William Zagotta

Summary

Affiliation: University of Washington
Country: USA

Publications

  1. pmc Dynamics of Ca2+-calmodulin-dependent inhibition of rod cyclic nucleotide-gated channels measured by patch-clamp fluorometry
    Matthew C Trudeau
    Dept of Physiology and Biophysics, Box 357290, University of Washington, Seattle, WA 98195 7290, USA
    J Gen Physiol 124:211-23. 2004
  2. pmc Salt bridges and gating in the COOH-terminal region of HCN2 and CNGA1 channels
    Kimberley B Craven
    Department of Physiology and Biophysics, University of Washington, Seattle, WA 98195 7290, USA
    J Gen Physiol 124:663-77. 2004
  3. pmc A cysteine scan of the inner vestibule of cyclic nucleotide-gated channels reveals architecture and rearrangement of the pore
    Galen E Flynn
    Department of Physiology and Biophysics, University of Washington, Seattle 98195 7290, USA
    J Gen Physiol 121:563-82. 2003
  4. pmc Short-range molecular rearrangements in ion channels detected by tryptophan quenching of bimane fluorescence
    Leon D Islas
    Department of Physiology and Biophysics, Howard Hughes Medical Institute, University of Washington, Seattle, WA 98195, USA
    J Gen Physiol 128:337-46. 2006
  5. pmc Molecular mechanism underlying phosphatidylinositol 4,5-bisphosphate-induced inhibition of SpIH channels
    Galen E Flynn
    Department of Physiology and Biophysics, Howard Hughes Medical Institute, University of Washington, Seattle, Washington 98195 7290, USA
    J Biol Chem 286:15535-42. 2011
  6. pmc Flavonoid regulation of EAG1 channels
    Anne E Carlson
    Department of Physiology and Biophysics, University of Washington School of Medicine, Seattle, WA 98195, USA
    J Gen Physiol 141:347-58. 2013
  7. pmc Molecular mechanism for 3:1 subunit stoichiometry of rod cyclic nucleotide-gated ion channels
    Noah G Shuart
    Howard Hughes Medical Institute and Department of Physiology and Biophysics, University of Washington School of Medicine, Seattle, USA
    Nat Commun 2:457. 2011
  8. pmc Structure of the carboxy-terminal region of a KCNH channel
    Tinatin I Brelidze
    Department of Physiology and Biophysics, University of Washington School of Medicine, Box 357290, Seattle, Washington 98195 7290, USA
    Nature 481:530-3. 2012
  9. ncbi request reprint Structural basis for modulation and agonist specificity of HCN pacemaker channels
    William N Zagotta
    Department of Physiology and Biophysics, Howard Hughes Medical Institute, Box 357290, University of Washington School of Medicine, Seattle, Washington 98195 7290, USA
    Nature 425:200-5. 2003
  10. pmc Structure and rearrangements in the carboxy-terminal region of SpIH channels
    Galen E Flynn
    Department of Physiology and Biophysics, Howard Hughes Medical Institute, University of Washington, Seattle, WA 98195, USA
    Structure 15:671-82. 2007

Collaborators

Detail Information

Publications31

  1. pmc Dynamics of Ca2+-calmodulin-dependent inhibition of rod cyclic nucleotide-gated channels measured by patch-clamp fluorometry
    Matthew C Trudeau
    Dept of Physiology and Biophysics, Box 357290, University of Washington, Seattle, WA 98195 7290, USA
    J Gen Physiol 124:211-23. 2004
    ..This motion occurs with the same time course as channel inhibition, consistent with the notion that rearrangement of the NH(2)- and COOH-terminal regions underlies Ca(2+)/CaM-dependent inhibition...
  2. pmc Salt bridges and gating in the COOH-terminal region of HCN2 and CNGA1 channels
    Kimberley B Craven
    Department of Physiology and Biophysics, University of Washington, Seattle, WA 98195 7290, USA
    J Gen Physiol 124:663-77. 2004
    ..Discovering that one portion of the COOH terminus, the CNBD, can be in the activated configuration while the other portion, the C-linker, is not activated has lead us to suggest a novel modular gating scheme for HCN and CNG channels...
  3. pmc A cysteine scan of the inner vestibule of cyclic nucleotide-gated channels reveals architecture and rearrangement of the pore
    Galen E Flynn
    Department of Physiology and Biophysics, University of Washington, Seattle 98195 7290, USA
    J Gen Physiol 121:563-82. 2003
    ....
  4. pmc Short-range molecular rearrangements in ion channels detected by tryptophan quenching of bimane fluorescence
    Leon D Islas
    Department of Physiology and Biophysics, Howard Hughes Medical Institute, University of Washington, Seattle, WA 98195, USA
    J Gen Physiol 128:337-46. 2006
    ..The approach offers new hope for elucidating the gating rearrangements in channels of known structure...
  5. pmc Molecular mechanism underlying phosphatidylinositol 4,5-bisphosphate-induced inhibition of SpIH channels
    Galen E Flynn
    Department of Physiology and Biophysics, Howard Hughes Medical Institute, University of Washington, Seattle, Washington 98195 7290, USA
    J Biol Chem 286:15535-42. 2011
    ....
  6. pmc Flavonoid regulation of EAG1 channels
    Anne E Carlson
    Department of Physiology and Biophysics, University of Washington School of Medicine, Seattle, WA 98195, USA
    J Gen Physiol 141:347-58. 2013
    ..EAG1 channels should be considered as a possible target for the physiological effects of flavonoids...
  7. pmc Molecular mechanism for 3:1 subunit stoichiometry of rod cyclic nucleotide-gated ion channels
    Noah G Shuart
    Howard Hughes Medical Institute and Department of Physiology and Biophysics, University of Washington School of Medicine, Seattle, USA
    Nat Commun 2:457. 2011
    ..The X-ray crystal structures of the parallel 3-helix coiled-coil domains of CNGA1 and CNGA3 subunits were similar, suggesting that a similar mechanism controls the stoichiometry of cone CNG channels...
  8. pmc Structure of the carboxy-terminal region of a KCNH channel
    Tinatin I Brelidze
    Department of Physiology and Biophysics, University of Washington School of Medicine, Box 357290, Seattle, Washington 98195 7290, USA
    Nature 481:530-3. 2012
    ..These results provide a structural framework for understanding the regulation of ion channels in the KCNH family by the C-linker/CNBHD and may guide the design of specific drugs...
  9. ncbi request reprint Structural basis for modulation and agonist specificity of HCN pacemaker channels
    William N Zagotta
    Department of Physiology and Biophysics, Howard Hughes Medical Institute, Box 357290, University of Washington School of Medicine, Seattle, Washington 98195 7290, USA
    Nature 425:200-5. 2003
    ..On the basis of amino acid sequence similarity to HCN channels, the cyclic nucleotide-gated, and eag- and KAT1-related families of channels are probably related to HCN channels in structure and mechanism...
  10. pmc Structure and rearrangements in the carboxy-terminal region of SpIH channels
    Galen E Flynn
    Department of Physiology and Biophysics, Howard Hughes Medical Institute, University of Washington, Seattle, WA 98195, USA
    Structure 15:671-82. 2007
    ..These mechanisms will likely extend to other cyclic nucleotide-regulated channels and enzymes as well...
  11. ncbi request reprint Multimerization of the ligand binding domains of cyclic nucleotide-gated channels
    Kimberly Matulef
    Molecular and Cellular Biology Program, University of Washington, Seattle, WA 98195, USA
    Neuron 36:93-103. 2002
    ..These data suggest that the C helices from all four channel subunits are in close proximity in the closed state and move apart during channel opening...
  12. ncbi request reprint Rod cyclic nucleotide-gated channels have a stoichiometry of three CNGA1 subunits and one CNGB1 subunit
    Jie Zheng
    Howard Hughes Medical Institute, Seattle, WA 98195, USA
    Neuron 36:891-6. 2002
    ..We find, surprisingly, that the channel contains three CNGA1 subunits and only one CNGB1 subunit. These results have implications for CNG channel function in particular and assembly of membrane proteins in general...
  13. pmc C-terminal movement during gating in cyclic nucleotide-modulated channels
    Kimberley B Craven
    Department of Physiology and Biophysics, University of Washington, Seattle, WA 98195, USA
    J Biol Chem 283:14728-38. 2008
    ..These results support our hypothesis that the SB triad forms in the closed channel and indicate that this region expands as the channel opens...
  14. ncbi request reprint CNG and HCN channels: two peas, one pod
    Kimberley B Craven
    Department of Physiology and Biophysics, University of Washington, Seattle, WA 98195, USA
    Annu Rev Physiol 68:375-401. 2006
    ..We focus on how similarities in structure and activation mechanisms result in common biophysical models, allowing CNG and HCN channels to be viewed as a single genre...
  15. ncbi request reprint Disruption of an intersubunit interaction underlies Ca2+-calmodulin modulation of cyclic nucleotide-gated channels
    Jie Zheng
    Howard Hughes Medical Institute and Department of Physiology and Biophysics, University of Washington School of Medicine, Seattle, Washington 98195 7290, USA
    J Neurosci 23:8167-75. 2003
    ..The quaternary arrangement presents a structural framework for understanding the molecular mechanism of olfactory adaptation...
  16. pmc Mapping the structure and conformational movements of proteins with transition metal ion FRET
    Justin W Taraska
    Department of Physiology and Biophysics, Howard Hughes Medical Institute, University of Washington, Seattle, Washington, USA
    Nat Methods 6:532-7. 2009
    ..Our results suggest a general model for the conformational switch in the cyclic nucleotide-binding site of cyclic nucleotide-regulated ion channels...
  17. ncbi request reprint Calcium/calmodulin modulation of olfactory and rod cyclic nucleotide-gated ion channels
    Matthew C Trudeau
    Department of Physiology and Biophysics, Howard Hughes Medical Institute, University of Washington Medical School, Seattle, Washington 98195, USA
    J Biol Chem 278:18705-8. 2003
    ..Here, we will review recent advances in understanding the subunit composition and the mechanisms and roles for Ca2+/CaM-dependent inhibition in olfactory and rod CNG channels...
  18. pmc Mechanism of calcium/calmodulin inhibition of rod cyclic nucleotide-gated channels
    Matthew C Trudeau
    Department of Physiology and Biophysics, Howard Hughes Medical Institute, University of Washington Medical School, Box 357370, Seattle, WA 98195, USA
    Proc Natl Acad Sci U S A 99:8424-9. 2002
    ..The mechanism underlying Ca(2+)/CaM modulation of rod channels is distinct from that in olfactory (CNGA2) CNG channels...
  19. pmc Absence of direct cyclic nucleotide modulation of mEAG1 and hERG1 channels revealed with fluorescence and electrophysiological methods
    Tinatin I Brelidze
    Department of Physiology and Biophysics, University of Washington School of Medicine, Seattle, Washington 98195, USA
    J Biol Chem 284:27989-97. 2009
    ..Further studies are necessary to determine if the CNBD in the EAG family of K(+) channels might harbor a binding site for a ligand yet to be uncovered...
  20. ncbi request reprint Patch-clamp fluorometry recording of conformational rearrangements of ion channels
    Jie Zheng
    Howard Hughes Medical Institute, Department of Physiology and Biophysics, University of Washington School of Medicine, Box 357290, Seattle, WA 98195, USA
    Sci STKE 2003:PL7. 2003
    ..Combined with fluorescence resonance energy transfer (FRET), the technique should shed new light on ion channel activation, regulation, and interaction with other membrane proteins...
  21. pmc The carboxyl-terminal region of cyclic nucleotide-modulated channels is a gating ring, not a permeation path
    J P Johnson
    Howard Hughes Medical Institute and Department of Physiology and Biophysics, University of Washington School of Medicine, Box 357290, Seattle, WA 98195, USA
    Proc Natl Acad Sci U S A 102:2742-7. 2005
    ....
  22. pmc Ca2+-dependent desensitization of TRPV2 channels is mediated by hydrolysis of phosphatidylinositol 4,5-bisphosphate
    Jose Mercado
    Department of Physiology and Biophysics, University of Washington, Seattle, Washington 98195, USA
    J Neurosci 30:13338-47. 2010
    ..We conclude that the decrease in PIP(2) levels on channel activation underlies a major component of Ca(2+)-dependent desensitization of TRPV2 and may play a similar role in other TRP channels...
  23. ncbi request reprint Stoichiometry and assembly of olfactory cyclic nucleotide-gated channels
    Jie Zheng
    Howard Hughes Medical Institute, Department of Physiology and Biophysics, Box 357290, University of Washington School of Medicine, Seattle, WA 98195 USA
    Neuron 42:411-21. 2004
    ....
  24. pmc Short-distance probes for protein backbone structure based on energy transfer between bimane and transition metal ions
    Justin W Taraska
    Department of Physiology and Biophysics, Howard Hughes Medical Institute, University of Washington, Seattle, WA 98195, USA
    Proc Natl Acad Sci U S A 106:16227-32. 2009
    ..This improved method overcomes several of the key limitations of classical FRET for intramolecular distance measurements...
  25. ncbi request reprint An intersubunit interaction regulates trafficking of rod cyclic nucleotide-gated channels and is disrupted in an inherited form of blindness
    Matthew C Trudeau
    Department of Physiology and Biophysics, Howard Hughes Medical Institute, University of Washington Medical School, Seattle, WA 98195, USA
    Neuron 34:197-207. 2002
    ..Thus, disruption of a regulatory interaction by mutation in CNGA1 exposed a region of CNGB1 that disrupted surface expression of heteromeric CNGA1-RP/CNGB1 channels, accounting for this instance of RP...
  26. ncbi request reprint Structural dynamics in the gating ring of cyclic nucleotide-gated ion channels
    Justin W Taraska
    Department of Physiology and Biophysics, Howard Hughes Medical Institute, University of Washington, Seattle, Washington 98195, USA
    Nat Struct Mol Biol 14:854-60. 2007
    ..We conclude that during channel activation, a portion of the gating ring moves parallel to the plasma membrane, hinging toward the central axis of the channel...
  27. ncbi request reprint Cyclic nucleotide-regulated ion channels: spotlight on symmetry
    Justin W Taraska
    Structure 15:1023-4. 2007
    ..In this issue of Structure, Chiu et al. (2007) report the 16 A EM structure of the prokaryotic cyclic nucleotide-regulated K(+) channel MloK1. This structure reveals that the channel is arranged as a four-fold symmetric tetramer...
  28. ncbi request reprint Cyclic nucleotide-gated ion channels
    Kimberly Matulef
    Department of Molecular and Cellular Physiology, Stanford University, Stanford, California 94305, USA
    Annu Rev Cell Dev Biol 19:23-44. 2003
    ..From these studies, a picture is beginning to emerge for how the binding of cyclic nucleotide is transduced into the opening of the pore and how this allosteric transition is modulated by various physiological effectors...
  29. ncbi request reprint Membrane biology: permutations of permeability
    William N Zagotta
    Nature 440:427-9. 2006