DAVID T contact WOODLEY

Summary

Affiliation: University of Southern California
Country: USA

Publications

  1. ncbi request reprint The role of IgE anti-basement membrane zone autoantibodies in bullous pemphigoid
    David T Woodley
    Department of Dermatology, Keck School of Medicine, University of Southern California, Los Angeles, CA 90033, USA
    Arch Dermatol 143:249-50. 2007
  2. ncbi request reprint The epidermolysis bullosa acquisita antigen (type VII collagen) is present in human colon and patients with crohn's disease have autoantibodies to type VII collagen
    Mei Chen
    Department of Medicine, Division of Dermatology, University of Southern California, Los Angeles, California 90033, USA
    J Invest Dermatol 118:1059-64. 2002
  3. ncbi request reprint PKCdelta clustering at the leading edge and mediating growth factor-enhanced, but not ecm-initiated, dermal fibroblast migration
    Jianhua Fan
    The Department of Dermatology and the Norris Comprehensive Cancer Center, The University of Southern California Keck School of Medicine, Los Angeles, California, USA
    J Invest Dermatol 126:1233-43. 2006
  4. ncbi request reprint A patient with both bullous pemphigoid and epidermolysis bullosa acquisita: an example of intermolecular epitope spreading
    Janet A Fairley
    Department of Dermatology, Medical College of Wisconsin, Milwaukee 53226, USA
    J Am Acad Dermatol 51:118-22. 2004
  5. ncbi request reprint Restoration of type VII collagen expression and function in dystrophic epidermolysis bullosa
    Mei Chen
    Department of Medicine, Division of Dermatology, University of Southern California, CRL 204, 1303 Mission Road, Los Angeles, California 90033, USA
    Nat Genet 32:670-5. 2002
  6. ncbi request reprint Intravenously injected human fibroblasts home to skin wounds, deliver type VII collagen, and promote wound healing
    David T Woodley
    Department of Dermatology, The Keck School of Medicine, University of Southern California, Los Angeles, California, USA
    Mol Ther 15:628-35. 2007
  7. pmc A "traffic control" role for TGFbeta3: orchestrating dermal and epidermal cell motility during wound healing
    Balaji Bandyopadhyay
    Department of Dermatology and Norris Comprehensive Cancer Center, University of Southern California Keck School of Medicine, Los Angeles, CA 90033, USA
    J Cell Biol 172:1093-105. 2006
  8. ncbi request reprint Intradermal injection of lentiviral vectors corrects regenerated human dystrophic epidermolysis bullosa skin tissue in vivo
    David T Woodley
    Division of Dermatology, Department of Medicine, University of Southern California, Los Angeles, CA, 90033, USA
    Mol Ther 10:318-26. 2004
  9. pmc Injection of recombinant human type VII collagen corrects the disease phenotype in a murine model of dystrophic epidermolysis bullosa
    Jennifer Remington
    Department of Dermatology, University of Southern California, Los Angeles, California, USA
    Mol Ther 17:26-33. 2009
  10. doi request reprint Non-compensating roles between Nckalpha and Nckbeta in PDGF-BB signaling to promote human dermal fibroblast migration
    Shengxi Guan
    Department of Dermatology and USC Norris Comprehensive Cancer Center, Keck School of Medicine, The University of Southern California, Los Angeles, California 90033, USA
    J Invest Dermatol 129:1909-20. 2009

Research Grants

  1. Mechanism of Keratinocyte Motility
    David Woodley; Fiscal Year: 2009

Collaborators

Detail Information

Publications37

  1. ncbi request reprint The role of IgE anti-basement membrane zone autoantibodies in bullous pemphigoid
    David T Woodley
    Department of Dermatology, Keck School of Medicine, University of Southern California, Los Angeles, CA 90033, USA
    Arch Dermatol 143:249-50. 2007
  2. ncbi request reprint The epidermolysis bullosa acquisita antigen (type VII collagen) is present in human colon and patients with crohn's disease have autoantibodies to type VII collagen
    Mei Chen
    Department of Medicine, Division of Dermatology, University of Southern California, Los Angeles, California 90033, USA
    J Invest Dermatol 118:1059-64. 2002
    ..It was concluded that patients with inflammatory bowel disease may have IgG autoantibodies to type VII collagen, which exists in both the skin and the gut...
  3. ncbi request reprint PKCdelta clustering at the leading edge and mediating growth factor-enhanced, but not ecm-initiated, dermal fibroblast migration
    Jianhua Fan
    The Department of Dermatology and the Norris Comprehensive Cancer Center, The University of Southern California Keck School of Medicine, Los Angeles, California, USA
    J Invest Dermatol 126:1233-43. 2006
    ..Instead, inhibition of PKCdelta blocks PDGF-BB-stimulated activation of signal transducer and activator of transcription 3 (Stat3). This study unveiled the specificity of PKCdelta in the control of DF migration...
  4. ncbi request reprint A patient with both bullous pemphigoid and epidermolysis bullosa acquisita: an example of intermolecular epitope spreading
    Janet A Fairley
    Department of Dermatology, Medical College of Wisconsin, Milwaukee 53226, USA
    J Am Acad Dermatol 51:118-22. 2004
    ..The analysis of the patient's autoantibodies provides strong evidence for the involvement of epitope spreading in the evolution of his autoimmune disease...
  5. ncbi request reprint Restoration of type VII collagen expression and function in dystrophic epidermolysis bullosa
    Mei Chen
    Department of Medicine, Division of Dermatology, University of Southern California, CRL 204, 1303 Mission Road, Los Angeles, California 90033, USA
    Nat Genet 32:670-5. 2002
    ..These studies demonstrate that it is possible to restore type VII collagen gene expression in RDEB skin in vivo...
  6. ncbi request reprint Intravenously injected human fibroblasts home to skin wounds, deliver type VII collagen, and promote wound healing
    David T Woodley
    Department of Dermatology, The Keck School of Medicine, University of Southern California, Los Angeles, California, USA
    Mol Ther 15:628-35. 2007
    ..This is also the first evidence that IV injected, molecularly engineered skin fibroblasts can deliver C7 to skin wounds. This strategy could be useful for treating DEB patients...
  7. pmc A "traffic control" role for TGFbeta3: orchestrating dermal and epidermal cell motility during wound healing
    Balaji Bandyopadhyay
    Department of Dermatology and Norris Comprehensive Cancer Center, University of Southern California Keck School of Medicine, Los Angeles, CA 90033, USA
    J Cell Biol 172:1093-105. 2006
    ..Therefore, the naturally occurring plasma-->serum-->plasma transition during wound healing orchestrates the orderly migration of dermal and epidermal cells...
  8. ncbi request reprint Intradermal injection of lentiviral vectors corrects regenerated human dystrophic epidermolysis bullosa skin tissue in vivo
    David T Woodley
    Division of Dermatology, Department of Medicine, University of Southern California, Los Angeles, CA, 90033, USA
    Mol Ther 10:318-26. 2004
    ..These data demonstrate efficient and long-term type VII collagen gene transfer in vivo using direct intradermal injection of an engineered lentiviral vector...
  9. pmc Injection of recombinant human type VII collagen corrects the disease phenotype in a murine model of dystrophic epidermolysis bullosa
    Jennifer Remington
    Department of Dermatology, University of Southern California, Los Angeles, California, USA
    Mol Ther 17:26-33. 2009
    ..Anti-C7 antibody production was prevented by treating the mice with an anti-CD40L monoclonal antibody, MR1. We conclude that protein therapy may be feasible for the treatment of human patients with RDEB...
  10. doi request reprint Non-compensating roles between Nckalpha and Nckbeta in PDGF-BB signaling to promote human dermal fibroblast migration
    Shengxi Guan
    Department of Dermatology and USC Norris Comprehensive Cancer Center, Keck School of Medicine, The University of Southern California, Los Angeles, California 90033, USA
    J Invest Dermatol 129:1909-20. 2009
    ..Thus, this study has elucidated the independent roles and mechanisms of action of Nckalpha and Nckbeta in DF migration, which is critical for wound healing...
  11. ncbi request reprint Familial epidermolysis bullosa acquisita
    Megan H Noe
    Department of Dermatology, University of Iowa, Iowa City, Iowa, USA
    Dermatol Online J 14:2. 2008
    ..These findings provide support for the hypothesis that there is a genetic component to EBA...
  12. pmc Extracellular heat shock protein-90alpha: linking hypoxia to skin cell motility and wound healing
    Wei Li
    Department of Dermatology, University of Southern California Keck School of Medicine, Los Angeles, CA 90033, USA
    EMBO J 26:1221-33. 2007
    ..This study has demonstrated a novel mechanism of hypoxia>HIF-1>hsp90alpha secretion>skin cell migration>wound healing, and identified extracellular hsp90alpha as a potential therapeutic agent for skin wounds...
  13. ncbi request reprint The recombinant expression of full-length type VII collagen and characterization of molecular mechanisms underlying dystrophic epidermolysis bullosa
    Mei Chen
    Department of Medicine, Division of Dermatology, Division of Plastic and Reconstructive Surgery, University of Southern California, Los Angeles, California 90033, USA
    J Biol Chem 277:2118-24. 2002
    ..We conclude that structural and functional studies of in vitro generated type VII collagen mutant proteins will aid in correlating genetic mutations with the clinical phenotypes of DEB patients...
  14. pmc The cartilage matrix protein subdomain of type VII collagen is pathogenic for epidermolysis bullosa acquisita
    Mei Chen
    Department of Dermatology, The Keck School of Medicine, University of Southern California, 1303 Mission Road, Los Angeles, CA 90033, USA
    Am J Pathol 170:2009-18. 2007
    ..This is the first antigenic epitope on type VII collagen demonstrated to be a pathogenic target for EBA autoantibodies...
  15. ncbi request reprint Autoimmunity to type VII collagen: epidermolysis bullosa acquisita
    David T Woodley
    Department of Dermatology, The Keck School of Medicine, University of Southern California, Los Angeles, CA, USA
    Clin Rev Allergy Immunol 33:78-84. 2007
    ..Treatment for EBA is unsatisfactory. Some therapeutic success has been reported with colchichine, dapsone, photopheresis, infliximab, and IVIG...
  16. pmc Characterization of molecular mechanisms underlying mutations in dystrophic epidermolysis bullosa using site-directed mutagenesis
    David T Woodley
    Department of Dermatology, The Keck School of Medicine at the University of Southern California, Los Angeles, CA 90033, USA
    J Biol Chem 283:17838-45. 2008
    ..We conclude that known recessive DEB C7 mutations perturb critical functions of the C7 molecule and likely contribute to the clinical phenotypes of DEB patients...
  17. doi request reprint Autoimmunity to type VII collagen: epidermolysis bullosa acquisita
    Jennifer Remington
    Department of Dermatology, Keck School of Medicine, University of Southern California, Los Angeles, CA 90033, USA
    Curr Dir Autoimmun 10:195-205. 2008
    ..Treatment for EBA is unsatisfactory, however, some therapeutic success has been reported with colchicine, dapsone, photophoresis, infliximab and intravenous immunoglobulin...
  18. ncbi request reprint Induction of epidermolysis bullosa acquisita in mice by passive transfer of autoantibodies from patients
    David T Woodley
    Department of Dermatology, The Keck School of Medicine at the University of Southern California, Los Angeles, California, USA
    J Invest Dermatol 126:1323-30. 2006
    ..These passive transfer studies demonstrate that human EBA autoantibodies are pathogenic. This novel EBA mouse model can be used to further investigate EBA autoimmunity and to develop possible therapies...
  19. ncbi request reprint Normal and gene-corrected dystrophic epidermolysis bullosa fibroblasts alone can produce type VII collagen at the basement membrane zone
    David T Woodley
    Department of Medicine, Division of Dermatology, University of Southern California, Los Angeles, California 90033, USA
    J Invest Dermatol 121:1021-8. 2003
    ....
  20. ncbi request reprint Pterygium inversum unguis associated with stroke
    Manjunath Vadmal
    Department of Dermatology, University of Southern California, Keck School of Medicine and Veterans Administration, Los Angeles, California, USA
    J Am Acad Dermatol 53:501-3. 2005
    ..We describe an unusual case of acquired PIU of the right fingernails alone associated with a cerebral vascular accident resulting in right-sided paralysis and sensory deficit. We also present the first histologic features of PIU...
  21. ncbi request reprint Evidence that anti-type VII collagen antibodies are pathogenic and responsible for the clinical, histological, and immunological features of epidermolysis bullosa acquisita
    David T Woodley
    The Division of Dermatology, The Keck School of Medicine at the University of Southern California, Los Angeles, California 90033, USA
    J Invest Dermatol 124:958-64. 2005
    ....
  22. ncbi request reprint An important role for protein kinase C-delta in human keratinocyte migration on dermal collagen
    Wei Li
    Department of Medicine, Norris Cancer Center, Los Angeles, California 90033, USA
    Exp Cell Res 273:219-28. 2002
    ..Furthermore, PKCdelta is activated in migrating human keratinocytes. These observations indicate for the first time that the PKCdelta pathway plays an important role in the control of human keratinocyte migration...
  23. ncbi request reprint Migration of human keratinocytes in plasma and serum and wound re-epithelialisation
    Ginard Henry
    Division of Plastic Surgery, Department of Surgery, Keck School of Medicine, University of Southern California, Los Angeles, CA 90033, USA
    Lancet 361:574-6. 2003
    ..We also showed that induced keratinocyte polarisation, activation of p38MAPK, and production of matrix metalloprotease 9 are possible mechanisms for promotion of re-epithelialisation of skin wounds by human serum...
  24. ncbi request reprint Signals that initiate, augment, and provide directionality for human keratinocyte motility
    Wei Li
    The Department of Medicine, Division of Dermatology and the Norris Cancer Center, University of Southern California Keck School of Medicine, Los Angeles, California, USA
    J Invest Dermatol 123:622-33. 2004
    ..Co-expression of both constitutively activated p38 and ERK1/2, however, could partially mimic the pro-motility effects of collagen and GF. This study reveals for the first time the specific functions of ECM and GF in cell motility...
  25. ncbi request reprint Epidermolysis bullosa: then and now
    David T Woodley
    Division of Dermatology, The Keck School of Medicine, University of Southern California, Los Angeles, CA 90033, USA
    J Am Acad Dermatol 51:S55-7. 2004
  26. pmc Mechanism of human dermal fibroblast migration driven by type I collagen and platelet-derived growth factor-BB
    Wei Li
    The Division of Dermatology and the University of Southern California Norris Cancer Center, University of Southern California Keck School of Medicine, Los Angeles, California 90033, USA
    Mol Biol Cell 15:294-309. 2004
    ..The experiments demonstrate 1) the genes' individual roles and specificities, 2) their combined effects and sufficiency, and 3) the mechanisms of their intermolecular connections in HDF migration driven by collagen and PDGF-BB...
  27. pmc Transforming growth factor alpha (TGFalpha)-stimulated secretion of HSP90alpha: using the receptor LRP-1/CD91 to promote human skin cell migration against a TGFbeta-rich environment during wound healing
    Chieh Fang Cheng
    Department of Dermatology, University of Southern California Keck School of Medicine, 1441 Eastlake Ave, Room 6320, Los Angeles, CA 90033, USA
    Mol Cell Biol 28:3344-58. 2008
    ..This finding provides a long-sought answer to the question of how dermal cells migrate into the wound environment to build new connective tissues and blood vessels. Thus, secreted hsp90alpha is potentially a new agent for wound healing...
  28. pmc Participation of the lipoprotein receptor LRP1 in hypoxia-HSP90alpha autocrine signaling to promote keratinocyte migration
    David T Woodley
    Department of Dermatology and the USC Norris Comprehensive Cancer Center, The University of Southern California Keck School of Medicine, Los Angeles, CA 90033, USA
    J Cell Sci 122:1495-8. 2009
    ..We propose that the hypoxia-HSP90alpha-LRP1 autocrine loop provides previously unrecognized therapeutic targets for human disorders such as chronic wounds and cancer invasion...
  29. doi request reprint Profiling motility signal-specific genes in primary human keratinocytes
    Chieh Fang Cheng
    Department of Dermatology and the USC Norris Cancer Center, The University of Southern California Keck School of Medicine, Los Angeles, California 90089, USA
    J Invest Dermatol 128:1981-90. 2008
    ..Three genes, all encoding secreted molecules, were investigated in HK migration. These cell motility-specific gene profiles may prove useful to skin wound healing...
  30. ncbi request reprint Injection of recombinant human type VII collagen restores collagen function in dystrophic epidermolysis bullosa
    David T Woodley
    Department of Medicine, Division of Dermatology, University of Southern California, CRL 204, 1303 Mission Road, Los Angeles, California 90033, USA
    Nat Med 10:693-5. 2004
    ..The injected collagen localized to the basement membrane zone of both types of tissues, was organized into human anchoring fibril structures and reversed the features of DEB disease in the DEB skin equivalent...
  31. ncbi request reprint Wound-healing perspectives
    Wei Li
    University of Southern California Dermatology, Norris Cancer Center, Keck School of Medicine, Los Angeles, 90033, USA
    Dermatol Clin 23:181-92. 2005
    ..The article then discusses selected clinical advances that are based less on evidence-based observation and more on what works in practice and promotes wound healing...
  32. ncbi request reprint Transforming growth factor-alpha: a major human serum factor that promotes human keratinocyte migration
    Yong Li
    Department of Dermatology and the USC Norris Comprehensive Cancer Center, USC Laboratory for Investigative Dermatology, University of Southern California Keck School of Medicine, Los Angeles, USA
    J Invest Dermatol 126:2096-105. 2006
    ..Since insulin levels are identical between plasma and serum, we propose that TGFalpha is the physiologic HK pro-motility factor in HS...
  33. ncbi request reprint Fibroblasts as target cells for DEB gene therapy
    Mei Chen
    Department of Dermatology, Keck School of Medicine, University of Southern California, Los Angeles, California 90033, USA
    J Invest Dermatol 126:708-10. 2006
    ..With regard to molecular engineering, the dermal fibroblast has advantages over epidermal keratinocytes for delivering C7 to DEB patients...
  34. doi request reprint The role of oxygen in wound healing: a review of the literature
    Paola G Rodriguez
    University of Southern California Keck School of Medicine, Los Angeles, California, USA
    Dermatol Surg 34:1159-69. 2008
    ..The presence of oxygen is necessary for normal wound healing. Oxygen has been given as a therapeutic modality to assist and speed wound healing...
  35. ncbi request reprint Hypoxia induces epidermal keratinocyte matrix metalloproteinase-9 secretion via the protein kinase C pathway
    Edel A O'Toole
    Centre for Cutaneous Research, ICMS, Barts and the London School of Medicine and Dentistry, Queen Mary, University of London, London, United Kingdom
    J Cell Physiol 214:47-55. 2008
    ..Inhibition of PKC also inhibited hypoxia-induced keratinocyte migration on type I collagen. These data provide evidence that hypoxia-induced keratinocyte migration is mediated by increased cellular secretion of MMP-9 via the PKC pathway...
  36. ncbi request reprint Drug-induced epidermolysis bullosa acquisita with antibodies to type VII collagen
    Catherine Delbaldo
    Department of Internal Medicine, University Hospital, Geneva, Switzerland
    J Am Acad Dermatol 46:S161-4. 2002
    ....
  37. ncbi request reprint Epidermolysis bullosa acquisita in childhood
    Fanny X Trigo-Guzmán
    Department of Dermatology, University of Sao Paulo, Brazil
    J Dermatol 30:226-9. 2003
    ..After 20 months of initial treatment, clinical remission was observed, and dapsone remains as the current treatment. This case report emphasizes the rarity of EBA in childhood and the difficulties in reaching the final diagnosis...

Research Grants1

  1. Mechanism of Keratinocyte Motility
    David Woodley; Fiscal Year: 2009
    ..Lastly, in our Third Aim, we will develop a novel animal model to examine the veracity of our in vitro data. This model will consist of genetically engineered human skin equivalents grafted onto hairless mice. ..