Affiliation: University of Wisconsin
- Induced pluripotent stem cell lines derived from human somatic cellsJunying Yu
Genome Center of Wisconsin, Madison, WI 53706 1580, USA
Science 318:1917-20. 2007....
- Human induced pluripotent stem cells free of vector and transgene sequencesJunying Yu
Morgridge Institute for Research, Madison, WI 53707 7365, USA
Science 324:797-801. 2009..These results demonstrate that reprogramming human somatic cells does not require genomic integration or the continued presence of exogenous reprogramming factors and removes one obstacle to the clinical application of human iPS cells...
- Efficient generation of transgene-free induced pluripotent stem cells from normal and neoplastic bone marrow and cord blood mononuclear cellsKejin Hu
Wisconsin National Primate Research Center, University of Wisconsin, 1220 Capitol Court, Madison, WI 53715, USA
Blood 117:e109-19. 2011..This approach provides an opportunity to explore banked normal and diseased CB and BM samples without the limitations associated with virus-based methods...
- A mesoderm-derived precursor for mesenchymal stem and endothelial cellsMaxim A Vodyanik
National Primate Research Center, University of Wisconsin Graduate School, Madison, 53715, USA
Cell Stem Cell 7:718-29. 2010..Together, these studies identified a common precursor of mesenchymal and endothelial cells, mesenchymoangioblast, as the source of mesoderm-derived MSCs...
- Generation of red blood cells from human induced pluripotent stem cellsJessica Dias
Department of Pathology and Laboratory Medicine, University of Wisconsin, School of Medicine and Public Health, Madison, Wisconsin 53715, USA
Stem Cells Dev 20:1639-47. 2011..Since RBCs generated from transgene-free hiPSCs lack genomic integration and background expression of reprogramming genes, they would be a preferable cell source for modeling of diseases and for gene function studies...
- Extracellular matrix promotes highly efficient cardiac differentiation of human pluripotent stem cells: the matrix sandwich methodJianhua Zhang
Department of Medicine, School of Medicine and Public Health, University of Wisconsin, Madison, WI, USA
Circ Res 111:1125-36. 2012..However, extracellular matrix is also fundamentally involved in cardiac development from the earliest morphogenetic events, such as gastrulation...
- Functional cardiomyocytes derived from human induced pluripotent stem cellsJianhua Zhang
Department of Medicine, University of Wisconsin, WiCell Research Institute, Madison, WI 53792 3248, USA
Circ Res 104:e30-41. 2009..We conclude that human iPS cells can differentiate into functional cardiomyocytes, and thus iPS cells are a viable option as an autologous cell source for cardiac repair and a powerful tool for cardiovascular research...
- Protein kinase C mediated extraembryonic endoderm differentiation of human embryonic stem cellsXuezhu Feng
Genome Center of Wisconsin, University of Wisconsin Madison, Madison, WI, USA
Stem Cells 30:461-70. 2012..We further confirmed that this differentiation is through the activation of protein kinase C (PKC) pathway and demonstrated that a particular PKC subtype, PKC-δ, is most responsible for this transition...
- Neural differentiation of human induced pluripotent stem cells follows developmental principles but with variable potencyBao Yang Hu
Department of Anatomy, School of Medicine and Public Health, University of Wisconsin, Waisman Center, Madison, WI 53705, USA
Proc Natl Acad Sci U S A 107:4335-40. 2010..These findings, which show a need for improving differentiation potency of iPSCs, suggest the possibility of employing human iPSCs in pathological studies, therapeutic screening, and autologous cell transplantation...
- FGF2 sustains NANOG and switches the outcome of BMP4-induced human embryonic stem cell differentiationPengzhi Yu
Morgridge Institute for Research, Madison, WI 53715 7365, USA
Cell Stem Cell 8:326-34. 2011..Together, our results demonstrate that FGF2 signaling switches the outcome of BMP4-induced differentiation of human ESCs by maintaining NANOG levels through the MEK-ERK pathway...
- Induced pluripotent stem cells from a spinal muscular atrophy patientAllison D Ebert
The Waisman Center, The Stem Cell and Regenerative Medicine Center, University of Wisconsin Madison, 1500 Highland Avenue, Madison, Wisconsin 53705, USA
Nature 457:277-80. 2009..As such, it represents a promising resource to study disease mechanisms, screen new drug compounds and develop new therapies...
- Pluripotent stem cell linesJunying Yu
Genome Center of Wisconsin, University of Wisconsin Madison, Madison, Wisconsin 53706, USA
Genes Dev 22:1987-97. 2008..Here we review the family of pluripotent cell lines derived from early embryos and from germ cells, and compare them with the more recently described induced pluripotent stem cells...
- Human lymphoblastoid B-cell lines reprogrammed to EBV-free induced pluripotent stem cellsDeepika Rajesh
Cellular Dynamics International Inc, Madison, WI 53711 1018, USA
Blood 118:1797-800. 2011..Thus, reprogramming LCLs could offer an unlimited source for patient-specific iPSCs...
- Feeder-independent culture of human embryonic stem cellsTenneille E Ludwig
Genome Center of Wisconsin, University of Wisconsin Madison, 425 Henry Mall, Madison, Wisconsin 53706, USA
Nat Methods 3:637-46. 2006
- Pompe disease results in a Golgi-based glycosylation deficit in human induced pluripotent stem cell-derived cardiomyocytesKunil K Raval
From the Department of Medicine, School of Medicine and Public Health, University of Wisconsin, Madison, Wisconsin 53705, The WiCell Institute, Madison, Wisconsin 53719
J Biol Chem 290:3121-36. 2015..These data suggest that Pompe cardiomyopathy has a glycan processing abnormality and thus shares features with hypertrophic cardiomyopathies observed in the congenital disorders of glycosylation. ..
- Hematopoietic and endothelial differentiation of human induced pluripotent stem cellsKyung Dal Choi
Department of Pathology and Laboratory Medicine, University of Wisconsin, Madison, 53715, USA
Stem Cells 27:559-67. 2009....
- Human embryonic stem cells reprogram myeloid precursors following cell-cell fusionJunying Yu
Wisconsin National Primate Research Center, University of Wisconsin, Madison, WI 53706, USA
Stem Cells 24:168-76. 2006..These results indicate that hESCs are capable of reprogramming nuclei from differentiated cells and that hESC hybrid cells provide a new model system for studying the mechanisms of nuclear reprogramming...
- Deletion of the DNA/RNA-binding protein MSY2 leads to post-meiotic arrestJuxiang Yang
Center for Research on Reproduction and Women s Health, University of Pennsylvania School of Medicine, 1310 Biomedical Research Building II III, 421 Curie Boulevard, Philadelphia, PA 19104 6160, USA
Mol Cell Endocrinol 250:20-4. 2006..The germ cell specificity and the critical functions played by this multifunctional DNA- and RNA-binding protein during spermatogenesis make Contrin, the human ortholog of MSY2, an attractive and novel target for male contraception...
- Absence of the DNA-/RNA-binding protein MSY2 results in male and female infertilityJuxiang Yang
Center for Research on Reproduction and Women s Health and Department of Biology, University of Pennsylvania School of Medicine, 421 Curie Boulevard, Philadelphia, PA 19104, USA
Proc Natl Acad Sci U S A 102:5755-60. 2005..Thus, Msy2 represents one of a small number of germ-cell-specific genes whose deletion leads to the disruption of both spermatogenesis and oogenesis...
- Transgenic RNAi-mediated reduction of MSY2 in mouse oocytes results in reduced fertilityJunying Yu
Department of Biology, University of Pennsylvania, Philadelphia, PA 19104 6018, USA
Dev Biol 268:195-206. 2004..These results support a role for MSY2 in stabilizing maternal mRNAs in growing oocytes, a process essential to generate meiotically and developmentally competent oocytes...
- Requirement for RNA-binding activity of MSY2 for cytoplasmic localization and retention in mouse oocytesJunying Yu
Department of Biology, University of Pennsylvania, Philadelphia, PA 19104 6018, USA
Dev Biol 255:249-62. 2003..These results suggest that binding of intact maternal mRNA by MSY2 is required for its cytoplasmic retention...
- RNA-binding properties and translation repression in vitro by germ cell-specific MSY2 proteinJunying Yu
Department of Biology and Center for Research on Reproduction Women s Health, University of Pennsylvania, Philadelphia 19104 6018, USA
Biol Reprod 67:1093-8. 2002....