Douglas B Weibel

Summary

Affiliation: University of Wisconsin
Country: USA

Publications

  1. doi request reprint Bacterial transport of colloids in liquid crystalline environments
    Rishi R Trivedi
    Department of Biochemistry, University of Wisconsin Madison, 440 Henry Mall, Madison, WI 53706, USA
    Soft Matter 11:8404-8. 2015
  2. ncbi request reprint Microfabrication meets microbiology
    Douglas B Weibel
    Department of Biochemistry, University of Wisconsin Madison, 433 Babcock Drive, Madison, Wisconsin 53706, USA
    Nat Rev Microbiol 5:209-18. 2007
  3. ncbi request reprint Applications of microfluidics in chemical biology
    Douglas B Weibel
    Department of Biochemistry, University of Wisconsin Madison, 433 Babcock Drive, Madison, WI 53706, USA
    Curr Opin Chem Biol 10:584-91. 2006
  4. ncbi request reprint Pumping fluids in microfluidic systems using the elastic deformation of poly(dimethylsiloxane)
    Douglas B Weibel
    Department of Biochemistry, University of Wisconsin Madison, 433 Babcock Drive, Madison, WI 53706, USA
    Lab Chip 7:1832-6. 2007
  5. pmc Divin: a small molecule inhibitor of bacterial divisome assembly
    Ye Jin Eun
    Department of Biochemistry, University of Wisconsin Madison, Madison, Wisconsin 53706, USA
    J Am Chem Soc 135:9768-76. 2013
  6. pmc Rapid identification of ESKAPE bacterial strains using an autonomous microfluidic device
    Jack Y Ho
    Department of Biomedical Engineering, University of Wisconsin Madison, Madison, Wisconsin, United States of America
    PLoS ONE 7:e41245. 2012
  7. pmc MinD and MinE interact with anionic phospholipids and regulate division plane formation in Escherichia coli
    Lars D Renner
    Department of Biochemistry, University of Wisconsin, Madison, Wisconsin 53706, USA
    J Biol Chem 287:38835-44. 2012
  8. pmc Measuring the stiffness of bacterial cells from growth rates in hydrogels of tunable elasticity
    Hannah H Tuson
    Department of Biochemistry, University of Wisconsin Madison, Madison, WI 53706, USA
    Mol Microbiol 84:874-91. 2012
  9. pmc Quorum sensing between Pseudomonas aeruginosa biofilms accelerates cell growth
    Shane T Flickinger
    Department of Biochemistry, University of Wisconsin Madison, Madison, Wisconsin 53706, United States
    J Am Chem Soc 133:5966-75. 2011
  10. pmc Encapsulating bacteria in agarose microparticles using microfluidics for high-throughput cell analysis and isolation
    Ye Jin Eun
    Department of Biochemistry, University of Wisconsin Madison, 53706, United States
    ACS Chem Biol 6:260-6. 2011

Collaborators

  • G M Whitesides
  • Kerwyn Casey Huang
  • Arun Yethiraj
  • Martin Thanbichler
  • Kumaran S Ramamurthi
  • Shoji Takeuchi
  • P Garstecki
  • Pedro J Resto
  • Ye Jin Eun
  • Lars D Renner
  • Hannah H Tuson
  • Peter C Mushenheim
  • Manohary Rajendram
  • Rishi R Trivedi
  • Marie H Foss
  • Nicholas L Abbott
  • Matthew F Copeland
  • Katherine A Hurley
  • George K Auer
  • John A Crooks
  • Leili Zhang
  • Piercen M Oliver
  • Jack Y Ho
  • Nate J Cira
  • Shane T Flickinger
  • Adam C Siegel
  • Katherine C Faulkner
  • Saverio E Spagnolie
  • Qiang Cui
  • Jared T Shaw
  • Zhou Zhong
  • Daniela Kiekebusch
  • Abishek Muralimohan
  • Shengqing Xu
  • Molly M Stevens
  • Valerie Huynh
  • Tuan H Nguyen
  • Timothy K Lee
  • Joel S Pendery
  • Gabriella M Nepomuceno
  • Tom R Slezak
  • Clint Torres
  • Sara B Hall
  • Linda I Hu
  • Spencer Cesar
  • Manuel Martinez
  • Thiago M A Santos
  • Jindong Zan
  • Amanda Miguel
  • Michael M Cox
  • Michael S Arnold
  • Rina Maeda
  • Bradley J Reynolds
  • Khanh V Ngo
  • Matthew D Stilwell
  • Susmit Singha Roy
  • Earl J Yoon
  • Alan Saghatelian
  • Jared T Moore
  • Kelsey M Thornton
  • Mathias Leidl
  • Taylor A Wahlig
  • Susan Schlimpert
  • Maoquan Zhou
  • Somenath Bakshi
  • Prahathees Eswaramoorthy
  • Sonia Carey
  • Ryan Sacotte
  • Ajay Gopinathan
  • Max Salick
  • Josue Baeza
  • Wendy C Crone
  • Carolina Tropini
  • Megan E Dueck
  • Daniel A Pauw
  • William M Westler
  • Mariko Hasebe
  • Eric M Downes
  • Andrew T Braasch
  • Andrew S Utada
  • Basudeb Bhattacharyya
  • Andres W Martinez
  • Sergey S Shevkoplyas
  • Derek A Bruzewicz
  • Eugenia Kumacheva
  • Zhihong Nie
  • Minseok Seo
  • Michael Mayer
  • Robert Langer
  • Howard A Stone

Detail Information

Publications40

  1. doi request reprint Bacterial transport of colloids in liquid crystalline environments
    Rishi R Trivedi
    Department of Biochemistry, University of Wisconsin Madison, 440 Henry Mall, Madison, WI 53706, USA
    Soft Matter 11:8404-8. 2015
    ..This study lays the foundation for using cargo-carrying bacteria in engineering applications and for understanding interspecies interactions in polymicrobial communities. ..
  2. ncbi request reprint Microfabrication meets microbiology
    Douglas B Weibel
    Department of Biochemistry, University of Wisconsin Madison, 433 Babcock Drive, Madison, Wisconsin 53706, USA
    Nat Rev Microbiol 5:209-18. 2007
    ....
  3. ncbi request reprint Applications of microfluidics in chemical biology
    Douglas B Weibel
    Department of Biochemistry, University of Wisconsin Madison, 433 Babcock Drive, Madison, WI 53706, USA
    Curr Opin Chem Biol 10:584-91. 2006
    ..The review concludes with an assessment of future directions and opportunities of microfluidics in chemical biology...
  4. ncbi request reprint Pumping fluids in microfluidic systems using the elastic deformation of poly(dimethylsiloxane)
    Douglas B Weibel
    Department of Biochemistry, University of Wisconsin Madison, 433 Babcock Drive, Madison, WI 53706, USA
    Lab Chip 7:1832-6. 2007
    ....
  5. pmc Divin: a small molecule inhibitor of bacterial divisome assembly
    Ye Jin Eun
    Department of Biochemistry, University of Wisconsin Madison, Madison, Wisconsin 53706, USA
    J Am Chem Soc 135:9768-76. 2013
    ..These characteristics make divin a useful probe for studying bacterial cell division and a starting point for the development of new classes of therapeutic agents...
  6. pmc Rapid identification of ESKAPE bacterial strains using an autonomous microfluidic device
    Jack Y Ho
    Department of Biomedical Engineering, University of Wisconsin Madison, Madison, Wisconsin, United States of America
    PLoS ONE 7:e41245. 2012
    ....
  7. pmc MinD and MinE interact with anionic phospholipids and regulate division plane formation in Escherichia coli
    Lars D Renner
    Department of Biochemistry, University of Wisconsin, Madison, Wisconsin 53706, USA
    J Biol Chem 287:38835-44. 2012
    ....
  8. pmc Measuring the stiffness of bacterial cells from growth rates in hydrogels of tunable elasticity
    Hannah H Tuson
    Department of Biochemistry, University of Wisconsin Madison, Madison, WI 53706, USA
    Mol Microbiol 84:874-91. 2012
    ..Our technique therefore enables the rapid determination of how changes in genotype and biochemistry affect the mechanical properties of the bacterial envelope...
  9. pmc Quorum sensing between Pseudomonas aeruginosa biofilms accelerates cell growth
    Shane T Flickinger
    Department of Biochemistry, University of Wisconsin Madison, Madison, Wisconsin 53706, United States
    J Am Chem Soc 133:5966-75. 2011
    ..The results suggest that the secretion of HSLs from a biofilm enhances the growth of neighboring cells in contact with surfaces into communities and may influence their composition, organization, and diversity...
  10. pmc Encapsulating bacteria in agarose microparticles using microfluidics for high-throughput cell analysis and isolation
    Ye Jin Eun
    Department of Biochemistry, University of Wisconsin Madison, 53706, United States
    ACS Chem Biol 6:260-6. 2011
    ..We envision that this technique will have an important impact on research in chemical biology, natural products chemistry, and the discovery and characterization of biologically active secondary metabolites...
  11. doi request reprint Ionic Hydrogen Bonds and Lipid Packing Defects Determine the Binding Orientation and Insertion Depth of RecA on Multicomponent Lipid Bilayers
    Leili Zhang
    Department of Chemistry and Theoretical Chemistry Institute, University of Wisconsin Madison, 1101 University Avenue, Madison, Wisconsin 53706, United States
    J Phys Chem B 120:8424-37. 2016
    ..The different fluorescence behaviors of RecA upon binding to PG- and CL-containing liposomes is likely due to the different structures and flexibility of the C-terminal region of RecA when it binds to different anionic phospholipids. ..
  12. pmc Straining soft colloids in aqueous nematic liquid crystals
    Peter C Mushenheim
    Department of Chemical and Biological Engineering, University of Wisconsin Madison, Madison, WI 53706
    Proc Natl Acad Sci U S A 113:5564-9. 2016
    ..01 mN/m) associated with the GUV-LC interface. Overall, these results provide insight into the coupling of strain in soft materials and suggest previously unidentified designs of LC-based responsive and reconfigurable materials...
  13. pmc Mechanical Genomics Identifies Diverse Modulators of Bacterial Cell Stiffness
    George K Auer
    Department of Biomedical Engineering, University of Wisconsin Madison, Madison, WI 53706, USA
    Cell Syst 2:402-11. 2016
    ..In total, our work demonstrates that bacterial cell stiffness is a property of both the cell wall and broader cell physiology and lays the groundwork for future systematic studies of mechanoregulation. ..
  14. doi request reprint Effects of confinement, surface-induced orientations and strain on dynamical behaviors of bacteria in thin liquid crystalline films
    Peter C Mushenheim
    Department of Chemical and Biological Engineering, University of Wisconsin Madison, 1415 Engineering Drive, Madison, WI 53706, USA
    Soft Matter 11:6821-31. 2015
    ..Overall, these results provide new insight into the influence of surface-oriented LCs on dynamical bacterial behaviors and hint at novel ways to manipulate bacteria using confined LC phases that are not possible in isotropic solutions. ..
  15. pmc Decoding the Chemical Language of Motile Bacteria by Using High-Throughput Microfluidic Assays
    John A Crooks
    Department of Biochemistry, University of Wisconsin Madison, 440 Henry Mall, Madison, WI, 53706, USA
    Chembiochem 16:2151-5. 2015
    ....
  16. pmc Dynamic self-assembly of motile bacteria in liquid crystals
    Peter C Mushenheim
    Department of Chemical and Biological Engineering, University of Wisconsin Madison, 1415 Engineering Drive, Madison, WI 53706, USA
    Soft Matter 10:88-95. 2014
    ....
  17. pmc Anionic Phospholipids Stabilize RecA Filament Bundles in Escherichia coli
    Manohary Rajendram
    Department of Biochemistry, University of Wisconsin Madison, Madison, WI 53706, USA
    Mol Cell 60:374-84. 2015
    ..coli cells lacking aPLs fail to initiate a robust SOS response after DNA damage, indicating that the membrane acts as a scaffold for nucleating the formation of RecA filament bundles and plays an important role in the SOS response...
  18. pmc Polyacrylamide hydrogels as substrates for studying bacteria
    Hannah H Tuson
    Department of Biochemistry, University of Wisconsin Madison, 433 Babcock Drive, Madison, WI 53706, USA
    Chem Commun (Camb) 48:1595-7. 2012
    ..Polyacrylamide hydrogels can be used as chemically and physically defined substrates for bacterial cell culture, and enable studies of the influence of surfaces on cell growth and behaviour...
  19. pmc Using liquid crystals to reveal how mechanical anisotropy changes interfacial behaviors of motile bacteria
    Peter C Mushenheim
    Department of Chemical and Biological Engineering, University of Wisconsin Madison, Madison, Wisconsin
    Biophys J 107:255-65. 2014
    ....
  20. pmc Flagellum density regulates Proteus mirabilis swarmer cell motility in viscous environments
    Hannah H Tuson
    Department of Biochemistry, University of Wisconsin Madison, Madison, WI, USA
    J Bacteriol 195:368-77. 2013
    ..mirabilis flagellum density and cell motility in viscous environments that may be relevant to its adaptation during the infection of mammalian urinary tracts and movement in contact with indwelling catheters...
  21. pmc DCAP: a broad-spectrum antibiotic that targets the cytoplasmic membrane of bacteria
    Ye Jin Eun
    Department of Biochemistry, University of Wisconsin Madison, Madison, Wisconsin 53706, USA
    J Am Chem Soc 134:11322-5. 2012
    ..DCAP expands the limited number of compounds in this class of therapeutic small molecules and provides new opportunities for the development of potent broad-spectrum antimicrobial agents...
  22. doi request reprint Targeting the Bacterial Division Protein FtsZ
    Katherine A Hurley
    Department of Pharmaceutical Sciences, University of Wisconsin Madison, 777 Highland Avenue, Madison, Wisconsin 53705, United States
    J Med Chem 59:6975-98. 2016
    ..This collective information will inform chemists on different aspects of FtsZ that can be (and have been) used to develop successful strategies for devising new families of cell division inhibitors. ..
  23. doi request reprint A self-loading microfluidic device for determining the minimum inhibitory concentration of antibiotics
    Nate J Cira
    Department of Biomedical Engineering, University of Wisconsin Madison, Madison, WI 53706, USA
    Lab Chip 12:1052-9. 2012
    ..As the device introduces a novel approach for filling and isolating dead-end microfluidic chambers that does not require valves and actuators, this technology should find applications in other portable assays and devices...
  24. doi request reprint Chemical-biological studies of subcellular organization in bacteria
    Marie H Foss
    Department of Biochemistry, University of Wisconsin, Madison, Wisconsin 53706, United States
    Biochemistry 50:7719-34. 2011
    ....
  25. doi request reprint Dissecting microbiological systems using materials science
    Abishek Muralimohan
    Department of Biochemistry, University of Wisconsin Madison, 433 Babcock Drive, Madison, WI 53706, USA
    Trends Microbiol 17:100-8. 2009
    ..This review summarizes three classes of materials that span a range of length scales (nano, micro and meso) and describes a variety of fundamental questions in microbiology that can be studied by leveraging their properties...
  26. doi request reprint Fabrication of microbial biofilm arrays by geometric control of cell adhesion
    Ye Jin Eun
    Department of Biochemistry, University of Wisconsin Madison, Madison, WI 53706, USA
    Langmuir 25:4643-54. 2009
    ..The method described in this paper makes it possible to study the chemical, physical, and environmental factors that affect biofilm development in a statistically relevant and reproducible format...
  27. pmc 5-Alkyloxytryptamines are membrane-targeting, broad-spectrum antibiotics
    Katherine C Faulkner
    Department of Biochemistry, University of Wisconsin Madison, 6424A Biochemical Sciences Building, 440 Henry Mall, Madison, WI 53706, USA
    Bioorg Med Chem Lett . 2016
    ....
  28. pmc Detection of ESKAPE bacterial pathogens at the point-of-care using isothermal DNA-based assays in a portable, de-gas microfluidic diagnostic assay platform
    Lars D Renner
    Department of Biochemistry, Department of Biomedical Engineering, Department of Chemistry, University of Wisconsin Madison, Madison, WI 53706
    Appl Environ Microbiol . 2016
    ....
  29. pmc Localization of anionic phospholipids in Escherichia coli cells
    Piercen M Oliver
    Department of Biochemistry, University of Wisconsin Madison, Madison, Wisconsin, USA
    J Bacteriol 196:3386-98. 2014
    ..Framed within the context of these previous experiments, our results suggest that PG may play an essential role in bacterial physiology by maintaining the anionic character of polar membranes. ..
  30. pmc Gyramides prevent bacterial growth by inhibiting DNA gyrase and altering chromosome topology
    Manohary Rajendram
    Department of Biochemistry, Department of Pharmaceutical Sciences, and Department of Chemistry, University of Wisconsin Madison, Madison, Wisconsin 53706, United States
    ACS Chem Biol 9:1312-9. 2014
    ..The specificity and activity of the gyramides for inhibiting gyrase makes these compounds important chemical tools for studying the mechanism of gyrase and the connection between DNA topology and bacterial cell division...
  31. pmc Studying biomolecule localization by engineering bacterial cell wall curvature
    Lars D Renner
    Department of Biochemistry, University of Wisconsin Madison, Madison, Wisconsin, United States of America Technical University Dresden and the Max Bergmann Centre for Biomaterials, Dresden, Germany
    PLoS ONE 8:e84143. 2013
    ..These studies highlight a unique approach for studying the relationship between cell shape and intracellular organization in intact, live bacteria. ..
  32. pmc Cardiolipin microdomains localize to negatively curved regions of Escherichia coli membranes
    Lars D Renner
    Department of Biochemistry and Biomedical Engineering, University of Wisconsin, Madison, WI 53706, USA
    Proc Natl Acad Sci U S A 108:6264-9. 2011
    ..These studies demonstrate the curvature dependence of CL in membranes and test whether these structures participate in the localization of MinD to regions of negative curvature in cells...
  33. pmc Oligochlorophens are potent inhibitors of Bacillus anthracis
    Marie H Foss
    Department of Biochemistry, University of Wisconsin Madison, Madison, WI 53706 1544, USA
    Antimicrob Agents Chemother 54:3988-90. 2010
    ..B. anthracis develops resistance to the oligochlorophens at a rate of 4.34 x 10(-10) per generation, which is approximately 10-fold lower than that of commercial antibiotics used to treat this human pathogen...
  34. pmc Studying the dynamics of flagella in multicellular communities of Escherichia coli by using biarsenical dyes
    Matthew F Copeland
    Department of Biochemistry, University of Wisconsin Madison, 433 Babcock Drive, Madison, WI 53706, USA
    Appl Environ Microbiol 76:1241-50. 2010
    ..This approach opens a window for observing the dynamics of cells in communities that are relevant to ecology, industry, and biomedicine...
  35. ncbi request reprint Cofabrication of electromagnets and microfluidic systems in poly(dimethylsiloxane)
    Adam C Siegel
    Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford Street, Cambridge, MA 02138, USA
    Angew Chem Int Ed Engl 45:6877-82. 2006
  36. ncbi request reprint Combining microscience and neurobiology
    Douglas B Weibel
    Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford Street, Cambridge, MA 02138, USA
    Curr Opin Neurobiol 15:560-7. 2005
    ..The major impediment to the development of a field of 'microfabrication and measurement' in neuroscience is the absence of effective collaborative interactions between the communities of fabricators and neurobiologists...
  37. ncbi request reprint Modeling the anodic half-cell of a low-temperature coal fuel cell
    Douglas B Weibel
    Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA
    Angew Chem Int Ed Engl 44:5682-6. 2005
  38. ncbi request reprint Torque-actuated valves for microfluidics
    Douglas B Weibel
    Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts 02138, USA
    Anal Chem 77:4726-33. 2005
    ..They also allow settings between "on" and "off" and can be integrated into portable, disposable microfluidic devices for carrying out sandwich immunoassays...
  39. ncbi request reprint Direct patterning of mammalian cells onto porous tissue engineering substrates using agarose stamps
    Molly M Stevens
    Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Biomaterials 26:7636-41. 2005
    ..This approach may find use in controlling the spatial invasion of scaffolds, promoting the hierarchical organization of cells, and in controlling cell-cell interactions as a step in preservation of phenotypes of cells...
  40. ncbi request reprint Generation of monodisperse particles by using microfluidics: control over size, shape, and composition
    Shengqing Xu
    Department of Chemistry, University of Toronto, 80 Saint George Street, Toronto M5S 3H6, Ontario, Canada
    Angew Chem Int Ed Engl 44:724-8. 2005