Carol B Ware

Summary

Affiliation: University of Washington
Country: USA

Publications

  1. ncbi Utility of a C57BL/6 ES line versus 129 ES lines for targeted mutations in mice
    Carol B Ware
    Department of Comparative Medicine, University of Washington, Box 357190, Seattle, WA 98195 7190, USA
    Transgenic Res 12:743-6. 2003
  2. ncbi Cryopreservation of rhesus macaque embryonic stem cells
    Szczepan W Baran
    Department of Comparative Medicine, University of Washington, Seattle, WA 98195, USA
    Stem Cells Dev 16:339-44. 2007
  3. pmc Diverse hematopoietic potentials of five human embryonic stem cell lines
    Kai Hsin Chang
    Department of Medicine, Hematology Division, University of Washington, 1705 NE Pacific Street, Box 357710, Seattle, WA, USA
    Exp Cell Res 314:2930-40. 2008
  4. pmc SirT1 modulates the estrogen-insulin-like growth factor-1 signaling for postnatal development of mammary gland in mice
    Hongzhe Li
    Department of Radiation Oncology, University of Washington School of Medicine, Seattle, WA 98195, USA
    Breast Cancer Res 9:R1. 2007
  5. ncbi A comparison of NIH-approved human ESC lines
    Carol B Ware
    Department of Comparative Medicine, University of Washington, Box 357190, Seattle, Washington 98195, USA
    Stem Cells 24:2677-84. 2006
  6. pmc Histone deacetylase inhibition elicits an evolutionarily conserved self-renewal program in embryonic stem cells
    Carol B Ware
    Institute for Stem Cell and Regenerative Medicine, University of Washington, Seattle, WA 98195, USA
    Cell Stem Cell 4:359-69. 2009
  7. ncbi Controlled-rate freezing of human ES cells
    Carol B Ware
    Department of Comparative Medicine, University of Washington, Seattle, WA 98195 7190, USA
    Biotechniques 38:879-80, 882-3. 2005
  8. pmc Late gestation modulation of fetal glucocorticoid effects requires the receptor for leukemia inhibitory factor: an observational study
    Carol B Ware
    Department of Comparative Medicine, University of Washington, Seattle, WA 98195 7190, USA
    Reprod Biol Endocrinol 1:43. 2003
  9. ncbi Glutamate cysteine ligase modifier subunit deficiency and gender as determinants of acetaminophen-induced hepatotoxicity in mice
    Lisa A McConnachie
    Department of Environmental and Occupational Health Sciences, University of Washington, Seattle, Washington 98195, USA
    Toxicol Sci 99:628-36. 2007
  10. ncbi Leukemia inhibitory factor signaling is implicated in embrionic development of the HPA axis
    Carol B Ware
    Department of Comparative Medicine, University of Washington, Seattle, WA 98195 7190, USA
    FEBS Lett 579:4465-9. 2005

Collaborators

Detail Information

Publications28

  1. ncbi Utility of a C57BL/6 ES line versus 129 ES lines for targeted mutations in mice
    Carol B Ware
    Department of Comparative Medicine, University of Washington, Box 357190, Seattle, WA 98195 7190, USA
    Transgenic Res 12:743-6. 2003
    ..Thus, although less efficient than 129 ES lines, the C57BL/6 ES line is a relatively competent line and useful for the routine generation of targeted mutations in mice on a defined genetic background...
  2. ncbi Cryopreservation of rhesus macaque embryonic stem cells
    Szczepan W Baran
    Department of Comparative Medicine, University of Washington, Seattle, WA 98195, USA
    Stem Cells Dev 16:339-44. 2007
    ..Preserved cells retained a normal karyotype and did not lose their ability to express markers of undifferentiated ES cells...
  3. pmc Diverse hematopoietic potentials of five human embryonic stem cell lines
    Kai Hsin Chang
    Department of Medicine, Hematology Division, University of Washington, 1705 NE Pacific Street, Box 357710, Seattle, WA, USA
    Exp Cell Res 314:2930-40. 2008
    ..The identification of intrinsic and extrinsic factors governing the hematopoietic differentiation potential of hESCs will be of great importance for the putative clinical utility of hESC lines...
  4. pmc SirT1 modulates the estrogen-insulin-like growth factor-1 signaling for postnatal development of mammary gland in mice
    Hongzhe Li
    Department of Radiation Oncology, University of Washington School of Medicine, Seattle, WA 98195, USA
    Breast Cancer Res 9:R1. 2007
    ..Whether SirT1 regulates the IGF-1 signaling for mammary gland development and function, however, is not clear. In the present study, this role of SirT1 was examined by studying SirT1-deficient mice...
  5. ncbi A comparison of NIH-approved human ESC lines
    Carol B Ware
    Department of Comparative Medicine, University of Washington, Box 357190, Seattle, Washington 98195, USA
    Stem Cells 24:2677-84. 2006
    ..Many hESC lines can be maintained using culture conditions less onerous than those recommended by their suppliers. Intellectual property issues pose a significant obstacle to research using NIH-approved hESC lines...
  6. pmc Histone deacetylase inhibition elicits an evolutionarily conserved self-renewal program in embryonic stem cells
    Carol B Ware
    Institute for Stem Cell and Regenerative Medicine, University of Washington, Seattle, WA 98195, USA
    Cell Stem Cell 4:359-69. 2009
    ..Our results indicate that HDACi can promote ESC self-renewal across species, and demonstrate that ESCs can toggle between alternative states in response to environmental factors...
  7. ncbi Controlled-rate freezing of human ES cells
    Carol B Ware
    Department of Comparative Medicine, University of Washington, Seattle, WA 98195 7190, USA
    Biotechniques 38:879-80, 882-3. 2005
    ..3 degrees and 1.8 degrees C/min, and a rapid thaw rate using room temperature water. Slow controlled-rate cooling allows a rapid, simple, and reproducible means of cryopreserving hESCs...
  8. pmc Late gestation modulation of fetal glucocorticoid effects requires the receptor for leukemia inhibitory factor: an observational study
    Carol B Ware
    Department of Comparative Medicine, University of Washington, Seattle, WA 98195 7190, USA
    Reprod Biol Endocrinol 1:43. 2003
    ..Uncovering a requirement for LIFR in appropriate glucocorticoid response will further understanding of control of glucocorticoid function...
  9. ncbi Glutamate cysteine ligase modifier subunit deficiency and gender as determinants of acetaminophen-induced hepatotoxicity in mice
    Lisa A McConnachie
    Department of Environmental and Occupational Health Sciences, University of Washington, Seattle, Washington 98195, USA
    Toxicol Sci 99:628-36. 2007
    ..Collectively, these data reassert the importance of GSH in protection against APAP-induced hepatotoxicity, and indicate critical roles for GCL activity and gender in APAP-induced liver damage in mice...
  10. ncbi Leukemia inhibitory factor signaling is implicated in embrionic development of the HPA axis
    Carol B Ware
    Department of Comparative Medicine, University of Washington, Seattle, WA 98195 7190, USA
    FEBS Lett 579:4465-9. 2005
    ..Lifr -/- mice demonstrate elevated pituitary glucocorticoid (GR) and mineralocorticoid (MR) receptor mRNA and protein levels, indicating the importance of LIF signaling for HPA axis development...
  11. pmc Derivation of naive human embryonic stem cells
    Carol B Ware
    Institute for Stem Cell and Regenerative Medicine, Departments of Comparative Medicine, Biology, and Biochemistry, and Divisions of Hematology, Metabolism, Endocrinology and Nutrition, and Medical Genetics, Department of Medicine, University of Washington, Seattle, WA 98195
    Proc Natl Acad Sci U S A 111:4484-9. 2014
    ..Direct derivation is an elusive, but attainable, process, leading to cells at the earliest stage of in vitro pluripotency described for humans. Reverse toggling of primed cells to naïve is efficient and reproducible. ..
  12. pmc Definitive-like erythroid cells derived from human embryonic stem cells coexpress high levels of embryonic and fetal globins with little or no adult globin
    Kai Hsin Chang
    Division of Hematology, Department of Medicine, University of Washington, 1705 NE Pacific, Rm K243, Box 357710, Seattle, WA 98195 7710, USA
    Blood 108:1515-23. 2006
    ..Nevertheless, the high frequency of erythroid cells coexpressing embryonic and fetal globin generated from embryonic stem cells can serve as an invaluable tool to further explore molecular mechanisms...
  13. ncbi A controlled-cooling protocol for cryopreservation of human and non-human primate embryonic stem cells
    Carol B Ware
    Department of Comparative Medicine, University of Washington, Seattle, USA
    Methods Mol Biol 407:43-9. 2007
    ..Thus, this protocol describes a technically simple but effective means of long-term storage of primate ES cells...
  14. doi Conversion of Prostate Adenocarcinoma to Small Cell Carcinoma-Like by Reprogramming
    Gisely T Borges
    Department of Urology, University of Washington, Seattle, Washington
    J Cell Physiol 231:2040-7. 2016
    ..Prostate cancer progression can thus be molecularly characterized by loss of differentiation with re-expression of stem cell genes. J. Cell. Physiol. 231: 2040-2047, 2016. © 2016 Wiley Periodicals, Inc. ..
  15. pmc Fine-scale chromatin interaction maps reveal the cis-regulatory landscape of human lincRNA genes
    Wenxiu Ma
    Department of Genome Sciences, University of Washington, Seattle, Washington, USA
    Nat Methods 12:71-8. 2015
    ..Our results provide the first glimpse of the cell type-specific 3D organization of lincRNA genes. ..
  16. doi Reprogramming of prostate cancer-associated stromal cells to embryonic stem-like
    Eneida F Vencio
    Department of Urology and Institute for Stem Cell and Regenerative Medicine, University of Washington, Seattle, Washington, USA
    Prostate 72:1453-63. 2012
    ..This technology might be used to 'erase' the abnormal gene expression of diseased cells. The resultant iPS cells would no longer express the disease phenotype, and behave like stem cells...
  17. pmc Efficient generation of retinal progenitor cells from human embryonic stem cells
    Deepak A Lamba
    Department of Biological Structure and Comparative Medicine, University of Washington, Seattle, WA 98195, USA
    Proc Natl Acad Sci U S A 103:12769-74. 2006
    ..These results demonstrate that human ES cells can be selectively directed to a neural retinal cell fate and thus may be useful in the treatment of retinal degenerations...
  18. pmc Self-renewal of human embryonic stem cells requires insulin-like growth factor-1 receptor and ERBB2 receptor signaling
    Linlin Wang
    Division of Hematology, Department of Medicine, Institute for Stem Cell and Regenerative Medicine, University of Washington, Seattle, 98195, USA
    Blood 110:4111-9. 2007
    ..These studies identify previously unappreciated RTKs that support hESC proliferation and self-renewal, and provide a rationally designed medium for the growth and maintenance of pluripotent hESCs...
  19. ncbi A knock-in mouse model of gastrointestinal stromal tumor harboring kit K641E
    Brian P Rubin
    Department of Pathology, University of Washington Medical Center, Seattle, Washington 98195, USA
    Cancer Res 65:6631-9. 2005
    ....
  20. pmc Lineage relationship of prostate cancer cell types based on gene expression
    Laura E Pascal
    Department of Urology University of Washington, Seattle, WA 98195, USA
    BMC Med Genomics 4:46. 2011
    ..Cell type-specific transcriptomes are used to examine lineage relationship among cancer cell types and their expression similarity to normal cell types including stem/progenitor cells...
  21. ncbi Transduction of human embryonic stem cells by foamy virus vectors
    Helen Gharwan
    Department of Medicine, University of Washington, Seattle, Washington 98195, USA
    Mol Ther 15:1827-33. 2007
    ..Thus, FV vectors are promising tools for the genetic modification of hESCs...
  22. ncbi Transplantation of undifferentiated murine embryonic stem cells in the heart: teratoma formation and immune response
    Jeannette Nussbaum
    Department of Pathology, University of Washington, Seattle, WA, USA
    FASEB J 21:1345-57. 2007
    ..Successful cardiac repair strategies involving ES cells will need to control cardiac differentiation, avoid introducing undifferentiated cells, and will likely require immune modulation to avoid rejection...
  23. ncbi Acetaminophen-induced liver injury is attenuated in male glutamate-cysteine ligase transgenic mice
    Dianne Botta
    Department of Environmental and Occupational Health Sciences, Comparative Medicine, and Pathology, and UW NIEHS Center for Ecogenetics and Environmental Health, University of Washington, Seattle, Washington 68105, USA
    J Biol Chem 281:28865-75. 2006
    ..Because people are known to vary in their glutamate-cysteine ligase activity, this enzyme may also be an important determinant of sensitivity to acetaminophen-induced liver injury in humans...
  24. pmc The GIPC1-Akt1 Pathway Is Required for the Specification of the Eye Field in Mouse Embryonic Stem Cells
    Anna La Torre
    Department of Biological Structure, Institute for Stem Cells and Regenerative Medicine, University of Washington, Seattle, Washington, USA
    Stem Cells 33:2674-85. 2015
    ..Our data, together with previous studies in Xenopus, support the hypothesis that the GIPC1-PI3K-Akt1 pathway plays a key role in eye field specification in vertebrates...
  25. ncbi Isoform-specific knockout of FE65 leads to impaired learning and memory
    Baiping Wang
    Department of Pathology, University of Washington, Seattle, Washington 98195 7470, USA
    J Neurosci Res 75:12-24. 2004
    ..Reduced secretion of Abeta peptides was observed in primary neuronal cultures of hybrids of p97FE65(-/-)/betaPP transgenic (Tg2576) mice. These studies suggest an important and novel function of FE65 in learning and memory...
  26. ncbi Genomic context drives SCA7 CAG repeat instability, while expressed SCA7 cDNAs are intergenerationally and somatically stable in transgenic mice
    Randell T Libby
    Department of Laboratory Medicine, University of Washington Medical Center, Seattle, WA 98195 7110, USA
    Hum Mol Genet 12:41-50. 2003
    ..As large somatic repeat expansions are absent from the brains of SCA7 cDNA mice, our results indicate that neurodegeneration can occur without marked somatic mosaicism, at least in these mice...
  27. ncbi Polyglutamine-expanded ataxin-7 promotes non-cell-autonomous purkinje cell degeneration and displays proteolytic cleavage in ataxic transgenic mice
    Gwenn A Garden
    Department of Neurology, University of Washington Medical Center, Seattle, Washington 98195 7110, USA
    J Neurosci 22:4897-905. 2002
    ..The non-cell-autonomous nature of the Purkinje cell degeneration in our SCA7 mouse model indicates that polyglutamine-induced dysfunction in adjacent or connecting cell types contributes to the neurodegeneration...
  28. ncbi Androgen receptor YAC transgenic mice recapitulate SBMA motor neuronopathy and implicate VEGF164 in the motor neuron degeneration
    Bryce L Sopher
    Department of Laboratory Medicine, University of Washington Medical Center, Seattle, WA 98195 USA
    Neuron 41:687-99. 2004
    ..Our results suggest that SBMA motor neuronopathy involves altered expression of VEGF, consistent with a role for VEGF as a neurotrophic/survival factor in motor neuron disease...