Angeles, CA 90095 1680, USA
Blood 108:2006-12. 2006..This study shows that Ig/BCL6 translocations occur in germinal center-stage B cells in healthy humans, and that Ig/BCL6 CTs per se are not likely sufficient to cause the malignant transformation in the context of human B cells...
- Co-aggregation of FcgammaRII with FcepsilonRI on human mast cells inhibits antigen-induced secretion and involves SHIP-Grb2-Dok complexesChristopher L Kepley
Department of Internal Medicine, Virginia Commonwealth University Health Systems, Richmond, Virginia 23298, USA
J Biol Chem 279:35139-49. 2004..Our results demonstrate how human FcepsilonRI-mediated responses can be inhibited by co-aggregation with FcgammaRIIB and implicate Dok, SHIP, and Grb2 as key intermediates in regulating antigen-induced mediator release...
- FcepsilonRI-FcgammaRII coaggregation inhibits IL-16 production from human Langerhans-like dendritic cellsChristopher L Kepley
Department of Internal Medicine, Virginia Commonwealth University, Medical College of Virginia, Richmond, VA, USA
Clin Immunol 108:89-94. 2003....
- A chimeric human-cat Fcgamma-Fel d1 fusion protein inhibits systemic, pulmonary, and cutaneous allergic reactivity to intratracheal challenge in mice sensitized to Fel d1, the major cat allergenTetsuya Terada
Department of Otorhinolaryngology, Osaka Medical College, Takatsuki, Osaka 569 8686, Japan
Clin Immunol 120:45-56. 2006....
- Rad6-Rad18 mediates a eukaryotic SOS response by ubiquitinating the 9-1-1 checkpoint clampYu Fu
Department of Microbiology and Immunology, University of Saskatchewan, Saskatoon, SK, S7N 5E5, Canada
Cell 133:601-11. 2008....
- Retrotransposon Tf1 is targeted to Pol II promoters by transcription activatorsYoung Eun Leem
Section on Eukaryotic Transposable Elements, Laboratory of Gene Regulation and Development, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA
Mol Cell 30:98-107. 2008..Surprisingly, we found Tf1 contained sequences that activated transcription, and these substituted for elements of the ade6 promoter disrupted by integration...
- Cell cycle control of centromeric repeat transcription and heterochromatin assemblyEe Sin Chen
Laboratory of Biochemistry and Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
Nature 451:734-7. 2008..Together, these processes probably facilitate heterochromatin maintenance through successive cell divisions...
- Tissue engineered cartilage from hTGF beta2 transduced human adipose derived stem cells seeded in PLGA/alginate compound in vitro and in vivoXiao bing Jin
Orthopaedics Department, Peking University Third Hospital, Beijing, People s Republic of China
J Biomed Mater Res A 86:1077-87. 2008..Ad5-hTGF beta2 transduced hASCs combined with three-dimensional PLGA/alginate compound may be a viable method in treating injuries of cartilage...
- PKHD1 gene silencing may cause cell abnormal proliferation through modulation of intracellular calcium in autosomal recessive polycystic kidney diseaseJiyun Yang
Department of Medical Genetics, West China Hospital, Sichuan University, Guoxue Xiang No 37, Chengdu 610041, PR China
J Biochem Mol Biol 40:467-74. 2007..Our results suggest that the loss of fibrocystin may lead to abnormal proliferation in kidney epithelial cells and cyst formation in ARPKD by modulation of intracellular Ca(2+)...
- Effects of dietary calorie restriction or exercise on the PI3K and Ras signaling pathways in the skin of miceLinglin Xie
Department of Human Nutrition and Statistics, Kansas State University, Manhattan, Kansas 66506, USA
J Biol Chem 282:28025-35. 2007..Selective inhibition of PI3K by PF+Exe but not AL+Exe seems more attributable to the magnitude of the caloric deficit and/or body fat loss than diet versus exercise comparison...
- Proteases from Schistosoma mansoni cercariae cleave IgE at solvent exposed interdomain regionsAkhmed Aslam
Institute of Genetics, University of Nottingham, Queen s Medical Centre, Nottingham, UK
Mol Immunol 45:567-74. 2008..Indeed, the finding may raise new possibilities for treatment of IgE-mediated allergic reactions mediated through Fcepsilon-receptors...
- PKD1 inhibits cancer cells migration and invasion via Wnt signaling pathway in vitroKe Zhang
State Key Laboratory of Biotherapy and Cancer Center, West China Medical School, and School of Life Science, Sichuan University, Chengdu 610041, China
Cell Biochem Funct 25:767-74. 2007..And we also showed that PC-1 regulated these processes in a process that may be at least partially through the Wnt pathway. Collectively, our data suggest that PKD1 may act as a novel member of the tumor suppressor family of genes...
- Ectopic neocartilage formation from predifferentiated human adipose derived stem cells induced by adenoviral-mediated transfer of hTGF beta2Xiao bing Jin
Orthopaedics Department, Peking University, Third Hospital, Beijing 100083, PR China
Biomaterials 28:2994-3003. 2007....
- Fully human monoclonal antibodies to hepatocyte growth factor with therapeutic potential against hepatocyte growth factor/c-Met-dependent human tumorsTeresa Burgess
Department of Oncology Research, Amgen, Inc, One Amgen Center Drive, Thousand Oaks, CA 91320 1799, USA
Cancer Res 66:1721-9. 2006..These results suggest that an antibody to an epitope in the beta-chain of HGF has potential as a novel therapeutic agent for treating patients with HGF-dependent tumors...
- Impact of Anti-IL-4 Therapy on Immune PolarizationKe Zhang; Fiscal Year: 2003..Overall, the experiments in this proposal will provide direct information as to the impacts of anti-IL-4 therapy on antibody responses and immune responses and by implication, the role of IL-4 in vivo in humans. ..